RLG00000026833

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012420
Physical Location & Seq
Reverse (-)
6355242 .. 6358142
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026833

Sequence Viewer

Length: 1260 bp
ATGGCGTCCGAGTTGGGATTGGGATTCTTCTTCGCGCGTTTAAGCTCGCTCGCAGGCAATCCTACTCTTCCTTGCTCGATCTTAAGTGGCTCTTACCATGCTATTAGATGCTTGTTGAGAAAGTACAGAAAGAAAGAGACACCATTGAATGCAATCTTAGCAGGTTCAGTTGCGGGGTTGTCTATTTTAGCATTAGATGACTCAAACCGAAGGCGCACGCTATCTCTGTACTTGTTGGCAAGGGTAGCACAGTCTGCTTATAATTCTGCAAAGTCTAAGAACAAGTTTCACTTTTGGGGAAGCCACTGGAGGCATGGGGATTCTCTGCTCTTTTCTATTGCATGTGCACAGGTTATGTACGCCTTTGTAATGCGTCCTGAGAGCTTGCCTAAATCTTATCATGAATTTATCCAGAAGACTGGTCCTGTTGCTCAGCCTGTATACAAGGCCGTAAGGGAGTGTTGTAGAGGATACCCAGTGGATATTGCCTCACTGGCTGATTACTTGGCCGAGAAAGGGAGAAGCGATATAAAGTTGGAAGAGTTTCCATCCATTTTGCCTTGTTCTGTTATTCATCCCCACACAAATTCTTGTTTAGTGCACAATGCAACTGCAACATCAGCTACATTTAGGAAAACATTTCCGCTCTACTTCTCTTTGACGTTTGTACCATTTGTTGTTCTTCGCCTACAGAAGTTCCTGGCGGCCCCTGCTCGCACCTTTATGTTTGCTGTTAAAGATTCTGTTCGCTCAACAGCATTCTTGTCTGCTTTCGTCGGAATCTTTCAGGGTGTGATATGTTTGCACAGAAAAGTTGCATCAAAAGACAACAAGCTTGTATATTGGATTGCTGGTGGTCTATCTGCTCTTTCAGTTCTACTGGAGAAAAAGGCTAGGCGTGGTGAACTTGCTCTATATGTTCTTCCACGGGCAGGAGATTCTTTGTGGTATATTTTAGTAAACCGTCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTCTTGTTCTGTTTGTGTATGGGAGGAATTATGTACTACTTGGAACATGAGCCAGACACTATGGCTCCATTCCTGAGGGGTCTGATTCGCCGCTTTCTTGCTAGCCGAATTAGTAATCCGGTCAATTCATCAAATAGGAATGCTTCCTACACATATTTGCAAACTCTTGATGCCATGAAGAAGCCGAAGCTATTAGAAAATGATGGTTCTGAAGCACCTTCTGAGAAGAAGTACAATCTTGAATCAATACCAGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

420

Amino Acids

46.83

Weight (kDa)

9.63

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM135_C_rich PF15982 190 - 318 9.7e-09 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AarI CACCTGC 1 cut(s) 978
Acc36I ACCTGC 2 cut(s) 152, 978
AccBSI CCGCTC 1 cut(s) 646
AccI GTMKAC 1 cut(s) 441
AccII CGCG 2 cut(s) 35, 37
AciI CCGC 4 cut(s) 173, 644, 704, 1090
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 2 cut(s) 404, 586
AcuI CTGAAG 1 cut(s) 1230
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 125, 230, 359, 669, 1034, 1232
AfiI CCNNNNNNNGG 2 cut(s) 516, 932
AflII CTTAAG 1 cut(s) 82
AgsI TTSAA 2 cut(s) 148, 1241
AjnI CCWGG 2 cut(s) 699, 1249
AluBI AGCT 5 cut(s) 45, 384, 623, 835, 1189
AluI AGCT 5 cut(s) 45, 384, 623, 835, 1189
Alw21I GWGCWC 2 cut(s) 349, 603
Alw26I GTCTC 1 cut(s) 131
Alw44I GTGCAC 2 cut(s) 345, 599
AoxI GGCC 3 cut(s) 447, 507, 705
ApaLI GTGCAC 2 cut(s) 345, 599
ApoI RAATTY 2 cut(s) 404, 586
Asp700I GAANNNNTTC 1 cut(s) 543
AspLEI GCGC 2 cut(s) 37, 216
AspS9I GGNCC 2 cut(s) 422, 706
AsuHPI GGTGA 2 cut(s) 914, 959
AsuNHI GCTAGC 1 cut(s) 1100
AvaII GGWCC 1 cut(s) 422
AxyI CCTNAGG 1 cut(s) 1073
BaeGI GKGCMC 2 cut(s) 349, 603
BaeI ACNNNNGTAYC 2 cut(s) 651, 684
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 2 cut(s) 349, 603
BbvCI CCTCAGC 1 cut(s) 993
BccI CCATC 2 cut(s) 556, 1196
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 2 cut(s) 701, 1251
BciVI GTATCC 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 894, 1101
BfmI CTRYAG 1 cut(s) 689
BfrI CTTAAG 1 cut(s) 82
BfuAI ACCTGC 2 cut(s) 152, 978
BfuI GTATCC 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 494
BisI GCNGC 2 cut(s) 705, 1090
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 2 cut(s) 706, 1091
Bme1390I CCNGG 2 cut(s) 701, 1251
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 2 cut(s) 422, 706
BmiI GGNNCC 2 cut(s) 708, 1065
BmrFI CCNGG 2 cut(s) 701, 1251
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 3 cut(s) 98, 827, 1159
BmtI GCTAGC 1 cut(s) 1104
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 422
BpmI CTGGAG 2 cut(s) 328, 902
Bpu10I CCTNAGC 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 926, 1250
BsaWI WCCGGW 1 cut(s) 1117
BsaXI ACNNNNNCTCC 2 cut(s) 1048, 1078
Bsc4I CCNNNNNNNGG 2 cut(s) 516, 932
Bse1I ACTGG 5 cut(s) 311, 424, 476, 498, 885
Bse21I CCTNAGG 1 cut(s) 1073
BseBI CCWGG 2 cut(s) 701, 1251
BseDI CCNNGG 2 cut(s) 926, 1250
BseGI GGATG 2 cut(s) 548, 574
BseLI CCNNNNNNNGG 2 cut(s) 516, 932
BseMII CTCAG 5 cut(s) 369, 446, 984, 1064, 1212
BseNI ACTGG 5 cut(s) 311, 424, 476, 498, 885
BseSI GKGCMC 2 cut(s) 349, 603
Bsh1236I CGCG 2 cut(s) 35, 37
BshFI GGCC 3 cut(s) 449, 509, 707
BsiHKAI GWGCWC 2 cut(s) 349, 603
BsiSI CCGG 1 cut(s) 1118
BslI CCNNNNNNNGG 2 cut(s) 516, 932
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 4 cut(s) 154, 758, 994, 1144
BsnI GGCC 3 cut(s) 449, 509, 707
Bsp1286I GDGCHC 2 cut(s) 349, 603
Bsp143I GATC 1 cut(s) 78
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 4 cut(s) 173, 644, 704, 1090
BspANI GGCC 3 cut(s) 449, 509, 707
BspCNI CTCAG 5 cut(s) 370, 445, 985, 1065, 1213
BspFNI CGCG 2 cut(s) 35, 37
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 708, 1065
BspMI ACCTGC 2 cut(s) 152, 978
BspOI GCTAGC 1 cut(s) 1104
BspTI CTTAAG 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 646
BsrI ACTGG 5 cut(s) 311, 424, 476, 498, 885
BssECI CCNNGG 2 cut(s) 926, 1250
BssMI GATC 1 cut(s) 78
BssNAI GTATAC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 701, 1251
Bst4CI ACNGT 2 cut(s) 252, 965
Bst6I CTCTTC 2 cut(s) 72, 534
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 254
BstC8I GCNNGC 7 cut(s) 47, 51, 55, 218, 386, 715, 1102
BstDEI CTNAG 7 cut(s) 157, 276, 378, 432, 993, 1073, 1221
BstDSI CCRYGG 1 cut(s) 926
BstF5I GGATG 2 cut(s) 548, 574
BstFNI CGCG 2 cut(s) 35, 37
BstHHI GCGC 2 cut(s) 37, 216
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 6 cut(s) 158, 245, 254, 494, 620, 710
BstNI CCWGG 2 cut(s) 701, 1251
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 699, 1249
BstSFI CTRYAG 1 cut(s) 689
BstSLI GKGCMC 2 cut(s) 349, 603
BstUI CGCG 2 cut(s) 35, 37
BstV2I GAAGAC 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 442
Bsu36I CCTNAGG 1 cut(s) 1073
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 3 cut(s) 449, 509, 707
BtgI CCRYGG 1 cut(s) 926
BtsCI GGATG 2 cut(s) 548, 574
BtsIMutI CAGTG 3 cut(s) 304, 483, 491
BveI ACCTGC 2 cut(s) 152, 978
Cac8I GCNNGC 7 cut(s) 47, 51, 55, 218, 386, 715, 1102
CciI TCATGA 1 cut(s) 400
CfoI GCGC 2 cut(s) 37, 216
Cfr13I GGNCC 2 cut(s) 422, 706
CseI GACGC 1 cut(s) 362
Csp6I GTAC 6 cut(s) 124, 229, 358, 668, 1033, 1231
CviAII CATG 6 cut(s) 98, 314, 342, 401, 1046, 1174
CviQI GTAC 6 cut(s) 124, 229, 358, 668, 1033, 1231
DdeI CTNAG 7 cut(s) 157, 276, 378, 432, 993, 1073, 1221
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 72, 534
EarI CTCTTC 2 cut(s) 72, 534
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 1230
Eco81I CCTNAGG 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 699, 1249
FaeI CATG 6 cut(s) 101, 317, 345, 404, 1049, 1177
FalI AAGNNNNNCTT 4 cut(s) 76, 108, 275, 307
FatI CATG 6 cut(s) 97, 313, 341, 400, 1045, 1173
FauI CCCGC 1 cut(s) 166
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 705, 1090
FokI GGATG 2 cut(s) 535, 561
Fsp4HI GCNGC 2 cut(s) 705, 1090
FspBI CTAG 2 cut(s) 894, 1101
GlaI GCGC 2 cut(s) 36, 215
GluI GCNGC 2 cut(s) 705, 1090
GsuI CTGGAG 2 cut(s) 328, 902
HaeIII GGCC 3 cut(s) 449, 509, 707
HapII CCGG 1 cut(s) 1118
HgaI GACGC 1 cut(s) 362
HhaI GCGC 2 cut(s) 37, 216
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 101, 317, 345, 404, 1049, 1177
Hin6I GCGC 2 cut(s) 35, 214
HinP1I GCGC 2 cut(s) 35, 214
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 8 cut(s) 24, 200, 320, 740, 780, 938, 1084, 1241
HpaII CCGG 1 cut(s) 1118
HphI GGTGA 2 cut(s) 914, 959
Hpy166II GTNNAC 5 cut(s) 347, 442, 601, 905, 961
Hpy188I TCNGA 5 cut(s) 10, 779, 1083, 1210, 1222
Hpy188III TCNNGA 7 cut(s) 377, 401, 412, 977, 1072, 1166, 1238
Hpy8I GTNNAC 5 cut(s) 347, 442, 601, 905, 961
Hpy99I CGWCG 1 cut(s) 779
HpyAV CCTTC 2 cut(s) 204, 1227
HpyCH4III ACNGT 2 cut(s) 252, 965
HpyCH4IV ACGT 1 cut(s) 662
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 245, 254, 494, 620, 710
HpyF3I CTNAG 7 cut(s) 157, 276, 378, 432, 993, 1073, 1221
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 101, 317, 345, 404, 1049, 1177
HspAI GCGC 2 cut(s) 35, 214
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 1069
LweI GCATC 3 cut(s) 98, 827, 1159
MaeI CTAG 2 cut(s) 894, 1101
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 1 cut(s) 965
MalI GATC 1 cut(s) 80
MbiI CCGCTC 1 cut(s) 646
MboI GATC 1 cut(s) 78
MboII GAAGA 9 cut(s) 19, 22, 59, 427, 551, 674, 914, 1189, 1237
MhlI GDGCHC 2 cut(s) 349, 603
MluCI AATT 6 cut(s) 262, 404, 586, 1026, 1107, 1123
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 516, 757
MnlI CCTC 6 cut(s) 303, 461, 499, 988, 1016, 1068
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 4 cut(s) 41, 83, 735, 984
MslI CAYNNNNRTG 1 cut(s) 722
MspCI CTTAAG 1 cut(s) 82
MspI CCGG 1 cut(s) 1118
MspR9I CCNGG 2 cut(s) 701, 1251
Mva1269I GAATGC 4 cut(s) 154, 758, 994, 1144
MvaI CCWGG 2 cut(s) 701, 1251
MvnI CGCG 2 cut(s) 35, 37
MwoI GCNNNNNNNGC 6 cut(s) 158, 245, 254, 494, 620, 710
NdeII GATC 1 cut(s) 78
NheI GCTAGC 1 cut(s) 1100
NlaIII CATG 6 cut(s) 101, 317, 345, 404, 1049, 1177
NlaIV GGNNCC 2 cut(s) 708, 1065
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 965
NspI RCATGY 1 cut(s) 345
PagI TCATGA 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 978
PctI GAATGC 4 cut(s) 154, 758, 994, 1144
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 7 cut(s) 24, 320, 740, 780, 938, 1084, 1241
PkrI GCNGC 2 cut(s) 706, 1091
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 699, 1249
PspGI CCWGG 2 cut(s) 699, 1249
PspN4I GGNNCC 2 cut(s) 708, 1065
PspPI GGNCC 2 cut(s) 422, 706
RsaI GTAC 6 cut(s) 125, 230, 359, 669, 1034, 1232
RsaNI GTAC 6 cut(s) 124, 229, 358, 668, 1033, 1231
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 4 cut(s) 41, 83, 735, 984
SatI GCNGC 2 cut(s) 705, 1090
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 2 cut(s) 422, 706
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 701, 1251
SduI GDGCHC 2 cut(s) 349, 603
SfaNI GCATC 3 cut(s) 98, 827, 1159
SfcI CTRYAG 1 cut(s) 689
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 6 cut(s) 262, 404, 586, 1026, 1107, 1123
SsiI CCGC 4 cut(s) 173, 644, 704, 1090
SspMI CTAG 2 cut(s) 894, 1101
StyD4I CCNGG 2 cut(s) 699, 1249
TaaI ACNGT 2 cut(s) 252, 965
TaiI ACGT 1 cut(s) 665
TaqI TCGA 1 cut(s) 77
TasI AATT 6 cut(s) 262, 404, 586, 1026, 1107, 1123
TatI WGTACW 4 cut(s) 123, 228, 1032, 1230
TauI GCSGC 2 cut(s) 707, 1092
TfiI GAWTC 7 cut(s) 24, 320, 740, 780, 938, 1084, 1241
Tru1I TTAA 4 cut(s) 41, 83, 735, 984
Tru9I TTAA 4 cut(s) 41, 83, 735, 984
TscAI CASTG 3 cut(s) 311, 483, 498
TseFI GTSAC 1 cut(s) 965
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 4 cut(s) 417, 563, 1116, 1190
TspRI CASTG 3 cut(s) 311, 483, 498
Vha464I CTTAAG 1 cut(s) 82
VneI GTGCAC 2 cut(s) 345, 599
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 2 cut(s) 404, 586
XceI RCATGY 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XmiI GTMKAC 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 2 cut(s) 894, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.