Prupe.2G270300_v2.0.a1

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012420
Physical Location & Seq
Forward (+)
27463336 .. 27466341
3006 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G270300.1

Sequence Viewer

Length: 1593 bp
ATGTCGTCGTCGTCGTCGTCGTCTCTCCCCAAAACCCCATACTCTCCCCCCTCCCCTCCTCCCTTAGACAGCTCCGACGCCGACCGCCGCCTCCGAGAGGCCGAGGACCGCCTCCGCGATGCCATCGAGGAGCTCCAGCGCCGCCAGCGCTCCGCCGCAGCTCGTGGGCCCCAGCACCTCCCGCCTTGCTACCACGCCAGCGACGAGTCGTGTATCGCCCACGCCATTGGCAATCTCTGCCAGAGCTTTCTCTTGTCGTATGGCGTACGAGTCGGAATTGGCATCCTTCTTCGCGCTTTCAAGCTCGCCCGCAGACAGTCGTACTCTTCACTCCTCGATCTCAAGCAACTAGTCTCAGAAAAAGATCTCATTGTAAGAGAAGAAGCATGCCGAATTGGTTTATTTTTTGGTGGCTTTAGTGGTTCTTATCATGCTCTTAGATGCTTGTTGAGAAAGTGGAGAAAGAAAGAGACACCATTTAATGCAATTTTAGCAGGTTCAGTTGCTGGTTTGTCTATTTTAGCGTTAAATGACTCAAACCGAAGGCGCACACTTTCTATGTACTTATTGGCCAGGCTAGCCCAGTGTGCTTATAATTCTGCGAAGTCTAAGAACAAGTTTCACTTTTGGGGAAGCCATTGGAGGCATGGGGATTCTCTACTTTTTTCAATTGCATGTGCACAGGTTATGTATGCTTTTGTAATGCGTCCTGAGAGCTTGCCAAAATCTTATCAAGAATTTATCCAGAAAACTGGGCCAGTTGCACAGCCTGTGTACAAGGCTATAAGGGAATGTTGTAGAGGTTACCCTGTGGATATTGCCTCACTATCTGATTACTTGTCTGAGAGAACGAAATCTGACTCTATAAAGTTGGAAGAGTATCCATCCATATTTCCTTGTTCTGTTATTCATCCAGACACAAATTCTTGTTTAGCTCACAATGCAATTGCAACATCAGCTACCTTTAGGAAAACGTTTCCACTTTACTTCTCTTTGACATTTGTACCATTCGTTGTTCTCCGCCTGCAGAAGTTCATGGAGGCCCCTGCTCGTACCTTTTTGGTTGCTCTTAAAGATGCTGTTCGCTCAACAACATTTTTGTCTGCTTTTGTTGGGATCTTTCAGGGTGTCATATGTTTGCATAGAAAAGTTGCATCAAAAGACCACAAGCTTCTATATTGGATTGCTGGTGGAATATCTGCTCTTTCAGTTTTACTGGAGAAAAAGGCTAGACGTGGTGAACTTGCTCTTTATGTTCTTCCGCGAGCAGGAGATTCTTTGTGGTATATCTTAGTAAATCGCCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTTTTGTTTTGTTTGTCTATGGGAGGAATCATGTACTACTTGGAACATGAGCCAGACACTATGGCTCCATTTCTCAGGGGTCTGATCCGTCGGTTCCTTGCTAGCAGAATAAGCAACCCTGTCTCTGCGTCCAATAGGAGTTCCTCCTACACATATTTGCAGAGTCTTGATGCCATGAAGAAGCCGAAGCTCTTAGATAGTCGTCGTACTGAATCACCTTCCGAGAAGTATAATCTTGAATCAATACCGGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

531

Amino Acids

59.59

Weight (kDa)

9.61

Isoelectric Point (pI)

61.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
AarI CACCTGC 1 cut(s) 1314
Acc36I ACCTGC 2 cut(s) 485, 1314
AccII CGCG 3 cut(s) 117, 294, 1264
AclI AACGTT 1 cut(s) 974
AclWI GGATC 2 cut(s) 1124, 1414
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 2 cut(s) 737, 922
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 8 cut(s) 267, 323, 563, 776, 1005, 1054, 1370, 1543
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 97, 1268
AgsI TTSAA 3 cut(s) 301, 669, 1574
AhlI ACTAGT 1 cut(s) 349
AjiI CACGTC 1 cut(s) 1235
AjnI CCWGG 1 cut(s) 572
Alw21I GWGCWC 2 cut(s) 135, 682
Alw26I GTCTC 4 cut(s) 27, 358, 464, 1462
Alw44I GTGCAC 1 cut(s) 678
AlwI GGATC 2 cut(s) 1124, 1414
AlwNI CAGNNNCTG 1 cut(s) 506
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 5 cut(s) 99, 167, 570, 755, 1041
ApaI GGGCCC 1 cut(s) 171
ApaLI GTGCAC 1 cut(s) 678
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 737, 922
AspLEI GCGC 4 cut(s) 141, 150, 296, 549
AspS9I GGNCC 5 cut(s) 106, 167, 168, 755, 1042
AsuC2I CCSGG 1 cut(s) 1584
AsuHPI GGTGA 2 cut(s) 1250, 1542
AsuNHI GCTAGC 2 cut(s) 577, 1436
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 2 cut(s) 171, 682
BaeI ACNNNNGTAYC 2 cut(s) 987, 1020
BalI TGGCCA 1 cut(s) 572
BanII GRGCYC 2 cut(s) 135, 171
BauI CACGAG 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 135, 682
BbvCI CCTCAGC 1 cut(s) 1329
BbvI GCAGC 1 cut(s) 170
BccI CCATC 2 cut(s) 131, 892
BciT130I CCWGG 1 cut(s) 574
BciVI GTATCC 1 cut(s) 891
BcnI CCSGG 1 cut(s) 1584
BcoDI GTCTC 4 cut(s) 27, 358, 464, 1462
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 4 cut(s) 350, 578, 1230, 1437
BfmI CTRYAG 1 cut(s) 1025
BfoI RGCGCY 2 cut(s) 142, 151
BfuAI ACCTGC 2 cut(s) 485, 1314
BfuI GTATCC 1 cut(s) 891
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 88, 142, 156, 159
BlsI GCNGC 4 cut(s) 89, 143, 157, 160
Bme1390I CCNGG 2 cut(s) 574, 1584
Bme18I GGWCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 1235
BmgT120I GGNCC 5 cut(s) 106, 167, 168, 755, 1042
BmiI GGNNCC 5 cut(s) 169, 170, 1044, 1401, 1430
BmrFI CCNGG 2 cut(s) 574, 1584
BmrI ACTGGG 2 cut(s) 577, 762
BmsI GCATC 6 cut(s) 109, 291, 431, 1066, 1163, 1495
BmtI GCTAGC 2 cut(s) 581, 1440
BmuI ACTGGG 2 cut(s) 577, 762
BpmI CTGGAG 2 cut(s) 119, 1238
Bpu10I CCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 1584
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 102, 1583
BsaXI ACNNNNNCTCC 2 cut(s) 1384, 1414
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 1268
Bse1I ACTGG 4 cut(s) 583, 757, 758, 1221
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 2 cut(s) 102, 1583
BseGI GGATG 3 cut(s) 282, 884, 910
BseLI CCNNNNNNNGG 2 cut(s) 97, 1268
BseMII CTCAG 5 cut(s) 369, 702, 834, 1320, 1423
BseNI ACTGG 4 cut(s) 583, 757, 758, 1221
BseRI GAGGAG 3 cut(s) 48, 143, 323
BseSI GKGCMC 2 cut(s) 171, 682
BseXI GCAGC 1 cut(s) 170
BseYI CCCAGC 1 cut(s) 171
Bsh1236I CGCG 3 cut(s) 117, 294, 1264
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 5 cut(s) 101, 169, 572, 757, 1043
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 2 cut(s) 135, 682
BsiSI CCGG 1 cut(s) 1583
BsiWI CGTACG 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 97, 1268
BsmAI GTCTC 4 cut(s) 27, 358, 464, 1462
BsmBI CGTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 1330
BsnI GGCC 5 cut(s) 101, 169, 572, 757, 1043
Bsp120I GGGCCC 1 cut(s) 167
Bsp1286I GDGCHC 3 cut(s) 135, 171, 682
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 4 cut(s) 337, 364, 1116, 1419
BspANI GGCC 5 cut(s) 101, 169, 572, 757, 1043
BspCNI CTCAG 5 cut(s) 368, 703, 835, 1321, 1422
BspFNI CGCG 3 cut(s) 117, 294, 1264
BspLI GGNNCC 5 cut(s) 169, 170, 1044, 1401, 1430
BspMAI CTGCAG 1 cut(s) 1029
BspMI ACCTGC 2 cut(s) 485, 1314
BspOI GCTAGC 2 cut(s) 581, 1440
BspPI GGATC 2 cut(s) 1124, 1414
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 4 cut(s) 583, 757, 758, 1221
BssECI CCNNGG 2 cut(s) 102, 1583
BssMI GATC 4 cut(s) 337, 364, 1116, 1419
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 331, 870
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 237
BstAUI TGTACA 1 cut(s) 774
BstEII GGTNACC 1 cut(s) 803
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 3 cut(s) 282, 884, 910
BstFNI CGCG 3 cut(s) 117, 294, 1264
BstH2I RGCGCY 2 cut(s) 142, 151
BstHHI GCGC 4 cut(s) 141, 150, 296, 549
BstKTI GATC 4 cut(s) 340, 367, 1119, 1422
BstMAI GTCTC 4 cut(s) 27, 358, 464, 1462
BstMBI GATC 4 cut(s) 337, 364, 1116, 1419
BstMCI CGRYCG 1 cut(s) 85
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 2 cut(s) 390, 678
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 572, 1582
BstSFI CTRYAG 1 cut(s) 1025
BstSLI GKGCMC 2 cut(s) 171, 682
BstUI CGCG 3 cut(s) 117, 294, 1264
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 2 cut(s) 364, 1116
BstXI CCANNNNNNTGG 2 cut(s) 227, 752
BstYI RGATCY 2 cut(s) 364, 1116
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 5 cut(s) 101, 169, 572, 757, 1043
BtgZI GCGATG 1 cut(s) 132
BtrI CACGTC 1 cut(s) 1235
BtsCI GGATG 3 cut(s) 282, 884, 910
BtsIMutI CAGTG 1 cut(s) 590
BveI ACCTGC 2 cut(s) 485, 1314
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 4 cut(s) 141, 150, 296, 549
Cfr13I GGNCC 5 cut(s) 106, 167, 168, 755, 1042
CseI GACGC 3 cut(s) 86, 695, 1452
Csp6I GTAC 8 cut(s) 266, 322, 562, 775, 1004, 1053, 1369, 1542
CviAII CATG 8 cut(s) 387, 431, 647, 675, 1036, 1366, 1382, 1510
CviQI GTAC 8 cut(s) 266, 322, 562, 775, 1004, 1053, 1369, 1542
DpnI GATC 4 cut(s) 339, 366, 1118, 1421
DpnII GATC 4 cut(s) 337, 364, 1116, 1419
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 2 cut(s) 331, 870
EarI CTCTTC 2 cut(s) 331, 870
EciI GGCGGA 2 cut(s) 142, 1010
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 2 cut(s) 135, 171
Eco47I GGWCC 1 cut(s) 106
Eco47III AGCGCT 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoO109I RGGNCCY 2 cut(s) 168, 1042
EcoO65I GGTNACC 1 cut(s) 803
EcoRII CCWGG 1 cut(s) 572
EcoT38I GRGCYC 2 cut(s) 135, 171
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 8 cut(s) 390, 434, 650, 678, 1039, 1369, 1385, 1513
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FatI CATG 8 cut(s) 386, 430, 646, 674, 1035, 1365, 1381, 1509
FauI CCCGC 2 cut(s) 189, 317
FauNDI CATATG 1 cut(s) 1133
Fnu4HI GCNGC 4 cut(s) 88, 142, 156, 159
FokI GGATG 3 cut(s) 269, 871, 897
FriOI GRGCYC 2 cut(s) 135, 171
Fsp4HI GCNGC 4 cut(s) 88, 142, 156, 159
FspBI CTAG 4 cut(s) 350, 578, 1230, 1437
GlaI GCGC 4 cut(s) 140, 149, 295, 548
GluI GCNGC 4 cut(s) 88, 142, 156, 159
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 2 cut(s) 119, 1238
HaeII RGCGCY 2 cut(s) 142, 151
HaeIII GGCC 5 cut(s) 101, 169, 572, 757, 1043
HapII CCGG 1 cut(s) 1583
HgaI GACGC 3 cut(s) 86, 695, 1452
HhaI GCGC 4 cut(s) 141, 150, 296, 549
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 8 cut(s) 390, 434, 650, 678, 1039, 1369, 1385, 1513
Hin6I GCGC 4 cut(s) 139, 148, 294, 547
HinP1I GCGC 4 cut(s) 139, 148, 294, 547
HindIII AAGCTT 1 cut(s) 1169
HpaII CCGG 1 cut(s) 1583
HphI GGTGA 2 cut(s) 1250, 1542
Hpy166II GTNNAC 3 cut(s) 680, 775, 1241
Hpy188I TCNGA 9 cut(s) 76, 95, 275, 358, 832, 844, 859, 1419, 1558
Hpy188III TCNNGA 7 cut(s) 710, 734, 745, 914, 1313, 1502, 1571
Hpy8I GTNNAC 3 cut(s) 680, 775, 1241
Hpy99I CGWCG 9 cut(s) 10, 13, 16, 19, 22, 80, 206, 1428, 1542
HpyAV CCTTC 3 cut(s) 296, 537, 1563
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 2 cut(s) 974, 1234
HpySE526I ACGT 2 cut(s) 974, 1234
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 8 cut(s) 390, 434, 650, 678, 1039, 1369, 1385, 1513
HspAI GCGC 4 cut(s) 139, 148, 294, 547
Kzo9I GATC 4 cut(s) 337, 364, 1116, 1419
LmnI GCTCC 5 cut(s) 77, 130, 138, 155, 1405
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 6 cut(s) 109, 291, 431, 1066, 1163, 1495
MaeI CTAG 4 cut(s) 350, 578, 1230, 1437
MaeII ACGT 2 cut(s) 974, 1234
MaeIII GTNAC 1 cut(s) 803
MalI GATC 4 cut(s) 339, 366, 1118, 1421
MboI GATC 4 cut(s) 337, 364, 1116, 1419
MboII GAAGA 6 cut(s) 281, 318, 392, 887, 1250, 1525
MfeI CAATTG 2 cut(s) 669, 945
MflI RGATCY 2 cut(s) 364, 1116
MhlI GDGCHC 3 cut(s) 135, 171, 682
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 8 cut(s) 276, 393, 486, 595, 669, 737, 922, 945
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 5 cut(s) 215, 279, 527, 854, 1507
MmeI TCCRAC 3 cut(s) 99, 253, 852
Mox20I TGGCCA 1 cut(s) 572
MscI TGGCCA 1 cut(s) 572
MseI TTAA 4 cut(s) 480, 527, 1071, 1320
Msp20I TGGCCA 1 cut(s) 572
MspI CCGG 1 cut(s) 1583
MspR9I CCNGG 2 cut(s) 574, 1584
MunI CAATTG 2 cut(s) 669, 945
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 3 cut(s) 117, 294, 1264
NciI CCSGG 1 cut(s) 1584
NdeI CATATG 1 cut(s) 1133
NdeII GATC 4 cut(s) 337, 364, 1116, 1419
NheI GCTAGC 2 cut(s) 577, 1436
NlaIII CATG 8 cut(s) 390, 434, 650, 678, 1039, 1369, 1385, 1513
NlaIV GGNNCC 5 cut(s) 169, 170, 1044, 1401, 1430
NmeAIII GCCGAG 1 cut(s) 127
NspI RCATGY 2 cut(s) 390, 678
PaeI GCATGC 1 cut(s) 390
PaqCI CACCTGC 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 4 cut(s) 11, 14, 17, 201
PctI GAATGC 1 cut(s) 1330
PfeI GAWTC 5 cut(s) 653, 1274, 1362, 1547, 1574
Pfl23II CGTACG 1 cut(s) 265
PkrI GCNGC 4 cut(s) 89, 143, 157, 160
PleI GAGTC 5 cut(s) 214, 278, 527, 854, 1506
PpsI GAGTC 5 cut(s) 214, 278, 527, 854, 1506
PsiI TTATAA 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 974
Psp6I CCWGG 1 cut(s) 572
PspEI GGTNACC 1 cut(s) 803
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 572
PspLI CGTACG 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 169, 170, 1044, 1401, 1430
PspOMI GGGCCC 1 cut(s) 167
PspPI GGNCC 5 cut(s) 106, 167, 168, 755, 1042
PstI CTGCAG 1 cut(s) 1029
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 2 cut(s) 364, 1116
RsaI GTAC 8 cut(s) 267, 323, 563, 776, 1005, 1054, 1370, 1543
RsaNI GTAC 8 cut(s) 266, 322, 562, 775, 1004, 1053, 1369, 1542
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 4 cut(s) 480, 527, 1071, 1320
SatI GCNGC 4 cut(s) 88, 142, 156, 159
Sau3AI GATC 4 cut(s) 337, 364, 1116, 1419
Sau96I GGNCC 5 cut(s) 106, 167, 168, 755, 1042
SchI GAGTC 5 cut(s) 215, 279, 527, 854, 1507
ScrFI CCNGG 2 cut(s) 574, 1584
SduI GDGCHC 3 cut(s) 135, 171, 682
SfaNI GCATC 6 cut(s) 109, 291, 431, 1066, 1163, 1495
SfcI CTRYAG 1 cut(s) 1025
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 349
SphI GCATGC 1 cut(s) 390
Sse9I AATT 8 cut(s) 276, 393, 486, 595, 669, 737, 922, 945
SspMI CTAG 4 cut(s) 350, 578, 1230, 1437
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 572, 1582
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 2 cut(s) 977, 1237
TaqI TCGA 2 cut(s) 126, 336
TasI AATT 8 cut(s) 276, 393, 486, 595, 669, 737, 922, 945
TatI WGTACW 3 cut(s) 561, 774, 1368
TauI GCSGC 3 cut(s) 90, 144, 158
TfiI GAWTC 5 cut(s) 653, 1274, 1362, 1547, 1574
Tru1I TTAA 4 cut(s) 480, 527, 1071, 1320
Tru9I TTAA 4 cut(s) 480, 527, 1071, 1320
TscAI CASTG 1 cut(s) 590
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 3 cut(s) 899, 1024, 1526
TspGWI ACGGA 1 cut(s) 1412
TspRI CASTG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 678
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 2 cut(s) 737, 922
XceI RCATGY 2 cut(s) 390, 678
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XspI CTAG 4 cut(s) 350, 578, 1230, 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.