Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002903

Physical Location & Seq

Genomic Coordinates Reverse (-)

58255120 .. 58260449

Gene Structure 5330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ51112

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGGGGAAGAAGGCGGTCGTGATCATCTGCGGCGCTGCGGTGGTGACCGTGGCGGCGGCTCTGGTGGTCCGTCAGCGCATGCGGTGCTCCGGCCATTGGTCCAAAGCTACGGCCATCGTCAAGGAGCTGGAGGAGAAATGTGGGACCCCCATTGCCAAGCTGAGACAGGTGGCCGACGCCATGACTGTCGAGATGCACGCCGGTCTCGCCTCCGAGGGTGGAAGCAAGCTCAAGATGATCATCAGCTACGTGGATAATCTCCCCAATGGGAATGAGAAAGGGTTGTTTTATGCGTTGGACCTTGGAGGAACAAACTTCCGTGTGCTGCGGGTTCAATTGGGGGGGAAGGATCGTGGACTTATTAATCAAGAATTCACAGAGGTGGCAATTCCAGAAAATCTGATGGTTGGGACTACAGAAGGGCTGTTTGACTTCATTGCATCAGAACTTACTAAATTTGTTGGTAAAGAAGGTCCAGAATATCAACTCCCTCCTGGCAGGCAGAGAGAACTAGGTTTTACCTTCTCATTCCCTGTGCAGCAAACATCAATTAATTCTGGGTCCCTAATTAAGTGGACGAAAGGCTTCTCTATAGATGATACAGTTGGCCAAGATGTTGTGGCTGAATTGAGTAAAGCCATTGAAAAACAAGGGCTTGATATGCGCGTGTCCGCTCTGGTTAATGATACAGTTGGAACATTGGCTGGAGGCAGGTACAACAATAACGATGTTGTTGCAGCTGTGATTTTAGGTACTGGGACAAATGCAGCATATGTGGAACGTGCACAGGCTATTCCAAAATGGCATGGTCTTCTACCTAAATCTGGAGACATGGTTATCAACATGGAGTGGGGCAACTTCAGATCTGCACATCTTCCATTAACAGAATATGATCATTCATTGGACACTGAAAGTTTAAATCCGGGTGATCAGATTTTTGAGAAGATAATTTCGGGAATGTATTTGGGAGAAATTGTCCGCCGGGCTCTTTACAGGATTGCTGAAGAGGCTTCCATCTTTGATGAAACTGTTCCACCAAAATTAAAAGTTCCATTCATTTTGAGGACGCCTGATATGTCTGCAATGCATCATGATACTTCCTACGATCTTAAAGTTGTACGAGACATATTGAAGAATGTATTCGAGATATCAAATACTTCTCTTAAAGTAAGGAAACTTATTGTTGAGCTCTGCAATATTGTTGCTACACGTGGGGCTCGCCTTGCTGCTGCTGGAATCTTGGGGGTTCTGAAAAAGCTGGGAAGAGATGTGCACAGGGATGGGGAAAAACAGAAGACAGTGGTAGCTTTGGATGGTGGATTGTTTGAGCACTACACTGAATATAGTAAGTGCATGGAAAATACCCTAAGAGAATTGCTCGGAGAGGATGTTGGCGCATCTATTGTTATTGAGCACTCTAATGATGGTTCCGGAATTGGAGCTGCCCTTATTGCTGCTTCTCACTCACAATACCTCGGAGTTGATGAGTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230

Pfam Domains

Protein Families

Hexokinase_1 Hexokinase_2

Protein Analysis

497

Amino Acids

54.04

Weight (kDa)

5.95

Isoelectric Point (pI)

26.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hexokinase_1	PF00349	40 - 239	2.7e-73	Hexokinase
Hexokinase_2	PF03727	246 - 486	5.4e-83	Hexokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37840
fragaria_vesca	FvH4_1g18730 FvH4_6g32470 FvH4_6g32470 FvH4_6g32470 FvH4_6g32470
malus_domestica	MD02G1194700.v1.1 MD09G1202200.v1.1 MD15G1305100.v1.1 MD17G1183100.v1.1
prunus_persica	Prupe.3G057800_v2.0.a1 Prupe.6G212100_v2.0.a1
pyrus_communis	pycom07g16990 pycom09g11900 pycom09g11910 pycom15g26750 pycom17g19210
rosa_chinensis	RchiOBHm_Chr2g0109591 RchiOBHm_Chr2g0140711
rosa_laevigata	RLG00000017748 RLG00000019894
rosa_multiflora	Rmu_sc0000332.1_g000047 Rmu_sc0003342.1_g000014
rosa_roxburghii	Rroxscaffold_2G00134120
rosa_rugosa	Rorug02G0160900 Rorug02G0161000 Rorug02G0364300 Rorug02G0364400 Rorug02G0364500
rosa_samantha	Rh2AG213300 Rh2AG416000 Rh2BG223500 Rh2BG425600 Rh2CG215300 Rh2CG402200 Rh2DG218800 Rh2DG435500
rosa_wichuraiana	Rw2G034040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	702
AccBSI	CCGCTC	1 cut(s)	674
AccII	CGCG	1 cut(s)	666
AccIII	TCCGGA	1 cut(s)	1430
AciI	CCGC	9 cut(s)	14, 30, 38, 53, 56, 82, 328, 672, 979
AclWI	GGATC	1 cut(s)	357
AcoI	YGGCCR	4 cut(s)	91, 111, 171, 607
AcsI	RAATTY	2 cut(s)	371, 455
AcuI	CTGAAG	2 cut(s)	844, 1023
AcvI	CACGTG	1 cut(s)	1211
AcyI	GRCGYC	2 cut(s)	177, 1067
AfaI	GTAC	3 cut(s)	716, 754, 1119
AfiI	CCNNNNNNNGG	2 cut(s)	96, 824
AflIII	ACRYGT	1 cut(s)	1208
AgsI	TTSAA	3 cut(s)	335, 644, 1132
AjnI	CCWGG	1 cut(s)	493
AleI	CACNNNNGTG	1 cut(s)	380
Alw21I	GWGCWC	6 cut(s)	89, 787, 1191, 1275, 1332, 1416
Alw26I	GTCTC	4 cut(s)	157, 209, 822, 1116
Alw44I	GTGCAC	2 cut(s)	783, 1271
AlwI	GGATC	1 cut(s)	357
Aor13HI	TCCGGA	1 cut(s)	1430
AoxI	GGCC	4 cut(s)	91, 111, 171, 607
ApaLI	GTGCAC	2 cut(s)	783, 1271
ApeKI	GCWGC	9 cut(s)	35, 325, 538, 737, 767, 1226, 1229, 1442, 1454
ApoI	RAATTY	2 cut(s)	371, 455
AseI	ATTAAT	2 cut(s)	363, 552
Asp700I	GAANNNNTTC	3 cut(s)	584, 1029, 1139
AspLEI	GCGC	4 cut(s)	35, 78, 666, 1397
AspS9I	GGNCC	6 cut(s)	67, 99, 144, 298, 473, 561
AsuC2I	CCSGG	2 cut(s)	924, 983
AsuHPI	GGTGA	2 cut(s)	55, 938
AvaII	GGWCC	6 cut(s)	67, 99, 144, 298, 473, 561
BaeGI	GKGCMC	2 cut(s)	787, 1275
BalI	TGGCCA	1 cut(s)	609
BanII	GRGCYC	3 cut(s)	988, 1191, 1219
BbrPI	CACGTG	1 cut(s)	1211
BbsI	GAAGAC	2 cut(s)	803, 1301
Bbv12I	GWGCWC	6 cut(s)	89, 787, 1191, 1275, 1332, 1416
BbvI	GCAGC	9 cut(s)	22, 312, 550, 749, 779, 1213, 1216, 1429, 1441
BccI	CCATC	6 cut(s)	122, 397, 1022, 1274, 1307, 1418
BceAI	ACGGC	1 cut(s)	126
BcgI	CGANNNNNNTGC	2 cut(s)	716, 750
BciT130I	CCWGG	1 cut(s)	495
BclI	TGATCA	4 cut(s)	21, 237, 892, 928
BcnI	CCSGG	2 cut(s)	924, 983
BcoDI	GTCTC	4 cut(s)	157, 209, 822, 1116
BfaI	CTAG	1 cut(s)	512
BfmI	CTRYAG	2 cut(s)	414, 591
BfoI	RGCGCY	1 cut(s)	36
BfuAI	ACCTGC	1 cut(s)	702
BglII	AGATCT	1 cut(s)	863
Bme1390I	CCNGG	3 cut(s)	495, 924, 983
Bme18I	GGWCC	6 cut(s)	67, 99, 144, 298, 473, 561
BmgT120I	GGNCC	6 cut(s)	67, 99, 144, 298, 473, 561
BmiI	GGNNCC	5 cut(s)	145, 146, 562, 563, 1429
BmrFI	CCNGG	3 cut(s)	495, 924, 983
BmrI	ACTGGG	1 cut(s)	765
BmsI	GCATC	4 cut(s)	183, 449, 1096, 1406
BmuI	ACTGGG	1 cut(s)	765
BpiI	GAAGAC	2 cut(s)	803, 1301
BplI	GAGNNNNNCTC	2 cut(s)	1362, 1394
BpmI	CTGGAG	3 cut(s)	149, 726, 846
BpuEI	CTTGAG	1 cut(s)	215
BpuMI	CCSGG	2 cut(s)	924, 983
BsaAI	YACGTR	2 cut(s)	250, 1211
BsaBI	GATNNNNATC	1 cut(s)	239
BsaHI	GRCGYC	2 cut(s)	177, 1067
BsaI	GGTCTC	1 cut(s)	209
BsaJI	CCNNGG	4 cut(s)	48, 213, 301, 1474
BsaWI	WCCGGW	1 cut(s)	1430
BsaXI	ACNNNNNCTCC	6 cut(s)	819, 849, 960, 990, 1374, 1404
Bsc4I	CCNNNNNNNGG	2 cut(s)	96, 824
Bse118I	RCCGGY	1 cut(s)	200
Bse1I	ACTGG	1 cut(s)	760
Bse3DI	GCAATG	3 cut(s)	150, 435, 1089
Bse8I	GATNNNNATC	1 cut(s)	239
BseAI	TCCGGA	1 cut(s)	1430
BseBI	CCWGG	1 cut(s)	495
BseDI	CCNNGG	4 cut(s)	48, 213, 301, 1474
BseGI	GGATG	3 cut(s)	1285, 1318, 1393
BseJI	GATNNNNATC	1 cut(s)	239
BseLI	CCNNNNNNNGG	2 cut(s)	96, 824
BseMI	GCAATG	3 cut(s)	150, 435, 1089
BseMII	CTCAG	1 cut(s)	152
BseNI	ACTGG	1 cut(s)	760
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	2 cut(s)	787, 1275
BseXI	GCAGC	9 cut(s)	22, 312, 550, 749, 779, 1213, 1216, 1429, 1441
BseYI	CCCAGC	1 cut(s)	1258
BsgI	GTGCAG	2 cut(s)	557, 852
Bsh1236I	CGCG	1 cut(s)	666
Bsh1285I	CGRYCG	1 cut(s)	18
BshFI	GGCC	4 cut(s)	93, 113, 173, 609
BsiEI	CGRYCG	1 cut(s)	18
BsiHKAI	GWGCWC	6 cut(s)	89, 787, 1191, 1275, 1332, 1416
BsiSI	CCGG	5 cut(s)	90, 201, 923, 982, 1431
BslFI	GGGAC	4 cut(s)	157, 424, 547, 772
BslI	CCNNNNNNNGG	2 cut(s)	96, 824
BsmAI	GTCTC	4 cut(s)	157, 209, 822, 1116
BsmFI	GGGAC	4 cut(s)	157, 424, 547, 772
BsnI	GGCC	4 cut(s)	93, 113, 173, 609
Bso31I	GGTCTC	1 cut(s)	209
Bsp1286I	GDGCHC	8 cut(s)	89, 787, 988, 1191, 1219, 1275, 1332, 1416
Bsp13I	TCCGGA	1 cut(s)	1430
Bsp143I	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
BspACI	CCGC	9 cut(s)	14, 30, 38, 53, 56, 82, 328, 672, 979
BspANI	GGCC	4 cut(s)	93, 113, 173, 609
BspCNI	CTCAG	1 cut(s)	153
BspEI	TCCGGA	1 cut(s)	1430
BspFNI	CGCG	1 cut(s)	666
BspHI	TCATGA	1 cut(s)	1090
BspLI	GGNNCC	5 cut(s)	145, 146, 562, 563, 1429
BspMI	ACCTGC	1 cut(s)	702
BspPI	GGATC	1 cut(s)	357
BspTNI	GGTCTC	1 cut(s)	209
BsrBI	CCGCTC	1 cut(s)	674
BsrDI	GCAATG	3 cut(s)	150, 435, 1089
BsrFI	RCCGGY	1 cut(s)	200
BsrI	ACTGG	1 cut(s)	760
BssAI	RCCGGY	1 cut(s)	200
BssECI	CCNNGG	4 cut(s)	48, 213, 301, 1474
BssMI	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
BssNI	GRCGYC	2 cut(s)	177, 1067
BssT1I	CCWWGG	1 cut(s)	301
Bst2UI	CCWGG	1 cut(s)	495
Bst4CI	ACNGT	6 cut(s)	49, 187, 604, 691, 1030, 1300
Bst6I	CTCTTC	2 cut(s)	999, 1258
BstACI	GRCGYC	2 cut(s)	177, 1067
BstAPI	GCANNNNNTGC	1 cut(s)	84
BstBAI	YACGTR	2 cut(s)	250, 1211
BstC8I	GCNNGC	5 cut(s)	80, 198, 227, 500, 1219
BstDEI	CTNAG	2 cut(s)	161, 1367
BstDSI	CCRYGG	1 cut(s)	48
BstEII	GGTNACC	1 cut(s)	43
BstF5I	GGATG	3 cut(s)	1285, 1318, 1393
BstFNI	CGCG	1 cut(s)	666
BstH2I	RGCGCY	1 cut(s)	36
BstHHI	GCGC	4 cut(s)	35, 78, 666, 1397
BstKTI	GATC	7 cut(s)	24, 240, 352, 866, 895, 931, 1108
BstMAI	GTCTC	4 cut(s)	157, 209, 822, 1116
BstMBI	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
BstMCI	CGRYCG	1 cut(s)	18
BstMWI	GCNNNNNNNGC	6 cut(s)	84, 206, 661, 1007, 1223, 1451
BstNI	CCWGG	1 cut(s)	495
BstNSI	RCATGY	1 cut(s)	82
BstPI	GGTNACC	1 cut(s)	43
BstSCI	CCNGG	3 cut(s)	493, 922, 981
BstSFI	CTRYAG	2 cut(s)	414, 591
BstSLI	GKGCMC	2 cut(s)	787, 1275
BstUI	CGCG	1 cut(s)	666
BstV1I	GCAGC	9 cut(s)	22, 312, 550, 749, 779, 1213, 1216, 1429, 1441
BstV2I	GAAGAC	2 cut(s)	803, 1301
BstX2I	RGATCY	1 cut(s)	863
BstYI	RGATCY	1 cut(s)	863
BsuRI	GGCC	4 cut(s)	93, 113, 173, 609
BtgI	CCRYGG	1 cut(s)	48
BtsCI	GGATG	3 cut(s)	1285, 1318, 1393
BtsIMutI	CAGTG	3 cut(s)	906, 1305, 1335
BveI	ACCTGC	1 cut(s)	702
Cac8I	GCNNGC	5 cut(s)	80, 198, 227, 500, 1219
CciI	TCATGA	1 cut(s)	1090
CfoI	GCGC	4 cut(s)	35, 78, 666, 1397
Cfr10I	RCCGGY	1 cut(s)	200
Cfr13I	GGNCC	6 cut(s)	67, 99, 144, 298, 473, 561
CseI	GACGC	2 cut(s)	185, 1075
Csp6I	GTAC	3 cut(s)	715, 753, 1118
CviAII	CATG	7 cut(s)	79, 181, 806, 832, 844, 1091, 1354
CviQI	GTAC	3 cut(s)	715, 753, 1118
DdeI	CTNAG	2 cut(s)	161, 1367
DpnI	GATC	7 cut(s)	23, 239, 351, 865, 894, 930, 1107
DpnII	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
DraI	TTTAAA	1 cut(s)	918
EaeI	YGGCCR	4 cut(s)	91, 111, 171, 607
Eam1104I	CTCTTC	2 cut(s)	999, 1258
EarI	CTCTTC	2 cut(s)	999, 1258
EciI	GGCGGA	1 cut(s)	968
Ecl136II	GAGCTC	1 cut(s)	1189
Eco130I	CCWWGG	1 cut(s)	301
Eco24I	GRGCYC	3 cut(s)	988, 1191, 1219
Eco31I	GGTCTC	1 cut(s)	209
Eco32I	GATATC	1 cut(s)	1149
Eco47I	GGWCC	6 cut(s)	67, 99, 144, 298, 473, 561
Eco53kI	GAGCTC	1 cut(s)	1189
Eco57I	CTGAAG	2 cut(s)	844, 1023
Eco72I	CACGTG	1 cut(s)	1211
Eco91I	GGTNACC	1 cut(s)	43
EcoICRI	GAGCTC	1 cut(s)	1189
EcoO109I	RGGNCCY	2 cut(s)	144, 561
EcoO65I	GGTNACC	1 cut(s)	43
EcoRI	GAATTC	1 cut(s)	371
EcoRII	CCWGG	1 cut(s)	493
EcoRV	GATATC	1 cut(s)	1149
EcoT14I	CCWWGG	1 cut(s)	301
EcoT22I	ATGCAT	1 cut(s)	1089
EcoT38I	GRGCYC	3 cut(s)	988, 1191, 1219
ErhI	CCWWGG	1 cut(s)	301
FaeI	CATG	7 cut(s)	82, 184, 809, 835, 847, 1094, 1357
FaqI	GGGAC	4 cut(s)	157, 424, 547, 772
FatI	CATG	7 cut(s)	78, 180, 805, 831, 843, 1090, 1353
FauI	CCCGC	1 cut(s)	321
FauNDI	CATATG	1 cut(s)	772
FbaI	TGATCA	4 cut(s)	21, 237, 892, 928
FokI	GGATG	3 cut(s)	1292, 1325, 1400
FriOI	GRGCYC	3 cut(s)	988, 1191, 1219
FspBI	CTAG	1 cut(s)	512
GlaI	GCGC	4 cut(s)	34, 77, 665, 1396
GsaI	CCCAGC	1 cut(s)	1262
GsuI	CTGGAG	3 cut(s)	149, 726, 846
HaeII	RGCGCY	1 cut(s)	36
HaeIII	GGCC	4 cut(s)	93, 113, 173, 609
HapII	CCGG	5 cut(s)	90, 201, 923, 982, 1431
HgaI	GACGC	2 cut(s)	185, 1075
HhaI	GCGC	4 cut(s)	35, 78, 666, 1397
Hin1I	GRCGYC	2 cut(s)	177, 1067
Hin1II	CATG	7 cut(s)	82, 184, 809, 835, 847, 1094, 1357
Hin6I	GCGC	4 cut(s)	33, 76, 664, 1395
HinP1I	GCGC	4 cut(s)	33, 76, 664, 1395
HinfI	GANTC	2 cut(s)	1236, 1487
HpaII	CCGG	5 cut(s)	90, 201, 923, 982, 1431
HphI	GGTGA	2 cut(s)	55, 938
Hpy166II	GTNNAC	4 cut(s)	356, 576, 785, 1273
Hpy188I	TCNGA	8 cut(s)	214, 402, 445, 863, 933, 1251, 1382, 1478
Hpy8I	GTNNAC	4 cut(s)	356, 576, 785, 1273
Hpy99I	CGWCG	1 cut(s)	179
HpyAV	CCTTC	5 cut(s)	4, 340, 413, 464, 532
HpyCH4III	ACNGT	6 cut(s)	49, 187, 604, 691, 1030, 1300
HpyCH4IV	ACGT	3 cut(s)	249, 781, 1210
HpyF10VI	GCNNNNNNNGC	6 cut(s)	84, 206, 661, 1007, 1223, 1451
HpyF3I	CTNAG	2 cut(s)	161, 1367
HpySE526I	ACGT	3 cut(s)	249, 781, 1210
Hsp92I	GRCGYC	2 cut(s)	177, 1067
Hsp92II	CATG	7 cut(s)	82, 184, 809, 835, 847, 1094, 1357
HspAI	GCGC	4 cut(s)	33, 76, 664, 1395
KflI	GGGWCCC	2 cut(s)	144, 561
Kpn2I	TCCGGA	1 cut(s)	1430
Ksp22I	TGATCA	4 cut(s)	21, 237, 892, 928
Kzo9I	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
LmnI	GCTCC	3 cut(s)	92, 124, 1439
Lsp1109I	GCAGC	9 cut(s)	22, 312, 550, 749, 779, 1213, 1216, 1429, 1441
LweI	GCATC	4 cut(s)	183, 449, 1096, 1406
MaeI	CTAG	1 cut(s)	512
MaeII	ACGT	3 cut(s)	249, 781, 1210
MaeIII	GTNAC	1 cut(s)	43
MalI	GATC	7 cut(s)	23, 239, 351, 865, 894, 930, 1107
MbiI	CCGCTC	1 cut(s)	674
MboI	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
MboII	GAAGA	8 cut(s)	19, 803, 866, 955, 1016, 1144, 1275, 1306
MfeI	CAATTG	1 cut(s)	335
MflI	RGATCY	1 cut(s)	863
MhlI	GDGCHC	8 cut(s)	89, 787, 988, 1191, 1219, 1275, 1332, 1416
MlsI	TGGCCA	1 cut(s)	609
MluNI	TGGCCA	1 cut(s)	609
MmeI	TCCRAC	2 cut(s)	276, 673
Mox20I	TGGCCA	1 cut(s)	609
Mph1103I	ATGCAT	1 cut(s)	1089
MroI	TCCGGA	1 cut(s)	1430
MroXI	GAANNNNTTC	3 cut(s)	584, 1029, 1139
MscI	TGGCCA	1 cut(s)	609
MseI	TTAA	9 cut(s)	363, 552, 570, 681, 881, 917, 1043, 1110, 1164
MslI	CAYNNNNRTG	3 cut(s)	380, 1278, 1419
Msp20I	TGGCCA	1 cut(s)	609
MspA1I	CMGCKG	1 cut(s)	740
MspI	CCGG	5 cut(s)	90, 201, 923, 982, 1431
MspR9I	CCNGG	3 cut(s)	495, 924, 983
MunI	CAATTG	1 cut(s)	335
MvaI	CCWGG	1 cut(s)	495
MvnI	CGCG	1 cut(s)	666
MwoI	GCNNNNNNNGC	6 cut(s)	84, 206, 661, 1007, 1223, 1451
NciI	CCSGG	2 cut(s)	924, 983
NdeI	CATATG	1 cut(s)	772
NdeII	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
NlaIII	CATG	7 cut(s)	82, 184, 809, 835, 847, 1094, 1357
NlaIV	GGNNCC	5 cut(s)	145, 146, 562, 563, 1429
NmuCI	GTSAC	1 cut(s)	43
NsiI	ATGCAT	1 cut(s)	1089
NspI	RCATGY	1 cut(s)	82
OliI	CACNNNNGTG	1 cut(s)	380
PaeI	GCATGC	1 cut(s)	82
PagI	TCATGA	1 cut(s)	1090
PdmI	GAANNNNTTC	3 cut(s)	584, 1029, 1139
PfeI	GAWTC	1 cut(s)	1236
PmaCI	CACGTG	1 cut(s)	1211
PmlI	CACGTG	1 cut(s)	1211
Ppu21I	YACGTR	2 cut(s)	250, 1211
PpuMI	RGGWCCY	2 cut(s)	144, 561
PshBI	ATTAAT	2 cut(s)	363, 552
Psp124BI	GAGCTC	1 cut(s)	1191
Psp5II	RGGWCCY	2 cut(s)	144, 561
Psp6I	CCWGG	1 cut(s)	493
PspCI	CACGTG	1 cut(s)	1211
PspEI	GGTNACC	1 cut(s)	43
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	1 cut(s)	493
PspN4I	GGNNCC	5 cut(s)	145, 146, 562, 563, 1429
PspPI	GGNCC	6 cut(s)	67, 99, 144, 298, 473, 561
PspPPI	RGGWCCY	2 cut(s)	144, 561
PsuI	RGATCY	1 cut(s)	863
PvuII	CAGCTG	1 cut(s)	740
RsaI	GTAC	3 cut(s)	716, 754, 1119
RsaNI	GTAC	3 cut(s)	715, 753, 1118
RseI	CAYNNNNRTG	3 cut(s)	380, 1278, 1419
SacI	GAGCTC	1 cut(s)	1191
SaqAI	TTAA	9 cut(s)	363, 552, 570, 681, 881, 917, 1043, 1110, 1164
Sau3AI	GATC	7 cut(s)	21, 237, 349, 863, 892, 928, 1105
Sau96I	GGNCC	6 cut(s)	67, 99, 144, 298, 473, 561
ScrFI	CCNGG	3 cut(s)	495, 924, 983
SduI	GDGCHC	8 cut(s)	89, 787, 988, 1191, 1219, 1275, 1332, 1416
SfaNI	GCATC	4 cut(s)	183, 449, 1096, 1406
SfcI	CTRYAG	2 cut(s)	414, 591
SinI	GGWCC	6 cut(s)	67, 99, 144, 298, 473, 561
SmiMI	CAYNNNNRTG	3 cut(s)	380, 1278, 1419
SmlI	CTYRAG	1 cut(s)	230
SmoI	CTYRAG	1 cut(s)	230
SphI	GCATGC	1 cut(s)	82
SsiI	CCGC	9 cut(s)	14, 30, 38, 53, 56, 82, 328, 672, 979
SspI	AATATT	1 cut(s)	1198
SspMI	CTAG	1 cut(s)	512
SstI	GAGCTC	1 cut(s)	1191
StyD4I	CCNGG	3 cut(s)	493, 922, 981
StyI	CCWWGG	1 cut(s)	301
TaaI	ACNGT	6 cut(s)	49, 187, 604, 691, 1030, 1300
TaiI	ACGT	3 cut(s)	252, 784, 1213
TaqI	TCGA	2 cut(s)	189, 1143
TauI	GCSGC	3 cut(s)	33, 56, 59
TfiI	GAWTC	1 cut(s)	1236
Tru1I	TTAA	9 cut(s)	363, 552, 570, 681, 881, 917, 1043, 1110, 1164
Tru9I	TTAA	9 cut(s)	363, 552, 570, 681, 881, 917, 1043, 1110, 1164
TscAI	CASTG	3 cut(s)	913, 1305, 1342
TseFI	GTSAC	1 cut(s)	43
TseI	GCWGC	9 cut(s)	35, 325, 538, 737, 767, 1226, 1229, 1442, 1454
Tsp45I	GTSAC	1 cut(s)	43
TspDTI	ATGAA	4 cut(s)	424, 888, 1038, 1045
TspGWI	ACGGA	2 cut(s)	59, 308
TspRI	CASTG	3 cut(s)	913, 1305, 1342
VneI	GTGCAC	2 cut(s)	783, 1271
VpaK11BI	GGWCC	6 cut(s)	67, 99, 144, 298, 473, 561
VspI	ATTAAT	2 cut(s)	363, 552
XapI	RAATTY	2 cut(s)	371, 455
XceI	RCATGY	1 cut(s)	82
XmnI	GAANNNNTTC	3 cut(s)	584, 1029, 1139
XspI	CTAG	1 cut(s)	512
Zsp2I	ATGCAT	1 cut(s)	1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.