MD17G1059900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013173
Physical Location & Seq
Forward (+)
4864016 .. 4864873
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1059900.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGATGATGCATATAGGGATGCAAGTGGTAGTAAGCATCCAAGGGTGCATTTTGAAAGCGATCTGGTTAATCACCACCGAAATTCAAGCTTCCCATCATCCTCGTCATCATCACCATCGTCCTCATCATCGTCATCTCCACGTTCATCGCTGGAATCATCTGTCAATGGTACAGAAGGTGGTAACAATCCCTGCGTAACAACGACACGACCAGACCACGAGTCTCAGGGGGCTCTGTCATTACCAGAGGGTTATTCTTCTCCGATTCTGCCTTGGAGCATGCAAAGCGGATCATCCGCGCAATCCTCTCAGTTTCAGATGATGGGAAGGCCTGCAGGATATGACCCGAGTCGAATCCCATCGAATATTTTTTCCAGTAAACCGGCAGCAGGCATGGATTGGAGTGTTGCTTCAAATGAATCATTATTTAGTCTTCATGTGGGGAACAACAGCTTCTCGAGAGATCAGTTTGCTTTTCTGAATAAATCCGGAGAGCTTTATAAATCCGGTGAACTGACGCGGCATGATGAATTATTCTACATTCCAACTCCACTTCCCACAGTTACTGAGGCAGAAACTATTGAAATTAAGATTCACAATGTGGAGAAAGAGAAGGTTGAAAACAAGGCTCCGAACGTGGAGGCAAAAATCGAGGAAAATGAGAGTGCAAATGTGGAGATATATAAGGTTGAAAGCGTGAGGGAAAATGTGGAAAAGGAATCAGTAGAAGAAACAGAAGGACCAGATGAATCCGCAAAGACAGAAGCCATAAAACCCAAGGAAATCTGGAATTCTGTCGCTTCATACCGGTCTGATGGGAGCAATAACAGCACCAAGTCATTCCGGTTCCCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.21

Weight (kDa)

5.06

Isoelectric Point (pI)

60.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39140 AT5G39170 AT5G39200
fragaria_vesca FvH4_6g46831
malus_domestica MD09G1067000.v1.1 MD17G1059900.v1.1
prunus_persica Prupe.3G254700_v2.0.a1
pyrus_communis pycom111g05630 pycom17g05960
rosa_laevigata RLG00000021559
rosa_multiflora Rmu_sc0003227.1_g000003
rosa_roxburghii Rroxscaffold_2G00085470
rosa_rugosa Rorug02G0518100
rosa_samantha Rh2AG583800 Rh2BG595800 Rh2CG566700 Rh2DG605600
rosa_wichuraiana Rw2G048680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccII CGCG 2 cut(s) 299, 520
AccIII TCCGGA 1 cut(s) 488
AciI CCGC 4 cut(s) 288, 297, 520, 753
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 2 cut(s) 82, 790
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 389
AgeI ACCGGT 1 cut(s) 807
AgsI TTSAA 6 cut(s) 56, 87, 414, 584, 620, 692
AhdI GACNNNNNGTC 1 cut(s) 220
AluBI AGCT 3 cut(s) 90, 453, 496
AluI AGCT 3 cut(s) 90, 453, 496
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 298
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 346, 457
Aor13HI TCCGGA 1 cut(s) 488
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 2 cut(s) 82, 790
AsiGI ACCGGT 1 cut(s) 807
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 1 cut(s) 740
AsuHPI GGTGA 3 cut(s) 65, 105, 521
AvaI CYCGRG 2 cut(s) 346, 457
AvaII GGWCC 1 cut(s) 740
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 398
BccI CCATC 5 cut(s) 103, 124, 316, 367, 809
BcoDI GTCTC 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 2 cut(s) 387, 521
BlsI GCNGC 2 cut(s) 388, 522
Bme18I GGWCC 1 cut(s) 740
BmeRI GACNNNNNGTC 1 cut(s) 220
BmeT110I CYCGRG 2 cut(s) 346, 457
BmgT120I GGNCC 1 cut(s) 740
BmiI GGNNCC 2 cut(s) 630, 848
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 2 cut(s) 10, 46
BmuI ACTGGG 1 cut(s) 845
BoxI GACNNNNGTC 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 425
BsaJI CCNNGG 3 cut(s) 41, 272, 777
BsaWI WCCGGW 4 cut(s) 488, 506, 807, 843
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse118I RCCGGY 2 cut(s) 382, 807
Bse1I ACTGG 2 cut(s) 375, 851
BseAI TCCGGA 1 cut(s) 488
BseDI CCNNGG 3 cut(s) 41, 272, 777
BseGI GGATG 5 cut(s) 10, 25, 37, 98, 293
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 3 cut(s) 239, 323, 558
BseNI ACTGG 2 cut(s) 375, 851
BseXI GCAGC 1 cut(s) 398
Bsh1236I CGCG 2 cut(s) 299, 520
BshFI GGCC 1 cut(s) 331
BshTI ACCGGT 1 cut(s) 807
BsiHKCI CYCGRG 2 cut(s) 346, 457
BsiSI CCGG 5 cut(s) 383, 489, 507, 808, 844
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 2 cut(s) 346, 457
Bsp1286I GDGCHC 1 cut(s) 235
Bsp13I TCCGGA 1 cut(s) 488
Bsp143I GATC 3 cut(s) 61, 290, 463
BspACI CCGC 4 cut(s) 288, 297, 520, 753
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 3 cut(s) 238, 322, 559
BspEI TCCGGA 1 cut(s) 488
BspFNI CGCG 2 cut(s) 299, 520
BspLI GGNNCC 2 cut(s) 630, 848
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 298
BsrFI RCCGGY 2 cut(s) 382, 807
BsrI ACTGG 2 cut(s) 375, 851
BssAI RCCGGY 2 cut(s) 382, 807
BssECI CCNNGG 3 cut(s) 41, 272, 777
BssMI GATC 3 cut(s) 61, 290, 463
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 3 cut(s) 41, 272, 777
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 562
BstC8I GCNNGC 3 cut(s) 281, 333, 391
BstDEI CTNAG 3 cut(s) 225, 309, 567
BstF5I GGATG 5 cut(s) 10, 25, 37, 98, 293
BstFNI CGCG 2 cut(s) 299, 520
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 3 cut(s) 64, 293, 466
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 3 cut(s) 61, 290, 463
BstMWI GCNNNNNNNGC 2 cut(s) 285, 828
BstNSI RCATGY 1 cut(s) 283
BstPAI GACNNNNGTC 1 cut(s) 348
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 2 cut(s) 299, 520
BstV1I GCAGC 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 425
BsuRI GGCC 1 cut(s) 331
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 5 cut(s) 10, 25, 37, 98, 293
BtsIMutI CAGTG 1 cut(s) 858
Cac8I GCNNGC 3 cut(s) 281, 333, 391
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 301
Cfr10I RCCGGY 2 cut(s) 382, 807
Cfr13I GGNCC 1 cut(s) 740
CseI GACGC 1 cut(s) 526
Csp6I GTAC 1 cut(s) 171
CspAI ACCGGT 1 cut(s) 807
CviAII CATG 4 cut(s) 280, 394, 437, 524
CviJI RGCY 7 cut(s) 90, 233, 331, 453, 496, 629, 767
CviKI_1 RGCY 7 cut(s) 90, 233, 331, 453, 496, 629, 767
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 3 cut(s) 225, 309, 567
DpnI GATC 3 cut(s) 63, 292, 465
DpnII GATC 3 cut(s) 61, 290, 463
DraIII CACNNNGTG 1 cut(s) 601
DriI GACNNNNNGTC 1 cut(s) 220
Eam1105I GACNNNNNGTC 1 cut(s) 220
Eco130I CCWWGG 3 cut(s) 41, 272, 777
Eco147I AGGCCT 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 740
Eco88I CYCGRG 2 cut(s) 346, 457
EcoRI GAATTC 1 cut(s) 790
EcoT14I CCWWGG 3 cut(s) 41, 272, 777
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 3 cut(s) 41, 272, 777
FaeI CATG 4 cut(s) 283, 397, 440, 527
FatI CATG 4 cut(s) 279, 393, 436, 523
Fnu4HI GCNGC 2 cut(s) 387, 521
FokI GGATG 5 cut(s) 17, 24, 32, 85, 280
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 387, 521
GlaI GCGC 1 cut(s) 300
GluI GCNGC 2 cut(s) 387, 521
HaeIII GGCC 1 cut(s) 331
HapII CCGG 5 cut(s) 383, 489, 507, 808, 844
HgaI GACGC 1 cut(s) 526
HhaI GCGC 1 cut(s) 301
Hin1II CATG 4 cut(s) 283, 397, 440, 527
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 9 cut(s) 155, 221, 265, 349, 354, 419, 592, 719, 749
HpaII CCGG 5 cut(s) 383, 489, 507, 808, 844
HphI GGTGA 3 cut(s) 65, 105, 521
Hpy166II GTNNAC 2 cut(s) 380, 512
Hpy188I TCNGA 5 cut(s) 264, 318, 480, 633, 814
Hpy188III TCNNGA 4 cut(s) 457, 459, 489, 787
Hpy8I GTNNAC 2 cut(s) 380, 512
HpyAV CCTTC 4 cut(s) 170, 321, 607, 731
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 2 cut(s) 142, 636
HpyCH4V TGCA 6 cut(s) 11, 23, 49, 283, 335, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 828
HpyF3I CTNAG 3 cut(s) 225, 309, 567
HpySE526I ACGT 2 cut(s) 142, 636
Hsp92II CATG 4 cut(s) 283, 397, 440, 527
HspAI GCGC 1 cut(s) 299
Kpn2I TCCGGA 1 cut(s) 488
Kzo9I GATC 3 cut(s) 61, 290, 463
LmnI GCTCC 3 cut(s) 276, 634, 819
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 2 cut(s) 10, 46
MaeII ACGT 2 cut(s) 142, 636
MaeIII GTNAC 3 cut(s) 182, 196, 562
MalI GATC 3 cut(s) 63, 292, 465
MboI GATC 3 cut(s) 61, 290, 463
MboII GAAGA 3 cut(s) 249, 425, 740
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 4 cut(s) 82, 530, 585, 790
MlyI GAGTC 2 cut(s) 230, 358
MmeI TCCRAC 1 cut(s) 569
MnlI CCTC 8 cut(s) 112, 133, 241, 316, 562, 634, 646, 693
Mph1103I ATGCAT 1 cut(s) 13
MroI TCCGGA 1 cut(s) 488
MseI TTAA 2 cut(s) 69, 588
MspI CCGG 5 cut(s) 383, 489, 507, 808, 844
MvnI CGCG 2 cut(s) 299, 520
MwoI GCNNNNNNNGC 2 cut(s) 285, 828
NdeII GATC 3 cut(s) 61, 290, 463
NlaIII CATG 4 cut(s) 283, 397, 440, 527
NlaIV GGNNCC 2 cut(s) 630, 848
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 457
PceI AGGCCT 1 cut(s) 331
PfeI GAWTC 7 cut(s) 155, 265, 354, 419, 592, 719, 749
PinAI ACCGGT 1 cut(s) 807
PkrI GCNGC 2 cut(s) 388, 522
PleI GAGTC 2 cut(s) 229, 357
PpsI GAGTC 2 cut(s) 229, 357
PshAI GACNNNNGTC 1 cut(s) 348
PsiI TTATAA 1 cut(s) 501
PspN4I GGNNCC 2 cut(s) 630, 848
PspPI GGNCC 1 cut(s) 740
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 566
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
SaqAI TTAA 2 cut(s) 69, 588
SatI GCNGC 2 cut(s) 387, 521
Sau3AI GATC 3 cut(s) 61, 290, 463
Sau96I GGNCC 1 cut(s) 740
SbfI CCTGCAGG 1 cut(s) 337
SchI GAGTC 2 cut(s) 230, 358
SdaI CCTGCAGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 235
SetI ASST 8 cut(s) 92, 145, 181, 455, 498, 618, 639, 690
SfaNI GCATC 2 cut(s) 10, 46
SfcI CTRYAG 1 cut(s) 333
Sfr274I CTCGAG 1 cut(s) 457
SinI GGWCC 1 cut(s) 740
SlaI CTCGAG 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
SphI GCATGC 1 cut(s) 283
Sse8387I CCTGCAGG 1 cut(s) 337
Sse9I AATT 4 cut(s) 82, 530, 585, 790
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 4 cut(s) 288, 297, 520, 753
SspI AATATT 1 cut(s) 367
StuI AGGCCT 1 cut(s) 331
StyI CCWWGG 3 cut(s) 41, 272, 777
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 2 cut(s) 145, 639
TaqI TCGA 4 cut(s) 352, 362, 458, 651
TasI AATT 4 cut(s) 82, 530, 585, 790
TauI GCSGC 1 cut(s) 523
TfiI GAWTC 7 cut(s) 155, 265, 354, 419, 592, 719, 749
Tru1I TTAA 2 cut(s) 69, 588
Tru9I TTAA 2 cut(s) 69, 588
TscAI CASTG 1 cut(s) 858
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 6 cut(s) 135, 425, 432, 543, 762, 792
TspRI CASTG 1 cut(s) 858
VpaK11BI GGWCC 1 cut(s) 740
XapI RAATTY 2 cut(s) 82, 790
XceI RCATGY 1 cut(s) 283
XhoI CTCGAG 1 cut(s) 457
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.