Rh1AG141500

DNA-directed RNA polymerases I and III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004203
Physical Location & Seq
Forward (+)
26752662 .. 26757637
4976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG141500.1

Sequence Viewer

Length: 369 bp
ATGTCGAAGAAGAGTCCTTTGTGCACAAATATTTCAGAAAAATTGGAAGAATCGGGTATTGAGGGTACCGAGAATCAAATTCGGGCAGGGTTTTGTAAAGAAATGGAACTTGGGTCCCTTTCTGATCCCACCCAATCCTCATTCTCTTTAGTTGATGAGGATCATACATTCGCAAATGCTGTCAGGTTTACTTTGAATCAGGATCCAAGGACGAAATTCTGTGGAATCCAGACTACAGGTGATCCAGCAAACGATGTATTCAAAGATGCATGCCAGGATCTCATGGTGATGTGCCAGCATGTGAGCAACACTTTTAACAAGGCTGTGGTTGATTTCAAATTGAGCAATTCTGTGGAGGGAGAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
RNA_pol_L_2

Protein Analysis

122

Amino Acids

13.45

Weight (kDa)

4.61

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_L_2 PF13656 46 - 103 6e-09 RNA polymerase Rpb3/Rpb11 dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 367
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 65
AclWI GGATC 6 cut(s) 119, 168, 197, 210, 236, 285
AcsI RAATTY 2 cut(s) 78, 215
AfaI GTAC 1 cut(s) 67
AgsI TTSAA 3 cut(s) 196, 262, 337
AjnI CCWGG 1 cut(s) 273
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
Alw21I GWGCWC 1 cut(s) 26
Alw44I GTGCAC 1 cut(s) 22
AlwI GGATC 6 cut(s) 119, 168, 197, 210, 236, 285
ApaLI GTGCAC 1 cut(s) 22
ApoI RAATTY 2 cut(s) 78, 215
Asp718I GGTACC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 251, 298
AvaII GGWCC 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 26
BamHI GGATCC 1 cut(s) 202
BanI GGYRCC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 4 cut(s) 67, 115, 116, 204
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 5 cut(s) 124, 160, 202, 241, 277
BspLI GGNNCC 4 cut(s) 67, 115, 116, 204
BspPI GGATC 6 cut(s) 119, 168, 197, 210, 236, 285
BspT107I GGYRCC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 5 cut(s) 124, 160, 202, 241, 277
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 271, 296
BstKTI GATC 5 cut(s) 127, 163, 205, 244, 280
BstMBI GATC 5 cut(s) 124, 160, 202, 241, 277
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 2 cut(s) 273, 302
BstSCI CCNGG 1 cut(s) 273
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 26
BstX2I RGATCY 2 cut(s) 202, 277
BstYI RGATCY 2 cut(s) 202, 277
Cac8I GCNNGC 2 cut(s) 271, 296
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 66
CviAII CATG 3 cut(s) 270, 283, 299
CviJI RGCY 1 cut(s) 323
CviKI_1 RGCY 1 cut(s) 323
CviQI GTAC 1 cut(s) 66
DpnI GATC 5 cut(s) 126, 162, 204, 243, 279
DpnII GATC 5 cut(s) 124, 160, 202, 241, 277
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco130I CCWWGG 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 3 cut(s) 273, 286, 302
FaiI YATR 5 cut(s) 165, 271, 284, 300, 367
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 269, 282, 298
Hin1II CATG 3 cut(s) 273, 286, 302
HinfI GANTC 5 cut(s) 13, 50, 73, 196, 225
HphI GGTGA 2 cut(s) 251, 298
Hpy166II GTNNAC 2 cut(s) 24, 189
Hpy188I TCNGA 2 cut(s) 37, 124
Hpy188III TCNNGA 2 cut(s) 200, 229
Hpy8I GTNNAC 2 cut(s) 24, 189
HpyCH4V TGCA 2 cut(s) 24, 269
Hsp92II CATG 3 cut(s) 273, 286, 302
KflI GGGWCCC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 69
Kzo9I GATC 5 cut(s) 124, 160, 202, 241, 277
LpnPI CCDG 9 cut(s) 72, 169, 185, 222, 242, 258, 260, 287, 308
LweI GCATC 1 cut(s) 256
MalI GATC 5 cut(s) 126, 162, 204, 243, 279
MboI GATC 5 cut(s) 124, 160, 202, 241, 277
MboII GAAGA 3 cut(s) 19, 22, 59
MflI RGATCY 2 cut(s) 202, 277
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 5 cut(s) 41, 78, 215, 338, 346
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 4 cut(s) 55, 148, 151, 349
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
NdeII GATC 5 cut(s) 124, 160, 202, 241, 277
NlaIII CATG 3 cut(s) 273, 286, 302
NlaIV GGNNCC 4 cut(s) 67, 115, 116, 204
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 2 cut(s) 273, 302
PaeI GCATGC 1 cut(s) 273
PfeI GAWTC 4 cut(s) 50, 73, 196, 225
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
PpuMI RGGWCCY 1 cut(s) 114
PsiI TTATAA 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 4 cut(s) 67, 115, 116, 204
PspPI GGNCC 1 cut(s) 114
PspPPI RGGWCCY 1 cut(s) 114
PsuI RGATCY 2 cut(s) 202, 277
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 287
SaqAI TTAA 1 cut(s) 315
Sau3AI GATC 5 cut(s) 124, 160, 202, 241, 277
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 26
SetI ASST 2 cut(s) 188, 241
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 287
SphI GCATGC 1 cut(s) 273
Sse9I AATT 5 cut(s) 41, 78, 215, 338, 346
SspI AATATT 1 cut(s) 31
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 1 cut(s) 206
TaqI TCGA 1 cut(s) 5
TasI AATT 5 cut(s) 41, 78, 215, 338, 346
TfiI GAWTC 4 cut(s) 50, 73, 196, 225
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
VneI GTGCAC 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 2 cut(s) 78, 215
XceI RCATGY 2 cut(s) 273, 302
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.