MD17G1106400.v1.1

Tyrosine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009937
Physical Location & Seq
Reverse (-)
9088360 .. 9091850
3491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1106400.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAAACTGGAGCTCAATAATCTTCATCATCCGGAGGATCAAGAGACCGACAGCGAGAGCGAGATGTGCAGGACAATCGAAGTTGTCGCGGGAAGTGGAGGCGTCCAGCTCTCTCCGGATAAGCAGACCTCCGGCGCCGACGACTGCGCCGACGACCTCTTCATTCCGCCGCTCAACTTCTCCTTGGTCGATAACGGCATTTTCAGGTCGGGCTTCCCGGAGCCCGCCAACTTCTCCTTTCTCCACACTCTAGGCCTCCGCTCCATCATATGTTTGTGTCCGGAGCCGTATCCAGAGCCGAATACGGAGTTTTTAAAGTCCAACGGGATTAAGCTTTTTCAGTTTGGAATTGAAGGCTACAAGGAGCCTTTTGTAAACATCCCGGATGATACAATCCGTGAAGCACTAAAAGTTGTCCTCGATGTAAGGAACCACCCTGTCTTAATTCATTGTAAACGAGGGAAGCATCGAACGGGTTGTCTGGTGGGGTGCTTGAGAAAATTGCAGAGATGGTGCCTCACGTCTGTTTTTGACGAATACCAGCGGTTTGCAGCTGCGAAAGCGAGAGTTGCGGATCAGAGGTTTATGGAGATGTTTGATGTTTCCAGTTTGAAACATCTGCCGATGACATTTTCATGTTCCAAGAGGTAA

Protein Analysis

217

Amino Acids

24.42

Weight (kDa)

5.98

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Y_phosphatase2 PF03162 55 - 206 4.3e-64 Tyrosine phosphatase family
PTP-SAK PF22784 79 - 166 1.3e-06 Swiss Army Knife protein, DSP-PTPase phosphatase domain
PTP-NADK PF22741 82 - 173 1.4e-07 DSP-PTPase phosphatase fused to NAD+ Kinase
Y_phosphatase3 PF13350 126 - 186 1.1e-06 Tyrosine phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 134, 513
AccBSI CCGCTC 2 cut(s) 172, 261
AccII CGCG 1 cut(s) 89
AccIII TCCGGA 3 cut(s) 31, 115, 280
AciI CCGC 7 cut(s) 89, 167, 170, 225, 259, 544, 572
AclWI GGATC 2 cut(s) 45, 582
AcyI GRCGYC 2 cut(s) 102, 135
AgsI TTSAA 2 cut(s) 353, 613
AjiI CACGTC 1 cut(s) 522
AluBI AGCT 4 cut(s) 13, 109, 334, 554
AluI AGCT 4 cut(s) 13, 109, 334, 554
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 2 cut(s) 45, 582
Aor13HI TCCGGA 3 cut(s) 31, 115, 280
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 551, 554
ArsI GACNNNNNNTTYG 2 cut(s) 463, 495
AspLEI GCGC 2 cut(s) 137, 149
AsuC2I CCSGG 2 cut(s) 218, 383
BanI GGYRCC 2 cut(s) 134, 513
BanII GRGCYC 2 cut(s) 15, 225
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 541, 563
BccI CCATC 2 cut(s) 272, 504
BceAI ACGGC 2 cut(s) 211, 271
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciVI GTATCC 1 cut(s) 300
BcnI CCSGG 2 cut(s) 218, 383
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 138
BfuI GTATCC 1 cut(s) 300
BisI GCNGC 3 cut(s) 170, 552, 555
BlsI GCNGC 3 cut(s) 171, 553, 556
Bme1390I CCNGG 2 cut(s) 218, 383
BmgBI CACGTC 1 cut(s) 522
BmiI GGNNCC 6 cut(s) 136, 222, 285, 366, 431, 515
BmrFI CCNGG 2 cut(s) 218, 383
BmsI GCATC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 514
BpuMI CCSGG 2 cut(s) 218, 383
BsaHI GRCGYC 2 cut(s) 102, 135
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 183
BsaWI WCCGGW 3 cut(s) 31, 115, 280
Bse1I ACTGG 2 cut(s) 12, 606
BseAI TCCGGA 3 cut(s) 31, 115, 280
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 3 cut(s) 28, 378, 391
BseNI ACTGG 2 cut(s) 12, 606
BseXI GCAGC 2 cut(s) 541, 563
BsgI GTGCAG 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 2 cut(s) 134, 513
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 6 cut(s) 32, 116, 132, 218, 281, 383
BsmAI GTCTC 1 cut(s) 38
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 15, 225
Bsp13I TCCGGA 3 cut(s) 31, 115, 280
Bsp143I GATC 2 cut(s) 37, 574
BspACI CCGC 7 cut(s) 89, 167, 170, 225, 259, 544, 572
BspANI GGCC 1 cut(s) 255
BspEI TCCGGA 3 cut(s) 31, 115, 280
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 6 cut(s) 136, 222, 285, 366, 431, 515
BspPI GGATC 2 cut(s) 45, 582
BspT107I GGYRCC 2 cut(s) 134, 513
BspTNI GGTCTC 1 cut(s) 38
BsrBI CCGCTC 2 cut(s) 172, 261
BsrI ACTGG 2 cut(s) 12, 606
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 2 cut(s) 37, 574
BssNI GRCGYC 2 cut(s) 102, 135
BssT1I CCWWGG 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 2 cut(s) 102, 135
BstC8I GCNNGC 1 cut(s) 225
BstF5I GGATG 3 cut(s) 28, 378, 391
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 149
BstKTI GATC 2 cut(s) 40, 577
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 2 cut(s) 37, 574
BstMWI GCNNNNNNNGC 3 cut(s) 66, 560, 569
BstSCI CCNGG 2 cut(s) 216, 381
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 541, 563
BsuI GTATCC 1 cut(s) 300
BsuRI GGCC 1 cut(s) 255
BtrI CACGTC 1 cut(s) 522
BtsCI GGATG 3 cut(s) 28, 378, 391
Cac8I GCNNGC 1 cut(s) 225
CfoI GCGC 2 cut(s) 137, 149
CseI GACGC 1 cut(s) 91
CviAII CATG 1 cut(s) 636
DinI GGCGCC 1 cut(s) 136
DpnI GATC 2 cut(s) 39, 576
DpnII GATC 2 cut(s) 37, 574
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 255
Eco24I GRGCYC 2 cut(s) 15, 225
Eco31I GGTCTC 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 2 cut(s) 15, 225
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 1 cut(s) 639
FaiI YATR 4 cut(s) 269, 271, 587, 637
FatI CATG 1 cut(s) 635
FauI CCCGC 2 cut(s) 82, 232
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 170, 552, 555
FokI GGATG 3 cut(s) 15, 365, 398
FriOI GRGCYC 2 cut(s) 15, 225
Fsp4HI GCNGC 3 cut(s) 170, 552, 555
FspBI CTAG 1 cut(s) 251
GlaI GCGC 2 cut(s) 136, 148
GluI GCNGC 3 cut(s) 170, 552, 555
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 1 cut(s) 255
HapII CCGG 6 cut(s) 32, 116, 132, 218, 281, 383
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 137, 149
Hin1I GRCGYC 2 cut(s) 102, 135
Hin1II CATG 1 cut(s) 639
Hin6I GCGC 2 cut(s) 135, 147
HinP1I GCGC 2 cut(s) 135, 147
HindIII AAGCTT 1 cut(s) 332
HpaII CCGG 6 cut(s) 32, 116, 132, 218, 281, 383
Hpy166II GTNNAC 2 cut(s) 376, 455
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 5 cut(s) 32, 41, 116, 281, 293
Hpy8I GTNNAC 2 cut(s) 376, 455
Hpy99I CGWCG 2 cut(s) 143, 155
HpyAV CCTTC 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 521
HpyCH4V TGCA 3 cut(s) 69, 505, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 560, 569
HpySE526I ACGT 1 cut(s) 521
Hsp92I GRCGYC 2 cut(s) 102, 135
Hsp92II CATG 1 cut(s) 639
HspAI GCGC 2 cut(s) 135, 147
KasI GGCGCC 1 cut(s) 134
Kpn2I TCCGGA 3 cut(s) 31, 115, 280
Kzo9I GATC 2 cut(s) 37, 574
LmnI GCTCC 5 cut(s) 10, 220, 266, 283, 364
Lsp1109I GCAGC 2 cut(s) 541, 563
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 521
MalI GATC 2 cut(s) 39, 576
MbiI CCGCTC 2 cut(s) 172, 261
MboI GATC 2 cut(s) 37, 574
MboII GAAGA 2 cut(s) 14, 151
MhlI GDGCHC 2 cut(s) 15, 225
MluCI AATT 3 cut(s) 348, 444, 500
Mly113I GGCGCC 1 cut(s) 135
MmeI TCCRAC 1 cut(s) 345
MroI TCCGGA 3 cut(s) 31, 115, 280
MseI TTAA 3 cut(s) 314, 330, 443
MslI CAYNNNNRTG 1 cut(s) 634
MspA1I CMGCKG 2 cut(s) 544, 554
MspI CCGG 6 cut(s) 32, 116, 132, 218, 281, 383
MspR9I CCNGG 2 cut(s) 218, 383
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 66, 560, 569
NarI GGCGCC 1 cut(s) 135
NciI CCSGG 2 cut(s) 218, 383
NdeI CATATG 1 cut(s) 269
NdeII GATC 2 cut(s) 37, 574
NlaIII CATG 1 cut(s) 639
NlaIV GGNNCC 6 cut(s) 136, 222, 285, 366, 431, 515
PceI AGGCCT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfoI TCCNGGA 2 cut(s) 216, 381
PkrI GCNGC 3 cut(s) 171, 553, 556
PluTI GGCGCC 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 15
PspN4I GGNNCC 6 cut(s) 136, 222, 285, 366, 431, 515
PvuII CAGCTG 1 cut(s) 554
RseI CAYNNNNRTG 1 cut(s) 634
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 3 cut(s) 314, 330, 443
SatI GCNGC 3 cut(s) 170, 552, 555
Sau3AI GATC 2 cut(s) 37, 574
ScrFI CCNGG 2 cut(s) 218, 383
SduI GDGCHC 2 cut(s) 15, 225
SfaNI GCATC 1 cut(s) 475
SfoI GGCGCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 634
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 3 cut(s) 348, 444, 500
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 7 cut(s) 89, 167, 170, 225, 259, 544, 572
SspDI GGCGCC 1 cut(s) 134
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 216, 381
StyI CCWWGG 1 cut(s) 183
TaiI ACGT 1 cut(s) 524
TaqI TCGA 4 cut(s) 78, 189, 420, 469
TaqII GACCGA 1 cut(s) 62
TasI AATT 3 cut(s) 348, 444, 500
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 3 cut(s) 314, 330, 443
Tru9I TTAA 3 cut(s) 314, 330, 443
TseI GCWGC 2 cut(s) 551, 554
TspDTI ATGAA 5 cut(s) 14, 17, 151, 437, 624
TspGWI ACGGA 2 cut(s) 320, 386
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.