MD15G1427000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000281
Physical Location & Seq
Forward (+)
52756200 .. 52760261
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1427000.v1.1.491

Sequence Viewer

Length: 2721 bp
ATGGCTAGAGTCATGAGACTTGTTCCACTACTCACCTTTCTTCTATCCATTGCGTCTTTTACACTTAGCTTATGCAATGGAAACCTGAATGTGCCTTGCCCAGAAAATGAGAGACAAACACTTCTTATGTTCAAGAAAGATCTCAATGATTCTTCAAATCTGCTTTCCTCTTGGGTTGCTGAAGGCGATTGTTGCACCTGGACCGGTGTTGCCTGCGACAATTTAACCGGTCATGTCCGTGAGCTCCACCTTGCTGCTTATTATGATGAAGTGGCTGGTGAGTACCATAGGCTGGGTGATGCAAGGTTCGACGGAACCGTTCCTCATCAGTTGGGAAATCTCTCGAGCCTGCGTTATCTGGACCTTTCTGGGAACTATGGTTCGAAGGTCGAGAATCTCAAATGGCTCTCTGGTCTTTCTCTGTTGAAACATCTTGGCATGAGTAATATAGATCTTACCAGGGCATCTCATTGGTTACAAGTAAACACACTCCCTTCTCTCCTGGTCGAGTTGCATTTGTCAAGCTGCGAACTTTATCACATACCAAGTGGTATTCCGAACTTGACAAGTCTTAAAGTTCTTGATCTACACGGTAACTATCTCAACTCTGCCATACCTACATGGTTGTACAGTTTGAACCATCTTGAGTCCCTTGATCTTTCTTCCAATGCTTTCCATGGTGAAATTTCGAGTTCCCTTGGAAACTTGACAGCCCTTGTTGATCTTGAATTAGGTTCTAATCAGCTTGAAGGGGAAATCCCAAACTCATTGGGAAATCTTTGTAAGTTGACTTCTTTTGATCTATCGTCAAACAATTTTCGAGGGAGGGTATCAGAAATCTTTGAAAGTTTGTCTAGCTGTAGTTTTGGTCAAATATATTCTTTACAGCTTTCAGATAATAATTTTTCAGGTCATTTATCTGATCAGTTGGGAATTTTTAAAAATTTACACCGCCTTGATCTTTCAAATAATTCAATATCAGGTCTCATTCCAGTGTCTTTAGGAAATCTATCACGCTTAGAGGAGTTGATCATTTCTAACAATTTATTCGAGGGCGTTGTCTCTGAAGCTCATTTTACTAATCTTACAAGATTGGATACCTTTTATGCAAATGAGAACTCCTTGACTCTTAAAACTAGCCCGGACTGGGTTCCTCCTTTTCAACTTTCTCTGTTGAGTTTAAGTTCTTGGCGCCTGGACCCATCACAGTTGCCTGCATGGCTTCAGAGTCAAAAACATTTGCTCGCTCTTAATATGTCCAGTACAGGAATTTCAGGTACCATTCCGGCTTGGTTGTGGAACATTTCTTCTGTTGAAAGCCAAGAAATGGGATATGCAAGTATCTTTGTGGATCTCTCGCGTAATCAATTGTCCGGGGAGGTTCCAAATATAGCTTCTACCCATTGGCAAAAACTGGATTTTTATTTGCAAACAGTTGACATTTTTTTAGGATCTAACCAATTCAACGGTTCATTGCCTCTTGTGTCTTCGGCAGTGTTTGTACTAGATCTTTCCAATTCATCATTTTCAGGAAGTCTCTCTCACTTTTTTTGTGATACGAGTGATGTACCTAAATACCTTCGAGTTCTTCTTCTTGACAACAATCACCTCACTGGAGAAATTCCTGATTGTTGGTTACACTGGCCAAACTTGACAGTAGTGAATTTAGAAGACAACAATTTGACAGGGAAAATTCCAAGCTCTATTGGAGACTTACTTCTCCTTCAGTCGTTGCACTTGCGCAATAACAACCTATCTGGAGATTTACCCGTGTCCCTCCAAAACTGCGAGCAGTTGTTACTTCTTGACCTTGGTGGAAACAAGTTTGTTGGAAGCATTCCAATATGGTTTGGCCAAAGCTTGGTGGTTCTTATTCTTCGTTCAAATAAGTTCCAAGGCACCATTCCTGATGAACTTTGTAGTCTCAGAACGCTCCAAATCTTGGACCTTGCACATAACAATCTCTCGGGAACGATACCTAGATGTTTCCAAAATTTGTCATCCATGGCCAATAAATTTACAAGCAAAGGAGCTTCCGGTACTGAATTCTCGGTTTATGAGTTTTATAGTTCTTCTTTTCAATTCTCCTACATAGAGAATGCAGTTTTTGTGGCCAAAGGCAGAGAAGTGAAATATAACACAGTGCTTCGTTTGGTAACTAGCTTGGACCTTTCAAGGAACATGTTATTTGGAGAAATCCCTGAAGAGTTGACCAACCTCACTAGTCTACAAACATTGAATTTATCTGATAATCTTCTGACCGGAAGAATCCCTTCCAAAATTGGTGATATGGGAGCGTTAGAGTCGCTTGATTTGTCTGTGAACCAACTTTCTGGCGAAATTTCTCCAAGCATCTCGAATTTAACATTTCTCAATCATCTAAATTTGTCCTATAACAATCTGATTGGGCAGATTCCAAAAAGCACTCAGCTTCAAAGCTTTGATTTGTCCAGTTATGTTGGCAATAAACTTTGCGGACCACCATTGGAAGAGCGTTGCATTAAAAATGAGGCCATGCCACCAGTAGGTGATGAGAAGCAGAGAGAAGGTCATTTTCTTGAAGACGGTGGTTTCTATATGAGTTTGGGGCTTGGTTTTGCATTCGGGTTTTGGATTGTTCTTGGTTCATTACTGTCTAATGTGCCATGGAGCAATGCATTTTCTCAGTTCCAAAATCGCATTGTGAAGAAGTTTTATGCTGCAATCGTTGAACGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

907

Amino Acids

100.29

Weight (kDa)

5.16

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 73 1.8e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 110 - 273 2.4e-11 Leucine-rich repeat region
LRR_8 PF13855 168 - 224 6.1e-09 Leucine rich repeat
LRR_8 PF13855 292 - 349 7.9e-06 Leucine rich repeat
LRR_8 PF13855 526 - 585 1.8e-06 Leucine rich repeat
LRR_8 PF13855 549 - 609 6.9e-06 Leucine rich repeat
LRR_8 PF13855 719 - 777 2.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1742
Acc65I GGTACC 1 cut(s) 1277
AccB1I GGYRCC 3 cut(s) 1191, 1277, 1898
AccB7I CCANNNNNTGG 4 cut(s) 292, 1327, 1861, 1942
AccI GTMKAC 1 cut(s) 2227
AccII CGCG 1 cut(s) 1360
AciI CCGC 2 cut(s) 952, 2475
AclI AACGTT 1 cut(s) 2713
AclWI GGATC 2 cut(s) 1359, 1459
AcoI YGGCCR 4 cut(s) 1643, 1852, 2007, 2112
AcuI CTGAAG 5 cut(s) 201, 1086, 1208, 1709, 2223
AcyI GRCGYC 1 cut(s) 1192
AfaI GTAC 7 cut(s) 284, 629, 1264, 1279, 1503, 1569, 2041
AfiI CCNNNNNNNGG 6 cut(s) 292, 1147, 1327, 1861, 1942, 2525
AflIII ACRYGT 1 cut(s) 2181
AgeI ACCGGT 2 cut(s) 203, 227
AhlI ACTAGT 1 cut(s) 2222
AjnI CCWGG 4 cut(s) 197, 458, 501, 1194
AjuI GAANNNNNNNTTGG 4 cut(s) 1378, 1410, 1887, 1919
AloI GAACNNNNNNTCC 2 cut(s) 364, 396
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 7 cut(s) 10, 106, 989, 1066, 1541, 1704, 1928
AlwI GGATC 2 cut(s) 1359, 1459
Ama87I CYCGRG 2 cut(s) 343, 1966
AoxI GGCC 5 cut(s) 1643, 1852, 2007, 2112, 2511
ApeKI GCWGC 3 cut(s) 254, 525, 2699
AsiGI ACCGGT 2 cut(s) 203, 227
Asp700I GAANNNNTTC 1 cut(s) 2272
Asp718I GGTACC 1 cut(s) 1277
AspLEI GCGC 2 cut(s) 1194, 1743
AspS9I GGNCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
AsuC2I CCSGG 2 cut(s) 1142, 1375
AsuHPI GGTGA 7 cut(s) 25, 290, 308, 692, 1598, 2297, 2540
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 2 cut(s) 343, 1966
AvaII GGWCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
BalI TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
BanI GGYRCC 3 cut(s) 1191, 1277, 1898
BanII GRGCYC 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 775, 807
BbsI GAAGAC 3 cut(s) 1479, 1677, 2568
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 241, 512, 2686
BccI CCATC 2 cut(s) 648, 1210
BciT130I CCWGG 4 cut(s) 199, 460, 503, 1196
BciVI GTATCC 1 cut(s) 1090
BclI TGATCA 2 cut(s) 922, 1029
BcnI CCSGG 2 cut(s) 1142, 1375
BcoDI GTCTC 7 cut(s) 10, 106, 989, 1066, 1541, 1704, 1928
BcuI ACTAGT 1 cut(s) 2222
BfaI CTAG 7 cut(s) 6, 855, 1137, 1505, 1980, 2160, 2223
BfmI CTRYAG 1 cut(s) 859
BfoI RGCGCY 1 cut(s) 1195
BfuI GTATCC 1 cut(s) 1090
BglI GCCNNNNNGGC 1 cut(s) 1219
BglII AGATCT 3 cut(s) 139, 451, 1507
BisI GCNGC 3 cut(s) 255, 526, 2700
BlsI GCNGC 3 cut(s) 256, 527, 2701
Bme1390I CCNGG 6 cut(s) 199, 460, 503, 1142, 1196, 1375
Bme18I GGWCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
BmeT110I CYCGRG 2 cut(s) 343, 1966
BmgT120I GGNCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
BmiI GGNNCC 7 cut(s) 316, 1152, 1193, 1200, 1279, 1383, 1900
BmrFI CCNGG 6 cut(s) 199, 460, 503, 1142, 1196, 1375
BmrI ACTGGG 1 cut(s) 1156
BmsI GCATC 3 cut(s) 289, 473, 2361
BmuI ACTGGG 1 cut(s) 1156
BpiI GAAGAC 3 cut(s) 1479, 1677, 2568
BpmI CTGGAG 2 cut(s) 1635, 1779
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 2 cut(s) 1142, 1375
BsaHI GRCGYC 1 cut(s) 1192
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 8 cut(s) 459, 676, 697, 1374, 1810, 1894, 2004, 2645
BsaWI WCCGGW 4 cut(s) 203, 227, 2036, 2261
Bsc4I CCNNNNNNNGG 6 cut(s) 292, 1147, 1327, 1861, 1942, 2525
Bse118I RCCGGY 2 cut(s) 203, 227
Bse1I ACTGG 8 cut(s) 992, 1151, 1260, 1419, 1618, 1646, 2451, 2522
Bse3DI GCAATG 4 cut(s) 48, 82, 1472, 2659
BseBI CCWGG 4 cut(s) 199, 460, 503, 1196
BseDI CCNNGG 8 cut(s) 459, 676, 697, 1374, 1810, 1894, 2004, 2645
BseGI GGATG 1 cut(s) 2000
BseLI CCNNNNNNNGG 6 cut(s) 292, 1147, 1327, 1861, 1942, 2525
BseMI GCAATG 4 cut(s) 48, 82, 1472, 2659
BseMII CTCAG 3 cut(s) 1939, 2441, 2678
BseNI ACTGG 8 cut(s) 992, 1151, 1260, 1419, 1618, 1646, 2451, 2522
BseRI GAGGAG 1 cut(s) 1037
BseXI GCAGC 3 cut(s) 241, 512, 2686
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 1360
BshFI GGCC 5 cut(s) 1645, 1854, 2009, 2114, 2513
BshNI GGYRCC 3 cut(s) 1191, 1277, 1898
BshTI ACCGGT 2 cut(s) 203, 227
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 2 cut(s) 343, 1966
BsiSI CCGG 7 cut(s) 204, 228, 1142, 1286, 1374, 2037, 2262
BslFI GGGAC 2 cut(s) 634, 1759
BslI CCNNNNNNNGG 6 cut(s) 292, 1147, 1327, 1861, 1942, 2525
BsmAI GTCTC 7 cut(s) 10, 106, 989, 1066, 1541, 1704, 1928
BsmFI GGGAC 2 cut(s) 634, 1759
BsmI GAATGC 3 cut(s) 1836, 2104, 2600
BsnI GGCC 5 cut(s) 1645, 1854, 2009, 2114, 2513
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 2 cut(s) 343, 1966
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 246
Bsp1407I TGTACA 1 cut(s) 627
Bsp19I CCATGG 3 cut(s) 676, 2004, 2645
BspACI CCGC 2 cut(s) 952, 2475
BspANI GGCC 5 cut(s) 1645, 1854, 2009, 2114, 2513
BspCNI CTCAG 3 cut(s) 1938, 2440, 2677
BspFNI CGCG 1 cut(s) 1360
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 7 cut(s) 316, 1152, 1193, 1200, 1279, 1383, 1900
BspPI GGATC 2 cut(s) 1359, 1459
BspQI GCTCTTC 1 cut(s) 2484
BspT104I TTCGAA 1 cut(s) 383
BspT107I GGYRCC 3 cut(s) 1191, 1277, 1898
BspTNI GGTCTC 1 cut(s) 989
BsrDI GCAATG 4 cut(s) 48, 82, 1472, 2659
BsrFI RCCGGY 2 cut(s) 203, 227
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 8 cut(s) 992, 1151, 1260, 1419, 1618, 1646, 2451, 2522
BssAI RCCGGY 2 cut(s) 203, 227
BssECI CCNNGG 8 cut(s) 459, 676, 697, 1374, 1810, 1894, 2004, 2645
BssNI GRCGYC 1 cut(s) 1192
BssT1I CCWWGG 6 cut(s) 676, 697, 1810, 1894, 2004, 2645
Bst2UI CCWGG 4 cut(s) 199, 460, 503, 1196
Bst6I CTCTTC 2 cut(s) 2199, 2484
BstACI GRCGYC 1 cut(s) 1192
BstAUI TGTACA 1 cut(s) 627
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 5 cut(s) 214, 350, 1215, 1245, 1790
BstDEI CTNAG 5 cut(s) 65, 1018, 1925, 2427, 2664
BstDSI CCRYGG 3 cut(s) 676, 2004, 2645
BstF5I GGATG 1 cut(s) 2000
BstFNI CGCG 1 cut(s) 1360
BstH2I RGCGCY 1 cut(s) 1195
BstHHI GCGC 2 cut(s) 1194, 1743
BstMAI GTCTC 7 cut(s) 10, 106, 989, 1066, 1541, 1704, 1928
BstMWI GCNNNNNNNGC 2 cut(s) 192, 1219
BstNI CCWGG 4 cut(s) 199, 460, 503, 1196
BstNSI RCATGY 1 cut(s) 2185
BstSCI CCNGG 6 cut(s) 197, 458, 501, 1140, 1194, 1373
BstSFI CTRYAG 1 cut(s) 859
BstUI CGCG 1 cut(s) 1360
BstV1I GCAGC 3 cut(s) 241, 512, 2686
BstV2I GAAGAC 3 cut(s) 1479, 1677, 2568
BstX2I RGATCY 5 cut(s) 139, 451, 1351, 1451, 1507
BstXI CCANNNNNNTGG 1 cut(s) 2333
BstYI RGATCY 5 cut(s) 139, 451, 1351, 1451, 1507
BsuI GTATCC 1 cut(s) 1090
BsuRI GGCC 5 cut(s) 1645, 1854, 2009, 2114, 2513
BtgI CCRYGG 3 cut(s) 676, 2004, 2645
BtsCI GGATG 1 cut(s) 2000
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 5 cut(s) 999, 1500, 1611, 1639, 2148
Cac8I GCNNGC 5 cut(s) 214, 350, 1215, 1245, 1790
CciI TCATGA 1 cut(s) 12
CfoI GCGC 2 cut(s) 1194, 1743
Cfr10I RCCGGY 2 cut(s) 203, 227
Cfr13I GGNCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
CseI GACGC 1 cut(s) 42
Csp6I GTAC 7 cut(s) 283, 628, 1263, 1278, 1502, 1568, 2040
CspAI ACCGGT 2 cut(s) 203, 227
CviQI GTAC 7 cut(s) 283, 628, 1263, 1278, 1502, 1568, 2040
DdeI CTNAG 5 cut(s) 65, 1018, 1925, 2427, 2664
DinI GGCGCC 1 cut(s) 1193
DraI TTTAAA 1 cut(s) 940
EaeI YGGCCR 4 cut(s) 1643, 1852, 2007, 2112
Eam1104I CTCTTC 2 cut(s) 2199, 2484
EarI CTCTTC 2 cut(s) 2199, 2484
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 6 cut(s) 676, 697, 1810, 1894, 2004, 2645
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 5 cut(s) 201, 1086, 1208, 1709, 2223
Eco88I CYCGRG 2 cut(s) 343, 1966
EcoICRI GAGCTC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 2045
EcoRII CCWGG 4 cut(s) 197, 458, 501, 1194
EcoT14I CCWWGG 6 cut(s) 676, 697, 1810, 1894, 2004, 2645
EcoT22I ATGCAT 1 cut(s) 2659
EcoT38I GRGCYC 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 1193
EheI GGCGCC 1 cut(s) 1193
ErhI CCWWGG 6 cut(s) 676, 697, 1810, 1894, 2004, 2645
FalI AAGNNNNNCTT 2 cut(s) 2257, 2289
FaqI GGGAC 2 cut(s) 634, 1759
FbaI TGATCA 2 cut(s) 922, 1029
FblI GTMKAC 1 cut(s) 2227
Fnu4HI GCNGC 3 cut(s) 255, 526, 2700
FokI GGATG 1 cut(s) 1987
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 255, 526, 2700
FspBI CTAG 7 cut(s) 6, 855, 1137, 1505, 1980, 2160, 2223
FspI TGCGCA 1 cut(s) 1742
GlaI GCGC 2 cut(s) 1193, 1742
GluI GCNGC 3 cut(s) 255, 526, 2700
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 2 cut(s) 1635, 1779
HaeII RGCGCY 1 cut(s) 1195
HaeIII GGCC 5 cut(s) 1645, 1854, 2009, 2114, 2513
HapII CCGG 7 cut(s) 204, 228, 1142, 1286, 1374, 2037, 2262
HgaI GACGC 1 cut(s) 42
HhaI GCGC 2 cut(s) 1194, 1743
Hin1I GRCGYC 1 cut(s) 1192
Hin6I GCGC 2 cut(s) 1192, 1741
HinP1I GCGC 2 cut(s) 1192, 1741
HincII GTYRAC 3 cut(s) 789, 1438, 2211
HindII GTYRAC 3 cut(s) 789, 1438, 2211
HindIII AAGCTT 2 cut(s) 1858, 2437
HinfI GANTC 9 cut(s) 9, 149, 394, 647, 1126, 1228, 2268, 2303, 2413
HpaII CCGG 7 cut(s) 204, 228, 1142, 1286, 1374, 2037, 2262
HphI GGTGA 7 cut(s) 25, 290, 308, 692, 1598, 2297, 2540
Hpy166II GTNNAC 6 cut(s) 484, 789, 1438, 2211, 2228, 2323
Hpy8I GTNNAC 6 cut(s) 484, 789, 1438, 2211, 2228, 2323
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 8 cut(s) 176, 379, 504, 743, 1589, 1733, 2283, 2540
HpyCH4IV ACGT 1 cut(s) 2713
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 1219
HpyF3I CTNAG 5 cut(s) 65, 1018, 1925, 2427, 2664
HpySE526I ACGT 1 cut(s) 2713
Hsp92I GRCGYC 1 cut(s) 1192
HspAI GCGC 2 cut(s) 1192, 1741
KasI GGCGCC 1 cut(s) 1191
KpnI GGTACC 1 cut(s) 1281
Ksp22I TGATCA 2 cut(s) 922, 1029
LguI GCTCTTC 1 cut(s) 2484
LmnI GCTCC 5 cut(s) 249, 1938, 2030, 2294, 2649
Lsp1109I GCAGC 3 cut(s) 241, 512, 2686
LweI GCATC 3 cut(s) 289, 473, 2361
MaeI CTAG 7 cut(s) 6, 855, 1137, 1505, 1980, 2160, 2223
MaeII ACGT 1 cut(s) 2713
MaeIII GTNAC 5 cut(s) 474, 593, 1635, 1797, 2155
MfeI CAATTG 1 cut(s) 1367
MflI RGATCY 5 cut(s) 139, 451, 1351, 1451, 1507
MhlI GDGCHC 1 cut(s) 246
MlsI TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
MluNI TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
Mly113I GGCGCC 1 cut(s) 1192
MlyI GAGTC 5 cut(s) 18, 656, 1120, 1237, 2312
MmeI TCCRAC 1 cut(s) 1810
Mox20I TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
Mph1103I ATGCAT 1 cut(s) 2659
MroXI GAANNNNTTC 1 cut(s) 2272
MscI TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
MseI TTAA 9 cut(s) 224, 573, 939, 1131, 1181, 1251, 2363, 2502, 2719
MslI CAYNNNNRTG 2 cut(s) 237, 992
Msp20I TGGCCA 4 cut(s) 1645, 1854, 2009, 2114
MspI CCGG 7 cut(s) 204, 228, 1142, 1286, 1374, 2037, 2262
MspR9I CCNGG 6 cut(s) 199, 460, 503, 1142, 1196, 1375
MunI CAATTG 1 cut(s) 1367
Mva1269I GAATGC 3 cut(s) 1836, 2104, 2600
MvaI CCWGG 4 cut(s) 199, 460, 503, 1196
MvnI CGCG 1 cut(s) 1360
MwoI GCNNNNNNNGC 2 cut(s) 192, 1219
NarI GGCGCC 1 cut(s) 1192
NciI CCSGG 2 cut(s) 1142, 1375
NcoI CCATGG 3 cut(s) 676, 2004, 2645
NlaIV GGNNCC 7 cut(s) 316, 1152, 1193, 1200, 1279, 1383, 1900
NsbI TGCGCA 1 cut(s) 1742
NsiI ATGCAT 1 cut(s) 2659
NspI RCATGY 1 cut(s) 2185
NspV TTCGAA 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 343
PagI TCATGA 1 cut(s) 12
PciI ACATGT 1 cut(s) 2181
PciSI GCTCTTC 1 cut(s) 2484
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 3 cut(s) 1836, 2104, 2600
PdmI GAANNNNTTC 1 cut(s) 2272
PfeI GAWTC 4 cut(s) 149, 394, 2268, 2413
PflMI CCANNNNNTGG 4 cut(s) 292, 1327, 1861, 1942
PinAI ACCGGT 2 cut(s) 203, 227
PkrI GCNGC 3 cut(s) 256, 527, 2701
PleI GAGTC 5 cut(s) 17, 655, 1120, 1236, 2311
PluTI GGCGCC 1 cut(s) 1195
PpsI GAGTC 5 cut(s) 17, 655, 1120, 1236, 2311
PscI ACATGT 1 cut(s) 2181
Psp124BI GAGCTC 1 cut(s) 246
Psp1406I AACGTT 1 cut(s) 2713
Psp6I CCWGG 4 cut(s) 197, 458, 501, 1194
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 4 cut(s) 197, 458, 501, 1194
PspN4I GGNNCC 7 cut(s) 316, 1152, 1193, 1200, 1279, 1383, 1900
PspPI GGNCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
PsuI RGATCY 5 cut(s) 139, 451, 1351, 1451, 1507
RsaI GTAC 7 cut(s) 284, 629, 1264, 1279, 1503, 1569, 2041
RsaNI GTAC 7 cut(s) 283, 628, 1263, 1278, 1502, 1568, 2040
RseI CAYNNNNRTG 2 cut(s) 237, 992
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 2484
SaqAI TTAA 9 cut(s) 224, 573, 939, 1131, 1181, 1251, 2363, 2502, 2719
SatI GCNGC 3 cut(s) 255, 526, 2700
Sau96I GGNCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
SchI GAGTC 5 cut(s) 18, 656, 1120, 1237, 2312
ScrFI CCNGG 6 cut(s) 199, 460, 503, 1142, 1196, 1375
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 3 cut(s) 289, 473, 2361
SfcI CTRYAG 1 cut(s) 859
SfoI GGCGCC 1 cut(s) 1193
Sfr274I CTCGAG 1 cut(s) 343
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
SlaI CTCGAG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 237, 992
SmlI CTYRAG 2 cut(s) 343, 644
SmoI CTYRAG 2 cut(s) 343, 644
SpeI ACTAGT 1 cut(s) 2222
SsiI CCGC 2 cut(s) 952, 2475
SspDI GGCGCC 1 cut(s) 1191
SspMI CTAG 7 cut(s) 6, 855, 1137, 1505, 1980, 2160, 2223
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 6 cut(s) 197, 458, 501, 1140, 1194, 1373
StyI CCWWGG 6 cut(s) 676, 697, 1810, 1894, 2004, 2645
TaiI ACGT 1 cut(s) 2716
TatI WGTACW 3 cut(s) 627, 1262, 1501
TfiI GAWTC 4 cut(s) 149, 394, 2268, 2413
Tru1I TTAA 9 cut(s) 224, 573, 939, 1131, 1181, 1251, 2363, 2502, 2719
Tru9I TTAA 9 cut(s) 224, 573, 939, 1131, 1181, 1251, 2363, 2502, 2719
TscAI CASTG 5 cut(s) 999, 1500, 1618, 1646, 2148
TseI GCWGC 3 cut(s) 254, 525, 2699
TspDTI ATGAA 5 cut(s) 282, 1461, 1509, 1926, 2616
TspGWI ACGGA 2 cut(s) 227, 327
TspRI CASTG 5 cut(s) 999, 1500, 1618, 1646, 2148
Van91I CCANNNNNTGG 4 cut(s) 292, 1327, 1861, 1942
VpaK11BI GGWCC 6 cut(s) 201, 361, 1198, 1945, 2167, 2477
XceI RCATGY 1 cut(s) 2185
XhoI CTCGAG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 2227
XmnI GAANNNNTTC 1 cut(s) 2272
XspI CTAG 7 cut(s) 6, 855, 1137, 1505, 1980, 2160, 2223
Zsp2I ATGCAT 1 cut(s) 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.