Rorug02G0281300

DNA-directed RNA polymerases I and III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004203
Physical Location & Seq
Forward (+)
30360067 .. 30360701
635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0281300.1

Sequence Viewer

Length: 465 bp
ATGTTGTTTGAGCTGACCTTCAACAACATAATGACCATGGTGGCGGGGAAGAGGTATGTGGGGGACTGTGTTGCGAACAAGGAAGAGGGGAAGGAGTTCAGTGAGATCATGGATGAGGCTTTTTCGTACGGTGGGGGAACCAACCCCGGAGAATTCATGCCCTTTTTGAGATGGTTTGGCGGTAATGGTTATGAGAGGAAGTTGAAGAAGTTGGGCTGGAGAGCAGATTTGTTCTTGCAGCGTCTAATTGATGAGCACCGGAACAAGAGTGCTTCGGAGAGTAAAAATACCATGATCGACCATTTGCTTTCTCAGCAGGAGTCACAACCTGAGTATTACACTGATGAGATTATCAATGGACTTATACTGATTCTATTATTGGCTGGCACTGATACATCGGCAGTGACACTAGAATGGGCTATGTCTAATTTGCTAAATGCATCCAGATGCATTAGAGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
RNA_pol_L_2

Protein Analysis

154

Amino Acids

17.5

Weight (kDa)

4.98

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 146 6.6e-15 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 44, 180
AcsI RAATTY 1 cut(s) 152
AfaI GTAC 1 cut(s) 128
AgsI TTSAA 2 cut(s) 22, 205
AluBI AGCT 1 cut(s) 13
AluI AGCT 1 cut(s) 13
Alw21I GWGCWC 1 cut(s) 258
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 95
AsuC2I CCSGG 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 250
BccI CCATC 1 cut(s) 165
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 2 cut(s) 410, 463
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 437, 449
BpmI CTGGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 36, 145
BsaWI WCCGGW 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 36, 145
BseGI GGATG 2 cut(s) 118, 440
BseMII CTCAG 2 cut(s) 321, 326
BseXI GCAGC 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 147, 259
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 2 cut(s) 105, 294
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 2 cut(s) 44, 180
BspCNI CTCAG 2 cut(s) 322, 325
BspLI GGNNCC 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 36, 145
BssMI GATC 2 cut(s) 105, 294
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 68, 131
Bst6I CTCTTC 2 cut(s) 44, 78
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 2 cut(s) 312, 330
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 118, 440
BstKTI GATC 2 cut(s) 108, 297
BstMBI GATC 2 cut(s) 105, 294
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 118, 440
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 4 cut(s) 106, 339, 387, 408
Cac8I GCNNGC 1 cut(s) 385
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 127
CviAII CATG 4 cut(s) 37, 109, 157, 292
CviJI RGCY 6 cut(s) 13, 119, 216, 383, 419, 462
CviKI_1 RGCY 6 cut(s) 13, 119, 216, 383, 419, 462
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 2 cut(s) 312, 330
DpnI GATC 2 cut(s) 107, 296
DpnII GATC 2 cut(s) 105, 294
Eam1104I CTCTTC 2 cut(s) 44, 78
EarI CTCTTC 2 cut(s) 44, 78
Eco130I CCWWGG 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 36
EcoT22I ATGCAT 2 cut(s) 442, 452
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 4 cut(s) 40, 112, 160, 295
FaiI YATR 9 cut(s) 29, 38, 57, 110, 158, 192, 293, 365, 422
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 36, 108, 156, 291
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 2 cut(s) 125, 427
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 2 cut(s) 410, 463
GluI GCNGC 1 cut(s) 239
GsuI CTGGAG 1 cut(s) 238
HapII CCGG 2 cut(s) 147, 259
HgaI GACGC 1 cut(s) 230
Hin1II CATG 4 cut(s) 40, 112, 160, 295
HinfI GANTC 2 cut(s) 320, 370
HpaII CCGG 2 cut(s) 147, 259
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 1 cut(s) 444
HpyAV CCTTC 3 cut(s) 28, 85, 452
HpyCH4III ACNGT 2 cut(s) 68, 131
HpyCH4V TGCA 3 cut(s) 238, 440, 450
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 2 cut(s) 312, 330
Hsp92II CATG 4 cut(s) 40, 112, 160, 295
Kzo9I GATC 2 cut(s) 105, 294
LpnPI CCDG 7 cut(s) 160, 202, 272, 302, 342, 369, 457
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 2 cut(s) 437, 449
MaeI CTAG 2 cut(s) 410, 463
MaeIII GTNAC 2 cut(s) 321, 403
MalI GATC 2 cut(s) 107, 296
MboI GATC 2 cut(s) 105, 294
MboII GAAGA 3 cut(s) 61, 95, 217
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 3 cut(s) 152, 246, 427
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 4 cut(s) 45, 79, 109, 189
Mph1103I ATGCAT 2 cut(s) 442, 452
MroXI GAANNNNTTC 1 cut(s) 95
MslI CAYNNNNRTG 2 cut(s) 412, 445
MspI CCGG 2 cut(s) 147, 259
MspR9I CCNGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 313
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 36
NdeII GATC 2 cut(s) 105, 294
NlaIII CATG 4 cut(s) 40, 112, 160, 295
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 2 cut(s) 321, 403
NsiI ATGCAT 2 cut(s) 442, 452
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 370
Pfl23II CGTACG 1 cut(s) 126
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 139
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 2 cut(s) 412, 445
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 2 cut(s) 105, 294
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 258
SetI ASST 4 cut(s) 15, 20, 56, 331
SfaNI GCATC 2 cut(s) 437, 449
SmiMI CAYNNNNRTG 2 cut(s) 412, 445
Sse9I AATT 3 cut(s) 152, 246, 427
SsiI CCGC 2 cut(s) 44, 180
SspMI CTAG 2 cut(s) 410, 463
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 68, 131
TaqI TCGA 1 cut(s) 297
TasI AATT 3 cut(s) 152, 246, 427
TfiI GAWTC 1 cut(s) 370
TscAI CASTG 4 cut(s) 106, 346, 394, 408
TseFI GTSAC 2 cut(s) 321, 403
TseI GCWGC 1 cut(s) 238
Tsp45I GTSAC 2 cut(s) 321, 403
TspDTI ATGAA 1 cut(s) 145
TspRI CASTG 4 cut(s) 106, 346, 394, 408
XapI RAATTY 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 2 cut(s) 410, 463
Zsp2I ATGCAT 2 cut(s) 442, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.