Rw1G009300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016381
Physical Location & Seq
Reverse (-)
20473109 .. 20479045
5937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009300.1

Sequence Viewer

Length: 1230 bp
ATGGCGGTCTCGGCTTTCAAATCCTCCACCAGAAGAAGCAATCCCGCCGGTGCCTCAACTTCATCATCATCATCATCATCAGCTATTTCTAAACGGAGCACCGATATAGATACTAGTTCAACCAAGAAACCAAACCCACCAATCAGAAGATCCAGAAGTGTCAGCGCTTTCTCCAGAAGTGTCAGCACTCTCGACTCTCAAGACAATATTTTCTTGAACAAGAGAGACAACCCTCTCTTCTGGTCTACGGATTCCCCAACCGCTGTTGACCCCAAAACCCCGCTAGTCAAACCCGACAAAGATTCGAGGCCGGCAACCTCGTCCAGTGCGGCCGATACTAGAAGGGGACGGTCGGTGTCGCGGAACGCCGATGTTTTGGTCGGTCGGAGCCAGTCCAGAGTTGACTCGGGTCGGAGCCTGTCCAGAGTCGACACTGGCCGTCGCAACTGCTCCGTTTCCCGGTGTCCGGAGCCCAGGGGGCGTTCCGTCATTTCCAAGAGTGAAGATGAACAAGAATGTACTTCTACATTGAATAAAGGGGAGGAAGGCAAAAAGGGTGGGTTAGTTAGAACTAGCTCTGATGAGTTGGATCAGTTGAAAGGCTTAAGGACATGGTCCAGTCAGCATTCGCCTTTCCAGCCTTCACATGCAAACTTGTCCAGCTTGCAATCTTCGAATTGGGAAGACGGAGTTTCCACCGCAAGTTCCTTATCTGGAGCCGAAGAGAAAACTTTTAAAGCAGCTTGTGAACAGATGAATATGAATTATGGACATTCGGGTGATGATTTGTCGGTTCAAGCAGTTCATTTGGAAAATGATACCACAAGCAGCAATATATATGAAACAGTTCGATCTGAAGTGAGACGTGCTATCTCTGAGATTCAAATTGACCTTGAAAGTGCTATGCGAAGGGGCAATACTCCGGCGCTGGCAACTTCTGACGTTTCTGATATCCCTCCTGACCTGGTAAATCCAAGTGCAGTTGAATTAGTTTTGGACATCAGAAGAGAATATGCCAAAAAACTGGAGCAGTCCAAGGAACGGGCTAGCAAACTTCAATCAGATCTGGCTGTTGAGGAGCACCGTGGACAAGAGCTCAGCAGAATCCTGAAGGAAGTACTTCCAGATCCCAAGATATCTAATGCGCCAAAATCTCGTCCTGGAAGAAAAGTAGGAATTTTTTTCTTCTCCTTAAAATCCCTCCTAGTAGTTCGGTTAATTTCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

44.6

Weight (kDa)

9.45

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016381)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04960 FvH4_7g05350
malus_domestica MD02G1265200.v1.1
prunus_persica Prupe.2G060300_v2.0.a1
pyrus_communis pycom02g22660
rosa_chinensis RchiOBHm_Chr1g0331161
rosa_laevigata RLG00000029749
rosa_multiflora Rmu_sc0006123.1_g000009
rosa_roxburghii Rroxscaffold_4G00319310
rosa_rugosa Rorug01G0091800
rosa_samantha Rh1AG114800 Rh1BG087500 Rh1CG110800 Rh1DG119500
rosa_wichuraiana Rw1G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AccI GTMKAC 2 cut(s) 245, 429
AccII CGCG 1 cut(s) 361
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 7 cut(s) 5, 45, 261, 281, 329, 361, 699
AclWI GGATC 3 cut(s) 144, 597, 1121
AcoI YGGCCR 2 cut(s) 330, 436
AcsI RAATTY 1 cut(s) 1176
AcuI CTGAAG 2 cut(s) 876, 1130
AfaI GTAC 2 cut(s) 520, 1119
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 3 cut(s) 459, 466, 1041
AflII CTTAAG 1 cut(s) 604
AhlI ACTAGT 1 cut(s) 113
AjiI CACGTC 1 cut(s) 866
AjnI CCWGG 3 cut(s) 473, 963, 1159
AluBI AGCT 5 cut(s) 83, 576, 663, 743, 1096
AluI AGCT 5 cut(s) 83, 576, 663, 743, 1096
Alw21I GWGCWC 3 cut(s) 101, 1083, 1098
Alw26I GTCTC 3 cut(s) 13, 219, 856
AlwI GGATC 3 cut(s) 144, 597, 1121
Ama87I CYCGRG 1 cut(s) 406
Aor13HI TCCGGA 1 cut(s) 466
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 3 cut(s) 308, 330, 436
ApeKI GCWGC 2 cut(s) 740, 828
ApoI RAATTY 1 cut(s) 1176
Asp700I GAANNNNTTC 2 cut(s) 846, 1119
AspLEI GCGC 3 cut(s) 167, 928, 1147
AspS9I GGNCC 1 cut(s) 615
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 1 cut(s) 791
AsuII TTCGAA 1 cut(s) 674
AsuNHI GCTAGC 1 cut(s) 1046
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 2 cut(s) 474, 1098
BarI GAAGNNNNNNTAC 2 cut(s) 901, 933
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 3 cut(s) 101, 1083, 1098
BbvI GCAGC 2 cut(s) 752, 840
BceAI ACGGC 1 cut(s) 423
BciT130I CCWGG 3 cut(s) 475, 965, 1161
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 3 cut(s) 13, 219, 856
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 6 cut(s) 114, 284, 339, 573, 1047, 1205
BfoI RGCGCY 2 cut(s) 168, 929
BfrI CTTAAG 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 478
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 3 cut(s) 330, 741, 829
BlpI GCTNAGC 1 cut(s) 1097
BlsI GCNGC 3 cut(s) 331, 742, 830
BmcAI AGTACT 1 cut(s) 1119
Bme1390I CCNGG 4 cut(s) 460, 475, 965, 1161
Bme18I GGWCC 1 cut(s) 615
BmeT110I CYCGRG 1 cut(s) 406
BmgBI CACGTC 1 cut(s) 866
BmgT120I GGNCC 1 cut(s) 615
BmiI GGNNCC 5 cut(s) 52, 389, 416, 471, 718
BmrFI CCNGG 4 cut(s) 460, 475, 965, 1161
BmtI GCTAGC 1 cut(s) 1050
BoxI GACNNNNGTC 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 690
BpmI CTGGAG 3 cut(s) 157, 735, 1046
Bpu1102I GCTNAGC 1 cut(s) 1097
Bpu14I TTCGAA 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 460
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 4 cut(s) 473, 474, 1035, 1084
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 3 cut(s) 459, 466, 1041
Bse118I RCCGGY 2 cut(s) 47, 310
Bse1I ACTGG 5 cut(s) 324, 391, 439, 618, 1029
BseAI TCCGGA 1 cut(s) 466
BseBI CCWGG 3 cut(s) 475, 965, 1161
BseDI CCNNGG 4 cut(s) 473, 474, 1035, 1084
BseLI CCNNNNNNNGG 3 cut(s) 459, 466, 1041
BseMII CTCAG 2 cut(s) 867, 1111
BseNI ACTGG 5 cut(s) 324, 391, 439, 618, 1029
BseRI GAGGAG 1 cut(s) 1091
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 2 cut(s) 752, 840
BsgI GTGCAG 1 cut(s) 999
Bsh1236I CGCG 1 cut(s) 361
Bsh1285I CGRYCG 3 cut(s) 333, 353, 385
BshFI GGCC 3 cut(s) 310, 332, 438
BshNI GGYRCC 1 cut(s) 50
BsiEI CGRYCG 3 cut(s) 333, 353, 385
BsiHKAI GWGCWC 3 cut(s) 101, 1083, 1098
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 5 cut(s) 48, 311, 460, 467, 923
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 459, 466, 1041
BsmAI GTCTC 3 cut(s) 13, 219, 856
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 3 cut(s) 310, 332, 438
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 4 cut(s) 101, 474, 1083, 1098
Bsp13I TCCGGA 1 cut(s) 466
Bsp143I GATC 5 cut(s) 149, 589, 851, 1063, 1126
Bsp1720I GCTNAGC 1 cut(s) 1097
BspACI CCGC 7 cut(s) 5, 45, 261, 281, 329, 361, 699
BspANI GGCC 3 cut(s) 310, 332, 438
BspCNI CTCAG 2 cut(s) 868, 1110
BspEI TCCGGA 1 cut(s) 466
BspFNI CGCG 1 cut(s) 361
BspLI GGNNCC 5 cut(s) 52, 389, 416, 471, 718
BspOI GCTAGC 1 cut(s) 1050
BspPI GGATC 3 cut(s) 144, 597, 1121
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 13
BsrFI RCCGGY 2 cut(s) 47, 310
BsrI ACTGG 5 cut(s) 324, 391, 439, 618, 1029
BssAI RCCGGY 2 cut(s) 47, 310
BssECI CCNNGG 4 cut(s) 473, 474, 1035, 1084
BssMI GATC 5 cut(s) 149, 589, 851, 1063, 1126
BssT1I CCWWGG 1 cut(s) 1035
Bst2UI CCWGG 3 cut(s) 475, 965, 1161
Bst4CI ACNGT 3 cut(s) 351, 847, 1085
Bst6I CTCTTC 3 cut(s) 242, 717, 1000
BstAFI CTTAAG 1 cut(s) 604
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 312, 665, 930, 1048
BstDEI CTNAG 2 cut(s) 876, 1097
BstDSI CCRYGG 1 cut(s) 1084
BstFNI CGCG 1 cut(s) 361
BstH2I RGCGCY 2 cut(s) 168, 929
BstHHI GCGC 3 cut(s) 167, 928, 1147
BstKTI GATC 5 cut(s) 152, 592, 854, 1066, 1129
BstMAI GTCTC 3 cut(s) 13, 219, 856
BstMBI GATC 5 cut(s) 149, 589, 851, 1063, 1126
BstMCI CGRYCG 3 cut(s) 333, 353, 385
BstMWI GCNNNNNNNGC 3 cut(s) 11, 478, 637
BstNI CCWGG 3 cut(s) 475, 965, 1161
BstNSI RCATGY 1 cut(s) 650
BstPAI GACNNNNGTC 1 cut(s) 408
BstSCI CCNGG 4 cut(s) 458, 473, 963, 1159
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 752, 840
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 3 cut(s) 149, 1063, 1126
BstXI CCANNNNNNTGG 1 cut(s) 1024
BstYI RGATCY 3 cut(s) 149, 1063, 1126
BstZI CGGCCG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 310, 332, 438
BtgI CCRYGG 1 cut(s) 1084
BtrI CACGTC 1 cut(s) 866
BtsIMutI CAGTG 2 cut(s) 331, 432
Cac8I GCNNGC 4 cut(s) 312, 665, 930, 1048
CfoI GCGC 3 cut(s) 167, 928, 1147
Cfr10I RCCGGY 2 cut(s) 47, 310
Cfr13I GGNCC 1 cut(s) 615
CsiI ACCWGGT 1 cut(s) 963
Csp6I GTAC 2 cut(s) 519, 1118
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 2 cut(s) 612, 647
CviQI GTAC 2 cut(s) 519, 1118
DdeI CTNAG 2 cut(s) 876, 1097
DpnI GATC 5 cut(s) 151, 591, 853, 1065, 1128
DpnII GATC 5 cut(s) 149, 589, 851, 1063, 1126
DraI TTTAAA 1 cut(s) 736
EaeI YGGCCR 2 cut(s) 330, 436
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 3 cut(s) 242, 717, 1000
EarI CTCTTC 3 cut(s) 242, 717, 1000
Ecl136II GAGCTC 1 cut(s) 1096
EclXI CGGCCG 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 1035
Eco24I GRGCYC 2 cut(s) 474, 1098
Eco31I GGTCTC 1 cut(s) 13
Eco32I GATATC 2 cut(s) 952, 1137
Eco47I GGWCC 1 cut(s) 615
Eco47III AGCGCT 1 cut(s) 166
Eco52I CGGCCG 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 2 cut(s) 876, 1130
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 1096
EcoRII CCWGG 3 cut(s) 473, 963, 1159
EcoRV GATATC 2 cut(s) 952, 1137
EcoT14I CCWWGG 1 cut(s) 1035
EcoT38I GRGCYC 2 cut(s) 474, 1098
ErhI CCWWGG 1 cut(s) 1035
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 2 cut(s) 615, 650
FaqI GGGAC 1 cut(s) 360
FatI CATG 2 cut(s) 611, 646
FauI CCCGC 2 cut(s) 52, 288
FblI GTMKAC 2 cut(s) 245, 429
Fnu4HI GCNGC 3 cut(s) 330, 741, 829
FriOI GRGCYC 2 cut(s) 474, 1098
Fsp4HI GCNGC 3 cut(s) 330, 741, 829
FspBI CTAG 6 cut(s) 114, 284, 339, 573, 1047, 1205
GlaI GCGC 3 cut(s) 166, 927, 1146
GluI GCNGC 3 cut(s) 330, 741, 829
GsuI CTGGAG 3 cut(s) 157, 735, 1046
HaeII RGCGCY 2 cut(s) 168, 929
HaeIII GGCC 3 cut(s) 310, 332, 438
HapII CCGG 5 cut(s) 48, 311, 460, 467, 923
HhaI GCGC 3 cut(s) 167, 928, 1147
Hin1II CATG 2 cut(s) 615, 650
Hin6I GCGC 3 cut(s) 165, 926, 1145
HinP1I GCGC 3 cut(s) 165, 926, 1145
HincII GTYRAC 3 cut(s) 268, 403, 430
HindII GTYRAC 3 cut(s) 268, 403, 430
HinfI GANTC 7 cut(s) 194, 251, 302, 404, 426, 880, 1104
HpaII CCGG 5 cut(s) 48, 311, 460, 467, 923
HphI GGTGA 1 cut(s) 791
Hpy166II GTNNAC 6 cut(s) 246, 268, 403, 430, 749, 1088
Hpy8I GTNNAC 6 cut(s) 246, 268, 403, 430, 749, 1088
Hpy99I CGWCG 1 cut(s) 444
HpyAV CCTTC 5 cut(s) 336, 539, 651, 903, 1105
HpyCH4III ACNGT 3 cut(s) 351, 847, 1085
HpyCH4IV ACGT 2 cut(s) 865, 942
HpyCH4V TGCA 3 cut(s) 650, 667, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 478, 637
HpyF3I CTNAG 2 cut(s) 876, 1097
HpySE526I ACGT 2 cut(s) 865, 942
Hsp92II CATG 2 cut(s) 615, 650
HspAI GCGC 3 cut(s) 165, 926, 1145
Kpn2I TCCGGA 1 cut(s) 466
KroI GCCGGC 1 cut(s) 310
KroNI GCCGGC 1 cut(s) 312
Kzo9I GATC 5 cut(s) 149, 589, 851, 1063, 1126
LmnI GCTCC 8 cut(s) 96, 387, 414, 455, 469, 716, 1027, 1078
Lsp1109I GCAGC 2 cut(s) 752, 840
MabI ACCWGGT 1 cut(s) 963
MaeI CTAG 6 cut(s) 114, 284, 339, 573, 1047, 1205
MaeII ACGT 2 cut(s) 865, 942
MalI GATC 5 cut(s) 151, 591, 853, 1065, 1128
MboI GATC 5 cut(s) 149, 589, 851, 1063, 1126
MflI RGATCY 3 cut(s) 149, 1063, 1126
MhlI GDGCHC 4 cut(s) 101, 474, 1083, 1098
MluCI AATT 6 cut(s) 676, 763, 885, 986, 1176, 1218
MlyI GAGTC 3 cut(s) 188, 398, 435
MmeI TCCRAC 3 cut(s) 365, 392, 567
MnlI CCTC 9 cut(s) 34, 64, 243, 300, 328, 535, 966, 1069, 1211
MroI TCCGGA 1 cut(s) 466
MroNI GCCGGC 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 846, 1119
MseI TTAA 4 cut(s) 605, 735, 1193, 1217
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 5 cut(s) 48, 311, 460, 467, 923
MspR9I CCNGG 4 cut(s) 460, 475, 965, 1161
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 3 cut(s) 475, 965, 1161
MvnI CGCG 1 cut(s) 361
MwoI GCNNNNNNNGC 3 cut(s) 11, 478, 637
NaeI GCCGGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 460
NdeII GATC 5 cut(s) 149, 589, 851, 1063, 1126
NgoMIV GCCGGC 1 cut(s) 310
NheI GCTAGC 1 cut(s) 1046
NlaIII CATG 2 cut(s) 615, 650
NlaIV GGNNCC 5 cut(s) 52, 389, 416, 471, 718
NspI RCATGY 1 cut(s) 650
NspV TTCGAA 1 cut(s) 674
PasI CCCWGGG 1 cut(s) 474
PctI GAATGC 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 312
PdmI GAANNNNTTC 2 cut(s) 846, 1119
PfeI GAWTC 4 cut(s) 251, 302, 880, 1104
PflFI GACNNNGTC 1 cut(s) 613
PfoI TCCNGGA 1 cut(s) 1159
PkrI GCNGC 3 cut(s) 331, 742, 830
PleI GAGTC 3 cut(s) 188, 398, 434
PpsI GAGTC 3 cut(s) 188, 398, 434
PshAI GACNNNNGTC 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 1098
Psp6I CCWGG 3 cut(s) 473, 963, 1159
PspGI CCWGG 3 cut(s) 473, 963, 1159
PspN4I GGNNCC 5 cut(s) 52, 389, 416, 471, 718
PspPI GGNCC 1 cut(s) 615
PsuI RGATCY 3 cut(s) 149, 1063, 1126
PsyI GACNNNGTC 1 cut(s) 613
RsaI GTAC 2 cut(s) 520, 1119
RsaNI GTAC 2 cut(s) 519, 1118
RseI CAYNNNNRTG 1 cut(s) 777
SacI GAGCTC 1 cut(s) 1098
SalI GTCGAC 1 cut(s) 428
SaqAI TTAA 4 cut(s) 605, 735, 1193, 1217
SatI GCNGC 3 cut(s) 330, 741, 829
Sau3AI GATC 5 cut(s) 149, 589, 851, 1063, 1126
Sau96I GGNCC 1 cut(s) 615
ScaI AGTACT 1 cut(s) 1119
SchI GAGTC 3 cut(s) 188, 398, 435
ScrFI CCNGG 4 cut(s) 460, 475, 965, 1161
SduI GDGCHC 4 cut(s) 101, 474, 1083, 1098
SexAI ACCWGGT 1 cut(s) 963
SfuI TTCGAA 1 cut(s) 674
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 2 cut(s) 198, 604
SmoI CTYRAG 2 cut(s) 198, 604
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 6 cut(s) 676, 763, 885, 986, 1176, 1218
SsiI CCGC 7 cut(s) 5, 45, 261, 281, 329, 361, 699
SspI AATATT 1 cut(s) 208
SspMI CTAG 6 cut(s) 114, 284, 339, 573, 1047, 1205
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 4 cut(s) 458, 473, 963, 1159
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 3 cut(s) 351, 847, 1085
TaiI ACGT 2 cut(s) 868, 945
TaqI TCGA 5 cut(s) 192, 305, 429, 674, 850
TaqII GACCGA 1 cut(s) 371
TasI AATT 6 cut(s) 676, 763, 885, 986, 1176, 1218
TatI WGTACW 2 cut(s) 518, 1117
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 4 cut(s) 251, 302, 880, 1104
Tru1I TTAA 4 cut(s) 605, 735, 1193, 1217
Tru9I TTAA 4 cut(s) 605, 735, 1193, 1217
TscAI CASTG 2 cut(s) 331, 439
TseI GCWGC 2 cut(s) 740, 828
TspDTI ATGAA 6 cut(s) 51, 522, 770, 776, 794, 855
TspGWI ACGGA 5 cut(s) 109, 263, 442, 475, 702
TspRI CASTG 2 cut(s) 331, 439
Tth111I GACNNNGTC 1 cut(s) 613
Vha464I CTTAAG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 615
XapI RAATTY 1 cut(s) 1176
XceI RCATGY 1 cut(s) 650
XmiI GTMKAC 2 cut(s) 245, 429
XmnI GAANNNNTTC 2 cut(s) 846, 1119
XspI CTAG 6 cut(s) 114, 284, 339, 573, 1047, 1205
ZrmI AGTACT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.