Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011873

Physical Location & Seq

Genomic Coordinates Forward (+)

28293879 .. 28298759

Gene Structure 4881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1234100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGGATCGCGTGACGTGGCGAACTCGAGTCCTCTCGAATCATCTCAACCAACCCTCATGGAGCTCGAACCCTCTTTCCCCCAACCCCTGCCTCGGCTTTTCTCCTCCCGAGCTCTCGGAGCCCTTCGAATTCGACACCAAGGAGATGCGCAAGCTCCTCGACGCGCACAATCTTGAGGACCGGGACTGGCTCTTCGGCCTCATGAAGCAGAGCAAGCTCTTTAATCCGAGAGAGGCTGGGGGCCGGGTGTTTATCGCGCCGGACTACAACCAAAGCATGGAGCAGCAGCGGCAGATGACCATGAAGCGAATTGTCTACTTGCTGGATCGCGGGGTTTTCAAAGGCTGGCTTACGGATAAAGGGGTCGACGCCGAGTTGCGAAAGTTTGCGTTTTTCGAGGTTATCGGGATTTTTGATCATTCCCTTGCTGTCAAGCTTGGTGTCCATTTCTTTTTGTGGGGCGGTGCCATTCAGTTTTTCGGCACAAAGCGCCATCACGACAAGTGGCTGAGTGACACTGAGAACTATGCAATCAAGGGTTGCTTTGCAATGTCTGAGTTGGGCCATGGCAGTAATGTTCGAGGGATTGAAACAGTCACCACTTATGATTCGAGTACTGGAGAATTTGTTATTAATACTCCTTGCGAATCAGCTCAGAAGTATTGGATTGGAGGAGCAGCTAATCATGCCACACACACAATAGTCTTTTCACAACTCAGTATATCCGGGAACAACCAAGGCGTCCATGCTCTTATAGCCCAGATCAGGGATGAAAATGGTAATGTCTGTCCAGGAGTTCGTATTGCTGATTGTGGTCACAAAATTGGCTTAAATGGTGTTGATAATGGTCGAATATGGTTTGACAACATTCGAATTCCGAGGGAAAATTTGTTAAATTCAGTAGCTGATGTTTCTCCAGATGGGAAATATCTGAGTGCAATAAAAGACCCAGATCAGAGATTTGCAGCATTCATGGCTCCATTGACATCTGGTCGTGTAACTATAGCAGTCAGTTCCATTTACTCATCACAGATTAGTTTAGCAATAGCCATAAGGTACTCATTGACAAGGCGGGCCTTTTCTGTTACGCCAAATGGGCCTGAAGTCCTATTGCTTGACTACCCCAGTCATCAACAGCGGCTTTTGCCTCTTCTTGCAAAGACATATGCTATGACTTTTGCTTCAAATTACTTAAAGATGATTTATGTTAAGAGGACGCCTGAGTCGAGCAAAACCATCCATGTTGTTTCTAGTGCATTTAAGGCTATACATACCTGGCATAACATGCGGACTCTTCAGGAATGCCGCGAAGCCTGTGGAGGGCAAGGTATCAAGACTGAAAACCGTGTTGGTCATTTTAAAGGTGAATTTGATGTGCAGTCCACTTTTGAGGGGGACAACAATGTTTTGATGCAACAGGTCAGCAAAGCACTGCTTGCCGAGTATATAGCAACTCAGAAGAGAGGCAAACCATTTAAAGCTTTGGGACTAGAGCATATGAATAAACCTTGCCCTGTCATTCCATCTCAACTTACAAGTTCTACCCTCCAGTGTAGCCAATTCCAGATGGATGCTTTCTGCTTAAGAGAGCGAGATCTGTTGAATAGATTTGCTATGGAAGTGTCACAACGTCAAAAACAGGGAGAAAGCAAAGAGTACTCGATCATTCTTAGTTATCAGCTTGCAGGGGACCTGGGCAGAGCTTTTGCAGATCGATCAATATTGCAAACCTTCATAGATGCTGAGGCGGCACTACCTGCTGGTTCATTAAAGAATGTCTTAGCTCTGTTGAGGTCTATGTACGCATTGATTTGCTTGGAAGAAGATGCGGCATTCCTCCGATATGGGTACTTATCGACCAATAATGTCGCTGCTGTGAGGAAAGAAGTAAGCAGACTGTGTGGTGAATTGCGGCCGCATGCACTGGCCTTGGTGAGTTCCTTCGGCATCCCCGATGCTTTTCTGAGTCCCATAGCTTTTAACTGGATCGATTCAAATTCGTGGTCTTCAGTTGAGCAGCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0046395 GO:0048037 GO:0050660 GO:0050662 GO:0051791 GO:0055114 GO:0071704 GO:0072329 GO:0097159 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_1 Acyl-CoA_dh_M

Protein Analysis

675

Amino Acids

75.52

Weight (kDa)

8.46

Isoelectric Point (pI)

36.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_M	PF02770	181 - 289	1.4e-11	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	321 - 479	1.2e-09	Acyl-CoA dehydrogenase, C-terminal domain
ACOX_C_alpha1	PF22924	328 - 479	1.1e-39	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	522 - 655	6.5e-19	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06290 AT1G06310 AT3G06690
fragaria_vesca	FvH4_6g26900 FvH4_6g26900 FvH4_6g26900
malus_domestica	MD17G1234100.v1.1
prunus_persica	Prupe.3G127000_v2.0.a1
pyrus_communis	pycom17g23740
rosa_chinensis	RchiOBHm_Chr2g0128641
rosa_laevigata	RLG00000019014
rosa_multiflora	Rmu_sc0000837.1_g000086 Rmu_sc0003465.1_g000047
rosa_roxburghii	Rroxscaffold_2G00115420 Rroxscaffold_2G00115900
rosa_rugosa	Rorug02G0279600
rosa_samantha	Rh2DG357400
rosa_wichuraiana	Rw2G026890 Rw2G027150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1222
Acc16I	TGCGCA	1 cut(s)	149
Acc36I	ACCTGC	1 cut(s)	1765
AccB1I	GGYRCC	1 cut(s)	464
AccB7I	CCANNNNNTGG	1 cut(s)	277
AccI	GTMKAC	2 cut(s)	315, 366
AccII	CGCG	5 cut(s)	9, 164, 257, 330, 1308
AclWI	GGATC	3 cut(s)	12, 333, 1994
AcoI	YGGCCR	1 cut(s)	1913
AcsI	RAATTY	7 cut(s)	128, 623, 873, 886, 895, 1367, 1996
AcuI	CTGAAG	3 cut(s)	1122, 1280, 1992
AcyI	GRCGYC	3 cut(s)	369, 741, 1217
AfaI	GTAC	5 cut(s)	616, 1058, 1658, 1802, 1850
AfiI	CCNNNNNNNGG	5 cut(s)	92, 277, 765, 766, 1320
AflII	CTTAAG	1 cut(s)	1582
AgsI	TTSAA	5 cut(s)	340, 590, 1185, 1603, 1995
AhdI	GACNNNNNGTC	1 cut(s)	990
AjiI	CACGTC	1 cut(s)	15
AjnI	CCWGG	3 cut(s)	790, 1274, 1692
AjuI	GAANNNNNNNTTGG	2 cut(s)	1332, 1364
AloI	GAACNNNNNNTCC	2 cut(s)	59, 91
Alw21I	GWGCWC	2 cut(s)	65, 114
AlwI	GGATC	3 cut(s)	12, 333, 1994
AlwNI	CAGNNNCTG	1 cut(s)	905
Ama87I	CYCGRG	2 cut(s)	24, 107
AoxI	GGCC	7 cut(s)	196, 241, 562, 1074, 1097, 1913, 1926
ApeKI	GCWGC	6 cut(s)	283, 286, 677, 965, 1871, 2017
ApoI	RAATTY	7 cut(s)	128, 623, 873, 886, 895, 1367, 1996
ArsI	GACNNNNNNTTYG	4 cut(s)	176, 208, 1988, 2020
AseI	ATTAAT	1 cut(s)	633
AspLEI	GCGC	4 cut(s)	150, 166, 259, 492
AspS9I	GGNCC	6 cut(s)	178, 241, 562, 1074, 1097, 1690
AsuC2I	CCSGG	3 cut(s)	182, 245, 727
AsuHPI	GGTGA	4 cut(s)	589, 1376, 1916, 1945
AsuII	TTCGAA	2 cut(s)	126, 871
AvaI	CYCGRG	2 cut(s)	24, 107
AvaII	GGWCC	2 cut(s)	178, 1690
BanI	GGYRCC	1 cut(s)	464
BanII	GRGCYC	3 cut(s)	65, 114, 123
BbsI	GAAGAC	1 cut(s)	1998
Bbv12I	GWGCWC	2 cut(s)	65, 114
BbvCI	CCTCAGC	1 cut(s)	1743
BbvI	GCAGC	5 cut(s)	295, 298, 689, 977, 1858
BccI	CCATC	5 cut(s)	501, 914, 1244, 1531, 1561
BcgI	CGANNNNNNTGC	2 cut(s)	139, 173
BciT130I	CCWGG	3 cut(s)	792, 1276, 1694
BclI	TGATCA	1 cut(s)	415
BcnI	CCSGG	3 cut(s)	182, 245, 727
BfaI	CTAG	3 cut(s)	1251, 1490, 2023
BfmI	CTRYAG	1 cut(s)	1002
BfoI	RGCGCY	1 cut(s)	493
BfrI	CTTAAG	1 cut(s)	1582
BfuAI	ACCTGC	1 cut(s)	1765
BglI	GCCNNNNNGGC	1 cut(s)	1096
BglII	AGATCT	1 cut(s)	1594
BmcAI	AGTACT	2 cut(s)	616, 1658
Bme1390I	CCNGG	6 cut(s)	182, 245, 727, 792, 1276, 1694
Bme18I	GGWCC	2 cut(s)	178, 1690
BmeRI	GACNNNNNGTC	1 cut(s)	990
BmeT110I	CYCGRG	2 cut(s)	24, 107
BmgBI	CACGTC	1 cut(s)	15
BmgT120I	GGNCC	6 cut(s)	178, 241, 562, 1074, 1097, 1690
BmiI	GGNNCC	5 cut(s)	120, 242, 466, 978, 1691
BmrFI	CCNGG	6 cut(s)	182, 245, 727, 792, 1276, 1694
BmrI	ACTGGG	1 cut(s)	1119
BmsI	GCATC	7 cut(s)	135, 1401, 1561, 1729, 1816, 1945, 1956
BmuI	ACTGGG	1 cut(s)	1119
BpiI	GAAGAC	1 cut(s)	1998
BpmI	CTGGAG	3 cut(s)	639, 900, 1532
Bpu10I	CCTNAGC	1 cut(s)	1743
Bpu14I	TTCGAA	2 cut(s)	126, 871
BpuEI	CTTGAG	1 cut(s)	194
BpuMI	CCSGG	3 cut(s)	182, 245, 727
Bsa29I	ATCGAT	2 cut(s)	1714, 1989
BsaHI	GRCGYC	3 cut(s)	369, 741, 1217
BsaJI	CCNNGG	7 cut(s)	91, 138, 565, 736, 878, 1693, 1929
BsaXI	ACNNNNNCTCC	2 cut(s)	612, 642
Bsc4I	CCNNNNNNNGG	5 cut(s)	92, 277, 765, 766, 1320
Bse1I	ACTGG	6 cut(s)	191, 622, 1125, 1549, 1929, 1988
Bse3DI	GCAATG	1 cut(s)	555
BseBI	CCWGG	3 cut(s)	792, 1276, 1694
BseCI	ATCGAT	2 cut(s)	1714, 1989
BseDI	CCNNGG	7 cut(s)	91, 138, 565, 736, 878, 1693, 1929
BseGI	GGATG	4 cut(s)	775, 1236, 1576, 1947
BseLI	CCNNNNNNNGG	5 cut(s)	92, 277, 765, 766, 1320
BseMI	GCAATG	1 cut(s)	555
BseNI	ACTGG	6 cut(s)	191, 622, 1125, 1549, 1929, 1988
BseRI	GAGGAG	3 cut(s)	93, 146, 687
BseX3I	CGGCCG	1 cut(s)	1913
BseXI	GCAGC	5 cut(s)	295, 298, 689, 977, 1858
BseYI	CCCAGC	1 cut(s)	236
BsgI	GTGCAG	1 cut(s)	1397
Bsh1236I	CGCG	5 cut(s)	9, 164, 257, 330, 1308
Bsh1285I	CGRYCG	1 cut(s)	1916
BshFI	GGCC	7 cut(s)	198, 243, 564, 1076, 1099, 1915, 1928
BshNI	GGYRCC	1 cut(s)	464
BshVI	ATCGAT	2 cut(s)	1714, 1989
BsiEI	CGRYCG	1 cut(s)	1916
BsiHKAI	GWGCWC	2 cut(s)	65, 114
BsiHKCI	CYCGRG	2 cut(s)	24, 107
BsiSI	CCGG	4 cut(s)	181, 244, 260, 726
BslFI	GGGAC	5 cut(s)	197, 1409, 1500, 1703, 1953
BslI	CCNNNNNNNGG	5 cut(s)	92, 277, 765, 766, 1320
BsmFI	GGGAC	5 cut(s)	197, 1409, 1500, 1703, 1953
BsmI	GAATGC	3 cut(s)	968, 1307, 1832
BsnI	GGCC	7 cut(s)	198, 243, 564, 1076, 1099, 1915, 1928
BsoBI	CYCGRG	2 cut(s)	24, 107
Bsp119I	TTCGAA	2 cut(s)	126, 871
Bsp1286I	GDGCHC	3 cut(s)	65, 114, 123
Bsp19I	CCATGG	1 cut(s)	565
BspANI	GGCC	7 cut(s)	198, 243, 564, 1076, 1099, 1915, 1928
BspDI	ATCGAT	2 cut(s)	1714, 1989
BspFNI	CGCG	5 cut(s)	9, 164, 257, 330, 1308
BspHI	TCATGA	1 cut(s)	201
BspLI	GGNNCC	5 cut(s)	120, 242, 466, 978, 1691
BspMI	ACCTGC	1 cut(s)	1765
BspPI	GGATC	3 cut(s)	12, 333, 1994
BspQI	GCTCTTC	1 cut(s)	197
BspT104I	TTCGAA	2 cut(s)	126, 871
BspT107I	GGYRCC	1 cut(s)	464
BspTI	CTTAAG	1 cut(s)	1582
BsrDI	GCAATG	1 cut(s)	555
BsrI	ACTGG	6 cut(s)	191, 622, 1125, 1549, 1929, 1988
BssECI	CCNNGG	7 cut(s)	91, 138, 565, 736, 878, 1693, 1929
BssNI	GRCGYC	3 cut(s)	369, 741, 1217
BssT1I	CCWWGG	4 cut(s)	138, 565, 736, 1929
Bst2UI	CCWGG	3 cut(s)	792, 1276, 1694
Bst4CI	ACNGT	3 cut(s)	595, 1346, 1899
Bst6I	CTCTTC	4 cut(s)	197, 1155, 1299, 1454
BstACI	GRCGYC	3 cut(s)	369, 741, 1217
BstAFI	CTTAAG	1 cut(s)	1582
BstAPI	GCANNNNNTGC	3 cut(s)	1285, 1436, 1757
BstBI	TTCGAA	2 cut(s)	126, 871
BstC8I	GCNNGC	7 cut(s)	152, 215, 347, 1074, 1437, 1683, 1920
BstDSI	CCRYGG	1 cut(s)	565
BstF5I	GGATG	4 cut(s)	775, 1236, 1576, 1947
BstFNI	CGCG	5 cut(s)	9, 164, 257, 330, 1308
BstH2I	RGCGCY	1 cut(s)	493
BstHHI	GCGC	4 cut(s)	150, 166, 259, 492
BstMCI	CGRYCG	1 cut(s)	1916
BstNI	CCWGG	3 cut(s)	792, 1276, 1694
BstNSI	RCATGY	2 cut(s)	1288, 1922
BstSCI	CCNGG	6 cut(s)	180, 243, 725, 790, 1274, 1692
BstSFI	CTRYAG	1 cut(s)	1002
BstUI	CGCG	5 cut(s)	9, 164, 257, 330, 1308
BstV1I	GCAGC	5 cut(s)	295, 298, 689, 977, 1858
BstV2I	GAAGAC	1 cut(s)	1998
BstX2I	RGATCY	1 cut(s)	1594
BstYI	RGATCY	1 cut(s)	1594
BstZI	CGGCCG	1 cut(s)	1913
Bsu15I	ATCGAT	2 cut(s)	1714, 1989
BsuRI	GGCC	7 cut(s)	198, 243, 564, 1076, 1099, 1915, 1928
BsuTUI	ATCGAT	2 cut(s)	1714, 1989
BtgI	CCRYGG	1 cut(s)	565
BtrI	CACGTC	1 cut(s)	15
BtsCI	GGATG	4 cut(s)	775, 1236, 1576, 1947
BtsI	GCAGTG	1 cut(s)	1430
BtsIMutI	CAGTG	4 cut(s)	516, 1430, 1556, 1922
BveI	ACCTGC	1 cut(s)	1765
Cac8I	GCNNGC	7 cut(s)	152, 215, 347, 1074, 1437, 1683, 1920
CaiI	CAGNNNCTG	1 cut(s)	905
CciI	TCATGA	1 cut(s)	201
CciNI	GCGGCCGC	1 cut(s)	1913
CfoI	GCGC	4 cut(s)	150, 166, 259, 492
Cfr13I	GGNCC	6 cut(s)	178, 241, 562, 1074, 1097, 1690
ClaI	ATCGAT	2 cut(s)	1714, 1989
CseI	GACGC	4 cut(s)	170, 377, 730, 1225
Csp6I	GTAC	5 cut(s)	615, 1057, 1657, 1801, 1849
CviQI	GTAC	5 cut(s)	615, 1057, 1657, 1801, 1849
DraI	TTTAAA	2 cut(s)	1360, 1477
DrdI	GACNNNNNNGTC	1 cut(s)	1222
DriI	GACNNNNNGTC	1 cut(s)	990
DseDI	GACNNNNNNGTC	1 cut(s)	1222
EaeI	YGGCCR	1 cut(s)	1913
EagI	CGGCCG	1 cut(s)	1913
Eam1104I	CTCTTC	4 cut(s)	197, 1155, 1299, 1454
Eam1105I	GACNNNNNGTC	1 cut(s)	990
EarI	CTCTTC	4 cut(s)	197, 1155, 1299, 1454
Ecl136II	GAGCTC	2 cut(s)	63, 112
EclXI	CGGCCG	1 cut(s)	1913
Eco130I	CCWWGG	4 cut(s)	138, 565, 736, 1929
Eco24I	GRGCYC	3 cut(s)	65, 114, 123
Eco47I	GGWCC	2 cut(s)	178, 1690
Eco52I	CGGCCG	1 cut(s)	1913
Eco53kI	GAGCTC	2 cut(s)	63, 112
Eco57I	CTGAAG	3 cut(s)	1122, 1280, 1992
Eco88I	CYCGRG	2 cut(s)	24, 107
EcoICRI	GAGCTC	2 cut(s)	63, 112
EcoO109I	RGGNCCY	1 cut(s)	1690
EcoRI	GAATTC	2 cut(s)	128, 873
EcoRII	CCWGG	3 cut(s)	790, 1274, 1692
EcoT14I	CCWWGG	4 cut(s)	138, 565, 736, 1929
EcoT38I	GRGCYC	3 cut(s)	65, 114, 123
ErhI	CCWWGG	4 cut(s)	138, 565, 736, 1929
FalI	AAGNNNNNCTT	8 cut(s)	333, 365, 527, 559, 1419, 1451, 1763, 1795
FaqI	GGGAC	5 cut(s)	197, 1409, 1500, 1703, 1953
FauI	CCCGC	2 cut(s)	323, 1065
FauNDI	CATATG	2 cut(s)	1165, 1497
FbaI	TGATCA	1 cut(s)	415
FblI	GTMKAC	2 cut(s)	315, 366
FokI	GGATG	4 cut(s)	782, 1223, 1583, 1934
FriOI	GRGCYC	3 cut(s)	65, 114, 123
FspBI	CTAG	3 cut(s)	1251, 1490, 2023
FspI	TGCGCA	1 cut(s)	149
GlaI	GCGC	4 cut(s)	149, 165, 258, 491
GsaI	CCCAGC	1 cut(s)	240
GsuI	CTGGAG	3 cut(s)	639, 900, 1532
HaeII	RGCGCY	1 cut(s)	493
HaeIII	GGCC	7 cut(s)	198, 243, 564, 1076, 1099, 1915, 1928
HapII	CCGG	4 cut(s)	181, 244, 260, 726
HgaI	GACGC	4 cut(s)	170, 377, 730, 1225
HhaI	GCGC	4 cut(s)	150, 166, 259, 492
Hin1I	GRCGYC	3 cut(s)	369, 741, 1217
Hin6I	GCGC	4 cut(s)	148, 164, 257, 490
HinP1I	GCGC	4 cut(s)	148, 164, 257, 490
HincII	GTYRAC	1 cut(s)	367
HindII	GTYRAC	1 cut(s)	367
HindIII	AAGCTT	2 cut(s)	434, 1479
HinfI	GANTC	8 cut(s)	27, 37, 608, 647, 1223, 1291, 1966, 1991
HpaII	CCGG	4 cut(s)	181, 244, 260, 726
HphI	GGTGA	4 cut(s)	589, 1376, 1916, 1945
Hpy166II	GTNNAC	3 cut(s)	316, 367, 1383
Hpy8I	GTNNAC	3 cut(s)	316, 367, 1383
Hpy99I	CGWCG	2 cut(s)	164, 371
HpyAV	CCTTC	3 cut(s)	133, 1741, 1951
HpyCH4III	ACNGT	3 cut(s)	595, 1346, 1899
HpyCH4IV	ACGT	2 cut(s)	14, 1630
HpySE526I	ACGT	2 cut(s)	14, 1630
Hsp92I	GRCGYC	3 cut(s)	369, 741, 1217
HspAI	GCGC	4 cut(s)	148, 164, 257, 490
Ksp22I	TGATCA	1 cut(s)	415
LguI	GCTCTTC	1 cut(s)	197
LmnI	GCTCC	6 cut(s)	60, 118, 159, 280, 674, 982
Lsp1109I	GCAGC	5 cut(s)	295, 298, 689, 977, 1858
LweI	GCATC	7 cut(s)	135, 1401, 1561, 1729, 1816, 1945, 1956
MaeI	CTAG	3 cut(s)	1251, 1490, 2023
MaeII	ACGT	2 cut(s)	14, 1630
MaeIII	GTNAC	7 cut(s)	10, 512, 595, 815, 997, 1084, 1623
MboII	GAAGA	7 cut(s)	184, 1142, 1286, 1471, 1832, 1835, 1998
MflI	RGATCY	1 cut(s)	1594
MhlI	GDGCHC	3 cut(s)	65, 114, 123
MlyI	GAGTC	4 cut(s)	36, 1232, 1285, 1975
MspA1I	CMGCKG	2 cut(s)	289, 1138
MspCI	CTTAAG	1 cut(s)	1582
MspI	CCGG	4 cut(s)	181, 244, 260, 726
MspR9I	CCNGG	6 cut(s)	182, 245, 727, 792, 1276, 1694
Mva1269I	GAATGC	3 cut(s)	968, 1307, 1832
MvaI	CCWGG	3 cut(s)	792, 1276, 1694
MvnI	CGCG	5 cut(s)	9, 164, 257, 330, 1308
NciI	CCSGG	3 cut(s)	182, 245, 727
NcoI	CCATGG	1 cut(s)	565
NdeI	CATATG	2 cut(s)	1165, 1497
NlaIV	GGNNCC	5 cut(s)	120, 242, 466, 978, 1691
NmeAIII	GCCGAG	3 cut(s)	72, 397, 1465
NmuCI	GTSAC	5 cut(s)	10, 512, 595, 815, 1623
NotI	GCGGCCGC	1 cut(s)	1913
NsbI	TGCGCA	1 cut(s)	149
NspI	RCATGY	2 cut(s)	1288, 1922
NspV	TTCGAA	2 cut(s)	126, 871
PaeI	GCATGC	1 cut(s)	1922
PaeR7I	CTCGAG	1 cut(s)	24
PagI	TCATGA	1 cut(s)	201
PciSI	GCTCTTC	1 cut(s)	197
PcsI	WCGNNNNNNNCGW	2 cut(s)	1223, 1950
PctI	GAATGC	3 cut(s)	968, 1307, 1832
PfeI	GAWTC	4 cut(s)	37, 608, 647, 1991
PflMI	CCANNNNNTGG	1 cut(s)	277
PfoI	TCCNGGA	2 cut(s)	725, 790
PleI	GAGTC	4 cut(s)	35, 1231, 1285, 1974
PpsI	GAGTC	4 cut(s)	35, 1231, 1285, 1974
PpuMI	RGGWCCY	1 cut(s)	1690
PshBI	ATTAAT	1 cut(s)	633
Psp124BI	GAGCTC	2 cut(s)	65, 114
Psp5II	RGGWCCY	1 cut(s)	1690
Psp6I	CCWGG	3 cut(s)	790, 1274, 1692
PspFI	CCCAGC	1 cut(s)	236
PspGI	CCWGG	3 cut(s)	790, 1274, 1692
PspN4I	GGNNCC	5 cut(s)	120, 242, 466, 978, 1691
PspPI	GGNCC	6 cut(s)	178, 241, 562, 1074, 1097, 1690
PspPPI	RGGWCCY	1 cut(s)	1690
PspXI	VCTCGAGB	1 cut(s)	24
PstNI	CAGNNNCTG	1 cut(s)	905
PsuI	RGATCY	1 cut(s)	1594
RsaI	GTAC	5 cut(s)	616, 1058, 1658, 1802, 1850
RsaNI	GTAC	5 cut(s)	615, 1057, 1657, 1801, 1849
SacI	GAGCTC	2 cut(s)	65, 114
SalI	GTCGAC	1 cut(s)	365
SapI	GCTCTTC	1 cut(s)	197
Sau96I	GGNCC	6 cut(s)	178, 241, 562, 1074, 1097, 1690
ScaI	AGTACT	2 cut(s)	616, 1658
SchI	GAGTC	4 cut(s)	36, 1232, 1285, 1975
ScrFI	CCNGG	6 cut(s)	182, 245, 727, 792, 1276, 1694
SduI	GDGCHC	3 cut(s)	65, 114, 123
SfaNI	GCATC	7 cut(s)	135, 1401, 1561, 1729, 1816, 1945, 1956
SfcI	CTRYAG	1 cut(s)	1002
Sfr274I	CTCGAG	1 cut(s)	24
SfuI	TTCGAA	2 cut(s)	126, 871
SinI	GGWCC	2 cut(s)	178, 1690
SlaI	CTCGAG	1 cut(s)	24
SmlI	CTYRAG	3 cut(s)	24, 173, 1582
SmoI	CTYRAG	3 cut(s)	24, 173, 1582
SphI	GCATGC	1 cut(s)	1922
SspI	AATATT	1 cut(s)	1722
SspMI	CTAG	3 cut(s)	1251, 1490, 2023
SstI	GAGCTC	2 cut(s)	65, 114
StyD4I	CCNGG	6 cut(s)	180, 243, 725, 790, 1274, 1692
StyI	CCWWGG	4 cut(s)	138, 565, 736, 1929
TaaI	ACNGT	3 cut(s)	595, 1346, 1899
TaiI	ACGT	2 cut(s)	17, 1633
TatI	WGTACW	2 cut(s)	614, 1656
TauI	GCSGC	7 cut(s)	292, 1141, 1308, 1751, 1832, 1915, 1918
TfiI	GAWTC	4 cut(s)	37, 608, 647, 1991
TscAI	CASTG	4 cut(s)	523, 1437, 1556, 1929
TseFI	GTSAC	5 cut(s)	10, 512, 595, 815, 1623
TseI	GCWGC	6 cut(s)	283, 286, 677, 965, 1871, 2017
Tsp45I	GTSAC	5 cut(s)	10, 512, 595, 815, 1623
TspDTI	ATGAA	7 cut(s)	218, 317, 786, 961, 1514, 1723, 1755
TspGWI	ACGGA	1 cut(s)	368
TspRI	CASTG	4 cut(s)	523, 1437, 1556, 1929
Van91I	CCANNNNNTGG	1 cut(s)	277
Vha464I	CTTAAG	1 cut(s)	1582
VpaK11BI	GGWCC	2 cut(s)	178, 1690
VspI	ATTAAT	1 cut(s)	633
XapI	RAATTY	7 cut(s)	128, 623, 873, 886, 895, 1367, 1996
XceI	RCATGY	2 cut(s)	1288, 1922
XhoI	CTCGAG	1 cut(s)	24
XmiI	GTMKAC	2 cut(s)	315, 366
XspI	CTAG	3 cut(s)	1251, 1490, 2023
ZrmI	AGTACT	2 cut(s)	616, 1658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.