MD17G1262400.v1.1

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011933
Physical Location & Seq
Forward (+)
32300623 .. 32302587
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1262400.v1.1.491

Sequence Viewer

Length: 1965 bp
ATGATTGCCGCCATTTCCCTCTCAACGAACAAACCTGCCGCTTCAACCAACATAACTGTACCTTCTTCAATCCCCAACGGTCCTCAAAAACCTAGCAAACCTCTACCTTCAACCCTCAAATGGCTCGCCAAATCCGGCAAATTAGACGAGGCCCTTCGCCTGATCGAATCCTCACCCTCCAAATTAACAGCCACCCAGTCGGACATCGAGGCCTACTCCCTCCTCCTCCACACCTGCATCTCCAGAAGATCCTTAGAGCACGGCCAAAGGCTTTATCTCCAGCTTCTCCTCTCCGACGACAGGTGTGAAAACGGCATCCTTCTCCGAAACCCCACCTTGAAAAGCAAGCTCATTACGCTCTACTCCGTTTGCGGCAGAGTCGACGATGCTCGCTCTGTTTTTTATGATGGGGTTGAAGATGGTCAGATGCCGGAGTCGGTGTGGGTGGCGATGGCTATCGGGTACTTGCGAAACGGGTTCTTGGTGGAAGCATTGATTGTGTACTGTGACATGCTGAGCAGGTTAGTCCTGCCGGGTAATTTCGCTTTTTCGATGGCCTTGAAGGCGTGTGCGGAGCTGTCTGAGTTGAGGGTTGGTAGGGCGGTCCATGCCCAGATTGTGAAATCCAGTGAAGAGCCTGATCAGGTGGTGAGTAATGCTCTGCTGCGGTTGTATGCGGAGAACGGGTGTTTTGTGGATGTCTTCAAGGTGTTCGATACAATGCCTGAGAGAAATGTTGTGTCTTGGAACTCTTTGATTGCGAGTTTTGCTCGACGAGACCAAGTGTTTCAGTCATTGGATTGTTTTAGAAGGATGCAAGGACAAGGGATGGGATTCAGTTGGGTCACCCTCACGACGATTTTACCAGTTTGCGCTAGAATGACTGCACTTCATTTCGGGAAAGAGATACATGCCCAGATTGTAAAGTCCACCAAGAGACCAGATGTACCTGTACTAAACTCACTTGTCGACATGTATGCGAAATCTGGAGCAATTGATTACTGTAAGAGGGTGTTTGATGGAATGCAAAGTAAAGACTTGACATCATGGAATACTGTGCTGGCAGGGTATGCCAGCAATGGGTTTATGGAAGAGGGAATGAAATTGTTTGCTCAGATGGTAGAGTCTGGCATCCGACCAGATGGGGTAACATTTATAGCTTTGTTGTCTGGCTGTAGCCATGCGGGGCTTACTGATGAAGGACAAAGTTTATTTAGTAAAATGAAGGAGGGTTATGGCGTGTCACCAACATTTGAGCATTATGCTTGCTTGGTGGATCTGTTGGGTAGAGCCGGTAGAATTAAGGAAGCGTTAGATATAGTGGAAAACATGCCTATGAAGCCAACTGGTAGCATATGGGGATCGTTACTCAACTCATGTCGGCTCCAAGGCAATGTTTCTCTTGCAGAGCGAGCTGCAATGGAGTTGTTTGAGCTTGAACGACACAATCCTGGGAACTATGTGATGCTCTCAAACATTTATGCAAATGCAGGGATGTGGGAAGGTGTGAATAGGGTTAGGGAACTAATGAAGGATAGGGGAATAAAAAAGGAGGCTGGATGCAGTTGGATACAAATAAAGAACAAAATTCACACATTTGTAGCAGGTGGCGGTTTTGAATTTCGTAATTCAACCGAGTTTAAAAGGGTTTGGAGTGAATTGATGGATGCTATGGAAGAGGTTGGATACAACACTGACACCAGTGTTGTCCTACATGATGTAAATGAAGAAACCAAAGCAATGTGGGTTTGTGGACATAGTGAAAGAATCGCACTTATGTTCGCACTTGTCCACACTGCTGCCGGAATGCCAATTAGGATTACAAAGAACTTGCGCATTTGTGCAGATTGCCACACCTGGGTGAAAATAGTTTCAATGGTTACAGGGAGAGAGATTGTTTTGAGAGACACAAATCGCTTCCACCATTTCAAAGGAGGAGCATGCTCTTGCAAGGACTACTGGTAA

Protein Analysis

655

Amino Acids

72.75

Weight (kDa)

8.02

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 346 - 393 1.7e-11 PPR repeat family
PPR PF01535 349 - 378 1.9e-07 PPR repeat
PPR_3 PF13812 368 - 429 2.2e-06 Pentatricopeptide repeat domain
E_motif PF20431 465 - 525 4.6e-24 E motif
DYW_deaminase PF14432 562 - 654 1.1e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 242, 1595
Acc16I TGCGCA 1 cut(s) 1835
Acc36I ACCTGC 4 cut(s) 43, 242, 510, 1595
AccI GTMKAC 2 cut(s) 381, 969
AciI CCGC 9 cut(s) 9, 39, 372, 572, 602, 667, 677, 1184, 1611
AclWI GGATC 3 cut(s) 243, 1284, 1369
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 1587, 1619
AfaI GTAC 5 cut(s) 60, 464, 503, 948, 954
AfiI CCNNNNNNNGG 4 cut(s) 120, 300, 1080, 1858
AflIII ACRYGT 1 cut(s) 972
AjnI CCWGG 2 cut(s) 1450, 1856
AleI CACNNNNGTG 1 cut(s) 1859
AluBI AGCT 6 cut(s) 283, 349, 577, 1160, 1415, 1435
AluI AGCT 6 cut(s) 283, 349, 577, 1160, 1415, 1435
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 3 cut(s) 771, 931, 1899
AlwI GGATC 3 cut(s) 243, 1284, 1369
AoxI GGCC 4 cut(s) 150, 210, 262, 555
ApeKI GCWGC 3 cut(s) 664, 1415, 1799
ApoI RAATTY 2 cut(s) 1587, 1619
AspLEI GCGC 2 cut(s) 875, 1836
AspS9I GGNCC 3 cut(s) 80, 151, 604
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 5 cut(s) 165, 661, 838, 1236, 1873
AvaII GGWCC 2 cut(s) 80, 604
BbsI GAAGAC 1 cut(s) 694
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 3 cut(s) 651, 1402, 1786
BccI CCATC 9 cut(s) 401, 413, 445, 547, 823, 1013, 1111, 1136, 1657
BceAI ACGGC 2 cut(s) 277, 328
BcgI CGANNNNNNTGC 2 cut(s) 959, 993
BciT130I CCWGG 2 cut(s) 1452, 1858
BciVI GTATCC 2 cut(s) 1563, 1679
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 771, 931, 1899
BfaI CTAG 2 cut(s) 93, 876
BfmI CTRYAG 1 cut(s) 1174
BfuAI ACCTGC 4 cut(s) 43, 242, 510, 1595
BfuI GTATCC 2 cut(s) 1563, 1679
BglI GCCNNNNNGGC 1 cut(s) 563
BisI GCNGC 6 cut(s) 9, 39, 373, 665, 1416, 1800
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 6 cut(s) 10, 40, 374, 666, 1417, 1801
Bme1390I CCNGG 3 cut(s) 534, 1452, 1858
Bme18I GGWCC 2 cut(s) 80, 604
BmgT120I GGNCC 3 cut(s) 80, 151, 604
BmiI GGNNCC 1 cut(s) 1385
BmrFI CCNGG 3 cut(s) 534, 1452, 1858
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 9 cut(s) 246, 324, 376, 417, 804, 1140, 1455, 1550, 1657
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 694
BplI GAGNNNNNCTC 6 cut(s) 200, 232, 643, 675, 754, 786
BpmI CTGGAG 3 cut(s) 226, 263, 1008
Bpu1102I GCTNAGC 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 534
BsaBI GATNNNNATC 1 cut(s) 455
BsaI GGTCTC 2 cut(s) 771, 931
BsaJI CCNNGG 3 cut(s) 1387, 1451, 1857
BsaXI ACNNNNNCTCC 4 cut(s) 425, 455, 671, 701
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 300, 1080, 1858
Bse118I RCCGGY 1 cut(s) 1292
Bse1I ACTGG 6 cut(s) 196, 627, 866, 1351, 1701, 1964
Bse3DI GCAATG 4 cut(s) 1084, 1399, 1425, 1746
Bse8I GATNNNNATC 1 cut(s) 455
BseBI CCWGG 2 cut(s) 1452, 1858
BseDI CCNNGG 3 cut(s) 1387, 1451, 1857
BseGI GGATG 8 cut(s) 315, 703, 819, 834, 1131, 1500, 1565, 1672
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 4 cut(s) 120, 300, 1080, 1858
BseMI GCAATG 4 cut(s) 1084, 1399, 1425, 1746
BseMII CTCAG 4 cut(s) 506, 573, 717, 1127
BseNI ACTGG 6 cut(s) 196, 627, 866, 1351, 1701, 1964
BseRI GAGGAG 4 cut(s) 212, 215, 278, 1950
BseXI GCAGC 3 cut(s) 651, 1402, 1786
BsgI GTGCAG 2 cut(s) 870, 1863
BshFI GGCC 4 cut(s) 152, 212, 264, 557
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 5 cut(s) 135, 431, 533, 1293, 1803
BslI CCNNNNNNNGG 4 cut(s) 120, 300, 1080, 1858
BsmAI GTCTC 3 cut(s) 771, 931, 1899
BsmI GAATGC 2 cut(s) 1029, 1812
BsnI GGCC 4 cut(s) 152, 212, 264, 557
Bso31I GGTCTC 2 cut(s) 771, 931
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 5 cut(s) 162, 248, 640, 1276, 1361
Bsp1720I GCTNAGC 1 cut(s) 515
BspACI CCGC 9 cut(s) 9, 39, 372, 572, 602, 667, 677, 1184, 1611
BspANI GGCC 4 cut(s) 152, 212, 264, 557
BspCNI CTCAG 4 cut(s) 507, 574, 718, 1126
BspLI GGNNCC 1 cut(s) 1385
BspMI ACCTGC 4 cut(s) 43, 242, 510, 1595
BspPI GGATC 3 cut(s) 243, 1284, 1369
BspQI GCTCTTC 1 cut(s) 627
BspTNI GGTCTC 2 cut(s) 771, 931
BsrDI GCAATG 4 cut(s) 1084, 1399, 1425, 1746
BsrFI RCCGGY 1 cut(s) 1292
BsrI ACTGG 6 cut(s) 196, 627, 866, 1351, 1701, 1964
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 3 cut(s) 1387, 1451, 1857
BssMI GATC 5 cut(s) 162, 248, 640, 1276, 1361
BssT1I CCWWGG 1 cut(s) 1387
Bst2UI CCWGG 2 cut(s) 1452, 1858
Bst4CI ACNGT 5 cut(s) 58, 80, 506, 1004, 1057
Bst6I CTCTTC 3 cut(s) 627, 1086, 1671
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 8 cut(s) 126, 347, 391, 1062, 1075, 1267, 1413, 1942
BstDEI CTNAG 5 cut(s) 253, 515, 582, 726, 1113
BstEII GGTNACC 1 cut(s) 844
BstF5I GGATG 8 cut(s) 315, 703, 819, 834, 1131, 1500, 1565, 1672
BstHHI GCGC 2 cut(s) 875, 1836
BstKTI GATC 5 cut(s) 165, 251, 643, 1279, 1364
BstMAI GTCTC 3 cut(s) 771, 931, 1899
BstMBI GATC 5 cut(s) 162, 248, 640, 1276, 1361
BstMWI GCNNNNNNNGC 7 cut(s) 355, 563, 608, 767, 1070, 1339, 1412
BstNI CCWGG 2 cut(s) 1452, 1858
BstNSI RCATGY 5 cut(s) 514, 914, 976, 1333, 1944
BstPI GGTNACC 1 cut(s) 844
BstSCI CCNGG 3 cut(s) 532, 1450, 1856
BstSFI CTRYAG 1 cut(s) 1174
BstV1I GCAGC 3 cut(s) 651, 1402, 1786
BstV2I GAAGAC 1 cut(s) 694
BstX2I RGATCY 2 cut(s) 248, 1276
BstYI RGATCY 2 cut(s) 248, 1276
BsuI GTATCC 2 cut(s) 1563, 1679
BsuRI GGCC 4 cut(s) 152, 212, 264, 557
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 8 cut(s) 315, 703, 819, 834, 1131, 1500, 1565, 1672
BtsI GCAGTG 1 cut(s) 1794
BtsIMutI CAGTG 4 cut(s) 634, 1692, 1708, 1794
BveI ACCTGC 4 cut(s) 43, 242, 510, 1595
Cac8I GCNNGC 8 cut(s) 126, 347, 391, 1062, 1075, 1267, 1413, 1942
CfoI GCGC 2 cut(s) 875, 1836
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 3 cut(s) 80, 151, 604
Csp6I GTAC 5 cut(s) 59, 463, 502, 947, 953
CviQI GTAC 5 cut(s) 59, 463, 502, 947, 953
DdeI CTNAG 5 cut(s) 253, 515, 582, 726, 1113
DpnI GATC 5 cut(s) 164, 250, 642, 1278, 1363
DpnII GATC 5 cut(s) 162, 248, 640, 1276, 1361
DraI TTTAAA 1 cut(s) 1642
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 3 cut(s) 627, 1086, 1671
EarI CTCTTC 3 cut(s) 627, 1086, 1671
Eco130I CCWWGG 1 cut(s) 1387
Eco147I AGGCCT 1 cut(s) 212
Eco31I GGTCTC 2 cut(s) 771, 931
Eco47I GGWCC 2 cut(s) 80, 604
Eco91I GGTNACC 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 844
EcoRII CCWGG 2 cut(s) 1450, 1856
EcoT14I CCWWGG 1 cut(s) 1387
ErhI CCWWGG 1 cut(s) 1387
FauI CCCGC 1 cut(s) 1177
FauNDI CATATG 1 cut(s) 1355
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 2 cut(s) 381, 969
Fnu4HI GCNGC 6 cut(s) 9, 39, 373, 665, 1416, 1800
FokI GGATG 8 cut(s) 302, 710, 826, 841, 1118, 1507, 1572, 1679
Fsp4HI GCNGC 6 cut(s) 9, 39, 373, 665, 1416, 1800
FspBI CTAG 2 cut(s) 93, 876
FspI TGCGCA 1 cut(s) 1835
GlaI GCGC 2 cut(s) 874, 1835
GluI GCNGC 6 cut(s) 9, 39, 373, 665, 1416, 1800
GsuI CTGGAG 3 cut(s) 226, 263, 1008
HaeIII GGCC 4 cut(s) 152, 212, 264, 557
HapII CCGG 5 cut(s) 135, 431, 533, 1293, 1803
HhaI GCGC 2 cut(s) 875, 1836
Hin6I GCGC 2 cut(s) 873, 1834
HinP1I GCGC 2 cut(s) 873, 1834
HincII GTYRAC 2 cut(s) 382, 970
HindII GTYRAC 2 cut(s) 382, 970
HinfI GANTC 6 cut(s) 167, 378, 434, 834, 1124, 1767
HpaII CCGG 5 cut(s) 135, 431, 533, 1293, 1803
HphI GGTGA 5 cut(s) 165, 661, 838, 1236, 1873
Hpy166II GTNNAC 6 cut(s) 382, 502, 930, 970, 1754, 1792
Hpy188I TCNGA 7 cut(s) 202, 295, 326, 426, 583, 1116, 1136
Hpy188III TCNNGA 4 cut(s) 243, 853, 898, 987
Hpy8I GTNNAC 6 cut(s) 382, 502, 930, 970, 1754, 1792
Hpy99I CGWCG 4 cut(s) 299, 386, 777, 859
HpyCH4III ACNGT 5 cut(s) 58, 80, 506, 1004, 1057
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 563, 608, 767, 1070, 1339, 1412
HpyF3I CTNAG 5 cut(s) 253, 515, 582, 726, 1113
HspAI GCGC 2 cut(s) 873, 1834
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 5 cut(s) 162, 248, 640, 1276, 1361
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 4 cut(s) 574, 989, 1389, 1937
Lsp1109I GCAGC 3 cut(s) 651, 1402, 1786
LweI GCATC 9 cut(s) 246, 324, 376, 417, 804, 1140, 1455, 1550, 1657
MaeI CTAG 2 cut(s) 93, 876
MaeIII GTNAC 6 cut(s) 506, 844, 1147, 1242, 1365, 1879
MalI GATC 5 cut(s) 164, 250, 642, 1278, 1363
MboI GATC 5 cut(s) 162, 248, 640, 1276, 1361
MboII GAAGA 8 cut(s) 57, 258, 428, 644, 694, 1103, 1688, 1739
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 2 cut(s) 248, 1276
MhlI GDGCHC 1 cut(s) 261
MlyI GAGTC 3 cut(s) 387, 443, 1133
MmeI TCCRAC 5 cut(s) 180, 318, 1159, 1547, 1663
MseI TTAA 3 cut(s) 185, 1302, 1641
MslI CAYNNNNRTG 2 cut(s) 1334, 1859
MspI CCGG 5 cut(s) 135, 431, 533, 1293, 1803
MspR9I CCNGG 3 cut(s) 534, 1452, 1858
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 2 cut(s) 1029, 1812
MvaI CCWGG 2 cut(s) 1452, 1858
MwoI GCNNNNNNNGC 7 cut(s) 355, 563, 608, 767, 1070, 1339, 1412
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 1355
NdeII GATC 5 cut(s) 162, 248, 640, 1276, 1361
NlaIV GGNNCC 1 cut(s) 1385
NmuCI GTSAC 3 cut(s) 506, 844, 1242
NsbI TGCGCA 1 cut(s) 1835
NspI RCATGY 5 cut(s) 514, 914, 976, 1333, 1944
OliI CACNNNNGTG 1 cut(s) 1859
PaeI GCATGC 1 cut(s) 1944
PaqCI CACCTGC 2 cut(s) 242, 1595
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 2 cut(s) 1029, 1812
PfeI GAWTC 3 cut(s) 167, 834, 1767
PkrI GCNGC 6 cut(s) 10, 40, 374, 666, 1417, 1801
PleI GAGTC 3 cut(s) 386, 442, 1132
PpsI GAGTC 3 cut(s) 386, 442, 1132
PscI ACATGT 1 cut(s) 972
Psp6I CCWGG 2 cut(s) 1450, 1856
PspEI GGTNACC 1 cut(s) 844
PspGI CCWGG 2 cut(s) 1450, 1856
PspN4I GGNNCC 1 cut(s) 1385
PspPI GGNCC 3 cut(s) 80, 151, 604
PsuI RGATCY 2 cut(s) 248, 1276
RsaI GTAC 5 cut(s) 60, 464, 503, 948, 954
RsaNI GTAC 5 cut(s) 59, 463, 502, 947, 953
RseI CAYNNNNRTG 2 cut(s) 1334, 1859
SalI GTCGAC 2 cut(s) 380, 968
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 185, 1302, 1641
SatI GCNGC 6 cut(s) 9, 39, 373, 665, 1416, 1800
Sau3AI GATC 5 cut(s) 162, 248, 640, 1276, 1361
Sau96I GGNCC 3 cut(s) 80, 151, 604
SchI GAGTC 3 cut(s) 387, 443, 1133
ScrFI CCNGG 3 cut(s) 534, 1452, 1858
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 9 cut(s) 246, 324, 376, 417, 804, 1140, 1455, 1550, 1657
SfcI CTRYAG 1 cut(s) 1174
SinI GGWCC 2 cut(s) 80, 604
SmiMI CAYNNNNRTG 2 cut(s) 1334, 1859
SphI GCATGC 1 cut(s) 1944
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 9 cut(s) 9, 39, 372, 572, 602, 667, 677, 1184, 1611
SspMI CTAG 2 cut(s) 93, 876
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 3 cut(s) 532, 1450, 1856
StyI CCWWGG 1 cut(s) 1387
TaaI ACNGT 5 cut(s) 58, 80, 506, 1004, 1057
TaqI TCGA 7 cut(s) 165, 207, 381, 551, 714, 772, 969
TatI WGTACW 2 cut(s) 501, 952
TauI GCSGC 3 cut(s) 11, 41, 375
TfiI GAWTC 3 cut(s) 167, 834, 1767
Tru1I TTAA 3 cut(s) 185, 1302, 1641
Tru9I TTAA 3 cut(s) 185, 1302, 1641
TscAI CASTG 4 cut(s) 634, 1699, 1708, 1801
TseFI GTSAC 3 cut(s) 506, 844, 1242
TseI GCWGC 3 cut(s) 664, 1415, 1799
Tsp45I GTSAC 3 cut(s) 506, 844, 1242
TspDTI ATGAA 7 cut(s) 881, 1115, 1212, 1238, 1352, 1544, 1740
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 4 cut(s) 634, 1699, 1708, 1801
VpaK11BI GGWCC 2 cut(s) 80, 604
XapI RAATTY 2 cut(s) 1587, 1619
XceI RCATGY 5 cut(s) 514, 914, 976, 1333, 1944
XmiI GTMKAC 2 cut(s) 381, 969
XspI CTAG 2 cut(s) 93, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.