Rroxscaffold_4G00284300

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012420
Physical Location & Seq
Reverse (-)
5933488 .. 5941432
7945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284300.1

Sequence Viewer

Length: 3858 bp
ATGCCGTCGCCGTCGTCTCTCCCCAAACCCCCGACCTCCCCGCCGCCGCCCCTAGACGCCTCCGACCCCCTCCGCGAGGCCGAGGACCGCCTCCGCGACGCCATTGAGGAGCTCCAGCGCCGCCAGCGCACCGCCGCCGCCCGCAGCTCCCAGCACCACCCCCTGCGACCACGCCAACGACGAGTCTTGCGTCGCCCACGCCATTGGGAACCTCTGCCAGAGCTTTCTCCTCTCCTATGGCGTCCGAGTTGGGATTGGGATTCTTCTTCGCGCCAACTTGTCTCGGAAAAAGATTTGATTGTGAGAGAAGAAGCATGCCGAATTGGCTTATTTTTTGGTGGATTTAGTGGCTCTTACCATGCTATTAGATGCTTGTTGAGAAAGTACAGAAAGAAAGAGACACCATTGAATGCAATCTTAGCAGGTTCAATTGCGGGGTTGTCTATTTTAGCATTAGATGACTCAAACCGAAGGCGGACACTATCTCTCTACTTGTTGGCAAGGGTAGCACAGTCTGCTTATAATTCTGCAAAGTCTAAGAACAAGTTTCACTTTTGGGGAAGCCACTGGAGGCATGGGGATTCTCTGCTCTTTTCTATTGCATGTGCACAGGTTATGTACGCCTTTGTAATGCGTCCTGAGAGCTTGCCTAAATCTTATCATGAATTTATCCAGAAGACTGGTCCTGTTGCTCAGCCTGTATACAAGGCCGTAAGGGAGTGTTGTAGAGGATACCCAGTGGATATTGCCTCACTGGCTGATTACTTGGCTGAGAAAGGGAGAAGCGATATAAAGTTGGAAGAGTTTCCATCCATTTTGCCTTGTTCTGTTATTCATCCCCACACAAATTCTTGTTTAGTGCACAATGCAACTGCAACATCAGCTACATTTAGGAAAACATTTCCGCTTTACTTCTCTTTGACGTTTGTACCATTTGTTGTTCTTCGCCTACAGAAGTTCCTGGCGGCCCCTGCTCGCACCTTTATGTTTGCTGTTAAAGATTCTGTTCGCTCAACAGCATTCTTATCTGCTTTCGTCGGAATCTTTCAGGGTGTGATATGTTTGCACAGAAAAGTTGCATCGAAAGACAACAAGCTTGTATATTGGATTGCTGGTGGTCTATCTGCTCTTTCAGTTCTACTGGAGAAAAAGGCTAGGCGTGGTGAACTTGCTCTATATGTTCTTCCACGGGCAGGAGATTCTTTGTGGTATATTTTAGTAAACCGTCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTCTTGTTCTGTTTGTGTATGGGAGGAATTATGTACTACTTGGAACATGAGCCAGACACTATGGCTCCATTCCTGAGGGGTCTGATTCGCCGCTTTCTTGCTAGCCGAATTAGTAATCCGGTCAATTCGTCTAATAGGAATGCTTCCTACACATATTTGCAAACTCTTGATGCCATGAAGAAGCCGAAGCTATTAGAAAATGATGGTTCTGAAGCACCTTCTGAGAAGAAGTACAATCTTGAATCAATACCAGGGCTGCTGCTTGAGGCTTCTCAAACTGGAAATGTCCAGCTTTTGCATCAATTGCAGAGAGACGATCCACAGCTTCTGCATAATGTTTTGCTAGCTTCCGGAGAGAATCCCTTGTATGTTGCTTCCGCTGCTGGTCATGTGGAATTTGTCGAGGTGATTCTAGAGTTGAAACCAGCTTTGGCCAAAGTATTCAACAAGGATGGCTTTAGCCCCATGGATGTGGCATCTGCCAATGGCTATCTGGAGATTGTAAGGGCTCTGCAGAAAGCTTTACCTGAACTCTCCCAGGATAAAGGAAAAGATCATTGGACTCCACTGCATCATGCTGCTAGAAGAGGGAGGGTAGATGTTATCCGAGAAATAGTTTCGGCTTGTCCAGAAAGCGTTGAAGACGTGACAAGACAAGGGGAGACTGCGTTACACCTTGCTGTTAAGAACAGCCAATTTGAAGCAATAAGAGTCCTGTTGGAATTGGTGCGAGAAATGGACAAGCTGCATGTCATGAATATGAAGGATAATCTTGGAAACTCAGTTCTTCATGTGGTGGAATGGCTAGTTGGCATTGGAACTGCTCCAGGGGGTGTGTTGGAAGTAGACAGTGTAAATCAGAGGGGTCTCACTGCTCTTGATCTGCTACTACTTTTCCCTAGTGAAGCCGGTGATAGGGAAATTGAGGAGATCCTTCGTGATGCTGGAGCTTTGAGAGCACGAGAGAGAGTCCAATCTAGTTCTGATGTACCTTCACATGCTCAAAACCATAGCCAAATGGCATCAGAGACTCATCAACAGCAGCCAAATAATGTGGCTTCGACAGCACTAGATACAGTCCATTCTAGTTCGGGTCAGAATCATAACCAAATGGCACAACAGGCCCATCAAGAGCAGGTGGCTCTGACAGCAATAGATATAGTCCATCCTAGTTCTGATAATCATAACCAAATGGCACCACAAGCCCATCAAGAGCAGGGTGCTTCGACAGCGATAGATATAGTCCATCCGAGTTCTGATGTGCCTTCACTTAATCAGAATCATAACCAAATAGCACCACAGACCACTCAAGAACAGCCAAATAATGTGGCTTCGACAACAGTAGCTCCAGTCCATCCTGTTTCTGCTCAAATCACTGGCTCTCCTAGTCAGAACCGCACTCAAATTGGATCACAGACTGATCAAGAGGAGCTAGACGATATGGAGTACTTCAAATTCAAAAAGGGTATAGACCCCGTTGATAATGCACGTACCGCGCTGTTAGTTATAGCTGTATTAGTGGCTACAGCGAGCTATGAAATTGCACTCAACCCTCCAGGCGGTCTTTGGTCTGACACAAACTTGAGCACAAATGGCACTGAGCCACCACACCTTGCAGGGACTTCCAATATAGCTTCTTATTTTCCAGTTTACACCATCTTTGGAGCCATTTTAAACTGCATTGGGTTTTCAGTTTCTCTTCACATGATCTATGTCCTCACAACCAATTTCCCCTTGCGGTTGGAGCTTATTGTTTGTGGCCTTGCAATGTACGTAACTTACATCAATGGATTGATTACTATTTCTCCAGGTGAGACCGCAGGTTGGTACTCACTGTTTGCACTTACGCCAGCTTTGCCTCCGGTGGTATTATTTGCTGCCAGAAGGATTAGGAAACTTTTGATCAAGCTTAGAGACCTTGCTGCCAGAACGGTTGGGAAACTTTGGATCAAGCTTAAAACCCTTCTAGTTAGCCAGTGGCCGAATTTGAATGTTGCACCATACATGTCAAGGAAGTCCTTGTCATACGCAACAGCTAATTTTAACCGAGATTATTCTCTGTTGCTTGCAGAGAATCCACTCCTACTCCATAGTCTTGCTCTAGATTCTGCAGAGAACCCGGTACATGTTGCTTCGCTTGCTGACCATGTTGGCTTTGTTAAGGAGATTCTAAGGTTGAAACCGGCTTTGGCCAAAGAAATGAACCCAGATGGCTTTAGCCCTATGCATATAGCATCTGCCAACGGATATTTGGAGATTGTAGGGGAGCTTCTGAAAGTTGATCTAAGACTGTGCCGGCTAAAAGGGAGAGATCAATGGACTCCTCTTCATCATGCTGCAAGGAGAGGAAGGGTAGATATTGTCAGAGAAATAGTCTTGGTTTGTCCAGAAAGTGTTGAAGATGTGACCATAAAAGGGGAAACTGCTTTGCACCTTGCTGTTAAGAACAGTCAGTTTGAAGCTGTTGAAGTTTTGGTGAAATCGGTCACAGAAATGAGGAAGGTGAACATCCTGAATTTGAAGGATAAGCATGGCAATACAGTTCTTCACCTAGCAACATGGAGAAAACAACACCAGTCTACCTGTCCTCGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

1285

Amino Acids

142.83

Weight (kDa)

8.78

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 101 - 148 1.2e-07 Tim17/Tim22/Tim23/Pmp24 family
TMEM135_C_rich PF15982 277 - 405 5.8e-08 N-terminal cysteine-rich region of Transmembrane protein 135
Ank_2 PF12796 508 - 592 2e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 601 - 662 1.2e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 610 - 660 1.1e-06 Ankyrin repeats (many copies)
PGG PF13962 914 - 997 3e-11 Domain of unknown function
Ank_2 PF12796 1129 - 1211 3.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 1196 - 1277 8.1e-11 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AarI CACCTGC 2 cut(s) 1239, 2386
Acc36I ACCTGC 4 cut(s) 413, 1239, 2386, 3071
AccB1I GGYRCC 1 cut(s) 2455
AccI GTMKAC 3 cut(s) 702, 2106, 3839
AccII CGCG 4 cut(s) 75, 96, 271, 2756
AccIII TCCGGA 1 cut(s) 1610
AclWI GGATC 4 cut(s) 1571, 2185, 2677, 3215
AcoI YGGCCR 3 cut(s) 1692, 3239, 3450
AcsI RAATTY 6 cut(s) 665, 847, 1655, 2714, 3244, 3775
AcuI CTGAAG 1 cut(s) 1491
AcyI GRCGYC 3 cut(s) 57, 99, 241
AfiI CCNNNNNNNGG 6 cut(s) 76, 1193, 2175, 2819, 3084, 3675
AflIII ACRYGT 2 cut(s) 3264, 3385
AjiI CACGTC 1 cut(s) 1906
AjnI CCWGG 6 cut(s) 960, 1510, 1797, 2086, 2815, 3067
AjuI GAANNNNNNNTTGG 8 cut(s) 1673, 1705, 1801, 1833, 2052, 2084, 3431, 3463
AloI GAACNNNNNNTCC 2 cut(s) 2028, 2060
Alw21I GWGCWC 5 cut(s) 114, 610, 864, 2221, 2849
Alw26I GTCTC 9 cut(s) 21, 286, 392, 1566, 1916, 2132, 2282, 3068, 3168
Alw44I GTGCAC 2 cut(s) 606, 860
AlwI GGATC 4 cut(s) 1571, 2185, 2677, 3215
AlwNI CAGNNNCTG 1 cut(s) 1588
Aor13HI TCCGGA 1 cut(s) 1610
AoxI GGCC 8 cut(s) 78, 708, 966, 1692, 2382, 3019, 3239, 3450
ApaLI GTGCAC 2 cut(s) 606, 860
ApoI RAATTY 6 cut(s) 665, 847, 1655, 2714, 3244, 3775
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 4 cut(s) 120, 129, 273, 2758
AspS9I GGNCC 4 cut(s) 85, 683, 967, 2383
AsuC2I CCSGG 1 cut(s) 3380
AsuHPI GGTGA 8 cut(s) 1175, 1220, 1678, 2183, 3083, 3748, 3775, 3800
AsuNHI GCTAGC 2 cut(s) 1361, 1603
AvaII GGWCC 2 cut(s) 85, 683
AxyI CCTNAGG 1 cut(s) 1334
BaeGI GKGCMC 2 cut(s) 610, 864
BaeI ACNNNNGTAYC 2 cut(s) 912, 945
BalI TGGCCA 2 cut(s) 1694, 3452
BanI GGYRCC 1 cut(s) 2455
BanII GRGCYC 2 cut(s) 114, 1771
BarI GAAGNNNNNNTAC 2 cut(s) 3513, 3545
BauI CACGAG 1 cut(s) 2220
BbsI GAAGAC 2 cut(s) 683, 1908
Bbv12I GWGCWC 5 cut(s) 114, 610, 864, 2221, 2849
BbvCI CCTCAGC 1 cut(s) 1254
BceAI ACGGC 1 cut(s) 695
BciT130I CCWGG 6 cut(s) 962, 1512, 1799, 2088, 2817, 3069
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 2 cut(s) 2680, 3162
BcnI CCSGG 1 cut(s) 3380
BcoDI GTCTC 9 cut(s) 21, 286, 392, 1566, 1916, 2132, 2282, 3068, 3168
BfmI CTRYAG 4 cut(s) 950, 1772, 2784, 3369
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 4 cut(s) 413, 1239, 2386, 3071
BfuI GTATCC 1 cut(s) 725
BglI GCCNNNNNGGC 2 cut(s) 324, 755
BlpI GCTNAGC 1 cut(s) 693
BmcAI AGTACT 1 cut(s) 2708
Bme1390I CCNGG 7 cut(s) 962, 1512, 1799, 2088, 2817, 3069, 3380
Bme18I GGWCC 2 cut(s) 85, 683
BmgBI CACGTC 1 cut(s) 1906
BmgT120I GGNCC 4 cut(s) 85, 683, 967, 2383
BmiI GGNNCC 5 cut(s) 210, 969, 1326, 2457, 2926
BmrFI CCNGG 7 cut(s) 962, 1512, 1799, 2088, 2817, 3069, 3380
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 9 cut(s) 359, 1088, 1420, 1567, 1745, 1840, 2191, 2291, 3503
BmtI GCTAGC 2 cut(s) 1365, 1607
BmuI ACTGGG 1 cut(s) 731
BpiI GAAGAC 2 cut(s) 683, 1908
BpmI CTGGAG 9 cut(s) 98, 589, 1163, 1775, 2070, 2226, 2592, 2799, 3051
Bpu10I CCTNAGC 1 cut(s) 1254
Bpu1102I GCTNAGC 1 cut(s) 693
BpuEI CTTGAG 3 cut(s) 1544, 2553, 2863
BpuMI CCSGG 1 cut(s) 3380
BsaAI YACGTR 2 cut(s) 2750, 3034
BsaHI GRCGYC 3 cut(s) 57, 99, 241
BsaI GGTCTC 3 cut(s) 2132, 3068, 3168
BsaJI CCNNGG 6 cut(s) 81, 1187, 1511, 1725, 1797, 2087
BsaWI WCCGGW 3 cut(s) 1378, 1610, 3121
Bsc4I CCNNNNNNNGG 6 cut(s) 76, 1193, 2175, 2819, 3084, 3675
Bse118I RCCGGY 3 cut(s) 2168, 3442, 3555
Bse21I CCTNAGG 1 cut(s) 1334
Bse3DI GCAATG 1 cut(s) 3033
BseAI TCCGGA 1 cut(s) 1610
BseBI CCWGG 6 cut(s) 962, 1512, 1799, 2088, 2817, 3069
BseDI CCNNGG 6 cut(s) 81, 1187, 1511, 1725, 1797, 2087
BseGI GGATG 8 cut(s) 809, 835, 1717, 1735, 2425, 2506, 2614, 3768
BseLI CCNNNNNNNGG 6 cut(s) 76, 1193, 2175, 2819, 3084, 3675
BseMI GCAATG 1 cut(s) 3033
BseMII CTCAG 8 cut(s) 630, 707, 762, 1245, 1325, 1473, 2055, 2850
BseRI GAGGAG 5 cut(s) 122, 219, 2201, 2702, 3573
BseSI GKGCMC 2 cut(s) 610, 864
BseYI CCCAGC 1 cut(s) 150
Bsh1236I CGCG 4 cut(s) 75, 96, 271, 2756
BshFI GGCC 8 cut(s) 80, 710, 968, 1694, 2384, 3021, 3241, 3452
BshNI GGYRCC 1 cut(s) 2455
BsiHKAI GWGCWC 5 cut(s) 114, 610, 864, 2221, 2849
BsiSI CCGG 7 cut(s) 1379, 1611, 2169, 3122, 3380, 3443, 3556
BslFI GGGAC 1 cut(s) 2893
BslI CCNNNNNNNGG 6 cut(s) 76, 1193, 2175, 2819, 3084, 3675
BsmAI GTCTC 9 cut(s) 21, 286, 392, 1566, 1916, 2132, 2282, 3068, 3168
BsmBI CGTCTC 2 cut(s) 21, 1566
BsmFI GGGAC 1 cut(s) 2893
BsmI GAATGC 4 cut(s) 415, 1019, 1255, 1405
BsnI GGCC 8 cut(s) 80, 710, 968, 1694, 2384, 3021, 3241, 3452
Bso31I GGTCTC 3 cut(s) 2132, 3068, 3168
Bsp1286I GDGCHC 6 cut(s) 114, 610, 864, 1771, 2221, 2849
Bsp13I TCCGGA 1 cut(s) 1610
Bsp1720I GCTNAGC 1 cut(s) 693
Bsp19I CCATGG 1 cut(s) 1725
BspANI GGCC 8 cut(s) 80, 710, 968, 1694, 2384, 3021, 3241, 3452
BspCNI CTCAG 8 cut(s) 631, 706, 763, 1246, 1326, 1474, 2054, 2851
BspEI TCCGGA 1 cut(s) 1610
BspFNI CGCG 4 cut(s) 75, 96, 271, 2756
BspHI TCATGA 2 cut(s) 661, 2013
BspLI GGNNCC 5 cut(s) 210, 969, 1326, 2457, 2926
BspMAI CTGCAG 2 cut(s) 1776, 3373
BspMI ACCTGC 4 cut(s) 413, 1239, 2386, 3071
BspOI GCTAGC 2 cut(s) 1365, 1607
BspPI GGATC 4 cut(s) 1571, 2185, 2677, 3215
BspT107I GGYRCC 1 cut(s) 2455
BspTNI GGTCTC 3 cut(s) 2132, 3068, 3168
BsrDI GCAATG 1 cut(s) 3033
BsrFI RCCGGY 3 cut(s) 2168, 3442, 3555
BssAI RCCGGY 3 cut(s) 2168, 3442, 3555
BssECI CCNNGG 6 cut(s) 81, 1187, 1511, 1725, 1797, 2087
BssNAI GTATAC 1 cut(s) 703
BssNI GRCGYC 3 cut(s) 57, 99, 241
BssSI CACGAG 1 cut(s) 2220
BssT1I CCWWGG 1 cut(s) 1725
Bst1107I GTATAC 1 cut(s) 703
Bst2BI CACGAG 1 cut(s) 2220
Bst2UI CCWGG 6 cut(s) 962, 1512, 1799, 2088, 2817, 3069
Bst6I CTCTTC 4 cut(s) 795, 1840, 2964, 3591
BstACI GRCGYC 3 cut(s) 57, 99, 241
BstAPI GCANNNNNTGC 2 cut(s) 515, 1564
BstBAI YACGTR 2 cut(s) 2750, 3034
BstDSI CCRYGG 2 cut(s) 1187, 1725
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 8 cut(s) 809, 835, 1717, 1735, 2425, 2506, 2614, 3768
BstFNI CGCG 4 cut(s) 75, 96, 271, 2756
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 4 cut(s) 120, 129, 273, 2758
BstMAI GTCTC 9 cut(s) 21, 286, 392, 1566, 1916, 2132, 2282, 3068, 3168
BstNI CCWGG 6 cut(s) 962, 1512, 1799, 2088, 2817, 3069
BstNSI RCATGY 6 cut(s) 318, 606, 2012, 2261, 3268, 3389
BstSCI CCNGG 7 cut(s) 960, 1510, 1797, 2086, 2815, 3067, 3378
BstSFI CTRYAG 4 cut(s) 950, 1772, 2784, 3369
BstSLI GKGCMC 2 cut(s) 610, 864
BstSNI TACGTA 1 cut(s) 3034
BstUI CGCG 4 cut(s) 75, 96, 271, 2756
BstV2I GAAGAC 2 cut(s) 683, 1908
BstX2I RGATCY 1 cut(s) 2190
BstXI CCANNNNNNTGG 3 cut(s) 204, 680, 1732
BstYI RGATCY 1 cut(s) 2190
BstZ17I GTATAC 1 cut(s) 703
Bsu36I CCTNAGG 1 cut(s) 1334
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 8 cut(s) 80, 710, 968, 1694, 2384, 3021, 3241, 3452
BtgI CCRYGG 2 cut(s) 1187, 1725
BtrI CACGTC 1 cut(s) 1906
BtsCI GGATG 8 cut(s) 809, 835, 1717, 1735, 2425, 2506, 2614, 3768
BtsI GCAGTG 2 cut(s) 1826, 2130
BveI ACCTGC 4 cut(s) 413, 1239, 2386, 3071
CaiI CAGNNNCTG 1 cut(s) 1588
CciI TCATGA 2 cut(s) 661, 2013
CfoI GCGC 4 cut(s) 120, 129, 273, 2758
Cfr10I RCCGGY 3 cut(s) 2168, 3442, 3555
Cfr13I GGNCC 4 cut(s) 85, 683, 967, 2383
CseI GACGC 5 cut(s) 65, 107, 179, 230, 623
CspCI CAANNNNNGTGG 4 cut(s) 2295, 2330, 2568, 2603
DraI TTTAAA 1 cut(s) 2934
EaeI YGGCCR 3 cut(s) 1692, 3239, 3450
Eam1104I CTCTTC 4 cut(s) 795, 1840, 2964, 3591
EarI CTCTTC 4 cut(s) 795, 1840, 2964, 3591
EciI GGCGGA 1 cut(s) 490
Ecl136II GAGCTC 1 cut(s) 112
Eco105I TACGTA 1 cut(s) 3034
Eco130I CCWWGG 1 cut(s) 1725
Eco24I GRGCYC 2 cut(s) 114, 1771
Eco31I GGTCTC 3 cut(s) 2132, 3068, 3168
Eco47I GGWCC 2 cut(s) 85, 683
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 1491
Eco81I CCTNAGG 1 cut(s) 1334
EcoICRI GAGCTC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoRII CCWGG 6 cut(s) 960, 1510, 1797, 2086, 2815, 3067
EcoT14I CCWWGG 1 cut(s) 1725
EcoT22I ATGCAT 1 cut(s) 3489
EcoT38I GRGCYC 2 cut(s) 114, 1771
ErhI CCWWGG 1 cut(s) 1725
Esp3I CGTCTC 2 cut(s) 21, 1566
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 1700, 1732
FaqI GGGAC 1 cut(s) 2893
FauI CCCGC 3 cut(s) 48, 149, 427
FbaI TGATCA 2 cut(s) 2680, 3162
FblI GTMKAC 3 cut(s) 702, 2106, 3839
FokI GGATG 8 cut(s) 796, 822, 1724, 1742, 2412, 2493, 2601, 3755
FriOI GRGCYC 2 cut(s) 114, 1771
GlaI GCGC 4 cut(s) 119, 128, 272, 2757
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 9 cut(s) 98, 589, 1163, 1775, 2070, 2226, 2592, 2799, 3051
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 8 cut(s) 80, 710, 968, 1694, 2384, 3021, 3241, 3452
HapII CCGG 7 cut(s) 1379, 1611, 2169, 3122, 3380, 3443, 3556
HgaI GACGC 5 cut(s) 65, 107, 179, 230, 623
HhaI GCGC 4 cut(s) 120, 129, 273, 2758
Hin1I GRCGYC 3 cut(s) 57, 99, 241
Hin6I GCGC 4 cut(s) 118, 127, 271, 2756
HinP1I GCGC 4 cut(s) 118, 127, 271, 2756
HindIII AAGCTT 4 cut(s) 1094, 1779, 3167, 3212
HpaII CCGG 7 cut(s) 1379, 1611, 2169, 3122, 3380, 3443, 3556
HphI GGTGA 8 cut(s) 1175, 1220, 1678, 2183, 3083, 3748, 3775, 3800
Hpy166II GTNNAC 9 cut(s) 608, 703, 862, 1166, 1222, 2107, 2911, 3766, 3840
Hpy8I GTNNAC 9 cut(s) 608, 703, 862, 1166, 1222, 2107, 2911, 3766, 3840
Hpy99I CGWCG 6 cut(s) 10, 16, 101, 183, 195, 1040
HpyCH4IV ACGT 4 cut(s) 923, 1905, 2749, 3033
HpySE526I ACGT 4 cut(s) 923, 1905, 2749, 3033
Hsp92I GRCGYC 3 cut(s) 57, 99, 241
HspAI GCGC 4 cut(s) 118, 127, 271, 2756
Kpn2I TCCGGA 1 cut(s) 1610
KroI GCCGGC 1 cut(s) 3555
KroNI GCCGGC 1 cut(s) 3557
Ksp22I TGATCA 2 cut(s) 2680, 3162
LweI GCATC 9 cut(s) 359, 1088, 1420, 1567, 1745, 1840, 2191, 2291, 3503
MaeII ACGT 4 cut(s) 923, 1905, 2749, 3033
MaeIII GTNAC 6 cut(s) 1226, 1906, 1929, 3034, 3664, 3745
MfeI CAATTG 2 cut(s) 429, 1562
MflI RGATCY 1 cut(s) 2190
MhlI GDGCHC 6 cut(s) 114, 610, 864, 1771, 2221, 2849
MlsI TGGCCA 2 cut(s) 1694, 3452
MluNI TGGCCA 2 cut(s) 1694, 3452
MlyI GAGTC 7 cut(s) 192, 455, 1816, 1980, 2238, 2284, 3574
MmeI TCCRAC 6 cut(s) 87, 777, 1018, 1959, 2079, 2982
Mox20I TGGCCA 2 cut(s) 1694, 3452
Mph1103I ATGCAT 1 cut(s) 3489
MroI TCCGGA 1 cut(s) 1610
MroNI GCCGGC 1 cut(s) 3555
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 2 cut(s) 1694, 3452
MseI TTAA 9 cut(s) 996, 1245, 1944, 2532, 2933, 3216, 3303, 3420, 3702
MslI CAYNNNNRTG 4 cut(s) 983, 1730, 2018, 3752
Msp20I TGGCCA 2 cut(s) 1694, 3452
MspA1I CMGCKG 1 cut(s) 1640
MspI CCGG 7 cut(s) 1379, 1611, 2169, 3122, 3380, 3443, 3556
MspR9I CCNGG 7 cut(s) 962, 1512, 1799, 2088, 2817, 3069, 3380
MunI CAATTG 2 cut(s) 429, 1562
Mva1269I GAATGC 4 cut(s) 415, 1019, 1255, 1405
MvaI CCWGG 6 cut(s) 962, 1512, 1799, 2088, 2817, 3069
MvnI CGCG 4 cut(s) 75, 96, 271, 2756
NaeI GCCGGC 1 cut(s) 3557
NciI CCSGG 1 cut(s) 3380
NcoI CCATGG 1 cut(s) 1725
NgoMIV GCCGGC 1 cut(s) 3555
NheI GCTAGC 2 cut(s) 1361, 1603
NlaIV GGNNCC 5 cut(s) 210, 969, 1326, 2457, 2926
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 4 cut(s) 1226, 1906, 3664, 3745
NsiI ATGCAT 1 cut(s) 3489
NspI RCATGY 6 cut(s) 318, 606, 2012, 2261, 3268, 3389
PaeI GCATGC 1 cut(s) 318
PagI TCATGA 2 cut(s) 661, 2013
PaqCI CACCTGC 2 cut(s) 1239, 2386
PciI ACATGT 2 cut(s) 3264, 3385
PcsI WCGNNNNNNNCGW 2 cut(s) 178, 187
PctI GAATGC 4 cut(s) 415, 1019, 1255, 1405
PdiI GCCGGC 1 cut(s) 3557
PdmI GAANNNNTTC 1 cut(s) 804
PleI GAGTC 7 cut(s) 191, 455, 1816, 1979, 2237, 2284, 3574
PpsI GAGTC 7 cut(s) 191, 455, 1816, 1979, 2237, 2284, 3574
Ppu21I YACGTR 2 cut(s) 2750, 3034
PscI ACATGT 2 cut(s) 3264, 3385
PsiI TTATAA 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 6 cut(s) 960, 1510, 1797, 2086, 2815, 3067
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 6 cut(s) 960, 1510, 1797, 2086, 2815, 3067
PspN4I GGNNCC 5 cut(s) 210, 969, 1326, 2457, 2926
PspPI GGNCC 4 cut(s) 85, 683, 967, 2383
PstI CTGCAG 2 cut(s) 1776, 3373
PstNI CAGNNNCTG 1 cut(s) 1588
PsuI RGATCY 1 cut(s) 2190
RseI CAYNNNNRTG 4 cut(s) 983, 1730, 2018, 3752
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 9 cut(s) 996, 1245, 1944, 2532, 2933, 3216, 3303, 3420, 3702
Sau96I GGNCC 4 cut(s) 85, 683, 967, 2383
ScaI AGTACT 1 cut(s) 2708
SchI GAGTC 7 cut(s) 192, 455, 1816, 1980, 2238, 2284, 3574
ScrFI CCNGG 7 cut(s) 962, 1512, 1799, 2088, 2817, 3069, 3380
SduI GDGCHC 6 cut(s) 114, 610, 864, 1771, 2221, 2849
SfaNI GCATC 9 cut(s) 359, 1088, 1420, 1567, 1745, 1840, 2191, 2291, 3503
SfcI CTRYAG 4 cut(s) 950, 1772, 2784, 3369
SinI GGWCC 2 cut(s) 85, 683
SmiMI CAYNNNNRTG 4 cut(s) 983, 1730, 2018, 3752
SmlI CTYRAG 3 cut(s) 1523, 2568, 2842
SmoI CTYRAG 3 cut(s) 1523, 2568, 2842
SnaBI TACGTA 1 cut(s) 3034
SphI GCATGC 1 cut(s) 318
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 7 cut(s) 960, 1510, 1797, 2086, 2815, 3067, 3378
StyI CCWWGG 1 cut(s) 1725
TaiI ACGT 4 cut(s) 926, 1908, 2752, 3036
TaqI TCGA 5 cut(s) 1082, 1662, 2321, 2486, 2594
TaqII GACCGA 1 cut(s) 3733
TatI WGTACW 4 cut(s) 384, 1293, 1491, 2706
TauI GCSGC 7 cut(s) 46, 49, 123, 137, 140, 968, 1353
Tru1I TTAA 9 cut(s) 996, 1245, 1944, 2532, 2933, 3216, 3303, 3420, 3702
Tru9I TTAA 9 cut(s) 996, 1245, 1944, 2532, 2933, 3216, 3303, 3420, 3702
TseFI GTSAC 4 cut(s) 1226, 1906, 3664, 3745
Tsp45I GTSAC 4 cut(s) 1226, 1906, 3664, 3745
TspDTI ATGAA 9 cut(s) 678, 824, 1451, 2030, 2036, 2039, 2811, 3476, 3578
TspGWI ACGGA 1 cut(s) 3519
VneI GTGCAC 2 cut(s) 606, 860
VpaK11BI GGWCC 2 cut(s) 85, 683
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 6 cut(s) 665, 847, 1655, 2714, 3244, 3775
XbaI TCTAGA 2 cut(s) 1672, 3361
XceI RCATGY 6 cut(s) 318, 606, 2012, 2261, 3268, 3389
XcmI CCANNNNNNNNNTGG 4 cut(s) 572, 1750, 2823, 3508
XmiI GTMKAC 3 cut(s) 702, 2106, 3839
XmnI GAANNNNTTC 1 cut(s) 804
ZrmI AGTACT 1 cut(s) 2708
Zsp2I ATGCAT 1 cut(s) 3489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.