pycom111g05630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0013173
Physical Location & Seq
Forward (+)
3595526 .. 3596911
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g05630.2

Sequence Viewer

Length: 1077 bp
ATGGATGATGCATATAGGGATGAATGTGGTAATAAGCATCTAAGGGTGAATTTTGAAAGCGATGCGGTTAATTACCGCCGAAATTCAAGCTGCCCATCATCCTCGTCATCATCACCATCATCATCATCATCGTCATCTCCACGTTCATCGCTGGAATCATTTGTCAATGACAAAAAAGATGGCAACAATCCATGCGTAACAACGACACGACCAGACGAGGAGTCTCAGGGGGCTCTATCATTACCAGAGGGTTATTCTTCTCCGAGTCTGCCTGGGAGCATGCAAAGCGGATCATCTGCGCAATCTTCTCCGTTTCAGATGATGGGAAGGCCTGCAGGATATGACCCAAGTCGAATCCCATCGAATATTTTCGCCAGTAAACCGCCAGCAGGCATGGATTGGAGTGTTGCTTCAAATGAATCATTGTTTAGTCTTCATGTGGGGAACAACAGTTTCTCGAGAGATCAATTCGCTTTGCTGAATAAATCCGGAGAGCTTTACAAATCCGGAGAGCTTTATAAATCCGGTGAACTGACGAGGCATGATGAATACATCTACATTCCAACTCCACTTCCCACAGTTACTGAGGTAGAAACTATTGAAATAAAGATTCACAATGTGGAGAAAGAGAAGGTTGAAAACAAGGCTCCGAACGTGGAGGCGAAAATCGAAGAAAATGAGAGTGCAAATGTGGAATATAAGGTTGAAAGCATGAGGGAAAATGTGGAAACGGAATCAGTAGAAGAAACAGAAGGACCAGATGAATCTGCAAAGACAGAAGCCGTCAAAAAGGAAAAGGTGCCTCTTATCGGAGAAATTCAGAATCCTGCTGCTTCATACCAATCTGATGGGAGCAATAACAGCACCAAGTCATTCCAGTTTCCAGTGTTGGCAAGTCATGAGGTTGAAAGCAATATCCCTGCAGAAATGAACTCAGGAAAGCTACAGTCAGCGCACGCACAGCAGGAGATGCATAAGCAAGTAGGGACTGAAGCCCCCGAAACACCCCTAAAGGCAGCCACAACTCAAATAACTAGTTGGTTTTCGTGCTTCTGTTGTTGCACCGCCCGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

359

Amino Acids

39.12

Weight (kDa)

4.91

Isoelectric Point (pI)

66.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39140 AT5G39170 AT5G39200
fragaria_vesca FvH4_6g46831
malus_domestica MD09G1067000.v1.1 MD17G1059900.v1.1
prunus_persica Prupe.3G254700_v2.0.a1
pyrus_communis pycom111g05630 pycom17g05960
rosa_laevigata RLG00000021559
rosa_multiflora Rmu_sc0003227.1_g000003
rosa_roxburghii Rroxscaffold_2G00085470
rosa_rugosa Rorug02G0518100
rosa_samantha Rh2AG583800 Rh2BG595800 Rh2CG566700 Rh2DG605600
rosa_wichuraiana Rw2G048680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
Acc16I TGCGCA 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 799
AccIII TCCGGA 2 cut(s) 488, 506
AciI CCGC 6 cut(s) 65, 76, 288, 383, 1065, 1069
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 3 cut(s) 49, 82, 816
AcuI CTGAAG 1 cut(s) 1011
AdeI CACNNNGTG 1 cut(s) 619
AfiI CCNNNNNNNGG 2 cut(s) 389, 809
AgsI TTSAA 7 cut(s) 56, 87, 414, 602, 638, 707, 908
AhdI GACNNNNNGTC 1 cut(s) 220
AhlI ACTAGT 1 cut(s) 1034
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 4 cut(s) 90, 496, 514, 943
AluI AGCT 4 cut(s) 90, 496, 514, 943
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 298
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 457
Aor13HI TCCGGA 2 cut(s) 488, 506
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 3 cut(s) 90, 830, 1016
ApoI RAATTY 3 cut(s) 49, 82, 816
Asp700I GAANNNNTTC 1 cut(s) 368
AspLEI GCGC 2 cut(s) 301, 955
AspS9I GGNCC 1 cut(s) 755
AsuHPI GGTGA 3 cut(s) 58, 105, 539
AvaI CYCGRG 1 cut(s) 457
AvaII GGWCC 1 cut(s) 755
BanI GGYRCC 1 cut(s) 799
BanII GRGCYC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 3 cut(s) 77, 817, 1028
BccI CCATC 6 cut(s) 103, 124, 173, 316, 367, 842
BceAI ACGGC 2 cut(s) 767, 1056
BciT130I CCWGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 228
BcuI ACTAGT 1 cut(s) 1034
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 3 cut(s) 333, 921, 944
BisI GCNGC 3 cut(s) 91, 831, 1017
BlsI GCNGC 3 cut(s) 92, 832, 1018
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 1 cut(s) 755
BmeRI GACNNNNNGTC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 2 cut(s) 648, 801
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 3 cut(s) 46, 52, 960
BoxI GACNNNNGTC 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 272
BsaWI WCCGGW 3 cut(s) 488, 506, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 809
Bse1I ACTGG 3 cut(s) 375, 877, 884
BseAI TCCGGA 2 cut(s) 488, 506
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 3 cut(s) 10, 25, 98
BseLI CCNNNNNNNGG 2 cut(s) 389, 809
BseMII CTCAG 3 cut(s) 239, 576, 948
BseNI ACTGG 3 cut(s) 375, 877, 884
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 3 cut(s) 77, 817, 1028
BshFI GGCC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 799
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 3 cut(s) 489, 507, 525
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 2 cut(s) 389, 809
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 1000
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 235
Bsp13I TCCGGA 2 cut(s) 488, 506
Bsp143I GATC 2 cut(s) 290, 463
BspACI CCGC 6 cut(s) 65, 76, 288, 383, 1065, 1069
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 3 cut(s) 238, 577, 947
BspEI TCCGGA 2 cut(s) 488, 506
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 2 cut(s) 648, 801
BspMAI CTGCAG 2 cut(s) 337, 925
BspPI GGATC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 799
BsrI ACTGG 3 cut(s) 375, 877, 884
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 2 cut(s) 290, 463
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 3 cut(s) 452, 580, 948
BstAPI GCANNNNNTGC 1 cut(s) 970
BstC8I GCNNGC 6 cut(s) 281, 333, 387, 391, 957, 1069
BstDEI CTNAG 4 cut(s) 41, 225, 585, 934
BstF5I GGATG 3 cut(s) 10, 25, 98
BstHHI GCGC 2 cut(s) 301, 955
BstKTI GATC 2 cut(s) 293, 466
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 2 cut(s) 290, 463
BstMWI GCNNNNNNNGC 5 cut(s) 285, 861, 961, 970, 1068
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 1 cut(s) 283
BstPAI GACNNNNGTC 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 3 cut(s) 333, 921, 944
BstV1I GCAGC 3 cut(s) 77, 817, 1028
BstV2I GAAGAC 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 848
BsuRI GGCC 1 cut(s) 331
BtgZI GCGATG 2 cut(s) 75, 132
BtsCI GGATG 3 cut(s) 10, 25, 98
BtsIMutI CAGTG 1 cut(s) 891
Cac8I GCNNGC 6 cut(s) 281, 333, 387, 391, 957, 1069
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 898
CfoI GCGC 2 cut(s) 301, 955
Cfr13I GGNCC 1 cut(s) 755
CviAII CATG 7 cut(s) 192, 280, 394, 437, 542, 712, 899
DdeI CTNAG 4 cut(s) 41, 225, 585, 934
DpnI GATC 2 cut(s) 292, 465
DpnII GATC 2 cut(s) 290, 463
DraIII CACNNNGTG 1 cut(s) 619
DriI GACNNNNNGTC 1 cut(s) 220
Eam1105I GACNNNNNGTC 1 cut(s) 220
Eco147I AGGCCT 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 755
Eco57I CTGAAG 1 cut(s) 1011
Eco88I CYCGRG 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 271
EcoT22I ATGCAT 2 cut(s) 13, 975
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 7 cut(s) 195, 283, 397, 440, 545, 715, 902
FaqI GGGAC 1 cut(s) 1000
FatI CATG 7 cut(s) 191, 279, 393, 436, 541, 711, 898
Fnu4HI GCNGC 3 cut(s) 91, 831, 1017
FokI GGATG 3 cut(s) 17, 32, 85
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 3 cut(s) 91, 831, 1017
FspBI CTAG 1 cut(s) 1035
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 2 cut(s) 300, 954
GluI GCNGC 3 cut(s) 91, 831, 1017
HaeIII GGCC 1 cut(s) 331
HapII CCGG 3 cut(s) 489, 507, 525
HhaI GCGC 2 cut(s) 301, 955
Hin1II CATG 7 cut(s) 195, 283, 397, 440, 545, 715, 902
Hin6I GCGC 2 cut(s) 299, 953
HinP1I GCGC 2 cut(s) 299, 953
HinfI GANTC 9 cut(s) 155, 221, 265, 354, 419, 610, 734, 764, 823
HpaII CCGG 3 cut(s) 489, 507, 525
HphI GGTGA 3 cut(s) 58, 105, 539
Hpy166II GTNNAC 2 cut(s) 380, 530
Hpy188I TCNGA 6 cut(s) 264, 318, 651, 812, 822, 847
Hpy188III TCNNGA 6 cut(s) 457, 459, 489, 507, 899, 936
Hpy8I GTNNAC 2 cut(s) 380, 530
HpyAV CCTTC 3 cut(s) 321, 625, 746
HpyCH4III ACNGT 3 cut(s) 452, 580, 948
HpyCH4IV ACGT 2 cut(s) 142, 654
HpyCH4V TGCA 8 cut(s) 11, 283, 335, 686, 770, 923, 973, 1062
HpyF10VI GCNNNNNNNGC 5 cut(s) 285, 861, 961, 970, 1068
HpyF3I CTNAG 4 cut(s) 41, 225, 585, 934
HpySE526I ACGT 2 cut(s) 142, 654
Hsp92II CATG 7 cut(s) 195, 283, 397, 440, 545, 715, 902
HspAI GCGC 2 cut(s) 299, 953
Kpn2I TCCGGA 2 cut(s) 488, 506
Kzo9I GATC 2 cut(s) 290, 463
LmnI GCTCC 3 cut(s) 276, 652, 852
Lsp1109I GCAGC 3 cut(s) 77, 817, 1028
LweI GCATC 3 cut(s) 46, 52, 960
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 2 cut(s) 142, 654
MaeIII GTNAC 2 cut(s) 196, 580
MalI GATC 2 cut(s) 292, 465
MboI GATC 2 cut(s) 290, 463
MboII GAAGA 5 cut(s) 249, 297, 425, 683, 755
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 5 cut(s) 49, 70, 82, 467, 816
MlyI GAGTC 2 cut(s) 230, 274
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 9 cut(s) 112, 211, 241, 531, 580, 652, 708, 813, 895
Mph1103I ATGCAT 2 cut(s) 13, 975
MroI TCCGGA 2 cut(s) 488, 506
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 1 cut(s) 69
MspI CCGG 3 cut(s) 489, 507, 525
MspR9I CCNGG 1 cut(s) 273
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 5 cut(s) 285, 861, 961, 970, 1068
NdeII GATC 2 cut(s) 290, 463
NlaIII CATG 7 cut(s) 195, 283, 397, 440, 545, 715, 902
NlaIV GGNNCC 2 cut(s) 648, 801
NsbI TGCGCA 1 cut(s) 300
NsiI ATGCAT 2 cut(s) 13, 975
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 457
PagI TCATGA 1 cut(s) 898
PceI AGGCCT 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 7 cut(s) 155, 354, 419, 610, 734, 764, 823
PkrI GCNGC 3 cut(s) 92, 832, 1018
PleI GAGTC 2 cut(s) 229, 273
PpsI GAGTC 2 cut(s) 229, 273
PshAI GACNNNNGTC 1 cut(s) 348
PsiI TTATAA 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 2 cut(s) 648, 801
PspPI GGNCC 1 cut(s) 755
PstI CTGCAG 2 cut(s) 337, 925
PstNI CAGNNNCTG 1 cut(s) 584
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 3 cut(s) 91, 831, 1017
Sau3AI GATC 2 cut(s) 290, 463
Sau96I GGNCC 1 cut(s) 755
SbfI CCTGCAGG 1 cut(s) 337
SchI GAGTC 2 cut(s) 230, 274
ScrFI CCNGG 1 cut(s) 273
SdaI CCTGCAGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 3 cut(s) 46, 52, 960
SfcI CTRYAG 3 cut(s) 333, 921, 944
Sfr274I CTCGAG 1 cut(s) 457
SinI GGWCC 1 cut(s) 755
SlaI CTCGAG 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
SpeI ACTAGT 1 cut(s) 1034
SphI GCATGC 1 cut(s) 283
Sse8387I CCTGCAGG 1 cut(s) 337
Sse9I AATT 5 cut(s) 49, 70, 82, 467, 816
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 6 cut(s) 65, 76, 288, 383, 1065, 1069
SspI AATATT 1 cut(s) 367
SspMI CTAG 1 cut(s) 1035
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 271
TaaI ACNGT 3 cut(s) 452, 580, 948
TaiI ACGT 2 cut(s) 145, 657
TaqI TCGA 4 cut(s) 352, 362, 458, 669
TasI AATT 5 cut(s) 49, 70, 82, 467, 816
TfiI GAWTC 7 cut(s) 155, 354, 419, 610, 734, 764, 823
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 891
TseI GCWGC 3 cut(s) 90, 830, 1016
TspDTI ATGAA 8 cut(s) 36, 135, 425, 432, 561, 777, 825, 944
TspGWI ACGGA 2 cut(s) 300, 746
TspRI CASTG 1 cut(s) 891
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 3 cut(s) 49, 82, 816
XceI RCATGY 1 cut(s) 283
XhoI CTCGAG 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 1 cut(s) 1035
Zsp2I ATGCAT 2 cut(s) 13, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.