Prupe.2G047700_v2.0.a1

DNA-directed RNA polymerases I and III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0004203
Physical Location & Seq
Reverse (-)
5493829 .. 5495361
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G047700.1

Sequence Viewer

Length: 279 bp
ATGGAACACGGGTCCTTAACTGATCCCAGCCAATCTACATTTTCTTTGCTTTACAATCCAAGAACAAAATTTTGTGGGTACAGCATTCCTCATCCCTCGGATAACCGAGTTAATATCAGAATTCAGACAACAGGTGATGCAGCAAAAGATGTGTTCAACGATTCATGCCAGGATCTCATGGTGATTTGCCAACACGTGAGGAGCACTTTTGACAAGGCTGTCGTTGATTTTAGAATGAGCAAATCCGTAAATGGCATGGATATTGACTCAAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
RNA_pol_L_2

Protein Analysis

93

Amino Acids

10.32

Weight (kDa)

5.79

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AclWI GGATC 2 cut(s) 17, 180
AcsI RAATTY 2 cut(s) 68, 120
AcvI CACGTG 1 cut(s) 196
AfaI GTAC 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 1 cut(s) 157
AjnI CCWGG 1 cut(s) 168
Alw21I GWGCWC 1 cut(s) 206
AlwI GGATC 2 cut(s) 17, 180
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 68, 120
AspS9I GGNCC 1 cut(s) 12
AsuHPI GGTGA 2 cut(s) 146, 193
AvaII GGWCC 1 cut(s) 12
BbrPI CACGTG 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 152
BciT130I CCWGG 1 cut(s) 170
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 127
BsaAI YACGTR 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 2 cut(s) 22, 172
BspLI GGNNCC 1 cut(s) 13
BspPI GGATC 2 cut(s) 17, 180
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 22, 172
Bst2UI CCWGG 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 196
BstF5I GGATG 1 cut(s) 91
BstKTI GATC 2 cut(s) 25, 175
BstMBI GATC 2 cut(s) 22, 172
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BtsCI GGATG 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 79
CviAII CATG 3 cut(s) 165, 178, 256
CviJI RGCY 2 cut(s) 30, 218
CviKI_1 RGCY 2 cut(s) 30, 218
CviQI GTAC 1 cut(s) 79
DpnI GATC 2 cut(s) 24, 174
DpnII GATC 2 cut(s) 22, 172
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 12
Eco72I CACGTG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 168, 181, 259
FaiI YATR 3 cut(s) 166, 179, 257
FatI CATG 3 cut(s) 164, 177, 255
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
GsaI CCCAGC 1 cut(s) 30
Hin1II CATG 3 cut(s) 168, 181, 259
HinfI GANTC 2 cut(s) 161, 266
HphI GGTGA 2 cut(s) 146, 193
Hpy188I TCNGA 3 cut(s) 100, 119, 126
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 3 cut(s) 168, 181, 259
Kzo9I GATC 2 cut(s) 22, 172
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 4 cut(s) 40, 117, 155, 182
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 127
MaeII ACGT 1 cut(s) 195
MalI GATC 2 cut(s) 24, 174
MboI GATC 2 cut(s) 22, 172
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 3 cut(s) 68, 120, 274
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 3 cut(s) 99, 106, 192
MseI TTAA 2 cut(s) 17, 111
MspR9I CCNGG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 84
MvaI CCWGG 1 cut(s) 170
NdeII GATC 2 cut(s) 22, 172
NlaIII CATG 3 cut(s) 168, 181, 259
NlaIV GGNNCC 1 cut(s) 13
PctI GAATGC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 260
PmaCI CACGTG 1 cut(s) 196
PmlI CACGTG 1 cut(s) 196
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 168
PspCI CACGTG 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 1 cut(s) 12
PspPPI RGGWCCY 1 cut(s) 12
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SaqAI TTAA 2 cut(s) 17, 111
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 22, 172
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 1 cut(s) 206
SetI ASST 2 cut(s) 136, 198
SfaNI GCATC 1 cut(s) 127
SinI GGWCC 1 cut(s) 12
Sse9I AATT 3 cut(s) 68, 120, 274
StyD4I CCNGG 1 cut(s) 168
TaiI ACGT 1 cut(s) 198
TasI AATT 3 cut(s) 68, 120, 274
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 17, 111
Tru9I TTAA 2 cut(s) 17, 111
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 153
TspGWI ACGGA 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 2 cut(s) 68, 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.