Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0009212

Physical Location & Seq

Genomic Coordinates Forward (+)

77091971 .. 77098945

Gene Structure 6975 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG541400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3084 bp

ATGGAGAGAGGGACATTGGCGTGGATTATATGTGGAGGCAGACTCTTTGTGTGGAGTTATTTGTCTCCTGGTTGTTCGACGAGTTGCACTGTTCTTGAGATCCCTGGAAGCGTTTTCGAAGGTGGGGAGGCTGGGAGGACCGGTGGGAACTCGTGGTTGCTCTGCGTTGTTAATTGGGATAACACATCTACAAGAACAAAGAAAGTTGTGAAACAATGTAGCTCTGTTGGTATTGTACTGTGTAACAAAAAGACCCGCGCTGCTGTGTATTGGCCTGACATATACGGTGAAGGGAGAACTGATCCGGTTGTTAGTGTTGCATCTTCTGATGAATTGGAGGTGGCGTCCTCACCCATTGATAGGAAGACTCCCCAAAGGAGGCAGCAGTTACTTAGCAGGCAAGGGAGTAGTTTGAGTGGATCTTGTACTTTTAACTCTCTGATTGCGTGTGCAGTCCCTGATTCCCGTAATGAGTGTGTTGCAGTTGCCTGTAGTTCAAATGGTGAGCTATGGCAGTTTCATTGTTGTCCATCTGGTGTTAGCCGTAAGAAAGTGTATCAACACAATCAGAACTCATCCCCCCAAGGGGGTTACAGTGGCAGCAAAGGCTATCCAAGGTCATTGACCTGGTGCTTTCCAAATCTTCATGTGCAGGAATCCAGCCGGCAATTTGTTGTTCTGACAGATCGTGAGATGCAATGTTTTAGCATTGAACTTTCTCCTGATTATGTTGTGTCAAAGCTCTGGTCTCATGAGATTATTGGCTCGGATGGTGATCGGGGTATCAAGAAGGATTTGGCCGGGCAGAAGCGAATCTGGCCTCTTGACATGCAGGTAGACTATCATGGTAAAGTGACTACCATTCTAGTTGCTACCTTCTGCTTGGACCGGGGTAGTAGTTCAAGCTACGTGCAGTATTCTCTTTTGACCATGCAGTTTAAATCTGGAGGTAGTATAGCACCTACTCAAGAAACAGTTTTAGAGAGAAAAGATCCTATCCAGGTGATCATTCCAAAAGCAAGAGTAGAAGATGAAGACTTCTTATTCTCCATGAGACTGCGGGTAGGGGGAAAGCCTTCCGGATCAGCAATTATACTTTCTGGTGATGGAACCGCTACAGTATCCCATTATTATAGAACGACTACACGGCTTTATAAATTTGACCTACCTTATGATGCTGGAAAGGTTCTAGATGCTTCAATTCTTCCCTCTACAGATGATGGTGAAGAAGGGGCATGGGTTGTTCTAACTGAGAAGGCAGGAATATGGGCAATACCCGAGAAGGCTGTTATGATTGGTGGAGTTGAACCTCCTGAGCGAAGTTTATCACGTAAAGGTAGCTCAAATGAAGGATCCGCACAAGAAGAGAGAAAGAACCTTACATTTGCAGGGAATCTTGCTCCTAGAAGGGCTAGTTCTGAAGCATGGGATGCTGGAGATAGACAAAGGGTTATGCCTGTAATTGCACGTCAAACTGCCCAAGATGAAGAATCAGAAACTTTACTGAGCCAGCTTTTCCATGATTATCATTTACATGGGCAGGTTGATGCATCACTTGAAAAGCTAAAAATTGCAGGGGCATTTGAAAGGGATAGAGAGACAAATGTTTTTGCACGACTGAGCAAATCAATTGTTGATACCTTAGCTAAACATTGGACAACAACCAGAGGAGCTGAGATTTTGGCAATGGCCGTGGTATCCTCCCAACTCACGGATAAGCAACAGAAGCATACAAAATTTCTGGAGTTTCTTGCTCTATCCAAATGTCATGAGGAGCTATGTTCTAGACAGAGACATTCTTTGCAAATTATCTTGGAACACGGTGAAAAGCTTGCTGGAATGATTCAACTACGGGAATTGCAGAACATCATCAGCCAGAACCGCTCCAGTGGACTCAGCACCTCCCGTTCTAGTCCAGAAAATCAAATATCTGGTGCTCTGTGGGATCTTATTCAATTAGTTGGTGATAGAGCCCGTCGGAATACGGTGCTTCTAATGGACAGGGACAATGCTGAAGTATTCTACAGTAAAGTTTCTGATCTTCAAGAAGTGTTTTCTTGCTTAGACAAGCAGCTGGAGTATGTAATCCCTGCAGAGCAGCCATATGGGATTCAGGTCCAGAGAGCTTGTGAACTCTCAAATGCATGTGTTAGTATTGTCCGTGCAGCCATGCAATACAGAAACACACATCACTTGTGGTATCCCCCACCTGAGTGCTTAACACCCTGGTATTGCCAAGCTGTTGTACGCAATGGGATGTGGCAGGTTGCTTCCTTCATGCTGCAACTGTTAAAAGAAGCATCTCAGATTGATATGTCTGCAAAATCAGATTTATATTCTCATCTAGAACTGCTGGCTGAAGTTCTGCTTGAGGCATATACTGGTTCTGTCACCGCTAAAGTTGAACTTGGTCACGAACACAAGGGCTTATTAGATGAGTATTGGAATAGAAGGGATGCACTCCTAGACTCACTTTATCACCAAGTCAAAGATTTTGTGGAAGTTGGGCATGAGAATTTGAATGAAGGATCTGATGAGCTCAATGAAGAAATTTTTGGGAAGCTTTCTTCATGTTTGCTTCCCATGGCAAAGCGGCATGAATGCTACACTACTTTATGGAAAATATGCTGTGACATTAATGATTCTGAACTACTAAAAAATCTGATGCATGACAGCATGGGACCTAATGGAGGGTTCAGTTACTATGTGTTCAAACAACTATATGCGAGGAAACAATTTTCCAAACTTCTCAGGCTTGGGGAAGAGTTTCACGAAGCGCTCTCAATATTTTTAAAGTATCATCCAGATCTTCTGTGGCTTCATGAAGTGTTTCTTCATCAGTTTTCTTCAGCTTCAGACACCCTTCATGAATTAGCTCTTTCTCAAGAAGAAAGCTCAATTTCAGAGCCTGAAGATGGGACTGGTCCTGGGCATCTCACTACATTACCAAAGCTGGCAGATAGAAAGCGTTTTTTGAATCTCTCAAAAATAGCTGCAATTGCAGGTCCTTGTTTTTTTTTTTTAATCTTCCTTATACTGCATAACTTTTATTTGAACAGAGTTTTATTTTTATTGCCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000972 GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005737 GO:0006325 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0008104 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0017038 GO:0017056 GO:0019219 GO:0019222 GO:0031080 GO:0031081 GO:0031323 GO:0031326 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034629 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051641 GO:0051649 GO:0051664 GO:0051668 GO:0060255 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nucleoporin_C Nucleoporin_N

Protein Analysis

1027

Amino Acids

115.08

Weight (kDa)

6.14

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nucleoporin_N	PF08801	7 - 423	5.9e-31	Nup133 N terminal like
Nucleoporin_C	PF03177	899 - 965	8e-07	Non-repetitive/WGA-negative nucleoporin C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009212)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05120 AT2G05120
fragaria_vesca	FvH4_6g40930 FvH4_6g40930 FvH4_6g40930 FvH4_6g40930 FvH4_6g40930
malus_domestica	MD17G1113600.v1.1
prunus_persica	Prupe.3G200800_v2.0.a1
pyrus_communis	pycom17g10520
rosa_chinensis	RchiOBHm_Chr2g0156551
rosa_laevigata	RLG00000020920
rosa_multiflora	Rmu_sc0002717.1_g000003
rosa_roxburghii	Rroxscaffold_2G00092950
rosa_rugosa	Rorug02G0454100 Rorug02G0454200 Rorug02G0454300
rosa_samantha	Rh2AG520000 Rh2BG532500 Rh2CG505000 Rh2DG541400
rosa_wichuraiana	Rw2G042870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1155
Acc36I	ACCTGC	4 cut(s)	823, 1531, 2254, 2993
AccBSI	CCGCTC	1 cut(s)	1884
AccI	GTMKAC	1 cut(s)	837
AccII	CGCG	1 cut(s)	258
AccIII	TCCGGA	1 cut(s)	1079
AciI	CCGC	7 cut(s)	256, 1060, 1113, 1356, 1882, 2394, 2593
AclWI	GGATC	9 cut(s)	94, 296, 427, 986, 1090, 1347, 1360, 1953, 2536
AcoI	YGGCCR	2 cut(s)	798, 1689
AcsI	RAATTY	4 cut(s)	1157, 1736, 2515, 2550
AcuI	CTGAAG	6 cut(s)	1440, 2034, 2379, 2832, 2838, 2931
AcyI	GRCGYC	1 cut(s)	344
AfaI	GTAC	3 cut(s)	237, 427, 2247
AfeI	AGCGCT	1 cut(s)	2778
AfiI	CCNNNNNNNGG	8 cut(s)	360, 378, 585, 586, 587, 1711, 2690, 2915
AgeI	ACCGGT	1 cut(s)	140
AjiI	CACGTC	1 cut(s)	1469
AjnI	CCWGG	6 cut(s)	67, 103, 626, 999, 2225, 2926
AjuI	GAANNNNNNNTTGG	2 cut(s)	2538, 2570
AleI	CACNNNNGTG	1 cut(s)	2212
Alw21I	GWGCWC	2 cut(s)	1939, 2541
Alw26I	GTCTC	5 cut(s)	69, 753, 1048, 1592, 1786
AlwI	GGATC	9 cut(s)	94, 296, 427, 986, 1090, 1347, 1360, 1953, 2536
AlwNI	CAGNNNCTG	2 cut(s)	458, 2909
Ama87I	CYCGRG	1 cut(s)	1277
Aor13HI	TCCGGA	1 cut(s)	1079
Aor51HI	AGCGCT	1 cut(s)	2778
AoxI	GGCC	4 cut(s)	272, 798, 818, 1689
ApeKI	GCWGC	8 cut(s)	260, 382, 600, 2071, 2098, 2165, 2281, 2993
ApoI	RAATTY	4 cut(s)	1157, 1736, 2515, 2550
ArsI	GACNNNNNNTTYG	2 cut(s)	731, 763
AseI	ATTAAT	1 cut(s)	2637
AsiGI	ACCGGT	1 cut(s)	140
Asp700I	GAANNNNTTC	3 cut(s)	1075, 2766, 2829
AspLEI	GCGC	2 cut(s)	260, 2779
AspS9I	GGNCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
AsuC2I	CCSGG	2 cut(s)	802, 890
AsuII	TTCGAA	1 cut(s)	117
AvaI	CYCGRG	1 cut(s)	1277
AvaII	GGWCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
BamHI	GGATCC	1 cut(s)	1352
BanII	GRGCYC	2 cut(s)	1975, 2541
BauI	CACGAG	1 cut(s)	151
BbsI	GAAGAC	2 cut(s)	371, 1041
Bbv12I	GWGCWC	2 cut(s)	1939, 2541
BbvI	GCAGC	8 cut(s)	247, 394, 612, 2083, 2110, 2177, 2268, 2980
BccI	CCATC	5 cut(s)	538, 764, 1100, 1214, 2909
BceAI	ACGGC	3 cut(s)	528, 1163, 1676
BciT130I	CCWGG	6 cut(s)	69, 105, 628, 1001, 2227, 2928
BciVI	GTATCC	3 cut(s)	1132, 1708, 2211
BclI	TGATCA	1 cut(s)	1005
BcnI	CCSGG	2 cut(s)	802, 890
BcoDI	GTCTC	5 cut(s)	69, 753, 1048, 1592, 1786
BfaI	CTAG	8 cut(s)	866, 1190, 1404, 1413, 1785, 1911, 2345, 2465
BfmI	CTRYAG	5 cut(s)	490, 1116, 1212, 2023, 2091
BfoI	RGCGCY	1 cut(s)	2780
BfuAI	ACCTGC	4 cut(s)	823, 1531, 2254, 2993
BfuI	GTATCC	3 cut(s)	1132, 1708, 2211
BglII	AGATCT	1 cut(s)	2806
BisI	GCNGC	9 cut(s)	261, 383, 601, 2072, 2099, 2166, 2282, 2594, 2994
BlsI	GCNGC	9 cut(s)	262, 384, 602, 2073, 2100, 2167, 2283, 2595, 2995
Bme1390I	CCNGG	8 cut(s)	69, 105, 628, 802, 890, 1001, 2227, 2928
Bme18I	GGWCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
BmeT110I	CYCGRG	1 cut(s)	1277
BmgBI	CACGTC	1 cut(s)	1469
BmgT120I	GGNCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
BmiI	GGNNCC	3 cut(s)	1111, 1354, 2682
BmrFI	CCNGG	8 cut(s)	69, 105, 628, 802, 890, 1001, 2227, 2928
BpiI	GAAGAC	2 cut(s)	371, 1041
BplI	GAGNNNNNCTC	2 cut(s)	27, 59
BpmI	CTGGAG	5 cut(s)	966, 1455, 1763, 1870, 2096
Bpu10I	CCTNAGC	2 cut(s)	1314, 1642
Bpu14I	TTCGAA	1 cut(s)	117
BpuEI	CTTGAG	4 cut(s)	116, 951, 2390, 2868
BpuMI	CCSGG	2 cut(s)	802, 890
BsaAI	YACGTR	2 cut(s)	910, 1331
BsaBI	GATNNNNATC	1 cut(s)	774
BsaHI	GRCGYC	1 cut(s)	344
BsaI	GGTCTC	1 cut(s)	753
BsaJI	CCNNGG	8 cut(s)	103, 583, 614, 889, 1692, 2225, 2583, 2927
BsaWI	WCCGGW	3 cut(s)	140, 304, 1079
BsaXI	ACNNNNNCTCC	4 cut(s)	127, 157, 329, 359
Bsc4I	CCNNNNNNNGG	8 cut(s)	360, 378, 585, 586, 587, 1711, 2690, 2915
Bse118I	RCCGGY	2 cut(s)	140, 663
Bse1I	ACTGG	3 cut(s)	1887, 2386, 2926
Bse3DI	GCAATG	3 cut(s)	704, 1692, 2257
Bse8I	GATNNNNATC	1 cut(s)	774
BseAI	TCCGGA	1 cut(s)	1079
BseBI	CCWGG	6 cut(s)	69, 105, 628, 1001, 2227, 2928
BseDI	CCNNGG	8 cut(s)	103, 583, 614, 889, 1692, 2225, 2583, 2927
BseGI	GGATG	6 cut(s)	575, 775, 1435, 2262, 2461, 2800
BseJI	GATNNNNATC	1 cut(s)	774
BseLI	CCNNNNNNNGG	8 cut(s)	360, 378, 585, 586, 587, 1711, 2690, 2915
BseMI	GCAATG	3 cut(s)	704, 1692, 2257
BseMII	CTCAG	9 cut(s)	1242, 1305, 1496, 1610, 1665, 1909, 2202, 2318, 2764
BseNI	ACTGG	3 cut(s)	1887, 2386, 2926
BseRI	GAGGAG	2 cut(s)	1683, 1787
BseXI	GCAGC	8 cut(s)	247, 394, 612, 2083, 2110, 2177, 2268, 2980
BseYI	CCCAGC	1 cut(s)	131
BsgI	GTGCAG	4 cut(s)	471, 671, 932, 2184
Bsh1236I	CGCG	1 cut(s)	258
BshFI	GGCC	4 cut(s)	274, 800, 820, 1691
BshTI	ACCGGT	1 cut(s)	140
BsiHKAI	GWGCWC	2 cut(s)	1939, 2541
BsiHKCI	CYCGRG	1 cut(s)	1277
BsiSI	CCGG	6 cut(s)	141, 305, 664, 801, 889, 1080
BslFI	GGGAC	5 cut(s)	25, 440, 2018, 2694, 2932
BslI	CCNNNNNNNGG	8 cut(s)	360, 378, 585, 586, 587, 1711, 2690, 2915
BsmAI	GTCTC	5 cut(s)	69, 753, 1048, 1592, 1786
BsmFI	GGGAC	5 cut(s)	25, 440, 2018, 2694, 2932
BsmI	GAATGC	1 cut(s)	2606
BsnI	GGCC	4 cut(s)	274, 800, 820, 1691
Bso31I	GGTCTC	1 cut(s)	753
BsoBI	CYCGRG	1 cut(s)	1277
Bsp119I	TTCGAA	1 cut(s)	117
Bsp1286I	GDGCHC	3 cut(s)	1939, 1975, 2541
Bsp13I	TCCGGA	1 cut(s)	1079
Bsp19I	CCATGG	1 cut(s)	2583
BspACI	CCGC	7 cut(s)	256, 1060, 1113, 1356, 1882, 2394, 2593
BspANI	GGCC	4 cut(s)	274, 800, 820, 1691
BspCNI	CTCAG	9 cut(s)	1243, 1306, 1497, 1611, 1666, 1908, 2203, 2317, 2763
BspEI	TCCGGA	1 cut(s)	1079
BspFNI	CGCG	1 cut(s)	258
BspHI	TCATGA	4 cut(s)	751, 1768, 2821, 2866
BspLI	GGNNCC	3 cut(s)	1111, 1354, 2682
BspMAI	CTGCAG	1 cut(s)	2095
BspMI	ACCTGC	4 cut(s)	823, 1531, 2254, 2993
BspPI	GGATC	9 cut(s)	94, 296, 427, 986, 1090, 1347, 1360, 1953, 2536
BspT104I	TTCGAA	1 cut(s)	117
BspTNI	GGTCTC	1 cut(s)	753
BsrBI	CCGCTC	1 cut(s)	1884
BsrDI	GCAATG	3 cut(s)	704, 1692, 2257
BsrFI	RCCGGY	2 cut(s)	140, 663
BsrI	ACTGG	3 cut(s)	1887, 2386, 2926
BssAI	RCCGGY	2 cut(s)	140, 663
BssECI	CCNNGG	8 cut(s)	103, 583, 614, 889, 1692, 2225, 2583, 2927
BssNI	GRCGYC	1 cut(s)	344
BssSI	CACGAG	1 cut(s)	151
BssT1I	CCWWGG	3 cut(s)	583, 614, 2583
Bst2BI	CACGAG	1 cut(s)	151
Bst2UI	CCWGG	6 cut(s)	69, 105, 628, 1001, 2227, 2928
Bst6I	CTCTTC	2 cut(s)	1359, 2757
BstACI	GRCGYC	1 cut(s)	344
BstAPI	GCANNNNNTGC	1 cut(s)	1430
BstBAI	YACGTR	2 cut(s)	910, 1331
BstBI	TTCGAA	1 cut(s)	117
BstC8I	GCNNGC	6 cut(s)	398, 665, 1511, 1833, 2355, 2955
BstDSI	CCRYGG	2 cut(s)	1692, 2583
BstENI	CCTNNNNNAGG	1 cut(s)	2688
BstF5I	GGATG	6 cut(s)	575, 775, 1435, 2262, 2461, 2800
BstFNI	CGCG	1 cut(s)	258
BstH2I	RGCGCY	1 cut(s)	2780
BstHHI	GCGC	2 cut(s)	260, 2779
BstMAI	GTCTC	5 cut(s)	69, 753, 1048, 1592, 1786
BstMWI	GCNNNNNNNGC	6 cut(s)	606, 817, 1430, 1726, 1881, 2999
BstNI	CCWGG	6 cut(s)	69, 105, 628, 1001, 2227, 2928
BstNSI	RCATGY	2 cut(s)	832, 2148
BstSCI	CCNGG	8 cut(s)	67, 103, 626, 800, 888, 999, 2225, 2926
BstSFI	CTRYAG	5 cut(s)	490, 1116, 1212, 2023, 2091
BstUI	CGCG	1 cut(s)	258
BstV1I	GCAGC	8 cut(s)	247, 394, 612, 2083, 2110, 2177, 2268, 2980
BstV2I	GAAGAC	2 cut(s)	371, 1041
BstX2I	RGATCY	7 cut(s)	99, 419, 991, 1352, 1945, 2528, 2806
BstYI	RGATCY	7 cut(s)	99, 419, 991, 1352, 1945, 2528, 2806
BsuI	GTATCC	3 cut(s)	1132, 1708, 2211
BsuRI	GGCC	4 cut(s)	274, 800, 820, 1691
BtgI	CCRYGG	2 cut(s)	1692, 2583
BtrI	CACGTC	1 cut(s)	1469
BtsCI	GGATG	6 cut(s)	575, 775, 1435, 2262, 2461, 2800
BtsIMutI	CAGTG	3 cut(s)	87, 601, 1894
BveI	ACCTGC	4 cut(s)	823, 1531, 2254, 2993
Cac8I	GCNNGC	6 cut(s)	398, 665, 1511, 1833, 2355, 2955
CaiI	CAGNNNCTG	2 cut(s)	458, 2909
CciI	TCATGA	4 cut(s)	751, 1768, 2821, 2866
CfoI	GCGC	2 cut(s)	260, 2779
Cfr10I	RCCGGY	2 cut(s)	140, 663
Cfr13I	GGNCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
CseI	GACGC	1 cut(s)	333
CsiI	ACCWGGT	1 cut(s)	626
Csp6I	GTAC	3 cut(s)	236, 426, 2246
CspAI	ACCGGT	1 cut(s)	140
CspCI	CAANNNNNGTGG	2 cut(s)	1674, 1709
CviQI	GTAC	3 cut(s)	236, 426, 2246
DraI	TTTAAA	2 cut(s)	940, 2793
EaeI	YGGCCR	2 cut(s)	798, 1689
Eam1104I	CTCTTC	2 cut(s)	1359, 2757
EarI	CTCTTC	2 cut(s)	1359, 2757
Ecl136II	GAGCTC	1 cut(s)	2539
Eco130I	CCWWGG	3 cut(s)	583, 614, 2583
Eco24I	GRGCYC	2 cut(s)	1975, 2541
Eco31I	GGTCTC	1 cut(s)	753
Eco47I	GGWCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
Eco47III	AGCGCT	1 cut(s)	2778
Eco53kI	GAGCTC	1 cut(s)	2539
Eco57I	CTGAAG	6 cut(s)	1440, 2034, 2379, 2832, 2838, 2931
Eco88I	CYCGRG	1 cut(s)	1277
EcoICRI	GAGCTC	1 cut(s)	2539
EcoNI	CCTNNNNNAGG	1 cut(s)	2688
EcoO109I	RGGNCCY	2 cut(s)	2681, 3005
EcoRII	CCWGG	6 cut(s)	67, 103, 626, 999, 2225, 2926
EcoT14I	CCWWGG	3 cut(s)	583, 614, 2583
EcoT22I	ATGCAT	3 cut(s)	1552, 2146, 2670
EcoT38I	GRGCYC	2 cut(s)	1975, 2541
ErhI	CCWWGG	3 cut(s)	583, 614, 2583
FalI	AAGNNNNNCTT	6 cut(s)	2352, 2384, 2391, 2423, 2817, 2849
FaqI	GGGAC	5 cut(s)	25, 440, 2018, 2694, 2932
FauI	CCCGC	2 cut(s)	263, 1053
FauNDI	CATATG	1 cut(s)	2104
FbaI	TGATCA	1 cut(s)	1005
FblI	GTMKAC	1 cut(s)	837
Fnu4HI	GCNGC	9 cut(s)	261, 383, 601, 2072, 2099, 2166, 2282, 2594, 2994
FokI	GGATG	6 cut(s)	562, 782, 1442, 2269, 2468, 2787
FriOI	GRGCYC	2 cut(s)	1975, 2541
Fsp4HI	GCNGC	9 cut(s)	261, 383, 601, 2072, 2099, 2166, 2282, 2594, 2994
FspBI	CTAG	8 cut(s)	866, 1190, 1404, 1413, 1785, 1911, 2345, 2465
GlaI	GCGC	2 cut(s)	259, 2778
GluI	GCNGC	9 cut(s)	261, 383, 601, 2072, 2099, 2166, 2282, 2594, 2994
GsaI	CCCAGC	1 cut(s)	135
GsuI	CTGGAG	5 cut(s)	966, 1455, 1763, 1870, 2096
HaeII	RGCGCY	1 cut(s)	2780
HaeIII	GGCC	4 cut(s)	274, 800, 820, 1691
HapII	CCGG	6 cut(s)	141, 305, 664, 801, 889, 1080
HgaI	GACGC	1 cut(s)	333
HhaI	GCGC	2 cut(s)	260, 2779
Hin1I	GRCGYC	1 cut(s)	344
Hin6I	GCGC	2 cut(s)	258, 2777
HinP1I	GCGC	2 cut(s)	258, 2777
HindIII	AAGCTT	2 cut(s)	1829, 2561
HpaII	CCGG	6 cut(s)	141, 305, 664, 801, 889, 1080
Hpy166II	GTNNAC	3 cut(s)	838, 1892, 2132
Hpy8I	GTNNAC	3 cut(s)	838, 1892, 2132
Hpy99I	CGWCG	2 cut(s)	82, 1980
HpyCH4IV	ACGT	3 cut(s)	909, 1330, 1468
HpyF10VI	GCNNNNNNNGC	6 cut(s)	606, 817, 1430, 1726, 1881, 2999
HpySE526I	ACGT	3 cut(s)	909, 1330, 1468
Hsp92I	GRCGYC	1 cut(s)	344
HspAI	GCGC	2 cut(s)	258, 2777
Kpn2I	TCCGGA	1 cut(s)	1079
KroI	GCCGGC	1 cut(s)	663
KroNI	GCCGGC	1 cut(s)	665
Ksp22I	TGATCA	1 cut(s)	1005
LmnI	GCTCC	4 cut(s)	1405, 1670, 1774, 1889
Lsp1109I	GCAGC	8 cut(s)	247, 394, 612, 2083, 2110, 2177, 2268, 2980
MabI	ACCWGGT	1 cut(s)	626
MaeI	CTAG	8 cut(s)	866, 1190, 1404, 1413, 1785, 1911, 2345, 2465
MaeII	ACGT	3 cut(s)	909, 1330, 1468
MaeIII	GTNAC	8 cut(s)	242, 387, 590, 853, 2389, 2411, 2630, 2699
MbiI	CCGCTC	1 cut(s)	1884
MfeI	CAATTG	2 cut(s)	1629, 2997
MflI	RGATCY	7 cut(s)	99, 419, 991, 1352, 1945, 2528, 2806
MhlI	GDGCHC	3 cut(s)	1939, 1975, 2541
MlyI	GAGTC	4 cut(s)	36, 361, 1887, 2462
MmeI	TCCRAC	1 cut(s)	1958
Mph1103I	ATGCAT	3 cut(s)	1552, 2146, 2670
MroI	TCCGGA	1 cut(s)	1079
MroNI	GCCGGC	1 cut(s)	663
MroXI	GAANNNNTTC	3 cut(s)	1075, 2766, 2829
MseI	TTAA	8 cut(s)	171, 432, 939, 2219, 2291, 2637, 2792, 3023
MslI	CAYNNNNRTG	3 cut(s)	19, 1533, 2212
MspA1I	CMGCKG	1 cut(s)	2074
MspI	CCGG	6 cut(s)	141, 305, 664, 801, 889, 1080
MspR9I	CCNGG	8 cut(s)	69, 105, 628, 802, 890, 1001, 2227, 2928
MunI	CAATTG	2 cut(s)	1629, 2997
Mva1269I	GAATGC	1 cut(s)	2606
MvaI	CCWGG	6 cut(s)	69, 105, 628, 1001, 2227, 2928
MvnI	CGCG	1 cut(s)	258
MwoI	GCNNNNNNNGC	6 cut(s)	606, 817, 1430, 1726, 1881, 2999
NaeI	GCCGGC	1 cut(s)	665
NciI	CCSGG	2 cut(s)	802, 890
NcoI	CCATGG	1 cut(s)	2583
NdeI	CATATG	1 cut(s)	2104
NgoMIV	GCCGGC	1 cut(s)	663
NlaIV	GGNNCC	3 cut(s)	1111, 1354, 2682
NmuCI	GTSAC	4 cut(s)	853, 2389, 2411, 2630
NsiI	ATGCAT	3 cut(s)	1552, 2146, 2670
NspI	RCATGY	2 cut(s)	832, 2148
NspV	TTCGAA	1 cut(s)	117
OliI	CACNNNNGTG	1 cut(s)	2212
PagI	TCATGA	4 cut(s)	751, 1768, 2821, 2866
PctI	GAATGC	1 cut(s)	2606
PdiI	GCCGGC	1 cut(s)	665
PdmI	GAANNNNTTC	3 cut(s)	1075, 2766, 2829
PfeI	GAWTC	9 cut(s)	461, 656, 813, 1393, 1490, 1843, 2110, 2642, 2977
PinAI	ACCGGT	1 cut(s)	140
PkrI	GCNGC	9 cut(s)	262, 384, 602, 2073, 2100, 2167, 2283, 2595, 2995
PleI	GAGTC	4 cut(s)	36, 361, 1887, 2462
PpsI	GAGTC	4 cut(s)	36, 361, 1887, 2462
Ppu21I	YACGTR	2 cut(s)	910, 1331
PpuMI	RGGWCCY	2 cut(s)	2681, 3005
PshBI	ATTAAT	1 cut(s)	2637
PsiI	TTATAA	1 cut(s)	1155
Psp124BI	GAGCTC	1 cut(s)	2541
Psp5II	RGGWCCY	2 cut(s)	2681, 3005
Psp6I	CCWGG	6 cut(s)	67, 103, 626, 999, 2225, 2926
PspFI	CCCAGC	1 cut(s)	131
PspGI	CCWGG	6 cut(s)	67, 103, 626, 999, 2225, 2926
PspN4I	GGNNCC	3 cut(s)	1111, 1354, 2682
PspPI	GGNCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
PspPPI	RGGWCCY	2 cut(s)	2681, 3005
PstI	CTGCAG	1 cut(s)	2095
PstNI	CAGNNNCTG	2 cut(s)	458, 2909
PsuI	RGATCY	7 cut(s)	99, 419, 991, 1352, 1945, 2528, 2806
PvuII	CAGCTG	1 cut(s)	2074
RsaI	GTAC	3 cut(s)	237, 427, 2247
RsaNI	GTAC	3 cut(s)	236, 426, 2246
RseI	CAYNNNNRTG	3 cut(s)	19, 1533, 2212
SacI	GAGCTC	1 cut(s)	2541
SaqAI	TTAA	8 cut(s)	171, 432, 939, 2219, 2291, 2637, 2792, 3023
SatI	GCNGC	9 cut(s)	261, 383, 601, 2072, 2099, 2166, 2282, 2594, 2994
Sau96I	GGNCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
SchI	GAGTC	4 cut(s)	36, 361, 1887, 2462
ScrFI	CCNGG	8 cut(s)	69, 105, 628, 802, 890, 1001, 2227, 2928
SduI	GDGCHC	3 cut(s)	1939, 1975, 2541
SexAI	ACCWGGT	1 cut(s)	626
SfcI	CTRYAG	5 cut(s)	490, 1116, 1212, 2023, 2091
SfuI	TTCGAA	1 cut(s)	117
SinI	GGWCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
SmiMI	CAYNNNNRTG	3 cut(s)	19, 1533, 2212
SmlI	CTYRAG	4 cut(s)	95, 966, 2369, 2883
SmoI	CTYRAG	4 cut(s)	95, 966, 2369, 2883
SsiI	CCGC	7 cut(s)	256, 1060, 1113, 1356, 1882, 2394, 2593
SspI	AATATT	1 cut(s)	2787
SspMI	CTAG	8 cut(s)	866, 1190, 1404, 1413, 1785, 1911, 2345, 2465
SstI	GAGCTC	1 cut(s)	2541
StyD4I	CCNGG	8 cut(s)	67, 103, 626, 800, 888, 999, 2225, 2926
StyI	CCWWGG	3 cut(s)	583, 614, 2583
TaiI	ACGT	3 cut(s)	912, 1333, 1471
TaqI	TCGA	2 cut(s)	77, 117
TatI	WGTACW	2 cut(s)	235, 425
TauI	GCSGC	1 cut(s)	2596
TfiI	GAWTC	9 cut(s)	461, 656, 813, 1393, 1490, 1843, 2110, 2642, 2977
Tru1I	TTAA	8 cut(s)	171, 432, 939, 2219, 2291, 2637, 2792, 3023
Tru9I	TTAA	8 cut(s)	171, 432, 939, 2219, 2291, 2637, 2792, 3023
TscAI	CASTG	3 cut(s)	94, 601, 1894
TseFI	GTSAC	4 cut(s)	853, 2389, 2411, 2630
TseI	GCWGC	8 cut(s)	260, 382, 600, 2071, 2098, 2165, 2281, 2993
Tsp45I	GTSAC	4 cut(s)	853, 2389, 2411, 2630
TspGWI	ACGGA	2 cut(s)	1727, 2150
TspRI	CASTG	3 cut(s)	94, 601, 1894
VpaK11BI	GGWCC	6 cut(s)	138, 886, 2116, 2681, 2924, 3005
VspI	ATTAAT	1 cut(s)	2637
XagI	CCTNNNNNAGG	1 cut(s)	2688
XapI	RAATTY	4 cut(s)	1157, 1736, 2515, 2550
XbaI	TCTAGA	3 cut(s)	1189, 1784, 2344
XceI	RCATGY	2 cut(s)	832, 2148
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2811
XmiI	GTMKAC	1 cut(s)	837
XmnI	GAANNNNTTC	3 cut(s)	1075, 2766, 2829
XspI	CTAG	8 cut(s)	866, 1190, 1404, 1413, 1785, 1911, 2345, 2465
Zsp2I	ATGCAT	3 cut(s)	1552, 2146, 2670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.