MD01G1178700.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000281
Physical Location & Seq
Forward (+)
27871991 .. 27874378
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1178700.v1.1.491

Sequence Viewer

Length: 2388 bp
ATGGAGAGAACCATGAGAGTTGTTTTACTACTAATCAGGTTTCTAGCCATTGCAACCAGTACTTTCAGTATTGGTTTATGCAATGGAAATCCAGGTTGGCCTCCACTTTGCAAAGAGAGCGAAAGACGAGCACTTCTGATGTTCAAGCAAGATCTCAAGGACCCTGCCAATCAGCTTGCATCGTGGGTTGCAGAAGAAGGTTCAGACTGTTGTAGTTGGACAAGAGTAGTCTGTGATCACATGACCGGCCACATCCAGGAGCTGCACCTTAATAATACCAACCCTTATTGGGATTTCGACTCTTCCTTCGGTGGTAAGATAAATCCTTCTTTGCTCAGTTTAAAGCATCTCAACTTCTTGGACTTGAGTAACAATAATTTCAGTGGAACACAAATTCCTAGTTTCTTTGGTTCTATGACAAGTTTAACACACCTTAATCTTGCGTACTCATTGTTTGATGGAGTAATTCCTCATAAACTGGGAAATCTCTCCAGTCTGCGCCATCTCAATCTCAGTTTAAACGATCTGAAGGTTGAAAACCTTCAGTGGATTTCTGGTCTTTCTCTGCTGAAACACTTAGACTTGAGTTATGTAAATCTTAGCAAAGCATCTGACTGGTTGCAAGTTACAAACATGCTCCCTTCTTTGGTAGAGTTAGACATGTCCTATTGTCATCTTCATCAAATCCCCCCTCTACCCACCCCAAATTTTACTTCCCTGGTCGTCCTTGATCTTTCTAGAAACATTTTTAATTCTTTGATGCCGAGGTGGGTTTTCAGTCTTAAAAATCTAGTTTCTATTCATCTTAGTGCTTGTGGTTTCCAAGGTCCAATTCCTAGCATTTCACAGAATATCACATCTTTGAGGGAAATTGATTTGTCATCCAATTATATTAGTCTTGATCCGATTCCCAAATGGCTGTTTAACCAAAAAGATCTTGCATTGAGTCTAGAATCCAATGAACTTACAGGACAACTTCCAAGCATCATTCAGAATATGACTGGTCTTAAAGTTCTTGATCTCGGATGGAACGACTTCAATTCTACCATACCTGAATGGTTGTATAGCTTGAACAATCTCGAGTCTTTACTTCTTTCTTACAATGCCTTGCGTGGTGAAATATCGAGTTCCATTGGAAACATGACATCTCTTGTCAATCTTCACTTGGATGGTAATCAGTTGGAAGGGAAAATCCCAAATTCCTTGGGACATCTTTGTAAGTTGAAAGTTCTTGATCTGTCAGAGAACCATTTCACGGTTCGAAGACCATCCGAAATCTTCGAAAGTTTGTCCAAATGTGGTCCAGATGGAATAAAGTCATTGTCATTGAGGTATACTAATATATCAGGTCCCATTCCAATGTCACTAGGAAATCTGTCAAGCTTAGAAAAATTGTACATATCTGGAAATCATTTTAATGGAACTTTCACAGAAGTTATTGGTCAACTCAAAATGCTAATGGATTTGGAAATATCTTATAATTCGTTAGAAGGTGCAGTGTCGGAAGTGTCTTTTAGCAACCTTACAAAGTTGAAGCATTTCATTGCAAAAGGAAACTCATTTACTCTGAAAACCAGTCGAGATTGGGTTCCTCCTTTTCAACTTGAAATTTTGCAGCTGGATTCCTGGCATTTGGGGCCTGAATGGCCAATGTGGTTGCGGACACAAACGCAATTAAAAGAACTAAGCTTGTCTGGTACAGGAATTTCAAGTACTATTCCTACTTGGTTTTGGAACTTAACTTTCCATGTACAGTATCTGAATCTCTCTCGCAATCAATTGTATGGGCAGATTCAAAATATAGTTGCTGGTCCTCTTTCAACAGTTGATCTTGGTTCTAACCAATTCACTGGTGCATTGCCTATTGTTCCCACCTCAATAATGTGGCTAGATCTTTCCAATTCATCATTTTCTGGATCTGTTTTCCAGTTCTTCTGTGATAGGCCGGATGAACCAAAGCAACTCTATATTCTTCGTCTCGGGAACAATTTTCTCACTGGAAAAGTACCCGATTGTTGGATGAGTTGGCAATACTTGGTATTCCTAAATTTAGAAAACAACAACCTAACTGGGAATGTCCCAATGTCCATGGGATACTTGCAACACTTGGCATCGCTGCACTTGCGCAATAATCACCTGTACGGAGAATTGCCACATTCCCTGCAGAACTGTACCTCGTTGTCAGTTGTTGACCTTAGTGAAAATGGGTTTTCCGGAAGCATACCAATATGGATAGGGGAAAGCCTTTCAGGGTTGAATGTTCTTAACCTTCGTTCAAATAAGTTTGAAGGAGATATTCCTCATGAAGTTTGTTATTTGAAAAGTCTCCAGATATTGGACCTTGCACATAACAAACTCTCAGGAATGATACCAAGACGCTCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

796

Amino Acids

89.18

Weight (kDa)

6.0

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 79 5.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 112 - 209 1e-05 Leucine-rich repeat region
LRR_8 PF13855 116 - 176 1e-06 Leucine rich repeat
LRR_8 PF13855 141 - 197 1.3e-06 Leucine rich repeat
LRR_8 PF13855 315 - 370 8.3e-07 Leucine rich repeat
LRR_14 PF23598 322 - 445 2.5e-09 Leucine-rich repeat region
LRR_14 PF23598 451 - 564 2.1e-08 Leucine-rich repeat region
LRR_8 PF13855 461 - 520 6.2e-07 Leucine rich repeat
LRR_8 PF13855 726 - 786 6.4e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1479
Acc16I TGCGCA 1 cut(s) 2126
AccB7I CCANNNNNTGG 1 cut(s) 2335
AccI GTMKAC 1 cut(s) 1334
AccIII TCCGGA 1 cut(s) 2213
AciI CCGC 1 cut(s) 1660
AclWI GGATC 2 cut(s) 896, 1924
AcoI YGGCCR 2 cut(s) 247, 1646
AcsI RAATTY 6 cut(s) 393, 706, 1198, 1608, 1704, 2047
AcuI CTGAAG 2 cut(s) 527, 548
AfaI GTAC 9 cut(s) 61, 446, 1397, 1699, 1714, 1752, 2007, 2141, 2173
AfiI CCNNNNNNNGG 6 cut(s) 256, 289, 646, 1255, 2016, 2335
AflIII ACRYGT 1 cut(s) 660
AjnI CCWGG 4 cut(s) 91, 255, 717, 1625
AjuI GAANNNNNNNTTGG 6 cut(s) 79, 111, 697, 729, 816, 848
AloI GAACNNNNNNTCC 2 cut(s) 379, 411
AluBI AGCT 6 cut(s) 175, 262, 1068, 1383, 1618, 1689
AluI AGCT 6 cut(s) 175, 262, 1068, 1383, 1618, 1689
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 2 cut(s) 1982, 2330
AlwI GGATC 2 cut(s) 896, 1924
AlwNI CAGNNNCTG 2 cut(s) 262, 1759
Ama87I CYCGRG 2 cut(s) 1079, 1979
Aor13HI TCCGGA 1 cut(s) 2213
AoxI GGCC 5 cut(s) 98, 247, 1637, 1646, 1943
ApeKI GCWGC 3 cut(s) 262, 1615, 2116
ApoI RAATTY 6 cut(s) 393, 706, 1198, 1608, 1704, 2047
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 4 cut(s) 540, 1034, 1250, 1538
AspLEI GCGC 2 cut(s) 501, 2127
AspS9I GGNCC 7 cut(s) 160, 827, 1301, 1349, 1637, 1811, 2338
AsuHPI GGTGA 2 cut(s) 1127, 2126
AsuII TTCGAA 2 cut(s) 1261, 1281
AvaI CYCGRG 2 cut(s) 1079, 1979
AvaII GGWCC 6 cut(s) 160, 827, 1301, 1349, 1811, 2338
BalI TGGCCA 1 cut(s) 1648
BbsI GAAGAC 1 cut(s) 1270
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 3 cut(s) 249, 1627, 2103
BccI CCATC 6 cut(s) 452, 510, 1020, 1163, 1276, 1301
BciT130I CCWGG 4 cut(s) 93, 257, 719, 1627
BciVI GTATCC 1 cut(s) 2087
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 1982, 2330
BfaI CTAG 8 cut(s) 44, 399, 738, 791, 837, 950, 1367, 1889
BfmI CTRYAG 1 cut(s) 2162
BfuI GTATCC 1 cut(s) 2087
BglI GCCNNNNNGGC 1 cut(s) 1645
BglII AGATCT 3 cut(s) 151, 934, 1891
BisI GCNGC 3 cut(s) 263, 1616, 2117
BlsI GCNGC 3 cut(s) 264, 1617, 2118
BmcAI AGTACT 2 cut(s) 61, 1714
Bme1390I CCNGG 4 cut(s) 93, 257, 719, 1627
Bme18I GGWCC 6 cut(s) 160, 827, 1301, 1349, 1811, 2338
BmeT110I CYCGRG 2 cut(s) 1079, 1979
BmgT120I GGNCC 7 cut(s) 160, 827, 1301, 1349, 1637, 1811, 2338
BmiI GGNNCC 4 cut(s) 162, 1351, 1590, 1638
BmrFI CCNGG 4 cut(s) 93, 257, 719, 1627
BmrI ACTGGG 2 cut(s) 488, 2079
BmsI GCATC 6 cut(s) 188, 355, 617, 750, 993, 2120
BmuI ACTGGG 2 cut(s) 488, 2079
BpiI GAAGAC 1 cut(s) 1270
BpmI CTGGAG 2 cut(s) 475, 2312
Bpu14I TTCGAA 2 cut(s) 1261, 1281
BpuEI CTTGAG 3 cut(s) 140, 385, 604
BsaBI GATNNNNATC 1 cut(s) 1173
BsaJI CCNNGG 5 cut(s) 717, 764, 823, 1203, 2088
BsaWI WCCGGW 1 cut(s) 2213
Bsc4I CCNNNNNNNGG 6 cut(s) 256, 289, 646, 1255, 2016, 2335
Bse118I RCCGGY 1 cut(s) 245
Bse3DI GCAATG 4 cut(s) 48, 88, 1542, 1856
Bse8I GATNNNNATC 1 cut(s) 1173
BseAI TCCGGA 1 cut(s) 2213
BseBI CCWGG 4 cut(s) 93, 257, 719, 1627
BseDI CCNNGG 5 cut(s) 717, 764, 823, 1203, 2088
BseGI GGATG 7 cut(s) 252, 881, 1031, 1174, 1268, 1954, 2025
BseJI GATNNNNATC 1 cut(s) 1173
BseLI CCNNNNNNNGG 6 cut(s) 256, 289, 646, 1255, 2016, 2335
BseMI GCAATG 4 cut(s) 48, 88, 1542, 1856
BseMII CTCAG 3 cut(s) 349, 526, 2373
BseXI GCAGC 3 cut(s) 249, 1627, 2103
BsgI GTGCAG 3 cut(s) 248, 1515, 2102
BshFI GGCC 5 cut(s) 100, 249, 1639, 1648, 1945
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 2 cut(s) 1079, 1979
BsiSI CCGG 3 cut(s) 246, 1946, 2214
BslFI GGGAC 3 cut(s) 1221, 1335, 2063
BslI CCNNNNNNNGG 6 cut(s) 256, 289, 646, 1255, 2016, 2335
BsmAI GTCTC 2 cut(s) 1982, 2330
BsmBI CGTCTC 1 cut(s) 1982
BsmFI GGGAC 3 cut(s) 1221, 1335, 2063
BsnI GGCC 5 cut(s) 100, 249, 1639, 1648, 1945
BsoBI CYCGRG 2 cut(s) 1079, 1979
Bsp119I TTCGAA 2 cut(s) 1261, 1281
Bsp1286I GDGCHC 1 cut(s) 133
Bsp13I TCCGGA 1 cut(s) 2213
Bsp1407I TGTACA 2 cut(s) 1395, 1750
Bsp19I CCATGG 1 cut(s) 2088
BspACI CCGC 1 cut(s) 1660
BspANI GGCC 5 cut(s) 100, 249, 1639, 1648, 1945
BspCNI CTCAG 3 cut(s) 348, 525, 2372
BspEI TCCGGA 1 cut(s) 2213
BspHI TCATGA 1 cut(s) 2302
BspLI GGNNCC 4 cut(s) 162, 1351, 1590, 1638
BspMAI CTGCAG 1 cut(s) 2166
BspPI GGATC 2 cut(s) 896, 1924
BspT104I TTCGAA 2 cut(s) 1261, 1281
BsrDI GCAATG 4 cut(s) 48, 88, 1542, 1856
BsrFI RCCGGY 1 cut(s) 245
BsrGI TGTACA 2 cut(s) 1395, 1750
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 5 cut(s) 717, 764, 823, 1203, 2088
BssNAI GTATAC 1 cut(s) 1335
BssT1I CCWWGG 3 cut(s) 823, 1203, 2088
Bst1107I GTATAC 1 cut(s) 1335
Bst2UI CCWGG 4 cut(s) 93, 257, 719, 1627
Bst4CI ACNGT 5 cut(s) 209, 1258, 1755, 1825, 2171
Bst6I CTCTTC 1 cut(s) 307
BstAUI TGTACA 2 cut(s) 1395, 1750
BstBI TTCGAA 2 cut(s) 1261, 1281
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 9 cut(s) 335, 512, 577, 599, 806, 1384, 1685, 2195, 2359
BstDSI CCRYGG 1 cut(s) 2088
BstF5I GGATG 7 cut(s) 252, 881, 1031, 1174, 1268, 1954, 2025
BstHHI GCGC 2 cut(s) 501, 2127
BstMAI GTCTC 2 cut(s) 1982, 2330
BstMWI GCNNNNNNNGC 4 cut(s) 117, 1636, 1645, 2122
BstNI CCWGG 4 cut(s) 93, 257, 719, 1627
BstNSI RCATGY 2 cut(s) 637, 664
BstSCI CCNGG 4 cut(s) 91, 255, 717, 1625
BstSFI CTRYAG 1 cut(s) 2162
BstV1I GCAGC 3 cut(s) 249, 1627, 2103
BstV2I GAAGAC 1 cut(s) 1270
BstX2I RGATCY 4 cut(s) 151, 934, 1891, 1916
BstXI CCANNNNNNTGG 1 cut(s) 1850
BstYI RGATCY 4 cut(s) 151, 934, 1891, 1916
BstZ17I GTATAC 1 cut(s) 1335
BsuI GTATCC 1 cut(s) 2087
BsuRI GGCC 5 cut(s) 100, 249, 1639, 1648, 1945
BtgI CCRYGG 1 cut(s) 2088
BtgZI GCGATG 1 cut(s) 2097
BtsCI GGATG 7 cut(s) 252, 881, 1031, 1174, 1268, 1954, 2025
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 5 cut(s) 388, 551, 1503, 1848, 1995
Cac8I GCNNGC 1 cut(s) 177
CaiI CAGNNNCTG 2 cut(s) 262, 1759
CciI TCATGA 1 cut(s) 2302
CfoI GCGC 2 cut(s) 501, 2127
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 7 cut(s) 160, 827, 1301, 1349, 1637, 1811, 2338
Csp6I GTAC 9 cut(s) 60, 445, 1396, 1698, 1713, 1751, 2006, 2140, 2172
CviAII CATG 8 cut(s) 13, 241, 634, 661, 1141, 1748, 2089, 2303
CviQI GTAC 9 cut(s) 60, 445, 1396, 1698, 1713, 1751, 2006, 2140, 2172
DdeI CTNAG 9 cut(s) 335, 512, 577, 599, 806, 1384, 1685, 2195, 2359
DraI TTTAAA 2 cut(s) 342, 519
EaeI YGGCCR 2 cut(s) 247, 1646
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
Eco130I CCWWGG 3 cut(s) 823, 1203, 2088
Eco47I GGWCC 6 cut(s) 160, 827, 1301, 1349, 1811, 2338
Eco57I CTGAAG 2 cut(s) 527, 548
Eco88I CYCGRG 2 cut(s) 1079, 1979
EcoO109I RGGNCCY 3 cut(s) 160, 1349, 1637
EcoRII CCWGG 4 cut(s) 91, 255, 717, 1625
EcoT14I CCWWGG 3 cut(s) 823, 1203, 2088
ErhI CCWWGG 3 cut(s) 823, 1203, 2088
Esp3I CGTCTC 1 cut(s) 1982
FaeI CATG 8 cut(s) 16, 244, 637, 664, 1144, 1751, 2092, 2306
FaqI GGGAC 3 cut(s) 1221, 1335, 2063
FatI CATG 8 cut(s) 12, 240, 633, 660, 1140, 1747, 2088, 2302
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 1334
Fnu4HI GCNGC 3 cut(s) 263, 1616, 2117
FokI GGATG 7 cut(s) 239, 868, 1038, 1181, 1255, 1961, 2032
Fsp4HI GCNGC 3 cut(s) 263, 1616, 2117
FspBI CTAG 8 cut(s) 44, 399, 738, 791, 837, 950, 1367, 1889
FspI TGCGCA 1 cut(s) 2126
GlaI GCGC 2 cut(s) 500, 2126
GluI GCNGC 3 cut(s) 263, 1616, 2117
GsuI CTGGAG 2 cut(s) 475, 2312
HaeIII GGCC 5 cut(s) 100, 249, 1639, 1648, 1945
HapII CCGG 3 cut(s) 246, 1946, 2214
HhaI GCGC 2 cut(s) 501, 2127
Hin1II CATG 8 cut(s) 16, 244, 637, 664, 1144, 1751, 2092, 2306
Hin6I GCGC 2 cut(s) 499, 2125
HinP1I GCGC 2 cut(s) 499, 2125
HincII GTYRAC 2 cut(s) 1445, 2191
HindII GTYRAC 2 cut(s) 1445, 2191
HindIII AAGCTT 2 cut(s) 1381, 1687
HinfI GANTC 8 cut(s) 299, 907, 946, 953, 1082, 1622, 1762, 1792
HpaII CCGG 3 cut(s) 246, 1946, 2214
HphI GGTGA 2 cut(s) 1127, 2126
Hpy166II GTNNAC 3 cut(s) 1335, 1445, 2191
Hpy8I GTNNAC 3 cut(s) 1335, 1445, 2191
HpyCH4III ACNGT 5 cut(s) 209, 1258, 1755, 1825, 2171
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 1636, 1645, 2122
HpyF3I CTNAG 9 cut(s) 335, 512, 577, 599, 806, 1384, 1685, 2195, 2359
Hsp92II CATG 8 cut(s) 16, 244, 637, 664, 1144, 1751, 2092, 2306
HspAI GCGC 2 cut(s) 499, 2125
Kpn2I TCCGGA 1 cut(s) 2213
Ksp22I TGATCA 1 cut(s) 235
LmnI GCTCC 3 cut(s) 259, 642, 2384
Lsp1109I GCAGC 3 cut(s) 249, 1627, 2103
LweI GCATC 6 cut(s) 188, 355, 617, 750, 993, 2120
MaeI CTAG 8 cut(s) 44, 399, 738, 791, 837, 950, 1367, 1889
MaeIII GTNAC 3 cut(s) 368, 625, 1362
MboII GAAGA 8 cut(s) 206, 294, 668, 1151, 1270, 1275, 1924, 1964
MfeI CAATTG 1 cut(s) 1778
MflI RGATCY 4 cut(s) 151, 934, 1891, 1916
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 1648
MluNI TGGCCA 1 cut(s) 1648
MlyI GAGTC 3 cut(s) 293, 955, 1091
MmeI TCCRAC 4 cut(s) 197, 1161, 1482, 1997
Mox20I TGGCCA 1 cut(s) 1648
MroI TCCGGA 1 cut(s) 2213
MroXI GAANNNNTTC 4 cut(s) 540, 1034, 1250, 1538
MscI TGGCCA 1 cut(s) 1648
MslI CAYNNNNRTG 4 cut(s) 807, 1167, 1358, 1416
Msp20I TGGCCA 1 cut(s) 1648
MspA1I CMGCKG 1 cut(s) 1618
MspI CCGG 3 cut(s) 246, 1946, 2214
MspR9I CCNGG 4 cut(s) 93, 257, 719, 1627
MssI GTTTAAAC 1 cut(s) 519
MunI CAATTG 1 cut(s) 1778
MvaI CCWGG 4 cut(s) 93, 257, 719, 1627
MwoI GCNNNNNNNGC 4 cut(s) 117, 1636, 1645, 2122
NcoI CCATGG 1 cut(s) 2088
NlaIII CATG 8 cut(s) 16, 244, 637, 664, 1144, 1751, 2092, 2306
NlaIV GGNNCC 4 cut(s) 162, 1351, 1590, 1638
NmeAIII GCCGAG 1 cut(s) 789
NmuCI GTSAC 1 cut(s) 1362
NsbI TGCGCA 1 cut(s) 2126
NspI RCATGY 2 cut(s) 637, 664
NspV TTCGAA 2 cut(s) 1261, 1281
PaeR7I CTCGAG 1 cut(s) 1079
PagI TCATGA 1 cut(s) 2302
PciI ACATGT 1 cut(s) 660
PcsI WCGNNNNNNNCGW 1 cut(s) 1029
PdmI GAANNNNTTC 4 cut(s) 540, 1034, 1250, 1538
PfeI GAWTC 5 cut(s) 907, 953, 1622, 1762, 1792
PflMI CCANNNNNTGG 1 cut(s) 2335
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 3 cut(s) 264, 1617, 2118
PleI GAGTC 3 cut(s) 293, 954, 1090
PmeI GTTTAAAC 1 cut(s) 519
PpsI GAGTC 3 cut(s) 293, 954, 1090
PpuMI RGGWCCY 2 cut(s) 160, 1349
PscI ACATGT 1 cut(s) 660
PsiI TTATAA 1 cut(s) 1479
Psp5II RGGWCCY 2 cut(s) 160, 1349
Psp6I CCWGG 4 cut(s) 91, 255, 717, 1625
PspGI CCWGG 4 cut(s) 91, 255, 717, 1625
PspN4I GGNNCC 4 cut(s) 162, 1351, 1590, 1638
PspPI GGNCC 7 cut(s) 160, 827, 1301, 1349, 1637, 1811, 2338
PspPPI RGGWCCY 2 cut(s) 160, 1349
PstI CTGCAG 1 cut(s) 2166
PstNI CAGNNNCTG 2 cut(s) 262, 1759
PsuI RGATCY 4 cut(s) 151, 934, 1891, 1916
PvuII CAGCTG 1 cut(s) 1618
RsaI GTAC 9 cut(s) 61, 446, 1397, 1699, 1714, 1752, 2007, 2141, 2173
RsaNI GTAC 9 cut(s) 60, 445, 1396, 1698, 1713, 1751, 2006, 2140, 2172
RseI CAYNNNNRTG 4 cut(s) 807, 1167, 1358, 1416
SatI GCNGC 3 cut(s) 263, 1616, 2117
Sau96I GGNCC 7 cut(s) 160, 827, 1301, 1349, 1637, 1811, 2338
ScaI AGTACT 2 cut(s) 61, 1714
SchI GAGTC 3 cut(s) 293, 955, 1091
ScrFI CCNGG 4 cut(s) 93, 257, 719, 1627
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 6 cut(s) 188, 355, 617, 750, 993, 2120
SfcI CTRYAG 1 cut(s) 2162
SfiI GGCCNNNNNGGCC 1 cut(s) 1645
Sfr274I CTCGAG 1 cut(s) 1079
SfuI TTCGAA 2 cut(s) 1261, 1281
SinI GGWCC 6 cut(s) 160, 827, 1301, 1349, 1811, 2338
SlaI CTCGAG 1 cut(s) 1079
SmiMI CAYNNNNRTG 4 cut(s) 807, 1167, 1358, 1416
SmlI CTYRAG 4 cut(s) 155, 364, 583, 1079
SmoI CTYRAG 4 cut(s) 155, 364, 583, 1079
SsiI CCGC 1 cut(s) 1660
SspMI CTAG 8 cut(s) 44, 399, 738, 791, 837, 950, 1367, 1889
StyD4I CCNGG 4 cut(s) 91, 255, 717, 1625
StyI CCWWGG 3 cut(s) 823, 1203, 2088
TaaI ACNGT 5 cut(s) 209, 1258, 1755, 1825, 2171
TaqI TCGA 6 cut(s) 297, 1080, 1124, 1261, 1281, 1579
TatI WGTACW 4 cut(s) 59, 1395, 1712, 1750
TfiI GAWTC 5 cut(s) 907, 953, 1622, 1762, 1792
TscAI CASTG 5 cut(s) 388, 551, 1503, 1855, 2002
TseFI GTSAC 1 cut(s) 1362
TseI GCWGC 3 cut(s) 262, 1615, 2116
Tsp45I GTSAC 1 cut(s) 1362
TspDTI ATGAA 7 cut(s) 668, 791, 975, 1531, 1893, 1965, 2319
TspGWI ACGGA 1 cut(s) 2157
TspRI CASTG 5 cut(s) 388, 551, 1503, 1855, 2002
Van91I CCANNNNNTGG 1 cut(s) 2335
VpaK11BI GGWCC 6 cut(s) 160, 827, 1301, 1349, 1811, 2338
XapI RAATTY 6 cut(s) 393, 706, 1198, 1608, 1704, 2047
XbaI TCTAGA 2 cut(s) 737, 949
XceI RCATGY 2 cut(s) 637, 664
XhoI CTCGAG 1 cut(s) 1079
XmiI GTMKAC 1 cut(s) 1334
XmnI GAANNNNTTC 4 cut(s) 540, 1034, 1250, 1538
XspI CTAG 8 cut(s) 44, 399, 738, 791, 837, 950, 1367, 1889
ZrmI AGTACT 2 cut(s) 61, 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.