Rroxscaffold_4G00284370

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000281
Physical Location & Seq
Reverse (-)
6009918 .. 6012836
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284370.1

Sequence Viewer

Length: 2919 bp
ATGGGTAGGATCATGGGAGCGACCTTACTTCTCTGGTTTCTAATCACTGCAACCACTAGCATTAGCTTGTGTAATGGGAACCCTGGTGTGGCTTGCAAAGAAAGTGAAAGACTAGCACTTCTGATGTTGAAGCAAGATTTTACTGATCCTTCAAATCGGCTTTTATCATGGGCAGGTGAAGGAGACTGTTGCCATTGGACTGGAGTTGTCTGTGATAATGTAACCGGTCACATACGCGAGCTCCACCTTGGTAATTACTCTAATCTCAACTCTTCCTCTTACTTGGGAGGTAAGATAAATCCTTCTCTGCTCAATTTAACGCAACTAACCTACTTGGACCTGAGTAACAACTTTTTCCAAGGAATGCAGATCCCTAGCTTCTTTGGTTCTCTTAATAGTTTAACTCATCTTGACCTTTCAAGAAGCTTCGAAGGAATGGTTCCTCCTCAACTGGGGAATCTCTCGAATCTACGTGATCTACGTCTCAGTGGTTATGGTTTGAAGGCTGAGAACCTTCAATGGATTTCTGGTCTTTCTCAGTTGGAGCACCTGGACGCGAGTTTTCTTGATCTTAGCAAAGTATCTGATGATTGGCTGCCAGTGATACAGATGCGGCTCCCCTCTTTGGTAGAGTTGCATATGTCAGCTTGCAGTCTCCATTACATTCCCCATTTAACTATCATCAATTTTACTTCACTTGCCATCCTCGATCTTTCTTTCAACAGCTTTAATTCTTTCATGCCTAAGTGGGTTTTCAGTTTCAGAAATCTGGTGTCTCTTTCTCTTAGAGAATGTGGTTTCCAAGGTCCAATTCCTAGCAGTAGCAGCCCACACAATATCACATCTCTCAGGGAGATTGATCTCTCAATTAATTACCTTAGGGAGCCGATACCGAAATGGCTGCTTAACAACAAAGACCTTACTGTACTCGATCTAGGACACAATTCTTATACAGGACCAATTCCAGGTGGCATTGTGAATATGACTAGTCTTAAGGATCTTCATCTCGAGTATAATTCTTTCATCTCTACCATACCTGAATGGTTGTCTAGTTTTAATCATCTTGAATCCTTGGTTCTTTCTAACAATCACTTGGACGGTGAAATTTTGAGTTCCATTGGAAACTTGACATCCATTGTATATCTTGGTTTAGATCGTAATCATTTTGAAGGGACAATCCCAAACTCCTTGGGCAATCTGTCATCCTTAGAGTCGTTACAGATGTCATACAACTCGTTTCATGGAACTCTTCCAGAGACCATTGGCCAACTCAAAAGGCTAACAGCTTTGGATATATCTTTTAATTCATTTGAAGGTGTAGTGTCTGAAGTTCATTTTACACATCTTACAAGTTTAAAATCCTTCAGAGCACATGGAAACTCATTGACTCTAAAAACTAGTCGAGATTGGCTTCCTCCTTTTCAACTTCAAAGCTTGTATTTAGATTCTTGGCATTTGGGGCTTGAACTGCCTTCATGGCTTCGAACACAAACACAATTGCTGGAGCTAAGCCTATCGGGTACAGGAATTTCAGGTACCATTCCGAATTGGTGTTGGAACTTTTCTTCCGCACTAGTTTTCCTGGATCTGTCCAACAATCAATTACATGGGGAGATTCAATATATAAATGTTGGCGCCCCATTCTCAATAATTGACCTAAGTTCAAACCAGTTCAACGGTTCATTGCCTCTTGTCTCCTCCACTATAGAACTACTAGATCTTTCGAATTCTTCATTTTCTGGATCTCTGTTCCACTTCTTTTGTGCTAGGATGGACATACCAAAAGCGCTCTATTTTCTTCATCTAGGGAACAATCATCTCAGTGGTGAAATTCCTGATTGTTGGATGAATTGGCTTAATTTGCAAGCCATAACATTAGGTAACAACAATTTGACTGGAAAAATTCCAAGCTCCATTGGACACTTGCACATTCTGGAATCATTGCACTTGCGCAACAATCACCTTTCTGGAGAACTACCTCAATCCTTGCAGAACTGTACACAATTGTTAGTAGTTGACCTTGGTAAAAATAAGCTCTCTGGAGGCTTGCCTATATGGATGGGGAAAAGCTTTCCAAACTTGGTGGTTCTTAGCCTTCGGTCAAATATGTTTCAAGGAGTCATTTCGGATCAACTTTGTAATATAAGCCGTCTCCTAATCTTGGACTTGGCAGATAATGTTCTCTCAGGAACAATACCGAGATGCTTCGGCAACTTTAGTGGAATTGCAAACTCGTCAATGTCAGATGCTTTCGCTACTTATGTTGGTAACTACAGACTGTACAGAGATAATACGTTCCTGGTGCAAAAAGGGAGAGAAGTGGAATTCGGAAAAATTCTTGGAATTGTAGCAAGCGTAGATCTTTCTAACAACAATTTATCTGGGGATATTCCTGAGGAACTGACAAATCTCCTCAGTTTGCAAACATTGAATTTATCAGCAAATCTTTTGACCGGAAGAATCCCTTCGATGATTGGCAGATTGAGGCAGTTAGAATCATTTGATTTGTCTACAAACCAACTTTCTGGTGAAATTCCTCCAAGCATAACGAATTTGACGTTTCTGAGTCACTTGAACTTGTCTCACAACAGTCTGACGGGACAGATTCCACAAAGCACTCAGCTTCAGAGTCTTGATGAGTCCAGCTTTGTCGGCAATGAACTTTGCGGACCTCCACTCAGCAAGAATTGCAGTGCAAGCAAGGCGATGCCACCAACACTTGATCGGCAAGACAAAGGATATGATTTACTGGAAGATGAGTGGTTCTACCTGAGCTTGGGATTGGGATTCATGGTTGGTTTTTGGTGTATACTTGGTTCTTTGCTGGTAAACATGCCATGGAGCTTTGCTTTTTCACGATTCCTCAATAGCATTGTGCTTAAACTTTACGCTGTAATTGTTATTTTTTTTGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

972

Amino Acids

107.69

Weight (kDa)

5.6

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 72 1.2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 98 - 262 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 282 - 340 8.3e-08 Leucine rich repeat
LRR_14 PF23598 305 - 504 1e-17 Leucine-rich repeat region
LRR_8 PF13855 380 - 436 1.3e-06 Leucine rich repeat
LRR_8 PF13855 597 - 655 2.6e-06 Leucine rich repeat
LRR_8 PF13855 784 - 841 6.1e-06 Leucine rich repeat
LRR_14 PF23598 798 - 880 3.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc16I TGCGCA 1 cut(s) 1952
Acc36I ACCTGC 1 cut(s) 164
Acc65I GGTACC 1 cut(s) 1535
AccB1I GGYRCC 2 cut(s) 1535, 1634
AccI GTMKAC 2 cut(s) 2510, 2809
AccII CGCG 2 cut(s) 237, 557
AciI CCGC 3 cut(s) 613, 1569, 2667
AclWI GGATC 7 cut(s) 17, 140, 364, 1005, 1593, 1750, 2136
AcoI YGGCCR 1 cut(s) 1264
AcuI CTGAAG 3 cut(s) 1347, 1348, 2609
AcyI GRCGYC 1 cut(s) 1635
AfaI GTAC 5 cut(s) 927, 1522, 1537, 1999, 2282
AfeI AGCGCT 1 cut(s) 1788
AfiI CCNNNNNNNGG 6 cut(s) 88, 452, 625, 965, 2161, 2776
AflII CTTAAG 1 cut(s) 992
AgeI ACCGGT 1 cut(s) 224
AhlI ACTAGT 3 cut(s) 986, 1397, 1573
AjnI CCWGG 5 cut(s) 82, 549, 964, 1581, 2298
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AloI GAACNNNNNNTCC 2 cut(s) 1550, 1582
Alw21I GWGCWC 3 cut(s) 243, 549, 1372
Alw26I GTCTC 8 cut(s) 177, 488, 659, 780, 1250, 1699, 2156, 2586
AlwI GGATC 7 cut(s) 17, 140, 364, 1005, 1593, 1750, 2136
Ama87I CYCGRG 1 cut(s) 1007
Aor51HI AGCGCT 1 cut(s) 1788
AoxI GGCC 1 cut(s) 1264
ApeKI GCWGC 3 cut(s) 595, 825, 901
AseI ATTAAT 1 cut(s) 870
AsiGI ACCGGT 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 2464
Asp718I GGTACC 1 cut(s) 1535
AspLEI GCGC 3 cut(s) 1637, 1789, 1953
AspS9I GGNCC 4 cut(s) 337, 806, 956, 2669
AsuHPI GGTGA 5 cut(s) 188, 1112, 1838, 1952, 2540
AsuII TTCGAA 3 cut(s) 429, 1483, 1724
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 4 cut(s) 337, 806, 956, 2669
AxyI CCTNAGG 2 cut(s) 878, 2394
BalI TGGCCA 1 cut(s) 1266
BanI GGYRCC 2 cut(s) 1535, 1634
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 3 cut(s) 243, 549, 1372
BbvI GCAGC 3 cut(s) 582, 837, 888
BccI CCATC 3 cut(s) 710, 1765, 2053
BceAI ACGGC 1 cut(s) 2133
BcgI CGANNNNNNTGC 2 cut(s) 883, 917
BciT130I CCWGG 5 cut(s) 84, 551, 966, 1583, 2300
BcoDI GTCTC 8 cut(s) 177, 488, 659, 780, 1250, 1699, 2156, 2586
BcuI ACTAGT 3 cut(s) 986, 1397, 1573
BfmI CTRYAG 2 cut(s) 1704, 2272
BfoI RGCGCY 2 cut(s) 1638, 1790
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 1477
BglII AGATCT 2 cut(s) 1717, 2359
BisI GCNGC 4 cut(s) 596, 614, 826, 902
BlpI GCTNAGC 1 cut(s) 1508
BlsI GCNGC 4 cut(s) 597, 615, 827, 903
Bme1390I CCNGG 5 cut(s) 84, 551, 966, 1583, 2300
Bme18I GGWCC 4 cut(s) 337, 806, 956, 2669
BmeT110I CYCGRG 1 cut(s) 1007
BmgT120I GGNCC 4 cut(s) 337, 806, 956, 2669
BmiI GGNNCC 6 cut(s) 80, 441, 617, 885, 1537, 1636
BmrFI CCNGG 5 cut(s) 84, 551, 966, 1583, 2300
BmrI ACTGGG 1 cut(s) 461
BmsI GCATC 4 cut(s) 600, 2192, 2236, 2697
BmuI ACTGGG 1 cut(s) 461
BpmI CTGGAG 4 cut(s) 222, 1523, 1989, 2061
Bpu10I CCTNAGC 1 cut(s) 2771
Bpu1102I GCTNAGC 1 cut(s) 1508
Bpu14I TTCGAA 3 cut(s) 429, 1483, 1724
BsaAI YACGTR 1 cut(s) 473
BsaBI GATNNNNATC 2 cut(s) 1002, 1158
BsaHI GRCGYC 1 cut(s) 1635
BsaI GGTCTC 1 cut(s) 1250
BsaJI CCNNGG 8 cut(s) 82, 247, 358, 802, 1071, 1188, 2020, 2837
BsaWI WCCGGW 2 cut(s) 224, 2453
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 452, 625, 965, 2161, 2776
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 6 cut(s) 205, 456, 599, 1669, 1900, 2754
Bse21I CCTNAGG 2 cut(s) 878, 2394
Bse3DI GCAATG 3 cut(s) 1682, 1940, 2662
Bse8I GATNNNNATC 2 cut(s) 1002, 1158
BseBI CCWGG 5 cut(s) 84, 551, 966, 1583, 2300
BseDI CCNNGG 8 cut(s) 82, 247, 358, 802, 1071, 1188, 2020, 2837
BseGI GGATG 6 cut(s) 702, 1130, 1202, 1776, 1851, 2064
BseJI GATNNNNATC 2 cut(s) 1002, 1158
BseLI CCNNNNNNNGG 6 cut(s) 88, 452, 625, 965, 2161, 2776
BseMI GCAATG 3 cut(s) 1682, 1940, 2662
BseNI ACTGG 6 cut(s) 205, 456, 599, 1669, 1900, 2754
BseRI GAGGAG 3 cut(s) 435, 1687, 2402
BseXI GCAGC 3 cut(s) 582, 837, 888
Bsh1236I CGCG 2 cut(s) 237, 557
BshFI GGCC 1 cut(s) 1266
BshNI GGYRCC 2 cut(s) 1535, 1634
BshTI ACCGGT 1 cut(s) 224
BsiHKAI GWGCWC 3 cut(s) 243, 549, 1372
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 2 cut(s) 225, 2454
BslFI GGGAC 2 cut(s) 1186, 2613
BslI CCNNNNNNNGG 6 cut(s) 88, 452, 625, 965, 2161, 2776
BsmAI GTCTC 8 cut(s) 177, 488, 659, 780, 1250, 1699, 2156, 2586
BsmBI CGTCTC 2 cut(s) 488, 2156
BsmFI GGGAC 2 cut(s) 1186, 2613
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 1 cut(s) 1266
Bso31I GGTCTC 1 cut(s) 1250
BsoBI CYCGRG 1 cut(s) 1007
Bsp119I TTCGAA 3 cut(s) 429, 1483, 1724
Bsp1286I GDGCHC 3 cut(s) 243, 549, 1372
Bsp1407I TGTACA 2 cut(s) 1997, 2280
Bsp1720I GCTNAGC 1 cut(s) 1508
Bsp19I CCATGG 1 cut(s) 2837
BspACI CCGC 3 cut(s) 613, 1569, 2667
BspANI GGCC 1 cut(s) 1266
BspFNI CGCG 2 cut(s) 237, 557
BspLI GGNNCC 6 cut(s) 80, 441, 617, 885, 1537, 1636
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 7 cut(s) 17, 140, 364, 1005, 1593, 1750, 2136
BspT104I TTCGAA 3 cut(s) 429, 1483, 1724
BspT107I GGYRCC 2 cut(s) 1535, 1634
BspTI CTTAAG 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 1250
BsrDI GCAATG 3 cut(s) 1682, 1940, 2662
BsrFI RCCGGY 1 cut(s) 224
BsrGI TGTACA 2 cut(s) 1997, 2280
BsrI ACTGG 6 cut(s) 205, 456, 599, 1669, 1900, 2754
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 8 cut(s) 82, 247, 358, 802, 1071, 1188, 2020, 2837
BssNAI GTATAC 1 cut(s) 2810
BssNI GRCGYC 1 cut(s) 1635
BssT1I CCWWGG 7 cut(s) 247, 358, 802, 1071, 1188, 2020, 2837
Bst1107I GTATAC 1 cut(s) 2810
Bst2UI CCWGG 5 cut(s) 84, 551, 966, 1583, 2300
Bst4CI ACNGT 7 cut(s) 188, 925, 1100, 1679, 1997, 2280, 2591
Bst6I CTCTTC 2 cut(s) 277, 1254
BstACI GRCGYC 1 cut(s) 1635
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 1 cut(s) 2688
BstAUI TGTACA 2 cut(s) 1997, 2280
BstBAI YACGTR 1 cut(s) 473
BstBI TTCGAA 3 cut(s) 429, 1483, 1724
BstC8I GCNNGC 7 cut(s) 94, 239, 649, 1866, 2048, 2353, 2698
BstDSI CCRYGG 1 cut(s) 2837
BstF5I GGATG 6 cut(s) 702, 1130, 1202, 1776, 1851, 2064
BstFNI CGCG 2 cut(s) 237, 557
BstH2I RGCGCY 2 cut(s) 1638, 1790
BstHHI GCGC 3 cut(s) 1637, 1789, 1953
BstMAI GTCTC 8 cut(s) 177, 488, 659, 780, 1250, 1699, 2156, 2586
BstMWI GCNNNNNNNGC 9 cut(s) 825, 1459, 1468, 1477, 1861, 2652, 2688, 2697, 2702
BstNI CCWGG 5 cut(s) 84, 551, 966, 1583, 2300
BstNSI RCATGY 1 cut(s) 2836
BstSCI CCNGG 5 cut(s) 82, 549, 964, 1581, 2298
BstSFI CTRYAG 2 cut(s) 1704, 2272
BstUI CGCG 2 cut(s) 237, 557
BstV1I GCAGC 3 cut(s) 582, 837, 888
BstX2I RGATCY 6 cut(s) 369, 997, 1585, 1717, 1742, 2359
BstXI CCANNNNNNTGG 2 cut(s) 200, 2525
BstYI RGATCY 6 cut(s) 369, 997, 1585, 1717, 1742, 2359
BstZ17I GTATAC 1 cut(s) 2810
Bsu36I CCTNAGG 2 cut(s) 878, 2394
BsuRI GGCC 1 cut(s) 1266
BtgI CCRYGG 1 cut(s) 2837
BtgZI GCGATG 1 cut(s) 2720
BtsCI GGATG 6 cut(s) 702, 1130, 1202, 1776, 1851, 2064
BtsI GCAGTG 2 cut(s) 45, 2698
BtsIMutI CAGTG 5 cut(s) 45, 493, 606, 1828, 2698
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 7 cut(s) 94, 239, 649, 1866, 2048, 2353, 2698
CfoI GCGC 3 cut(s) 1637, 1789, 1953
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 4 cut(s) 337, 806, 956, 2669
CseI GACGC 1 cut(s) 563
Csp6I GTAC 5 cut(s) 926, 1521, 1536, 1998, 2281
CspAI ACCGGT 1 cut(s) 224
CspCI CAANNNNNGTGG 6 cut(s) 1742, 1777, 2064, 2099, 2200, 2235
CviQI GTAC 5 cut(s) 926, 1521, 1536, 1998, 2281
DinI GGCGCC 1 cut(s) 1636
DraI TTTAAA 1 cut(s) 1356
EaeI YGGCCR 1 cut(s) 1264
Eam1104I CTCTTC 2 cut(s) 277, 1254
EarI CTCTTC 2 cut(s) 277, 1254
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 7 cut(s) 247, 358, 802, 1071, 1188, 2020, 2837
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 1250
Eco47I GGWCC 4 cut(s) 337, 806, 956, 2669
Eco47III AGCGCT 1 cut(s) 1788
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 3 cut(s) 1347, 1348, 2609
Eco81I CCTNAGG 2 cut(s) 878, 2394
Eco88I CYCGRG 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 241
EcoRI GAATTC 2 cut(s) 1726, 2324
EcoRII CCWGG 5 cut(s) 82, 549, 964, 1581, 2298
EcoT14I CCWWGG 7 cut(s) 247, 358, 802, 1071, 1188, 2020, 2837
EcoT38I GRGCYC 1 cut(s) 243
EgeI GGCGCC 1 cut(s) 1636
EheI GGCGCC 1 cut(s) 1636
ErhI CCWWGG 7 cut(s) 247, 358, 802, 1071, 1188, 2020, 2837
Esp3I CGTCTC 2 cut(s) 488, 2156
FalI AAGNNNNNCTT 2 cut(s) 2449, 2481
FaqI GGGAC 2 cut(s) 1186, 2613
FauNDI CATATG 1 cut(s) 639
FblI GTMKAC 2 cut(s) 2510, 2809
Fnu4HI GCNGC 4 cut(s) 596, 614, 826, 902
FokI GGATG 6 cut(s) 689, 1117, 1189, 1783, 1858, 2071
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 4 cut(s) 596, 614, 826, 902
FspI TGCGCA 1 cut(s) 1952
GlaI GCGC 3 cut(s) 1636, 1788, 1952
GluI GCNGC 4 cut(s) 596, 614, 826, 902
GsuI CTGGAG 4 cut(s) 222, 1523, 1989, 2061
HaeII RGCGCY 2 cut(s) 1638, 1790
HaeIII GGCC 1 cut(s) 1266
HapII CCGG 2 cut(s) 225, 2454
HgaI GACGC 1 cut(s) 563
HhaI GCGC 3 cut(s) 1637, 1789, 1953
Hin1I GRCGYC 1 cut(s) 1635
Hin6I GCGC 3 cut(s) 1635, 1787, 1951
HinP1I GCGC 3 cut(s) 1635, 1787, 1951
HincII GTYRAC 1 cut(s) 2017
HindII GTYRAC 1 cut(s) 2017
HindIII AAGCTT 3 cut(s) 424, 1432, 2068
HpaII CCGG 2 cut(s) 225, 2454
HphI GGTGA 5 cut(s) 188, 1112, 1838, 1952, 2540
Hpy166II GTNNAC 5 cut(s) 2000, 2017, 2511, 2810, 2830
Hpy8I GTNNAC 5 cut(s) 2000, 2017, 2511, 2810, 2830
HpyCH4III ACNGT 7 cut(s) 188, 925, 1100, 1679, 1997, 2280, 2591
HpyCH4IV ACGT 4 cut(s) 472, 481, 2294, 2558
HpyF10VI GCNNNNNNNGC 9 cut(s) 825, 1459, 1468, 1477, 1861, 2652, 2688, 2697, 2702
HpySE526I ACGT 4 cut(s) 472, 481, 2294, 2558
Hsp92I GRCGYC 1 cut(s) 1635
HspAI GCGC 3 cut(s) 1635, 1787, 1951
KasI GGCGCC 1 cut(s) 1634
KpnI GGTACC 1 cut(s) 1539
LmnI GCTCC 8 cut(s) 17, 246, 544, 621, 883, 1504, 1916, 2841
Lsp1109I GCAGC 3 cut(s) 582, 837, 888
LweI GCATC 4 cut(s) 600, 2192, 2236, 2697
MaeII ACGT 4 cut(s) 472, 481, 2294, 2558
MaeIII GTNAC 7 cut(s) 220, 227, 344, 1215, 1880, 2267, 2567
MboII GAAGA 8 cut(s) 264, 992, 1241, 1557, 1722, 1790, 2469, 2765
MfeI CAATTG 2 cut(s) 1496, 2003
MflI RGATCY 6 cut(s) 369, 997, 1585, 1717, 1742, 2359
MhlI GDGCHC 3 cut(s) 243, 549, 1372
MlsI TGGCCA 1 cut(s) 1266
MluNI TGGCCA 1 cut(s) 1266
Mly113I GGCGCC 1 cut(s) 1635
MlyI GAGTC 6 cut(s) 1220, 1381, 2127, 2575, 2638, 2648
MmeI TCCRAC 4 cut(s) 522, 1535, 1617, 1823
Mox20I TGGCCA 1 cut(s) 1266
MroXI GAANNNNTTC 1 cut(s) 2464
MscI TGGCCA 1 cut(s) 1266
MslI CAYNNNNRTG 1 cut(s) 1821
Msp20I TGGCCA 1 cut(s) 1266
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 2 cut(s) 225, 2454
MspR9I CCNGG 5 cut(s) 84, 551, 966, 1583, 2300
MunI CAATTG 2 cut(s) 1496, 2003
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 5 cut(s) 84, 551, 966, 1583, 2300
MvnI CGCG 2 cut(s) 237, 557
MwoI GCNNNNNNNGC 9 cut(s) 825, 1459, 1468, 1477, 1861, 2652, 2688, 2697, 2702
NarI GGCGCC 1 cut(s) 1635
NcoI CCATGG 1 cut(s) 2837
NdeI CATATG 1 cut(s) 639
NlaIV GGNNCC 6 cut(s) 80, 441, 617, 885, 1537, 1636
NmuCI GTSAC 2 cut(s) 227, 2567
NsbI TGCGCA 1 cut(s) 1952
NspI RCATGY 1 cut(s) 2836
NspV TTCGAA 3 cut(s) 429, 1483, 1724
PaeR7I CTCGAG 1 cut(s) 1007
PaqCI CACCTGC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 478, 2555
PctI GAATGC 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 2464
PfoI TCCNGGA 1 cut(s) 1581
PinAI ACCGGT 1 cut(s) 224
PkrI GCNGC 4 cut(s) 597, 615, 827, 903
PleI GAGTC 6 cut(s) 1219, 1381, 2126, 2574, 2637, 2647
PluTI GGCGCC 1 cut(s) 1638
PpsI GAGTC 6 cut(s) 1219, 1381, 2126, 2574, 2637, 2647
Ppu21I YACGTR 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 870
Psp124BI GAGCTC 1 cut(s) 243
Psp6I CCWGG 5 cut(s) 82, 549, 964, 1581, 2298
PspGI CCWGG 5 cut(s) 82, 549, 964, 1581, 2298
PspN4I GGNNCC 6 cut(s) 80, 441, 617, 885, 1537, 1636
PspPI GGNCC 4 cut(s) 337, 806, 956, 2669
PsrI GAACNNNNNNTAC 2 cut(s) 2800, 2832
PsuI RGATCY 6 cut(s) 369, 997, 1585, 1717, 1742, 2359
RsaI GTAC 5 cut(s) 927, 1522, 1537, 1999, 2282
RsaNI GTAC 5 cut(s) 926, 1521, 1536, 1998, 2281
RseI CAYNNNNRTG 1 cut(s) 1821
SacI GAGCTC 1 cut(s) 243
SatI GCNGC 4 cut(s) 596, 614, 826, 902
Sau96I GGNCC 4 cut(s) 337, 806, 956, 2669
SchI GAGTC 6 cut(s) 1220, 1381, 2127, 2575, 2638, 2648
ScrFI CCNGG 5 cut(s) 84, 551, 966, 1583, 2300
SduI GDGCHC 3 cut(s) 243, 549, 1372
SfaNI GCATC 4 cut(s) 600, 2192, 2236, 2697
SfcI CTRYAG 2 cut(s) 1704, 2272
SfoI GGCGCC 1 cut(s) 1636
Sfr274I CTCGAG 1 cut(s) 1007
SfuI TTCGAA 3 cut(s) 429, 1483, 1724
SinI GGWCC 4 cut(s) 337, 806, 956, 2669
SlaI CTCGAG 1 cut(s) 1007
SmiMI CAYNNNNRTG 1 cut(s) 1821
SmlI CTYRAG 2 cut(s) 992, 1007
SmoI CTYRAG 2 cut(s) 992, 1007
SpeI ACTAGT 3 cut(s) 986, 1397, 1573
SsiI CCGC 3 cut(s) 613, 1569, 2667
SspDI GGCGCC 1 cut(s) 1634
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 82, 549, 964, 1581, 2298
StyI CCWWGG 7 cut(s) 247, 358, 802, 1071, 1188, 2020, 2837
TaaI ACNGT 7 cut(s) 188, 925, 1100, 1679, 1997, 2280, 2591
TaiI ACGT 4 cut(s) 475, 484, 2297, 2561
TaqI TCGA 9 cut(s) 429, 464, 708, 930, 1008, 1402, 1483, 1724, 2468
TaqII GACCGA 1 cut(s) 2088
TatI WGTACW 3 cut(s) 925, 1997, 2280
TauI GCSGC 1 cut(s) 616
TscAI CASTG 5 cut(s) 52, 493, 606, 1828, 2698
TseFI GTSAC 2 cut(s) 227, 2567
TseI GCWGC 3 cut(s) 595, 825, 901
Tsp45I GTSAC 2 cut(s) 227, 2567
TspRI CASTG 5 cut(s) 52, 493, 606, 1828, 2698
Vha464I CTTAAG 1 cut(s) 992
VpaK11BI GGWCC 4 cut(s) 337, 806, 956, 2669
VspI ATTAAT 1 cut(s) 870
XceI RCATGY 1 cut(s) 2836
XhoI CTCGAG 1 cut(s) 1007
XmiI GTMKAC 2 cut(s) 2510, 2809
XmnI GAANNNNTTC 1 cut(s) 2464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.