MD08G1239100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000281
Physical Location & Seq
Forward (+)
30590344 .. 30592596
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1239100.v1.1.491

Sequence Viewer

Length: 2253 bp
ATGGATAGGCCTTTGAGACTTGTTTTACTTGTGAGTTTCCTAAGCATTGCAGCCACTCCCATTAGTTTGGGCAATGCAAATCTGGGACTTCGGGTTCCTTGCACTGAAAATGAGAGGCAAGCCCTTCTGATGCTCAAGCAAGATTTTCTTGATTCTTCGAACCGGATTTCATCCTGGATTGGCGAAGGAGATTGTTGCTTTTGGCCTGGAGTTGTCTGTGGTAACTTAACCGGCCATGTCCGCGAGCTCCACCTTGGTAATTACAATTTGGGTGGTAAGCTGAATCCTTCTCTGCTTAATTTAAGCAATCTCGCTTACTTGGACCTAAGCAACAATGAATTTGAAGGAATGCAGATTCCTAACTTCTTCGGTTCTCTTACAAGTTTAAGGCATCTTGACCTCTCACAATCAGATTTTCAGGGAATAATTCATCACCAGCTGGGGAATCTTTCTAATCTACGCTTTCTTGACCTCCATGGTAACTATTTTGAGGTTAAGAGCCTGCAATGGATTTCTGGTCTTTCTCAGCTGCAGCACCTGGACATGAGTGGTGTAGATCTCAGAGAAGCATCCGATTTGTTCCAGGTGACAAACACTTTCCCCTCTTTGCTAGAGTACTTGAATATGTCTGATTGCGGACTTTATCAAATACCATTTGGAATTGCAAATGCTTCTGCTCTTAAAGTTCTTAATCTCGAGGGGAATTCCATCAGTTCTATTATACCAAAATGGTTGTACAGCCTTAGCCATCTCGAGTCATTATTCCTTTCCTACAATCACTTGCGTGGCCAAATCTCAAACTCCATTGCAAACTTAACAGACATTGTCAATCTTGACTTATTTCATAATCAACTTGAAGAGGAACTCCCAACCTCAATGGAAAAACTTTGCAAGTTGATGGTTTTTGATCTGTCGAGGAACCGTGTCAATGGGAGGGTATCCGAAATCTTTGAAAGTTTGTCTAGGTGCAATTCAAGTCAACTAGAGTCCTTGAGCTTATCCAATAATAATCTTTCTGGTCTTTTAACAGATGAGCTAGGAAATTTTGAAAAGTTACGTTTCCTTGATCTTTCAAGCAATTCAATATCAGGTTCCATTCCAATGTCCTTAGGAAATCTGTCATGCTTAGAAGAATTACTCATTCATAATAATTCGTTCAAGGGTGTCGTCTCTGAGGTTCATTTTACCAATCTCACAAAATTGGTGAAACTTTATGCAAAAAATAATTCATTGACTCTAGAAACCAGTACAGATTGGCATCCACCATTTCAACTCCGAGTGTTGTTCTTAGATTCTTGGTATCTAGGGCCAGAGTTACCTTTGTGGTTTCATAGACAAACACAATTGCAGTATCTAAGCATGGTCAACACAAGAATTTCAGGTACCATCCCAACCTGGTTTTGGAACTTTTCTTCCCAACTAAGATTTGTCGATCTCTCTCACAATCAATTGTATGGGGCAGTTCCAAATATAGTTGGTGCTCCTTTAGACATAATTGACCTAAGTTCAAACCAATTCAATGGTCCGTTACCTCTCATCTCCTCCACCGTTGATGTACTAGATCTTTCCAATTCAGCATTTTCCGGATCCGTCTTTCACTTCTTTTGTGATAGCATAGATGGGCCAAAACAGCTCAGAATTCTTTATCTTGAGAACAACTTTCTTGATGGACCAATTCCTGATTGTTGGAAAAACTGGAAAAAGTTGATTGTCCTAAATTTAGACAACAACAATTTGACAGGGAACATTCCAAGCTCCATAGGATACTTACTTTTCCTCGAATCGCTGCACTTGCGCAATAATCACCTGTTTGGAGAGTTGCCAATGTCCCTACAAAATTGTCAGAACCTCTTAGTTGCTGACCTTGGTGAAAATAAGTTTGTTGGAAGCATACCAATGTGGATAGGAAAAAGTCTTTCAAATTTGATCGTTCTCATCCTTCGTTCCAATAAGCTTCAGAGTGACATTCCTAAGGAAATCTGCAGTCTTGTCAATCTCCAAATCTTGGACCTTGCGTATAATAATCTCTCCGGAACAATCCCAAAGTGTTTCAACAATTTCGGCGCCATGGCCACCTTGTCAAACTCAGGAGGTCCCATTTCATTCTTCAGTTATATGTACGGTTCGCCTGAGCAGTACATGGAGGAAGCAATCTTGGTGAGAAAAGGTAGAACAGACAGATATAACAAATTTCTTAGCTTGGTAGCTAACTTGGACCTTTCACACAACATGATATCTGGAGAGATTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

751

Amino Acids

84.05

Weight (kDa)

5.4

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 73 4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 184 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 128 - 184 6.1e-08 Leucine rich repeat
LRR_14 PF23598 171 - 336 1.7e-08 Leucine-rich repeat region
LRR_8 PF13855 226 - 285 1.1e-09 Leucine rich repeat
LRR_14 PF23598 336 - 409 4.8e-06 Leucine-rich repeat region
LRR_8 PF13855 544 - 603 4.5e-06 Leucine rich repeat
LRR_8 PF13855 622 - 676 8.4e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21840 FvH4_5g27300 FvH4_7g26560 FvH4_7g26561 FvH4_7g26564 FvH4_7g26565 FvH4_7g26565 FvH4_7g26566 FvH4_7g26567 FvH4_7g26600 FvH4_7g26610
malus_domestica MD01G1000900.v1.1 MD01G1131400.v1.1 MD01G1165400.v1.1 MD01G1165600.v1.1 MD01G1172100.v1.1 MD01G1172300.v1.1 MD01G1172800.v1.1 MD01G1173000.v1.1 MD01G1174800.v1.1 MD01G1178700.v1.1 MD01G1178900.v1.1 MD01G1179300.v1.1 MD01G1179700.v1.1 MD01G1179800.v1.1 MD01G1180500.v1.1 MD04G1020400.v1.1 MD06G1026600.v1.1 MD08G1144300.v1.1 MD08G1144400.v1.1 MD08G1237900.v1.1 MD08G1238300.v1.1 MD08G1238900.v1.1 MD08G1239000.v1.1 MD08G1239100.v1.1 MD08G1239200.v1.1 MD11G1217100.v1.1 MD15G1414600.v1.1 MD15G1414700.v1.1 MD15G1414900.v1.1 MD15G1415000.v1.1 MD15G1426700.v1.1 MD15G1427000.v1.1 MD15G1429700.v1.1 MD15G1429800.v1.1 MD15G1430200.v1.1 MD15G1430300.v1.1 MD15G1439600.v1.1
prunus_persica Prupe.1G055200_v2.0.a1 Prupe.1G526400_v2.0.a1 Prupe.1G541000_v2.0.a1 Prupe.1G575100_v2.0.a1 Prupe.1G575200_v2.0.a1 Prupe.1G575300_v2.0.a1 Prupe.1G575500_v2.0.a1 Prupe.2G270000_v2.0.a1 Prupe.2G270200_v2.0.a1 Prupe.3G019500_v2.0.a1 Prupe.3G019600_v2.0.a1 Prupe.3G021800_v2.0.a1 Prupe.3G029200_v2.0.a1 Prupe.3G055300_v2.0.a1 Prupe.3G064900_v2.0.a1 Prupe.6G311600_v2.0.a1
pyrus_communis pycom01g18380 pycom01g18440 pycom01g18460 pycom01g18480 pycom01g18930 pycom01g18940 pycom01g18960 pycom01g18990 pycom01g19030 pycom01g19050 pycom01g19070 pycom08g20740 pycom08g20750 pycom11g19100 pycom12g20310 pycom14g10700 pycom15g10840 pycom15g37770 pycom15g37830 pycom15g37860 pycom15g38010 pycom15g38040 pycom15g38750 pycom420g00350 pycom420g00400 pycom420g00410 pycom420g00510 pycom420g00540 pycom976g00210
rosa_chinensis RchiOBHm_Chr1g0373231 RchiOBHm_Chr1g0373241 RchiOBHm_Chr1g0373251
rosa_laevigata RLG00000026834 RLG00000026835 RLG00000026836 RLG00000026837 RLG00000026839 RLG00000026840 RLG00000026841
rosa_multiflora Rmu_co8123616.1_g000001 Rmu_sc0000170.1_g000006 Rmu_sc0000170.1_g000007 Rmu_sc0000170.1_g000009 Rmu_sc0008079.1_g000015 Rmu_sc0013594.1_g000002
rosa_roxburghii Rroxscaffold_4G00284310 Rroxscaffold_4G00284320 Rroxscaffold_4G00284350 Rroxscaffold_4G00284370
rosa_rugosa Rorug01G0376700 Rorug01G0376800
rosa_samantha Rh1BG349500 Rh1CG362800 Rh3BG125200
rosa_wichuraiana Rw0G003040 Rw0G015420 Rw1G034010 Rw1G034020 Rw1G034030 Rw1G034950 Rw1G034960 Rw1G034970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1796
Acc65I GGTACC 1 cut(s) 1382
AccB1I GGYRCC 2 cut(s) 1382, 2063
AccB7I CCANNNNNTGG 1 cut(s) 2005
AccII CGCG 1 cut(s) 243
AccIII TCCGGA 2 cut(s) 1583, 2030
AciI CCGC 2 cut(s) 241, 636
AclWI GGATC 2 cut(s) 1581, 1594
AcoI YGGCCR 3 cut(s) 232, 787, 2070
AcsI RAATTY 8 cut(s) 338, 703, 1042, 1374, 1638, 1717, 1921, 2189
AcuI CTGAAG 2 cut(s) 1940, 2092
AcyI GRCGYC 1 cut(s) 2064
AfaI GTAC 7 cut(s) 617, 737, 1249, 1384, 1557, 2120, 2138
AfiI CCNNNNNNNGG 2 cut(s) 1401, 2005
AjnI CCWGG 5 cut(s) 173, 205, 537, 582, 1394
AleI CACNNNNGTG 1 cut(s) 783
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 1397, 1429
Alw21I GWGCWC 2 cut(s) 249, 1483
Alw26I GTCTC 2 cut(s) 10, 1174
AlwI GGATC 2 cut(s) 1581, 1594
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 2 cut(s) 695, 752
Aor13HI TCCGGA 2 cut(s) 1583, 2030
AoxI GGCC 7 cut(s) 8, 203, 232, 787, 1307, 1622, 2070
ApeKI GCWGC 4 cut(s) 50, 529, 532, 1786
ApoI RAATTY 8 cut(s) 338, 703, 1042, 1374, 1638, 1717, 1921, 2189
Asp718I GGTACC 1 cut(s) 1382
AspLEI GCGC 2 cut(s) 1797, 2066
AspS9I GGNCC 8 cut(s) 322, 1307, 1523, 1622, 1670, 2008, 2093, 2215
AsuHPI GGTGA 6 cut(s) 425, 598, 1216, 1796, 1880, 2170
AsuII TTCGAA 1 cut(s) 158
AvaI CYCGRG 2 cut(s) 695, 752
AvaII GGWCC 6 cut(s) 322, 1523, 1670, 2008, 2093, 2215
AxyI CCTNAGG 2 cut(s) 1108, 1971
BalI TGGCCA 2 cut(s) 789, 2072
BamHI GGATCC 1 cut(s) 1586
BanI GGYRCC 2 cut(s) 1382, 2063
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 249, 1483
BbvI GCAGC 4 cut(s) 62, 516, 544, 1773
BccI CCATC 6 cut(s) 716, 756, 892, 1394, 1613, 1661
BciT130I CCWGG 5 cut(s) 175, 207, 539, 584, 1396
BciVI GTATCC 2 cut(s) 949, 1757
BcoDI GTCTC 2 cut(s) 10, 1174
BfaI CTAG 7 cut(s) 611, 963, 983, 1037, 1238, 1304, 1559
BfmI CTRYAG 2 cut(s) 530, 1981
BfoI RGCGCY 1 cut(s) 2067
BfuI GTATCC 2 cut(s) 949, 1757
BglII AGATCT 2 cut(s) 556, 1561
BisI GCNGC 4 cut(s) 51, 530, 533, 1787
BlsI GCNGC 4 cut(s) 52, 531, 534, 1788
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 5 cut(s) 175, 207, 539, 584, 1396
Bme18I GGWCC 6 cut(s) 322, 1523, 1670, 2008, 2093, 2215
BmeT110I CYCGRG 2 cut(s) 695, 752
BmgT120I GGNCC 8 cut(s) 322, 1307, 1523, 1622, 1670, 2008, 2093, 2215
BmiI GGNNCC 7 cut(s) 96, 920, 1093, 1384, 1588, 2065, 2095
BmrFI CCNGG 5 cut(s) 175, 207, 539, 584, 1396
BmsI GCATC 4 cut(s) 120, 400, 578, 1267
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 4 cut(s) 41, 326, 743, 2130
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 3 cut(s) 119, 1012, 1670
BsaBI GATNNNNATC 1 cut(s) 1257
BsaHI GRCGYC 1 cut(s) 2064
BsaJI CCNNGG 4 cut(s) 253, 475, 1864, 2067
BsaWI WCCGGW 3 cut(s) 162, 1583, 2030
Bsc4I CCNNNNNNNGG 2 cut(s) 1401, 2005
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 1245, 1700
Bse21I CCTNAGG 2 cut(s) 1108, 1971
Bse3DI GCAATG 4 cut(s) 45, 79, 512, 804
Bse8I GATNNNNATC 1 cut(s) 1257
BseAI TCCGGA 2 cut(s) 1583, 2030
BseBI CCWGG 5 cut(s) 175, 207, 539, 584, 1396
BseDI CCNNGG 4 cut(s) 253, 475, 1864, 2067
BseGI GGATG 5 cut(s) 170, 569, 1258, 1386, 1935
BseJI GATNNNNATC 1 cut(s) 1257
BseLI CCNNNNNNNGG 2 cut(s) 1401, 2005
BseMI GCAATG 4 cut(s) 45, 79, 512, 804
BseMII CTCAG 6 cut(s) 539, 574, 1164, 1648, 2100, 2121
BseNI ACTGG 2 cut(s) 1245, 1700
BseRI GAGGAG 1 cut(s) 1531
BseXI GCAGC 4 cut(s) 62, 516, 544, 1773
BseYI CCCAGC 1 cut(s) 439
BsgI GTGCAG 1 cut(s) 1772
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 7 cut(s) 10, 205, 234, 789, 1309, 1624, 2072
BshNI GGYRCC 2 cut(s) 1382, 2063
BsiHKAI GWGCWC 2 cut(s) 249, 1483
BsiHKCI CYCGRG 2 cut(s) 695, 752
BsiSI CCGG 4 cut(s) 163, 231, 1584, 2031
BslFI GGGAC 3 cut(s) 99, 1813, 2079
BslI CCNNNNNNNGG 2 cut(s) 1401, 2005
BsmAI GTCTC 2 cut(s) 10, 1174
BsmBI CGTCTC 1 cut(s) 1174
BsmFI GGGAC 3 cut(s) 99, 1813, 2079
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 7 cut(s) 10, 205, 234, 789, 1309, 1624, 2072
BsoBI CYCGRG 2 cut(s) 695, 752
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 249, 1483
Bsp13I TCCGGA 2 cut(s) 1583, 2030
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
Bsp19I CCATGG 2 cut(s) 475, 2067
BspACI CCGC 2 cut(s) 241, 636
BspANI GGCC 7 cut(s) 10, 205, 234, 789, 1309, 1624, 2072
BspCNI CTCAG 6 cut(s) 538, 573, 1165, 1647, 2099, 2122
BspEI TCCGGA 2 cut(s) 1583, 2030
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 7 cut(s) 96, 920, 1093, 1384, 1588, 2065, 2095
BspMAI CTGCAG 2 cut(s) 534, 1985
BspPI GGATC 2 cut(s) 1581, 1594
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 2 cut(s) 1382, 2063
BsrDI GCAATG 4 cut(s) 45, 79, 512, 804
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 2 cut(s) 1245, 1700
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 4 cut(s) 253, 475, 1864, 2067
BssMI GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
BssNI GRCGYC 1 cut(s) 2064
BssT1I CCWWGG 4 cut(s) 253, 475, 1864, 2067
Bst2UI CCWGG 5 cut(s) 175, 207, 539, 584, 1396
Bst4CI ACNGT 3 cut(s) 923, 1549, 2123
Bst6I CTCTTC 1 cut(s) 852
BstACI GRCGYC 1 cut(s) 2064
BstAUI TGTACA 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 120, 245, 503
BstDSI CCRYGG 2 cut(s) 475, 2067
BstF5I GGATG 5 cut(s) 170, 569, 1258, 1386, 1935
BstFNI CGCG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 2067
BstHHI GCGC 2 cut(s) 1797, 2066
BstKTI GATC 7 cut(s) 559, 910, 1069, 1435, 1564, 1589, 1929
BstMAI GTCTC 2 cut(s) 10, 1174
BstMBI GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
BstMWI GCNNNNNNNGC 3 cut(s) 240, 1630, 1792
BstNI CCWGG 5 cut(s) 175, 207, 539, 584, 1396
BstSCI CCNGG 5 cut(s) 173, 205, 537, 582, 1394
BstSFI CTRYAG 2 cut(s) 530, 1981
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 4 cut(s) 62, 516, 544, 1773
BstX2I RGATCY 3 cut(s) 556, 1561, 1586
BstXI CCANNNNNNTGG 2 cut(s) 67, 1520
BstYI RGATCY 3 cut(s) 556, 1561, 1586
Bsu36I CCTNAGG 2 cut(s) 1108, 1971
BsuI GTATCC 2 cut(s) 949, 1757
BsuRI GGCC 7 cut(s) 10, 205, 234, 789, 1309, 1624, 2072
BtgI CCRYGG 2 cut(s) 475, 2067
BtsCI GGATG 5 cut(s) 170, 569, 1258, 1386, 1935
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 3 cut(s) 120, 245, 503
CaiI CAGNNNCTG 1 cut(s) 538
CfoI GCGC 2 cut(s) 1797, 2066
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 8 cut(s) 322, 1307, 1523, 1622, 1670, 2008, 2093, 2215
CsiI ACCWGGT 1 cut(s) 1394
Csp6I GTAC 7 cut(s) 616, 736, 1248, 1383, 1556, 2119, 2137
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 8 cut(s) 236, 476, 544, 1122, 1360, 2068, 2140, 2230
CviQI GTAC 7 cut(s) 616, 736, 1248, 1383, 1556, 2119, 2137
DinI GGCGCC 1 cut(s) 2065
DpnI GATC 7 cut(s) 558, 909, 1068, 1434, 1563, 1588, 1928
DpnII GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
EaeI YGGCCR 3 cut(s) 232, 787, 2070
Eam1104I CTCTTC 1 cut(s) 852
EarI CTCTTC 1 cut(s) 852
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 4 cut(s) 253, 475, 1864, 2067
Eco147I AGGCCT 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 2235
Eco47I GGWCC 6 cut(s) 322, 1523, 1670, 2008, 2093, 2215
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 1940, 2092
Eco81I CCTNAGG 2 cut(s) 1108, 1971
Eco88I CYCGRG 2 cut(s) 695, 752
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 2093
EcoRI GAATTC 2 cut(s) 703, 1638
EcoRII CCWGG 5 cut(s) 173, 205, 537, 582, 1394
EcoRV GATATC 1 cut(s) 2235
EcoT14I CCWWGG 4 cut(s) 253, 475, 1864, 2067
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 2065
EheI GGCGCC 1 cut(s) 2065
ErhI CCWWGG 4 cut(s) 253, 475, 1864, 2067
Esp3I CGTCTC 1 cut(s) 1174
FaeI CATG 8 cut(s) 239, 479, 547, 1125, 1363, 2071, 2143, 2233
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 3 cut(s) 99, 1813, 2079
FatI CATG 8 cut(s) 235, 475, 543, 1121, 1359, 2067, 2139, 2229
Fnu4HI GCNGC 4 cut(s) 51, 530, 533, 1787
FokI GGATG 5 cut(s) 157, 556, 1245, 1373, 1922
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 51, 530, 533, 1787
FspBI CTAG 7 cut(s) 611, 963, 983, 1037, 1238, 1304, 1559
FspI TGCGCA 1 cut(s) 1796
GlaI GCGC 2 cut(s) 1796, 2065
GluI GCNGC 4 cut(s) 51, 530, 533, 1787
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 2067
HaeIII GGCC 7 cut(s) 10, 205, 234, 789, 1309, 1624, 2072
HapII CCGG 4 cut(s) 163, 231, 1584, 2031
HhaI GCGC 2 cut(s) 1797, 2066
Hin1I GRCGYC 1 cut(s) 2064
Hin1II CATG 8 cut(s) 239, 479, 547, 1125, 1363, 2071, 2143, 2233
Hin6I GCGC 2 cut(s) 1795, 2064
HinP1I GCGC 2 cut(s) 1795, 2064
HincII GTYRAC 2 cut(s) 980, 1366
HindII GTYRAC 2 cut(s) 980, 1366
HindIII AAGCTT 1 cut(s) 1952
HpaII CCGG 4 cut(s) 163, 231, 1584, 2031
HphI GGTGA 6 cut(s) 425, 598, 1216, 1796, 1880, 2170
Hpy166II GTNNAC 2 cut(s) 980, 1366
Hpy8I GTNNAC 2 cut(s) 980, 1366
HpyAV CCTTC 5 cut(s) 134, 179, 297, 338, 1949
HpyCH4III ACNGT 3 cut(s) 923, 1549, 2123
HpyCH4IV ACGT 1 cut(s) 1057
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 1630, 1792
HpySE526I ACGT 1 cut(s) 1057
Hsp92I GRCGYC 1 cut(s) 2064
Hsp92II CATG 8 cut(s) 239, 479, 547, 1125, 1363, 2071, 2143, 2233
HspAI GCGC 2 cut(s) 1795, 2064
KasI GGCGCC 1 cut(s) 2063
Kpn2I TCCGGA 2 cut(s) 1583, 2030
KpnI GGTACC 1 cut(s) 1386
Kzo9I GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
LmnI GCTCC 3 cut(s) 252, 1486, 1760
Lsp1109I GCAGC 4 cut(s) 62, 516, 544, 1773
LweI GCATC 4 cut(s) 120, 400, 578, 1267
MabI ACCWGGT 1 cut(s) 1394
MaeI CTAG 7 cut(s) 611, 963, 983, 1037, 1238, 1304, 1559
MaeII ACGT 1 cut(s) 1057
MaeIII GTNAC 7 cut(s) 221, 479, 586, 1053, 1314, 1527, 1961
MalI GATC 7 cut(s) 558, 909, 1068, 1434, 1563, 1588, 1928
MboI GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
MboII GAAGA 6 cut(s) 147, 358, 869, 1142, 1404, 2098
MfeI CAATTG 2 cut(s) 1343, 1448
MflI RGATCY 3 cut(s) 556, 1561, 1586
MhlI GDGCHC 2 cut(s) 249, 1483
MlsI TGGCCA 2 cut(s) 789, 2072
MluNI TGGCCA 2 cut(s) 789, 2072
Mly113I GGCGCC 1 cut(s) 2064
MlyI GAGTC 3 cut(s) 764, 995, 1228
MmeI TCCRAC 2 cut(s) 1667, 1864
Mox20I TGGCCA 2 cut(s) 789, 2072
MroI TCCGGA 2 cut(s) 1583, 2030
MscI TGGCCA 2 cut(s) 789, 2072
MseI TTAA 9 cut(s) 227, 297, 302, 386, 495, 681, 690, 815, 1025
MslI CAYNNNNRTG 3 cut(s) 783, 1100, 1895
Msp20I TGGCCA 2 cut(s) 789, 2072
MspA1I CMGCKG 2 cut(s) 439, 529
MspI CCGG 4 cut(s) 163, 231, 1584, 2031
MspR9I CCNGG 5 cut(s) 175, 207, 539, 584, 1396
MunI CAATTG 2 cut(s) 1343, 1448
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 5 cut(s) 175, 207, 539, 584, 1396
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 240, 1630, 1792
NarI GGCGCC 1 cut(s) 2064
NcoI CCATGG 2 cut(s) 475, 2067
NdeII GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
NlaIII CATG 8 cut(s) 239, 479, 547, 1125, 1363, 2071, 2143, 2233
NlaIV GGNNCC 7 cut(s) 96, 920, 1093, 1384, 1588, 2065, 2095
NmuCI GTSAC 2 cut(s) 586, 1961
NsbI TGCGCA 1 cut(s) 1796
NspV TTCGAA 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 783
PaeR7I CTCGAG 2 cut(s) 695, 752
PceI AGGCCT 1 cut(s) 10
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 7 cut(s) 152, 283, 355, 445, 1292, 1781, 2245
PflFI GACNNNGTC 1 cut(s) 824
PflMI CCANNNNNTGG 1 cut(s) 2005
PfoI TCCNGGA 1 cut(s) 173
PkrI GCNGC 4 cut(s) 52, 531, 534, 1788
PleI GAGTC 3 cut(s) 763, 994, 1228
PluTI GGCGCC 1 cut(s) 2067
PpsI GAGTC 3 cut(s) 763, 994, 1228
PpuMI RGGWCCY 1 cut(s) 2093
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 2093
Psp6I CCWGG 5 cut(s) 173, 205, 537, 582, 1394
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 5 cut(s) 173, 205, 537, 582, 1394
PspN4I GGNNCC 7 cut(s) 96, 920, 1093, 1384, 1588, 2065, 2095
PspPI GGNCC 8 cut(s) 322, 1307, 1523, 1622, 1670, 2008, 2093, 2215
PspPPI RGGWCCY 1 cut(s) 2093
PstI CTGCAG 2 cut(s) 534, 1985
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 3 cut(s) 556, 1561, 1586
PsyI GACNNNGTC 1 cut(s) 824
PvuII CAGCTG 2 cut(s) 439, 529
RsaI GTAC 7 cut(s) 617, 737, 1249, 1384, 1557, 2120, 2138
RsaNI GTAC 7 cut(s) 616, 736, 1248, 1383, 1556, 2119, 2137
RseI CAYNNNNRTG 3 cut(s) 783, 1100, 1895
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 9 cut(s) 227, 297, 302, 386, 495, 681, 690, 815, 1025
SatI GCNGC 4 cut(s) 51, 530, 533, 1787
Sau3AI GATC 7 cut(s) 556, 907, 1066, 1432, 1561, 1586, 1926
Sau96I GGNCC 8 cut(s) 322, 1307, 1523, 1622, 1670, 2008, 2093, 2215
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 3 cut(s) 764, 995, 1228
ScrFI CCNGG 5 cut(s) 175, 207, 539, 584, 1396
SduI GDGCHC 2 cut(s) 249, 1483
SexAI ACCWGGT 1 cut(s) 1394
SfaNI GCATC 4 cut(s) 120, 400, 578, 1267
SfcI CTRYAG 2 cut(s) 530, 1981
SfoI GGCGCC 1 cut(s) 2065
Sfr274I CTCGAG 2 cut(s) 695, 752
SfuI TTCGAA 1 cut(s) 158
SinI GGWCC 6 cut(s) 322, 1523, 1670, 2008, 2093, 2215
SlaI CTCGAG 2 cut(s) 695, 752
SmiMI CAYNNNNRTG 3 cut(s) 783, 1100, 1895
SmlI CTYRAG 5 cut(s) 134, 695, 752, 991, 1649
SmoI CTYRAG 5 cut(s) 134, 695, 752, 991, 1649
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 2 cut(s) 241, 636
SspDI GGCGCC 1 cut(s) 2063
SspMI CTAG 7 cut(s) 611, 963, 983, 1037, 1238, 1304, 1559
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 5 cut(s) 173, 205, 537, 582, 1394
StyI CCWWGG 4 cut(s) 253, 475, 1864, 2067
TaaI ACNGT 3 cut(s) 923, 1549, 2123
TaiI ACGT 1 cut(s) 1060
TaqI TCGA 6 cut(s) 158, 696, 753, 914, 1431, 1779
TatI WGTACW 5 cut(s) 615, 735, 1247, 1555, 2136
TfiI GAWTC 7 cut(s) 152, 283, 355, 445, 1292, 1781, 2245
Tru1I TTAA 9 cut(s) 227, 297, 302, 386, 495, 681, 690, 815, 1025
Tru9I TTAA 9 cut(s) 227, 297, 302, 386, 495, 681, 690, 815, 1025
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 2 cut(s) 586, 1961
TseI GCWGC 4 cut(s) 50, 529, 532, 1786
Tsp45I GTSAC 2 cut(s) 586, 1961
TspDTI ATGAA 9 cut(s) 159, 351, 419, 833, 1133, 1169, 1218, 1319, 2091
TspGWI ACGGA 2 cut(s) 1515, 1579
TspRI CASTG 1 cut(s) 109
Tth111I GACNNNGTC 1 cut(s) 824
Van91I CCANNNNNTGG 1 cut(s) 2005
VpaK11BI GGWCC 6 cut(s) 322, 1523, 1670, 2008, 2093, 2215
XapI RAATTY 8 cut(s) 338, 703, 1042, 1374, 1638, 1717, 1921, 2189
XbaI TCTAGA 1 cut(s) 1237
XcmI CCANNNNNNNNNTGG 1 cut(s) 1398
XhoI CTCGAG 2 cut(s) 695, 752
XspI CTAG 7 cut(s) 611, 963, 983, 1037, 1238, 1304, 1559
ZrmI AGTACT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.