MD01G1172800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000281
Physical Location & Seq
Reverse (-)
27476194 .. 27479142
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1172800.v1.1.491

Sequence Viewer

Length: 2949 bp
ATGGAGAGAAGCATGAGAGTTGTTTTACTACTAATCAGGGTTCTAGCTATTGCAACCATTACTTTCGGTATTGGTTTATGCAATGGAATTCCCGGTTGGCCTCCGCTTTGCAAAGAAAGCGAAAGACAAGCACTTATGATGTTCAAGCAAGATCTCGAGGACCCTGCCAATCGGCTTTCGTCATGGGTTGCAGAAGAAGGTTCAGACTGTTGCAGTTGGACAGGAGTTGTCTGTGATCACATAACCGGCCACATCCATGAGCTGCACCTTAATAGTTCCTACTCTGATGGGGTTTTCTACGCTTCTTTCGGTGGTAAGATAAATCCTTCTTTGCTCAGTTTAAAGCATCTCAACTTCTTGGACCTAAGCAACAATGATTTCAGTACAACACGAATTCCTAGTTTCTTTGGTTCTATGACAAGTTTAACACACCTTAATCTTGGAAACTCAGCGTTTGGTGGAGTAATTCCTCATAAACTGGGAAATCTCTCCAGTCTACGCTATCTCAATCTCAGTACTTTCCATTCCAATCTGAAGGTAGAGAACCTTCAGTGGATTTCTGGTCTTTCTTTGCTAAAACACTTGGACTTGGGTTATGTAAATCTTAGCAAAGCATCTGACTGGTTGCAAGTTACAAACACGCTCCCTTCTTTAGTAGAGTTAATTATGTCCGATTGTGAACTTGATCAAATTCCCCCTCTACCCACCACAAATTTTACTTCCCTGGTCGTCCTTGATCTTTCTGGAAACAGTTTTAATTCTTTGATGCCGAGGTGGGTTTTCAGTATTAAAAATCTAGTTTCTCTTCATCTCAGTTTTTGTGGTTTCCACGGTCCAATTCCGGGCAGTTCACAAAATATCACATCTTTGAGGGAAATCGATTTGTCACACAATTCTATTAGTCTTGATCCGATGCCCAAATGGTGGTTTAACCAAAAATTCCTTGAATTGAGTCTAGAAGCCAATCAACTTACAGGACAACTTCCAAGCAGTATTCAGAATATGACTAGTCTTACATCTCTTAATCTTGGGGGGAACGAATTCAATTCTACCATACCTGAATGGTTGTATAGCTTGAACAATCTCGAGTCCTTAATTCTTTATGGCAATGCCTTACGTGGTGAAATATCGAGTTCCATTGGAAACCTTAAGAGTTTAAGGCACTTTGATCTTTCAGGTAATTCAATATCAGGTCCCATTCCAATGTCCCTAGGAAATCTGTCAAGCTTAGTAGAACTAGACATATCTGGAAATCAGTTTAATGGAACTTTAATAGAAGTTATTGGTGAACTCAAAATGCTAACAGATTTGGATATATCTTATAATTCGTTAGAAGGTGTAGTGTCGGAAGTTATTTTTAGCAACCTTAAAAAGTTGAAGCGTTTCAGTGCACAAGATAATTCATTGACTTTGAAAACTAGTCGAGGTTGGCTTCCTCCCTTTCAACTTGAAAGTTTGCAATTGGATTCATGGCGCCTGGGACCTGAATGGCCAATGTGGCTTCAGAAACAAACGCAATTAAAAAAACTAAGCTTGTCTGGTACAAGAATTTCAAGTACTATTCCAACTTGGTTTTGGAACTTAACATTCCAATTAGATTATCTGAATCTCTCTCACAATCAATTGTACGGGGAGATTCAAAATATAGTTGCTGCTCCTGTTTCAGTAGTTGATCTTGGTTCCAACCAATTCACTGGTGCATTGCCTATTGTTCCCACCTCATTAGATCGGCTTGATCTTTCCAATTCATCATTTTCTGGATCTGTTTTCCACTTCTTCTGTGGTAGGAGGGATGAACCTTACCAACTTTCTATTCTTCATCTCGAGAACAATCATCTCACTGGAAAAGTACCCGACTGTTGGATGAATTGGACATCATTGGGATTCCTACATTTAGAAAACAACAACCTAACTGGGAATGTCCCAATGTCCATGGGATACTTGCTAAATCTACAATCGCTGCACTTGCGCAATAATCACCTGTACGGAGAATTGCCACATTCCCTAGAAAACTGTACCATGTTGTCAGTTGTTGACCTTAGTGGAAATGGGTTTGTCGGAAGCATACCAATATGGATGGGTAAAAGCCTTTCAGAGTTGCAGGTTCTTAACCTTCGTTCGAATGAGTTTGAGGGAGACATTCCTTCTGAAATTTGTTATTTGAAAAGTCTCCAGATATTGGACCTTGCACGTAATAAACTCTCTGGAACGATACCGAGATGCTTCCACAATTTGAGCGCCATGGCTGATTTGTCAGAATCCGTTTGGCCAACAATGTTTTCGCAAAGTGATGGTATTATGGAATTTACAAATGTAGAGAATGCAGTCTTGGTAACGAAAGGGAGAGAAATGGAATATAACAAGATTCTGGAATTCGTAAAATTCATGGACCTTTCATGCAACTTTATGTATGGAGAGATCCCTGAAGAACTTACCGACCTCCTCGCATTGCAGTCACTCAATTTATCGAATAACCGCTTCACCGGAAGAATTCCTTCAAAGATTGGTAATATGGCACAGTTAGAATCTCTCGATTTTTCCATGAACCAACTTGATGGTGAAATTCCTCAAAGCATGACGAATTTGACATTTCTGAGTCACTTAAACTTGTCCTACAACAATTTGACGGGACGAATTCCGAAAAGCACTCAGCTGCAGCTCCTTGATCAGTCTAGCTTCGTCGGCAACGAACTATGCGGAGCTCCACTCCACAAGAATTGCAGCCCAAATGGGGTGATACCGCCACCAACAGTTGAGCAAGACGGAGGAGGAGGATACAGTTTACTCGAAGACAAGTGGTTCTACATGAGCTTGGGAGTTGGATTCTTCACGGGGTTTTGGATTGTGCTTGGTTCTTTGCTAGTAAACATGCCATGGAGCATTCTTCTTTCACAGTTGCTGAATAGGATAGTGCTTAAAATGTATCATGTAATTGTTGAATATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

983

Amino Acids

109.48

Weight (kDa)

5.33

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 79 1.4e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 113 - 271 7.5e-09 Leucine-rich repeat region
LRR_8 PF13855 116 - 172 5.8e-06 Leucine rich repeat
LRR_8 PF13855 216 - 274 7.1e-06 Leucine rich repeat
LRR_8 PF13855 316 - 372 2e-06 Leucine rich repeat
LRR_14 PF23598 322 - 512 4.1e-13 Leucine-rich repeat region
LRR_14 PF23598 667 - 758 6.3e-07 Leucine-rich repeat region
LRR_8 PF13855 675 - 734 3.1e-06 Leucine rich repeat
LRR_8 PF13855 699 - 751 1e-05 Leucine rich repeat
LRR_14 PF23598 792 - 890 2.4e-07 Leucine-rich repeat region
LRR_8 PF13855 817 - 875 2.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1323
Acc16I TGCGCA 1 cut(s) 1970
Acc36I ACCTGC 1 cut(s) 2092
AccB1I GGYRCC 1 cut(s) 1473
AccB7I CCANNNNNTGG 2 cut(s) 924, 2179
AccI GTMKAC 1 cut(s) 496
AciI CCGC 4 cut(s) 104, 2476, 2697, 2741
AclWI GGATC 3 cut(s) 902, 1768, 2413
AcoI YGGCCR 3 cut(s) 247, 1490, 2267
AcuI CTGAAG 4 cut(s) 533, 554, 1487, 2445
AcyI GRCGYC 1 cut(s) 1474
AfaI GTAC 8 cut(s) 385, 517, 1543, 1558, 1628, 1851, 1985, 2017
AfiI CCNNNNNNNGG 5 cut(s) 842, 924, 1860, 2179, 2731
AflII CTTAAG 1 cut(s) 1148
AhlI ACTAGT 2 cut(s) 1007, 1418
AjnI CCWGG 2 cut(s) 723, 1476
AjuI GAANNNNNNNTTGG 8 cut(s) 79, 111, 1797, 1829, 2263, 2295, 2312, 2344
Alw21I GWGCWC 2 cut(s) 1393, 2704
Alw26I GTCTC 2 cut(s) 2130, 2174
Alw44I GTGCAC 1 cut(s) 1389
AlwI GGATC 3 cut(s) 902, 1768, 2413
AlwNI CAGNNNCTG 1 cut(s) 2899
Ama87I CYCGRG 3 cut(s) 155, 1085, 1823
AoxI GGCC 4 cut(s) 98, 247, 1490, 2267
ApaLI GTGCAC 1 cut(s) 1389
ApeKI GCWGC 6 cut(s) 262, 1652, 1960, 2653, 2656, 2721
Asp700I GAANNNNTTC 3 cut(s) 1040, 1382, 2494
AspA2I CCTAGG 1 cut(s) 1210
AspLEI GCGC 3 cut(s) 1476, 1971, 2240
AspS9I GGNCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
AsuC2I CCSGG 2 cut(s) 93, 843
AsuHPI GGTGA 6 cut(s) 1133, 1298, 1970, 2473, 2570, 2746
AsuII TTCGAA 1 cut(s) 2120
AvaI CYCGRG 3 cut(s) 155, 1085, 1823
AvaII GGWCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
AvrII CCTAGG 1 cut(s) 1210
BaeGI GKGCMC 1 cut(s) 1393
BalI TGGCCA 2 cut(s) 1492, 2269
BanI GGYRCC 1 cut(s) 1473
BanII GRGCYC 1 cut(s) 2704
BarI GAAGNNNNNNTAC 2 cut(s) 531, 563
BbsI GAAGAC 1 cut(s) 2796
Bbv12I GWGCWC 2 cut(s) 1393, 2704
BbvI GCAGC 6 cut(s) 249, 1639, 1947, 2640, 2668, 2733
BccI CCATC 4 cut(s) 281, 2071, 2285, 2549
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 2 cut(s) 725, 1478
BciVI GTATCC 2 cut(s) 1931, 2768
BclI TGATCA 3 cut(s) 235, 685, 2665
BcnI CCSGG 2 cut(s) 93, 843
BcoDI GTCTC 2 cut(s) 2130, 2174
BcuI ACTAGT 2 cut(s) 1007, 1418
BfmI CTRYAG 1 cut(s) 2654
BfoI RGCGCY 2 cut(s) 1477, 2241
BfrI CTTAAG 1 cut(s) 1148
BfuAI ACCTGC 1 cut(s) 2092
BfuI GTATCC 2 cut(s) 1931, 2768
BglI GCCNNNNNGGC 1 cut(s) 1498
BglII AGATCT 1 cut(s) 151
BisI GCNGC 6 cut(s) 263, 1653, 1961, 2654, 2657, 2722
BlnI CCTAGG 1 cut(s) 1210
BlsI GCNGC 6 cut(s) 264, 1654, 1962, 2655, 2658, 2723
BmcAI AGTACT 2 cut(s) 517, 1558
Bme1390I CCNGG 4 cut(s) 93, 725, 843, 1478
Bme18I GGWCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
BmeT110I CYCGRG 3 cut(s) 155, 1085, 1823
BmgT120I GGNCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
BmiI GGNNCC 5 cut(s) 162, 1195, 1475, 1482, 1681
BmrFI CCNGG 4 cut(s) 93, 725, 843, 1478
BmrI ACTGGG 2 cut(s) 488, 1923
BmsI GCATC 5 cut(s) 355, 623, 756, 903, 2210
BmuI ACTGGG 2 cut(s) 488, 1923
BpiI GAAGAC 1 cut(s) 2796
BplI GAGNNNNNCTC 2 cut(s) 2691, 2723
BpmI CTGGAG 2 cut(s) 475, 2156
Bpu10I CCTNAGC 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 2120
BpuMI CCSGG 2 cut(s) 93, 843
Bsa29I ATCGAT 1 cut(s) 879
BsaAI YACGTR 2 cut(s) 1118, 2192
BsaHI GRCGYC 1 cut(s) 1474
BsaJI CCNNGG 8 cut(s) 723, 770, 829, 1210, 1477, 1932, 2241, 2873
BsaWI WCCGGW 1 cut(s) 2483
Bsc4I CCNNNNNNNGG 5 cut(s) 842, 924, 1860, 2179, 2731
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 6 cut(s) 483, 492, 626, 1699, 1846, 1918
Bse3DI GCAATG 4 cut(s) 88, 1114, 1700, 2447
BseBI CCWGG 2 cut(s) 725, 1478
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 8 cut(s) 723, 770, 829, 1210, 1477, 1932, 2241, 2873
BseGI GGATG 4 cut(s) 252, 1798, 1869, 2082
BseLI CCNNNNNNNGG 5 cut(s) 842, 924, 1860, 2179, 2731
BseMI GCAATG 4 cut(s) 88, 1114, 1700, 2447
BseMII CTCAG 6 cut(s) 349, 462, 526, 826, 2585, 2663
BseNI ACTGG 6 cut(s) 483, 492, 626, 1699, 1846, 1918
BseRI GAGGAG 3 cut(s) 2432, 2781, 2784
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 6 cut(s) 249, 1639, 1947, 2640, 2668, 2733
BsgI GTGCAG 2 cut(s) 248, 1946
BshFI GGCC 4 cut(s) 100, 249, 1492, 2269
BshNI GGYRCC 1 cut(s) 1473
BshVI ATCGAT 1 cut(s) 879
BsiHKAI GWGCWC 2 cut(s) 1393, 2704
BsiHKCI CYCGRG 3 cut(s) 155, 1085, 1823
BsiSI CCGG 4 cut(s) 93, 246, 842, 2484
BslFI GGGAC 5 cut(s) 1179, 1192, 1494, 1907, 2643
BslI CCNNNNNNNGG 5 cut(s) 842, 924, 1860, 2179, 2731
BsmAI GTCTC 2 cut(s) 2130, 2174
BsmFI GGGAC 5 cut(s) 1179, 1192, 1494, 1907, 2643
BsmI GAATGC 2 cut(s) 2326, 2880
BsnI GGCC 4 cut(s) 100, 249, 1492, 2269
BsoBI CYCGRG 3 cut(s) 155, 1085, 1823
Bsp119I TTCGAA 1 cut(s) 2120
Bsp1286I GDGCHC 2 cut(s) 1393, 2704
Bsp19I CCATGG 3 cut(s) 1932, 2241, 2873
BspACI CCGC 4 cut(s) 104, 2476, 2697, 2741
BspANI GGCC 4 cut(s) 100, 249, 1492, 2269
BspCNI CTCAG 6 cut(s) 348, 461, 525, 825, 2586, 2662
BspDI ATCGAT 1 cut(s) 879
BspLI GGNNCC 5 cut(s) 162, 1195, 1475, 1482, 1681
BspMAI CTGCAG 1 cut(s) 2658
BspMI ACCTGC 1 cut(s) 2092
BspPI GGATC 3 cut(s) 902, 1768, 2413
BspT104I TTCGAA 1 cut(s) 2120
BspT107I GGYRCC 1 cut(s) 1473
BspTI CTTAAG 1 cut(s) 1148
BsrDI GCAATG 4 cut(s) 88, 1114, 1700, 2447
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 6 cut(s) 483, 492, 626, 1699, 1846, 1918
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 8 cut(s) 723, 770, 829, 1210, 1477, 1932, 2241, 2873
BssNI GRCGYC 1 cut(s) 1474
BssT1I CCWWGG 4 cut(s) 1210, 1932, 2241, 2873
Bst2UI CCWGG 2 cut(s) 725, 1478
Bst4CI ACNGT 9 cut(s) 209, 752, 833, 1859, 2015, 2520, 2752, 2780, 2895
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 1 cut(s) 1474
BstAFI CTTAAG 1 cut(s) 1148
BstBAI YACGTR 2 cut(s) 1118, 2192
BstBI TTCGAA 1 cut(s) 2120
BstDSI CCRYGG 4 cut(s) 829, 1932, 2241, 2873
BstF5I GGATG 4 cut(s) 252, 1798, 1869, 2082
BstH2I RGCGCY 2 cut(s) 1477, 2241
BstHHI GCGC 3 cut(s) 1476, 1971, 2240
BstMAI GTCTC 2 cut(s) 2130, 2174
BstMWI GCNNNNNNNGC 4 cut(s) 117, 1498, 1966, 2682
BstNI CCWGG 2 cut(s) 725, 1478
BstNSI RCATGY 1 cut(s) 2872
BstSCI CCNGG 4 cut(s) 91, 723, 841, 1476
BstSFI CTRYAG 1 cut(s) 2654
BstSLI GKGCMC 1 cut(s) 1393
BstV1I GCAGC 6 cut(s) 249, 1639, 1947, 2640, 2668, 2733
BstV2I GAAGAC 1 cut(s) 2796
BstX2I RGATCY 3 cut(s) 151, 1760, 2418
BstXI CCANNNNNNTGG 2 cut(s) 1694, 2555
BstYI RGATCY 3 cut(s) 151, 1760, 2418
Bsu15I ATCGAT 1 cut(s) 879
BsuI GTATCC 2 cut(s) 1931, 2768
BsuRI GGCC 4 cut(s) 100, 249, 1492, 2269
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 4 cut(s) 829, 1932, 2241, 2873
BtsCI GGATG 4 cut(s) 252, 1798, 1869, 2082
BtsIMutI CAGTG 4 cut(s) 557, 1393, 1692, 1839
BveI ACCTGC 1 cut(s) 2092
CaiI CAGNNNCTG 1 cut(s) 2899
CfoI GCGC 3 cut(s) 1476, 1971, 2240
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
ClaI ATCGAT 1 cut(s) 879
Csp6I GTAC 8 cut(s) 384, 516, 1542, 1557, 1627, 1850, 1984, 2016
CspCI CAANNNNNGTGG 2 cut(s) 2699, 2734
CviQI GTAC 8 cut(s) 384, 516, 1542, 1557, 1627, 1850, 1984, 2016
DinI GGCGCC 1 cut(s) 1475
DraI TTTAAA 1 cut(s) 342
EaeI YGGCCR 3 cut(s) 247, 1490, 2267
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
Ecl136II GAGCTC 1 cut(s) 2702
Eco130I CCWWGG 4 cut(s) 1210, 1932, 2241, 2873
Eco24I GRGCYC 1 cut(s) 2704
Eco47I GGWCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
Eco53kI GAGCTC 1 cut(s) 2702
Eco57I CTGAAG 4 cut(s) 533, 554, 1487, 2445
Eco88I CYCGRG 3 cut(s) 155, 1085, 1823
EcoICRI GAGCTC 1 cut(s) 2702
EcoO109I RGGNCCY 3 cut(s) 160, 1193, 1481
EcoRI GAATTC 6 cut(s) 87, 393, 1040, 2372, 2490, 2634
EcoRII CCWGG 2 cut(s) 723, 1476
EcoT14I CCWWGG 4 cut(s) 1210, 1932, 2241, 2873
EcoT38I GRGCYC 1 cut(s) 2704
EgeI GGCGCC 1 cut(s) 1475
EheI GGCGCC 1 cut(s) 1475
ErhI CCWWGG 4 cut(s) 1210, 1932, 2241, 2873
FalI AAGNNNNNCTT 2 cut(s) 2479, 2511
FaqI GGGAC 5 cut(s) 1179, 1192, 1494, 1907, 2643
FbaI TGATCA 3 cut(s) 235, 685, 2665
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 6 cut(s) 263, 1653, 1961, 2654, 2657, 2722
FokI GGATG 4 cut(s) 239, 1805, 1876, 2089
FriOI GRGCYC 1 cut(s) 2704
Fsp4HI GCNGC 6 cut(s) 263, 1653, 1961, 2654, 2657, 2722
FspI TGCGCA 1 cut(s) 1970
GlaI GCGC 3 cut(s) 1475, 1970, 2239
GluI GCNGC 6 cut(s) 263, 1653, 1961, 2654, 2657, 2722
GsuI CTGGAG 2 cut(s) 475, 2156
HaeII RGCGCY 2 cut(s) 1477, 2241
HaeIII GGCC 4 cut(s) 100, 249, 1492, 2269
HapII CCGG 4 cut(s) 93, 246, 842, 2484
HhaI GCGC 3 cut(s) 1476, 1971, 2240
Hin1I GRCGYC 1 cut(s) 1474
Hin6I GCGC 3 cut(s) 1474, 1969, 2238
HinP1I GCGC 3 cut(s) 1474, 1969, 2238
HincII GTYRAC 1 cut(s) 2035
HindII GTYRAC 1 cut(s) 2035
HindIII AAGCTT 2 cut(s) 1225, 1531
HpaII CCGG 4 cut(s) 93, 246, 842, 2484
HphI GGTGA 6 cut(s) 1133, 1298, 1970, 2473, 2570, 2746
Hpy166II GTNNAC 8 cut(s) 497, 680, 851, 1289, 1391, 2035, 2783, 2866
Hpy8I GTNNAC 8 cut(s) 497, 680, 851, 1289, 1391, 2035, 2783, 2866
Hpy99I CGWCG 1 cut(s) 2684
HpyAV CCTTC 9 cut(s) 191, 336, 529, 557, 657, 1328, 2123, 2154, 2505
HpyCH4III ACNGT 9 cut(s) 209, 752, 833, 1859, 2015, 2520, 2752, 2780, 2895
HpyCH4IV ACGT 2 cut(s) 1117, 2191
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 1498, 1966, 2682
HpySE526I ACGT 2 cut(s) 1117, 2191
Hsp92I GRCGYC 1 cut(s) 1474
HspAI GCGC 3 cut(s) 1474, 1969, 2238
KasI GGCGCC 1 cut(s) 1473
Ksp22I TGATCA 3 cut(s) 235, 685, 2665
LmnI GCTCC 6 cut(s) 648, 1660, 2664, 2699, 2707, 2877
Lsp1109I GCAGC 6 cut(s) 249, 1639, 1947, 2640, 2668, 2733
LweI GCATC 5 cut(s) 355, 623, 756, 903, 2210
MaeII ACGT 2 cut(s) 1117, 2191
MaeIII GTNAC 5 cut(s) 631, 885, 2332, 2454, 2597
MboII GAAGA 9 cut(s) 206, 797, 1768, 1808, 2438, 2499, 2801, 2818, 2876
MfeI CAATTG 2 cut(s) 1460, 1622
MflI RGATCY 3 cut(s) 151, 1760, 2418
MhlI GDGCHC 2 cut(s) 1393, 2704
MlsI TGGCCA 2 cut(s) 1492, 2269
MluNI TGGCCA 2 cut(s) 1492, 2269
Mly113I GGCGCC 1 cut(s) 1474
MlyI GAGTC 3 cut(s) 961, 1097, 2605
MmeI TCCRAC 7 cut(s) 197, 1326, 1589, 1707, 1841, 2038, 2800
Mox20I TGGCCA 2 cut(s) 1492, 2269
MroXI GAANNNNTTC 3 cut(s) 1040, 1382, 2494
MscI TGGCCA 2 cut(s) 1492, 2269
MslI CAYNNNNRTG 2 cut(s) 255, 1202
Msp20I TGGCCA 2 cut(s) 1492, 2269
MspA1I CMGCKG 1 cut(s) 2653
MspCI CTTAAG 1 cut(s) 1148
MspI CCGG 4 cut(s) 93, 246, 842, 2484
MspR9I CCNGG 4 cut(s) 93, 725, 843, 1478
MunI CAATTG 2 cut(s) 1460, 1622
Mva1269I GAATGC 2 cut(s) 2326, 2880
MvaI CCWGG 2 cut(s) 725, 1478
MwoI GCNNNNNNNGC 4 cut(s) 117, 1498, 1966, 2682
NarI GGCGCC 1 cut(s) 1474
NciI CCSGG 2 cut(s) 93, 843
NcoI CCATGG 3 cut(s) 1932, 2241, 2873
NlaIV GGNNCC 5 cut(s) 162, 1195, 1475, 1482, 1681
NmeAIII GCCGAG 1 cut(s) 795
NmuCI GTSAC 3 cut(s) 885, 2454, 2597
NsbI TGCGCA 1 cut(s) 1970
NspI RCATGY 1 cut(s) 2872
NspV TTCGAA 1 cut(s) 2120
PaeR7I CTCGAG 3 cut(s) 155, 1085, 1823
PcsI WCGNNNNNNNCGW 1 cut(s) 2685
PctI GAATGC 2 cut(s) 2326, 2880
PdmI GAANNNNTTC 3 cut(s) 1040, 1382, 2494
PfeI GAWTC 8 cut(s) 1466, 1606, 1636, 1884, 2258, 2365, 2525, 2823
PflMI CCANNNNNTGG 2 cut(s) 924, 2179
PkrI GCNGC 6 cut(s) 264, 1654, 1962, 2655, 2658, 2723
PleI GAGTC 3 cut(s) 960, 1096, 2604
PluTI GGCGCC 1 cut(s) 1477
PpsI GAGTC 3 cut(s) 960, 1096, 2604
Ppu21I YACGTR 2 cut(s) 1118, 2192
PpuMI RGGWCCY 3 cut(s) 160, 1193, 1481
PsiI TTATAA 1 cut(s) 1323
Psp124BI GAGCTC 1 cut(s) 2704
Psp5II RGGWCCY 3 cut(s) 160, 1193, 1481
Psp6I CCWGG 2 cut(s) 723, 1476
PspGI CCWGG 2 cut(s) 723, 1476
PspN4I GGNNCC 5 cut(s) 162, 1195, 1475, 1482, 1681
PspPI GGNCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
PspPPI RGGWCCY 3 cut(s) 160, 1193, 1481
PstI CTGCAG 1 cut(s) 2658
PstNI CAGNNNCTG 1 cut(s) 2899
PsuI RGATCY 3 cut(s) 151, 1760, 2418
PvuII CAGCTG 1 cut(s) 2653
RsaI GTAC 8 cut(s) 385, 517, 1543, 1558, 1628, 1851, 1985, 2017
RsaNI GTAC 8 cut(s) 384, 516, 1542, 1557, 1627, 1850, 1984, 2016
RseI CAYNNNNRTG 2 cut(s) 255, 1202
SacI GAGCTC 1 cut(s) 2704
SatI GCNGC 6 cut(s) 263, 1653, 1961, 2654, 2657, 2722
Sau96I GGNCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
ScaI AGTACT 2 cut(s) 517, 1558
SchI GAGTC 3 cut(s) 961, 1097, 2605
ScrFI CCNGG 4 cut(s) 93, 725, 843, 1478
SduI GDGCHC 2 cut(s) 1393, 2704
SfaNI GCATC 5 cut(s) 355, 623, 756, 903, 2210
SfcI CTRYAG 1 cut(s) 2654
SfoI GGCGCC 1 cut(s) 1475
Sfr274I CTCGAG 3 cut(s) 155, 1085, 1823
SfuI TTCGAA 1 cut(s) 2120
SinI GGWCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
SlaI CTCGAG 3 cut(s) 155, 1085, 1823
SmiMI CAYNNNNRTG 2 cut(s) 255, 1202
SmlI CTYRAG 4 cut(s) 155, 1085, 1148, 1823
SmoI CTYRAG 4 cut(s) 155, 1085, 1148, 1823
SpeI ACTAGT 2 cut(s) 1007, 1418
SsiI CCGC 4 cut(s) 104, 2476, 2697, 2741
SspDI GGCGCC 1 cut(s) 1473
SstI GAGCTC 1 cut(s) 2704
StyD4I CCNGG 4 cut(s) 91, 723, 841, 1476
StyI CCWWGG 4 cut(s) 1210, 1932, 2241, 2873
TaaI ACNGT 9 cut(s) 209, 752, 833, 1859, 2015, 2520, 2752, 2780, 2895
TaiI ACGT 2 cut(s) 1120, 2194
TatI WGTACW 3 cut(s) 383, 515, 1556
TfiI GAWTC 8 cut(s) 1466, 1606, 1636, 1884, 2258, 2365, 2525, 2823
TscAI CASTG 4 cut(s) 557, 1393, 1699, 1846
TseFI GTSAC 3 cut(s) 885, 2454, 2597
TseI GCWGC 6 cut(s) 262, 1652, 1960, 2653, 2656, 2721
Tsp45I GTSAC 3 cut(s) 885, 2454, 2597
TspGWI ACGGA 3 cut(s) 2001, 2251, 2778
TspRI CASTG 4 cut(s) 557, 1393, 1699, 1846
Van91I CCANNNNNTGG 2 cut(s) 924, 2179
Vha464I CTTAAG 1 cut(s) 1148
VneI GTGCAC 1 cut(s) 1389
VpaK11BI GGWCC 7 cut(s) 160, 361, 833, 1193, 1481, 2182, 2389
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 1 cut(s) 2872
XcmI CCANNNNNNNNNTGG 2 cut(s) 1572, 1778
XhoI CTCGAG 3 cut(s) 155, 1085, 1823
XmaJI CCTAGG 1 cut(s) 1210
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 3 cut(s) 1040, 1382, 2494
ZrmI AGTACT 2 cut(s) 517, 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.