pycom02g22660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016381
Physical Location & Seq
Forward (+)
20872448 .. 20880080
7633 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22660.3

Sequence Viewer

Length: 1809 bp
ATGGCAGTCTCAGCCTTCAAATCTTCAACCAAAAGAAGCACCGCCACCACCACCACCACCACCTCCAACTTCTCAGCACCGTCGTCATCTTCGTCATCTGCCGGCAGACAAATCACCGACAAAGATAATCCACCGCCAAAGAAAGCCCCAATCAGACGGTCAAGAAGCGTCAGCGCTTTCTCAAGGACCAGCCTTGACGCCTCTGCTGAAAGTTTCTTGAACAAAAGAGACAACCCTCTTTTCTGGTCTACTGACTCCCAAGCCGTTGACTCTCAGAACCTAGTCAAACCCGCCAAATTCGACTCTGTAACGTCCAAAGATTCGAGACCTGCCACCTTCACTGGCGCTGATACTAGAAGGGGCCGATCCGCTGCGCGGAATGCCGACGCCTTGGGGACCCGGAAGGAGGTGGGTCGGAGCTTGTCGAGGGTCGACCCGGGACGCCGGAACCGCTCCGTTTCGCGTGGTCCGGATTCCAGGCGGAGTTTCGTCAATCCCAAGAGTGAGGATGAACAAGAATGCGGTTCATTGAGTAAATTGGGCAATGATAGGAAAAAGGGTGGTTTAGTTAGAAGTAGTTCTGCTCTGTTGGATCAGGTGAAAGGTCTGCGGACGTGGTCCAGTCAGCATTCGCAGGAATTGGATGCAAATTTGTCTTCGTCACAGTCTCTAAATTGGGAAGATGGAGTTTCGGTTGCAAGTTCTTTGTCTGGAGCTGAAGAGAAGACTGTTAAAGCAGTTTGTGAACAGTTGAATGTGAACTGCGGGCGTCCTTTCAATGATTTGTCAGTTCAAGGGAATCATTTGGAAGGGGATACCACTACCAGTAGTATATATGAAACAGTTCGTTCTGAAGTGCGAAGAGCTATATCTGAGATTCAAAACGACCTTGCAAGTGCTATGCAAAGGAGTAATACTCCAGCACTTGCAAATACTGACATTTCTGATATCCCTCCTGACCTGGTAAATCCAAGTGCAGTTGAATTAGTTTTGGACATCAGAAGAGAGTATGCCAAAAAGCTGGAGCAGTCACAGGAACGCGCTAGAAAACTTCAATCAGATCTGGCTGTTGAGGAGCACCGTGCACAAGAGCTCAGCAGAATTCTGAAGGAAGTTCTTCCAGATCCCAAGACCCCTAGTGTGCCAAAGTCTCGCCCAGGAAGAAAAGCTAGCATTGAAAGAAGAAAGATGTCCAAACGTCTAACGGATGAAGCCATGGCTTATTTTGATGAGTGTGTATCCCTATCAACTTTTGACAGTTCCGACTTCTCATCCCCAGAGGACCCACCATTCAACTTAACTGGCTTTACTACCCCAGTCAGAAAAGGCTCATCTTTACTCCAAGCAAATTCAGTTGCTTCAGTCACCAATAGCTCGAACTTTTGCCTCAATGATTTAAAGGAGTTGAACATCGAACGTCAGTTTTCCAATGAACGGCATGCTTCAGGACTGATGGCCAGCGGTGGTCCTAGGGAGCCAGCTCTGAATCAAATTAGTCCTAACAGTGCCAAGAGAGGCCAGGATTGGAAGTTCTCGTTTGCTCATAAGCCAACAGCAGAAACCCTTGATCTTCAACAGGACATTAAGAAGTATGTCCGACATTTTGAAAAAGGAAATGAGAAAGATGACACGGAATTGCAGATTGTACGATCAAACTATTGTGATTTGGGTGACTATAAATTGCAGGCTGCAGCACAAAGCTATATGCTCGACAGGGTCTTGTTTAGAAACAGAATACAGACGGGTGGTATTTTACTCTGCGGTACTGGTATTTCGAACCCCTTTTCCCCATTTGCTTCATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

603

Amino Acids

66.13

Weight (kDa)

9.25

Isoelectric Point (pI)

63.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016381)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04960 FvH4_7g05350
malus_domestica MD02G1265200.v1.1
prunus_persica Prupe.2G060300_v2.0.a1
pyrus_communis pycom02g22660
rosa_chinensis RchiOBHm_Chr1g0331161
rosa_laevigata RLG00000029749
rosa_multiflora Rmu_sc0006123.1_g000009
rosa_roxburghii Rroxscaffold_4G00319310
rosa_rugosa Rorug01G0091800
rosa_samantha Rh1AG114800 Rh1BG087500 Rh1CG110800 Rh1DG119500
rosa_wichuraiana Rw1G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AccBSI CCGCTC 1 cut(s) 453
AccI GTMKAC 2 cut(s) 248, 432
AccII CGCG 3 cut(s) 376, 463, 1041
AccIII TCCGGA 1 cut(s) 469
AclWI GGATC 3 cut(s) 360, 600, 1118
AcoI YGGCCR 1 cut(s) 1455
AcsI RAATTY 4 cut(s) 296, 649, 1101, 1348
AcuI CTGAAG 5 cut(s) 738, 873, 1127, 1344, 1428
AcyI GRCGYC 4 cut(s) 198, 387, 442, 769
AfaI GTAC 2 cut(s) 1647, 1765
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 3 cut(s) 375, 406, 1434
AjiI CACGTC 1 cut(s) 615
AjnI CCWGG 4 cut(s) 476, 960, 1156, 1518
AloI GAACNNNNNNTCC 4 cut(s) 1514, 1546, 1769, 1801
AluBI AGCT 9 cut(s) 420, 716, 866, 1021, 1093, 1169, 1374, 1481, 1701
AluI AGCT 9 cut(s) 420, 716, 866, 1021, 1093, 1169, 1374, 1481, 1701
Alw21I GWGCWC 3 cut(s) 1080, 1087, 1095
Alw26I GTCTC 5 cut(s) 13, 222, 319, 672, 1155
Alw44I GTGCAC 1 cut(s) 1083
AlwI GGATC 3 cut(s) 360, 600, 1118
Ama87I CYCGRG 1 cut(s) 436
Aor13HI TCCGGA 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 3 cut(s) 361, 1455, 1516
ApaLI GTGCAC 1 cut(s) 1083
ApeKI GCWGC 3 cut(s) 371, 1688, 1691
ApoI RAATTY 4 cut(s) 296, 649, 1101, 1348
Asp700I GAANNNNTTC 3 cut(s) 577, 843, 1116
AspA2I CCTAGG 1 cut(s) 1469
AspLEI GCGC 4 cut(s) 176, 347, 376, 1043
AspS9I GGNCC 7 cut(s) 186, 361, 396, 467, 618, 1282, 1466
AsuC2I CCSGG 3 cut(s) 400, 437, 438
AsuHPI GGTGA 4 cut(s) 106, 610, 1357, 1682
AsuII TTCGAA 1 cut(s) 1775
AsuNHI GCTAGC 1 cut(s) 1169
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 6 cut(s) 186, 396, 467, 618, 1282, 1466
AvrII CCTAGG 1 cut(s) 1469
BaeGI GKGCMC 1 cut(s) 1087
BalI TGGCCA 1 cut(s) 1457
BanII GRGCYC 1 cut(s) 1095
BbsI GAAGAC 2 cut(s) 648, 731
Bbv12I GWGCWC 3 cut(s) 1080, 1087, 1095
BbvI GCAGC 3 cut(s) 358, 1675, 1703
BccI CCATC 2 cut(s) 677, 1447
BceAI ACGGC 2 cut(s) 248, 1451
BciT130I CCWGG 4 cut(s) 478, 962, 1158, 1520
BciVI GTATCC 2 cut(s) 808, 1249
BcnI CCSGG 3 cut(s) 400, 437, 438
BcoDI GTCTC 5 cut(s) 13, 222, 319, 672, 1155
BfaI CTAG 6 cut(s) 281, 354, 1044, 1137, 1170, 1470
BfmI CTRYAG 1 cut(s) 1689
BfoI RGCGCY 2 cut(s) 177, 348
BfuAI ACCTGC 1 cut(s) 337
BfuI GTATCC 2 cut(s) 808, 1249
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 3 cut(s) 372, 1689, 1692
BlnI CCTAGG 1 cut(s) 1469
BlpI GCTNAGC 1 cut(s) 1094
BlsI GCNGC 3 cut(s) 373, 1690, 1693
Bme1390I CCNGG 7 cut(s) 400, 437, 438, 478, 962, 1158, 1520
Bme18I GGWCC 6 cut(s) 186, 396, 467, 618, 1282, 1466
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 615
BmgT120I GGNCC 7 cut(s) 186, 361, 396, 467, 618, 1282, 1466
BmiI GGNNCC 6 cut(s) 362, 397, 398, 449, 1284, 1476
BmrFI CCNGG 7 cut(s) 400, 437, 438, 478, 962, 1158, 1520
BmrI ACTGGG 1 cut(s) 1310
BmsI GCATC 1 cut(s) 634
BmtI GCTAGC 1 cut(s) 1173
BmuI ACTGGG 1 cut(s) 1310
BpiI GAAGAC 2 cut(s) 648, 731
BplI GAGNNNNNCTC 2 cut(s) 901, 933
BpmI CTGGAG 3 cut(s) 732, 903, 1043
Bpu1102I GCTNAGC 1 cut(s) 1094
Bpu14I TTCGAA 1 cut(s) 1775
BpuEI CTTGAG 1 cut(s) 166
BpuMI CCSGG 3 cut(s) 400, 437, 438
BsaHI GRCGYC 4 cut(s) 198, 387, 442, 769
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 5 cut(s) 390, 436, 1156, 1215, 1469
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bsc4I CCNNNNNNNGG 3 cut(s) 375, 406, 1434
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 6 cut(s) 346, 621, 825, 1306, 1316, 1771
Bse3DI GCAATG 1 cut(s) 550
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 4 cut(s) 478, 962, 1158, 1520
BseDI CCNNGG 5 cut(s) 390, 436, 1156, 1215, 1469
BseGI GGATG 4 cut(s) 514, 649, 1213, 1271
BseLI CCNNNNNNNGG 3 cut(s) 375, 406, 1434
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 5 cut(s) 24, 87, 287, 864, 1108
BseNI ACTGG 6 cut(s) 346, 621, 825, 1306, 1316, 1771
BseRI GAGGAG 1 cut(s) 1088
BseSI GKGCMC 1 cut(s) 1087
BseXI GCAGC 3 cut(s) 358, 1675, 1703
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 3 cut(s) 376, 463, 1041
BshFI GGCC 3 cut(s) 363, 1457, 1518
BsiHKAI GWGCWC 3 cut(s) 1080, 1087, 1095
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 5 cut(s) 102, 400, 437, 445, 470
BslFI GGGAC 2 cut(s) 409, 453
BslI CCNNNNNNNGG 3 cut(s) 375, 406, 1434
BsmAI GTCTC 5 cut(s) 13, 222, 319, 672, 1155
BsmFI GGGAC 2 cut(s) 409, 453
BsmI GAATGC 3 cut(s) 385, 524, 628
BsnI GGCC 3 cut(s) 363, 1457, 1518
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 1775
Bsp1286I GDGCHC 3 cut(s) 1080, 1087, 1095
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
Bsp1720I GCTNAGC 1 cut(s) 1094
Bsp19I CCATGG 1 cut(s) 1215
BspANI GGCC 3 cut(s) 363, 1457, 1518
BspCNI CTCAG 5 cut(s) 23, 86, 286, 865, 1107
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 3 cut(s) 376, 463, 1041
BspLI GGNNCC 6 cut(s) 362, 397, 398, 449, 1284, 1476
BspMAI CTGCAG 1 cut(s) 1693
BspMI ACCTGC 1 cut(s) 337
BspOI GCTAGC 1 cut(s) 1173
BspPI GGATC 3 cut(s) 360, 600, 1118
BspQI GCTCTTC 1 cut(s) 856
BspT104I TTCGAA 1 cut(s) 1775
BspTNI GGTCTC 1 cut(s) 319
BsrBI CCGCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 6 cut(s) 346, 621, 825, 1306, 1316, 1771
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 5 cut(s) 390, 436, 1156, 1215, 1469
BssMI GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
BssNI GRCGYC 4 cut(s) 198, 387, 442, 769
BssT1I CCWWGG 3 cut(s) 390, 1215, 1469
Bst2UI CCWGG 4 cut(s) 478, 962, 1158, 1520
Bst4CI ACNGT 9 cut(s) 81, 159, 666, 730, 750, 844, 1082, 1259, 1505
Bst6I CTCTTC 3 cut(s) 714, 856, 997
BstACI GRCGYC 4 cut(s) 198, 387, 442, 769
BstBI TTCGAA 1 cut(s) 1775
BstC8I GCNNGC 7 cut(s) 103, 767, 1171, 1440, 1459, 1479, 1686
BstDEI CTNAG 5 cut(s) 10, 73, 273, 873, 1094
BstDSI CCRYGG 1 cut(s) 1215
BstF5I GGATG 4 cut(s) 514, 649, 1213, 1271
BstFNI CGCG 3 cut(s) 376, 463, 1041
BstH2I RGCGCY 2 cut(s) 177, 348
BstHHI GCGC 4 cut(s) 176, 347, 376, 1043
BstKTI GATC 6 cut(s) 368, 595, 1063, 1126, 1570, 1652
BstMAI GTCTC 5 cut(s) 13, 222, 319, 672, 1155
BstMBI GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
BstMWI GCNNNNNNNGC 3 cut(s) 11, 380, 450
BstNI CCWGG 4 cut(s) 478, 962, 1158, 1520
BstNSI RCATGY 1 cut(s) 1442
BstSCI CCNGG 7 cut(s) 398, 435, 436, 476, 960, 1156, 1518
BstSFI CTRYAG 1 cut(s) 1689
BstSLI GKGCMC 1 cut(s) 1087
BstUI CGCG 3 cut(s) 376, 463, 1041
BstV1I GCAGC 3 cut(s) 358, 1675, 1703
BstV2I GAAGAC 2 cut(s) 648, 731
BstX2I RGATCY 2 cut(s) 1060, 1123
BstXI CCANNNNNNTGG 1 cut(s) 1021
BstYI RGATCY 2 cut(s) 1060, 1123
BsuI GTATCC 2 cut(s) 808, 1249
BsuRI GGCC 3 cut(s) 363, 1457, 1518
BtgI CCRYGG 1 cut(s) 1215
BtrI CACGTC 1 cut(s) 615
BtsCI GGATG 4 cut(s) 514, 649, 1213, 1271
BtsIMutI CAGTG 2 cut(s) 339, 1510
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 7 cut(s) 103, 767, 1171, 1440, 1459, 1479, 1686
CfoI GCGC 4 cut(s) 176, 347, 376, 1043
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 7 cut(s) 186, 361, 396, 467, 618, 1282, 1466
Cfr9I CCCGGG 1 cut(s) 436
CseI GACGC 5 cut(s) 157, 206, 395, 450, 758
CsiI ACCWGGT 1 cut(s) 960
Csp6I GTAC 2 cut(s) 1646, 1764
CviAII CATG 2 cut(s) 1216, 1439
CviQI GTAC 2 cut(s) 1646, 1764
DdeI CTNAG 5 cut(s) 10, 73, 273, 873, 1094
DpnI GATC 6 cut(s) 367, 594, 1062, 1125, 1569, 1651
DpnII GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
DraI TTTAAA 1 cut(s) 1398
EaeI YGGCCR 1 cut(s) 1455
Eam1104I CTCTTC 3 cut(s) 714, 856, 997
EarI CTCTTC 3 cut(s) 714, 856, 997
EciI GGCGGA 1 cut(s) 496
Ecl136II GAGCTC 1 cut(s) 1093
Eco130I CCWWGG 3 cut(s) 390, 1215, 1469
Eco24I GRGCYC 1 cut(s) 1095
Eco31I GGTCTC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 6 cut(s) 186, 396, 467, 618, 1282, 1466
Eco47III AGCGCT 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 1093
Eco57I CTGAAG 5 cut(s) 738, 873, 1127, 1344, 1428
Eco88I CYCGRG 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 1093
EcoO109I RGGNCCY 2 cut(s) 396, 1282
EcoRI GAATTC 1 cut(s) 1101
EcoRII CCWGG 4 cut(s) 476, 960, 1156, 1518
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 3 cut(s) 390, 1215, 1469
EcoT38I GRGCYC 1 cut(s) 1095
ErhI CCWWGG 3 cut(s) 390, 1215, 1469
FaeI CATG 2 cut(s) 1219, 1442
FaqI GGGAC 2 cut(s) 409, 453
FatI CATG 2 cut(s) 1215, 1438
FauI CCCGC 2 cut(s) 298, 758
FblI GTMKAC 2 cut(s) 248, 432
Fnu4HI GCNGC 3 cut(s) 372, 1689, 1692
FokI GGATG 4 cut(s) 521, 656, 1220, 1258
FriOI GRGCYC 1 cut(s) 1095
Fsp4HI GCNGC 3 cut(s) 372, 1689, 1692
FspBI CTAG 6 cut(s) 281, 354, 1044, 1137, 1170, 1470
GlaI GCGC 4 cut(s) 175, 346, 375, 1042
GluI GCNGC 3 cut(s) 372, 1689, 1692
GsuI CTGGAG 3 cut(s) 732, 903, 1043
HaeII RGCGCY 2 cut(s) 177, 348
HaeIII GGCC 3 cut(s) 363, 1457, 1518
HapII CCGG 5 cut(s) 102, 400, 437, 445, 470
HgaI GACGC 5 cut(s) 157, 206, 395, 450, 758
HhaI GCGC 4 cut(s) 176, 347, 376, 1043
Hin1I GRCGYC 4 cut(s) 198, 387, 442, 769
Hin1II CATG 2 cut(s) 1219, 1442
Hin6I GCGC 4 cut(s) 174, 345, 374, 1041
HinP1I GCGC 4 cut(s) 174, 345, 374, 1041
HincII GTYRAC 2 cut(s) 268, 433
HindII GTYRAC 2 cut(s) 268, 433
HinfI GANTC 8 cut(s) 254, 269, 302, 320, 473, 799, 877, 1486
HpaII CCGG 5 cut(s) 102, 400, 437, 445, 470
HphI GGTGA 4 cut(s) 106, 610, 1357, 1682
Hpy166II GTNNAC 6 cut(s) 249, 268, 433, 746, 760, 1085
Hpy188III TCNNGA 8 cut(s) 162, 217, 324, 470, 711, 956, 1121, 1446
Hpy8I GTNNAC 6 cut(s) 249, 268, 433, 746, 760, 1085
Hpy99I CGWCG 2 cut(s) 85, 389
HpyAV CCTTC 6 cut(s) 25, 346, 351, 397, 803, 1102
HpyCH4III ACNGT 9 cut(s) 81, 159, 666, 730, 750, 844, 1082, 1259, 1505
HpyCH4IV ACGT 4 cut(s) 311, 614, 1198, 1417
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 380, 450
HpyF3I CTNAG 5 cut(s) 10, 73, 273, 873, 1094
HpySE526I ACGT 4 cut(s) 311, 614, 1198, 1417
Hsp92I GRCGYC 4 cut(s) 198, 387, 442, 769
Hsp92II CATG 2 cut(s) 1219, 1442
HspAI GCGC 4 cut(s) 174, 345, 374, 1041
KflI GGGWCCC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 469
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
LguI GCTCTTC 1 cut(s) 856
LmnI GCTCC 6 cut(s) 417, 458, 713, 1024, 1075, 1474
Lsp1109I GCAGC 3 cut(s) 358, 1675, 1703
LweI GCATC 1 cut(s) 634
MabI ACCWGGT 1 cut(s) 960
MaeI CTAG 6 cut(s) 281, 354, 1044, 1137, 1170, 1470
MaeII ACGT 4 cut(s) 311, 614, 1198, 1417
MaeIII GTNAC 5 cut(s) 307, 660, 1029, 1363, 1670
MalI GATC 6 cut(s) 367, 594, 1062, 1125, 1569, 1651
MbiI CCGCTC 1 cut(s) 453
MboI GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
MflI RGATCY 2 cut(s) 1060, 1123
MhlI GDGCHC 3 cut(s) 1080, 1087, 1095
MlsI TGGCCA 1 cut(s) 1457
MluNI TGGCCA 1 cut(s) 1457
MlyI GAGTC 3 cut(s) 248, 263, 296
MmeI TCCRAC 5 cut(s) 90, 395, 570, 1287, 1621
Mox20I TGGCCA 1 cut(s) 1457
MroI TCCGGA 1 cut(s) 469
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 3 cut(s) 577, 843, 1116
MscI TGGCCA 1 cut(s) 1457
MseI TTAA 4 cut(s) 732, 1298, 1397, 1584
Msp20I TGGCCA 1 cut(s) 1457
MspA1I CMGCKG 2 cut(s) 371, 1461
MspI CCGG 5 cut(s) 102, 400, 437, 445, 470
MspR9I CCNGG 7 cut(s) 400, 437, 438, 478, 962, 1158, 1520
Mva1269I GAATGC 3 cut(s) 385, 524, 628
MvaI CCWGG 4 cut(s) 478, 962, 1158, 1520
MvnI CGCG 3 cut(s) 376, 463, 1041
MwoI GCNNNNNNNGC 3 cut(s) 11, 380, 450
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 3 cut(s) 400, 437, 438
NcoI CCATGG 1 cut(s) 1215
NdeII GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
NgoMIV GCCGGC 1 cut(s) 101
NheI GCTAGC 1 cut(s) 1169
NlaIII CATG 2 cut(s) 1219, 1442
NlaIV GGNNCC 6 cut(s) 362, 397, 398, 449, 1284, 1476
NmuCI GTSAC 4 cut(s) 660, 1029, 1363, 1670
NspI RCATGY 1 cut(s) 1442
NspV TTCGAA 1 cut(s) 1775
PaeI GCATGC 1 cut(s) 1442
PciSI GCTCTTC 1 cut(s) 856
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 3 cut(s) 385, 524, 628
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 3 cut(s) 577, 843, 1116
PfeI GAWTC 5 cut(s) 320, 473, 799, 877, 1486
PflFI GACNNNGTC 2 cut(s) 616, 1715
PkrI GCNGC 3 cut(s) 373, 1690, 1693
PleI GAGTC 3 cut(s) 248, 263, 296
PpsI GAGTC 3 cut(s) 248, 263, 296
PpuMI RGGWCCY 2 cut(s) 396, 1282
Psp124BI GAGCTC 1 cut(s) 1095
Psp5II RGGWCCY 2 cut(s) 396, 1282
Psp6I CCWGG 4 cut(s) 476, 960, 1156, 1518
PspGI CCWGG 4 cut(s) 476, 960, 1156, 1518
PspN4I GGNNCC 6 cut(s) 362, 397, 398, 449, 1284, 1476
PspPI GGNCC 7 cut(s) 186, 361, 396, 467, 618, 1282, 1466
PspPPI RGGWCCY 2 cut(s) 396, 1282
PstI CTGCAG 1 cut(s) 1693
PsuI RGATCY 2 cut(s) 1060, 1123
PsyI GACNNNGTC 2 cut(s) 616, 1715
RsaI GTAC 2 cut(s) 1647, 1765
RsaNI GTAC 2 cut(s) 1646, 1764
SacI GAGCTC 1 cut(s) 1095
SalI GTCGAC 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 856
SaqAI TTAA 4 cut(s) 732, 1298, 1397, 1584
SatI GCNGC 3 cut(s) 372, 1689, 1692
Sau3AI GATC 6 cut(s) 365, 592, 1060, 1123, 1567, 1649
Sau96I GGNCC 7 cut(s) 186, 361, 396, 467, 618, 1282, 1466
SchI GAGTC 3 cut(s) 248, 263, 296
ScrFI CCNGG 7 cut(s) 400, 437, 438, 478, 962, 1158, 1520
SduI GDGCHC 3 cut(s) 1080, 1087, 1095
SexAI ACCWGGT 1 cut(s) 960
SfaNI GCATC 1 cut(s) 634
SfcI CTRYAG 1 cut(s) 1689
SfuI TTCGAA 1 cut(s) 1775
SinI GGWCC 6 cut(s) 186, 396, 467, 618, 1282, 1466
SmaI CCCGGG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SphI GCATGC 1 cut(s) 1442
SspMI CTAG 6 cut(s) 281, 354, 1044, 1137, 1170, 1470
SstI GAGCTC 1 cut(s) 1095
StyD4I CCNGG 7 cut(s) 398, 435, 436, 476, 960, 1156, 1518
StyI CCWWGG 3 cut(s) 390, 1215, 1469
TaaI ACNGT 9 cut(s) 81, 159, 666, 730, 750, 844, 1082, 1259, 1505
TaiI ACGT 4 cut(s) 314, 617, 1201, 1420
TaqI TCGA 8 cut(s) 300, 323, 425, 432, 1376, 1413, 1710, 1775
TfiI GAWTC 5 cut(s) 320, 473, 799, 877, 1486
Tru1I TTAA 4 cut(s) 732, 1298, 1397, 1584
Tru9I TTAA 4 cut(s) 732, 1298, 1397, 1584
TscAI CASTG 2 cut(s) 346, 1510
TseFI GTSAC 4 cut(s) 660, 1029, 1363, 1670
TseI GCWGC 3 cut(s) 371, 1688, 1691
Tsp45I GTSAC 4 cut(s) 660, 1029, 1363, 1670
TspDTI ATGAA 6 cut(s) 516, 525, 852, 1224, 1446, 1788
TspGWI ACGGA 3 cut(s) 445, 1220, 1646
TspMI CCCGGG 1 cut(s) 436
TspRI CASTG 2 cut(s) 346, 1510
Tth111I GACNNNGTC 2 cut(s) 616, 1715
VneI GTGCAC 1 cut(s) 1083
VpaK11BI GGWCC 6 cut(s) 186, 396, 467, 618, 1282, 1466
XapI RAATTY 4 cut(s) 296, 649, 1101, 1348
XceI RCATGY 1 cut(s) 1442
XmaI CCCGGG 1 cut(s) 436
XmaJI CCTAGG 1 cut(s) 1469
XmiI GTMKAC 2 cut(s) 248, 432
XmnI GAANNNNTTC 3 cut(s) 577, 843, 1116
XspI CTAG 6 cut(s) 281, 354, 1044, 1137, 1170, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.