MD17G1223900.v1.1

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014769
Physical Location & Seq
Reverse (-)
27250571 .. 27252636
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1223900.v1.1.491

Sequence Viewer

Length: 531 bp
ATGTCCAAGTCCTCCTCCAACACCAAATTCCACGACCACATCTTGCTTCTCGTTTTCATCATCTCGGGGCTTGCAGTGCTCACTCCAATCCTTGCCACTGACCACATTGTTGGAGCCAACCGTGGCTGGAATCCTGGCCAAAACTACACCCTCTGGGCCAACAACCATACTTTCTATGTTGGGGACCTTATCTCGTTTAGGTACCAAAAGAACCAGTACAATGTGTTTGAAGTGAACCAAACAGGGTATGACAACTGCACCTTGGATGGTGCAGTTGGGAACTGGAGCAGTGGCAAAGACTTCATCCCCCTCAATCAGGCGAAGAGGTATTACTTCATTTGTGGCAATGGCCAGTGCTACAGTGGCATGAAGGTCTCTGTCCTTGTCCATCCTCTGCCTTCCCCCCCAACGGCCCCCGTCAGTGCTCATAACTCTTCATCAGATGCTGCTGTCCTGGTGGTTCCTCAACAGGGATTTCGGGCCTTGGTGGTTTCCTTGGCAATGCTGTGCTTGGGATTTGGATGGGTGTAG

Protein Analysis

177

Amino Acids

19.27

Weight (kDa)

8.36

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 123 1.7e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014769)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28520 FvH4_6g28520
malus_domestica MD09G1225200.v1.1 MD17G1223900.v1.1
prunus_persica Prupe.3G099600_v2.0.a1
pyrus_communis pycom17g22780
rosa_chinensis RchiOBHm_Chr2g0132811
rosa_laevigata RLG00000019284
rosa_multiflora Rmu_co8274343.1_g000001 Rmu_sc0004972.1_g000006 Rmu_sc0007091.1_g000001
rosa_rugosa Rorug02G0307800
rosa_samantha Rh2AG360200 Rh2BG366100 Rh2CG343500 Rh2DG383100
rosa_wichuraiana Rw2G029280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AcoI YGGCCR 2 cut(s) 136, 349
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 2 cut(s) 203, 218
AfiI CCNNNNNNNGG 3 cut(s) 316, 409, 470
AgsI TTSAA 1 cut(s) 230
AjnI CCWGG 2 cut(s) 133, 453
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
Alw21I GWGCWC 2 cut(s) 81, 427
Alw26I GTCTC 1 cut(s) 379
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 64
AoxI GGCC 5 cut(s) 136, 156, 349, 411, 480
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 26
Asp718I GGTACC 1 cut(s) 201
AspS9I GGNCC 4 cut(s) 156, 184, 412, 480
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 1 cut(s) 184
BalI TGGCCA 2 cut(s) 138, 351
BanI GGYRCC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
Bbv12I GWGCWC 2 cut(s) 81, 427
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 260, 396, 516
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 2 cut(s) 135, 455
BcoDI GTCTC 1 cut(s) 379
BfmI CTRYAG 1 cut(s) 358
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 135, 455
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 64
BmgT120I GGNCC 4 cut(s) 156, 184, 412, 480
BmiI GGNNCC 5 cut(s) 115, 185, 203, 414, 462
BmrFI CCNGG 2 cut(s) 135, 455
BmsI GCATC 1 cut(s) 433
BpmI CTGGAG 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 4 cut(s) 121, 261, 483, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 409, 470
Bse1I ACTGG 3 cut(s) 214, 287, 352
Bse3DI GCAATG 2 cut(s) 352, 507
BseBI CCWGG 2 cut(s) 135, 455
BseDI CCNNGG 4 cut(s) 121, 261, 483, 495
BseGI GGATG 4 cut(s) 271, 303, 388, 527
BseLI CCNNNNNNNGG 3 cut(s) 316, 409, 470
BseMI GCAATG 2 cut(s) 352, 507
BseNI ACTGG 3 cut(s) 214, 287, 352
BseRI GAGGAG 1 cut(s) 4
BseXI GCAGC 1 cut(s) 433
BsgI GTGCAG 2 cut(s) 241, 291
BshFI GGCC 5 cut(s) 138, 158, 351, 413, 482
BshNI GGYRCC 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 81, 427
BsiHKCI CYCGRG 1 cut(s) 64
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 3 cut(s) 316, 409, 470
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 5 cut(s) 138, 158, 351, 413, 482
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 81, 427
BspANI GGCC 5 cut(s) 138, 158, 351, 413, 482
BspLI GGNNCC 5 cut(s) 115, 185, 203, 414, 462
BspT107I GGYRCC 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 352, 507
BsrI ACTGG 3 cut(s) 214, 287, 352
BssECI CCNNGG 4 cut(s) 121, 261, 483, 495
BssT1I CCWWGG 3 cut(s) 261, 483, 495
Bst2UI CCWGG 2 cut(s) 135, 455
Bst4CI ACNGT 2 cut(s) 122, 362
Bst6I CTCTTC 2 cut(s) 317, 439
BstC8I GCNNGC 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 121
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 4 cut(s) 271, 303, 388, 527
BstMAI GTCTC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 76, 363
BstNI CCWGG 2 cut(s) 135, 455
BstSCI CCNGG 2 cut(s) 133, 453
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 110
BsuRI GGCC 5 cut(s) 138, 158, 351, 413, 482
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 4 cut(s) 271, 303, 388, 527
BtsI GCAGTG 2 cut(s) 81, 295
BtsIMutI CAGTG 6 cut(s) 81, 96, 295, 359, 367, 427
Cac8I GCNNGC 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 446
Cfr13I GGNCC 4 cut(s) 156, 184, 412, 480
Csp6I GTAC 2 cut(s) 202, 217
CviAII CATG 1 cut(s) 367
CviJI RGCY 8 cut(s) 70, 116, 126, 138, 158, 351, 413, 482
CviKI_1 RGCY 8 cut(s) 70, 116, 126, 138, 158, 351, 413, 482
CviQI GTAC 2 cut(s) 202, 217
EaeI YGGCCR 2 cut(s) 136, 349
Eam1104I CTCTTC 2 cut(s) 317, 439
EarI CTCTTC 2 cut(s) 317, 439
Eco130I CCWWGG 3 cut(s) 261, 483, 495
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 133, 453
EcoT14I CCWWGG 3 cut(s) 261, 483, 495
ErhI CCWWGG 3 cut(s) 261, 483, 495
FaeI CATG 1 cut(s) 370
FaiI YATR 5 cut(s) 168, 177, 249, 368, 429
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 3 cut(s) 278, 290, 375
Fsp4HI GCNGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 304
HaeIII GGCC 5 cut(s) 138, 158, 351, 413, 482
Hin1II CATG 1 cut(s) 370
HinfI GANTC 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 1 cut(s) 442
Hpy8I GTNNAC 1 cut(s) 235
HpyAV CCTTC 2 cut(s) 364, 408
HpyCH4III ACNGT 2 cut(s) 122, 362
HpyCH4V TGCA 3 cut(s) 74, 258, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 363
Hsp92II CATG 1 cut(s) 370
KpnI GGTACC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 113, 285
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 433
MboII GAAGA 2 cut(s) 334, 426
MhlI GDGCHC 2 cut(s) 81, 427
MlsI TGGCCA 2 cut(s) 138, 351
MluCI AATT 1 cut(s) 26
MluNI TGGCCA 2 cut(s) 138, 351
MmeI TCCRAC 2 cut(s) 42, 91
MnlI CCTC 7 cut(s) 22, 25, 161, 318, 320, 402, 474
Mox20I TGGCCA 2 cut(s) 138, 351
MscI TGGCCA 2 cut(s) 138, 351
Msp20I TGGCCA 2 cut(s) 138, 351
MspR9I CCNGG 2 cut(s) 135, 455
MvaI CCWGG 2 cut(s) 135, 455
MwoI GCNNNNNNNGC 2 cut(s) 76, 363
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 5 cut(s) 115, 185, 203, 414, 462
PfeI GAWTC 1 cut(s) 130
PkrI GCNGC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 133, 453
PspGI CCWGG 2 cut(s) 133, 453
PspN4I GGNNCC 5 cut(s) 115, 185, 203, 414, 462
PspPI GGNCC 4 cut(s) 156, 184, 412, 480
PspPPI RGGWCCY 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 203, 218
RsaNI GTAC 2 cut(s) 202, 217
SatI GCNGC 1 cut(s) 447
Sau96I GGNCC 4 cut(s) 156, 184, 412, 480
ScrFI CCNGG 2 cut(s) 135, 455
SduI GDGCHC 2 cut(s) 81, 427
SetI ASST 5 cut(s) 189, 203, 263, 329, 375
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 184
Sse9I AATT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 133, 453
StyI CCWWGG 3 cut(s) 261, 483, 495
TaaI ACNGT 2 cut(s) 122, 362
TasI AATT 1 cut(s) 26
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 130
TscAI CASTG 6 cut(s) 81, 103, 295, 359, 367, 427
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 5 cut(s) 46, 292, 325, 383, 426
TspRI CASTG 6 cut(s) 81, 103, 295, 359, 367, 427
VpaK11BI GGWCC 1 cut(s) 184
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.