Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009212

Physical Location & Seq

Genomic Coordinates Reverse (-)

9738615 .. 9745545

Gene Structure 6931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1113600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3915 bp

ATGTTCTCTCCCGGAACCAAGCGACCCAATGCGAACCCTCGCCGGGATCTAGGATCTCCGGCCACGCCCCTCACCGAGAACCTCGGGCCCGGCTCGGACAACTCATTCCCGAATCGGCCCAGCACCGGCACCCCGGCTCCCTGGGCTCCTCGCTTATCTGTTCTCGCCAGGGTTTTACCGGCGAACCAAAATGAAAAGGGGGATGAAACTAAGCCCGTCTTTGTCGGAGAGTTTCCGCAAGTTGTTCGTGATGGACAGGCCAATATGCTGCAGAAGCATGCTCATGGTGATGCATATGTATCCGGTGGAATGGAGAGGGGAACGTCCCTGGCATGGATTATATGTGGAAACAGACTCTTTGTGTGGAGTTATCTGTCTCCTGCAGTGTCGACGAATTGCAATGTTCTTGAAATTCCTCTGAATGTCTTTGAAGGTGGGGATGTTGGCAGGAGTGGTGGGAACTGTTGGTTGCTTTGTGTTGTGAATGGGGATAGCGCGTCCACAAGAACAAAGAAAGTTGTAAAACATTGTAGCTCCGCTGGTATTGTATTGTGTCACAAGAAAACTCGAGCTGTTGTGTACTGGCCTGAAATTTATGCTGAAGAGAGAACTGACCCCATTGTTAGTGTTGCATCTGCTGATGAATTGGAGGTGAATTCTTCACCTATTGATAGGAAGACCACACCAAAACGGCAACAACTGATCAGCAGGCAGAGGGGTAGTTTGACTGGGTTTTGTACATTTAACTCTTTGATTGCTTCTGCGGTCCCTGATTCCCAGGATGTGTGCGTTGCACTTGCCTGTAGTTCTGATGGCCAGCTATGGCAGTTTCATTGCAGCCCCTCTGGTGTTAGCCGCAAGAAAGTATATCTGGACGATCAAACTCTTCCCTCCCTAGGGGGTGACAATGTTCAAATTTTAGGAAGTGAGGGCTATCCAAGGTCACTGACCTGGTGTTTCCCAAATCTTCAAATTCAGGACTCTAACCGGCAGTTTTTTCTTCTGACAGATCGTGAGATTCAATGCCTTAGTATTGAACTTGGTGCTGATTTCATTGTGTCAAAGCTCTGGTCTCATGAGATTATTGGCTCAGATGGTGATTCGGGAATAAAGAAAGATTTGGCCGGGCAGAAGCGAATCTGGCCGCTTGATATGCAGGTAGACTATCACGGTAAAGTGACTACCATTCTAGTTGCCACCTTCTGCCTGGACCGGGGTAGTAGTTCCAGCTACACACAGTACTCTCTTTTGACCATGCAATACAAATCTGGAATCAGTTTAGAGAAAAAAGCTCCTGTCCAAGTGATAATCCCAAAAGGAAGAGTAGAAGAGGAAGACTTCTTATTCTCCATGAAACTGCGAGTAGGAGGTAAGCCTCAAGGATCAGCAATCATACTTTCTGGTGATGGGACGGCAACAGTATCCCATTACTTTAGAAATTCTACACGGCTCTATAAATTTGACCTACCTTATGATGCTGGAAAGGTTCTAGATGCTTCAATTCTTCCATCTACAGATGATGGTGAAGAAGGGCCATGGGTTGTGCTTACTGAGAAGGCAGGGATATGGGCAATACCCGAGAAGGCTGTTATACTTGGTGGGGTTGAACCTCCTCAACGAAGTTTGTCACGTAAAGGTAGCTCAAATGAAGGATCTGCACAAGAAGAGAGAAAGAACCTCTCATTTTTGGGCAATATTGCGCCTAGAAGGGCTACTTCTGAAGCATGGGATGCTGGAGATAGACAAGGGGCTGTGACTGTAACTGCACGTCAAACTGCTCAAGATGAAGAATCAGAAACTTTACTTAGCCAGCTGTTCCAAGATTATCATTTATCTGGGCAAGTTGATGCCTCATTTGAAAAGCTAAAAAGATCTGGGGCATTCGAAAGGGATAGAGAGACGAATGTTTTTGCACGAATGAGCAAATCAATTGTTGACACTTTAGCTAAGCACTGGACAACAACTAGAGGAGCTGAGATTTTGGCAATGGCTGTTGTATCCTCCCAACTCATGGATAAGCAACAGAAGCATACAAAGTTTCTCCAGTTTCTTGCTTTATCCAAATGTCATGAGGAGCTGTGTTCAAGACAGAGACATTCTTTGCAAATTATCTTGGAACATGGTGAGAAGCTTGCTGGAATGATTCAACTAAGGGAATTGCAGAACATTATCAGCCATAAACGCTCCCGTGGACGTAGCCCATCCCGTTCTAGTCCAGAAAATCAAATATCAGGAGCTCTCTGGGATCTTATCCAGTTAGTTGGTGAGCGAGCCCGCCGGAATACTGTTCTTCTGATGGACAGGGACAATGCTGAAGTATTTTACAGTAAAGTTTCAGATCTTGAACAAGTTTTCGATTGCTTAGACAAACAGCTAGAGTATGTAATAAGTGCAGAGCAGCCATTTGAGATTCAGGTTCAGAGAGCTTGTGAACTGTCAAATGCATGTGTTACTATAGTTCGTACAGCCATGCAATACAGAAGTGAGCATCACTTATGGTATCCGCCACCTGAGAGCTTGACACCTTGGTATTCTCAAGCTGTGGTACGCAATGGGATGTGGCACCTCGCTTCCCTCATGCTGCAGCTGTTAAAGGAGGCATCTCAACTTGACGCGTCTGCCAAATTAGATTTATATACTCATCTGGAAGCGCTGGCTGAAGTTTTGCTGGAGGCATATGCTGGTGCTGTCACAGCTAAAGTTGAACAAGGAGGTGAACACAAAGGCTTATTGGATGAGTATTGGAACAGGAGGGATGCACTTCTGGACACCCTTTATCAAGAAGTCAAGGAATCTGTGGAAGCTGGGAATCAGAATTTAAATGAAGGAACTGAGGATCTAAAAGAAGAAATTCTTGCTAGGCTTTCGTCATGTTTGTTGCCCATGGCAAAACGGCATGAATGCTACAGTACATTATGGAAAATATGCTGTGATCTAAATGATTCTGGACTACTAAGAAATCTGATGCACGACAGCATGGGGCCTAATGGAGGGTTCAGTTACTATGTGTTCAAACAACTCTATCTGAGGAGGCAATATTCCAAACTTCTAAGGCTTGGGGAAGAGTTTCATGAAGAGCTATCCATATTCTTAAAGTATCATCAAGACCTTCTGTGGCTTCATGAAGTTTACCTTCATCAATTTTCCCCAGCTTCGGAAACTCTTCATGAATTAGCTCTTTCTCAAGCCGAAAACTCAATTTCCGAGGCTGAAGAGGGGACTGGTCCTACGTATCTGACTATGTTACCAAAACTGGCAGACAGAAAGCGTCTTTTGAATCTCTCAAAAATAGCTGCAATAGCAGGTAAAGATGCTAATTGTGAGACCAAAGTGAAGCGCATTGAGGCTGATTTGAAGATTCTAAAGTTGCAGGAAGAAATACTAAACCTACTTCCTGATGATGAAACAAAGCAAAGCCTTGACACAAAGCTACTTCATCCAGAAGATCTTATCAAGCTGTGCCTTGAGGGTGAAAGCGCAGAGCTCTCATTGCGGGCTTTTGATGTTTTTGCATGGACCAGCTCCTCTTTCCGTCAGACCCACACAAACCTTTTGGAGCATTGCTGGAGAAACGCTGCTGATCAAGATGATTGGAGTCAACTCTATCAAGCATCAGTATCCGAAGGGTGGGGTGATGAAGAAACCTTGCAGAACCTGAAAGACACCGTGCTCTTCCAGGCTTCAAACAGATGCTATGGACCCGAAGCTGAAACTTTTGGAGAAGGGTTTGAGGAAGTGTTGCCGCTGAGACAAGAAATTGCAGAGCCTACAGTCATGAAAGATACAGTTTCTTCTGTGGAAGCCGTACTCATGCAGCACAAAGATTACTCCGAGGCAGGCAAGCTAATGCTGACTGCAATCATGTTGGGTAGCTTACAGGATGATACCGAAGAAGGTCCTGTGCCTATGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000972 GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0006325 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0008104 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0017038 GO:0017056 GO:0019219 GO:0019222 GO:0031080 GO:0031081 GO:0031323 GO:0031326 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034629 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0051664 GO:0051668 GO:0060255 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nucleoporin_C Nucleoporin_N

Protein Analysis

1305

Amino Acids

145.6

Weight (kDa)

5.43

Isoelectric Point (pI)

47.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nucleoporin_N	PF08801	72 - 523	2.4e-32	Nup133 N terminal like
Nucleoporin_C	PF03177	998 - 1153	1.6e-06	Non-repetitive/WGA-negative nucleoporin C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009212)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05120 AT2G05120
fragaria_vesca	FvH4_6g40930 FvH4_6g40930 FvH4_6g40930 FvH4_6g40930 FvH4_6g40930
malus_domestica	MD17G1113600.v1.1
prunus_persica	Prupe.3G200800_v2.0.a1
pyrus_communis	pycom17g10520
rosa_chinensis	RchiOBHm_Chr2g0156551
rosa_laevigata	RLG00000020920
rosa_multiflora	Rmu_sc0002717.1_g000003
rosa_roxburghii	Rroxscaffold_2G00092950
rosa_rugosa	Rorug02G0454100 Rorug02G0454200 Rorug02G0454300
rosa_samantha	Rh2AG520000 Rh2BG532500 Rh2CG505000 Rh2DG541400
rosa_wichuraiana	Rw2G042870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1147, 3293
AccB1I	GGYRCC	2 cut(s)	128, 2562
AccB7I	CCANNNNNTGG	1 cut(s)	2011
AccI	GTMKAC	2 cut(s)	389, 1161
AccII	CGCG	2 cut(s)	497, 2615
AciI	CCGC	9 cut(s)	236, 537, 764, 856, 1145, 2275, 2504, 3493, 3743
AclWI	GGATC	6 cut(s)	54, 61, 1390, 1660, 2253, 2844
AcoI	YGGCCR	4 cut(s)	60, 814, 1122, 1142
AcsI	RAATTY	9 cut(s)	411, 591, 655, 915, 972, 1438, 1457, 2815, 2850
AcuI	CTGAAG	5 cut(s)	621, 1740, 2334, 2679, 3231
AfaI	GTAC	7 cut(s)	581, 739, 1241, 2464, 2547, 2911, 3807
AfeI	AGCGCT	1 cut(s)	2652
AflIII	ACRYGT	1 cut(s)	2613
AjiI	CACGTC	1 cut(s)	1769
AjnI	CCWGG	7 cut(s)	140, 167, 327, 777, 950, 1206, 3675
Alw21I	GWGCWC	3 cut(s)	2239, 3486, 3672
Alw26I	GTCTC	6 cut(s)	381, 1077, 1892, 2086, 3317, 3742
AlwI	GGATC	6 cut(s)	54, 61, 1390, 1660, 2253, 2844
AlwNI	CAGNNNCTG	1 cut(s)	3655
Ama87I	CYCGRG	3 cut(s)	83, 567, 1577
Aor51HI	AGCGCT	1 cut(s)	2652
ApaI	GGGCCC	1 cut(s)	90
ApeKI	GCWGC	8 cut(s)	268, 837, 2398, 2581, 2584, 3293, 3575, 3814
ApoI	RAATTY	9 cut(s)	411, 591, 655, 915, 972, 1438, 1457, 2815, 2850
ArsI	GACNNNNNNTTYG	2 cut(s)	1055, 1087
Asp700I	GAANNNNTTC	3 cut(s)	2850, 3066, 3162
AspA2I	CCTAGG	1 cut(s)	895
AspLEI	GCGC	5 cut(s)	497, 1702, 2653, 3339, 3479
AsuC2I	CCSGG	6 cut(s)	12, 44, 90, 134, 1126, 1214
AsuII	TTCGAA	1 cut(s)	1884
AvaI	CYCGRG	3 cut(s)	83, 567, 1577
AvaII	GGWCC	6 cut(s)	766, 1210, 3224, 3516, 3698, 3896
AvrII	CCTAGG	1 cut(s)	895
BaeGI	GKGCMC	1 cut(s)	90
BalI	TGGCCA	1 cut(s)	816
BanI	GGYRCC	2 cut(s)	128, 2562
BanII	GRGCYC	5 cut(s)	90, 148, 2239, 2275, 3486
BbsI	GAAGAC	2 cut(s)	683, 1341
Bbv12I	GWGCWC	3 cut(s)	2239, 3486, 3672
BbvI	GCAGC	8 cut(s)	255, 849, 2410, 2568, 2596, 3280, 3562, 3826
BccI	CCATC	8 cut(s)	245, 806, 1088, 1400, 1514, 1516, 2209, 2290
BceAI	ACGGC	5 cut(s)	707, 1428, 1463, 2909, 3788
BcgI	CGANNNNNNTGC	2 cut(s)	227, 261
BciT130I	CCWGG	7 cut(s)	142, 169, 329, 779, 952, 1208, 3677
BciVI	GTATCC	5 cut(s)	310, 1432, 2008, 2511, 3628
BclI	TGATCA	2 cut(s)	702, 3580
BcnI	CCSGG	6 cut(s)	12, 44, 90, 134, 1126, 1214
BcoDI	GTCTC	6 cut(s)	381, 1077, 1892, 2086, 3317, 3742
BfaI	CTAG	9 cut(s)	50, 896, 1190, 1490, 1704, 1965, 2211, 2375, 2859
BfmI	CTRYAG	8 cut(s)	269, 381, 802, 1512, 2454, 2582, 2905, 3768
BfoI	RGCGCY	1 cut(s)	2654
BfuAI	ACCTGC	2 cut(s)	1147, 3293
BfuI	GTATCC	5 cut(s)	310, 1432, 2008, 2511, 3628
BglII	AGATCT	3 cut(s)	1871, 2338, 3445
BlnI	CCTAGG	1 cut(s)	895
BlpI	GCTNAGC	1 cut(s)	1947
BmcAI	AGTACT	1 cut(s)	1241
Bme18I	GGWCC	6 cut(s)	766, 1210, 3224, 3516, 3698, 3896
BmeT110I	CYCGRG	3 cut(s)	83, 567, 1577
BmgBI	CACGTC	1 cut(s)	1769
BmiI	GGNNCC	9 cut(s)	16, 88, 130, 138, 147, 768, 2564, 2982, 3700
BmrI	ACTGGG	1 cut(s)	738
BmuI	ACTGGG	1 cut(s)	738
BpiI	GAAGAC	2 cut(s)	683, 1341
BpmI	CTGGAG	4 cut(s)	1755, 2027, 2690, 3586
Bpu1102I	GCTNAGC	1 cut(s)	1947
Bpu14I	TTCGAA	1 cut(s)	1884
BpuEI	CTTGAG	5 cut(s)	1362, 1764, 2520, 3168, 3485
BpuMI	CCSGG	6 cut(s)	12, 44, 90, 134, 1126, 1214
BsaAI	YACGTR	2 cut(s)	1631, 3231
BsaI	GGTCTC	2 cut(s)	1077, 3317
BsaWI	WCCGGW	1 cut(s)	302
BsaXI	ACNNNNNCTCC	2 cut(s)	121, 151
Bse118I	RCCGGY	3 cut(s)	125, 178, 987
Bse1I	ACTGG	7 cut(s)	587, 733, 1958, 2044, 2254, 3226, 3258
Bse3DI	GCAATG	6 cut(s)	406, 832, 1992, 2557, 3488, 3559
BseBI	CCWGG	7 cut(s)	142, 169, 329, 779, 952, 1208, 3677
BseMI	GCAATG	6 cut(s)	406, 832, 1992, 2557, 3488, 3559
BseMII	CTCAG	7 cut(s)	1104, 1542, 1965, 2502, 2823, 3017, 3737
BseNI	ACTGG	7 cut(s)	587, 733, 1958, 2044, 2254, 3226, 3258
BseRI	GAGGAG	6 cut(s)	138, 1602, 1983, 2087, 3043, 3514
BseSI	GKGCMC	1 cut(s)	90
BseXI	GCAGC	8 cut(s)	255, 849, 2410, 2568, 2596, 3280, 3562, 3826
BseYI	CCCAGC	3 cut(s)	119, 2804, 3148
BsgI	GTGCAG	3 cut(s)	1641, 1749, 2412
Bsh1236I	CGCG	2 cut(s)	497, 2615
BshNI	GGYRCC	2 cut(s)	128, 2562
BsiHKAI	GWGCWC	3 cut(s)	2239, 3486, 3672
BsiHKCI	CYCGRG	3 cut(s)	83, 567, 1577
BslFI	GGGAC	5 cut(s)	310, 752, 1423, 2318, 3232
BsmAI	GTCTC	6 cut(s)	381, 1077, 1892, 2086, 3317, 3742
BsmBI	CGTCTC	1 cut(s)	1892
BsmFI	GGGAC	5 cut(s)	310, 752, 1423, 2318, 3232
BsmI	GAATGC	2 cut(s)	1880, 2906
Bso31I	GGTCTC	2 cut(s)	1077, 3317
BsoBI	CYCGRG	3 cut(s)	83, 567, 1577
Bsp119I	TTCGAA	1 cut(s)	1884
Bsp120I	GGGCCC	1 cut(s)	86
Bsp1286I	GDGCHC	6 cut(s)	90, 148, 2239, 2275, 3486, 3672
Bsp1407I	TGTACA	1 cut(s)	737
Bsp1720I	GCTNAGC	1 cut(s)	1947
Bsp19I	CCATGG	2 cut(s)	1535, 2883
BspACI	CCGC	9 cut(s)	236, 537, 764, 856, 1145, 2275, 2504, 3493, 3743
BspCNI	CTCAG	7 cut(s)	1103, 1543, 1966, 2503, 2824, 3018, 3738
BspFNI	CGCG	2 cut(s)	497, 2615
BspHI	TCATGA	6 cut(s)	1075, 2068, 3070, 3121, 3166, 3774
BspLI	GGNNCC	9 cut(s)	16, 88, 130, 138, 147, 768, 2564, 2982, 3700
BspMAI	CTGCAG	3 cut(s)	273, 385, 2586
BspMI	ACCTGC	2 cut(s)	1147, 3293
BspPI	GGATC	6 cut(s)	54, 61, 1390, 1660, 2253, 2844
BspQI	GCTCTTC	2 cut(s)	3069, 3677
BspT104I	TTCGAA	1 cut(s)	1884
BspT107I	GGYRCC	2 cut(s)	128, 2562
BspTNI	GGTCTC	2 cut(s)	1077, 3317
BsrDI	GCAATG	6 cut(s)	406, 832, 1992, 2557, 3488, 3559
BsrFI	RCCGGY	3 cut(s)	125, 178, 987
BsrGI	TGTACA	1 cut(s)	737
BsrI	ACTGG	7 cut(s)	587, 733, 1958, 2044, 2254, 3226, 3258
BssAI	RCCGGY	3 cut(s)	125, 178, 987
BssT1I	CCWWGG	5 cut(s)	895, 938, 1535, 2525, 2883
Bst2UI	CCWGG	7 cut(s)	142, 169, 329, 779, 952, 1208, 3677
BstAPI	GCANNNNNTGC	1 cut(s)	1730
BstAUI	TGTACA	1 cut(s)	737
BstBAI	YACGTR	2 cut(s)	1631, 3231
BstBI	TTCGAA	1 cut(s)	1884
BstDSI	CCRYGG	3 cut(s)	1535, 2188, 2883
BstENI	CCTNNNNNAGG	1 cut(s)	2988
BstFNI	CGCG	2 cut(s)	497, 2615
BstH2I	RGCGCY	1 cut(s)	2654
BstHHI	GCGC	5 cut(s)	497, 1702, 2653, 3339, 3479
BstMAI	GTCTC	6 cut(s)	381, 1077, 1892, 2086, 3317, 3742
BstNI	CCWGG	7 cut(s)	142, 169, 329, 779, 952, 1208, 3677
BstNSI	RCATGY	2 cut(s)	281, 2448
BstSFI	CTRYAG	8 cut(s)	269, 381, 802, 1512, 2454, 2582, 2905, 3768
BstSLI	GKGCMC	1 cut(s)	90
BstSNI	TACGTA	1 cut(s)	3231
BstUI	CGCG	2 cut(s)	497, 2615
BstV1I	GCAGC	8 cut(s)	255, 849, 2410, 2568, 2596, 3280, 3562, 3826
BstV2I	GAAGAC	2 cut(s)	683, 1341
BstX2I	RGATCY	8 cut(s)	46, 53, 1652, 1871, 2245, 2338, 2836, 3445
BstXI	CCANNNNNNTGG	1 cut(s)	2261
BstYI	RGATCY	8 cut(s)	46, 53, 1652, 1871, 2245, 2338, 2836, 3445
BsuI	GTATCC	5 cut(s)	310, 1432, 2008, 2511, 3628
BtgI	CCRYGG	3 cut(s)	1535, 2188, 2883
BtrI	CACGTC	1 cut(s)	1769
BtsI	GCAGTG	1 cut(s)	390
BtsIMutI	CAGTG	3 cut(s)	390, 944, 1951
BveI	ACCTGC	2 cut(s)	1147, 3293
CaiI	CAGNNNCTG	1 cut(s)	3655
CciI	TCATGA	6 cut(s)	1075, 2068, 3070, 3121, 3166, 3774
CfoI	GCGC	5 cut(s)	497, 1702, 2653, 3339, 3479
Cfr10I	RCCGGY	3 cut(s)	125, 178, 987
CseI	GACGC	4 cut(s)	486, 2604, 2621, 3257
CsiI	ACCWGGT	1 cut(s)	950
Csp6I	GTAC	7 cut(s)	580, 738, 1240, 2463, 2546, 2910, 3806
CviQI	GTAC	7 cut(s)	580, 738, 1240, 2463, 2546, 2910, 3806
DraI	TTTAAA	1 cut(s)	2820
EaeI	YGGCCR	4 cut(s)	60, 814, 1122, 1142
EciI	GGCGGA	1 cut(s)	2493
Ecl136II	GAGCTC	2 cut(s)	2237, 3484
Eco105I	TACGTA	1 cut(s)	3231
Eco130I	CCWWGG	5 cut(s)	895, 938, 1535, 2525, 2883
Eco24I	GRGCYC	5 cut(s)	90, 148, 2239, 2275, 3486
Eco31I	GGTCTC	2 cut(s)	1077, 3317
Eco47I	GGWCC	6 cut(s)	766, 1210, 3224, 3516, 3698, 3896
Eco47III	AGCGCT	1 cut(s)	2652
Eco53kI	GAGCTC	2 cut(s)	2237, 3484
Eco57I	CTGAAG	5 cut(s)	621, 1740, 2334, 2679, 3231
Eco88I	CYCGRG	3 cut(s)	83, 567, 1577
EcoICRI	GAGCTC	2 cut(s)	2237, 3484
EcoNI	CCTNNNNNAGG	1 cut(s)	2988
EcoO109I	RGGNCCY	2 cut(s)	2981, 3896
EcoRI	GAATTC	1 cut(s)	655
EcoRII	CCWGG	7 cut(s)	140, 167, 327, 777, 950, 1206, 3675
EcoT14I	CCWWGG	5 cut(s)	895, 938, 1535, 2525, 2883
EcoT22I	ATGCAT	2 cut(s)	295, 2446
EcoT38I	GRGCYC	5 cut(s)	90, 148, 2239, 2275, 3486
ErhI	CCWWGG	5 cut(s)	895, 938, 1535, 2525, 2883
Esp3I	CGTCTC	1 cut(s)	1892
FalI	AAGNNNNNCTT	8 cut(s)	203, 235, 1699, 1731, 2838, 2870, 3117, 3149
FaqI	GGGAC	5 cut(s)	310, 752, 1423, 2318, 3232
FauI	CCCGC	2 cut(s)	2282, 3486
FauNDI	CATATG	2 cut(s)	295, 2677
FbaI	TGATCA	2 cut(s)	702, 3580
FblI	GTMKAC	2 cut(s)	389, 1161
FriOI	GRGCYC	5 cut(s)	90, 148, 2239, 2275, 3486
FspBI	CTAG	9 cut(s)	50, 896, 1190, 1490, 1704, 1965, 2211, 2375, 2859
GlaI	GCGC	5 cut(s)	496, 1701, 2652, 3338, 3478
GsaI	CCCAGC	3 cut(s)	123, 2808, 3152
GsuI	CTGGAG	4 cut(s)	1755, 2027, 2690, 3586
HaeII	RGCGCY	1 cut(s)	2654
HgaI	GACGC	4 cut(s)	486, 2604, 2621, 3257
HhaI	GCGC	5 cut(s)	497, 1702, 2653, 3339, 3479
Hin6I	GCGC	5 cut(s)	495, 1700, 2651, 3337, 3477
HinP1I	GCGC	5 cut(s)	495, 1700, 2651, 3337, 3477
HincII	GTYRAC	3 cut(s)	390, 1936, 3599
HindII	GTYRAC	3 cut(s)	390, 1936, 3599
HindIII	AAGCTT	1 cut(s)	2129
Hpy99I	CGWCG	1 cut(s)	394
HpyCH4IV	ACGT	5 cut(s)	323, 1630, 1768, 2194, 3230
HpySE526I	ACGT	5 cut(s)	323, 1630, 1768, 2194, 3230
HspAI	GCGC	5 cut(s)	495, 1700, 2651, 3337, 3477
Ksp22I	TGATCA	2 cut(s)	702, 3580
LguI	GCTCTTC	2 cut(s)	3069, 3677
Lsp1109I	GCAGC	8 cut(s)	255, 849, 2410, 2568, 2596, 3280, 3562, 3826
MabI	ACCWGGT	1 cut(s)	950
MaeI	CTAG	9 cut(s)	50, 896, 1190, 1490, 1704, 1965, 2211, 2375, 2859
MaeII	ACGT	5 cut(s)	323, 1630, 1768, 2194, 3230
MfeI	CAATTG	1 cut(s)	1929
MflI	RGATCY	8 cut(s)	46, 53, 1652, 1871, 2245, 2338, 2836, 3445
MhlI	GDGCHC	6 cut(s)	90, 148, 2239, 2275, 3486, 3672
MlsI	TGGCCA	1 cut(s)	816
MluI	ACGCGT	1 cut(s)	2613
MluNI	TGGCCA	1 cut(s)	816
MlyI	GAGTC	3 cut(s)	348, 974, 3604
MmeI	TCCRAC	1 cut(s)	205
Mox20I	TGGCCA	1 cut(s)	816
Mph1103I	ATGCAT	2 cut(s)	295, 2446
MroXI	GAANNNNTTC	3 cut(s)	2850, 3066, 3162
MscI	TGGCCA	1 cut(s)	816
MseI	TTAA	4 cut(s)	744, 2591, 2819, 3092
MslI	CAYNNNNRTG	2 cut(s)	282, 288
Msp20I	TGGCCA	1 cut(s)	816
MspA1I	CMGCKG	4 cut(s)	539, 1813, 2587, 3745
MunI	CAATTG	1 cut(s)	1929
Mva1269I	GAATGC	2 cut(s)	1880, 2906
MvaI	CCWGG	7 cut(s)	142, 169, 329, 779, 952, 1208, 3677
MvnI	CGCG	2 cut(s)	497, 2615
NciI	CCSGG	6 cut(s)	12, 44, 90, 134, 1126, 1214
NcoI	CCATGG	2 cut(s)	1535, 2883
NdeI	CATATG	2 cut(s)	295, 2677
NlaIV	GGNNCC	9 cut(s)	16, 88, 130, 138, 147, 768, 2564, 2982, 3700
NmuCI	GTSAC	7 cut(s)	554, 902, 942, 1177, 1626, 1753, 2689
NsiI	ATGCAT	2 cut(s)	295, 2446
NspI	RCATGY	2 cut(s)	281, 2448
NspV	TTCGAA	1 cut(s)	1884
PaeI	GCATGC	1 cut(s)	281
PaeR7I	CTCGAG	1 cut(s)	567
PagI	TCATGA	6 cut(s)	1075, 2068, 3070, 3121, 3166, 3774
PasI	CCCWGGG	1 cut(s)	141
PciSI	GCTCTTC	2 cut(s)	3069, 3677
PctI	GAATGC	2 cut(s)	1880, 2906
PdmI	GAANNNNTTC	3 cut(s)	2850, 3066, 3162
PflMI	CCANNNNNTGG	1 cut(s)	2011
PfoI	TCCNGGA	1 cut(s)	10
PleI	GAGTC	3 cut(s)	348, 974, 3603
PpsI	GAGTC	3 cut(s)	348, 974, 3603
Ppu21I	YACGTR	2 cut(s)	1631, 3231
PpuMI	RGGWCCY	1 cut(s)	3896
Psp124BI	GAGCTC	2 cut(s)	2239, 3486
Psp5II	RGGWCCY	1 cut(s)	3896
Psp6I	CCWGG	7 cut(s)	140, 167, 327, 777, 950, 1206, 3675
PspFI	CCCAGC	3 cut(s)	119, 2804, 3148
PspGI	CCWGG	7 cut(s)	140, 167, 327, 777, 950, 1206, 3675
PspN4I	GGNNCC	9 cut(s)	16, 88, 130, 138, 147, 768, 2564, 2982, 3700
PspOMI	GGGCCC	1 cut(s)	86
PspPPI	RGGWCCY	1 cut(s)	3896
PspXI	VCTCGAGB	1 cut(s)	567
PstI	CTGCAG	3 cut(s)	273, 385, 2586
PstNI	CAGNNNCTG	1 cut(s)	3655
PsuI	RGATCY	8 cut(s)	46, 53, 1652, 1871, 2245, 2338, 2836, 3445
PvuII	CAGCTG	2 cut(s)	1813, 2587
RsaI	GTAC	7 cut(s)	581, 739, 1241, 2464, 2547, 2911, 3807
RsaNI	GTAC	7 cut(s)	580, 738, 1240, 2463, 2546, 2910, 3806
RseI	CAYNNNNRTG	2 cut(s)	282, 288
SacI	GAGCTC	2 cut(s)	2239, 3486
SalI	GTCGAC	1 cut(s)	388
SapI	GCTCTTC	2 cut(s)	3069, 3677
SaqAI	TTAA	4 cut(s)	744, 2591, 2819, 3092
ScaI	AGTACT	1 cut(s)	1241
SchI	GAGTC	3 cut(s)	348, 974, 3604
SduI	GDGCHC	6 cut(s)	90, 148, 2239, 2275, 3486, 3672
SexAI	ACCWGGT	1 cut(s)	950
SfcI	CTRYAG	8 cut(s)	269, 381, 802, 1512, 2454, 2582, 2905, 3768
Sfr274I	CTCGAG	1 cut(s)	567
SfuI	TTCGAA	1 cut(s)	1884
SinI	GGWCC	6 cut(s)	766, 1210, 3224, 3516, 3698, 3896
SlaI	CTCGAG	1 cut(s)	567
SmiI	ATTTAAAT	1 cut(s)	2820
SmiMI	CAYNNNNRTG	2 cut(s)	282, 288
SmlI	CTYRAG	6 cut(s)	567, 1377, 1779, 2535, 3183, 3464
SmoI	CTYRAG	6 cut(s)	567, 1377, 1779, 2535, 3183, 3464
SnaBI	TACGTA	1 cut(s)	3231
SphI	GCATGC	1 cut(s)	281
SsiI	CCGC	9 cut(s)	236, 537, 764, 856, 1145, 2275, 2504, 3493, 3743
SspI	AATATT	2 cut(s)	1696, 3038
SspMI	CTAG	9 cut(s)	50, 896, 1190, 1490, 1704, 1965, 2211, 2375, 2859
SstI	GAGCTC	2 cut(s)	2239, 3486
StyI	CCWWGG	5 cut(s)	895, 938, 1535, 2525, 2883
SwaI	ATTTAAAT	1 cut(s)	2820
TaiI	ACGT	5 cut(s)	326, 1633, 1771, 2197, 3233
TaqI	TCGA	4 cut(s)	389, 568, 1884, 2355
TatI	WGTACW	4 cut(s)	579, 737, 1239, 2909
TauI	GCSGC	3 cut(s)	858, 1147, 3745
Tru1I	TTAA	4 cut(s)	744, 2591, 2819, 3092
Tru9I	TTAA	4 cut(s)	744, 2591, 2819, 3092
TscAI	CASTG	3 cut(s)	390, 951, 1958
TseFI	GTSAC	7 cut(s)	554, 902, 942, 1177, 1626, 1753, 2689
TseI	GCWGC	8 cut(s)	268, 837, 2398, 2581, 2584, 3293, 3575, 3814
Tsp45I	GTSAC	7 cut(s)	554, 902, 942, 1177, 1626, 1753, 2689
TspGWI	ACGGA	1 cut(s)	3521
TspRI	CASTG	3 cut(s)	390, 951, 1958
Van91I	CCANNNNNTGG	1 cut(s)	2011
VpaK11BI	GGWCC	6 cut(s)	766, 1210, 3224, 3516, 3698, 3896
XagI	CCTNNNNNAGG	1 cut(s)	2988
XapI	RAATTY	9 cut(s)	411, 591, 655, 915, 972, 1438, 1457, 2815, 2850
XbaI	TCTAGA	1 cut(s)	1489
XceI	RCATGY	2 cut(s)	281, 2448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1204
XhoI	CTCGAG	1 cut(s)	567
XmaJI	CCTAGG	1 cut(s)	895
XmiI	GTMKAC	2 cut(s)	389, 1161
XmnI	GAANNNNTTC	3 cut(s)	2850, 3066, 3162
XspI	CTAG	9 cut(s)	50, 896, 1190, 1490, 1704, 1965, 2211, 2375, 2859
ZrmI	AGTACT	1 cut(s)	1241
Zsp2I	ATGCAT	2 cut(s)	295, 2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.