Rw0G015420

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00702
OG0000281
Physical Location & Seq
Reverse (-)
4352 .. 7680
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015420.1

Sequence Viewer

Length: 3201 bp
ATGAAGAGGACCATGAGAGCCTTCTTAATTCTCAGGTTGCTAACTATTGCAACCATTACTATTAGTATTGGTTCATGCAATGGAAACCTGGGTGTGCCTTGTAAAGAAAGCGAAAAACAAGCGCTTCTCATTTTCAAGCAAGATCTCATAGATCCTTCAAATCGGCTTTCATCATGGGTTGCTGAAGAACATTCCAACTGCTGCAATTGGGCTGGAGTTGTCTGTCATTACTCCACCGGCCACATCCGTGAGCTGCATCTCGATAACCCCGACTATTTGGTGGATTCCAACTCTTCCTTGGGCGGTAAGATAAATCCTTCCCTGCTCAATTTAACGCATCTCACCTACTTTAACCTAAGTAACAATAACTTTCAAGGGACAAAGATTCCTGGCTTCTTTGGTTCTCTAAATAGTTTAACTCATCTTGACCTCTCACAAGCAAGCTTCGGAGGAATGGTTCCTCATCAATTGGGAAATCTTTCCAGTCTAAGCCATCTCTTTCTTGGTGGCGACGGCCTGAAAGTTGAGAACATGCAATGGATGTCTGGTCTTTCTCAATTGGAACACCTGGACATGAGTGAAGTTGATCTTAGCAAAGCATCTGATCATTGGCTGCTAGTGACAAACATGCTCCCCTCTTTGGTAGAATTAAGCATGGGCGGTTGTCAACTTCATAGCATTCCACTTCTACCTATCATCAATTTTACTTCTCTTGCCATCCTTCATCTTTCTTGGAACAGATTTAATTCTTTGATGCCTGGGTGGATTTTTAGTCTTAGAAATATAGTTTCTCTTCATTTGGACGGTTGCGAGTTTCAAGGTCCAATTCCTAGCAGTCCACACAATATCACGTCTCTCAGGGAAATTGATCTCTTCCAGAATAATTTTACCCTTTCGATACCTAAGTGGTTGTTTAACCACAAAGATCTCACTGTTTTGCACCTGGGATTCAATGCACTTGAAGGGTCACTGCCAGATGGTATGGCGAATATGACTGGTCTTAAAATTCTTAATCTCGATGGGAACCGGTTCAATTCAACCATACCTGAGTGGTTGTATAGTTTTAACCATCTTGAGTCCTTGAGTCTATCTGTCAATAACTTTCAGGGTGAAATTTCAAGTTCTATTGGCAATTTGACATCCATTAACAACCTAGACTTTAATGGAAATCTGTTGGAAGCGAAGATCCCAAACTCATTGGGAAATCTTTGTAAGTTGATGGTTCTTGATCTTTCATGGAACCATATCACGGTTGGAAGCTTATCAGAAATATTTGAAACTTTGTCTGCGTGTGGTTCAAATAGAATTAAGTCATTGTCTTTCAGTTCCTGTAATATTTCTGGTCAGTTAACAGAGCAGGTAGGAACCTTTACAAACTTGAGCTCCCTTGATCTTTCTCATAATTCAATATCAGGTCCCATTCCAGTGTCCCTGGCTAATCTGTCACGCTTAGAAACTTTAGACATCTCTTGGAATCAGTTCAATGGAACTGTTCCAGAAAGCATTGGTCAACTCGAAATGCTAACGAATTTGGATATGTCCTATAATTCATTGGAAGGTGCAGTCTCTGAAGTTCATTTTTCTTGTCTTACTAGATTGTTAAACATCAATGGAAACGAAAATTCATTGATGCTCAATACTAGTCGAGATTGGATTCCTCCTTTTCAACTTCAGTACCTAAGATTAGATTCTTGGCATATAGGGCCTGAATTTCCTACGTGGATTCAGCGACAAGTCCAGTTAAAGGGTGTAAGCTTATCTAGTACAGGACTTTCGGGTACCATTCCAAATTGGGTTTGGCACTCATCTTTAGGTTCTCTTGATCTCTCTCACAATCAATTGCATGGAGAGATCCAAAGTTTAGTTGCTTCCCAGTTGTCAATAATTGACTTAAGTTCTAACCAGTTCAACGGTTCATTGCCTCTTGTTTCTTCCAATGTAAGCATACTGGATCTTTCCAATTCATCATTTTCTGGATCTGTCACCCATTTCTTTTGTGAAGGACCAAATAAGAAACTTGAATTTCTTTACCTTGGGAACAATCATCTCGTTGGGAGAATTCCTAATTGTTGGAGGAACTGGAAAAGCCTGAAATTTGTGAATTTGGAAAGCAACAAGTTGACTGGGAATATTCCAAGCTCCATGGGATACTTGCAAGGCCTCAGATCACTTCACTTGCGCAATAATCAGTTATCTGGGGAGTTACCGCTATCATTGCAGAAGTGTACTAAATTGCGGGTTGTTGACCTTGGTCTAAATAAGTTTGAGGGAAGCTTACCAACATGGTTGGGAAAAAGCCTTCCAAACTTGGGGGTTCTTAACCTTCGTTCAAATAAGCTTCAGGGTGACATTCCACATGAACTCTGCAATCTCACAAGTCTCCGAATCTTGGACATTGCCCATAACAATCTCTCCGGAACAATACCAAGATGTTTCAACAGTCTTGCCATGAATACCGTGTCAAAATCATTTTTCATAGCTTTAAGCAGCACACTTCCCCTAGATACTTTGGATTGGTGGGTTGAAGAATTTGCTTTTGGAGGAAAATATATGGATAATGCGGTATTGGAGACGAAAGGGAGAGAAGTGGAATACAGCAGCATCCTTGCATTAGCAACAGGCATTGACCTCTCCAATAACATTATATCTGGGGGGATTCCTGAGGAACTGACCAGCCTCTTTGGCTTGCACACATTAAACTTATCAAACAATCTTTTGACTGGAAGAATCCCTTCAAAGATTGGTAATTTGGGTCAGTTAGAATCTCTTGATTTGTCGAAGAACCGTCTTTTTGGTGAAATTCCTGCAAGCATGACCAAAATGACATTTCTGTGTCGCTTGAACTTGTCATACAACAATTTGACCGGACGGATTCCAGAAAGCACCCAGCTTCAGACCCTTGATCAATCCAGCTTTGTTGGCAATGAACTTTGTGGTCCTCCACTGATCAAGATTTGCAGTGCAAGCAAGGTGACACCACCAACAGTTGAGCAGCAGAGAGGAGATGATTTACTTGACGACAAGTGGTTCTACCTGAGCTTGGGATTGGGATTCGCGGTTGGTTTTTGGATTATACTTGGCTCCTTGCTCATTAACATGCCATGGAGCTGTGCTTTTTCGCAATTCCTCAATAGCATTGTGCTTAAAATTTATGCTTTAATTGTTAAGTATGTTTTAGCTAGTTACTTCTGTTCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

1066

Amino Acids

117.67

Weight (kDa)

5.92

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 6.7e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 107 - 197 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 285 - 344 3.9e-06 Leucine rich repeat
LRR_14 PF23598 323 - 532 1e-12 Leucine-rich repeat region
LRR_8 PF13855 437 - 494 4.6e-06 Leucine rich repeat
LRR_14 PF23598 438 - 578 5.5e-09 Leucine-rich repeat region
LRR_14 PF23598 695 - 803 1.7e-08 Leucine-rich repeat region
LRR_8 PF13855 744 - 804 1.7e-07 Leucine rich repeat
LRR_14 PF23598 888 - 971 2.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2180
Acc36I ACCTGC 1 cut(s) 1348
Acc65I GGTACC 1 cut(s) 1778
AccB1I GGYRCC 1 cut(s) 1778
AccII CGCG 1 cut(s) 3056
AccIII TCCGGA 1 cut(s) 2414
AciI CCGC 6 cut(s) 303, 660, 2207, 2236, 2561, 3056
AclWI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
AcoI YGGCCR 1 cut(s) 238
AcuI CTGAAG 5 cut(s) 204, 1590, 1655, 2324, 2876
AfaI GTAC 4 cut(s) 1676, 1765, 1780, 2227
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 6 cut(s) 640, 1249, 1744, 2309, 2389, 3040
AflII CTTAAG 1 cut(s) 1891
AgeI ACCGGT 1 cut(s) 1026
AhlI ACTAGT 1 cut(s) 1640
AjiI CACGTC 1 cut(s) 852
AjnI CCWGG 6 cut(s) 87, 388, 567, 757, 942, 1431
AjuI GAANNNNNNNTTGG 2 cut(s) 2520, 2552
AleI CACNNNNGTG 1 cut(s) 246
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
Alw21I GWGCWC 1 cut(s) 1385
Alw26I GTCTC 4 cut(s) 858, 1570, 2384, 2564
AlwI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
AlwNI CAGNNNCTG 2 cut(s) 1329, 1568
Aor13HI TCCGGA 1 cut(s) 2414
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 4 cut(s) 238, 514, 1703, 2158
ApeKI GCWGC 6 cut(s) 201, 253, 613, 2487, 2598, 2992
AsiGI ACCGGT 1 cut(s) 1026
Asp700I GAANNNNTTC 4 cut(s) 478, 1028, 1478, 2731
Asp718I GGTACC 1 cut(s) 1778
AspLEI GCGC 2 cut(s) 124, 2181
AspS9I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2936
AsuHPI GGTGA 6 cut(s) 334, 1121, 1975, 2357, 2807, 2983
AvaII GGWCC 5 cut(s) 9, 821, 1415, 2003, 2936
AxyI CCTNAGG 1 cut(s) 2661
BaeI ACNNNNGTAYC 4 cut(s) 890, 923, 1658, 1691
BanI GGYRCC 1 cut(s) 1778
BanII GRGCYC 1 cut(s) 1385
Bbv12I GWGCWC 1 cut(s) 1385
BbvI GCAGC 6 cut(s) 188, 240, 600, 2499, 2610, 3004
BccI CCATC 6 cut(s) 501, 725, 971, 1013, 1077, 1213
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 6 cut(s) 89, 390, 569, 759, 944, 1433
BciVI GTATCC 1 cut(s) 2141
BclI TGATCA 3 cut(s) 604, 2902, 2946
BcoDI GTCTC 4 cut(s) 858, 1570, 2384, 2564
BcuI ACTAGT 1 cut(s) 1640
BfaI CTAG 8 cut(s) 617, 831, 1154, 1593, 1641, 1761, 2501, 3180
BfoI RGCGCY 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 1891
BfuAI ACCTGC 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 2141
BglI GCCNNNNNGGC 1 cut(s) 2682
BglII AGATCT 2 cut(s) 142, 925
BisI GCNGC 6 cut(s) 202, 254, 614, 2488, 2599, 2993
BlsI GCNGC 6 cut(s) 203, 255, 615, 2489, 2600, 2994
Bme1390I CCNGG 6 cut(s) 89, 390, 569, 759, 944, 1433
Bme18I GGWCC 5 cut(s) 9, 821, 1415, 2003, 2936
BmgBI CACGTC 1 cut(s) 852
BmgT120I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2936
BmiI GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3082
BmrFI CCNGG 6 cut(s) 89, 390, 569, 759, 944, 1433
BmrI ACTGGG 2 cut(s) 1867, 2133
BmsI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2610
BmuI ACTGGG 2 cut(s) 1867, 2133
BoxI GACNNNNGTC 1 cut(s) 2250
BpmI CTGGAG 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 3035
BpuEI CTTGAG 3 cut(s) 1094, 1102, 1399
BsaAI YACGTR 1 cut(s) 1719
BsaJI CCNNGG 9 cut(s) 88, 297, 758, 943, 1431, 2032, 2142, 2248, 3101
BsaWI WCCGGW 3 cut(s) 1026, 2414, 2864
BsaXI ACNNNNNCTCC 6 cut(s) 441, 471, 1367, 1397, 2396, 2426
Bsc4I CCNNNNNNNGG 6 cut(s) 640, 1249, 1744, 2309, 2389, 3040
Bse118I RCCGGY 2 cut(s) 236, 1026
Bse21I CCTNAGG 1 cut(s) 2661
Bse3DI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2929
BseAI TCCGGA 1 cut(s) 2414
BseBI CCWGG 6 cut(s) 89, 390, 569, 759, 944, 1433
BseDI CCNNGG 9 cut(s) 88, 297, 758, 943, 1431, 2032, 2142, 2248, 3101
BseGI GGATG 5 cut(s) 243, 546, 717, 1139, 2601
BseLI CCNNNNNNNGG 6 cut(s) 640, 1249, 1744, 2309, 2389, 3040
BseMI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2929
BseMII CTCAG 6 cut(s) 46, 871, 1038, 2176, 2652, 3026
BseRI GAGGAG 1 cut(s) 3015
BseXI GCAGC 6 cut(s) 188, 240, 600, 2499, 2610, 3004
BseYI CCCAGC 1 cut(s) 2886
BsgI GTGCAG 1 cut(s) 1581
Bsh1236I CGCG 1 cut(s) 3056
BshFI GGCC 4 cut(s) 240, 516, 1705, 2160
BshNI GGYRCC 1 cut(s) 1778
BshTI ACCGGT 1 cut(s) 1026
BsiHKAI GWGCWC 1 cut(s) 1385
BsiSI CCGG 4 cut(s) 237, 1027, 2415, 2865
BslFI GGGAC 3 cut(s) 391, 1401, 1414
BslI CCNNNNNNNGG 6 cut(s) 640, 1249, 1744, 2309, 2389, 3040
BsmAI GTCTC 4 cut(s) 858, 1570, 2384, 2564
BsmBI CGTCTC 2 cut(s) 858, 2564
BsmFI GGGAC 3 cut(s) 391, 1401, 1414
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 4 cut(s) 240, 516, 1705, 2160
Bsp1286I GDGCHC 1 cut(s) 1385
Bsp13I TCCGGA 1 cut(s) 2414
Bsp19I CCATGG 2 cut(s) 2142, 3101
BspACI CCGC 6 cut(s) 303, 660, 2207, 2236, 2561, 3056
BspANI GGCC 4 cut(s) 240, 516, 1705, 2160
BspCNI CTCAG 6 cut(s) 45, 870, 1039, 2175, 2653, 3027
BspEI TCCGGA 1 cut(s) 2414
BspFNI CGCG 1 cut(s) 3056
BspLI GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3082
BspMI ACCTGC 1 cut(s) 1348
BspPI GGATC 5 cut(s) 146, 1180, 1846, 1959, 1984
BspT107I GGYRCC 1 cut(s) 1778
BspTI CTTAAG 1 cut(s) 1891
BsrDI GCAATG 6 cut(s) 85, 542, 1916, 2213, 2394, 2929
BsrFI RCCGGY 2 cut(s) 236, 1026
BssAI RCCGGY 2 cut(s) 236, 1026
BssECI CCNNGG 9 cut(s) 88, 297, 758, 943, 1431, 2032, 2142, 2248, 3101
BssT1I CCWWGG 5 cut(s) 297, 2032, 2142, 2248, 3101
Bst2UI CCWGG 6 cut(s) 89, 390, 569, 759, 944, 1433
Bst4CI ACNGT 9 cut(s) 806, 934, 1252, 1492, 1913, 2441, 2458, 2786, 2986
Bst6I CTCTTC 3 cut(s) 298, 798, 878
BstAFI CTTAAG 1 cut(s) 1891
BstBAI YACGTR 1 cut(s) 1719
BstC8I GCNNGC 4 cut(s) 442, 2687, 2809, 2965
BstDSI CCRYGG 2 cut(s) 2142, 3101
BstF5I GGATG 5 cut(s) 243, 546, 717, 1139, 2601
BstFNI CGCG 1 cut(s) 3056
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 2181
BstMAI GTCTC 4 cut(s) 858, 1570, 2384, 2564
BstMWI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2682, 2919, 2964
BstNI CCWGG 6 cut(s) 89, 390, 569, 759, 944, 1433
BstNSI RCATGY 3 cut(s) 535, 631, 3100
BstPAI GACNNNNGTC 1 cut(s) 2250
BstSCI CCNGG 6 cut(s) 87, 388, 567, 757, 942, 1431
BstUI CGCG 1 cut(s) 3056
BstV1I GCAGC 6 cut(s) 188, 240, 600, 2499, 2610, 3004
BstX2I RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
BstYI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
Bsu36I CCTNAGG 1 cut(s) 2661
BsuI GTATCC 1 cut(s) 2141
BsuRI GGCC 4 cut(s) 240, 516, 1705, 2160
BtgI CCRYGG 2 cut(s) 2142, 3101
BtrI CACGTC 1 cut(s) 852
BtsCI GGATG 5 cut(s) 243, 546, 717, 1139, 2601
BtsI GCAGTG 2 cut(s) 968, 2965
BtsIMutI CAGTG 5 cut(s) 930, 968, 1431, 2942, 2965
BveI ACCTGC 1 cut(s) 1348
Cac8I GCNNGC 4 cut(s) 442, 2687, 2809, 2965
CaiI CAGNNNCTG 2 cut(s) 1329, 1568
CfoI GCGC 2 cut(s) 124, 2181
Cfr10I RCCGGY 2 cut(s) 236, 1026
Cfr13I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2936
Csp6I GTAC 4 cut(s) 1675, 1764, 1779, 2226
CspAI ACCGGT 1 cut(s) 1026
CviQI GTAC 4 cut(s) 1675, 1764, 1779, 2226
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 3 cut(s) 298, 798, 878
EarI CTCTTC 3 cut(s) 298, 798, 878
Ecl136II GAGCTC 1 cut(s) 1383
Eco130I CCWWGG 5 cut(s) 297, 2032, 2142, 2248, 3101
Eco147I AGGCCT 1 cut(s) 2160
Eco24I GRGCYC 1 cut(s) 1385
Eco47I GGWCC 5 cut(s) 9, 821, 1415, 2003, 2936
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 1383
Eco57I CTGAAG 5 cut(s) 204, 1590, 1655, 2324, 2876
Eco81I CCTNAGG 1 cut(s) 2661
EcoICRI GAGCTC 1 cut(s) 1383
EcoO109I RGGNCCY 2 cut(s) 1415, 1703
EcoRI GAATTC 1 cut(s) 2058
EcoRII CCWGG 6 cut(s) 87, 388, 567, 757, 942, 1431
EcoT14I CCWWGG 5 cut(s) 297, 2032, 2142, 2248, 3101
EcoT38I GRGCYC 1 cut(s) 1385
ErhI CCWWGG 5 cut(s) 297, 2032, 2142, 2248, 3101
Esp3I CGTCTC 2 cut(s) 858, 2564
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 2716, 2748
FaqI GGGAC 3 cut(s) 391, 1401, 1414
FauI CCCGC 1 cut(s) 2229
FbaI TGATCA 3 cut(s) 604, 2902, 2946
Fnu4HI GCNGC 6 cut(s) 202, 254, 614, 2488, 2599, 2993
FokI GGATG 5 cut(s) 230, 553, 704, 1126, 2588
FriOI GRGCYC 1 cut(s) 1385
Fsp4HI GCNGC 6 cut(s) 202, 254, 614, 2488, 2599, 2993
FspBI CTAG 8 cut(s) 617, 831, 1154, 1593, 1641, 1761, 2501, 3180
FspI TGCGCA 1 cut(s) 2180
GlaI GCGC 2 cut(s) 123, 2180
GluI GCNGC 6 cut(s) 202, 254, 614, 2488, 2599, 2993
GsaI CCCAGC 1 cut(s) 2890
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 4 cut(s) 240, 516, 1705, 2160
HapII CCGG 4 cut(s) 237, 1027, 2415, 2865
HhaI GCGC 2 cut(s) 124, 2181
Hin6I GCGC 2 cut(s) 122, 2179
HinP1I GCGC 2 cut(s) 122, 2179
HincII GTYRAC 5 cut(s) 668, 1350, 1511, 2121, 2245
HindII GTYRAC 5 cut(s) 668, 1350, 1511, 2121, 2245
HindIII AAGCTT 5 cut(s) 442, 1258, 1753, 2272, 2336
HpaI GTTAAC 1 cut(s) 1350
HpaII CCGG 4 cut(s) 237, 1027, 2415, 2865
HphI GGTGA 6 cut(s) 334, 1121, 1975, 2357, 2807, 2983
Hpy166II GTNNAC 7 cut(s) 668, 839, 1350, 1511, 2121, 2226, 2245
Hpy188I TCNGA 7 cut(s) 449, 604, 1267, 1570, 2165, 2384, 2895
Hpy8I GTNNAC 7 cut(s) 668, 839, 1350, 1511, 2121, 2226, 2245
Hpy99I CGWCG 1 cut(s) 515
HpyCH4III ACNGT 9 cut(s) 806, 934, 1252, 1492, 1913, 2441, 2458, 2786, 2986
HpyCH4IV ACGT 2 cut(s) 851, 1718
HpyF10VI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2682, 2919, 2964
HpySE526I ACGT 2 cut(s) 851, 1718
HspAI GCGC 2 cut(s) 122, 2179
Kpn2I TCCGGA 1 cut(s) 2414
KpnI GGTACC 1 cut(s) 1782
Ksp22I TGATCA 3 cut(s) 604, 2902, 2946
KspAI GTTAAC 1 cut(s) 1350
LmnI GCTCC 5 cut(s) 636, 1388, 2144, 3086, 3105
Lsp1109I GCAGC 6 cut(s) 188, 240, 600, 2499, 2610, 3004
LweI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2610
MaeI CTAG 8 cut(s) 617, 831, 1154, 1593, 1641, 1761, 2501, 3180
MaeII ACGT 2 cut(s) 851, 1718
MaeIII GTNAC 9 cut(s) 359, 619, 966, 1443, 1981, 2202, 2345, 2971, 3182
MfeI CAATTG 4 cut(s) 205, 467, 557, 1838
MflI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
MhlI GDGCHC 1 cut(s) 1385
MlyI GAGTC 2 cut(s) 1085, 1093
MmeI TCCRAC 5 cut(s) 219, 312, 1155, 1234, 2051
MroI TCCGGA 1 cut(s) 2414
MroXI GAANNNNTTC 4 cut(s) 478, 1028, 1478, 2731
MslI CAYNNNNRTG 4 cut(s) 246, 1424, 2830, 3095
MspCI CTTAAG 1 cut(s) 1891
MspI CCGG 4 cut(s) 237, 1027, 2415, 2865
MspR9I CCNGG 6 cut(s) 89, 390, 569, 759, 944, 1433
MunI CAATTG 4 cut(s) 205, 467, 557, 1838
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 6 cut(s) 89, 390, 569, 759, 944, 1433
MvnI CGCG 1 cut(s) 3056
MwoI GCNNNNNNNGC 5 cut(s) 1702, 2215, 2682, 2919, 2964
NcoI CCATGG 2 cut(s) 2142, 3101
NlaIV GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3082
NmuCI GTSAC 6 cut(s) 619, 966, 1443, 1981, 2345, 2971
NsbI TGCGCA 1 cut(s) 2180
NspI RCATGY 3 cut(s) 535, 631, 3100
OliI CACNNNNGTG 1 cut(s) 246
PceI AGGCCT 1 cut(s) 2160
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 4 cut(s) 478, 1028, 1478, 2731
PinAI ACCGGT 1 cut(s) 1026
PkrI GCNGC 6 cut(s) 203, 255, 615, 2489, 2600, 2994
PleI GAGTC 2 cut(s) 1084, 1092
PpsI GAGTC 2 cut(s) 1084, 1092
Ppu21I YACGTR 1 cut(s) 1719
PpuMI RGGWCCY 1 cut(s) 1415
PshAI GACNNNNGTC 1 cut(s) 2250
Psp124BI GAGCTC 1 cut(s) 1385
Psp5II RGGWCCY 1 cut(s) 1415
Psp6I CCWGG 6 cut(s) 87, 388, 567, 757, 942, 1431
PspFI CCCAGC 1 cut(s) 2886
PspGI CCWGG 6 cut(s) 87, 388, 567, 757, 942, 1431
PspN4I GGNNCC 7 cut(s) 459, 1025, 1241, 1366, 1417, 1780, 3082
PspPI GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2936
PspPPI RGGWCCY 1 cut(s) 1415
PsrI GAACNNNNNNTAC 2 cut(s) 2834, 2866
PstNI CAGNNNCTG 2 cut(s) 1329, 1568
PsuI RGATCY 7 cut(s) 142, 151, 925, 1185, 1851, 1951, 1976
RsaI GTAC 4 cut(s) 1676, 1765, 1780, 2227
RsaNI GTAC 4 cut(s) 1675, 1764, 1779, 2226
RseI CAYNNNNRTG 4 cut(s) 246, 1424, 2830, 3095
SacI GAGCTC 1 cut(s) 1385
SatI GCNGC 6 cut(s) 202, 254, 614, 2488, 2599, 2993
Sau96I GGNCC 6 cut(s) 9, 821, 1415, 1703, 2003, 2936
SchI GAGTC 2 cut(s) 1085, 1093
ScrFI CCNGG 6 cut(s) 89, 390, 569, 759, 944, 1433
SduI GDGCHC 1 cut(s) 1385
SfaNI GCATC 6 cut(s) 265, 346, 608, 744, 1620, 2610
SinI GGWCC 5 cut(s) 9, 821, 1415, 2003, 2936
SmiMI CAYNNNNRTG 4 cut(s) 246, 1424, 2830, 3095
SmlI CTYRAG 4 cut(s) 1073, 1081, 1378, 1891
SmoI CTYRAG 4 cut(s) 1073, 1081, 1378, 1891
SpeI ACTAGT 1 cut(s) 1640
SseBI AGGCCT 1 cut(s) 2160
SsiI CCGC 6 cut(s) 303, 660, 2207, 2236, 2561, 3056
SspI AATATT 3 cut(s) 1272, 1336, 2131
SspMI CTAG 8 cut(s) 617, 831, 1154, 1593, 1641, 1761, 2501, 3180
SstI GAGCTC 1 cut(s) 1385
StuI AGGCCT 1 cut(s) 2160
StyD4I CCNGG 6 cut(s) 87, 388, 567, 757, 942, 1431
StyI CCWWGG 5 cut(s) 297, 2032, 2142, 2248, 3101
TaaI ACNGT 9 cut(s) 806, 934, 1252, 1492, 1913, 2441, 2458, 2786, 2986
TaiI ACGT 2 cut(s) 854, 1721
TaqI TCGA 6 cut(s) 261, 896, 1017, 1515, 1645, 2777
TatI WGTACW 2 cut(s) 1763, 2225
TscAI CASTG 5 cut(s) 937, 975, 1431, 2949, 2965
TseFI GTSAC 6 cut(s) 619, 966, 1443, 1981, 2345, 2971
TseI GCWGC 6 cut(s) 201, 253, 613, 2487, 2598, 2992
Tsp45I GTSAC 6 cut(s) 619, 966, 1443, 1981, 2345, 2971
TspGWI ACGGA 2 cut(s) 236, 2884
TspRI CASTG 5 cut(s) 937, 975, 1431, 2949, 2965
Vha464I CTTAAG 1 cut(s) 1891
VpaK11BI GGWCC 5 cut(s) 9, 821, 1415, 2003, 2936
XceI RCATGY 3 cut(s) 535, 631, 3100
XcmI CCANNNNNNNNNTGG 4 cut(s) 295, 500, 1250, 1794
XmnI GAANNNNTTC 4 cut(s) 478, 1028, 1478, 2731
XspI CTAG 8 cut(s) 617, 831, 1154, 1593, 1641, 1761, 2501, 3180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.