Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002903

Physical Location & Seq

Genomic Coordinates Forward (+)

21557362 .. 21561270

Gene Structure 3909 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1183100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGGGGAAGAAGGCGGTGATAATCGTTACTGCGACGGCAGTGTGCGCCGCGGCAGCGGTTCTGGTGGTGCGCCACCGCATGCGGAGCTCGGGACGGTGGGCCCGTGCATCGGCGATCATCAAGGAGCTCGAGGAGAAGTGTGGGACCCCCACTGGGAAGCTGAGGCAGGTGGCTGACGCCATGGCTGTCGAGATGCATGCCGGCCTCGCCTCCGAAGGTGGCAGCAAGCTCAAGATGATCATTAGCTACGTCGACAATCTCCCTACTGGGAATGAGAAAGGGTTGTTTTATGCATTGGATCTCGGAGGAACAAACTTCCGTGTACTGAGGGTGCAATTGGGAGGAAAGGGTCGTGGAATTATTAGCCAAGAATTTACTGAGGTCTCAATTCCTGAAAATCTTATGGTCGGGACTTCAGATGCACTCTTTGACTACATTGCGGCAGAACTTGCTAAGTTTGTTGCTAAAGAAGGTCAAGATTATCAACTCCCTCCTGGCAGGCAGAGAGAACTAGGTTTTACCTTCTCATTCCCTGTGCTGCAATCATCAATTAATTCGGGGACCCTTATTAAGTGGACGAAAGGCTTTTCTATAGATGATGCAGTTGGGCAAGATGTAGTGGCTGAATTGAGTAAAGCCATTGAAAGAACAGGCCTCGATATGCGTGTATCTGCTCTGGTTAATGACACGGTTGGGACATTAGCTGGAGGCAGGTATGTCAATCAGGATGTTGTTGTTGCTGTGATTTTAGGTACTGGGACAAATGCAGCATATGTGGAACGTGCACATGCAATACCAAAATGGCATGGTCTGCTCCCTAAATCAGGAGAGATGGTTGTCAACATGGAGTGGGGTAACTTTAAGTCAGCTCACCTTCCATTGACAGAATATGATCACTCATTGGATACTGAAAGTTTGAACCGTGGTGATCAGATTTTTGAGAAGATAATTTCTGGAATGTACTTGGGAGAAATTGTTCGCAGAGTTCTTTGTAGGATTGCTGAAGAAGCTTCCCTTTTTGGTGACACTGTTCCACCAAAGTTAAAAGTTCCTTTCATTTTGAGGACACCTGACATGTCTGCGATGCATCACGATGCATCCTCTGATCTTAGGGTGGTACGAGAAAAATTGAAGAATGTATTGGAGATATCAAATACTTCTCTTAAAGTAAGAAAAGTTATTGTCGAGCTCTGCAATATAGTTGCTACACGTGGGGCCCGCCTTGCCGCTGCTGGAGTCTTGGGTGTACTGAAAAAGCTGGGAAGAGACGCGGTCAAGGATGGGGAAAACCAGAAGACAGTGGTAGCTTTGGATGGTGGATTATACGAGCATTACACTGAATATAGCAAGTGCATGGAAAATACTCTACGAGAATTGCTCGGAGAGGAAGTTGCAGAAACTATCGTTATTGAACACTCTAATGATGGCTCTGGGATTGGAGCTGCCCTTCTCGCCGCCTCTCACTCACAATACCTAGGAATCGATGAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230

Pfam Domains

Protein Families

Hexokinase_1 Hexokinase_2

Protein Analysis

498

Amino Acids

53.76

Weight (kDa)

6.3

Isoelectric Point (pI)

28.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hexokinase_1	PF00349	40 - 239	7.1e-74	Hexokinase
Hexokinase_2	PF03727	246 - 486	4.1e-83	Hexokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37840
fragaria_vesca	FvH4_1g18730 FvH4_6g32470 FvH4_6g32470 FvH4_6g32470 FvH4_6g32470
malus_domestica	MD02G1194700.v1.1 MD09G1202200.v1.1 MD15G1305100.v1.1 MD17G1183100.v1.1
prunus_persica	Prupe.3G057800_v2.0.a1 Prupe.6G212100_v2.0.a1
pyrus_communis	pycom07g16990 pycom09g11900 pycom09g11910 pycom15g26750 pycom17g19210
rosa_chinensis	RchiOBHm_Chr2g0109591 RchiOBHm_Chr2g0140711
rosa_laevigata	RLG00000017748 RLG00000019894
rosa_multiflora	Rmu_sc0000332.1_g000047 Rmu_sc0003342.1_g000014
rosa_roxburghii	Rroxscaffold_2G00134120
rosa_rugosa	Rorug02G0160900 Rorug02G0161000 Rorug02G0364300 Rorug02G0364400 Rorug02G0364500
rosa_samantha	Rh2AG213300 Rh2AG416000 Rh2BG223500 Rh2BG425600 Rh2CG215300 Rh2CG402200 Rh2DG218800 Rh2DG435500
rosa_wichuraiana	Rw2G034040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	157
Acc36I	ACCTGC	2 cut(s)	157, 702
AccI	GTMKAC	1 cut(s)	252
AccII	CGCG	2 cut(s)	50, 1271
AclWI	GGATC	1 cut(s)	306
AcsI	RAATTY	1 cut(s)	371
AcuI	CTGAAG	2 cut(s)	399, 1023
AcvI	CACGTG	1 cut(s)	1211
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	5 cut(s)	324, 754, 962, 1119, 1248
AfiI	CCNNNNNNNGG	3 cut(s)	109, 153, 824
AflIII	ACRYGT	2 cut(s)	1074, 1208
AgsI	TTSAA	4 cut(s)	644, 919, 1132, 1412
AjnI	CCWGG	1 cut(s)	493
AjuI	GAANNNNNNNTTGG	2 cut(s)	1124, 1156
AloI	GAACNNNNNNTCC	2 cut(s)	960, 992
Alw21I	GWGCWC	4 cut(s)	89, 129, 787, 1191
Alw26I	GTCTC	2 cut(s)	388, 1260
Alw44I	GTGCAC	1 cut(s)	783
AlwI	GGATC	1 cut(s)	306
Ama87I	CYCGRG	2 cut(s)	88, 128
AoxI	GGCC	4 cut(s)	99, 202, 652, 1215
ApaI	GGGCCC	2 cut(s)	103, 1219
ApaLI	GTGCAC	1 cut(s)	783
ApeKI	GCWGC	6 cut(s)	53, 222, 538, 767, 1229, 1442
ApoI	RAATTY	1 cut(s)	371
AseI	ATTAAT	1 cut(s)	552
Asp700I	GAANNNNTTC	1 cut(s)	975
AspA2I	CCTAGG	1 cut(s)	1474
AspLEI	GCGC	2 cut(s)	47, 72
AspS9I	GGNCC	6 cut(s)	99, 100, 144, 561, 1215, 1216
AsuHPI	GGTGA	4 cut(s)	28, 863, 938, 1034
AvaI	CYCGRG	2 cut(s)	88, 128
AvaII	GGWCC	2 cut(s)	144, 561
AvrII	CCTAGG	1 cut(s)	1474
BaeGI	GKGCMC	3 cut(s)	103, 787, 1219
BanII	GRGCYC	5 cut(s)	89, 103, 129, 1191, 1219
BbrPI	CACGTG	1 cut(s)	1211
BbsI	GAAGAC	1 cut(s)	1301
Bbv12I	GWGCWC	4 cut(s)	89, 129, 787, 1191
BbvCI	CCTCAGC	1 cut(s)	161
BbvI	GCAGC	6 cut(s)	65, 234, 525, 779, 1216, 1429
BccI	CCATC	4 cut(s)	826, 1274, 1307, 1418
BceAI	ACGGC	1 cut(s)	51
BcgI	CGANNNNNNTGC	2 cut(s)	179, 213
BciT130I	CCWGG	1 cut(s)	495
BciVI	GTATCC	1 cut(s)	898
BclI	TGATCA	3 cut(s)	237, 892, 928
BcoDI	GTCTC	2 cut(s)	388, 1260
BfaI	CTAG	2 cut(s)	512, 1475
BfmI	CTRYAG	1 cut(s)	591
BfuAI	ACCTGC	2 cut(s)	157, 702
BfuI	GTATCC	1 cut(s)	898
BlnI	CCTAGG	1 cut(s)	1474
Bme1390I	CCNGG	1 cut(s)	495
Bme18I	GGWCC	2 cut(s)	144, 561
BmeT110I	CYCGRG	2 cut(s)	88, 128
BmgT120I	GGNCC	6 cut(s)	99, 100, 144, 561, 1215, 1216
BmiI	GGNNCC	7 cut(s)	101, 145, 146, 562, 563, 1216, 1217
BmrFI	CCNGG	1 cut(s)	495
BmrI	ACTGGG	3 cut(s)	162, 276, 765
BmsI	GCATC	8 cut(s)	116, 183, 409, 589, 1074, 1084, 1096, 1106
BmuI	ACTGGG	3 cut(s)	162, 276, 765
BpiI	GAAGAC	1 cut(s)	1301
BplI	GAGNNNNNCTC	2 cut(s)	1362, 1394
BpmI	CTGGAG	2 cut(s)	726, 1254
Bpu10I	CCTNAGC	1 cut(s)	161
BpuEI	CTTGAG	1 cut(s)	215
Bsa29I	ATCGAT	1 cut(s)	1482
BsaAI	YACGTR	1 cut(s)	1211
BsaHI	GRCGYC	1 cut(s)	177
BsaI	GGTCTC	1 cut(s)	388
BsaJI	CCNNGG	4 cut(s)	48, 180, 922, 1474
BsaXI	ACNNNNNCTCC	6 cut(s)	819, 849, 960, 990, 1374, 1404
Bsc4I	CCNNNNNNNGG	3 cut(s)	109, 153, 824
Bse118I	RCCGGY	1 cut(s)	200
Bse1I	ACTGG	3 cut(s)	157, 271, 760
Bse3DI	GCAATG	1 cut(s)	435
BseBI	CCWGG	1 cut(s)	495
BseCI	ATCGAT	1 cut(s)	1482
BseDI	CCNNGG	4 cut(s)	48, 180, 922, 1474
BseGI	GGATG	4 cut(s)	733, 1097, 1285, 1318
BseLI	CCNNNNNNNGG	3 cut(s)	109, 153, 824
BseMI	GCAATG	1 cut(s)	435
BseMII	CTCAG	3 cut(s)	152, 317, 369
BseNI	ACTGG	3 cut(s)	157, 271, 760
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	3 cut(s)	103, 787, 1219
BseXI	GCAGC	6 cut(s)	65, 234, 525, 779, 1216, 1429
BseYI	CCCAGC	1 cut(s)	1258
Bsh1236I	CGCG	2 cut(s)	50, 1271
BshFI	GGCC	4 cut(s)	101, 204, 654, 1217
BshVI	ATCGAT	1 cut(s)	1482
BsiHKAI	GWGCWC	4 cut(s)	89, 129, 787, 1191
BsiHKCI	CYCGRG	2 cut(s)	88, 128
BsiSI	CCGG	1 cut(s)	201
BslFI	GGGAC	6 cut(s)	105, 157, 424, 574, 709, 772
BslI	CCNNNNNNNGG	3 cut(s)	109, 153, 824
BsmAI	GTCTC	2 cut(s)	388, 1260
BsmBI	CGTCTC	1 cut(s)	1260
BsmFI	GGGAC	6 cut(s)	105, 157, 424, 574, 709, 772
BsnI	GGCC	4 cut(s)	101, 204, 654, 1217
Bso31I	GGTCTC	1 cut(s)	388
BsoBI	CYCGRG	2 cut(s)	88, 128
Bsp120I	GGGCCC	2 cut(s)	99, 1215
Bsp1286I	GDGCHC	6 cut(s)	89, 103, 129, 787, 1191, 1219
Bsp143I	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
Bsp19I	CCATGG	1 cut(s)	180
BspANI	GGCC	4 cut(s)	101, 204, 654, 1217
BspCNI	CTCAG	3 cut(s)	153, 318, 370
BspDI	ATCGAT	1 cut(s)	1482
BspFNI	CGCG	2 cut(s)	50, 1271
BspLI	GGNNCC	7 cut(s)	101, 145, 146, 562, 563, 1216, 1217
BspMI	ACCTGC	2 cut(s)	157, 702
BspPI	GGATC	1 cut(s)	306
BspTNI	GGTCTC	1 cut(s)	388
BsrDI	GCAATG	1 cut(s)	435
BsrFI	RCCGGY	1 cut(s)	200
BsrI	ACTGG	3 cut(s)	157, 271, 760
BssAI	RCCGGY	1 cut(s)	200
BssECI	CCNNGG	4 cut(s)	48, 180, 922, 1474
BssMI	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
BssNI	GRCGYC	1 cut(s)	177
BssT1I	CCWWGG	2 cut(s)	180, 1474
Bst2UI	CCWGG	1 cut(s)	495
Bst4CI	ACNGT	5 cut(s)	96, 691, 923, 1030, 1300
Bst6I	CTCTTC	1 cut(s)	1258
BstACI	GRCGYC	1 cut(s)	177
BstAPI	GCANNNNNTGC	2 cut(s)	449, 811
BstBAI	YACGTR	1 cut(s)	1211
BstC8I	GCNNGC	6 cut(s)	80, 198, 202, 227, 500, 1219
BstDEI	CTNAG	5 cut(s)	161, 326, 378, 453, 1109
BstDSI	CCRYGG	3 cut(s)	48, 180, 922
BstENI	CCTNNNNNAGG	1 cut(s)	822
BstF5I	GGATG	4 cut(s)	733, 1097, 1285, 1318
BstFNI	CGCG	2 cut(s)	50, 1271
BstHHI	GCGC	2 cut(s)	47, 72
BstKTI	GATC	6 cut(s)	117, 240, 301, 895, 931, 1108
BstMAI	GTCTC	2 cut(s)	388, 1260
BstMBI	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
BstMWI	GCNNNNNNNGC	9 cut(s)	44, 53, 84, 206, 449, 811, 1007, 1223, 1451
BstNI	CCWGG	1 cut(s)	495
BstNSI	RCATGY	4 cut(s)	82, 200, 791, 1078
BstSCI	CCNGG	1 cut(s)	493
BstSFI	CTRYAG	1 cut(s)	591
BstSLI	GKGCMC	3 cut(s)	103, 787, 1219
BstUI	CGCG	2 cut(s)	50, 1271
BstV1I	GCAGC	6 cut(s)	65, 234, 525, 779, 1216, 1429
BstV2I	GAAGAC	1 cut(s)	1301
BstX2I	RGATCY	1 cut(s)	298
BstYI	RGATCY	1 cut(s)	298
Bsu15I	ATCGAT	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	898
BsuRI	GGCC	4 cut(s)	101, 204, 654, 1217
BsuTUI	ATCGAT	1 cut(s)	1482
BtgI	CCRYGG	3 cut(s)	48, 180, 922
BtgZI	GCGATG	1 cut(s)	1097
BtsCI	GGATG	4 cut(s)	733, 1097, 1285, 1318
BtsI	GCAGTG	1 cut(s)	45
BtsIMutI	CAGTG	5 cut(s)	45, 150, 1026, 1305, 1335
BveI	ACCTGC	2 cut(s)	157, 702
Cac8I	GCNNGC	6 cut(s)	80, 198, 202, 227, 500, 1219
CfoI	GCGC	2 cut(s)	47, 72
Cfr10I	RCCGGY	1 cut(s)	200
Cfr13I	GGNCC	6 cut(s)	99, 100, 144, 561, 1215, 1216
Cfr42I	CCGCGG	1 cut(s)	51
ClaI	ATCGAT	1 cut(s)	1482
CseI	GACGC	2 cut(s)	185, 1277
Csp6I	GTAC	5 cut(s)	323, 753, 961, 1118, 1247
CviAII	CATG	8 cut(s)	79, 181, 197, 788, 806, 844, 1075, 1354
CviQI	GTAC	5 cut(s)	323, 753, 961, 1118, 1247
DdeI	CTNAG	5 cut(s)	161, 326, 378, 453, 1109
DpnI	GATC	6 cut(s)	116, 239, 300, 894, 930, 1107
DpnII	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
Eam1104I	CTCTTC	1 cut(s)	1258
EarI	CTCTTC	1 cut(s)	1258
Ecl136II	GAGCTC	3 cut(s)	87, 127, 1189
Eco130I	CCWWGG	2 cut(s)	180, 1474
Eco147I	AGGCCT	1 cut(s)	654
Eco24I	GRGCYC	5 cut(s)	89, 103, 129, 1191, 1219
Eco31I	GGTCTC	1 cut(s)	388
Eco32I	GATATC	1 cut(s)	1149
Eco47I	GGWCC	2 cut(s)	144, 561
Eco53kI	GAGCTC	3 cut(s)	87, 127, 1189
Eco57I	CTGAAG	2 cut(s)	399, 1023
Eco72I	CACGTG	1 cut(s)	1211
Eco88I	CYCGRG	2 cut(s)	88, 128
EcoICRI	GAGCTC	3 cut(s)	87, 127, 1189
EcoNI	CCTNNNNNAGG	1 cut(s)	822
EcoO109I	RGGNCCY	3 cut(s)	144, 561, 1215
EcoRII	CCWGG	1 cut(s)	493
EcoRV	GATATC	1 cut(s)	1149
EcoT14I	CCWWGG	2 cut(s)	180, 1474
EcoT22I	ATGCAT	4 cut(s)	198, 295, 1089, 1099
EcoT38I	GRGCYC	5 cut(s)	89, 103, 129, 1191, 1219
ErhI	CCWWGG	2 cut(s)	180, 1474
Esp3I	CGTCTC	1 cut(s)	1260
FaeI	CATG	8 cut(s)	82, 184, 200, 791, 809, 847, 1078, 1357
FalI	AAGNNNNNCTT	2 cut(s)	999, 1031
FaqI	GGGAC	6 cut(s)	105, 157, 424, 574, 709, 772
FatI	CATG	8 cut(s)	78, 180, 196, 787, 805, 843, 1074, 1353
FauI	CCCGC	1 cut(s)	1226
FauNDI	CATATG	1 cut(s)	772
FbaI	TGATCA	3 cut(s)	237, 892, 928
FblI	GTMKAC	1 cut(s)	252
FokI	GGATG	4 cut(s)	740, 1084, 1292, 1325
FriOI	GRGCYC	5 cut(s)	89, 103, 129, 1191, 1219
FspBI	CTAG	2 cut(s)	512, 1475
GlaI	GCGC	2 cut(s)	46, 71
GsaI	CCCAGC	1 cut(s)	1262
GsuI	CTGGAG	2 cut(s)	726, 1254
HaeIII	GGCC	4 cut(s)	101, 204, 654, 1217
HapII	CCGG	1 cut(s)	201
HgaI	GACGC	2 cut(s)	185, 1277
HhaI	GCGC	2 cut(s)	47, 72
Hin1I	GRCGYC	1 cut(s)	177
Hin1II	CATG	8 cut(s)	82, 184, 200, 791, 809, 847, 1078, 1357
Hin6I	GCGC	2 cut(s)	45, 70
HinP1I	GCGC	2 cut(s)	45, 70
HincII	GTYRAC	2 cut(s)	253, 841
HindII	GTYRAC	2 cut(s)	253, 841
HindIII	AAGCTT	1 cut(s)	1008
HinfI	GANTC	3 cut(s)	1236, 1479, 1487
HpaII	CCGG	1 cut(s)	201
HphI	GGTGA	4 cut(s)	28, 863, 938, 1034
Hpy166II	GTNNAC	6 cut(s)	253, 323, 576, 785, 841, 1247
Hpy188I	TCNGA	6 cut(s)	214, 305, 418, 933, 1105, 1382
Hpy8I	GTNNAC	6 cut(s)	253, 323, 576, 785, 841, 1247
Hpy99I	CGWCG	2 cut(s)	37, 254
HpyAV	CCTTC	6 cut(s)	4, 209, 464, 532, 884, 1457
HpyCH4III	ACNGT	5 cut(s)	96, 691, 923, 1030, 1300
HpyCH4IV	ACGT	3 cut(s)	249, 781, 1210
HpyF10VI	GCNNNNNNNGC	9 cut(s)	44, 53, 84, 206, 449, 811, 1007, 1223, 1451
HpyF3I	CTNAG	5 cut(s)	161, 326, 378, 453, 1109
HpySE526I	ACGT	3 cut(s)	249, 781, 1210
Hsp92I	GRCGYC	1 cut(s)	177
Hsp92II	CATG	8 cut(s)	82, 184, 200, 791, 809, 847, 1078, 1357
HspAI	GCGC	2 cut(s)	45, 70
KflI	GGGWCCC	2 cut(s)	144, 561
KroI	GCCGGC	1 cut(s)	200
KroNI	GCCGGC	1 cut(s)	202
Ksp22I	TGATCA	3 cut(s)	237, 892, 928
KspI	CCGCGG	1 cut(s)	51
Kzo9I	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
LmnI	GCTCC	4 cut(s)	84, 124, 819, 1439
Lsp1109I	GCAGC	6 cut(s)	65, 234, 525, 779, 1216, 1429
LweI	GCATC	8 cut(s)	116, 183, 409, 589, 1074, 1084, 1096, 1106
MaeI	CTAG	2 cut(s)	512, 1475
MaeII	ACGT	3 cut(s)	249, 781, 1210
MaeIII	GTNAC	3 cut(s)	25, 854, 1022
MalI	GATC	6 cut(s)	116, 239, 300, 894, 930, 1107
MboI	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
MboII	GAAGA	6 cut(s)	19, 955, 1016, 1144, 1275, 1306
MfeI	CAATTG	1 cut(s)	335
MflI	RGATCY	1 cut(s)	298
MhlI	GDGCHC	6 cut(s)	89, 103, 129, 787, 1191, 1219
MlyI	GAGTC	1 cut(s)	1245
Mph1103I	ATGCAT	4 cut(s)	198, 295, 1089, 1099
MroNI	GCCGGC	1 cut(s)	200
MroXI	GAANNNNTTC	1 cut(s)	975
MseI	TTAA	6 cut(s)	552, 570, 681, 861, 1043, 1164
MslI	CAYNNNNRTG	2 cut(s)	1092, 1419
MspA1I	CMGCKG	3 cut(s)	50, 56, 1229
MspI	CCGG	1 cut(s)	201
MspR9I	CCNGG	1 cut(s)	495
MunI	CAATTG	1 cut(s)	335
MvaI	CCWGG	1 cut(s)	495
MvnI	CGCG	2 cut(s)	50, 1271
MwoI	GCNNNNNNNGC	9 cut(s)	44, 53, 84, 206, 449, 811, 1007, 1223, 1451
NaeI	GCCGGC	1 cut(s)	202
NcoI	CCATGG	1 cut(s)	180
NdeI	CATATG	1 cut(s)	772
NdeII	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
NgoMIV	GCCGGC	1 cut(s)	200
NlaIII	CATG	8 cut(s)	82, 184, 200, 791, 809, 847, 1078, 1357
NlaIV	GGNNCC	7 cut(s)	101, 145, 146, 562, 563, 1216, 1217
NmuCI	GTSAC	1 cut(s)	1022
NsiI	ATGCAT	4 cut(s)	198, 295, 1089, 1099
NspI	RCATGY	4 cut(s)	82, 200, 791, 1078
PaeI	GCATGC	2 cut(s)	82, 200
PaeR7I	CTCGAG	1 cut(s)	128
PaqCI	CACCTGC	1 cut(s)	157
PceI	AGGCCT	1 cut(s)	654
PciI	ACATGT	1 cut(s)	1074
PcsI	WCGNNNNNNNCGW	1 cut(s)	100
PdiI	GCCGGC	1 cut(s)	202
PdmI	GAANNNNTTC	1 cut(s)	975
PfeI	GAWTC	2 cut(s)	1479, 1487
PflFI	GACNNNGTC	1 cut(s)	1271
PleI	GAGTC	1 cut(s)	1244
PmaCI	CACGTG	1 cut(s)	1211
PmlI	CACGTG	1 cut(s)	1211
PpsI	GAGTC	1 cut(s)	1244
Ppu21I	YACGTR	1 cut(s)	1211
PpuMI	RGGWCCY	2 cut(s)	144, 561
PscI	ACATGT	1 cut(s)	1074
PshBI	ATTAAT	1 cut(s)	552
Psp124BI	GAGCTC	3 cut(s)	89, 129, 1191
Psp5II	RGGWCCY	2 cut(s)	144, 561
Psp6I	CCWGG	1 cut(s)	493
PspCI	CACGTG	1 cut(s)	1211
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	1 cut(s)	493
PspN4I	GGNNCC	7 cut(s)	101, 145, 146, 562, 563, 1216, 1217
PspOMI	GGGCCC	2 cut(s)	99, 1215
PspPI	GGNCC	6 cut(s)	99, 100, 144, 561, 1215, 1216
PspPPI	RGGWCCY	2 cut(s)	144, 561
PspXI	VCTCGAGB	1 cut(s)	128
PsuI	RGATCY	1 cut(s)	298
PsyI	GACNNNGTC	1 cut(s)	1271
RsaI	GTAC	5 cut(s)	324, 754, 962, 1119, 1248
RsaNI	GTAC	5 cut(s)	323, 753, 961, 1118, 1247
RseI	CAYNNNNRTG	2 cut(s)	1092, 1419
SacI	GAGCTC	3 cut(s)	89, 129, 1191
SacII	CCGCGG	1 cut(s)	51
SalI	GTCGAC	1 cut(s)	251
SaqAI	TTAA	6 cut(s)	552, 570, 681, 861, 1043, 1164
Sau3AI	GATC	6 cut(s)	114, 237, 298, 892, 928, 1105
Sau96I	GGNCC	6 cut(s)	99, 100, 144, 561, 1215, 1216
SchI	GAGTC	1 cut(s)	1245
ScrFI	CCNGG	1 cut(s)	495
SduI	GDGCHC	6 cut(s)	89, 103, 129, 787, 1191, 1219
SfaNI	GCATC	8 cut(s)	116, 183, 409, 589, 1074, 1084, 1096, 1106
SfcI	CTRYAG	1 cut(s)	591
Sfr274I	CTCGAG	1 cut(s)	128
Sfr303I	CCGCGG	1 cut(s)	51
SgrBI	CCGCGG	1 cut(s)	51
SinI	GGWCC	2 cut(s)	144, 561
SlaI	CTCGAG	1 cut(s)	128
SmiMI	CAYNNNNRTG	2 cut(s)	1092, 1419
SmlI	CTYRAG	2 cut(s)	128, 230
SmoI	CTYRAG	2 cut(s)	128, 230
SphI	GCATGC	2 cut(s)	82, 200
SseBI	AGGCCT	1 cut(s)	654
SspMI	CTAG	2 cut(s)	512, 1475
SstI	GAGCTC	3 cut(s)	89, 129, 1191
StuI	AGGCCT	1 cut(s)	654
StyD4I	CCNGG	1 cut(s)	493
StyI	CCWWGG	2 cut(s)	180, 1474
TaaI	ACNGT	5 cut(s)	96, 691, 923, 1030, 1300
TaiI	ACGT	3 cut(s)	252, 784, 1213
TaqI	TCGA	6 cut(s)	129, 189, 252, 657, 1185, 1482
TatI	WGTACW	3 cut(s)	322, 960, 1246
TauI	GCSGC	5 cut(s)	50, 53, 443, 1229, 1457
TfiI	GAWTC	2 cut(s)	1479, 1487
Tru1I	TTAA	6 cut(s)	552, 570, 681, 861, 1043, 1164
Tru9I	TTAA	6 cut(s)	552, 570, 681, 861, 1043, 1164
TscAI	CASTG	5 cut(s)	45, 157, 1033, 1305, 1342
TseFI	GTSAC	1 cut(s)	1022
TseI	GCWGC	6 cut(s)	53, 222, 538, 767, 1229, 1442
Tsp45I	GTSAC	1 cut(s)	1022
TspDTI	ATGAA	1 cut(s)	1045
TspGWI	ACGGA	1 cut(s)	308
TspRI	CASTG	5 cut(s)	45, 157, 1033, 1305, 1342
Tth111I	GACNNNGTC	1 cut(s)	1271
VneI	GTGCAC	1 cut(s)	783
VpaK11BI	GGWCC	2 cut(s)	144, 561
VspI	ATTAAT	1 cut(s)	552
XagI	CCTNNNNNAGG	1 cut(s)	822
XapI	RAATTY	1 cut(s)	371
XceI	RCATGY	4 cut(s)	82, 200, 791, 1078
XhoI	CTCGAG	1 cut(s)	128
XmaJI	CCTAGG	1 cut(s)	1474
XmiI	GTMKAC	1 cut(s)	252
XmnI	GAANNNNTTC	1 cut(s)	975
XspI	CTAG	2 cut(s)	512, 1475
Zsp2I	ATGCAT	4 cut(s)	198, 295, 1089, 1099

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.