MD17G1154200.v1.1

Remorin, C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009998
Physical Location & Seq
Forward (+)
14576328 .. 14579927
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1154200.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGATCTCACAAGCCCCAGATGCTTTCAACCTCCATCTTTTATCTCATCCAGTGGGGTAAATATCCCACATTTGGTTTTTTCCTATTACCTTTATTTTGTTTTTACATTTGCCCATAAGGGTTTTAATTACTCTTCCTTGTTTACCTTTTTCTTCCTGTTGTTTTTGGTGTTTGTGATTCAGGAACCGCCATTGGAGAGCAACCCAAGCTTCTATGGAAAAACCAGTAACAACCCATTTGCTGACACATTTCCAGACCCTCTCTGCAAGCTCAACCTAAAGGAAACCTCTGATTTTGTCAAGTCACTCCCAGTCCCAAGCAATGTCACGTCAGCACAGCGGAGGGAGGGAGGAGTGAGCTCTGTGACACAAAGGAGAGCAGTAGAAGCTCCTCCCACTCCTGGAAGGCCAGTTTTCAGCTTCTCAGTTGGCAACTTTTCGAGAAAGAGCTTTCCTTCAAAGTGGGATGATGCTGAGAAGTGGCTCATAAGCACTTCCTGCCATGAATCTCCTGCTCACACCATCAAGCCACAATCAGATTCCACAAATATTTTTAACCCAAAACAATGTGAGGTTTTTGCTGAAAAATCCAGGGTCACAGAGGATAAGGTCTCAAAGGTGGTCTCAAGCTTCCAAAGATGTGTTTCTCTGAACAATTATCGATCTGCCAGAGCTTTCAATGGGGTCTCAACCTCAGCTGATGTGCTTCTAAAAGATAAGTTTGTCAACGACATAGAACCAATTTTACCCAATTCCAGGTACTTAGAGCCAACTAAAGAAGGCTTTCTATTCAAAAACTCGGCCTGTGATGCCATAAAAGATGCAGGCAGCGGCACAGAAGAGGCTCATCATGTACACCATCGAGATGTCGGCACAGAGATGACTCCTCTTGGAAGCTCCACAACTTCGAGGTGCCCTACGCCGTTCAAGTGCTCATCTCCCGCGCGCCACAACACGCCTGCAAATAGGTCAGGGTCATTGGCAATGGGGCACTCTAGCAGCACCAACAACACCATTGACATTGCCCAGCTGCAGGAGTGCCTCGGGACACAGTACGATTCAGTTCAATCGAACTGGAGCTCAAGGGAAGAAGAGGAAGTGGAAATATCGAAGAGCTTGAGACATTTCGAAACAAGCAATCCGTGGGGAGAGAGAATTTCTGAAATTAGAGCTGCTGCATGGGAAGGGGAAGAAAATAACAAATGCTGCCTTAGGTATCAGAGAGAAGAAGCAAAAATCCAAGCTTGGGTCAACCTCCAAAGTGCAAAAGCAGAAGCTCAGTCAAGAAAGCTTGAGGTGAAAATACAGAAGATGAGATCAAACCTGGAGGAGAAGCTGATGACGAGGATGGCAGTAGTGCATAGAAAAGCTGAGGAGTGGAGAGCAACAGCGAGGCAGCAGCACTCTGAACAAATCCACAAGGGCACCAACAATGCACACAAAATGATCACAAATAGGCACAACCCGCATTTTTCTGGCCACATTTCTTGCGGCTGCTTCCCTTGCAGTAACATTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Remorin_C

Protein Analysis

507

Amino Acids

56.94

Weight (kDa)

8.65

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Remorin_C PF03763 388 - 489 2.7e-25 Remorin, C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 912, 1424
AccII CGCG 2 cut(s) 944, 946
AciI CCGC 6 cut(s) 188, 340, 831, 942, 1466, 1491
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 1477
AcsI RAATTY 1 cut(s) 1155
AfaI GTAC 3 cut(s) 761, 855, 1055
AfiI CCNNNNNNNGG 5 cut(s) 73, 401, 756, 1033, 1246
AgsI TTSAA 6 cut(s) 29, 459, 679, 793, 928, 1067
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 4 cut(s) 400, 590, 755, 1323
Alw21I GWGCWC 3 cut(s) 362, 935, 1082
Alw26I GTCTC 4 cut(s) 616, 628, 691, 1114
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 1043
AoxI GGCC 3 cut(s) 407, 801, 1477
ApeKI GCWGC 9 cut(s) 828, 999, 1030, 1172, 1175, 1206, 1396, 1399, 1494
ApoI RAATTY 1 cut(s) 1155
Asp700I GAANNNNTTC 1 cut(s) 783
AspLEI GCGC 2 cut(s) 946, 948
AsuHPI GGTGA 1 cut(s) 1309
AsuII TTCGAA 1 cut(s) 1128
AvaI CYCGRG 1 cut(s) 1043
AxyI CCTNAGG 1 cut(s) 1211
BaeGI GKGCMC 3 cut(s) 917, 993, 1427
BalI TGGCCA 1 cut(s) 1479
BanI GGYRCC 2 cut(s) 912, 1424
BanII GRGCYC 2 cut(s) 362, 1082
Bbv12I GWGCWC 3 cut(s) 362, 935, 1082
BbvCI CCTCAGC 2 cut(s) 694, 1371
BbvI GCAGC 9 cut(s) 840, 1011, 1017, 1159, 1162, 1193, 1408, 1411, 1481
BccI CCATC 4 cut(s) 43, 530, 867, 1342
BceAI ACGGC 1 cut(s) 907
BciT130I CCWGG 4 cut(s) 402, 592, 757, 1325
BclI TGATCA 1 cut(s) 1446
BcoDI GTCTC 4 cut(s) 616, 628, 691, 1114
BfaI CTAG 1 cut(s) 996
BfmI CTRYAG 1 cut(s) 1031
Bme1390I CCNGG 4 cut(s) 402, 592, 757, 1325
BmeT110I CYCGRG 1 cut(s) 1043
BmgBI CACGTC 1 cut(s) 330
BmiI GGNNCC 3 cut(s) 186, 914, 1426
BmrFI CCNGG 4 cut(s) 402, 592, 757, 1325
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 4 cut(s) 11, 460, 799, 811
BmuI ACTGGG 1 cut(s) 305
BpmI CTGGAG 2 cut(s) 1096, 1346
Bpu10I CCTNAGC 2 cut(s) 694, 1371
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 4 cut(s) 610, 1066, 1138, 1313
Bsa29I ATCGAT 1 cut(s) 661
BsaI GGTCTC 3 cut(s) 616, 628, 691
BsaJI CCNNGG 3 cut(s) 591, 1042, 1142
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 401, 756, 1033, 1246
Bse1I ACTGG 5 cut(s) 51, 225, 311, 410, 1079
Bse21I CCTNAGG 1 cut(s) 1211
Bse3DI GCAATG 3 cut(s) 328, 990, 1020
BseBI CCWGG 4 cut(s) 402, 592, 757, 1325
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 3 cut(s) 591, 1042, 1142
BseGI GGATG 3 cut(s) 47, 472, 1353
BseLI CCNNNNNNNGG 5 cut(s) 73, 401, 756, 1033, 1246
BseMI GCAATG 3 cut(s) 328, 990, 1020
BseMII CTCAG 5 cut(s) 438, 465, 708, 1292, 1362
BseNI ACTGG 5 cut(s) 51, 225, 311, 410, 1079
BsePI GCGCGC 1 cut(s) 944
BseRI GAGGAG 5 cut(s) 366, 381, 876, 1343, 1388
BseSI GKGCMC 3 cut(s) 917, 993, 1427
BseXI GCAGC 9 cut(s) 840, 1011, 1017, 1159, 1162, 1193, 1408, 1411, 1481
BseYI CCCAGC 1 cut(s) 1026
Bsh1236I CGCG 2 cut(s) 944, 946
BshFI GGCC 3 cut(s) 409, 803, 1479
BshNI GGYRCC 2 cut(s) 912, 1424
BshVI ATCGAT 1 cut(s) 661
BsiHKAI GWGCWC 3 cut(s) 362, 935, 1082
BsiHKCI CYCGRG 1 cut(s) 1043
BslFI GGGAC 2 cut(s) 299, 1060
BslI CCNNNNNNNGG 5 cut(s) 73, 401, 756, 1033, 1246
BsmAI GTCTC 4 cut(s) 616, 628, 691, 1114
BsmFI GGGAC 2 cut(s) 299, 1060
BsnI GGCC 3 cut(s) 409, 803, 1479
Bso31I GGTCTC 3 cut(s) 616, 628, 691
BsoBI CYCGRG 1 cut(s) 1043
Bsp119I TTCGAA 1 cut(s) 1128
Bsp1286I GDGCHC 6 cut(s) 362, 917, 935, 993, 1082, 1427
Bsp1407I TGTACA 1 cut(s) 853
Bsp143I GATC 4 cut(s) 4, 662, 1316, 1446
BspACI CCGC 6 cut(s) 188, 340, 831, 942, 1466, 1491
BspANI GGCC 3 cut(s) 409, 803, 1479
BspCNI CTCAG 5 cut(s) 437, 466, 707, 1291, 1363
BspDI ATCGAT 1 cut(s) 661
BspFNI CGCG 2 cut(s) 944, 946
BspLI GGNNCC 3 cut(s) 186, 914, 1426
BspMAI CTGCAG 1 cut(s) 1035
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 1106
BspT104I TTCGAA 1 cut(s) 1128
BspT107I GGYRCC 2 cut(s) 912, 1424
BspTNI GGTCTC 3 cut(s) 616, 628, 691
BsrDI GCAATG 3 cut(s) 328, 990, 1020
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 5 cut(s) 51, 225, 311, 410, 1079
BssECI CCNNGG 3 cut(s) 591, 1042, 1142
BssHII GCGCGC 1 cut(s) 944
BssMI GATC 4 cut(s) 4, 662, 1316, 1446
Bst2UI CCWGG 4 cut(s) 402, 592, 757, 1325
Bst4CI ACNGT 1 cut(s) 1053
Bst6I CTCTTC 4 cut(s) 139, 834, 1086, 1106
BstAPI GCANNNNNTGC 1 cut(s) 498
BstAUI TGTACA 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 4 cut(s) 269, 826, 946, 960
BstDEI CTNAG 7 cut(s) 424, 474, 694, 763, 1211, 1278, 1371
BstDSI CCRYGG 1 cut(s) 1142
BstF5I GGATG 3 cut(s) 47, 472, 1353
BstFNI CGCG 2 cut(s) 944, 946
BstHHI GCGC 2 cut(s) 946, 948
BstKTI GATC 4 cut(s) 7, 665, 1319, 1449
BstMAI GTCTC 4 cut(s) 616, 628, 691, 1114
BstMBI GATC 4 cut(s) 4, 662, 1316, 1446
BstMWI GCNNNNNNNGC 7 cut(s) 21, 207, 386, 498, 809, 1465, 1503
BstNI CCWGG 4 cut(s) 402, 592, 757, 1325
BstSCI CCNGG 4 cut(s) 400, 590, 755, 1323
BstSFI CTRYAG 1 cut(s) 1031
BstSLI GKGCMC 3 cut(s) 917, 993, 1427
BstUI CGCG 2 cut(s) 944, 946
BstV1I GCAGC 9 cut(s) 840, 1011, 1017, 1159, 1162, 1193, 1408, 1411, 1481
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 661
Bsu36I CCTNAGG 1 cut(s) 1211
BsuRI GGCC 3 cut(s) 409, 803, 1479
BsuTUI ATCGAT 1 cut(s) 661
BtgI CCRYGG 1 cut(s) 1142
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 3 cut(s) 47, 472, 1353
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 4 cut(s) 269, 826, 946, 960
CfoI GCGC 2 cut(s) 946, 948
ClaI ATCGAT 1 cut(s) 661
Csp6I GTAC 3 cut(s) 760, 854, 1054
CspCI CAANNNNNGTGG 2 cut(s) 1469, 1504
CviAII CATG 3 cut(s) 503, 851, 1179
CviQI GTAC 3 cut(s) 760, 854, 1054
DdeI CTNAG 7 cut(s) 424, 474, 694, 763, 1211, 1278, 1371
DpnI GATC 4 cut(s) 6, 664, 1318, 1448
DpnII GATC 4 cut(s) 4, 662, 1316, 1446
EaeI YGGCCR 1 cut(s) 1477
Eam1104I CTCTTC 4 cut(s) 139, 834, 1086, 1106
EarI CTCTTC 4 cut(s) 139, 834, 1086, 1106
Ecl136II GAGCTC 2 cut(s) 360, 1080
Eco24I GRGCYC 2 cut(s) 362, 1082
Eco31I GGTCTC 3 cut(s) 616, 628, 691
Eco53kI GAGCTC 2 cut(s) 360, 1080
Eco81I CCTNAGG 1 cut(s) 1211
Eco88I CYCGRG 1 cut(s) 1043
EcoICRI GAGCTC 2 cut(s) 360, 1080
EcoRII CCWGG 4 cut(s) 400, 590, 755, 1323
EcoT38I GRGCYC 2 cut(s) 362, 1082
FaeI CATG 3 cut(s) 506, 854, 1182
FaiI YATR 9 cut(s) 117, 216, 488, 504, 734, 815, 852, 1180, 1362
FaqI GGGAC 2 cut(s) 299, 1060
FatI CATG 3 cut(s) 502, 850, 1178
FauI CCCGC 2 cut(s) 949, 1473
FbaI TGATCA 1 cut(s) 1446
FokI GGATG 3 cut(s) 34, 479, 1360
FriOI GRGCYC 2 cut(s) 362, 1082
FspBI CTAG 1 cut(s) 996
GlaI GCGC 2 cut(s) 945, 947
GsaI CCCAGC 1 cut(s) 1030
GsuI CTGGAG 2 cut(s) 1096, 1346
HaeIII GGCC 3 cut(s) 409, 803, 1479
HhaI GCGC 2 cut(s) 946, 948
Hin1II CATG 3 cut(s) 506, 854, 1182
Hin6I GCGC 2 cut(s) 944, 946
HinP1I GCGC 2 cut(s) 944, 946
HincII GTYRAC 2 cut(s) 727, 1252
HindII GTYRAC 2 cut(s) 727, 1252
HindIII AAGCTT 4 cut(s) 208, 628, 1242, 1289
HinfI GANTC 5 cut(s) 178, 506, 539, 883, 1058
HphI GGTGA 1 cut(s) 1309
Hpy166II GTNNAC 4 cut(s) 144, 727, 856, 1252
Hpy188I TCNGA 6 cut(s) 292, 538, 651, 1162, 1221, 1408
Hpy188III TCNNGA 6 cut(s) 182, 254, 441, 863, 1045, 1284
Hpy8I GTNNAC 4 cut(s) 144, 727, 856, 1252
HpyAV CCTTC 4 cut(s) 399, 465, 773, 1178
HpyCH4III ACNGT 1 cut(s) 1053
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 9 cut(s) 267, 824, 962, 1033, 1178, 1265, 1360, 1436, 1506
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 207, 386, 498, 809, 1465, 1503
HpyF3I CTNAG 7 cut(s) 424, 474, 694, 763, 1211, 1278, 1371
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 3 cut(s) 506, 854, 1182
HspAI GCGC 2 cut(s) 944, 946
Ksp22I TGATCA 1 cut(s) 1446
Kzo9I GATC 4 cut(s) 4, 662, 1316, 1446
LguI GCTCTTC 1 cut(s) 1106
LmnI GCTCC 3 cut(s) 394, 902, 1077
Lsp1109I GCAGC 9 cut(s) 840, 1011, 1017, 1159, 1162, 1193, 1408, 1411, 1481
LweI GCATC 4 cut(s) 11, 460, 799, 811
MaeI CTAG 1 cut(s) 996
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 6 cut(s) 227, 303, 325, 364, 595, 1508
MalI GATC 4 cut(s) 6, 664, 1318, 1448
MboI GATC 4 cut(s) 4, 662, 1316, 1446
MboII GAAGA 9 cut(s) 126, 145, 851, 1100, 1103, 1123, 1202, 1238, 1321
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 6 cut(s) 362, 917, 935, 993, 1082, 1427
MlsI TGGCCA 1 cut(s) 1479
MluCI AATT 6 cut(s) 127, 655, 741, 751, 1155, 1164
MluNI TGGCCA 1 cut(s) 1479
MlyI GAGTC 1 cut(s) 877
Mox20I TGGCCA 1 cut(s) 1479
MroXI GAANNNNTTC 1 cut(s) 783
MscI TGGCCA 1 cut(s) 1479
MseI TTAA 3 cut(s) 126, 555, 1515
MslI CAYNNNNRTG 2 cut(s) 864, 878
Msp20I TGGCCA 1 cut(s) 1479
MspA1I CMGCKG 4 cut(s) 340, 698, 831, 1030
MspR9I CCNGG 4 cut(s) 402, 592, 757, 1325
MvaI CCWGG 4 cut(s) 402, 592, 757, 1325
MvnI CGCG 2 cut(s) 944, 946
MwoI GCNNNNNNNGC 7 cut(s) 21, 207, 386, 498, 809, 1465, 1503
NdeII GATC 4 cut(s) 4, 662, 1316, 1446
NlaIII CATG 3 cut(s) 506, 854, 1182
NlaIV GGNNCC 3 cut(s) 186, 914, 1426
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 4 cut(s) 303, 325, 364, 595
NspV TTCGAA 1 cut(s) 1128
PauI GCGCGC 1 cut(s) 944
PciSI GCTCTTC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 4 cut(s) 178, 506, 539, 1058
PfoI TCCNGGA 1 cut(s) 400
PleI GAGTC 1 cut(s) 877
PpsI GAGTC 1 cut(s) 877
Psp124BI GAGCTC 2 cut(s) 362, 1082
Psp6I CCWGG 4 cut(s) 400, 590, 755, 1323
PspFI CCCAGC 1 cut(s) 1026
PspGI CCWGG 4 cut(s) 400, 590, 755, 1323
PspN4I GGNNCC 3 cut(s) 186, 914, 1426
PsrI GAACNNNNNNTAC 2 cut(s) 729, 761
PstI CTGCAG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 4
PteI GCGCGC 1 cut(s) 944
PvuII CAGCTG 2 cut(s) 698, 1030
RsaI GTAC 3 cut(s) 761, 855, 1055
RsaNI GTAC 3 cut(s) 760, 854, 1054
RseI CAYNNNNRTG 2 cut(s) 864, 878
SacI GAGCTC 2 cut(s) 362, 1082
SapI GCTCTTC 1 cut(s) 1106
SaqAI TTAA 3 cut(s) 126, 555, 1515
Sau3AI GATC 4 cut(s) 4, 662, 1316, 1446
SchI GAGTC 1 cut(s) 877
ScrFI CCNGG 4 cut(s) 402, 592, 757, 1325
SduI GDGCHC 6 cut(s) 362, 917, 935, 993, 1082, 1427
SfaNI GCATC 4 cut(s) 11, 460, 799, 811
SfcI CTRYAG 1 cut(s) 1031
SfuI TTCGAA 1 cut(s) 1128
SmiMI CAYNNNNRTG 2 cut(s) 864, 878
SmlI CTYRAG 4 cut(s) 625, 1081, 1117, 1292
SmoI CTYRAG 4 cut(s) 625, 1081, 1117, 1292
Sse9I AATT 6 cut(s) 127, 655, 741, 751, 1155, 1164
SsiI CCGC 6 cut(s) 188, 340, 831, 942, 1466, 1491
SspI AATATT 1 cut(s) 550
SspMI CTAG 1 cut(s) 996
SstI GAGCTC 2 cut(s) 362, 1082
StyD4I CCNGG 4 cut(s) 400, 590, 755, 1323
TaaI ACNGT 1 cut(s) 1053
TaiI ACGT 1 cut(s) 332
TaqI TCGA 7 cut(s) 440, 661, 862, 908, 1070, 1109, 1128
TasI AATT 6 cut(s) 127, 655, 741, 751, 1155, 1164
TatI WGTACW 1 cut(s) 853
TauI GCSGC 2 cut(s) 834, 1494
TfiI GAWTC 4 cut(s) 178, 506, 539, 1058
Tru1I TTAA 3 cut(s) 126, 555, 1515
Tru9I TTAA 3 cut(s) 126, 555, 1515
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 4 cut(s) 303, 325, 364, 595
TseI GCWGC 9 cut(s) 828, 999, 1030, 1172, 1175, 1206, 1396, 1399, 1494
Tsp45I GTSAC 4 cut(s) 303, 325, 364, 595
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 1131
TspRI CASTG 1 cut(s) 58
XapI RAATTY 1 cut(s) 1155
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 1 cut(s) 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.