Rorug01G0376800

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000281
Physical Location & Seq
Forward (+)
49045964 .. 49050772
4809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0376800.1

Sequence Viewer

Length: 1494 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACGGATCGAGAAACACGATGCCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAAAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTCTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCAAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCTATGAGACAGAGAGGTGGATGGAACAGGTATCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAAGGCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTCGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGCAGAAAGACGTATTGAAAAAAGTTGTGTTGAGAACATGAGAAGATTTAAACTAAACAAATTGAGAGCTCGGAATAAAAGTTCTTTGTTTGAGCTTTTTATTTCATTCCTTGGGAAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATTGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTATATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATACTGCAAGAGGTGTCAGCCAAAAAGCATTGAAAAGGATCTCTGAAGTTTTTGAGATGAGTCGTGACAGGCTAATCGCAGCTCGTTACAATGCCTCCTTAGCCATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATACGCTACATCTTATTAGCAACCCAGGTTACAATATTAAGCACCACCAAACAACTCATCCACAATATCTCAGGGCTGAGAGCTCACATTCACGAACCCCAAGACATTATAACGGTCCCCGGAAACCAAATGGTAAGGTACGAGAGTTGGTGCCCCCAAGGGATTATAATGGCCCCCCAAAACCAAGTGGTCCTTCTGGACGGGAGTTGGTAGACTCATCCCCACAAGTACATCAACAACTACGAAAGATGGTGCACTCATCCTCACAAGTACAGCCACAAATACAGAAGCTGGTGCAGTCACCGTCACAAGTACAACCGCATATACGAAAGTCTGTGCCCTCAACCTCACGAGTACAATCACCACTTCAGAAAGTGAAGACAGAGAAGGCACCAATGCTACAGGCGTATCAGGAATGGAGACCAAAGAAACCAGGAATGCAGACCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

497

Amino Acids

56.83

Weight (kDa)

9.81

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 3.5e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 1209
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 2 cut(s) 1192, 1432
AccI GTMKAC 1 cut(s) 1254
AciI CCGC 2 cut(s) 788, 1361
AclWI GGATC 5 cut(s) 60, 151, 431, 875, 947
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 2 cut(s) 72, 798
AcuI CTGAAG 2 cut(s) 966, 1394
AfaI GTAC 7 cut(s) 252, 568, 1182, 1272, 1314, 1356, 1398
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 9 cut(s) 38, 192, 504, 520, 593, 662, 833, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 1066, 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 1219, 1251
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 7 cut(s) 95, 114, 713, 739, 983, 1125, 1333
AluI AGCT 7 cut(s) 95, 114, 713, 739, 983, 1125, 1333
Alw21I GWGCWC 3 cut(s) 715, 1127, 1299
Alw26I GTCTC 4 cut(s) 157, 171, 271, 1456
Alw44I GTGCAC 1 cut(s) 1295
AlwI GGATC 5 cut(s) 60, 151, 431, 875, 947
AlwNI CAGNNNCTG 1 cut(s) 1333
AoxI GGCC 3 cut(s) 394, 893, 1213
ApaLI GTGCAC 1 cut(s) 1295
ApeKI GCWGC 2 cut(s) 785, 980
ApoI RAATTY 2 cut(s) 72, 798
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 1157, 1214, 1232
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 3 cut(s) 478, 1335, 1395
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 2 cut(s) 1157, 1232
BaeGI GKGCMC 3 cut(s) 1197, 1299, 1383
BaeI ACNNNNGTAYC 4 cut(s) 1172, 1205, 1433, 1466
BanI GGYRCC 2 cut(s) 1192, 1432
BanII GRGCYC 2 cut(s) 715, 1127
BauI CACGAG 2 cut(s) 542, 1392
BbsI GAAGAC 1 cut(s) 1427
Bbv12I GWGCWC 3 cut(s) 715, 1127, 1299
BbvI GCAGC 2 cut(s) 772, 992
BccI CCATC 4 cut(s) 285, 331, 840, 1285
BciT130I CCWGG 2 cut(s) 1068, 1476
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 4 cut(s) 157, 171, 271, 1456
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 2 cut(s) 105, 1442
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 2 cut(s) 786, 981
BlsI GCNGC 2 cut(s) 787, 982
Bme1390I CCNGG 3 cut(s) 1068, 1162, 1476
Bme18I GGWCC 2 cut(s) 1157, 1232
BmgT120I GGNCC 3 cut(s) 1157, 1214, 1232
BmiI GGNNCC 5 cut(s) 135, 1159, 1194, 1216, 1434
BmrFI CCNGG 3 cut(s) 1068, 1162, 1476
BmsI GCATC 2 cut(s) 56, 634
BpiI GAAGAC 1 cut(s) 1427
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BpuMI CCSGG 1 cut(s) 1162
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 1456
BsaJI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1199
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 2 cut(s) 1068, 1476
BseDI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1199
BseGI GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1259, 1301
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 5 cut(s) 28, 168, 524, 1110, 1126
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 3 cut(s) 1197, 1299, 1383
BseXI GCAGC 2 cut(s) 772, 992
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 1358
BshFI GGCC 3 cut(s) 396, 895, 1215
BshNI GGYRCC 2 cut(s) 1192, 1432
BsiHKAI GWGCWC 3 cut(s) 715, 1127, 1299
BsiSI CCGG 1 cut(s) 1162
BslFI GGGAC 2 cut(s) 526, 1143
BsmAI GTCTC 4 cut(s) 157, 171, 271, 1456
BsmFI GGGAC 2 cut(s) 526, 1143
BsmI GAATGC 1 cut(s) 1485
BsnI GGCC 3 cut(s) 396, 895, 1215
Bso31I GGTCTC 1 cut(s) 1456
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 5 cut(s) 715, 1127, 1197, 1299, 1383
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BspACI CCGC 2 cut(s) 788, 1361
BspANI GGCC 3 cut(s) 396, 895, 1215
BspCNI CTCAG 5 cut(s) 27, 167, 525, 1111, 1125
BspLI GGNNCC 5 cut(s) 135, 1159, 1194, 1216, 1434
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 60, 151, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 2 cut(s) 1192, 1432
BspTI CTTAAG 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 1456
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1199
BssMI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BssSI CACGAG 2 cut(s) 542, 1392
BssT1I CCWWGG 3 cut(s) 754, 772, 1199
Bst2BI CACGAG 2 cut(s) 542, 1392
Bst2UI CCWGG 2 cut(s) 1068, 1476
Bst4CI ACNGT 3 cut(s) 130, 1157, 1347
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstDEI CTNAG 6 cut(s) 14, 154, 533, 1000, 1112, 1119
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1259, 1301
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 8 cut(s) 55, 146, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 4 cut(s) 157, 171, 271, 1456
BstMBI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 2 cut(s) 1068, 1476
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 3 cut(s) 1066, 1160, 1474
BstSFI CTRYAG 2 cut(s) 105, 1442
BstSLI GKGCMC 3 cut(s) 1197, 1299, 1383
BstV1I GCAGC 2 cut(s) 772, 992
BstV2I GAAGAC 1 cut(s) 1427
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 3 cut(s) 396, 895, 1215
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1259, 1301
BveI ACCTGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 1333
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 1157, 1214, 1232
Csp6I GTAC 7 cut(s) 251, 567, 1181, 1271, 1313, 1355, 1397
CviAII CATG 2 cut(s) 564, 682
CviQI GTAC 7 cut(s) 251, 567, 1181, 1271, 1313, 1355, 1397
DdeI CTNAG 6 cut(s) 14, 154, 533, 1000, 1112, 1119
DpnI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
DpnII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 2 cut(s) 713, 1125
Eco130I CCWWGG 3 cut(s) 754, 772, 1199
Eco24I GRGCYC 2 cut(s) 715, 1127
Eco31I GGTCTC 1 cut(s) 1456
Eco47I GGWCC 2 cut(s) 1157, 1232
Eco53kI GAGCTC 2 cut(s) 713, 1125
Eco57I CTGAAG 2 cut(s) 966, 1394
EcoICRI GAGCTC 2 cut(s) 713, 1125
EcoRII CCWGG 2 cut(s) 1066, 1474
EcoT14I CCWWGG 3 cut(s) 754, 772, 1199
EcoT38I GRGCYC 2 cut(s) 715, 1127
ErhI CCWWGG 3 cut(s) 754, 772, 1199
FaeI CATG 2 cut(s) 567, 685
FalI AAGNNNNNCTT 2 cut(s) 1219, 1251
FaqI GGGAC 2 cut(s) 526, 1143
FatI CATG 2 cut(s) 563, 681
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 2 cut(s) 786, 981
FokI GGATG 9 cut(s) 197, 303, 436, 632, 653, 858, 1086, 1246, 1288
FriOI GRGCYC 2 cut(s) 715, 1127
Fsp4HI GCNGC 2 cut(s) 786, 981
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 786, 981
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 3 cut(s) 396, 895, 1215
HapII CCGG 1 cut(s) 1162
HhaI GCGC 1 cut(s) 212
Hin1II CATG 2 cut(s) 567, 685
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 3 cut(s) 101, 961, 1256
HpaII CCGG 1 cut(s) 1162
HphI GGTGA 3 cut(s) 478, 1335, 1395
Hpy166II GTNNAC 3 cut(s) 569, 1255, 1297
Hpy8I GTNNAC 3 cut(s) 569, 1255, 1297
HpyAV CCTTC 6 cut(s) 262, 463, 775, 1028, 1245, 1423
HpyCH4III ACNGT 3 cut(s) 130, 1157, 1347
HpyCH4IV ACGT 2 cut(s) 28, 655
HpyCH4V TGCA 9 cut(s) 125, 320, 575, 647, 804, 908, 1297, 1339, 1483
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 6 cut(s) 14, 154, 533, 1000, 1112, 1119
HpySE526I ACGT 2 cut(s) 28, 655
Hsp92II CATG 2 cut(s) 567, 685
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
LmnI GCTCC 2 cut(s) 92, 133
Lsp1109I GCAGC 2 cut(s) 772, 992
LweI GCATC 2 cut(s) 56, 634
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 2 cut(s) 28, 655
MaeIII GTNAC 6 cut(s) 84, 965, 986, 1070, 1341, 1347
MalI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
MboI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
MboII GAAGA 8 cut(s) 68, 213, 243, 541, 581, 699, 890, 1432
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 5 cut(s) 715, 1127, 1197, 1299, 1383
MluCI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 828
MlyI GAGTC 3 cut(s) 110, 970, 1250
MmeI TCCRAC 1 cut(s) 1060
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1080
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspI CCGG 1 cut(s) 1162
MspR9I CCNGG 3 cut(s) 1068, 1162, 1476
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 1485
MvaI CCWGG 2 cut(s) 1068, 1476
MwoI GCNNNNNNNGC 1 cut(s) 1001
NciI CCSGG 1 cut(s) 1162
NdeII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
NlaIII CATG 2 cut(s) 567, 685
NlaIV GGNNCC 5 cut(s) 135, 1159, 1194, 1216, 1434
NmuCI GTSAC 3 cut(s) 965, 1341, 1347
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 779
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 1485
PkrI GCNGC 2 cut(s) 787, 982
PleI GAGTC 3 cut(s) 109, 969, 1250
PpsI GAGTC 3 cut(s) 109, 969, 1250
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PsiI TTATAA 2 cut(s) 1152, 1209
Psp124BI GAGCTC 2 cut(s) 715, 1127
Psp6I CCWGG 2 cut(s) 1066, 1474
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 1066, 1474
PspN4I GGNNCC 5 cut(s) 135, 1159, 1194, 1216, 1434
PspPI GGNCC 3 cut(s) 1157, 1214, 1232
PstNI CAGNNNCTG 1 cut(s) 1333
PsuI RGATCY 3 cut(s) 436, 880, 939
RsaI GTAC 7 cut(s) 252, 568, 1182, 1272, 1314, 1356, 1398
RsaNI GTAC 7 cut(s) 251, 567, 1181, 1271, 1313, 1355, 1397
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 715, 1127
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1080
SatI GCNGC 2 cut(s) 786, 981
Sau3AI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 3 cut(s) 1157, 1214, 1232
SchI GAGTC 3 cut(s) 110, 970, 1250
ScrFI CCNGG 3 cut(s) 1068, 1162, 1476
SduI GDGCHC 5 cut(s) 715, 1127, 1197, 1299, 1383
SfaNI GCATC 2 cut(s) 56, 634
SfcI CTRYAG 2 cut(s) 105, 1442
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 2 cut(s) 1157, 1232
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 828
SsiI CCGC 2 cut(s) 788, 1361
SspI AATATT 1 cut(s) 1078
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 2 cut(s) 715, 1127
StyD4I CCNGG 3 cut(s) 1066, 1160, 1474
StyI CCWWGG 3 cut(s) 754, 772, 1199
TaaI ACNGT 3 cut(s) 130, 1157, 1347
TaiI ACGT 2 cut(s) 31, 658
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 828
TatI WGTACW 6 cut(s) 250, 566, 1270, 1312, 1354, 1396
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1080
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1080
TseFI GTSAC 3 cut(s) 965, 1341, 1347
TseI GCWGC 2 cut(s) 785, 980
Tsp45I GTSAC 3 cut(s) 965, 1341, 1347
TspDTI ATGAA 6 cut(s) 281, 298, 416, 440, 513, 738
TspGWI ACGGA 2 cut(s) 65, 632
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 1 cut(s) 1295
VpaK11BI GGWCC 2 cut(s) 1157, 1232
XapI RAATTY 2 cut(s) 72, 798
XceI RCATGY 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 1254
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.