Prupe.2G050300_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0009420
Physical Location & Seq
Forward (+)
5832216 .. 5836205
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G050300.1

Sequence Viewer

Length: 774 bp
ATGAATTTGGGCGACTGCAAGGTCAATCCATTGGGTCGTAATGGGGGCATGAACAAGGACAAGCCCTTTAAAATGTTTGTGGGTTATGATCAACGTGAAGACATTGCATATGAGGTTTGTCGACACTCAATATTGAAGAGATCTTCAATCCCAGTTGAGATCATACCACTTAAACAATCTGATCTGAGAAAGGAAGGCCTATATTGGCGTGAAAGAGGCCAATTGGAGAGCACAGAGTTTTCTTTTACTCGGTTCTTGACTCCATATTTGGCCAACTATGAAGGTTGGGCAGTGTTTGTGGATTGTGATTTTCTTTACCTAGCTGACATTAAGGAGCTAAGAGATTTGGTTGATGAGAAATATGCCATCATGTGTGTTCAACATGATTACACCCCAAAGGAGACAACAAAAATGGATGGAGCATTGCAAACTGTGTACCCTAGGAAGAATTGGTCTTCCATGGTGTTGTACAATTGTGAACATCCCAAAAACCGGGTCTTGACGCCGGAGGTTGTGAACACACAAACCGGCGCTTTTCTTCATAGGTTTCAGTGGCTTGAGGACGAAGAAATTGGGTCCATCCCATTTGTTTGGAATTTTCTTGAGGGACATAACCAAGTTGTTGAAGGTAATCCCCGGACTTTCCCTAAGGCAATACACTATACTCGGGGAGGACCGTGGTTTGAGGCTTGGAAGAATTGCGAGTTTGCAGACCTGTGGTTGAATGAAATGGAGACGTACCTGCAGGAAACGAAGAAGAAAGTCGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

30.29

Weight (kDa)

5.62

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 750
AccI GTMKAC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 2 cut(s) 4, 595
AcyI GRCGYC 1 cut(s) 503
AfaI GTAC 3 cut(s) 437, 470, 740
AfiI CCNNNNNNNGG 1 cut(s) 492
AgsI TTSAA 5 cut(s) 136, 147, 380, 626, 724
AluBI AGCT 2 cut(s) 323, 337
AluI AGCT 2 cut(s) 323, 337
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 2 cut(s) 395, 728
Ama87I CYCGRG 1 cut(s) 666
AoxI GGCC 3 cut(s) 196, 217, 270
ApoI RAATTY 2 cut(s) 4, 595
ArsI GACNNNNNNTTYG 4 cut(s) 250, 282, 480, 512
AspA2I CCTAGG 1 cut(s) 440
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 2 cut(s) 576, 674
AsuC2I CCSGG 2 cut(s) 494, 637
AvaI CYCGRG 1 cut(s) 666
AvaII GGWCC 2 cut(s) 576, 674
AvrII CCTAGG 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 648
BalI TGGCCA 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 105, 447
Bbv12I GWGCWC 1 cut(s) 233
BccI CCATC 3 cut(s) 374, 410, 587
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 494, 637
BcoDI GTCTC 2 cut(s) 395, 728
BfaI CTAG 2 cut(s) 320, 441
BfmI CTRYAG 1 cut(s) 743
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 750
BglII AGATCT 1 cut(s) 140
BlnI CCTAGG 1 cut(s) 440
Bme1390I CCNGG 2 cut(s) 494, 637
Bme18I GGWCC 2 cut(s) 576, 674
BmeT110I CYCGRG 1 cut(s) 666
BmgT120I GGNCC 2 cut(s) 576, 674
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 2 cut(s) 494, 637
BmrI ACTGGG 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 2 cut(s) 105, 447
BpuEI CTTGAG 2 cut(s) 578, 623
BpuMI CCSGG 2 cut(s) 494, 637
BsaHI GRCGYC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 440, 459, 635, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 152
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 102, 422
BseDI CCNNGG 4 cut(s) 440, 459, 635, 677
BseGI GGATG 3 cut(s) 421, 481, 579
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMI GCAATG 2 cut(s) 102, 422
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 152
BshFI GGCC 3 cut(s) 198, 219, 272
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 666
BsiSI CCGG 4 cut(s) 493, 506, 528, 637
BslFI GGGAC 1 cut(s) 621
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 2 cut(s) 395, 728
BsmBI CGTCTC 1 cut(s) 728
BsmFI GGGAC 1 cut(s) 621
BsnI GGCC 3 cut(s) 198, 219, 272
BsoBI CYCGRG 1 cut(s) 666
Bsp1286I GDGCHC 1 cut(s) 233
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 4 cut(s) 88, 140, 159, 181
Bsp19I CCATGG 1 cut(s) 459
BspANI GGCC 3 cut(s) 198, 219, 272
BspCNI CTCAG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 577
BspMAI CTGCAG 1 cut(s) 747
BspMI ACCTGC 1 cut(s) 750
BsrDI GCAATG 2 cut(s) 102, 422
BsrFI RCCGGY 1 cut(s) 527
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 4 cut(s) 440, 459, 635, 677
BssMI GATC 4 cut(s) 88, 140, 159, 181
BssNI GRCGYC 1 cut(s) 503
BssT1I CCWWGG 2 cut(s) 440, 459
Bst4CI ACNGT 2 cut(s) 433, 678
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 468
BstDEI CTNAG 3 cut(s) 185, 338, 648
BstDSI CCRYGG 2 cut(s) 459, 677
BstF5I GGATG 3 cut(s) 421, 481, 579
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 4 cut(s) 91, 143, 162, 184
BstMAI GTCTC 2 cut(s) 395, 728
BstMBI GATC 4 cut(s) 88, 140, 159, 181
BstSCI CCNGG 2 cut(s) 492, 635
BstSFI CTRYAG 1 cut(s) 743
BstV2I GAAGAC 2 cut(s) 105, 447
BstX2I RGATCY 1 cut(s) 140
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 648
BsuRI GGCC 3 cut(s) 198, 219, 272
BtgI CCRYGG 2 cut(s) 459, 677
BtsCI GGATG 3 cut(s) 421, 481, 579
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 557
BveI ACCTGC 1 cut(s) 750
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 576, 674
CseI GACGC 1 cut(s) 511
Csp6I GTAC 3 cut(s) 436, 469, 739
CviAII CATG 4 cut(s) 49, 370, 383, 460
CviJI RGCY 8 cut(s) 64, 198, 219, 272, 323, 337, 556, 689
CviKI_1 RGCY 8 cut(s) 64, 198, 219, 272, 323, 337, 556, 689
CviQI GTAC 3 cut(s) 436, 469, 739
DdeI CTNAG 3 cut(s) 185, 338, 648
DpnI GATC 4 cut(s) 90, 142, 161, 183
DpnII GATC 4 cut(s) 88, 140, 159, 181
DraI TTTAAA 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 440, 459
Eco147I AGGCCT 1 cut(s) 198
Eco47I GGWCC 2 cut(s) 576, 674
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 666
EcoT14I CCWWGG 2 cut(s) 440, 459
ErhI CCWWGG 2 cut(s) 440, 459
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 4 cut(s) 52, 373, 386, 463
FaqI GGGAC 1 cut(s) 621
FatI CATG 4 cut(s) 48, 369, 382, 459
FauNDI CATATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 121
FokI GGATG 3 cut(s) 428, 468, 566
FspBI CTAG 2 cut(s) 320, 441
GlaI GCGC 1 cut(s) 532
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 3 cut(s) 198, 219, 272
HapII CCGG 4 cut(s) 493, 506, 528, 637
HgaI GACGC 1 cut(s) 511
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 1 cut(s) 503
Hin1II CATG 4 cut(s) 52, 373, 386, 463
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 1 cut(s) 122
HindII GTYRAC 1 cut(s) 122
HinfI GANTC 1 cut(s) 259
HpaII CCGG 4 cut(s) 493, 506, 528, 637
Hpy166II GTNNAC 4 cut(s) 122, 436, 479, 517
Hpy188I TCNGA 2 cut(s) 181, 186
Hpy188III TCNNGA 3 cut(s) 256, 499, 602
Hpy8I GTNNAC 4 cut(s) 122, 436, 479, 517
HpyAV CCTTC 3 cut(s) 188, 275, 620
HpyCH4III ACNGT 2 cut(s) 433, 678
HpyCH4IV ACGT 2 cut(s) 94, 737
HpyCH4V TGCA 5 cut(s) 18, 107, 427, 710, 745
HpyF3I CTNAG 3 cut(s) 185, 338, 648
HpySE526I ACGT 2 cut(s) 94, 737
Hsp92I GRCGYC 1 cut(s) 503
Hsp92II CATG 4 cut(s) 52, 373, 386, 463
HspAI GCGC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 4 cut(s) 88, 140, 159, 181
LmnI GCTCC 2 cut(s) 334, 419
LpnPI CCDG 8 cut(s) 165, 506, 519, 541, 650, 728, 731, 755
MaeI CTAG 2 cut(s) 320, 441
MaeII ACGT 2 cut(s) 94, 737
MalI GATC 4 cut(s) 90, 142, 161, 183
MboI GATC 4 cut(s) 88, 140, 159, 181
MfeI CAATTG 2 cut(s) 221, 472
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 8 cut(s) 4, 221, 448, 472, 570, 595, 697, 769
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 7 cut(s) 106, 209, 502, 553, 598, 665, 679
Mox20I TGGCCA 1 cut(s) 272
MscI TGGCCA 1 cut(s) 272
MseI TTAA 3 cut(s) 69, 171, 330
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 4 cut(s) 493, 506, 528, 637
MspR9I CCNGG 2 cut(s) 494, 637
MunI CAATTG 2 cut(s) 221, 472
NciI CCSGG 2 cut(s) 494, 637
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 109
NdeII GATC 4 cut(s) 88, 140, 159, 181
NlaIII CATG 4 cut(s) 52, 373, 386, 463
NlaIV GGNNCC 1 cut(s) 577
PceI AGGCCT 1 cut(s) 198
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 2 cut(s) 576, 674
PstI CTGCAG 1 cut(s) 747
PsuI RGATCY 1 cut(s) 140
RsaI GTAC 3 cut(s) 437, 470, 740
RsaNI GTAC 3 cut(s) 436, 469, 739
SalI GTCGAC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 69, 171, 330
Sau3AI GATC 4 cut(s) 88, 140, 159, 181
Sau96I GGNCC 2 cut(s) 576, 674
SbfI CCTGCAGG 1 cut(s) 747
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 494, 637
SdaI CCTGCAGG 1 cut(s) 747
SduI GDGCHC 1 cut(s) 233
SfcI CTRYAG 1 cut(s) 743
SinI GGWCC 2 cut(s) 576, 674
SmlI CTYRAG 2 cut(s) 557, 602
SmoI CTYRAG 2 cut(s) 557, 602
Sse8387I CCTGCAGG 1 cut(s) 747
Sse9I AATT 8 cut(s) 4, 221, 448, 472, 570, 595, 697, 769
SseBI AGGCCT 1 cut(s) 198
SspI AATATT 1 cut(s) 132
SspMI CTAG 2 cut(s) 320, 441
StuI AGGCCT 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 492, 635
StyI CCWWGG 2 cut(s) 440, 459
TaaI ACNGT 2 cut(s) 433, 678
TaiI ACGT 2 cut(s) 97, 740
TaqI TCGA 2 cut(s) 121, 765
TasI AATT 8 cut(s) 4, 221, 448, 472, 570, 595, 697, 769
TatI WGTACW 1 cut(s) 468
Tru1I TTAA 3 cut(s) 69, 171, 330
Tru9I TTAA 3 cut(s) 69, 171, 330
TscAI CASTG 2 cut(s) 297, 557
TspDTI ATGAA 5 cut(s) 17, 65, 294, 530, 741
TspRI CASTG 2 cut(s) 297, 557
VpaK11BI GGWCC 2 cut(s) 576, 674
XapI RAATTY 2 cut(s) 4, 595
XmaJI CCTAGG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 121
XspI CTAG 2 cut(s) 320, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.