FvH4_7g26566

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000281
Physical Location & Seq
Forward (+)
20049054 .. 20052473
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26566.t1

Sequence Viewer

Length: 3141 bp
ATGGAGAGATCCATGAGAATAGTCTTACTTCTCAGGCTCTTCACTATTGCAACCCTTACAATCAGTATGGGTTCTTGCAATGGAAACCTGGCTGTGCTTTGTAAAGAAAGTGAAAGAGATGCCCTTTTGAAGTTCAGGCAAGACCTCAAGGACCCTGCAAATCGGCTTCTATCGTGGGGCGGTGGAGGAGACTGTTGCAAATGGACTGGAGTGGTCTGTGATAACATAACCGGCCATATCCTTGAGCTCCATCTCAGTAAGCCTGATTCTGTTTGGGTGGAAGTCAACCCTATGTTGGGCGGTAAGATAAATCCTTCCTTGCTCATCTTAAAGCATCTGAGATACTTAGACCTTAGTAAAAATGATTTTCAAGGAATTCAGATTCCTGCATTCTTGGGTTCTCTTAAAAGTTTAAGATATCTTAACCTCTCCTACGCTGGGTTCGAGGGAACAATCCCTCATCAACTAGGAAATCTCTCCAGTCTACGCGACCTACGTCTCGATAATTATAATGGTGTGAATATAGTTGAGAGCCTTCACTGGATATCTGGTCTTTCTCAGTTGGAATACCTGGACATGAGTCGTGTAAATCTTAGCAAAGCATCAGATCATTGGTTGCAAGTCACAAACATGCTCCCCTCTTTGGTAGAGTTGCATTTGTCCCAATGTGGACTTGTTAATATTCCACCTCTACCTATCACAAATTTCACTTCACTTGCCATCCTTGATCTTTCTCTCAATTCCTTCAACTCTTTGATCCCCAGGTGGTTTTTAAGTCTTCGAAATCTAGCTTCTCTTGATCTTTTTGGATGTGGTTTCCAAGGTCCTATTCCTGGCAGTCCTCACAATATCACATCTCTTAGGGAAATTGATTTGTCACAGAATAGTCTCACACTTCCAATACCTGAGTGGCTGTTTAACCACAGGGAACTCACTCTTTTGAGACTAGGATACAATCAATTTGGAGGGCCGTTTCCCGGTGGTATTGTGAATATGACTGGTCTTAAAACTCTTCATCTGGAATCGAACAATTTCCATTCTTCCATACCTAAATGGTTGTATAGCCTTAAAAATCTTGAATCCATACGTCTTTCCTCCAATAAGTTGGATGGTGAAATTCCGAGTGACATTGGAAACTTGACATCCATTGCTGAGCTAAACTTAGATGATAATATGTTGAAAGGGAAGATCCCAAACACATTGGGATATCTTCGTGAGTTGATTTCCCTTGATCTGTCCTCAAACAACTTTAATGGAAATGTATCAGAATTCTTTGAGAGTTCACATCAAATCGACTATCTGTCACTGAAACATAATAATTTTTCAGGTCATTTGACTGAGCAGATAGGAAAGTGTAAGAATCTAAGCTACCTTGATCTTTCTTTTAATTCAATATCAGGTCCAATTCCAGTGTCCATAGGAAATCTGTCATCCATGATGAACTTAGACATTTCTCACAATCAGTTCAATGGAACTCTTCCAGAAAGTATAGGGCAACTCAAAATGATAACAGAATTGGAAATTTCTTATAATTCATTAGAAGGTGTAGTGTCAGAAGTTCATTTTGCTCATCTTGCAAGCTTGGAGTTCTTCAATGCTAATGCAAACTCCTTGACTCTGAAAACTCGTCGAGGCTGGTTTCCTCCTTTTAGTCTTCAGAGGTTGGTCCTAGATTATTGGCATATAGGACCTGAATTTCCCTTGTGGCTTCAAAGACAGACTAGATTGCAGTTTTTAAGCCTATCCAACACAGGAATTTCAGATACCATTCCGACTTGGTTTTGGAACTTTTCTTCCCAATTATATTACATGAATCTCTCACAGAATCAATTGTATGGAGAGATTCAAAATATAGATGCTGCACAAGGGTCAACAGTTGACCTTAGTTTTAACCAGTTCAATGGTTCATTACCCCTAGTCTCCTCAGCAATGGATTTACTGGATCTCTCCAATTCATCTTTTTCTGGTTCTGTCTTCCATTTCTTTTGTGATAAGATCGATATACCAAAGCGAGTTTCATATCTGCTTCTTGGAAACAATCTTCTCAGTGGAAACATTCCTGATTGTTGGAAGAACTGGAGATTTTTGGAAGTGCTGAATTTAGAGAACAACAATTTATCTGGAAACATTTCGAGCTCCTTGGGTTCCTTGAACCTCCGATCATTGCACCTGCGCAATAATTCTCTATCTGGAGAGTTACCTCCATCTCTGATGTATTCCATGAATATGAGGGTTCTTGACCTTGGTGAAAATAAGTTTGTGGGAAGCTTACCAGCAGCAATGATAGAAAATGGCTATTCAAGTTTGGTGGTTCTTAACCTTCGTTCGAATAAGCTACATGGAGACATTCCTGATGAACTCTGTGCTCTCAATAACCTCCAAATCTTGGACCTTGCCGATAACAATCTCTCAGGTACAATACCAAGATGCTTTCAGAATTTCAGCGCCATGGTCGTAGTGCCAGTATCAGATGGTTCCATTGAATGGTTTGATATTGTCGATTATGGAAGGTACATTGATAGCTCATTCCTAGTGACAAAAGGAAGAGAAGTGGAATACAGTAAGATCCTTGGATGGGTAACAAGCATGGACCTTTCAGCCAACGCTATATCTGGGGAGATGCCGGAGGAACTGACCAGTCTCATCAGCTTGCAAAATTTGAATATATCCGGTAATCTTCTGACCGGTAGAATTCCTACAAAGATCGGTAATATGGGACGACTAGAATCTCTTGATTTGTCTAAGAACCATCTTTTTGGTGTAATTCCTGCAAGCATGACAAGGATGACATTTCTGAATCACTTGAATGTGTCTTACAACAATCTGACGGGACGGATTCCAGAAAGCACTCAGCTTCAGAGCCTTGATCAGTCCGGCTTTGTTGGCAATGAACTCTGCGGACCTCCTCTCATCAAGAATTGCAGTGCAAGCAAGGTGATACCATTACCACCAACAGTTGAGCAAGAGAGAGGATATGATTTTCTTGAAGACAAGTGGTTCTATTTGAGCTTGGGATTGGGATTCGCAGTTGGTTTCTGGACTATACTTGGTTCCTTACTGGTAAACTTGCCATGGAGCTTTGCCTTTTCACGGTACCTCAATAGCATTGTGCTTAAACTTTATGCTGTAATTAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

1047

Amino Acids

116.61

Weight (kDa)

5.62

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 74 4.3e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 109 - 215 8.8e-07 Leucine-rich repeat region
LRR_8 PF13855 112 - 169 2.2e-07 Leucine rich repeat
LRR_8 PF13855 213 - 271 5.1e-09 Leucine rich repeat
LRR_14 PF23598 323 - 420 9.1e-07 Leucine-rich repeat region
LRR_8 PF13855 334 - 393 5.9e-08 Leucine rich repeat
LRR_14 PF23598 418 - 510 4.7e-08 Leucine-rich repeat region
LRR_8 PF13855 478 - 538 3.7e-06 Leucine rich repeat
LRR_8 PF13855 742 - 803 6.7e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 510, 1530
AarI CACCTGC 1 cut(s) 2178
Acc16I TGCGCA 1 cut(s) 2174
Acc36I ACCTGC 1 cut(s) 2178
Acc65I GGTACC 1 cut(s) 3093
AccB1I GGYRCC 1 cut(s) 3093
AccB7I CCANNNNNTGG 2 cut(s) 2386, 2484
AccI GTMKAC 1 cut(s) 484
AccII CGCG 1 cut(s) 489
AciI CCGC 3 cut(s) 180, 300, 2898
AclWI GGATC 5 cut(s) 3, 751, 1183, 1950, 2560
AcoI YGGCCR 1 cut(s) 232
AcuI CTGAAG 2 cut(s) 1640, 2840
AfaI GTAC 3 cut(s) 2416, 2513, 3095
AgeI ACCGGT 1 cut(s) 2684
AhdI GACNNNNNGTC 1 cut(s) 1300
AjnI CCWGG 4 cut(s) 87, 570, 761, 832
AjuI GAANNNNNNNTTGG 4 cut(s) 813, 845, 1999, 2031
AloI GAACNNNNNNTCC 2 cut(s) 1778, 1810
Alw21I GWGCWC 3 cut(s) 249, 2138, 2368
Alw26I GTCTC 7 cut(s) 183, 503, 893, 937, 1924, 2337, 2645
AlwI GGATC 5 cut(s) 3, 751, 1183, 1950, 2560
AoxI GGCC 2 cut(s) 232, 968
ApeKI GCWGC 2 cut(s) 1859, 2276
AseI ATTAAT 1 cut(s) 3132
AsiGI ACCGGT 1 cut(s) 2684
Asp700I GAANNNNTTC 2 cut(s) 1031, 2128
Asp718I GGTACC 1 cut(s) 3093
AspLEI GCGC 2 cut(s) 2175, 2447
AspS9I GGNCC 9 cut(s) 151, 824, 968, 1400, 1666, 1688, 2389, 2590, 2900
AsuC2I CCSGG 1 cut(s) 978
AsuHPI GGTGA 3 cut(s) 1124, 2258, 2947
AsuII TTCGAA 2 cut(s) 781, 2327
AvaII GGWCC 8 cut(s) 151, 824, 1400, 1666, 1688, 2389, 2590, 2900
BanI GGYRCC 1 cut(s) 3093
BanII GRGCYC 2 cut(s) 249, 2138
BbsI GAAGAC 4 cut(s) 770, 1646, 1966, 2994
Bbv12I GWGCWC 3 cut(s) 249, 2138, 2368
BbvCI CCTCAGC 1 cut(s) 1924
BbvI GCAGC 2 cut(s) 1846, 2288
BccI CCATC 7 cut(s) 258, 728, 1103, 2212, 2465, 2568, 2757
BceAI ACGGC 1 cut(s) 955
BciT130I CCWGG 4 cut(s) 89, 572, 763, 834
BciVI GTATCC 1 cut(s) 944
BclI TGATCA 1 cut(s) 2866
BcnI CCSGG 1 cut(s) 978
BcoDI GTCTC 7 cut(s) 183, 503, 893, 937, 1924, 2337, 2645
BfaI CTAG 8 cut(s) 467, 788, 947, 1670, 1722, 1916, 2531, 2723
BfoI RGCGCY 1 cut(s) 2448
BfuAI ACCTGC 1 cut(s) 2178
BfuI GTATCC 1 cut(s) 944
BisI GCNGC 2 cut(s) 1860, 2277
BlpI GCTNAGC 1 cut(s) 1152
BlsI GCNGC 2 cut(s) 1861, 2278
Bme1390I CCNGG 5 cut(s) 89, 572, 763, 834, 978
Bme18I GGWCC 8 cut(s) 151, 824, 1400, 1666, 1688, 2389, 2590, 2900
BmeRI GACNNNNNGTC 1 cut(s) 1300
BmgT120I GGNCC 9 cut(s) 151, 824, 968, 1400, 1666, 1688, 2389, 2590, 2900
BmiI GGNNCC 5 cut(s) 153, 2146, 2476, 3052, 3095
BmrFI CCNGG 5 cut(s) 89, 572, 763, 834, 978
BmsI GCATC 6 cut(s) 109, 343, 611, 1846, 2417, 2610
BoxI GACNNNNGTC 2 cut(s) 495, 579
BpiI GAAGAC 4 cut(s) 770, 1646, 1966, 2994
BpmI CTGGAG 4 cut(s) 228, 463, 2098, 2211
Bpu10I CCTNAGC 1 cut(s) 1924
Bpu1102I GCTNAGC 1 cut(s) 1152
Bpu14I TTCGAA 2 cut(s) 781, 2327
BpuEI CTTGAG 2 cut(s) 131, 263
BpuMI CCSGG 1 cut(s) 978
Bsa29I ATCGAT 1 cut(s) 1998
BsaBI GATNNNNATC 1 cut(s) 2403
BsaJI CCNNGG 7 cut(s) 761, 820, 2139, 2242, 2448, 2569, 3071
BsaWI WCCGGW 2 cut(s) 2669, 2684
Bse118I RCCGGY 2 cut(s) 230, 2684
Bse3DI GCAATG 6 cut(s) 85, 1146, 1935, 2162, 2286, 2893
Bse8I GATNNNNATC 1 cut(s) 2403
BseBI CCWGG 4 cut(s) 89, 572, 763, 834
BseCI ATCGAT 1 cut(s) 1998
BseDI CCNNGG 7 cut(s) 761, 820, 2139, 2242, 2448, 2569, 3071
BseGI GGATG 7 cut(s) 720, 815, 1114, 1142, 1430, 2579, 2790
BseJI GATNNNNATC 1 cut(s) 2403
BseMI GCAATG 6 cut(s) 85, 1146, 1935, 2162, 2286, 2893
BseRI GAGGAG 3 cut(s) 201, 1912, 2895
BseXI GCAGC 2 cut(s) 1846, 2288
BseYI CCCAGC 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 1845
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 234, 970
BshNI GGYRCC 1 cut(s) 3093
BshTI ACCGGT 1 cut(s) 2684
BshVI ATCGAT 1 cut(s) 1998
BsiHKAI GWGCWC 3 cut(s) 249, 2138, 2368
BsiSI CCGG 6 cut(s) 231, 978, 2624, 2670, 2685, 2874
BslFI GGGAC 3 cut(s) 646, 2730, 2844
BsmAI GTCTC 7 cut(s) 183, 503, 893, 937, 1924, 2337, 2645
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 3 cut(s) 646, 2730, 2844
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 2 cut(s) 234, 970
Bsp119I TTCGAA 2 cut(s) 781, 2327
Bsp1286I GDGCHC 3 cut(s) 249, 2138, 2368
Bsp1720I GCTNAGC 1 cut(s) 1152
Bsp19I CCATGG 2 cut(s) 2448, 3071
BspACI CCGC 3 cut(s) 180, 300, 2898
BspANI GGCC 2 cut(s) 234, 970
BspDI ATCGAT 1 cut(s) 1998
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 153, 2146, 2476, 3052, 3095
BspMI ACCTGC 1 cut(s) 2178
BspPI GGATC 5 cut(s) 3, 751, 1183, 1950, 2560
BspQI GCTCTTC 1 cut(s) 44
BspT104I TTCGAA 2 cut(s) 781, 2327
BspT107I GGYRCC 1 cut(s) 3093
BsrDI GCAATG 6 cut(s) 85, 1146, 1935, 2162, 2286, 2893
BsrFI RCCGGY 2 cut(s) 230, 2684
BssAI RCCGGY 2 cut(s) 230, 2684
BssECI CCNNGG 7 cut(s) 761, 820, 2139, 2242, 2448, 2569, 3071
BssT1I CCWWGG 6 cut(s) 820, 2139, 2242, 2448, 2569, 3071
Bst2UI CCWGG 4 cut(s) 89, 572, 763, 834
Bst4CI ACNGT 5 cut(s) 194, 1876, 2561, 2956, 3093
Bst6I CTCTTC 4 cut(s) 44, 1017, 1482, 2539
BstBI TTCGAA 2 cut(s) 781, 2327
BstC8I GCNNGC 4 cut(s) 1579, 2651, 2773, 2929
BstDSI CCRYGG 2 cut(s) 2448, 3071
BstF5I GGATG 7 cut(s) 720, 815, 1114, 1142, 1430, 2579, 2790
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 2448
BstHHI GCGC 2 cut(s) 2175, 2447
BstMAI GTCTC 7 cut(s) 183, 503, 893, 937, 1924, 2337, 2645
BstMWI GCNNNNNNNGC 3 cut(s) 1574, 2883, 2928
BstNI CCWGG 4 cut(s) 89, 572, 763, 834
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 2 cut(s) 495, 579
BstSCI CCNGG 5 cut(s) 87, 570, 761, 832, 976
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 1846, 2288
BstV2I GAAGAC 4 cut(s) 770, 1646, 1966, 2994
BstX2I RGATCY 4 cut(s) 8, 1188, 1942, 2565
BstXI CCANNNNNNTGG 3 cut(s) 1105, 1901, 2756
BstYI RGATCY 4 cut(s) 8, 1188, 1942, 2565
Bsu15I ATCGAT 1 cut(s) 1998
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 2 cut(s) 234, 970
BsuTUI ATCGAT 1 cut(s) 1998
BtgI CCRYGG 2 cut(s) 2448, 3071
BtsCI GGATG 7 cut(s) 720, 815, 1114, 1142, 1430, 2579, 2790
BtsI GCAGTG 1 cut(s) 2929
BtsIMutI CAGTG 5 cut(s) 538, 1304, 1416, 2053, 2929
BveI ACCTGC 1 cut(s) 2178
Cac8I GCNNGC 4 cut(s) 1579, 2651, 2773, 2929
CfoI GCGC 2 cut(s) 2175, 2447
Cfr10I RCCGGY 2 cut(s) 230, 2684
Cfr13I GGNCC 9 cut(s) 151, 824, 968, 1400, 1666, 1688, 2389, 2590, 2900
ClaI ATCGAT 1 cut(s) 1998
Csp6I GTAC 3 cut(s) 2415, 2512, 3094
CspAI ACCGGT 1 cut(s) 2684
CspCI CAANNNNNGTGG 2 cut(s) 2289, 2324
CviQI GTAC 3 cut(s) 2415, 2512, 3094
DriI GACNNNNNGTC 1 cut(s) 1300
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 4 cut(s) 44, 1017, 1482, 2539
Eam1105I GACNNNNNGTC 1 cut(s) 1300
EarI CTCTTC 4 cut(s) 44, 1017, 1482, 2539
Ecl136II GAGCTC 2 cut(s) 247, 2136
Eco130I CCWWGG 6 cut(s) 820, 2139, 2242, 2448, 2569, 3071
Eco24I GRGCYC 2 cut(s) 249, 2138
Eco32I GATATC 3 cut(s) 419, 546, 1208
Eco47I GGWCC 8 cut(s) 151, 824, 1400, 1666, 1688, 2389, 2590, 2900
Eco53kI GAGCTC 2 cut(s) 247, 2136
Eco57I CTGAAG 2 cut(s) 1640, 2840
EcoICRI GAGCTC 2 cut(s) 247, 2136
EcoO109I RGGNCCY 3 cut(s) 151, 824, 1688
EcoRI GAATTC 3 cut(s) 375, 1268, 2691
EcoRII CCWGG 4 cut(s) 87, 570, 761, 832
EcoRV GATATC 3 cut(s) 419, 546, 1208
EcoT14I CCWWGG 6 cut(s) 820, 2139, 2242, 2448, 2569, 3071
EcoT38I GRGCYC 2 cut(s) 249, 2138
ErhI CCWWGG 6 cut(s) 820, 2139, 2242, 2448, 2569, 3071
Esp3I CGTCTC 1 cut(s) 503
FaqI GGGAC 3 cut(s) 646, 2730, 2844
FbaI TGATCA 1 cut(s) 2866
FblI GTMKAC 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 1860, 2277
FokI GGATG 7 cut(s) 707, 822, 1121, 1129, 1417, 2586, 2797
FriOI GRGCYC 2 cut(s) 249, 2138
Fsp4HI GCNGC 2 cut(s) 1860, 2277
FspBI CTAG 8 cut(s) 467, 788, 947, 1670, 1722, 1916, 2531, 2723
FspI TGCGCA 1 cut(s) 2174
GlaI GCGC 2 cut(s) 2174, 2446
GluI GCNGC 2 cut(s) 1860, 2277
GsaI CCCAGC 1 cut(s) 441
GsuI CTGGAG 4 cut(s) 228, 463, 2098, 2211
HaeII RGCGCY 1 cut(s) 2448
HaeIII GGCC 2 cut(s) 234, 970
HapII CCGG 6 cut(s) 231, 978, 2624, 2670, 2685, 2874
HhaI GCGC 2 cut(s) 2175, 2447
Hin6I GCGC 2 cut(s) 2173, 2445
HinP1I GCGC 2 cut(s) 2173, 2445
HincII GTYRAC 3 cut(s) 286, 1872, 1879
HindII GTYRAC 3 cut(s) 286, 1872, 1879
HindIII AAGCTT 2 cut(s) 1579, 2266
HpaII CCGG 6 cut(s) 231, 978, 2624, 2670, 2685, 2874
HphI GGTGA 3 cut(s) 1124, 2258, 2947
Hpy166II GTNNAC 7 cut(s) 286, 485, 671, 1283, 1872, 1879, 3064
Hpy8I GTNNAC 7 cut(s) 286, 485, 671, 1283, 1872, 1879, 3064
Hpy99I CGWCG 1 cut(s) 1632
HpyAV CCTTC 6 cut(s) 324, 545, 754, 1535, 2330, 2502
HpyCH4III ACNGT 5 cut(s) 194, 1876, 2561, 2956, 3093
HpyCH4IV ACGT 2 cut(s) 496, 1087
HpyF10VI GCNNNNNNNGC 3 cut(s) 1574, 2883, 2928
HpySE526I ACGT 2 cut(s) 496, 1087
HspAI GCGC 2 cut(s) 2173, 2445
KpnI GGTACC 1 cut(s) 3097
Ksp22I TGATCA 1 cut(s) 2866
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 4 cut(s) 252, 639, 2141, 3075
Lsp1109I GCAGC 2 cut(s) 1846, 2288
LweI GCATC 6 cut(s) 109, 343, 611, 1846, 2417, 2610
MaeI CTAG 8 cut(s) 467, 788, 947, 1670, 1722, 1916, 2531, 2723
MaeII ACGT 2 cut(s) 496, 1087
MaeIII GTNAC 7 cut(s) 622, 876, 1124, 1302, 2196, 2533, 2578
MfeI CAATTG 1 cut(s) 1829
MflI RGATCY 4 cut(s) 8, 1188, 1942, 2565
MhlI GDGCHC 3 cut(s) 249, 2138, 2368
MlyI GAGTC 2 cut(s) 589, 1609
MmeI TCCRAC 5 cut(s) 543, 1086, 1770, 1796, 2048
MroXI GAANNNNTTC 2 cut(s) 1031, 2128
MslI CAYNNNNRTG 3 cut(s) 629, 2225, 2805
MspI CCGG 6 cut(s) 231, 978, 2624, 2670, 2685, 2874
MspR9I CCNGG 5 cut(s) 89, 572, 763, 834, 978
MunI CAATTG 1 cut(s) 1829
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 4 cut(s) 89, 572, 763, 834
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 3 cut(s) 1574, 2883, 2928
NciI CCSGG 1 cut(s) 978
NcoI CCATGG 2 cut(s) 2448, 3071
NlaIV GGNNCC 5 cut(s) 153, 2146, 2476, 3052, 3095
NmuCI GTSAC 5 cut(s) 622, 876, 1124, 1302, 2533
NsbI TGCGCA 1 cut(s) 2174
NspI RCATGY 1 cut(s) 634
NspV TTCGAA 2 cut(s) 781, 2327
PaqCI CACCTGC 1 cut(s) 2178
PciSI GCTCTTC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 493
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 2 cut(s) 1031, 2128
PflMI CCANNNNNTGG 2 cut(s) 2386, 2484
PinAI ACCGGT 1 cut(s) 2684
PkrI GCNGC 2 cut(s) 1861, 2278
PleI GAGTC 2 cut(s) 588, 1609
PpsI GAGTC 2 cut(s) 588, 1609
PpuMI RGGWCCY 3 cut(s) 151, 824, 1688
PshAI GACNNNNGTC 2 cut(s) 495, 579
PshBI ATTAAT 1 cut(s) 3132
PsiI TTATAA 2 cut(s) 510, 1530
Psp124BI GAGCTC 2 cut(s) 249, 2138
Psp5II RGGWCCY 3 cut(s) 151, 824, 1688
Psp6I CCWGG 4 cut(s) 87, 570, 761, 832
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 4 cut(s) 87, 570, 761, 832
PspN4I GGNNCC 5 cut(s) 153, 2146, 2476, 3052, 3095
PspPI GGNCC 9 cut(s) 151, 824, 968, 1400, 1666, 1688, 2389, 2590, 2900
PspPPI RGGWCCY 3 cut(s) 151, 824, 1688
PsuI RGATCY 4 cut(s) 8, 1188, 1942, 2565
RsaI GTAC 3 cut(s) 2416, 2513, 3095
RsaNI GTAC 3 cut(s) 2415, 2512, 3094
RseI CAYNNNNRTG 3 cut(s) 629, 2225, 2805
SacI GAGCTC 2 cut(s) 249, 2138
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 2 cut(s) 1860, 2277
Sau96I GGNCC 9 cut(s) 151, 824, 968, 1400, 1666, 1688, 2389, 2590, 2900
SchI GAGTC 2 cut(s) 589, 1609
ScrFI CCNGG 5 cut(s) 89, 572, 763, 834, 978
SduI GDGCHC 3 cut(s) 249, 2138, 2368
SfaNI GCATC 6 cut(s) 109, 343, 611, 1846, 2417, 2610
SfuI TTCGAA 2 cut(s) 781, 2327
SinI GGWCC 8 cut(s) 151, 824, 1400, 1666, 1688, 2389, 2590, 2900
SmiMI CAYNNNNRTG 3 cut(s) 629, 2225, 2805
SmlI CTYRAG 2 cut(s) 146, 242
SmoI CTYRAG 2 cut(s) 146, 242
SsiI CCGC 3 cut(s) 180, 300, 2898
SspI AATATT 1 cut(s) 682
SspMI CTAG 8 cut(s) 467, 788, 947, 1670, 1722, 1916, 2531, 2723
SstI GAGCTC 2 cut(s) 249, 2138
StyD4I CCNGG 5 cut(s) 87, 570, 761, 832, 976
StyI CCWWGG 6 cut(s) 820, 2139, 2242, 2448, 2569, 3071
TaaI ACNGT 5 cut(s) 194, 1876, 2561, 2956, 3093
TaiI ACGT 2 cut(s) 499, 1090
TscAI CASTG 5 cut(s) 545, 1311, 1416, 2053, 2929
TseFI GTSAC 5 cut(s) 622, 876, 1124, 1302, 2533
TseI GCWGC 2 cut(s) 1859, 2276
Tsp45I GTSAC 5 cut(s) 622, 876, 1124, 1302, 2533
TspGWI ACGGA 1 cut(s) 2848
TspRI CASTG 5 cut(s) 545, 1311, 1416, 2053, 2929
Van91I CCANNNNNTGG 2 cut(s) 2386, 2484
VpaK11BI GGWCC 8 cut(s) 151, 824, 1400, 1666, 1688, 2389, 2590, 2900
VspI ATTAAT 1 cut(s) 3132
XceI RCATGY 1 cut(s) 634
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XmiI GTMKAC 1 cut(s) 484
XmnI GAANNNNTTC 2 cut(s) 1031, 2128
XspI CTAG 8 cut(s) 467, 788, 947, 1670, 1722, 1916, 2531, 2723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.