Prupe.2G060300_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016381
Physical Location & Seq
Reverse (-)
7621406 .. 7626963
5558 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G060300.1

Sequence Viewer

Length: 1776 bp
ATGGCTGTCTCAGCCTTCAAATCGTCAAGCAAAAGGAGCAATGCCGCCACCAATTCCTCAGCATCAACAACCTCATCTGTTGGCAGACAGCAAATCACAGACAAAGATGATAACAACCCAGCAAAGAAAGCCCCGCTCAGGAGGTCAAGAAGCGTCAGCGCTTTCTCAAGGACCAGCCTTGACGCCTCCGCTGAAAGCTTCTTGAACAAGAGAGACAACCCTCTCTTCTGGTCAACGGACGCCCAAGCCGTTGACTCCCAAAAGCTAGTCAAACCCACCAAGCTTGACGCTGCAACATCGAAAGATTCGAGACCGGCGACCTCCGCTGGTGCTGATTCCAGAAGGGGCCGCTCCGGGTTGCGGAATTCCGATGCTTTGGGGACGAGGAAGGAGGCGGGTCGGAGCTTGTCGCGGGTCGACCCGAGCCGTCGGAACCGCTCCATTTCCCGCGGTCCGGATTCCAGGAGGCATTTCGTCAATTCTAAGAGTGACGATGAACAAGTATGGGGTTCGTTGAATAAAGTGGGCAGTGATGGGAAAAAGGGTGCTTTAGTTAGAAGCAGTTCTGATGAGTTGGATGAGTTGAAAGGTCTGCGTACATGGTCCAGCCAGCATTCACAAGATTCTGATGCAAATTTGTCTTGTTTGCAATCTACAAATTGGGAAGATGGAGTTTCAGTCGCAGGTTCATTATCTGGAGCAGAAGAGAAAACTGTTAAAGCAGTGTGTGAACGGATGAATATGGATCTTGGGCATTCTCATTTGTCAGTTCAAGGGAAACATTTGGAAGATGATAACCCGAGTACTAGTATATATGAAACAGTTCGATCTGAAGTGAGGCGTGCTATCTCTGAGATTCAAAATGACCTTGAAAGTGCTATGCGAAGGAGTAATGCTCCAGCACTTGCAACTACCGACATTGCTGATATCCCTCCTGACCTGGTAAATCCAAGTGCAGTTGAATTAGTTTTGGACATCAGAAGAGAATATGCCAAAAAGCTTGAGCAGTCCCGGGAACGCTCTAGAAAACTTCAATCGGACCTGGCTGTTGAGGAGCACCGTGGACAAGAGCTCAGCAGGATCCTGAAGGAAGTGCTTCCAGATCCCAAGACCTCTAATGTGCCAAAATCACGTCCGGGAAGAAAAGCTAGCATTGAAAGAAGGAAGATGTCAAAACGTCTAACAGAAGAAGCCATGTCTTATTTTGATGAGTGTGTATCCCTATCAACTTTTGATAGTTCTGACTTCTCATCGCCAGAAGACCCACCGCTCAACTTAGTTGGCTTTACTACTCCTGTTGGTAAAAGCATGTCTTTACTCCAAGCAAGTTCAACTGCTGCAGTCACCAATAACTCAAACTCTTGCCACAATGATAAACAGGGATTAATCATTGAAAATCAGTATACCAATGACCGTCATGCTTCAGGACTGATGGCAAGCAGTGGTCCTAGGGAGCCACAGATTAGTCCTAACAGTGCCAACATGCCCCAGGATTGGAAGTTCTCATTTGCTCATAAGCCAACAGAAACCCTTGAGCTTCAACCAGACATTATGAAGTACGCAAGACATTTTGAGAAAGGAATTGAGAAAGATGACACGGAATTGCAGATTGTAAGATCAAACTACTATGATTTGGGTGAGTACAATTTGCAGGCTGCAGCACAAAGCTATATGTTCGACAGGGTATTGTATAGAAATAGAATAGAGTCGGGTGGTATTTTACTCTGTGGCAATGGTATTTCAGGTCCTTTGTCACATTTTGCTTCTTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

592

Amino Acids

65.11

Weight (kDa)

8.58

Isoelectric Point (pI)

66.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016381)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04960 FvH4_7g05350
malus_domestica MD02G1265200.v1.1
prunus_persica Prupe.2G060300_v2.0.a1
pyrus_communis pycom02g22660
rosa_chinensis RchiOBHm_Chr1g0331161
rosa_laevigata RLG00000029749
rosa_multiflora Rmu_sc0006123.1_g000009
rosa_roxburghii Rroxscaffold_4G00319310
rosa_rugosa Rorug01G0091800
rosa_samantha Rh1AG114800 Rh1BG087500 Rh1CG110800 Rh1DG119500
rosa_wichuraiana Rw1G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 674
AccBSI CCGCTC 4 cut(s) 136, 351, 438, 1270
AccI GTMKAC 2 cut(s) 417, 1403
AccII CGCG 2 cut(s) 412, 450
AccIII TCCGGA 1 cut(s) 454
AclWI GGATC 4 cut(s) 753, 1075, 1088, 1097
AcsI RAATTY 2 cut(s) 364, 634
AcuI CTGAAG 3 cut(s) 852, 1106, 1407
AcyI GRCGYC 2 cut(s) 183, 240
AfaI GTAC 4 cut(s) 598, 805, 1559, 1643
AfeI AGCGCT 1 cut(s) 160
AfiI CCNNNNNNNGG 4 cut(s) 138, 360, 454, 1494
AhlI ACTAGT 1 cut(s) 806
AjiI CACGTC 1 cut(s) 1133
AjnI CCWGG 4 cut(s) 461, 939, 1041, 1488
AloI GAACNNNNNNTCC 2 cut(s) 1484, 1516
AluBI AGCT 9 cut(s) 198, 265, 283, 405, 1000, 1072, 1148, 1537, 1668
AluI AGCT 9 cut(s) 198, 265, 283, 405, 1000, 1072, 1148, 1537, 1668
Alw21I GWGCWC 2 cut(s) 1059, 1074
Alw26I GTCTC 3 cut(s) 13, 207, 304
AlwI GGATC 4 cut(s) 753, 1075, 1088, 1097
Ama87I CYCGRG 3 cut(s) 421, 799, 1011
Aor13HI TCCGGA 1 cut(s) 454
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 4 cut(s) 290, 1337, 1655, 1658
ApoI RAATTY 2 cut(s) 364, 634
AseI ATTAAT 1 cut(s) 1385
Asp700I GAANNNNTTC 3 cut(s) 562, 822, 1095
AspA2I CCTAGG 1 cut(s) 1448
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 7 cut(s) 171, 346, 452, 603, 1039, 1445, 1745
AsuC2I CCSGG 4 cut(s) 355, 1012, 1013, 1137
AsuHPI GGTGA 2 cut(s) 1336, 1649
AsuNHI GCTAGC 1 cut(s) 1148
AvaI CYCGRG 3 cut(s) 421, 799, 1011
AvaII GGWCC 6 cut(s) 171, 452, 603, 1039, 1445, 1745
AvrII CCTAGG 1 cut(s) 1448
BamHI GGATCC 1 cut(s) 1080
BanII GRGCYC 1 cut(s) 1074
BbsI GAAGAC 1 cut(s) 1266
Bbv12I GWGCWC 2 cut(s) 1059, 1074
BbvCI CCTCAGC 1 cut(s) 58
BbvI GCAGC 4 cut(s) 277, 1324, 1642, 1670
BccI CCATC 3 cut(s) 527, 662, 1426
BceAI ACGGC 2 cut(s) 233, 411
BciT130I CCWGG 4 cut(s) 463, 941, 1043, 1490
BciVI GTATCC 1 cut(s) 1228
BcnI CCSGG 4 cut(s) 355, 1012, 1013, 1137
BcoDI GTCTC 3 cut(s) 13, 207, 304
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 5 cut(s) 266, 807, 1023, 1149, 1449
BfmI CTRYAG 2 cut(s) 1338, 1656
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 674
BfuI GTATCC 1 cut(s) 1228
BisI GCNGC 6 cut(s) 45, 291, 349, 1338, 1656, 1659
BlnI CCTAGG 1 cut(s) 1448
BlpI GCTNAGC 1 cut(s) 1073
BlsI GCNGC 6 cut(s) 46, 292, 350, 1339, 1657, 1660
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 8 cut(s) 355, 463, 941, 1012, 1013, 1043, 1137, 1490
Bme18I GGWCC 6 cut(s) 171, 452, 603, 1039, 1445, 1745
BmeT110I CYCGRG 3 cut(s) 421, 799, 1011
BmgBI CACGTC 1 cut(s) 1133
BmgT120I GGNCC 7 cut(s) 171, 346, 452, 603, 1039, 1445, 1745
BmiI GGNNCC 4 cut(s) 347, 434, 1082, 1455
BmrFI CCNGG 8 cut(s) 355, 463, 941, 1012, 1013, 1043, 1137, 1490
BmsI GCATC 3 cut(s) 71, 361, 619
BmtI GCTAGC 1 cut(s) 1152
BpiI GAAGAC 1 cut(s) 1266
BplI GAGNNNNNCTC 2 cut(s) 880, 912
BpmI CTGGAG 2 cut(s) 717, 882
Bpu10I CCTNAGC 2 cut(s) 58, 137
Bpu1102I GCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 3 cut(s) 151, 1022, 1553
BpuMI CCSGG 4 cut(s) 355, 1012, 1013, 1137
BsaHI GRCGYC 2 cut(s) 183, 240
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 5 cut(s) 448, 1011, 1060, 1448, 1488
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 360, 454, 1494
Bse118I RCCGGY 1 cut(s) 313
Bse3DI GCAATG 3 cut(s) 46, 918, 1738
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 4 cut(s) 463, 941, 1043, 1490
BseDI CCNNGG 5 cut(s) 448, 1011, 1060, 1448, 1488
BseGI GGATG 2 cut(s) 583, 741
BseLI CCNNNNNNNGG 4 cut(s) 138, 360, 454, 1494
BseMI GCAATG 3 cut(s) 46, 918, 1738
BseMII CTCAG 5 cut(s) 24, 72, 151, 843, 1087
BseRI GAGGAG 1 cut(s) 1067
BseXI GCAGC 4 cut(s) 277, 1324, 1642, 1670
BseYI CCCAGC 1 cut(s) 118
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 2 cut(s) 412, 450
BshFI GGCC 1 cut(s) 348
BsiHKAI GWGCWC 2 cut(s) 1059, 1074
BsiHKCI CYCGRG 3 cut(s) 421, 799, 1011
BsiSI CCGG 5 cut(s) 314, 354, 455, 1012, 1136
BslFI GGGAC 2 cut(s) 394, 994
BslI CCNNNNNNNGG 4 cut(s) 138, 360, 454, 1494
BsmAI GTCTC 3 cut(s) 13, 207, 304
BsmFI GGGAC 2 cut(s) 394, 994
BsmI GAATGC 2 cut(s) 613, 754
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 304
BsoBI CYCGRG 3 cut(s) 421, 799, 1011
Bsp1286I GDGCHC 2 cut(s) 1059, 1074
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 5 cut(s) 745, 827, 1080, 1102, 1616
Bsp1720I GCTNAGC 1 cut(s) 1073
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 5 cut(s) 23, 71, 150, 844, 1086
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 2 cut(s) 412, 450
BspLI GGNNCC 4 cut(s) 347, 434, 1082, 1455
BspMAI CTGCAG 2 cut(s) 1342, 1660
BspMI ACCTGC 1 cut(s) 674
BspOI GCTAGC 1 cut(s) 1152
BspPI GGATC 4 cut(s) 753, 1075, 1088, 1097
BspTNI GGTCTC 1 cut(s) 304
BsrBI CCGCTC 4 cut(s) 136, 351, 438, 1270
BsrDI GCAATG 3 cut(s) 46, 918, 1738
BsrFI RCCGGY 1 cut(s) 313
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 5 cut(s) 448, 1011, 1060, 1448, 1488
BssMI GATC 5 cut(s) 745, 827, 1080, 1102, 1616
BssNAI GTATAC 1 cut(s) 1404
BssNI GRCGYC 2 cut(s) 183, 240
BssT1I CCWWGG 1 cut(s) 1448
Bst1107I GTATAC 1 cut(s) 1404
Bst2UI CCWGG 4 cut(s) 463, 941, 1043, 1490
Bst4CI ACNGT 5 cut(s) 715, 823, 1061, 1415, 1475
Bst6I CTCTTC 3 cut(s) 230, 699, 976
BstACI GRCGYC 2 cut(s) 183, 240
BstC8I GCNNGC 5 cut(s) 611, 843, 1150, 1438, 1653
BstDEI CTNAG 7 cut(s) 10, 58, 137, 483, 852, 1073, 1276
BstDSI CCRYGG 2 cut(s) 448, 1060
BstF5I GGATG 2 cut(s) 583, 741
BstFNI CGCG 2 cut(s) 412, 450
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 5 cut(s) 748, 830, 1083, 1105, 1619
BstMAI GTCTC 3 cut(s) 13, 207, 304
BstMBI GATC 5 cut(s) 745, 827, 1080, 1102, 1616
BstMWI GCNNNNNNNGC 4 cut(s) 11, 36, 128, 323
BstNI CCWGG 4 cut(s) 463, 941, 1043, 1490
BstNSI RCATGY 2 cut(s) 1312, 1486
BstSCI CCNGG 8 cut(s) 353, 461, 939, 1010, 1011, 1041, 1135, 1488
BstSFI CTRYAG 2 cut(s) 1338, 1656
BstUI CGCG 2 cut(s) 412, 450
BstV1I GCAGC 4 cut(s) 277, 1324, 1642, 1670
BstV2I GAAGAC 1 cut(s) 1266
BstX2I RGATCY 3 cut(s) 745, 1080, 1102
BstYI RGATCY 3 cut(s) 745, 1080, 1102
BstZ17I GTATAC 1 cut(s) 1404
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 2 cut(s) 448, 1060
BtgZI GCGATG 1 cut(s) 1236
BtrI CACGTC 1 cut(s) 1133
BtsCI GGATG 2 cut(s) 583, 741
BtsI GCAGTG 3 cut(s) 535, 729, 1447
BtsIMutI CAGTG 4 cut(s) 535, 729, 1447, 1480
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 5 cut(s) 611, 843, 1150, 1438, 1653
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 7 cut(s) 171, 346, 452, 603, 1039, 1445, 1745
Cfr42I CCGCGG 1 cut(s) 451
Cfr9I CCCGGG 1 cut(s) 1011
CpoI CGGWCCG 1 cut(s) 452
CseI GACGC 4 cut(s) 142, 191, 248, 296
CsiI ACCWGGT 1 cut(s) 939
Csp6I GTAC 4 cut(s) 597, 804, 1558, 1642
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CspI CGGWCCG 1 cut(s) 452
CviAII CATG 5 cut(s) 600, 1195, 1309, 1418, 1483
CviQI GTAC 4 cut(s) 597, 804, 1558, 1642
DdeI CTNAG 7 cut(s) 10, 58, 137, 483, 852, 1073, 1276
DpnI GATC 5 cut(s) 747, 829, 1082, 1104, 1618
DpnII GATC 5 cut(s) 745, 827, 1080, 1102, 1616
Eam1104I CTCTTC 3 cut(s) 230, 699, 976
EarI CTCTTC 3 cut(s) 230, 699, 976
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 1448
Eco24I GRGCYC 1 cut(s) 1074
Eco31I GGTCTC 1 cut(s) 304
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 6 cut(s) 171, 452, 603, 1039, 1445, 1745
Eco47III AGCGCT 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 3 cut(s) 852, 1106, 1407
Eco88I CYCGRG 3 cut(s) 421, 799, 1011
EcoICRI GAGCTC 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 1745
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 4 cut(s) 461, 939, 1041, 1488
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 1 cut(s) 1448
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 1448
FaeI CATG 5 cut(s) 603, 1198, 1312, 1421, 1486
FalI AAGNNNNNCTT 2 cut(s) 1297, 1329
FaqI GGGAC 2 cut(s) 394, 994
FatI CATG 5 cut(s) 599, 1194, 1308, 1417, 1482
FauI CCCGC 4 cut(s) 141, 388, 405, 455
FblI GTMKAC 2 cut(s) 417, 1403
Fnu4HI GCNGC 6 cut(s) 45, 291, 349, 1338, 1656, 1659
FokI GGATG 2 cut(s) 590, 748
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 6 cut(s) 45, 291, 349, 1338, 1656, 1659
FspBI CTAG 5 cut(s) 266, 807, 1023, 1149, 1449
GlaI GCGC 1 cut(s) 160
GluI GCNGC 6 cut(s) 45, 291, 349, 1338, 1656, 1659
GsaI CCCAGC 1 cut(s) 122
GsuI CTGGAG 2 cut(s) 717, 882
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 348
HapII CCGG 5 cut(s) 314, 354, 455, 1012, 1136
HgaI GACGC 4 cut(s) 142, 191, 248, 296
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 2 cut(s) 183, 240
Hin1II CATG 5 cut(s) 603, 1198, 1312, 1421, 1486
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 3 cut(s) 234, 253, 418
HindII GTYRAC 3 cut(s) 234, 253, 418
HindIII AAGCTT 3 cut(s) 196, 281, 998
HinfI GANTC 7 cut(s) 254, 305, 335, 458, 623, 856, 1706
HpaII CCGG 5 cut(s) 314, 354, 455, 1012, 1136
HphI GGTGA 2 cut(s) 1336, 1649
Hpy166II GTNNAC 6 cut(s) 234, 253, 418, 731, 1064, 1404
Hpy8I GTNNAC 6 cut(s) 234, 253, 418, 731, 1064, 1404
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 6 cut(s) 25, 336, 382, 879, 1081, 1155
HpyCH4III ACNGT 5 cut(s) 715, 823, 1061, 1415, 1475
HpyCH4IV ACGT 2 cut(s) 1132, 1177
HpyCH4V TGCA 9 cut(s) 293, 632, 649, 908, 956, 1340, 1606, 1651, 1658
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 36, 128, 323
HpyF3I CTNAG 7 cut(s) 10, 58, 137, 483, 852, 1073, 1276
HpySE526I ACGT 2 cut(s) 1132, 1177
Hsp92I GRCGYC 2 cut(s) 183, 240
Hsp92II CATG 5 cut(s) 603, 1198, 1312, 1421, 1486
HspAI GCGC 1 cut(s) 159
Kpn2I TCCGGA 1 cut(s) 454
KspI CCGCGG 1 cut(s) 451
Kzo9I GATC 5 cut(s) 745, 827, 1080, 1102, 1616
LmnI GCTCC 8 cut(s) 36, 356, 402, 443, 698, 901, 1054, 1453
Lsp1109I GCAGC 4 cut(s) 277, 1324, 1642, 1670
LweI GCATC 3 cut(s) 71, 361, 619
MabI ACCWGGT 1 cut(s) 939
MaeI CTAG 5 cut(s) 266, 807, 1023, 1149, 1449
MaeII ACGT 2 cut(s) 1132, 1177
MaeIII GTNAC 3 cut(s) 488, 1342, 1752
MalI GATC 5 cut(s) 747, 829, 1082, 1104, 1618
MbiI CCGCTC 4 cut(s) 136, 351, 438, 1270
MboI GATC 5 cut(s) 745, 827, 1080, 1102, 1616
MboII GAAGA 9 cut(s) 217, 677, 716, 800, 993, 1152, 1177, 1199, 1271
MflI RGATCY 3 cut(s) 745, 1080, 1102
MhlI GDGCHC 2 cut(s) 1059, 1074
MluCI AATT 9 cut(s) 52, 364, 478, 634, 658, 962, 1581, 1601, 1645
MlyI GAGTC 2 cut(s) 248, 1715
MmeI TCCRAC 3 cut(s) 380, 410, 555
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 3 cut(s) 562, 822, 1095
MseI TTAA 2 cut(s) 717, 1385
MspA1I CMGCKG 3 cut(s) 191, 326, 450
MspI CCGG 5 cut(s) 314, 354, 455, 1012, 1136
MspR9I CCNGG 8 cut(s) 355, 463, 941, 1012, 1013, 1043, 1137, 1490
Mva1269I GAATGC 2 cut(s) 613, 754
MvaI CCWGG 4 cut(s) 463, 941, 1043, 1490
MvnI CGCG 2 cut(s) 412, 450
MwoI GCNNNNNNNGC 4 cut(s) 11, 36, 128, 323
NciI CCSGG 4 cut(s) 355, 1012, 1013, 1137
NdeII GATC 5 cut(s) 745, 827, 1080, 1102, 1616
NheI GCTAGC 1 cut(s) 1148
NlaIII CATG 5 cut(s) 603, 1198, 1312, 1421, 1486
NlaIV GGNNCC 4 cut(s) 347, 434, 1082, 1455
NmuCI GTSAC 3 cut(s) 488, 1342, 1752
NspI RCATGY 2 cut(s) 1312, 1486
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 305, 314
PctI GAATGC 2 cut(s) 613, 754
PdmI GAANNNNTTC 3 cut(s) 562, 822, 1095
PfeI GAWTC 5 cut(s) 305, 335, 458, 623, 856
PfoI TCCNGGA 2 cut(s) 461, 1135
PkrI GCNGC 6 cut(s) 46, 292, 350, 1339, 1657, 1660
PleI GAGTC 2 cut(s) 248, 1714
PpsI GAGTC 2 cut(s) 248, 1714
PpuMI RGGWCCY 1 cut(s) 1745
PshBI ATTAAT 1 cut(s) 1385
Psp124BI GAGCTC 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 1745
Psp6I CCWGG 4 cut(s) 461, 939, 1041, 1488
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 4 cut(s) 461, 939, 1041, 1488
PspN4I GGNNCC 4 cut(s) 347, 434, 1082, 1455
PspPI GGNCC 7 cut(s) 171, 346, 452, 603, 1039, 1445, 1745
PspPPI RGGWCCY 1 cut(s) 1745
PstI CTGCAG 2 cut(s) 1342, 1660
PsuI RGATCY 3 cut(s) 745, 1080, 1102
RsaI GTAC 4 cut(s) 598, 805, 1559, 1643
RsaNI GTAC 4 cut(s) 597, 804, 1558, 1642
Rsr2I CGGWCCG 1 cut(s) 452
RsrII CGGWCCG 1 cut(s) 452
SacI GAGCTC 1 cut(s) 1074
SacII CCGCGG 1 cut(s) 451
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 2 cut(s) 717, 1385
SatI GCNGC 6 cut(s) 45, 291, 349, 1338, 1656, 1659
Sau3AI GATC 5 cut(s) 745, 827, 1080, 1102, 1616
Sau96I GGNCC 7 cut(s) 171, 346, 452, 603, 1039, 1445, 1745
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 2 cut(s) 248, 1715
ScrFI CCNGG 8 cut(s) 355, 463, 941, 1012, 1013, 1043, 1137, 1490
SduI GDGCHC 2 cut(s) 1059, 1074
SexAI ACCWGGT 1 cut(s) 939
SfaNI GCATC 3 cut(s) 71, 361, 619
SfcI CTRYAG 2 cut(s) 1338, 1656
Sfr303I CCGCGG 1 cut(s) 451
SgrBI CCGCGG 1 cut(s) 451
SinI GGWCC 6 cut(s) 171, 452, 603, 1039, 1445, 1745
SmaI CCCGGG 1 cut(s) 1013
SmlI CTYRAG 3 cut(s) 166, 1001, 1532
SmoI CTYRAG 3 cut(s) 166, 1001, 1532
SpeI ACTAGT 1 cut(s) 806
Sse9I AATT 9 cut(s) 52, 364, 478, 634, 658, 962, 1581, 1601, 1645
SspMI CTAG 5 cut(s) 266, 807, 1023, 1149, 1449
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 8 cut(s) 353, 461, 939, 1010, 1011, 1041, 1135, 1488
StyI CCWWGG 1 cut(s) 1448
TaaI ACNGT 5 cut(s) 715, 823, 1061, 1415, 1475
TaiI ACGT 2 cut(s) 1135, 1180
TaqI TCGA 5 cut(s) 299, 308, 417, 826, 1677
TasI AATT 9 cut(s) 52, 364, 478, 634, 658, 962, 1581, 1601, 1645
TatI WGTACW 2 cut(s) 803, 1641
TauI GCSGC 2 cut(s) 47, 351
TfiI GAWTC 5 cut(s) 305, 335, 458, 623, 856
Tru1I TTAA 2 cut(s) 717, 1385
Tru9I TTAA 2 cut(s) 717, 1385
TscAI CASTG 4 cut(s) 535, 729, 1447, 1480
TseFI GTSAC 3 cut(s) 488, 1342, 1752
TseI GCWGC 4 cut(s) 290, 1337, 1655, 1658
Tsp45I GTSAC 3 cut(s) 488, 1342, 1752
TspDTI ATGAA 5 cut(s) 510, 678, 752, 831, 1568
TspGWI ACGGA 3 cut(s) 251, 748, 1613
TspMI CCCGGG 1 cut(s) 1011
TspRI CASTG 4 cut(s) 535, 729, 1447, 1480
VpaK11BI GGWCC 6 cut(s) 171, 452, 603, 1039, 1445, 1745
VspI ATTAAT 1 cut(s) 1385
XapI RAATTY 2 cut(s) 364, 634
XbaI TCTAGA 1 cut(s) 1022
XceI RCATGY 2 cut(s) 1312, 1486
XmaI CCCGGG 1 cut(s) 1011
XmaJI CCTAGG 1 cut(s) 1448
XmiI GTMKAC 2 cut(s) 417, 1403
XmnI GAANNNNTTC 3 cut(s) 562, 822, 1095
XspI CTAG 5 cut(s) 266, 807, 1023, 1149, 1449
ZrmI AGTACT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.