Rh1CG362900

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0012420
Physical Location & Seq
Forward (+)
63577415 .. 63581200
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG362900.1

Sequence Viewer

Length: 906 bp
ATGTACGCCTTTGTAATGCGTCCTGAGAGCTTGCCTAAATCTTATCATGAATTTATCCAGAAGACTGGTCCTGTTGCTCAGCCTGTATACAAGGCCGTAAGGGAGTGTTGTAGAGGATACCGAGTGGATATTGCCTCACTGGCTGATTACTTGGCTGAGAAAGGGAGAAGCGATATAAAATTGGAAGAGTTTCCATCCATTTTGCCTTGTTCTGTTATTCATCCCCACACAAATTCTTGTTTAGTGCACAACGCAACTGCAACATCAGCTACATTTAGGAAAACATTTCCGCTTTACTTCTCTTTGACGTTTGTACCATTTGTCGTTCTTCGCCTACAGAAGTTCCTGGCGGCCCCTGCTCGCACCTTTATGTTTGCTGTTAAAGATTCTGTTCGCTCAACAGCATTCTTGTCTGCTTTTGTTGGAATCTTTCAGGGTGTGATATGTTTGCACAGAAAAGTTGCATCAAAAGACAACAAGCTTGTATATTGGATTGCTGGTGGTCTATCTGCTCTTTCAGTTCTACTGGAGAAAAAGGCTAGGCGTGGTGAACTTGCTCTATATGTTCTTCCACGGGCAGGAGATTCTTTGTGGTATATTTTAGTAAACCGTCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTCTTGTTCTGTTTGTGTATGGGAGGAATTATGTATTACTTGGAACATGAGCCAGGCACTATGGCTCCATTCCTGAGGGGTCTGATTCGCCGCTTTCTTGCTAGCCGAATTAGTAATCCAGTCAATTCGTCTAATAGGAATGCTTCCTACACATATTTGCAAACTCTTGATGCCATGAAGGAGCCAAAGCTATTAGAGAATGATGGTTCTGAAGCACCTTCTGAGAAGAAATACAATCTTGAATCAATACCGGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

301

Amino Acids

33.88

Weight (kDa)

9.37

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM135_C_rich PF15982 72 - 200 4.8e-09 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 624
Acc36I ACCTGC 1 cut(s) 624
AccI GTMKAC 1 cut(s) 87
AciI CCGC 3 cut(s) 290, 350, 736
AcsI RAATTY 2 cut(s) 50, 232
AcuI CTGAAG 1 cut(s) 876
AfaI GTAC 2 cut(s) 5, 315
AfiI CCNNNNNNNGG 1 cut(s) 578
AgsI TTSAA 1 cut(s) 887
AjnI CCWGG 2 cut(s) 345, 697
AluBI AGCT 4 cut(s) 30, 269, 481, 835
AluI AGCT 4 cut(s) 30, 269, 481, 835
Alw21I GWGCWC 1 cut(s) 249
Alw44I GTGCAC 1 cut(s) 245
AoxI GGCC 2 cut(s) 93, 351
ApaLI GTGCAC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 50, 232
Asp700I GAANNNNTTC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 68, 352
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 2 cut(s) 560, 605
AsuNHI GCTAGC 1 cut(s) 746
AvaII GGWCC 1 cut(s) 68
AxyI CCTNAGG 1 cut(s) 719
BaeGI GKGCMC 1 cut(s) 249
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 639
BccI CCATC 2 cut(s) 202, 842
BceAI ACGGC 1 cut(s) 80
BciT130I CCWGG 2 cut(s) 347, 699
BciVI GTATCC 1 cut(s) 110
BcnI CCSGG 1 cut(s) 897
BfaI CTAG 2 cut(s) 540, 747
BfmI CTRYAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 624
BfuI GTATCC 1 cut(s) 110
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 351, 736
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 2 cut(s) 352, 737
Bme1390I CCNGG 3 cut(s) 347, 699, 897
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 68, 352
BmiI GGNNCC 3 cut(s) 354, 711, 828
BmrFI CCNGG 3 cut(s) 347, 699, 897
BmsI GCATC 2 cut(s) 473, 805
BmtI GCTAGC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 548
Bpu10I CCTNAGC 1 cut(s) 639
Bpu1102I GCTNAGC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 897
BsaJI CCNNGG 2 cut(s) 572, 896
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse1I ACTGG 4 cut(s) 70, 144, 531, 764
Bse21I CCTNAGG 1 cut(s) 719
BseBI CCWGG 2 cut(s) 347, 699
BseDI CCNNGG 2 cut(s) 572, 896
BseGI GGATG 2 cut(s) 194, 220
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMII CTCAG 6 cut(s) 15, 92, 147, 630, 710, 858
BseNI ACTGG 4 cut(s) 70, 144, 531, 764
BseSI GKGCMC 1 cut(s) 249
BshFI GGCC 2 cut(s) 95, 353
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 896
BslI CCNNNNNNNGG 1 cut(s) 578
BsmI GAATGC 3 cut(s) 404, 640, 790
BsnI GGCC 2 cut(s) 95, 353
Bsp1286I GDGCHC 1 cut(s) 249
Bsp1720I GCTNAGC 1 cut(s) 78
BspACI CCGC 3 cut(s) 290, 350, 736
BspANI GGCC 2 cut(s) 95, 353
BspCNI CTCAG 6 cut(s) 16, 91, 148, 631, 711, 859
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 354, 711, 828
BspMI ACCTGC 1 cut(s) 624
BspOI GCTAGC 1 cut(s) 750
BsrI ACTGG 4 cut(s) 70, 144, 531, 764
BssECI CCNNGG 2 cut(s) 572, 896
BssNAI GTATAC 1 cut(s) 88
Bst1107I GTATAC 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 347, 699
Bst4CI ACNGT 1 cut(s) 611
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 32, 361, 748
BstDEI CTNAG 6 cut(s) 24, 78, 156, 639, 719, 867
BstDSI CCRYGG 1 cut(s) 572
BstF5I GGATG 2 cut(s) 194, 220
BstMWI GCNNNNNNNGC 3 cut(s) 140, 266, 356
BstNI CCWGG 2 cut(s) 347, 699
BstSCI CCNGG 3 cut(s) 345, 697, 895
BstSFI CTRYAG 1 cut(s) 335
BstSLI GKGCMC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 65
BstZ17I GTATAC 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 719
BsuI GTATCC 1 cut(s) 110
BsuRI GGCC 2 cut(s) 95, 353
BtgI CCRYGG 1 cut(s) 572
BtsCI GGATG 2 cut(s) 194, 220
BtsIMutI CAGTG 1 cut(s) 137
BveI ACCTGC 1 cut(s) 624
Cac8I GCNNGC 3 cut(s) 32, 361, 748
CciI TCATGA 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 68, 352
CseI GACGC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 4, 314
CviAII CATG 3 cut(s) 47, 692, 820
CviQI GTAC 2 cut(s) 4, 314
DdeI CTNAG 6 cut(s) 24, 78, 156, 639, 719, 867
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 876
Eco81I CCTNAGG 1 cut(s) 719
EcoRII CCWGG 2 cut(s) 345, 697
FaeI CATG 3 cut(s) 50, 695, 823
FatI CATG 3 cut(s) 46, 691, 819
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 351, 736
FokI GGATG 2 cut(s) 181, 207
Fsp4HI GCNGC 2 cut(s) 351, 736
FspBI CTAG 2 cut(s) 540, 747
GluI GCNGC 2 cut(s) 351, 736
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 2 cut(s) 95, 353
HapII CCGG 1 cut(s) 896
HgaI GACGC 1 cut(s) 8
Hin1II CATG 3 cut(s) 50, 695, 823
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 5 cut(s) 386, 426, 584, 730, 887
HpaII CCGG 1 cut(s) 896
HphI GGTGA 2 cut(s) 560, 605
Hpy166II GTNNAC 4 cut(s) 88, 247, 551, 607
Hpy188I TCNGA 3 cut(s) 729, 856, 868
Hpy188III TCNNGA 7 cut(s) 23, 47, 58, 623, 718, 812, 884
Hpy8I GTNNAC 4 cut(s) 88, 247, 551, 607
HpyAV CCTTC 2 cut(s) 817, 873
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 5 cut(s) 247, 260, 451, 464, 805
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 266, 356
HpyF3I CTNAG 6 cut(s) 24, 78, 156, 639, 719, 867
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 3 cut(s) 50, 695, 823
LmnI GCTCC 2 cut(s) 715, 826
LweI GCATC 2 cut(s) 473, 805
MaeI CTAG 2 cut(s) 540, 747
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 611
MboII GAAGA 5 cut(s) 73, 197, 320, 560, 883
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 6 cut(s) 50, 179, 232, 672, 753, 769
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 5 cut(s) 107, 145, 634, 662, 714
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 2 cut(s) 381, 630
MslI CAYNNNNRTG 1 cut(s) 368
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 3 cut(s) 347, 699, 897
Mva1269I GAATGC 3 cut(s) 404, 640, 790
MvaI CCWGG 2 cut(s) 347, 699
MwoI GCNNNNNNNGC 3 cut(s) 140, 266, 356
NciI CCSGG 1 cut(s) 897
NheI GCTAGC 1 cut(s) 746
NlaIII CATG 3 cut(s) 50, 695, 823
NlaIV GGNNCC 3 cut(s) 354, 711, 828
NmuCI GTSAC 1 cut(s) 611
PagI TCATGA 1 cut(s) 46
PaqCI CACCTGC 1 cut(s) 624
PctI GAATGC 3 cut(s) 404, 640, 790
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 5 cut(s) 386, 426, 584, 730, 887
PkrI GCNGC 2 cut(s) 352, 737
Psp6I CCWGG 2 cut(s) 345, 697
PspGI CCWGG 2 cut(s) 345, 697
PspN4I GGNNCC 3 cut(s) 354, 711, 828
PspPI GGNCC 2 cut(s) 68, 352
RsaI GTAC 2 cut(s) 5, 315
RsaNI GTAC 2 cut(s) 4, 314
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 2 cut(s) 381, 630
SatI GCNGC 2 cut(s) 351, 736
Sau96I GGNCC 2 cut(s) 68, 352
ScrFI CCNGG 3 cut(s) 347, 699, 897
SduI GDGCHC 1 cut(s) 249
SetI ASST 9 cut(s) 32, 271, 311, 368, 483, 618, 645, 837, 865
SfaNI GCATC 2 cut(s) 473, 805
SfcI CTRYAG 1 cut(s) 335
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 368
Sse9I AATT 6 cut(s) 50, 179, 232, 672, 753, 769
SsiI CCGC 3 cut(s) 290, 350, 736
SspMI CTAG 2 cut(s) 540, 747
StyD4I CCNGG 3 cut(s) 345, 697, 895
TaaI ACNGT 1 cut(s) 611
TaiI ACGT 1 cut(s) 311
TasI AATT 6 cut(s) 50, 179, 232, 672, 753, 769
TauI GCSGC 2 cut(s) 353, 738
TfiI GAWTC 5 cut(s) 386, 426, 584, 730, 887
Tru1I TTAA 2 cut(s) 381, 630
Tru9I TTAA 2 cut(s) 381, 630
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 611
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 63, 209, 836
TspRI CASTG 1 cut(s) 144
VneI GTGCAC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 2 cut(s) 50, 232
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 2 cut(s) 540, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.