pycom08g20750

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000281
Physical Location & Seq
Forward (+)
20422803 .. 20425574
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20750.2

Sequence Viewer

Length: 2700 bp
ATGGATAGGCCTTTGAGACTTGTTTTACTTGTGAGTTTCCTGAGCATTGCAGCCACTCCCATTAGTTTGGGCAATGCAAATGTGGGACTTCAGGTTCCTTGCAAAGAAAATGAGAGGCAGGCCCTTCTGATGTTCAAGCAAGGACTTGTTAATTCTTCGAACAGGCTTTCGTCCTGGATTGGCGAAGGGGATTGTTGCTTTTGGCCTGGAGTTGTCTGCAGTAACTTAACCGGCCATGTCCGCGCACTCCACCTTGGTAATTACTATTTGGGTGGAATGCAGATTCCCAACTTCTTCGGTTCTCTTACAAGTTTAAGACATCTTGACCTCTCACAATCAGATTTCCAGGGAATAATTCCTCATCAGCTGGGGAATCTTTCTAATCTACGCTTTCTCGACGTCCATGGTAACTATTTTGAGGTTAAGAGCCTGCAATGGATTTCTGGTCTTCCTCAGCTGCAGCACCTGGACATGAGTGGTGTAGATCTCAGAGAAGCATCCGATTTGTTCCAGGTGACAAACACTTTCCCCTCTTTGCTAGAGTACTTGAATATGTCCGATTGCGGACTATATCAAATACCATTTGGAATTGCAAATGCTTCTGTTCTTAAAGTTCTTAATCTCGAGGGGAATTCCATCAATTCTACCATACCAAAATGGTTGTACAGCCTTAGCCATCTGGAGTCACTATTTCTTTCCTACAATCACTTGCATGGCCAAATCTCAAGCTCCATTGCAAACTTAGCAGACATTGTCAATCTTGACTTATTTCATAATCAACTTGAAGGGGAACTCCCAACCTCAATGGGAAATCTTTGCAAGTTGACGGTTTTTGATCTGTCGAGGAACCGTGTCAATGGGAGGGTATCCGAAATCTTTGAAAGTTTGTCTAGGTGCAATTCAAGTCAGCTAGAGTCTTTGAGCTTATCCAATAATAATCTTTCTGGTCTTTTACCAGATGAGCTAGGAAATTTTGAAAAATTACGTTTCCTTGATCTTTCAAGCAATTCAACATCAGGTTCCATTCCAATGTCCTTAGGAAATCTGTCATGCTTAGAAGAATTACGCATTCAGAATAATTCATTCAAGGGTGTCGTCTCTGAAGTTCATTTTACCAATCTTACAAAATTGGTGAAACTTTATGCAAAAAATAATTTATTGAAGCTGGAAACCAGTAGAGATTGGCATCCACCATTTCAACTCCGAGTGTTGTTCTTAGATTCTTGGTATCTAGGGCCAGAGTTACCTTTGTGGTTTCAGAGACAAACACAATTGCAGTATCTAAGCATGGTCAACACTAGCATTTCAGGTACCATCCCAACCTGGTTTTGGAACTTTTCTTCCCATCTAAGATTTGTCGATCTCTCTCACAATCAATTGTATGGGGAAGTTCCAAATATAGTTGGTGCTCCTTTAGACATAATTGACCTAAGTTCAAACCAGTTCAATGGTTCGTTACCTCTCGTCTCCTCCACCGTTGATGTACTAGATCTTTCCAATTCAACATTTTCCGGATCCATCTTTCACTTCTTTTGTGATAGCATAGATGCGCCAAAACAACTCAGAATTCTTTATCTTGAGAACAACTTTCTTGATGGACCAATTCCTGATTGTTGGCAAAACTGGAAAAAGTTGATTGTTCTAAATTTAGAGAACAACAATTTGACAGGGAACATTCCAAGCTCCATAGGATACTTACTTTTCCTCGAATCGCTGCACTTGCGCAATAATCACCTGTTTGGAGAGTTGCCTATGTCCCTACAGAATTGTCAGAACCTGTTAGTTGCTGACCTTGGCGAAAATAAGTTTGTTGGAAGCATACCGATGTGGATAGGAGAAAGTCTTTCAAATTTGATCGTTCTCATCCTTCGTTCCAATAAGCTTCAGAGTGACATTCCTAACGAAATCTGCAGTCTTGTCAATCTCCAAATCTTGGACCTTGCGCATAATAATCTCTCCGGAACAATCCCAAAATGTTTCAACAATTTCGGTGCCATGGCCACCTTGTCAAACTCAGGAGGTCCCATTTCATTCTTCAGTTATATGTACGGTTCGCCTGAGCAGTACATGGAGGAAGCAATCTTGGTGAGAAAAGGCAGAACAGACAAATATAGCAAATCGCTTAGCTTGGTAGCTAACTTGGACCTTTCACACAACATGATATCTGGAGAGATTCCCGAGGAATTGACCAGCCTTATCAGCTTGCAATCATTGAACATATCGAAAAATCTATTGACCGGAAAAATCCCTTCAATGATTGGTGATATGGTAATGTTAGAGTCTCTTGATCTTTCCATGAACAAACTTTCTGGTGAAATTTCTCCAAGCATGTCGAGGTTGACATTTCTTGGTACCTTGAATTTGTCTTATAACAATTTGACCGGACAGATTCCCCAATGCACACAGCTCGGGGGCTTTGATCAGTACAGCTATATTGGCAATGAACTTTGCGGATTTCCACTCAACGTGAGCTGCAAGGAAAATCGGACAATGCAGCCAGTAGTTGTTCAAAACCACATGGGTGGCCATTTACTCGAAGACAGTTGGTTCTATTTGAGCTTGGGACTAGGCTTTCTGTTCGGTTTTTGGGGTGTTCTTGGTTCGTTGCTTCTCAACATGCCATGGAGCTTTCGCTTTTCTCAGTTCCAGAATAGTATTGTTCGTAGACTATATGGTGTAATTGTGGAATATTGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

900

Amino Acids

100.42

Weight (kDa)

5.46

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2370
AatII GACGTC 1 cut(s) 402
Acc16I TGCGCA 2 cut(s) 1724, 1944
Acc65I GGTACC 2 cut(s) 1310, 2351
AccB1I GGYRCC 3 cut(s) 1310, 1991, 2351
AccB7I CCANNNNNTGG 1 cut(s) 1933
AccI GTMKAC 1 cut(s) 2665
AccII CGCG 1 cut(s) 243
AccIII TCCGGA 2 cut(s) 1511, 1958
AciI CCGC 3 cut(s) 241, 564, 2451
AclWI GGATC 2 cut(s) 1509, 1522
AcoI YGGCCR 4 cut(s) 232, 715, 1998, 2524
AcsI RAATTY 7 cut(s) 631, 970, 1566, 1645, 1849, 2316, 2359
AcuI CTGAAG 4 cut(s) 74, 1122, 1868, 2020
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 8 cut(s) 545, 665, 1312, 1485, 2048, 2066, 2353, 2426
AfiI CCNNNNNNNGG 2 cut(s) 1329, 1933
AjnI CCWGG 6 cut(s) 173, 205, 345, 465, 510, 1322
AleI CACNNNNGTG 1 cut(s) 2520
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 1325, 1357
Alw21I GWGCWC 1 cut(s) 1411
Alw26I GTCTC 5 cut(s) 10, 1102, 1255, 1471, 2286
AlwI GGATC 2 cut(s) 1509, 1522
AlwNI CAGNNNCTG 2 cut(s) 466, 1777
Ama87I CYCGRG 3 cut(s) 623, 2177, 2408
Aor13HI TCCGGA 2 cut(s) 1511, 1958
AoxI GGCC 8 cut(s) 8, 120, 203, 232, 715, 1235, 1998, 2524
ApeKI GCWGC 6 cut(s) 50, 457, 460, 1714, 2472, 2494
ApoI RAATTY 7 cut(s) 631, 970, 1566, 1645, 1849, 2316, 2359
Asp718I GGTACC 2 cut(s) 1310, 2351
AspLEI GCGC 4 cut(s) 245, 1552, 1725, 1945
AspS9I GGNCC 6 cut(s) 121, 1235, 1598, 1936, 2021, 2143
AsuHPI GGTGA 6 cut(s) 526, 1144, 1724, 2098, 2273, 2324
AsuII TTCGAA 1 cut(s) 158
AvaI CYCGRG 3 cut(s) 623, 2177, 2408
AvaII GGWCC 4 cut(s) 1598, 1936, 2021, 2143
AxyI CCTNAGG 1 cut(s) 1036
BalI TGGCCA 3 cut(s) 717, 2000, 2526
BamHI GGATCC 1 cut(s) 1514
BanI GGYRCC 3 cut(s) 1310, 1991, 2351
BbsI GAAGAC 2 cut(s) 440, 2544
Bbv12I GWGCWC 1 cut(s) 1411
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 6 cut(s) 62, 444, 472, 1701, 2459, 2506
BccI CCATC 6 cut(s) 644, 684, 1322, 1353, 1526, 1589
BcgI CGANNNNNNTGC 2 cut(s) 2389, 2423
BciT130I CCWGG 6 cut(s) 175, 207, 347, 467, 512, 1324
BciVI GTATCC 2 cut(s) 877, 1685
BclI TGATCA 1 cut(s) 2419
BcoDI GTCTC 5 cut(s) 10, 1102, 1255, 1471, 2286
BfaI CTAG 8 cut(s) 539, 891, 911, 965, 1232, 1299, 1487, 2567
BfmI CTRYAG 4 cut(s) 217, 458, 1760, 1909
BfuI GTATCC 2 cut(s) 877, 1685
BglII AGATCT 2 cut(s) 484, 1489
BisI GCNGC 6 cut(s) 51, 458, 461, 1715, 2473, 2495
BlpI GCTNAGC 1 cut(s) 2123
BlsI GCNGC 6 cut(s) 52, 459, 462, 1716, 2474, 2496
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 6 cut(s) 175, 207, 347, 467, 512, 1324
Bme18I GGWCC 4 cut(s) 1598, 1936, 2021, 2143
BmeT110I CYCGRG 3 cut(s) 623, 2177, 2408
BmgT120I GGNCC 6 cut(s) 121, 1235, 1598, 1936, 2021, 2143
BmiI GGNNCC 8 cut(s) 96, 848, 1021, 1312, 1516, 1993, 2023, 2353
BmrFI CCNGG 6 cut(s) 175, 207, 347, 467, 512, 1324
BmsI GCATC 3 cut(s) 506, 1195, 1537
BpiI GAAGAC 2 cut(s) 440, 2544
BpmI CTGGAG 3 cut(s) 228, 701, 2187
Bpu10I CCTNAGC 4 cut(s) 41, 453, 671, 2058
Bpu1102I GCTNAGC 1 cut(s) 2123
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 709, 1598
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 7 cut(s) 253, 346, 403, 1792, 1995, 2178, 2621
BsaWI WCCGGW 4 cut(s) 1511, 1958, 2237, 2381
Bsc4I CCNNNNNNNGG 2 cut(s) 1329, 1933
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 4 cut(s) 1173, 1441, 1628, 2498
Bse21I CCTNAGG 1 cut(s) 1036
Bse3DI GCAATG 5 cut(s) 45, 79, 440, 732, 2446
Bse8I GATNNNNATC 1 cut(s) 1185
BseAI TCCGGA 2 cut(s) 1511, 1958
BseBI CCWGG 6 cut(s) 175, 207, 347, 467, 512, 1324
BseDI CCNNGG 7 cut(s) 253, 346, 403, 1792, 1995, 2178, 2621
BseGI GGATG 4 cut(s) 497, 1186, 1314, 1863
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 2 cut(s) 1329, 1933
BseMI GCAATG 5 cut(s) 45, 79, 440, 732, 2446
BseMII CTCAG 7 cut(s) 32, 467, 502, 1576, 2028, 2049, 2654
BseNI ACTGG 4 cut(s) 1173, 1441, 1628, 2498
BseRI GAGGAG 1 cut(s) 1459
BseXI GCAGC 6 cut(s) 62, 444, 472, 1701, 2459, 2506
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 1700
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 8 cut(s) 10, 122, 205, 234, 717, 1237, 2000, 2526
BshNI GGYRCC 3 cut(s) 1310, 1991, 2351
BsiHKAI GWGCWC 1 cut(s) 1411
BsiHKCI CYCGRG 3 cut(s) 623, 2177, 2408
BsiSI CCGG 5 cut(s) 231, 1512, 1959, 2238, 2382
BslFI GGGAC 4 cut(s) 99, 1741, 2007, 2577
BslI CCNNNNNNNGG 2 cut(s) 1329, 1933
BsmAI GTCTC 5 cut(s) 10, 1102, 1255, 1471, 2286
BsmBI CGTCTC 2 cut(s) 1102, 1471
BsmFI GGGAC 4 cut(s) 99, 1741, 2007, 2577
BsmI GAATGC 2 cut(s) 282, 1068
BsnI GGCC 8 cut(s) 10, 122, 205, 234, 717, 1237, 2000, 2526
BsoBI CYCGRG 3 cut(s) 623, 2177, 2408
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 1411
Bsp13I TCCGGA 2 cut(s) 1511, 1958
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
Bsp1720I GCTNAGC 1 cut(s) 2123
Bsp19I CCATGG 3 cut(s) 403, 1995, 2621
BspACI CCGC 3 cut(s) 241, 564, 2451
BspANI GGCC 8 cut(s) 10, 122, 205, 234, 717, 1237, 2000, 2526
BspCNI CTCAG 7 cut(s) 33, 466, 501, 1575, 2027, 2050, 2653
BspEI TCCGGA 2 cut(s) 1511, 1958
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 8 cut(s) 96, 848, 1021, 1312, 1516, 1993, 2023, 2353
BspMAI CTGCAG 3 cut(s) 221, 462, 1913
BspPI GGATC 2 cut(s) 1509, 1522
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 3 cut(s) 1310, 1991, 2351
BsrDI GCAATG 5 cut(s) 45, 79, 440, 732, 2446
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 4 cut(s) 1173, 1441, 1628, 2498
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 7 cut(s) 253, 346, 403, 1792, 1995, 2178, 2621
BssMI GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 5 cut(s) 253, 403, 1792, 1995, 2621
Bst2UI CCWGG 6 cut(s) 175, 207, 347, 467, 512, 1324
Bst4CI ACNGT 5 cut(s) 829, 851, 1477, 2051, 2543
BstACI GRCGYC 1 cut(s) 399
BstAUI TGTACA 1 cut(s) 663
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 120, 431, 2204
BstDSI CCRYGG 3 cut(s) 403, 1995, 2621
BstF5I GGATG 4 cut(s) 497, 1186, 1314, 1863
BstFNI CGCG 1 cut(s) 243
BstHHI GCGC 4 cut(s) 245, 1552, 1725, 1945
BstKTI GATC 9 cut(s) 487, 838, 997, 1363, 1492, 1517, 1857, 2290, 2422
BstMAI GTCTC 5 cut(s) 10, 1102, 1255, 1471, 2286
BstMBI GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
BstMWI GCNNNNNNNGC 5 cut(s) 240, 743, 1720, 2199, 2436
BstNI CCWGG 6 cut(s) 175, 207, 347, 467, 512, 1324
BstNSI RCATGY 2 cut(s) 2332, 2620
BstSCI CCNGG 6 cut(s) 173, 205, 345, 465, 510, 1322
BstSFI CTRYAG 4 cut(s) 217, 458, 1760, 1909
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 6 cut(s) 62, 444, 472, 1701, 2459, 2506
BstV2I GAAGAC 2 cut(s) 440, 2544
BstX2I RGATCY 3 cut(s) 484, 1489, 1514
BstXI CCANNNNNNTGG 3 cut(s) 67, 1448, 2522
BstYI RGATCY 3 cut(s) 484, 1489, 1514
Bsu36I CCTNAGG 1 cut(s) 1036
BsuI GTATCC 2 cut(s) 877, 1685
BsuRI GGCC 8 cut(s) 10, 122, 205, 234, 717, 1237, 2000, 2526
BtgI CCRYGG 3 cut(s) 403, 1995, 2621
BtsCI GGATG 4 cut(s) 497, 1186, 1314, 1863
Cac8I GCNNGC 3 cut(s) 120, 431, 2204
CaiI CAGNNNCTG 2 cut(s) 466, 1777
CfoI GCGC 4 cut(s) 245, 1552, 1725, 1945
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 6 cut(s) 121, 1235, 1598, 1936, 2021, 2143
CsiI ACCWGGT 1 cut(s) 1322
Csp6I GTAC 8 cut(s) 544, 664, 1311, 1484, 2047, 2065, 2352, 2425
CviQI GTAC 8 cut(s) 544, 664, 1311, 1484, 2047, 2065, 2352, 2425
DpnI GATC 9 cut(s) 486, 837, 996, 1362, 1491, 1516, 1856, 2289, 2421
DpnII GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
EaeI YGGCCR 4 cut(s) 232, 715, 1998, 2524
Eco130I CCWWGG 5 cut(s) 253, 403, 1792, 1995, 2621
Eco147I AGGCCT 1 cut(s) 10
Eco32I GATATC 1 cut(s) 2163
Eco47I GGWCC 4 cut(s) 1598, 1936, 2021, 2143
Eco57I CTGAAG 4 cut(s) 74, 1122, 1868, 2020
Eco81I CCTNAGG 1 cut(s) 1036
Eco88I CYCGRG 3 cut(s) 623, 2177, 2408
EcoO109I RGGNCCY 2 cut(s) 121, 2021
EcoRI GAATTC 2 cut(s) 631, 1566
EcoRII CCWGG 6 cut(s) 173, 205, 345, 465, 510, 1322
EcoRV GATATC 1 cut(s) 2163
EcoT14I CCWWGG 5 cut(s) 253, 403, 1792, 1995, 2621
ErhI CCWWGG 5 cut(s) 253, 403, 1792, 1995, 2621
Esp3I CGTCTC 2 cut(s) 1102, 1471
FaqI GGGAC 4 cut(s) 99, 1741, 2007, 2577
FbaI TGATCA 1 cut(s) 2419
FblI GTMKAC 1 cut(s) 2665
Fnu4HI GCNGC 6 cut(s) 51, 458, 461, 1715, 2473, 2495
FokI GGATG 4 cut(s) 484, 1173, 1301, 1850
Fsp4HI GCNGC 6 cut(s) 51, 458, 461, 1715, 2473, 2495
FspBI CTAG 8 cut(s) 539, 891, 911, 965, 1232, 1299, 1487, 2567
FspI TGCGCA 2 cut(s) 1724, 1944
GlaI GCGC 4 cut(s) 244, 1551, 1724, 1944
GluI GCNGC 6 cut(s) 51, 458, 461, 1715, 2473, 2495
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 3 cut(s) 228, 701, 2187
HaeIII GGCC 8 cut(s) 10, 122, 205, 234, 717, 1237, 2000, 2526
HapII CCGG 5 cut(s) 231, 1512, 1959, 2238, 2382
HhaI GCGC 4 cut(s) 245, 1552, 1725, 1945
Hin1I GRCGYC 1 cut(s) 399
Hin6I GCGC 4 cut(s) 243, 1550, 1723, 1943
HinP1I GCGC 4 cut(s) 243, 1550, 1723, 1943
HincII GTYRAC 3 cut(s) 825, 1294, 2340
HindII GTYRAC 3 cut(s) 825, 1294, 2340
HindIII AAGCTT 1 cut(s) 1880
HinfI GANTC 9 cut(s) 283, 373, 683, 914, 1220, 1709, 2173, 2279, 2389
HpaII CCGG 5 cut(s) 231, 1512, 1959, 2238, 2382
HphI GGTGA 6 cut(s) 526, 1144, 1724, 2098, 2273, 2324
Hpy166II GTNNAC 4 cut(s) 825, 1294, 2340, 2666
Hpy8I GTNNAC 4 cut(s) 825, 1294, 2340, 2666
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 5 cut(s) 134, 179, 779, 1877, 2259
HpyCH4III ACNGT 5 cut(s) 829, 851, 1477, 2051, 2543
HpyCH4IV ACGT 3 cut(s) 399, 985, 2466
HpyF10VI GCNNNNNNNGC 5 cut(s) 240, 743, 1720, 2199, 2436
HpySE526I ACGT 3 cut(s) 399, 985, 2466
Hsp92I GRCGYC 1 cut(s) 399
HspAI GCGC 4 cut(s) 243, 1550, 1723, 1943
Kpn2I TCCGGA 2 cut(s) 1511, 1958
KpnI GGTACC 2 cut(s) 1314, 2355
Ksp22I TGATCA 1 cut(s) 2419
Kzo9I GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
LmnI GCTCC 4 cut(s) 734, 1414, 1688, 2625
Lsp1109I GCAGC 6 cut(s) 62, 444, 472, 1701, 2459, 2506
LweI GCATC 3 cut(s) 506, 1195, 1537
MabI ACCWGGT 1 cut(s) 1322
MaeI CTAG 8 cut(s) 539, 891, 911, 965, 1232, 1299, 1487, 2567
MaeII ACGT 3 cut(s) 399, 985, 2466
MaeIII GTNAC 7 cut(s) 221, 407, 514, 684, 1242, 1455, 1889
MalI GATC 9 cut(s) 486, 837, 996, 1362, 1491, 1516, 1856, 2289, 2421
MboI GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
MboII GAAGA 7 cut(s) 147, 286, 440, 1070, 1332, 2026, 2549
MfeI CAATTG 2 cut(s) 1271, 1376
MflI RGATCY 3 cut(s) 484, 1489, 1514
MhlI GDGCHC 1 cut(s) 1411
MlsI TGGCCA 3 cut(s) 717, 2000, 2526
MluNI TGGCCA 3 cut(s) 717, 2000, 2526
MlyI GAGTC 3 cut(s) 692, 923, 2288
MmeI TCCRAC 1 cut(s) 1792
Mox20I TGGCCA 3 cut(s) 717, 2000, 2526
MroI TCCGGA 2 cut(s) 1511, 1958
MscI TGGCCA 3 cut(s) 717, 2000, 2526
MseI TTAA 6 cut(s) 150, 227, 314, 423, 609, 618
MslI CAYNNNNRTG 4 cut(s) 711, 1028, 1823, 2520
Msp20I TGGCCA 3 cut(s) 717, 2000, 2526
MspA1I CMGCKG 2 cut(s) 367, 457
MspI CCGG 5 cut(s) 231, 1512, 1959, 2238, 2382
MspR9I CCNGG 6 cut(s) 175, 207, 347, 467, 512, 1324
MunI CAATTG 2 cut(s) 1271, 1376
Mva1269I GAATGC 2 cut(s) 282, 1068
MvaI CCWGG 6 cut(s) 175, 207, 347, 467, 512, 1324
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 5 cut(s) 240, 743, 1720, 2199, 2436
NcoI CCATGG 3 cut(s) 403, 1995, 2621
NdeII GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
NlaIV GGNNCC 8 cut(s) 96, 848, 1021, 1312, 1516, 1993, 2023, 2353
NmuCI GTSAC 3 cut(s) 514, 684, 1889
NsbI TGCGCA 2 cut(s) 1724, 1944
NspI RCATGY 2 cut(s) 2332, 2620
NspV TTCGAA 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 2520
PaeR7I CTCGAG 1 cut(s) 623
PceI AGGCCT 1 cut(s) 10
PctI GAATGC 2 cut(s) 282, 1068
PfeI GAWTC 6 cut(s) 283, 373, 1220, 1709, 2173, 2389
PflFI GACNNNGTC 1 cut(s) 752
PflMI CCANNNNNTGG 1 cut(s) 1933
PfoI TCCNGGA 1 cut(s) 173
PkrI GCNGC 6 cut(s) 52, 459, 462, 1716, 2474, 2496
PleI GAGTC 3 cut(s) 691, 922, 2287
PpsI GAGTC 3 cut(s) 691, 922, 2287
PpuMI RGGWCCY 1 cut(s) 2021
PsiI TTATAA 1 cut(s) 2370
Psp5II RGGWCCY 1 cut(s) 2021
Psp6I CCWGG 6 cut(s) 173, 205, 345, 465, 510, 1322
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 6 cut(s) 173, 205, 345, 465, 510, 1322
PspN4I GGNNCC 8 cut(s) 96, 848, 1021, 1312, 1516, 1993, 2023, 2353
PspPI GGNCC 6 cut(s) 121, 1235, 1598, 1936, 2021, 2143
PspPPI RGGWCCY 1 cut(s) 2021
PstI CTGCAG 3 cut(s) 221, 462, 1913
PstNI CAGNNNCTG 2 cut(s) 466, 1777
PsuI RGATCY 3 cut(s) 484, 1489, 1514
PsyI GACNNNGTC 1 cut(s) 752
PvuII CAGCTG 2 cut(s) 367, 457
RsaI GTAC 8 cut(s) 545, 665, 1312, 1485, 2048, 2066, 2353, 2426
RsaNI GTAC 8 cut(s) 544, 664, 1311, 1484, 2047, 2065, 2352, 2425
RseI CAYNNNNRTG 4 cut(s) 711, 1028, 1823, 2520
SaqAI TTAA 6 cut(s) 150, 227, 314, 423, 609, 618
SatI GCNGC 6 cut(s) 51, 458, 461, 1715, 2473, 2495
Sau3AI GATC 9 cut(s) 484, 835, 994, 1360, 1489, 1514, 1854, 2287, 2419
Sau96I GGNCC 6 cut(s) 121, 1235, 1598, 1936, 2021, 2143
ScaI AGTACT 1 cut(s) 545
SchI GAGTC 3 cut(s) 692, 923, 2288
ScrFI CCNGG 6 cut(s) 175, 207, 347, 467, 512, 1324
SduI GDGCHC 1 cut(s) 1411
SexAI ACCWGGT 1 cut(s) 1322
SfaNI GCATC 3 cut(s) 506, 1195, 1537
SfcI CTRYAG 4 cut(s) 217, 458, 1760, 1909
Sfr274I CTCGAG 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 158
SinI GGWCC 4 cut(s) 1598, 1936, 2021, 2143
SlaI CTCGAG 1 cut(s) 623
SmiMI CAYNNNNRTG 4 cut(s) 711, 1028, 1823, 2520
SmlI CTYRAG 3 cut(s) 623, 724, 1577
SmoI CTYRAG 3 cut(s) 623, 724, 1577
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 241, 564, 2451
SspI AATATT 1 cut(s) 2690
SspMI CTAG 8 cut(s) 539, 891, 911, 965, 1232, 1299, 1487, 2567
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 6 cut(s) 173, 205, 345, 465, 510, 1322
StyI CCWWGG 5 cut(s) 253, 403, 1792, 1995, 2621
TaaI ACNGT 5 cut(s) 829, 851, 1477, 2051, 2543
TaiI ACGT 3 cut(s) 402, 988, 2469
TaqI TCGA 9 cut(s) 158, 396, 624, 842, 1359, 1707, 2222, 2333, 2535
TatI WGTACW 5 cut(s) 543, 663, 1483, 2064, 2424
TfiI GAWTC 6 cut(s) 283, 373, 1220, 1709, 2173, 2389
Tru1I TTAA 6 cut(s) 150, 227, 314, 423, 609, 618
Tru9I TTAA 6 cut(s) 150, 227, 314, 423, 609, 618
TseFI GTSAC 3 cut(s) 514, 684, 1889
TseI GCWGC 6 cut(s) 50, 457, 460, 1714, 2472, 2494
Tsp45I GTSAC 3 cut(s) 514, 684, 1889
TspDTI ATGAA 6 cut(s) 761, 1071, 1097, 2019, 2312, 2457
Tth111I GACNNNGTC 1 cut(s) 752
Van91I CCANNNNNTGG 1 cut(s) 1933
VpaK11BI GGWCC 4 cut(s) 1598, 1936, 2021, 2143
XapI RAATTY 7 cut(s) 631, 970, 1566, 1645, 1849, 2316, 2359
XceI RCATGY 2 cut(s) 2332, 2620
XcmI CCANNNNNNNNNTGG 1 cut(s) 1326
XhoI CTCGAG 1 cut(s) 623
XmiI GTMKAC 1 cut(s) 2665
XspI CTAG 8 cut(s) 539, 891, 911, 965, 1232, 1299, 1487, 2567
ZraI GACGTC 1 cut(s) 400
ZrmI AGTACT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.