MD17G1270600.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008565
Physical Location & Seq
Forward (+)
33145870 .. 33154308
8439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1270600.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGCGGAGGGCCAAAACCCCTGTCCTCCTTGTCGCCGCAACTGGCCTCACCTTCCACGCCTTCAACTCTGATTTCCTCTCGTCCTCCTCCGCCGATATCCTCCCATCATTCCCAGAACACCTCCGCACACCAATTCACGGCGTCGTTCGGTCTTCCCGTGCAATCTCCACCATTGCGTTGACTGCCGTTGACTACAAGCTTACTCTGCGTGGAATCCCAGAGGACTCCGATGAGTACCGTCAGAAGCTATCCCAGGTTCATAGTCGTTCCGCTTCGAGAATTCTGAAGCTGTGTGAGGCCAATAGAGGATTTTATGTGAAAGCCGGTCAATTTGTGGCCGCTCTGCGACAGGTTCCGAAAGAGTACTCATCAACCCTTTCCTCATTGCAGGATCAGGCAGTTCCTTGTCATTTGAAAGCAATTAAAGAAGTGCTGATCCGCAATCTGGGACACAAGCTGTCAGATGTGTTTCTGTCACTTGATGAGCTTCCCATAGCCGCTGCATCTATTGCTCAAGTACACAGGGGAGTTCTTAAGGATCATCAAGAAGTAGCAGTTAAGGTGCAGTACCCAGGTCTGGAGCAGCACATGAAAATAGATACAGCAACCATGTATTTCCTTTCAAAATCCCTTACATTGTTTTTCCCGGAATACAGATTTGACTGGCTGGTATCGGAGTTCGTACTTGCTATATCTCTCGAACTTGACTTTATTCAGGAGGCTAGGAATTCTGAGACAACTGCCACTAACTTTGCAAACAACAAATGGGTCAAGATTCCTCGTATATTTTGGGACTTGACCACTACTCAAGTGCTAACAATGGAATTCTGTACGGGCCACAAGGTTGATGATGTACAGTTTCTGGAGGAAAGGAGTATAAACCCAATGAAGGTAGCAGAAGTATTATTGGAAGCGTTTGCTGAAATGATTTTTATCCATGGTTTCCTGCATGGAGATCCACACCCTGGTAACATATTAGTTTCACCAGAAGGTCCCAATGGTTTTTCTCTGGTTCTTCTAGACCATGGAATATACAAACAATTAGATGAAGGATTCAGGTTGGACTACTGTCAACTCTGGAAGGCTTTAATTCTTCTAGACTCTAAAAAATTGCATCATTTGGGTGAAAGGTTTGGTGTTGGGAAGTATTCTAGATACTTTCCTGTCATTTTTACGGGAAGAACTATTGACAGTAAATCGGCTCTTGGGAAGACAATGTCAGTTGAAGAAAAAAGTAATCTAAAGCAAGAGTTGAAGTCCCTAAAGATGGAGGACATATCTTCATTTATGGAATCCTTGCCATCTGACTTCCTCACAATATTGCGCACTGACGGGCTACTTAGGTCTATCGTTAGCAAGTTGGGTGCTCCACAAAGAGTGAGGTTACTAGCCTATGGAAAATATGCATTATATGGTCTTTCTCCAAAGTTGAATCCTGAATCTGATTTTTCTATGGAAGTGAAGTTATCCCGATTGAAGGCAAAAGTGAGCTACCTTTGGTTAAGGCTTATTCTCGGGGTAATCGAGCTCCGTTTGCAGTTGGGAAAGGTCAAGCTTCTGCTGCACATTTTGTATGAAAAGTTGGGTGGTGCTACGAGGAGAATTTTGCTTATTTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

541

Amino Acids

60.95

Weight (kDa)

9.15

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 129 - 372 2.8e-84 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1325
AccB7I CCANNNNNTGG 1 cut(s) 965
AccBSI CCGCTC 1 cut(s) 341
AciI CCGC 8 cut(s) 4, 36, 90, 124, 270, 339, 439, 498
AclWI GGATC 4 cut(s) 399, 430, 546, 950
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 4 cut(s) 279, 727, 824, 1602
AcuI CTGAAG 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 7 cut(s) 236, 365, 519, 569, 684, 832, 855
AfiI CCNNNNNNNGG 7 cut(s) 137, 445, 577, 889, 965, 1267, 1477
AflII CTTAAG 1 cut(s) 533
AgsI TTSAA 7 cut(s) 64, 415, 624, 1226, 1255, 1432, 1477
AjnI CCWGG 3 cut(s) 252, 571, 964
AluBI AGCT 8 cut(s) 199, 247, 289, 457, 487, 1491, 1528, 1555
AluI AGCT 8 cut(s) 199, 247, 289, 457, 487, 1491, 1528, 1555
Alw21I GWGCWC 2 cut(s) 1369, 1530
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 4 cut(s) 399, 430, 546, 950
AlwNI CAGNNNCTG 1 cut(s) 862
Ama87I CYCGRG 1 cut(s) 1514
AoxI GGCC 5 cut(s) 9, 43, 297, 336, 835
ApeKI GCWGC 3 cut(s) 500, 583, 1561
ApoI RAATTY 4 cut(s) 279, 727, 824, 1602
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspLEI GCGC 1 cut(s) 1326
AspS9I GGNCC 3 cut(s) 9, 835, 992
AsuC2I CCSGG 1 cut(s) 647
AsuHPI GGTGA 3 cut(s) 40, 975, 1136
AvaI CYCGRG 1 cut(s) 1514
AvaII GGWCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 1530
BbsI GAAGAC 2 cut(s) 144, 1217
Bbv12I GWGCWC 2 cut(s) 1369, 1530
BbvI GCAGC 3 cut(s) 487, 595, 1548
BccI CCATC 3 cut(s) 112, 1261, 1309
BceAI ACGGC 2 cut(s) 154, 170
BciT130I CCWGG 3 cut(s) 254, 573, 966
BcnI CCSGG 1 cut(s) 647
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 5 cut(s) 723, 1019, 1097, 1152, 1388
BfrI CTTAAG 1 cut(s) 533
BisI GCNGC 6 cut(s) 36, 339, 498, 501, 584, 1562
BlsI GCNGC 6 cut(s) 37, 340, 499, 502, 585, 1563
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 254, 573, 647, 966
Bme18I GGWCC 1 cut(s) 992
BmeT110I CYCGRG 1 cut(s) 1514
BmgT120I GGNCC 3 cut(s) 9, 835, 992
BmiI GGNNCC 2 cut(s) 354, 994
BmrFI CCNGG 4 cut(s) 254, 573, 647, 966
BmsI GCATC 2 cut(s) 512, 1123
BoxI GACNNNNGTC 1 cut(s) 1068
BpiI GAAGAC 2 cut(s) 144, 1217
BpmI CTGGAG 2 cut(s) 599, 884
BpuEI CTTGAG 2 cut(s) 498, 792
BpuMI CCSGG 1 cut(s) 647
BsaBI GATNNNNATC 1 cut(s) 932
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 5 cut(s) 252, 571, 937, 964, 1024
Bsc4I CCNNNNNNNGG 7 cut(s) 137, 445, 577, 889, 965, 1267, 1477
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 46, 668
Bse3DI GCAATG 2 cut(s) 171, 383
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 3 cut(s) 254, 573, 966
BseDI CCNNGG 5 cut(s) 252, 571, 937, 964, 1024
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 7 cut(s) 137, 445, 577, 889, 965, 1267, 1477
BseMI GCAATG 2 cut(s) 171, 383
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 2 cut(s) 46, 668
BseRI GAGGAG 2 cut(s) 76, 1612
BseXI GCAGC 3 cut(s) 487, 595, 1548
BsgI GTGCAG 2 cut(s) 584, 1547
BshFI GGCC 5 cut(s) 11, 45, 299, 338, 837
BsiHKAI GWGCWC 2 cut(s) 1369, 1530
BsiHKCI CYCGRG 1 cut(s) 1514
BsiSI CCGG 2 cut(s) 324, 647
BslFI GGGAC 4 cut(s) 462, 806, 978, 1243
BslI CCNNNNNNNGG 7 cut(s) 137, 445, 577, 889, 965, 1267, 1477
BsmAI GTCTC 1 cut(s) 728
BsmFI GGGAC 4 cut(s) 462, 806, 978, 1243
BsnI GGCC 5 cut(s) 11, 45, 299, 338, 837
BsoBI CYCGRG 1 cut(s) 1514
Bsp1286I GDGCHC 2 cut(s) 1369, 1530
Bsp1407I TGTACA 1 cut(s) 853
Bsp143I GATC 4 cut(s) 391, 435, 538, 955
Bsp19I CCATGG 2 cut(s) 937, 1024
BspACI CCGC 8 cut(s) 4, 36, 90, 124, 270, 339, 439, 498
BspANI GGCC 5 cut(s) 11, 45, 299, 338, 837
BspCNI CTCAG 1 cut(s) 724
BspLI GGNNCC 2 cut(s) 354, 994
BspPI GGATC 4 cut(s) 399, 430, 546, 950
BspTI CTTAAG 1 cut(s) 533
BsrBI CCGCTC 1 cut(s) 341
BsrDI GCAATG 2 cut(s) 171, 383
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 2 cut(s) 46, 668
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 5 cut(s) 252, 571, 937, 964, 1024
BssMI GATC 4 cut(s) 391, 435, 538, 955
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 937, 1024
Bst2UI CCWGG 3 cut(s) 254, 573, 966
Bst4CI ACNGT 4 cut(s) 239, 858, 1070, 1193
BstACI GRCGYC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 853
BstDEI CTNAG 3 cut(s) 732, 1340, 1620
BstDSI CCRYGG 2 cut(s) 937, 1024
BstHHI GCGC 1 cut(s) 1326
BstKTI GATC 4 cut(s) 394, 438, 541, 958
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 4 cut(s) 391, 435, 538, 955
BstMWI GCNNNNNNNGC 5 cut(s) 182, 205, 509, 1534, 1561
BstNI CCWGG 3 cut(s) 254, 573, 966
BstPAI GACNNNNGTC 1 cut(s) 1068
BstSCI CCNGG 4 cut(s) 252, 571, 645, 964
BstV1I GCAGC 3 cut(s) 487, 595, 1548
BstV2I GAAGAC 2 cut(s) 144, 1217
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuRI GGCC 5 cut(s) 11, 45, 299, 338, 837
BtgI CCRYGG 2 cut(s) 937, 1024
BtsIMutI CAGTG 1 cut(s) 1326
CaiI CAGNNNCTG 1 cut(s) 862
CfoI GCGC 1 cut(s) 1326
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 3 cut(s) 9, 835, 992
CseI GACGC 1 cut(s) 130
Csp6I GTAC 7 cut(s) 235, 364, 518, 568, 683, 831, 854
CspCI CAANNNNNGTGG 2 cut(s) 733, 768
CviAII CATG 5 cut(s) 589, 610, 938, 950, 1025
CviQI GTAC 7 cut(s) 235, 364, 518, 568, 683, 831, 854
DdeI CTNAG 3 cut(s) 732, 1340, 1620
DpnI GATC 4 cut(s) 393, 437, 540, 957
DpnII GATC 4 cut(s) 391, 435, 538, 955
EaeI YGGCCR 1 cut(s) 336
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 1528
Eco130I CCWWGG 2 cut(s) 937, 1024
Eco24I GRGCYC 1 cut(s) 1530
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 992
Eco53kI GAGCTC 1 cut(s) 1528
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 1514
EcoICRI GAGCTC 1 cut(s) 1528
EcoO109I RGGNCCY 1 cut(s) 992
EcoRI GAATTC 3 cut(s) 279, 727, 824
EcoRII CCWGG 3 cut(s) 252, 571, 964
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 937, 1024
EcoT22I ATGCAT 1 cut(s) 1408
EcoT38I GRGCYC 1 cut(s) 1530
ErhI CCWWGG 2 cut(s) 937, 1024
FaeI CATG 5 cut(s) 592, 613, 941, 953, 1028
FaqI GGGAC 4 cut(s) 462, 806, 978, 1243
FatI CATG 5 cut(s) 588, 609, 937, 949, 1024
Fnu4HI GCNGC 6 cut(s) 36, 339, 498, 501, 584, 1562
FriOI GRGCYC 1 cut(s) 1530
Fsp4HI GCNGC 6 cut(s) 36, 339, 498, 501, 584, 1562
FspBI CTAG 5 cut(s) 723, 1019, 1097, 1152, 1388
FspI TGCGCA 1 cut(s) 1325
GlaI GCGC 1 cut(s) 1325
GluI GCNGC 6 cut(s) 36, 339, 498, 501, 584, 1562
GsuI CTGGAG 2 cut(s) 599, 884
HaeIII GGCC 5 cut(s) 11, 45, 299, 338, 837
HapII CCGG 2 cut(s) 324, 647
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 1326
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 5 cut(s) 592, 613, 941, 953, 1028
Hin6I GCGC 1 cut(s) 1324
HinP1I GCGC 1 cut(s) 1324
HincII GTYRAC 3 cut(s) 180, 190, 1073
HindII GTYRAC 3 cut(s) 180, 190, 1073
HindIII AAGCTT 2 cut(s) 197, 1553
HinfI GANTC 8 cut(s) 213, 224, 775, 1053, 1100, 1292, 1432, 1439
HpaII CCGG 2 cut(s) 324, 647
HphI GGTGA 3 cut(s) 40, 975, 1136
Hpy166II GTNNAC 4 cut(s) 180, 190, 520, 1073
Hpy8I GTNNAC 4 cut(s) 180, 190, 520, 1073
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 7 cut(s) 61, 70, 883, 983, 1043, 1075, 1471
HpyCH4III ACNGT 4 cut(s) 239, 858, 1070, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 205, 509, 1534, 1561
HpyF3I CTNAG 3 cut(s) 732, 1340, 1620
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 5 cut(s) 592, 613, 941, 953, 1028
HspAI GCGC 1 cut(s) 1324
Kzo9I GATC 4 cut(s) 391, 435, 538, 955
LmnI GCTCC 3 cut(s) 580, 1372, 1533
Lsp1109I GCAGC 3 cut(s) 487, 595, 1548
LweI GCATC 2 cut(s) 512, 1123
MaeI CTAG 5 cut(s) 723, 1019, 1097, 1152, 1388
MaeIII GTNAC 3 cut(s) 474, 968, 1383
MalI GATC 4 cut(s) 393, 437, 540, 957
MbiI CCGCTC 1 cut(s) 341
MboI GATC 4 cut(s) 391, 435, 538, 955
MboII GAAGA 7 cut(s) 144, 1007, 1085, 1191, 1222, 1238, 1272
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 2 cut(s) 1369, 1530
MlyI GAGTC 2 cut(s) 218, 1094
MmeI TCCRAC 1 cut(s) 1041
Mph1103I ATGCAT 1 cut(s) 1408
MseI TTAA 5 cut(s) 423, 534, 558, 1088, 1502
MslI CAYNNNNRTG 1 cut(s) 1122
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 2 cut(s) 324, 647
MspR9I CCNGG 4 cut(s) 254, 573, 647, 966
MvaI CCWGG 3 cut(s) 254, 573, 966
MwoI GCNNNNNNNGC 5 cut(s) 182, 205, 509, 1534, 1561
NciI CCSGG 1 cut(s) 647
NcoI CCATGG 2 cut(s) 937, 1024
NdeII GATC 4 cut(s) 391, 435, 538, 955
NlaIII CATG 5 cut(s) 592, 613, 941, 953, 1028
NlaIV GGNNCC 2 cut(s) 354, 994
NmuCI GTSAC 1 cut(s) 474
NsbI TGCGCA 1 cut(s) 1325
NsiI ATGCAT 1 cut(s) 1408
PcsI WCGNNNNNNNCGW 2 cut(s) 154, 1521
PfeI GAWTC 6 cut(s) 213, 775, 1053, 1292, 1432, 1439
PflFI GACNNNGTC 1 cut(s) 1216
PflMI CCANNNNNTGG 1 cut(s) 965
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 6 cut(s) 37, 340, 499, 502, 585, 1563
PleI GAGTC 2 cut(s) 218, 1094
PpsI GAGTC 2 cut(s) 218, 1094
PpuMI RGGWCCY 1 cut(s) 992
PshAI GACNNNNGTC 1 cut(s) 1068
Psp124BI GAGCTC 1 cut(s) 1530
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 3 cut(s) 252, 571, 964
PspGI CCWGG 3 cut(s) 252, 571, 964
PspN4I GGNNCC 2 cut(s) 354, 994
PspPI GGNCC 3 cut(s) 9, 835, 992
PspPPI RGGWCCY 1 cut(s) 992
PstNI CAGNNNCTG 1 cut(s) 862
PsuI RGATCY 1 cut(s) 955
PsyI GACNNNGTC 1 cut(s) 1216
RsaI GTAC 7 cut(s) 236, 365, 519, 569, 684, 832, 855
RsaNI GTAC 7 cut(s) 235, 364, 518, 568, 683, 831, 854
RseI CAYNNNNRTG 1 cut(s) 1122
SacI GAGCTC 1 cut(s) 1530
SaqAI TTAA 5 cut(s) 423, 534, 558, 1088, 1502
SatI GCNGC 6 cut(s) 36, 339, 498, 501, 584, 1562
Sau3AI GATC 4 cut(s) 391, 435, 538, 955
Sau96I GGNCC 3 cut(s) 9, 835, 992
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 2 cut(s) 218, 1094
ScrFI CCNGG 4 cut(s) 254, 573, 647, 966
SduI GDGCHC 2 cut(s) 1369, 1530
SfaNI GCATC 2 cut(s) 512, 1123
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 1 cut(s) 1122
SmlI CTYRAG 3 cut(s) 513, 533, 807
SmoI CTYRAG 3 cut(s) 513, 533, 807
SsiI CCGC 8 cut(s) 4, 36, 90, 124, 270, 339, 439, 498
SspI AATATT 1 cut(s) 1320
SspMI CTAG 5 cut(s) 723, 1019, 1097, 1152, 1388
SstI GAGCTC 1 cut(s) 1530
StyD4I CCNGG 4 cut(s) 252, 571, 645, 964
StyI CCWWGG 2 cut(s) 937, 1024
TaaI ACNGT 4 cut(s) 239, 858, 1070, 1193
TaqI TCGA 3 cut(s) 275, 699, 1524
TaqII GACCGA 1 cut(s) 138
TatI WGTACW 3 cut(s) 363, 517, 853
TauI GCSGC 3 cut(s) 38, 341, 500
TfiI GAWTC 6 cut(s) 213, 775, 1053, 1292, 1432, 1439
Tru1I TTAA 5 cut(s) 423, 534, 558, 1088, 1502
Tru9I TTAA 5 cut(s) 423, 534, 558, 1088, 1502
TscAI CASTG 1 cut(s) 1333
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 3 cut(s) 500, 583, 1561
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 6 cut(s) 248, 605, 902, 1062, 1272, 1590
TspGWI ACGGA 1 cut(s) 1520
TspRI CASTG 1 cut(s) 1333
Tth111I GACNNNGTC 1 cut(s) 1216
Van91I CCANNNNNTGG 1 cut(s) 965
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 992
XapI RAATTY 4 cut(s) 279, 727, 824, 1602
XbaI TCTAGA 3 cut(s) 1018, 1096, 1151
XspI CTAG 5 cut(s) 723, 1019, 1097, 1152, 1388
ZrmI AGTACT 1 cut(s) 365
Zsp2I ATGCAT 1 cut(s) 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.