Rh4BG090500

vascular transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011356
Physical Location & Seq
Reverse (-)
15497585 .. 15501805
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG090500.1

Sequence Viewer

Length: 3174 bp
ATGCGCTCAGGAGGTTGTGCGGTGCAGCAGACCCTCACAGCGGAGGCTGCTTCGGTTCTGAAGCACTCTCTCAGCTTAGCCAGGCGGAGAGGCCATGCTCAGGTCACTCCTCTCCACGTGGCCGCCACTCTCCTCGCCTCAAGAACCAGTCTCTTGAGGCGGGCCTGTCTCAAAGCTGCCGCAGCAGCCAACCACAACCCGCCACATCACCACCCTCTCCAATGCAGGGCCCTGGAGCTCTGCTTCAATGTGGCGCTTAACAGGCTCCCCACTACACCTCCCTCAGGAGGTGGCGGCGCCGCCAGTCCTCTGCTGGTCAACCACGGTCAGCACCACCAGCAGCAACAGCCTTCTCTCTCCAATGCTCTCATTGCAGCTCTCAAAAGGGCCCAAGCCCACCAGAGAAGGGGGTGCATAGAGCAGCAGAACCAGCAGCAGCCACTTTTGACGATCAAGGTTGAGCTTGAGCAGCTGATCATCTCCATCTTGGATGACCCAAGTGTTAGCAGGGTTATGAGGGAAGCCGGGTTCTCAAGTACTTCTGTCAAGAACAACTTAGAGGACACCTCCTCTGTTTCCTCTGTTTTCCAGTGTTATAATAGCACTGGTGGGGTTTTCTCTTCTCCTTGTTCACCTACTGCTCCTGAAAACTTAACCCATCATCATATCAGTCCATCTGGGAATTTCTGGCAAACCCATTTCTTGACTTACACTTCTGAGCAAAACCCACTTCTCTTTTCCTCCCCACCAAAGAAATTACTTCTTCCATTCAATGTCAGCTCCATTAATACTCCTACAGAGTCTGTCTCTTATTCCAAGGAAGATGTTAAGTTGGTGTTTGAGGTTTTGGTGAGGAAGAACAGTAAGAAAAGGAACACTGTGATTGTGGGTGATTTTATGTCCATTACTGAAGGCCTTGTTGCTGAGGTCATGGGGAGGTTAGAGAGAGGGTCAGAGGTTCCTGAGGAACTGAAATCAACCCATTTCATCAAATTTCAGTTTCCACATGTTTCTTTGAGGTACATGAAAAGAGAAGATGTGGAATCAAAAATCACAGAGCTCAAAAGGAAATTGGATCACTCTGGAGGAGGTGGTGCTATTATTTACATTGGAGACTTGAAATGGACAATTGGTGATGAGAGAGAAGGAGGATTAGTCTCTGGTGGGTATAGCCCAGTTGAGCATTTAGTTTCAGAAATTTCAAGGTTGGTTTCAGATTATGAAAGCAGCTCATGCTCAAGCACAAAGCCAAAGGTGTGGCTAATGGCTACTGCTAGTTATCAAACATACATGAGATGCCAAATGAGGCAACCTTCTCTTGAGATTCAATGGGGTCTTCAAGCTGTTTCAGTACCATCAGGAGGTCTTGGTTTGAGTCTCCATGCTTCCAGTGTCCATGATTCGAGAGTAATCTTTTCTCAAAACTCATCTGAAGTGCTTGAAACAAAGCCATTCAGTAGCATCAAGGATCGTGAACAAGATCATGGTAAGCTCCCCTGCTGTGAAGAATGCACTTCAAATTATGAGAAAGAAGCTCAGTTGCTCAAATCTGGCCAGCAGAAATTGCCTGCCTGGCTTCAACCACATGGCACCCAAGCCTGTCAAAAGGATGAAGTGGTTGAATTGAGGAGAAAGTGGAACAGGTTATGCTACAGTCTGCACCAAGGAAGGCATAATCAGAATCATTCAAGCCCCGCTTTGTACAACCATCAAAACTCAATTGGGAAGAACCACTCTTATAGTACTTCATCGTACCCTTGGTTGTCAACCAGGAATGGCATCTTCCCAGATCTGAATTCGATCTCTTTTGCTGATCATCCAGCTTCAGACCCTGCAACCCATGGCTCTAATCTGGTGCCTCGGTTCAGGAGACAACAATCATGTAGTACAATCGAATTCAATTTCGATAATGGGGTTCAAAAGCATGAAGTAGTGGAACCAACCTTGGATTCTTTGAAACTCAGTGAAGATAAGGAAGTGAAGATCACACTTGCTCTCGGCAACTCTGTGTTTTCTGATTCAGGAAAATCGATGGTGCAGAGAGCTGACATGTGTAAGCTTTTGAAAGAGAATGTGCCTTGGCAATCTGAATCCATTCCCTCAATAGTAGAAGCCATAATCGGCTCCAAACCATGTTCGGAAACTTGGTTTCTGATTGATGGGAATGACTCGATTGGAAAGAGAAGGTTGGCTCAAGCAATAGCAGAGTTGGTTCTGGGATCTGCTAATTCACTCTTACATATCAACATGAACAAAAGAGAAGATGAGATGTACCCAAGAGTAGAAAAACTAAAAAAAGCCTTGAAATCCAGTGACAAACTTGTTGTTTTGGTAGAAGATGTTGATTTGGCTGATACCCAGTTCTTAAAATTCCTAGCTGATCGAAAATGTGGAGAAGTGAGTAGAAGAGATGGGAATCAAGGCCAAGCAATATTCATTTTGACCAAGGGTGAGTCCACAAGAAATGAGTACCCAGGTTCTATAATCCAAATGAAGTTGAAAGTTGATGAAAAAAGTACTAGTCCTAGTTTTGGGATCGCCAGCTTCGAACACAAGCGAAAAGCTGAGTGGGAGCTTGAAAACAAGACCAAATCTACAAGATTTGAAGAAAAGGAGGATTCAAGTGTGGTTGTTGCCTTTGAGAATGTGAATAGCAAGAAGGACTTCTCAAGGCAATCAAGCTTCAACAGTAACCTTGATCTAAACCTCAAAGCCGGTGAGGACAATGAAATCGAAGACAATGCAGGGGAGGTTAGCCCTATTTCAAGTGATTTGACCCGCGACTCTGCCACGGATGTCCAAACCCCACTTGGGTTCCTCGAATCAGTCGAGAACCTCTTCGTTTTCAATCGAAGCCCAGCTAGAGATCGGGAGGCCACCGAGCTTTTCTTGTCCAAGATTGAGGGGTGCTTTGAGGGTGTATATGGGAAACAAAATGGGGTTAGTTTTAGTGTGGATAAGAGGGTGTTAGAAGAGATATCTGTTGGGTCTGGTTCTTTTCCCAATAGCTTGTTTGAGAAATGGCTAGAAGGTATTTTTCAAACAAGCCTAAAATCGGTTAAATTTGGCGGGAAGGAGGGTATACTTGTAAGGCTATGTTTGGGGGGTACAGAAGAAGGCATTTTGGAGGGTTTCTTGGGCTCTTGTCTTCCCAAGAAAATCCAAATTTCTTGA

Protein Analysis

1057

Amino Acids

117.0

Weight (kDa)

7.2

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NBD_SMAX1 PF23569 272 - 464 9e-80 SMAX1 nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29920 AT4G29920
fragaria_vesca FvH4_4g07050 FvH4_4g07050
malus_domestica MD00G1086800.v1.1 MD16G1247100.v1.1
prunus_persica Prupe.1G073700_v2.0.a1
pyrus_communis pycom13g21290 pycom16g20740
rosa_chinensis RchiOBHm_Chr4g0399171
rosa_laevigata RLG00000009293
rosa_multiflora Rmu_sc0002062.1_g000001 Rmu_sc0006088.1_g000002
rosa_roxburghii Rroxscaffold_5G00344130
rosa_rugosa Rorug04G0014600
rosa_samantha Rh4AG092500 Rh4BG090500 Rh4CG101000 Rh4DG084900
rosa_wichuraiana Rw4G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AccB1I GGYRCC 3 cut(s) 296, 1589, 1855
AccI GTMKAC 1 cut(s) 3082
AccII CGCG 1 cut(s) 2781
AclWI GGATC 4 cut(s) 1083, 1476, 2227, 2543
AcoI YGGCCR 2 cut(s) 120, 1552
AcsI RAATTY 8 cut(s) 682, 992, 1197, 1795, 1895, 2369, 3062, 3165
AcuI CTGAAG 4 cut(s) 80, 930, 1452, 1809
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 297
AflIII ACRYGT 2 cut(s) 1006, 2049
AhlI ACTAGT 1 cut(s) 2519
AjnI CCWGG 5 cut(s) 80, 231, 1571, 1769, 2473
AjuI GAANNNNNNNTTGG 2 cut(s) 1933, 1965
AloI GAACNNNNNNTCC 2 cut(s) 512, 544
Alw21I GWGCWC 2 cut(s) 240, 1062
Alw26I GTCTC 7 cut(s) 155, 173, 811, 1107, 1162, 1382, 1864
AlwI GGATC 4 cut(s) 1083, 1476, 2227, 2543
AlwNI CAGNNNCTG 2 cut(s) 803, 1832
AoxI GGCC 9 cut(s) 91, 120, 162, 228, 387, 913, 1552, 2422, 2874
ApaI GGGCCC 2 cut(s) 232, 391
ApoI RAATTY 8 cut(s) 682, 992, 1197, 1795, 1895, 2369, 3062, 3165
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 3 cut(s) 2094, 2663, 2837
AspLEI GCGC 3 cut(s) 6, 256, 299
AspS9I GGNCC 5 cut(s) 162, 228, 229, 387, 388
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 7 cut(s) 200, 624, 862, 902, 1145, 2462, 2729
AsuII TTCGAA 1 cut(s) 2547
AxyI CCTNAGG 2 cut(s) 283, 963
BaeGI GKGCMC 2 cut(s) 232, 391
BalI TGGCCA 1 cut(s) 1554
BanI GGYRCC 3 cut(s) 296, 1589, 1855
BanII GRGCYC 5 cut(s) 232, 240, 391, 1062, 3143
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 3 cut(s) 1328, 2742, 3140
Bbv12I GWGCWC 2 cut(s) 240, 1062
BbvCI CCTCAGC 1 cut(s) 924
BccI CCATC 8 cut(s) 491, 666, 682, 1363, 1716, 2026, 2153, 2405
BcgI CGANNNNNNTGC 2 cut(s) 2723, 2757
BciT130I CCWGG 5 cut(s) 82, 233, 1573, 1771, 2475
BclI TGATCA 2 cut(s) 474, 1813
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 7 cut(s) 155, 173, 811, 1107, 1162, 1382, 1864
BcuI ACTAGT 1 cut(s) 2519
BfaI CTAG 6 cut(s) 1275, 2375, 2520, 2526, 2862, 3026
BfmI CTRYAG 2 cut(s) 795, 1651
BfoI RGCGCY 2 cut(s) 257, 300
BglI GCCNNNNNGGC 1 cut(s) 1573
BglII AGATCT 1 cut(s) 1789
BlpI GCTNAGC 1 cut(s) 76
BmcAI AGTACT 3 cut(s) 538, 1744, 2518
Bme1390I CCNGG 6 cut(s) 82, 233, 526, 1573, 1771, 2475
BmgT120I GGNCC 5 cut(s) 162, 228, 229, 387, 388
BmrFI CCNGG 6 cut(s) 82, 233, 526, 1573, 1771, 2475
BmrI ACTGGG 2 cut(s) 1169, 2353
BmsI GCATC 3 cut(s) 1286, 1470, 1788
BmuI ACTGGG 2 cut(s) 1169, 2353
BpiI GAAGAC 3 cut(s) 1328, 2742, 3140
BplI GAGNNNNNCTC 4 cut(s) 551, 583, 791, 823
BpmI CTGGAG 2 cut(s) 254, 1104
Bpu10I CCTNAGC 3 cut(s) 7, 99, 924
Bpu1102I GCTNAGC 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 2547
BpuEI CTTGAG 8 cut(s) 124, 175, 485, 517, 1222, 1340, 2178, 2653
BpuMI CCSGG 1 cut(s) 526
Bsa29I ATCGAT 1 cut(s) 2030
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 2415
BsaHI GRCGYC 1 cut(s) 297
BsaXI ACNNNNNCTCC 4 cut(s) 262, 292, 1140, 1170
Bse118I RCCGGY 1 cut(s) 2714
Bse1I ACTGG 8 cut(s) 147, 303, 589, 610, 1175, 1389, 2310, 2359
Bse21I CCTNAGG 2 cut(s) 283, 963
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 2415
BseBI CCWGG 5 cut(s) 82, 233, 1573, 1771, 2475
BseCI ATCGAT 1 cut(s) 2030
BseGI GGATG 4 cut(s) 496, 1615, 1816, 2800
BseJI GATNNNNATC 1 cut(s) 2415
BseMI GCAATG 1 cut(s) 369
BseNI ACTGG 8 cut(s) 147, 303, 589, 610, 1175, 1389, 2310, 2359
BseRI GAGGAG 5 cut(s) 99, 122, 559, 1101, 1642
BseSI GKGCMC 2 cut(s) 232, 391
BseYI CCCAGC 1 cut(s) 2857
BsgI GTGCAG 3 cut(s) 44, 1643, 2057
Bsh1236I CGCG 1 cut(s) 2781
BshFI GGCC 9 cut(s) 93, 122, 164, 230, 389, 915, 1554, 2424, 2876
BshNI GGYRCC 3 cut(s) 296, 1589, 1855
BshVI ATCGAT 1 cut(s) 2030
BsiHKAI GWGCWC 2 cut(s) 240, 1062
BsiSI CCGG 2 cut(s) 525, 2715
BsmAI GTCTC 7 cut(s) 155, 173, 811, 1107, 1162, 1382, 1864
BsmI GAATGC 1 cut(s) 1514
BsnI GGCC 9 cut(s) 93, 122, 164, 230, 389, 915, 1554, 2424, 2876
Bsp119I TTCGAA 1 cut(s) 2547
Bsp120I GGGCCC 2 cut(s) 228, 387
Bsp1286I GDGCHC 5 cut(s) 232, 240, 391, 1062, 3143
Bsp1407I TGTACA 1 cut(s) 1701
Bsp1720I GCTNAGC 1 cut(s) 76
Bsp19I CCATGG 1 cut(s) 1840
BspANI GGCC 9 cut(s) 93, 122, 164, 230, 389, 915, 1554, 2424, 2876
BspDI ATCGAT 1 cut(s) 2030
BspFNI CGCG 1 cut(s) 2781
BspPI GGATC 4 cut(s) 1083, 1476, 2227, 2543
BspT104I TTCGAA 1 cut(s) 2547
BspT107I GGYRCC 3 cut(s) 296, 1589, 1855
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 2714
BsrGI TGTACA 1 cut(s) 1701
BsrI ACTGG 8 cut(s) 147, 303, 589, 610, 1175, 1389, 2310, 2359
BssAI RCCGGY 1 cut(s) 2714
BssNAI GTATAC 1 cut(s) 3083
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 7 cut(s) 816, 1663, 1757, 1840, 1944, 2078, 2445
Bst1107I GTATAC 1 cut(s) 3083
Bst2UI CCWGG 5 cut(s) 82, 233, 1573, 1771, 2475
Bst4CI ACNGT 5 cut(s) 326, 863, 880, 1655, 2690
Bst6I CTCTTC 4 cut(s) 625, 2401, 2843, 2967
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 2 cut(s) 1233, 1564
BstAUI TGTACA 1 cut(s) 1701
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 2547
BstC8I GCNNGC 4 cut(s) 162, 1556, 1569, 2542
BstDSI CCRYGG 3 cut(s) 322, 1840, 2790
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 496, 1615, 1816, 2800
BstFNI CGCG 1 cut(s) 2781
BstH2I RGCGCY 2 cut(s) 257, 300
BstHHI GCGC 3 cut(s) 6, 256, 299
BstMAI GTCTC 7 cut(s) 155, 173, 811, 1107, 1162, 1382, 1864
BstNI CCWGG 5 cut(s) 82, 233, 1573, 1771, 2475
BstNSI RCATGY 2 cut(s) 1010, 2053
BstSCI CCNGG 6 cut(s) 80, 231, 524, 1571, 1769, 2473
BstSFI CTRYAG 2 cut(s) 795, 1651
BstSLI GKGCMC 2 cut(s) 232, 391
BstUI CGCG 1 cut(s) 2781
BstV2I GAAGAC 3 cut(s) 1328, 2742, 3140
BstX2I RGATCY 2 cut(s) 1789, 2219
BstXI CCANNNNNNTGG 1 cut(s) 1257
BstYI RGATCY 2 cut(s) 1789, 2219
BstZ17I GTATAC 1 cut(s) 3083
Bsu15I ATCGAT 1 cut(s) 2030
Bsu36I CCTNAGG 2 cut(s) 283, 963
BsuRI GGCC 9 cut(s) 93, 122, 164, 230, 389, 915, 1554, 2424, 2876
BsuTUI ATCGAT 1 cut(s) 2030
BtgI CCRYGG 3 cut(s) 322, 1840, 2790
BtsCI GGATG 4 cut(s) 496, 1615, 1816, 2800
BtsIMutI CAGTG 6 cut(s) 596, 603, 876, 1396, 1969, 2317
Cac8I GCNNGC 4 cut(s) 162, 1556, 1569, 2542
CaiI CAGNNNCTG 2 cut(s) 803, 1832
CfoI GCGC 3 cut(s) 6, 256, 299
Cfr10I RCCGGY 1 cut(s) 2714
Cfr13I GGNCC 5 cut(s) 162, 228, 229, 387, 388
ClaI ATCGAT 1 cut(s) 2030
DinI GGCGCC 1 cut(s) 298
EaeI YGGCCR 2 cut(s) 120, 1552
Eam1104I CTCTTC 4 cut(s) 625, 2401, 2843, 2967
EarI CTCTTC 4 cut(s) 625, 2401, 2843, 2967
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 2 cut(s) 238, 1060
Eco130I CCWWGG 7 cut(s) 816, 1663, 1757, 1840, 1944, 2078, 2445
Eco147I AGGCCT 1 cut(s) 915
Eco24I GRGCYC 5 cut(s) 232, 240, 391, 1062, 3143
Eco32I GATATC 1 cut(s) 2979
Eco53kI GAGCTC 2 cut(s) 238, 1060
Eco57I CTGAAG 4 cut(s) 80, 930, 1452, 1809
Eco72I CACGTG 1 cut(s) 118
Eco81I CCTNAGG 2 cut(s) 283, 963
EcoICRI GAGCTC 2 cut(s) 238, 1060
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoO109I RGGNCCY 3 cut(s) 228, 229, 387
EcoRI GAATTC 2 cut(s) 1795, 1895
EcoRII CCWGG 5 cut(s) 80, 231, 1571, 1769, 2473
EcoRV GATATC 1 cut(s) 2979
EcoT14I CCWWGG 7 cut(s) 816, 1663, 1757, 1840, 1944, 2078, 2445
EcoT38I GRGCYC 5 cut(s) 232, 240, 391, 1062, 3143
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 7 cut(s) 816, 1663, 1757, 1840, 1944, 2078, 2445
FalI AAGNNNNNCTT 6 cut(s) 539, 571, 1681, 1713, 2648, 2680
FauI CCCGC 5 cut(s) 153, 207, 1702, 2786, 3062
FbaI TGATCA 2 cut(s) 474, 1813
FblI GTMKAC 1 cut(s) 3082
FokI GGATG 4 cut(s) 503, 1622, 1803, 2807
FriOI GRGCYC 5 cut(s) 232, 240, 391, 1062, 3143
FspBI CTAG 6 cut(s) 1275, 2375, 2520, 2526, 2862, 3026
GlaI GCGC 3 cut(s) 5, 255, 298
GsaI CCCAGC 1 cut(s) 2861
GsuI CTGGAG 2 cut(s) 254, 1104
HaeII RGCGCY 2 cut(s) 257, 300
HaeIII GGCC 9 cut(s) 93, 122, 164, 230, 389, 915, 1554, 2424, 2876
HapII CCGG 2 cut(s) 525, 2715
HhaI GCGC 3 cut(s) 6, 256, 299
Hin1I GRCGYC 1 cut(s) 297
Hin6I GCGC 3 cut(s) 4, 254, 297
HinP1I GCGC 3 cut(s) 4, 254, 297
HincII GTYRAC 2 cut(s) 319, 1767
HindII GTYRAC 2 cut(s) 319, 1767
HindIII AAGCTT 2 cut(s) 2057, 2680
HpaII CCGG 2 cut(s) 525, 2715
HphI GGTGA 7 cut(s) 200, 624, 862, 902, 1145, 2462, 2729
Hpy166II GTNNAC 6 cut(s) 319, 632, 1475, 1767, 2457, 3083
Hpy8I GTNNAC 6 cut(s) 319, 632, 1475, 1767, 2457, 3083
HpyCH4III ACNGT 5 cut(s) 326, 863, 880, 1655, 2690
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 9 cut(s) 25, 225, 374, 414, 1512, 1660, 1835, 2038, 2744
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 297
HspAI GCGC 3 cut(s) 4, 254, 297
KasI GGCGCC 1 cut(s) 296
Ksp22I TGATCA 2 cut(s) 474, 1813
LmnI GCTCC 7 cut(s) 235, 270, 646, 785, 1497, 2129, 2572
LweI GCATC 3 cut(s) 1286, 1470, 1788
MaeI CTAG 6 cut(s) 1275, 2375, 2520, 2526, 2862, 3026
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 103, 2312, 2690
MfeI CAATTG 2 cut(s) 1128, 1719
MflI RGATCY 2 cut(s) 1789, 2219
MhlI GDGCHC 5 cut(s) 232, 240, 391, 1062, 3143
MlsI TGGCCA 1 cut(s) 1554
MluNI TGGCCA 1 cut(s) 1554
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 5 cut(s) 809, 1384, 2162, 2462, 2777
Mox20I TGGCCA 1 cut(s) 1554
MroXI GAANNNNTTC 3 cut(s) 2094, 2663, 2837
MscI TGGCCA 1 cut(s) 1554
MseI TTAA 6 cut(s) 258, 653, 786, 828, 2366, 3060
Msp20I TGGCCA 1 cut(s) 1554
MspA1I CMGCKG 2 cut(s) 41, 472
MspI CCGG 2 cut(s) 525, 2715
MspR9I CCNGG 6 cut(s) 82, 233, 526, 1573, 1771, 2475
MunI CAATTG 2 cut(s) 1128, 1719
Mva1269I GAATGC 1 cut(s) 1514
MvaI CCWGG 5 cut(s) 82, 233, 1573, 1771, 2475
MvnI CGCG 1 cut(s) 2781
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 526
NcoI CCATGG 1 cut(s) 1840
NmeAIII GCCGAG 1 cut(s) 1977
NmuCI GTSAC 2 cut(s) 103, 2312
NspI RCATGY 2 cut(s) 1010, 2053
NspV TTCGAA 1 cut(s) 2547
PceI AGGCCT 1 cut(s) 915
PciI ACATGT 2 cut(s) 1006, 2049
PcsI WCGNNNNNNNCGW 2 cut(s) 2544, 2826
PctI GAATGC 1 cut(s) 1514
PdmI GAANNNNTTC 3 cut(s) 2094, 2663, 2837
PleI GAGTC 5 cut(s) 808, 1383, 2162, 2461, 2777
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 5 cut(s) 808, 1383, 2162, 2461, 2777
Ppu21I YACGTR 1 cut(s) 118
PscI ACATGT 2 cut(s) 1006, 2049
PshBI ATTAAT 1 cut(s) 786
PsiI TTATAA 1 cut(s) 597
Psp124BI GAGCTC 2 cut(s) 240, 1062
Psp6I CCWGG 5 cut(s) 80, 231, 1571, 1769, 2473
PspCI CACGTG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 2857
PspGI CCWGG 5 cut(s) 80, 231, 1571, 1769, 2473
PspOMI GGGCCC 2 cut(s) 228, 387
PspPI GGNCC 5 cut(s) 162, 228, 229, 387, 388
PstNI CAGNNNCTG 2 cut(s) 803, 1832
PsuI RGATCY 2 cut(s) 1789, 2219
PvuII CAGCTG 1 cut(s) 472
SacI GAGCTC 2 cut(s) 240, 1062
SaqAI TTAA 6 cut(s) 258, 653, 786, 828, 2366, 3060
Sau96I GGNCC 5 cut(s) 162, 228, 229, 387, 388
ScaI AGTACT 3 cut(s) 538, 1744, 2518
SchI GAGTC 5 cut(s) 809, 1384, 2162, 2462, 2777
ScrFI CCNGG 6 cut(s) 82, 233, 526, 1573, 1771, 2475
SduI GDGCHC 5 cut(s) 232, 240, 391, 1062, 3143
SfaNI GCATC 3 cut(s) 1286, 1470, 1788
SfcI CTRYAG 2 cut(s) 795, 1651
SfoI GGCGCC 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 2547
SmlI CTYRAG 8 cut(s) 139, 154, 464, 532, 1237, 1319, 2193, 2668
SmoI CTYRAG 8 cut(s) 139, 154, 464, 532, 1237, 1319, 2193, 2668
SpeI ACTAGT 1 cut(s) 2519
SseBI AGGCCT 1 cut(s) 915
SspDI GGCGCC 1 cut(s) 296
SspI AATATT 1 cut(s) 2433
SspMI CTAG 6 cut(s) 1275, 2375, 2520, 2526, 2862, 3026
SstI GAGCTC 2 cut(s) 240, 1062
StuI AGGCCT 1 cut(s) 915
StyD4I CCNGG 6 cut(s) 80, 231, 524, 1571, 1769, 2473
StyI CCWWGG 7 cut(s) 816, 1663, 1757, 1840, 1944, 2078, 2445
TaaI ACNGT 5 cut(s) 326, 863, 880, 1655, 2690
TaiI ACGT 1 cut(s) 120
TatI WGTACW 5 cut(s) 536, 1701, 1742, 1886, 2516
TauI GCSGC 4 cut(s) 125, 182, 297, 302
Tru1I TTAA 6 cut(s) 258, 653, 786, 828, 2366, 3060
Tru9I TTAA 6 cut(s) 258, 653, 786, 828, 2366, 3060
TscAI CASTG 6 cut(s) 596, 610, 883, 1396, 1969, 2317
TseFI GTSAC 2 cut(s) 103, 2312
Tsp45I GTSAC 2 cut(s) 103, 2312
TspGWI ACGGA 1 cut(s) 2807
TspRI CASTG 6 cut(s) 596, 610, 883, 1396, 1969, 2317
VspI ATTAAT 1 cut(s) 786
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 8 cut(s) 682, 992, 1197, 1795, 1895, 2369, 3062, 3165
XceI RCATGY 2 cut(s) 1010, 2053
XcmI CCANNNNNNNNNTGG 2 cut(s) 310, 2807
XmiI GTMKAC 1 cut(s) 3082
XmnI GAANNNNTTC 3 cut(s) 2094, 2663, 2837
XspI CTAG 6 cut(s) 1275, 2375, 2520, 2526, 2862, 3026
ZrmI AGTACT 3 cut(s) 538, 1744, 2518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.