MD15G1430200.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000281
Physical Location & Seq
Forward (+)
53078376 .. 53079920
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1430200.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGCGAGAGTCATGAGACTTGTTTCCCTACTCACCTTTCTTCTATCCATTGCGTCTTTTACACTTGCCTTATGCAATCGAAACCTTAATTCACCACCTTGCAAAGAAAATGAGAGACGAGCACTTCTTATGTTCAAGAAAGATCTCAATGATTCTTCAAATTTGCTTTCCTCTTGGGCTGGTGAAGGCGATTGTTGCAATTGGACCGGTGTTGCCTGCGACAATTTAACCGGTCATGTCCGTGAGCTCCACCTTGCTGGTAATTATGATGAAGAGATTGGTGAGTTTCATAGGCTGGGTGGGTTCAGCGGAACCATTCCTCATCAGTTGGGAAATCTCTCGAGCCTACGATATCTCAACCTTTTTGAAAACTATGGTTTGATGGTCGAGAATCTCGAATGGCTATCTGGTCTTTCTTTGTTGAGACATCTTGGCATGGGCTATGTAGATCTTACCAACGCATCTCATTGGTTACAAGTAAACACATTCCCTTCTCTGCTGGTCGATTTGCATTTGCCAAGTTGCGAACTTCATCACAAGCCAAGTGGTATTGCGAACTTGACAAGTCTTAAAGTTCTTGATCTATCCGAGAACTATTTCAACTCTATCATACCTACATGGTTGTACAGTTTGGGCCATCTTGAGTCCCTCGATCTTTCTTACAATGCTTTTCATGGTGAAATTTCGAGTTCCCTTGGAAACTTGACAGCCCTTGTTCATCTTAACTTAGCTGGTAATCAACTTAAAGGGGAAATCCCAAACTCATTGGGAAATCTTTGTAAGTTGACTTCTGTTGTTCTATGGTCAAATAATTTTACGGGGAGGGTATCAGAAATCTTTGAAAGTTTGTCTCAGTGTAGTTTTGATCAAATAGCTTATTTAGTTTTGGGATTTAATAGTTTTTCAGGGCATTTATCTGGTAAGCTAGGAATTTTTAAAAATTTAAGTTCTCTTGATCTTTCGTATAATTCTATATCAGGTCTCATTCCAGTGTTCTTAGGAAATCTATCACGCTTGCAATACTGTACCATTGATCACAATTCATTCGAGGGTGTTGTCTCTGAAGCTCATTTTAGTAATCTTACAAGATTGGATACCTTTACTGCAAATGAGAACTCCTTGACTCTTAAAACCAACTATGACTGGGTTCCTCCTTTTCAACTTTCTGAGTTGAGTTTAGGTTCTTGGCGTCTGGACCCATCAGAATTGCCTACATGGCTTCAAAGTCAAAATCAGTTGTCTGTTCTTAACATGTCCACTACAGGAATTTCAGGTACCATTCCGACTTGGTTCTGGAACATCTTTTTGCGCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

439

Amino Acids

48.78

Weight (kDa)

5.47

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 74 2.9e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 156 - 270 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 167 - 223 7.4e-09 Leucine rich repeat
LRR_8 PF13855 189 - 248 3.5e-10 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1274
AccB1I GGYRCC 1 cut(s) 1274
AccII CGCG 1 cut(s) 1311
AciI CCGC 1 cut(s) 309
AcsI RAATTY 5 cut(s) 160, 681, 930, 940, 1266
AcuI CTGAAG 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 1189
AfaI GTAC 3 cut(s) 626, 1027, 1276
AflIII ACRYGT 1 cut(s) 1251
AgeI ACCGGT 2 cut(s) 206, 230
AgsI TTSAA 7 cut(s) 136, 159, 368, 601, 842, 1160, 1223
AluBI AGCT 5 cut(s) 247, 731, 875, 925, 1067
AluI AGCT 5 cut(s) 247, 731, 875, 925, 1067
Alw21I GWGCWC 2 cut(s) 124, 249
Alw26I GTCTC 6 cut(s) 10, 109, 418, 855, 986, 1063
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 634
ApoI RAATTY 5 cut(s) 160, 681, 930, 940, 1266
AsiGI ACCGGT 2 cut(s) 206, 230
Asp700I GAANNNNTTC 1 cut(s) 596
Asp718I GGTACC 1 cut(s) 1274
AspLEI GCGC 1 cut(s) 1311
AspS9I GGNCC 3 cut(s) 204, 634, 1195
AsuHPI GGTGA 5 cut(s) 25, 84, 194, 293, 689
AvaI CYCGRG 1 cut(s) 340
AvaII GGWCC 2 cut(s) 204, 1195
BanI GGYRCC 1 cut(s) 1274
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
Bbv12I GWGCWC 2 cut(s) 124, 249
BccI CCATC 3 cut(s) 376, 645, 1207
BciVI GTATCC 1 cut(s) 1087
BclI TGATCA 2 cut(s) 865, 1033
BcoDI GTCTC 6 cut(s) 10, 109, 418, 855, 986, 1063
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 1 cut(s) 1260
BfuI GTATCC 1 cut(s) 1087
BglI GCCNNNNNGGC 1 cut(s) 1216
BglII AGATCT 2 cut(s) 142, 448
Bme18I GGWCC 2 cut(s) 204, 1195
BmeT110I CYCGRG 1 cut(s) 340
BmgT120I GGNCC 3 cut(s) 204, 634, 1195
BmiI GGNNCC 4 cut(s) 313, 1149, 1197, 1276
BmrI ACTGGG 1 cut(s) 1153
BmsI GCATC 1 cut(s) 470
BmuI ACTGGG 1 cut(s) 1153
BpuEI CTTGAG 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 1189
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 1 cut(s) 694
BsaWI WCCGGW 2 cut(s) 206, 230
Bse118I RCCGGY 2 cut(s) 206, 230
Bse1I ACTGG 2 cut(s) 989, 1148
Bse3DI GCAATG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 694
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 866, 1158
BseNI ACTGG 2 cut(s) 989, 1148
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 1311
BshFI GGCC 1 cut(s) 636
BshNI GGYRCC 1 cut(s) 1274
BshTI ACCGGT 2 cut(s) 206, 230
BsiHKAI GWGCWC 2 cut(s) 124, 249
BsiHKCI CYCGRG 1 cut(s) 340
BsiSI CCGG 2 cut(s) 207, 231
BslFI GGGAC 1 cut(s) 631
BsmAI GTCTC 6 cut(s) 10, 109, 418, 855, 986, 1063
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 631
BsnI GGCC 1 cut(s) 636
Bso31I GGTCTC 1 cut(s) 986
BsoBI CYCGRG 1 cut(s) 340
Bsp1286I GDGCHC 2 cut(s) 124, 249
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 2 cut(s) 865, 1159
BspFNI CGCG 1 cut(s) 1311
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 4 cut(s) 313, 1149, 1197, 1276
BspT107I GGYRCC 1 cut(s) 1274
BspTNI GGTCTC 1 cut(s) 986
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 2 cut(s) 206, 230
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 2 cut(s) 989, 1148
BssAI RCCGGY 2 cut(s) 206, 230
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
BssNI GRCGYC 1 cut(s) 1189
BssT1I CCWWGG 1 cut(s) 694
Bst4CI ACNGT 2 cut(s) 629, 1025
Bst6I CTCTTC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 1189
BstAUI TGTACA 1 cut(s) 624
BstC8I GCNNGC 2 cut(s) 217, 1016
BstDEI CTNAG 4 cut(s) 727, 852, 997, 1167
BstFNI CGCG 1 cut(s) 1311
BstHHI GCGC 1 cut(s) 1311
BstKTI GATC 7 cut(s) 145, 451, 583, 655, 868, 958, 1036
BstMAI GTCTC 6 cut(s) 10, 109, 418, 855, 986, 1063
BstMBI GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 195, 1216
BstNSI RCATGY 1 cut(s) 1255
BstSFI CTRYAG 1 cut(s) 1260
BstUI CGCG 1 cut(s) 1311
BstX2I RGATCY 2 cut(s) 142, 448
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 2 cut(s) 142, 448
BsuI GTATCC 1 cut(s) 1087
BsuRI GGCC 1 cut(s) 636
BtsIMutI CAGTG 2 cut(s) 860, 996
Cac8I GCNNGC 2 cut(s) 217, 1016
CciI TCATGA 1 cut(s) 12
CfoI GCGC 1 cut(s) 1311
Cfr10I RCCGGY 2 cut(s) 206, 230
Cfr13I GGNCC 3 cut(s) 204, 634, 1195
CseI GACGC 2 cut(s) 42, 1178
Csp6I GTAC 3 cut(s) 625, 1026, 1275
CspAI ACCGGT 2 cut(s) 206, 230
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 7 cut(s) 13, 236, 436, 618, 674, 1215, 1252
CviQI GTAC 3 cut(s) 625, 1026, 1275
DdeI CTNAG 4 cut(s) 727, 852, 997, 1167
DpnI GATC 7 cut(s) 144, 450, 582, 654, 867, 957, 1035
DpnII GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
DraI TTTAAA 1 cut(s) 937
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 986
Eco32I GATATC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 204, 1195
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 1083
Eco88I CYCGRG 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 247
EcoRV GATATC 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 694
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 7 cut(s) 16, 239, 439, 621, 677, 1218, 1255
FaqI GGGAC 1 cut(s) 631
FatI CATG 7 cut(s) 12, 235, 435, 617, 673, 1214, 1251
FbaI TGATCA 2 cut(s) 865, 1033
FriOI GRGCYC 1 cut(s) 249
FspBI CTAG 1 cut(s) 926
GlaI GCGC 1 cut(s) 1310
GsaI CCCAGC 1 cut(s) 299
HaeIII GGCC 1 cut(s) 636
HapII CCGG 2 cut(s) 207, 231
HgaI GACGC 2 cut(s) 42, 1178
HhaI GCGC 1 cut(s) 1311
Hin1I GRCGYC 1 cut(s) 1189
Hin1II CATG 7 cut(s) 16, 239, 439, 621, 677, 1218, 1255
Hin6I GCGC 1 cut(s) 1309
HinP1I GCGC 1 cut(s) 1309
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 5 cut(s) 9, 152, 391, 644, 1123
HpaII CCGG 2 cut(s) 207, 231
HphI GGTGA 5 cut(s) 25, 84, 194, 293, 689
Hpy166II GTNNAC 3 cut(s) 481, 786, 1257
Hpy188I TCNGA 6 cut(s) 589, 832, 1063, 1168, 1204, 1284
Hpy8I GTNNAC 3 cut(s) 481, 786, 1257
HpyAV CCTTC 2 cut(s) 179, 501
HpyCH4III ACNGT 2 cut(s) 629, 1025
HpyCH4V TGCA 6 cut(s) 75, 102, 198, 511, 1018, 1106
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 1216
HpyF3I CTNAG 4 cut(s) 727, 852, 997, 1167
Hsp92I GRCGYC 1 cut(s) 1189
Hsp92II CATG 7 cut(s) 16, 239, 439, 621, 677, 1218, 1255
HspAI GCGC 1 cut(s) 1309
KpnI GGTACC 1 cut(s) 1278
Ksp22I TGATCA 2 cut(s) 865, 1033
Kzo9I GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
LmnI GCTCC 1 cut(s) 252
LweI GCATC 1 cut(s) 470
MaeI CTAG 1 cut(s) 926
MaeIII GTNAC 1 cut(s) 471
MalI GATC 7 cut(s) 144, 450, 582, 654, 867, 957, 1035
MboI GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
MboII GAAGA 3 cut(s) 32, 147, 284
MfeI CAATTG 1 cut(s) 199
MflI RGATCY 2 cut(s) 142, 448
MhlI GDGCHC 2 cut(s) 124, 249
MlyI GAGTC 3 cut(s) 18, 653, 1117
MmeI TCCRAC 1 cut(s) 1307
MnlI CCTC 6 cut(s) 181, 330, 659, 816, 1042, 1161
MroXI GAANNNNTTC 1 cut(s) 596
MslI CAYNNNNRTG 2 cut(s) 240, 989
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 2 cut(s) 207, 231
MunI CAATTG 1 cut(s) 199
MvnI CGCG 1 cut(s) 1311
MwoI GCNNNNNNNGC 2 cut(s) 195, 1216
NdeII GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
NlaIII CATG 7 cut(s) 16, 239, 439, 621, 677, 1218, 1255
NlaIV GGNNCC 4 cut(s) 313, 1149, 1197, 1276
NspI RCATGY 1 cut(s) 1255
PaeR7I CTCGAG 1 cut(s) 340
PagI TCATGA 1 cut(s) 12
PciI ACATGT 1 cut(s) 1251
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 152, 391
PinAI ACCGGT 2 cut(s) 206, 230
PleI GAGTC 3 cut(s) 17, 652, 1117
PpsI GAGTC 3 cut(s) 17, 652, 1117
PscI ACATGT 1 cut(s) 1251
Psp124BI GAGCTC 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 295
PspN4I GGNNCC 4 cut(s) 313, 1149, 1197, 1276
PspPI GGNCC 3 cut(s) 204, 634, 1195
PsuI RGATCY 2 cut(s) 142, 448
RsaI GTAC 3 cut(s) 626, 1027, 1276
RsaNI GTAC 3 cut(s) 625, 1026, 1275
RseI CAYNNNNRTG 2 cut(s) 240, 989
SacI GAGCTC 1 cut(s) 249
Sau3AI GATC 7 cut(s) 142, 448, 580, 652, 865, 955, 1033
Sau96I GGNCC 3 cut(s) 204, 634, 1195
SchI GAGTC 3 cut(s) 18, 653, 1117
SduI GDGCHC 2 cut(s) 124, 249
SfaNI GCATC 1 cut(s) 470
SfcI CTRYAG 1 cut(s) 1260
Sfr274I CTCGAG 1 cut(s) 340
SinI GGWCC 2 cut(s) 204, 1195
SlaI CTCGAG 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 240, 989
SmlI CTYRAG 2 cut(s) 340, 641
SmoI CTYRAG 2 cut(s) 340, 641
SsiI CCGC 1 cut(s) 309
SspMI CTAG 1 cut(s) 926
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 2 cut(s) 629, 1025
TaqI TCGA 8 cut(s) 79, 341, 387, 396, 504, 651, 686, 1047
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 2 cut(s) 152, 391
TscAI CASTG 2 cut(s) 860, 996
TspDTI ATGAA 6 cut(s) 278, 285, 521, 662, 707, 1032
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 860, 996
VpaK11BI GGWCC 2 cut(s) 204, 1195
XapI RAATTY 5 cut(s) 160, 681, 930, 940, 1266
XceI RCATGY 1 cut(s) 1255
XhoI CTCGAG 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.