Rh3AG291300

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008565
Physical Location & Seq
Reverse (-)
35202810 .. 35214133
11324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG291300.1

Sequence Viewer

Length: 1677 bp
ATGCGGAAGCTCAAAACCACAGTTTGCCTAATCACAGCCACTACTGCTGGCCTCACCTTCCATGCCTTCAACCCTAATTCCCTCTCCGATTCCTCCCCAGACTTTCCCGACAAAATTCGGACAGCCGTTCACGGCGTCGTTCGCTCTTCCCGCGCAATCTCCACCATTGCATTGACTGCCGTTGACTACAAGTTCACCTTGCGTGGACTCCCCGTGGACTCAGATGAGTACCGTCGCAAGCTATCTGAGGTTCATTTTCGATCAGCTTGGAGAATTCTGAAATTGTGTGAAGCCAACAAAGGATTTTATGTGAAAGCTGGTCAATTTGTTGCAGCTCTGCGTCAGGTTCCAAAAGAATACTCATCAACTCTTTCCTCATTACAGGATCAGGCAGTTCCTTGTCACTTTAAAGCTATAGAAGAAGTACTAATTCATAATCTGGGGCACAACCTGTCAGATACGTTTTTGTCACTGGATGAACAACCCTTAGCTGCTGCATCAATTGCTCAGGTACACAAAGCAGTTCTGAAGGATCATCAAGCAGTAGCAATCAAGGTGCAGTACCCTGGTCTGGAGCAGCAAATGAAACTAGATACAACAACAATGTATTTCATTTCAAAATCCATTACATGGTTTTTCCCGGAGTACAGATTTGGATGGCTGGTAGCAGAGTTTGTGCAGGCTATCTCTCTTGAGCTTGATTTTATTCAAGAAGCTGGAAACTCTGAGAGAACTGCCAATAATTTCAAAAACAACAAATGGGTCAAGGTTCCTCATGTATTTTGGGATTTGACAACTAAACAAGTTCTGACGATGGAATTCTGTACTGGACACAAGGTTGATGATGTAGGCTATCTGAAGGAAAGGAAAATAGATCCAGTGAAGGTGGCCAAAGTGTTGTTGGAAATGTTTGCTGAAATGATTTTTATCCATGGTTTCTTGCACGGAGATCCGCACCCTGGTAATATATTAGTTTCTCCAGAAGGGCAGAATGGCTTTTCTTTGGTTCTTCTAGATCATGGAATATACAAACAACTCAATGATAAATTTAGGTTGGACTACTGTCAGCTCTGGAAGGCTCTAATTCTTCTCGACTCAAAAAAGTTGCAGCATTTGGGGGAGCAGTTTGGTGTTGCAAAGTATTCTAAATACTTTCCTGTCATTTTTACAGGAAGAACTGTTGACAGTACATCGGCTCTTGGGAAAGGAATGTCAACTAAAGAGAGAAGGAAATTAAAGCAAGAGCTGCAGTCCCTAAAGATGGAGGACATATCTTCATTTATGGAATCTTTACCTTCTGACTTCCTCACAATATTGCGTACAGACGGACTATTGAGGTCTATTGCTAGTAAGTTGGGCGCTTCACAAAGAGTCAGGATACTAGCCTATGCCAAATATGCACTATATGGTCTTTCCCCAAAGTTGAATCCAGAATCTGACAATGTTATTAAAGTAAAGTTCTCACAATTGACAGCATATTTGAGTTACCTTCAGTTAAGGCTTATTCTTGGTAAGAATGCATTTGATCAACCATTTAACAGTAACCATGTTGTCAAGAACGGTGACTTAAGTTCTGTTGATGTTTCAGAGGTCCTAGAGCTGCTTTCTTGGTTAGCAAAGGTCAAGCAGTTTCTATTTAATTTTTATGAAAAGACAGGTGGCGCCCTGGTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

558

Amino Acids

63.06

Weight (kDa)

9.16

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 127 - 371 1.8e-86 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1661
AccB7I CCANNNNNTGG 1 cut(s) 630
AccII CGCG 1 cut(s) 153
AciI CCGC 3 cut(s) 4, 151, 953
AclWI GGATC 4 cut(s) 393, 540, 869, 944
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 4 cut(s) 114, 273, 818, 1046
AcuI CTGAAG 3 cut(s) 548, 878, 1475
AcyI GRCGYC 2 cut(s) 135, 1662
AfaI GTAC 8 cut(s) 230, 426, 513, 563, 647, 826, 1189, 1321
AfiI CCNNNNNNNGG 4 cut(s) 571, 630, 959, 1261
AflII CTTAAG 1 cut(s) 1567
AgsI TTSAA 5 cut(s) 70, 618, 710, 748, 1426
AjnI CCWGG 3 cut(s) 565, 958, 1665
AlwI GGATC 4 cut(s) 393, 540, 869, 944
AlwNI CAGNNNCTG 1 cut(s) 1436
AoxI GGCC 2 cut(s) 49, 888
ApeKI GCWGC 7 cut(s) 332, 491, 494, 577, 1108, 1246, 1600
ApoI RAATTY 4 cut(s) 114, 273, 818, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 1462, 1494
AspLEI GCGC 3 cut(s) 155, 1361, 1664
AspS9I GGNCC 1 cut(s) 1591
AsuC2I CCSGG 1 cut(s) 641
AsuHPI GGTGA 3 cut(s) 46, 187, 1574
AvaII GGWCC 1 cut(s) 1591
BaeGI GKGCMC 1 cut(s) 447
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 1 cut(s) 1661
BbvI GCAGC 7 cut(s) 344, 478, 481, 589, 1120, 1233, 1587
BccI CCATC 3 cut(s) 651, 808, 1255
BceAI ACGGC 3 cut(s) 110, 148, 164
BciT130I CCWGG 3 cut(s) 567, 960, 1667
BciVI GTATCC 1 cut(s) 1371
BclI TGATCA 1 cut(s) 1525
BcnI CCSGG 1 cut(s) 641
BfaI CTAG 5 cut(s) 590, 1013, 1347, 1382, 1595
BfmI CTRYAG 2 cut(s) 414, 1247
BfoI RGCGCY 2 cut(s) 1362, 1665
BfrI CTTAAG 1 cut(s) 1567
BfuI GTATCC 1 cut(s) 1371
BisI GCNGC 7 cut(s) 333, 492, 495, 578, 1109, 1247, 1601
BlsI GCNGC 7 cut(s) 334, 493, 496, 579, 1110, 1248, 1602
BmcAI AGTACT 1 cut(s) 426
Bme1390I CCNGG 4 cut(s) 567, 641, 960, 1667
Bme18I GGWCC 1 cut(s) 1591
BmgT120I GGNCC 1 cut(s) 1591
BmiI GGNNCC 3 cut(s) 348, 771, 1663
BmrFI CCNGG 4 cut(s) 567, 641, 960, 1667
BmsI GCATC 1 cut(s) 506
BoxI GACNNNNGTC 1 cut(s) 1062
BpmI CTGGAG 2 cut(s) 593, 963
Bpu10I CCTNAGC 2 cut(s) 487, 507
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 641
BsaBI GATNNNNATC 1 cut(s) 926
BsaHI GRCGYC 2 cut(s) 135, 1662
BsaJI CCNNGG 5 cut(s) 213, 565, 931, 958, 1665
Bsc4I CCNNNNNNNGG 4 cut(s) 571, 630, 959, 1261
Bse1I ACTGG 3 cut(s) 477, 832, 878
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 926
BseBI CCWGG 3 cut(s) 567, 960, 1667
BseDI CCNNGG 5 cut(s) 213, 565, 931, 958, 1665
BseGI GGATG 2 cut(s) 481, 662
BseJI GATNNNNATC 1 cut(s) 926
BseLI CCNNNNNNNGG 4 cut(s) 571, 630, 959, 1261
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 4 cut(s) 234, 237, 521, 717
BseNI ACTGG 3 cut(s) 477, 832, 878
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 7 cut(s) 344, 478, 481, 589, 1120, 1233, 1587
BsgI GTGCAG 2 cut(s) 578, 698
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 51, 890
BshNI GGYRCC 1 cut(s) 1661
BsiSI CCGG 1 cut(s) 641
BslFI GGGAC 1 cut(s) 1237
BslI CCNNNNNNNGG 4 cut(s) 571, 630, 959, 1261
BsmFI GGGAC 1 cut(s) 1237
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 2 cut(s) 51, 890
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 3 cut(s) 4, 151, 953
BspANI GGCC 2 cut(s) 51, 890
BspCNI CTCAG 4 cut(s) 233, 238, 520, 718
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 3 cut(s) 348, 771, 1663
BspMAI CTGCAG 1 cut(s) 1251
BspPI GGATC 4 cut(s) 393, 540, 869, 944
BspQI GCTCTTC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 1661
BspTI CTTAAG 1 cut(s) 1567
BsrDI GCAATG 1 cut(s) 165
BsrI ACTGG 3 cut(s) 477, 832, 878
BssECI CCNNGG 5 cut(s) 213, 565, 931, 958, 1665
BssMI GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
BssNI GRCGYC 2 cut(s) 135, 1662
BssT1I CCWWGG 1 cut(s) 931
Bst2UI CCWGG 3 cut(s) 567, 960, 1667
Bst4CI ACNGT 7 cut(s) 22, 233, 1064, 1180, 1187, 1541, 1562
Bst6I CTCTTC 1 cut(s) 151
BstACI GRCGYC 2 cut(s) 135, 1662
BstAFI CTTAAG 1 cut(s) 1567
BstAPI GCANNNNNTGC 3 cut(s) 176, 503, 1246
BstC8I GCNNGC 3 cut(s) 49, 239, 681
BstDEI CTNAG 5 cut(s) 220, 246, 487, 507, 726
BstDSI CCRYGG 2 cut(s) 213, 931
BstF5I GGATG 2 cut(s) 481, 662
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 2 cut(s) 1362, 1665
BstHHI GCGC 3 cut(s) 155, 1361, 1664
BstKTI GATC 7 cut(s) 263, 388, 535, 877, 952, 1018, 1528
BstMBI GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
BstMWI GCNNNNNNNGC 7 cut(s) 44, 141, 150, 176, 503, 1246, 1397
BstNI CCWGG 3 cut(s) 567, 960, 1667
BstPAI GACNNNNGTC 1 cut(s) 1062
BstSCI CCNGG 4 cut(s) 565, 639, 958, 1665
BstSFI CTRYAG 2 cut(s) 414, 1247
BstSLI GKGCMC 1 cut(s) 447
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 7 cut(s) 344, 478, 481, 589, 1120, 1233, 1587
BstX2I RGATCY 2 cut(s) 874, 949
BstYI RGATCY 2 cut(s) 874, 949
BsuI GTATCC 1 cut(s) 1371
BsuRI GGCC 2 cut(s) 51, 890
BtgI CCRYGG 2 cut(s) 213, 931
BtsCI GGATG 2 cut(s) 481, 662
BtsIMutI CAGTG 2 cut(s) 470, 885
Cac8I GCNNGC 3 cut(s) 49, 239, 681
CaiI CAGNNNCTG 1 cut(s) 1436
CfoI GCGC 3 cut(s) 155, 1361, 1664
Cfr13I GGNCC 1 cut(s) 1591
CseI GACGC 2 cut(s) 124, 329
Csp6I GTAC 8 cut(s) 229, 425, 512, 562, 646, 825, 1188, 1320
CviAII CATG 6 cut(s) 62, 630, 776, 932, 1019, 1547
CviQI GTAC 8 cut(s) 229, 425, 512, 562, 646, 825, 1188, 1320
DdeI CTNAG 5 cut(s) 220, 246, 487, 507, 726
DinI GGCGCC 1 cut(s) 1663
DpnI GATC 7 cut(s) 262, 387, 534, 876, 951, 1017, 1527
DpnII GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
DraI TTTAAA 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 931
Eco47I GGWCC 1 cut(s) 1591
Eco57I CTGAAG 3 cut(s) 548, 878, 1475
EcoO109I RGGNCCY 1 cut(s) 1591
EcoRI GAATTC 2 cut(s) 273, 818
EcoRII CCWGG 3 cut(s) 565, 958, 1665
EcoT14I CCWWGG 1 cut(s) 931
EcoT22I ATGCAT 1 cut(s) 1522
EgeI GGCGCC 1 cut(s) 1663
EheI GGCGCC 1 cut(s) 1663
ErhI CCWWGG 1 cut(s) 931
FaeI CATG 6 cut(s) 65, 633, 779, 935, 1022, 1550
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 1237
FatI CATG 6 cut(s) 61, 629, 775, 931, 1018, 1546
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 1 cut(s) 1525
Fnu4HI GCNGC 7 cut(s) 333, 492, 495, 578, 1109, 1247, 1601
FokI GGATG 2 cut(s) 488, 669
Fsp4HI GCNGC 7 cut(s) 333, 492, 495, 578, 1109, 1247, 1601
FspBI CTAG 5 cut(s) 590, 1013, 1347, 1382, 1595
GlaI GCGC 3 cut(s) 154, 1360, 1663
GluI GCNGC 7 cut(s) 333, 492, 495, 578, 1109, 1247, 1601
GsuI CTGGAG 2 cut(s) 593, 963
HaeII RGCGCY 2 cut(s) 1362, 1665
HaeIII GGCC 2 cut(s) 51, 890
HapII CCGG 1 cut(s) 641
HgaI GACGC 2 cut(s) 124, 329
HhaI GCGC 3 cut(s) 155, 1361, 1664
Hin1I GRCGYC 2 cut(s) 135, 1662
Hin1II CATG 6 cut(s) 65, 633, 779, 935, 1022, 1550
Hin6I GCGC 3 cut(s) 153, 1359, 1662
HinP1I GCGC 3 cut(s) 153, 1359, 1662
HincII GTYRAC 3 cut(s) 184, 1183, 1215
HindII GTYRAC 3 cut(s) 184, 1183, 1215
HinfI GANTC 8 cut(s) 89, 207, 218, 1094, 1286, 1371, 1426, 1433
HpaII CCGG 1 cut(s) 641
HphI GGTGA 3 cut(s) 46, 187, 1574
Hpy166II GTNNAC 8 cut(s) 130, 184, 195, 206, 217, 514, 1183, 1215
Hpy8I GTNNAC 8 cut(s) 130, 184, 195, 206, 217, 514, 1183, 1215
Hpy99I CGWCG 2 cut(s) 140, 237
HpyCH4III ACNGT 7 cut(s) 22, 233, 1064, 1180, 1187, 1541, 1562
HpyCH4IV ACGT 1 cut(s) 461
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 141, 150, 176, 503, 1246, 1397
HpyF3I CTNAG 5 cut(s) 220, 246, 487, 507, 726
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 2 cut(s) 135, 1662
Hsp92II CATG 6 cut(s) 65, 633, 779, 935, 1022, 1550
HspAI GCGC 3 cut(s) 153, 1359, 1662
KasI GGCGCC 1 cut(s) 1661
Ksp22I TGATCA 1 cut(s) 1525
Kzo9I GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 2 cut(s) 574, 1120
Lsp1109I GCAGC 7 cut(s) 344, 478, 481, 589, 1120, 1233, 1587
LweI GCATC 1 cut(s) 506
MaeI CTAG 5 cut(s) 590, 1013, 1347, 1382, 1595
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 5 cut(s) 401, 468, 1484, 1541, 1562
MalI GATC 7 cut(s) 262, 387, 534, 876, 951, 1017, 1527
MboI GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
MboII GAAGA 6 cut(s) 138, 431, 1001, 1079, 1185, 1266
MfeI CAATTG 2 cut(s) 501, 1466
MflI RGATCY 2 cut(s) 874, 949
MhlI GDGCHC 1 cut(s) 447
MlsI TGGCCA 1 cut(s) 890
MluNI TGGCCA 1 cut(s) 890
Mly113I GGCGCC 1 cut(s) 1662
MlyI GAGTC 4 cut(s) 201, 212, 1088, 1380
MmeI TCCRAC 2 cut(s) 882, 1035
Mox20I TGGCCA 1 cut(s) 890
Mph1103I ATGCAT 1 cut(s) 1522
MscI TGGCCA 1 cut(s) 890
MseI TTAA 7 cut(s) 408, 1235, 1449, 1496, 1536, 1568, 1638
Msp20I TGGCCA 1 cut(s) 890
MspCI CTTAAG 1 cut(s) 1567
MspI CCGG 1 cut(s) 641
MspR9I CCNGG 4 cut(s) 567, 641, 960, 1667
MunI CAATTG 2 cut(s) 501, 1466
Mva1269I GAATGC 1 cut(s) 1522
MvaI CCWGG 3 cut(s) 567, 960, 1667
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 7 cut(s) 44, 141, 150, 176, 503, 1246, 1397
NarI GGCGCC 1 cut(s) 1662
NciI CCSGG 1 cut(s) 641
NcoI CCATGG 1 cut(s) 931
NdeII GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
NlaIII CATG 6 cut(s) 65, 633, 779, 935, 1022, 1550
NlaIV GGNNCC 3 cut(s) 348, 771, 1663
NmuCI GTSAC 3 cut(s) 401, 468, 1562
NsiI ATGCAT 1 cut(s) 1522
PciSI GCTCTTC 1 cut(s) 151
PctI GAATGC 1 cut(s) 1522
PfeI GAWTC 4 cut(s) 89, 1286, 1426, 1433
PflMI CCANNNNNTGG 1 cut(s) 630
PfoI TCCNGGA 1 cut(s) 639
PkrI GCNGC 7 cut(s) 334, 493, 496, 579, 1110, 1248, 1602
PleI GAGTC 4 cut(s) 201, 212, 1088, 1379
PluTI GGCGCC 1 cut(s) 1665
PpsI GAGTC 4 cut(s) 201, 212, 1088, 1379
PpuMI RGGWCCY 1 cut(s) 1591
PshAI GACNNNNGTC 1 cut(s) 1062
Psp5II RGGWCCY 1 cut(s) 1591
Psp6I CCWGG 3 cut(s) 565, 958, 1665
PspGI CCWGG 3 cut(s) 565, 958, 1665
PspN4I GGNNCC 3 cut(s) 348, 771, 1663
PspPI GGNCC 1 cut(s) 1591
PspPPI RGGWCCY 1 cut(s) 1591
PstI CTGCAG 1 cut(s) 1251
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 2 cut(s) 874, 949
RsaI GTAC 8 cut(s) 230, 426, 513, 563, 647, 826, 1189, 1321
RsaNI GTAC 8 cut(s) 229, 425, 512, 562, 646, 825, 1188, 1320
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 7 cut(s) 408, 1235, 1449, 1496, 1536, 1568, 1638
SatI GCNGC 7 cut(s) 333, 492, 495, 578, 1109, 1247, 1601
Sau3AI GATC 7 cut(s) 260, 385, 532, 874, 949, 1015, 1525
Sau96I GGNCC 1 cut(s) 1591
ScaI AGTACT 1 cut(s) 426
SchI GAGTC 4 cut(s) 201, 212, 1088, 1380
ScrFI CCNGG 4 cut(s) 567, 641, 960, 1667
SduI GDGCHC 1 cut(s) 447
SfaNI GCATC 1 cut(s) 506
SfcI CTRYAG 2 cut(s) 414, 1247
SfoI GGCGCC 1 cut(s) 1663
SinI GGWCC 1 cut(s) 1591
SmlI CTYRAG 2 cut(s) 692, 1567
SmoI CTYRAG 2 cut(s) 692, 1567
SsiI CCGC 3 cut(s) 4, 151, 953
SspDI GGCGCC 1 cut(s) 1661
SspI AATATT 1 cut(s) 1314
SspMI CTAG 5 cut(s) 590, 1013, 1347, 1382, 1595
StyD4I CCNGG 4 cut(s) 565, 639, 958, 1665
StyI CCWWGG 1 cut(s) 931
TaaI ACNGT 7 cut(s) 22, 233, 1064, 1180, 1187, 1541, 1562
TaiI ACGT 1 cut(s) 464
TaqI TCGA 2 cut(s) 259, 1092
TatI WGTACW 4 cut(s) 424, 645, 824, 1187
TfiI GAWTC 4 cut(s) 89, 1286, 1426, 1433
Tru1I TTAA 7 cut(s) 408, 1235, 1449, 1496, 1536, 1568, 1638
Tru9I TTAA 7 cut(s) 408, 1235, 1449, 1496, 1536, 1568, 1638
TscAI CASTG 2 cut(s) 477, 885
TseFI GTSAC 3 cut(s) 401, 468, 1562
TseI GCWGC 7 cut(s) 332, 491, 494, 577, 1108, 1246, 1600
Tsp45I GTSAC 3 cut(s) 401, 468, 1562
TspDTI ATGAA 7 cut(s) 242, 422, 492, 599, 601, 1266, 1662
TspGWI ACGGA 2 cut(s) 960, 1341
TspRI CASTG 2 cut(s) 477, 885
Van91I CCANNNNNTGG 1 cut(s) 630
Vha464I CTTAAG 1 cut(s) 1567
VpaK11BI GGWCC 1 cut(s) 1591
XapI RAATTY 4 cut(s) 114, 273, 818, 1046
XbaI TCTAGA 1 cut(s) 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XspI CTAG 5 cut(s) 590, 1013, 1347, 1382, 1595
ZrmI AGTACT 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.