Rh1DG380400

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012420
Physical Location & Seq
Forward (+)
60196710 .. 60245183
48474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG380400.1

Sequence Viewer

Length: 2940 bp
ATGCCTGAGTGGGTTTTCAGTTTCAGAAATCTGGTGTCTCTTTCTCTTAGAGATTGTGGTTTCCAAGGTCCAATTCCTAGCAGTAGCAGCCCACACAATATCACATCTCTCAGGGAGATTGATCTCTCAATTAATCACCTTAGGGATCCGATACCGAAATGGCTGCTCAACAACAAAGACCTTACTGTACTTGATCTAGGATACAATTCTTATACAGGACCAATTCCAGGTGGCATTGTGAATATGACTAGTCTAAAGGATCTTCATCTCGAGTCTAATTCTTTCATCTCTACCATACCTGAATGGTTGTCTAGTTTTAATAATCTTGAATCCTTGGATATTTCTAGCAATCACTTGCATGGTGAAATTTTGAGTTCCATTGGAAACTTGACATCCATTGTACATCTTGGTTTAGGTTTTAATCAGTTTGAAGGGACAATCCCAAACTCCTTGGGCAATCTGTCTTCCTTAGAGTCATTACAGATTTCATACAATTCGTTTCATGGAACTCTTCCAGAGACCATTGGCCAACTCAAAAGGCTAACAGCTTTGGATATATCTTTTAATTCATTTGAAGGTGTAGTGTCTGAAGTTCATTTTACACATCTTACAAGTTTAAAATCCTTCAGAGCACATGGAAACTCATTGACTCTAAAAACTAGTCGAGACTGGCTTCCTCCTTTTCAACTTCGAAGCTTGCATTTAGATTCTTGGCATTTGGGGCTTGAACTGCCTTCATGGCTTCGAACACAAACACAATTGCTGGAGCTAAGCCTATCTGGTACAGGAATTTCAGGTACCATTCCCACCTGGTGTTGGAACTTTACTTCCGAACTAGTTTACCTGGATCTGTCCAACAATCAATTACATGGGGAGATTCAACATATAAATGTTGGTGCCCCATTCTCAATAATTGACCTGAGTTCAAACCAGTTCAACGATCATATTGCAGCAGCATCAGACCAAACCCGGATCGGCCAGACCCAACCGCAACAGGTGATCGGTGTAGAGAGATCCCAGCCACCACCGGCCTCCAATCATCTTGAAGGACCACTTCCAGGAGGATTCTCAAATATGACTAGTCTTAAGGTTCTTAATGTCGACTTTAACTTTTTCAATTCTACCATACCTGAGTCATTGTATAGTTTGAACTATCTTGAATCTCTAGTTCTTTCCACAAATGATTTGGATGGGGAAATTTCGAGTTCCATTGGAAACTTGACGTCCATTGTTGAACTTCAGTTACAAATGAATCGGTTGGGAGGGAAAGTCCCAGAATCATTGGGAAATCTGTGTAACTTGATGGATCTCGATCTATCAGTTAACAATTTCAACGGAAATGGATCAGAATTCCTTGAAAGTTTGTCTGCATGTAGTTCACATCATATCGAATCATTGCGGTTAAATAGTAACAATTTTTCTGGTCATTTAACAGCCCAAATAGGAAATTTAACAAGTTTAAGCTTGCTTGATCTTGGTTACAATCAGTTTGATGGAGCTCTGCCAGAAAGCATTGGCCAACTCAAAAGGCTAAACCAATTGGTTATACATCATAATTCATTTCAAGGTATTGGGCTATCGGCAGTGCAGAAGAAAATAGCAGGACCTCCATCGTTTGCCAAAGGCTTAGCTGCTCAAACGTTAGGTTGGCTCTGCAGAACAAAAAAGGAGGAAGATCCTCCAAACTTGCAGCAACTTGTCTCGGAAAAAGATTTGATTGTGAGAGAAGAAGCATGCCGAATTGGCTTATTTTTTGGTGGATTTAGTGGCTCTTACCATGCTATTAGATGCTTGTTGAGAAAGTACAGAAAGAAAGAGACACCATTGAATGCAATCTTAGCAGGTTCAATTGCGGGGTTGTCTATTTTAGCATTAGATGACTCAAACCGAAGGCGGACACTATCTCTGTACTTGTTGGCAAGGGTAGCACAGTCTGCTTATAATTCTGCAAAGTCTAAGAACAAGTTTCACTTTTGGGGAAGCCACTGGAGGCATGGGGATTCTCTGCTCTTTTCTATTGCATGTGCACAGGTTATGTACGCCTTTGTAATGCGTCCTGAGAGCTTGCCTAAATCTTATCATGAATTTATCCAGAAGACTGGTCCTGTTGCTCAGCCTGTATACAAGGCCGTAAGGGAGTGTTGTAGAGGATACCGAGTGGATATTGCCTCACTGGCTGATTACTTGGCTGAGAAAGGGAGAAGCGATATAAAATTGGAAGAGTTTCCATCCATTTTGCCTTGTTCTGTTATTCATCCCCACACAAATTCTTGTTTAGTGCACAACGCAACTGCAACATCAGCTACATTTAGGAAAACATTTCCGCTTTACTTCTCTTTGACGTTTGTACCATTTGTCGTTCTTCGCCTACAGAAGTTCCTGGCGGCCCCTGCTCGCACCTTTATGTTTGCTGTTAAAGATTCTGTTCGCTCAACAGCATTCTTGTCTGCTTTTGTTGGAATCTTTCAGGGTGTGATATGTTTGCACAGAAAAGTTGCATCAAAAGACAACAAGCTTGTATATTGGATTGCTGGTGGTCTATCTGCTCTTTCAGTTCTACTGGAGAAAAAGGCTAGGCGTGGTGAACTTGCTCTATATGTTCTTCCACGGGCAGGAGATTCTTTGTGGTATATTTTAGTAAACCGTCACCTGCTTCCAGATATTAAGAATGCTGAGGTGTTCTTGTTCTGTTTGTGTATGGGAGGAATTATGTATTACTTGGAACATGAGCCAGGCACTATGGCTCCATTCCTGAGGGGTCTGATTCGCCGCTTTCTTGCTAGCCGAATTAGTAATCCAGTCAATTCGTCTAATAGGAATGCTTCCTACACATATTTGCAAACTCTTGATGCCATGAAGGAGCCAAAGCTATTAGAGAATGATGGTTCTGAAGCACCTTCTGAGAAGAAATACAATCTTGAATCAATACCGGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

979

Amino Acids

108.5

Weight (kDa)

7.4

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 36 - 94 5.8e-08 Leucine rich repeat
LRR_14 PF23598 59 - 258 9e-18 Leucine-rich repeat region
LRR_8 PF13855 134 - 190 7.3e-06 Leucine rich repeat
LRR_8 PF13855 156 - 216 2.9e-06 Leucine rich repeat
LRR_14 PF23598 352 - 472 3e-06 Leucine-rich repeat region
Tim17 PF02466 574 - 621 5.4e-08 Tim17/Tim22/Tim23/Pmp24 family
TMEM135_C_rich PF15982 750 - 878 4e-08 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1941
AarI CACCTGC 1 cut(s) 2658
AatII GACGTC 1 cut(s) 1226
Acc36I ACCTGC 2 cut(s) 1832, 2658
Acc65I GGTACC 1 cut(s) 797
AccB1I GGYRCC 2 cut(s) 797, 896
AccI GTMKAC 2 cut(s) 1101, 2121
AciI CCGC 7 cut(s) 989, 1399, 1853, 1894, 2324, 2384, 2770
AclI AACGTT 1 cut(s) 1640
AclWI GGATC 9 cut(s) 140, 153, 267, 855, 980, 1008, 1314, 1351, 1670
AcoI YGGCCR 3 cut(s) 526, 976, 1516
AcsI RAATTY 7 cut(s) 366, 789, 1197, 1349, 1447, 2084, 2266
AcuI CTGAAG 4 cut(s) 609, 610, 1223, 2910
AcyI GRCGYC 1 cut(s) 1223
AdeI CACNNNGTG 1 cut(s) 813
AfaI GTAC 8 cut(s) 189, 402, 784, 799, 1805, 1910, 2039, 2349
AfiI CCNNNNNNNGG 4 cut(s) 227, 816, 1058, 2612
AflII CTTAAG 1 cut(s) 1085
AhlI ACTAGT 4 cut(s) 248, 659, 835, 1079
AjnI CCWGG 6 cut(s) 226, 809, 843, 1057, 2379, 2731
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AloI GAACNNNNNNTCC 2 cut(s) 812, 844
Alw21I GWGCWC 4 cut(s) 634, 1501, 2029, 2283
Alw26I GTCTC 5 cut(s) 42, 512, 660, 1705, 1811
Alw44I GTGCAC 2 cut(s) 2025, 2279
AlwI GGATC 9 cut(s) 140, 153, 267, 855, 980, 1008, 1314, 1351, 1670
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 6 cut(s) 526, 976, 1029, 1516, 2127, 2385
ApaLI GTGCAC 2 cut(s) 2025, 2279
ApeKI GCWGC 6 cut(s) 87, 163, 950, 953, 1631, 1690
ApoI RAATTY 7 cut(s) 366, 789, 1197, 1349, 1447, 2084, 2266
AseI ATTAAT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 2223
Asp718I GGTACC 1 cut(s) 797
AspS9I GGNCC 6 cut(s) 68, 218, 1049, 1604, 2102, 2386
AsuC2I CCSGG 2 cut(s) 970, 2931
AsuHPI GGTGA 5 cut(s) 128, 374, 1009, 2594, 2639
AsuII TTCGAA 2 cut(s) 691, 745
AsuNHI GCTAGC 1 cut(s) 2780
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 5 cut(s) 68, 218, 1049, 1604, 2102
AxyI CCTNAGG 2 cut(s) 140, 2753
BaeGI GKGCMC 3 cut(s) 901, 2029, 2283
BaeI ACNNNNGTAYC 2 cut(s) 2331, 2364
BalI TGGCCA 2 cut(s) 528, 1518
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 2 cut(s) 797, 896
BanII GRGCYC 1 cut(s) 1501
BbsI GAAGAC 2 cut(s) 456, 2102
Bbv12I GWGCWC 4 cut(s) 634, 1501, 2029, 2283
BbvCI CCTCAGC 1 cut(s) 2673
BbvI GCAGC 6 cut(s) 99, 150, 962, 965, 1618, 1702
BccI CCATC 6 cut(s) 1184, 1297, 1487, 1618, 2236, 2876
BceAI ACGGC 1 cut(s) 2114
BcgI CGANNNNNNTGC 4 cut(s) 145, 179, 929, 963
BciT130I CCWGG 6 cut(s) 228, 811, 845, 1059, 2381, 2733
BciVI GTATCC 2 cut(s) 194, 2144
BcnI CCSGG 2 cut(s) 970, 2931
BcoDI GTCTC 5 cut(s) 42, 512, 660, 1705, 1811
BcuI ACTAGT 4 cut(s) 248, 659, 835, 1079
BfmI CTRYAG 2 cut(s) 1654, 2369
BfrI CTTAAG 1 cut(s) 1085
BfuAI ACCTGC 2 cut(s) 1832, 2658
BfuI GTATCC 2 cut(s) 194, 2144
BglI GCCNNNNNGGC 3 cut(s) 739, 1743, 2174
BisI GCNGC 8 cut(s) 88, 164, 951, 954, 1632, 1691, 2385, 2770
BlpI GCTNAGC 3 cut(s) 770, 1627, 2112
BlsI GCNGC 8 cut(s) 89, 165, 952, 955, 1633, 1692, 2386, 2771
Bme1390I CCNGG 8 cut(s) 228, 811, 845, 970, 1059, 2381, 2733, 2931
Bme18I GGWCC 5 cut(s) 68, 218, 1049, 1604, 2102
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 68, 218, 1049, 1604, 2102, 2386
BmiI GGNNCC 6 cut(s) 147, 799, 898, 2388, 2745, 2862
BmrFI CCNGG 8 cut(s) 228, 811, 845, 970, 1059, 2381, 2733, 2931
BmsI GCATC 4 cut(s) 965, 1778, 2507, 2839
BmtI GCTAGC 1 cut(s) 2784
BpiI GAAGAC 2 cut(s) 456, 2102
BpmI CTGGAG 3 cut(s) 785, 2008, 2582
Bpu10I CCTNAGC 1 cut(s) 2673
Bpu1102I GCTNAGC 3 cut(s) 770, 1627, 2112
Bpu14I TTCGAA 2 cut(s) 691, 745
BpuMI CCSGG 2 cut(s) 970, 2931
BsaBI GATNNNNATC 2 cut(s) 264, 1311
BsaHI GRCGYC 1 cut(s) 1223
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 5 cut(s) 64, 333, 450, 2606, 2930
BsaXI ACNNNNNCTCC 4 cut(s) 1254, 1284, 2728, 2758
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 816, 1058, 2612
Bse118I RCCGGY 1 cut(s) 1027
Bse1I ACTGG 7 cut(s) 674, 931, 1991, 2104, 2178, 2565, 2798
Bse21I CCTNAGG 2 cut(s) 140, 2753
Bse3DI GCAATG 1 cut(s) 1394
Bse8I GATNNNNATC 2 cut(s) 264, 1311
BseBI CCWGG 6 cut(s) 228, 811, 845, 1059, 2381, 2733
BseDI CCNNGG 5 cut(s) 64, 333, 450, 2606, 2930
BseGI GGATG 4 cut(s) 392, 1195, 2228, 2254
BseJI GATNNNNATC 2 cut(s) 264, 1311
BseLI CCNNNNNNNGG 4 cut(s) 227, 816, 1058, 2612
BseMI GCAATG 1 cut(s) 1394
BseMII CTCAG 9 cut(s) 124, 911, 1122, 2049, 2126, 2181, 2664, 2744, 2892
BseNI ACTGG 7 cut(s) 674, 931, 1991, 2104, 2178, 2565, 2798
BseSI GKGCMC 3 cut(s) 901, 2029, 2283
BseXI GCAGC 6 cut(s) 99, 150, 962, 965, 1618, 1702
BseYI CCCAGC 1 cut(s) 1017
BsgI GTGCAG 1 cut(s) 1607
BshFI GGCC 6 cut(s) 528, 978, 1031, 1518, 2129, 2387
BshNI GGYRCC 2 cut(s) 797, 896
BsiHKAI GWGCWC 4 cut(s) 634, 1501, 2029, 2283
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 3 cut(s) 970, 1028, 2930
BslFI GGGAC 2 cut(s) 448, 1256
BslI CCNNNNNNNGG 4 cut(s) 227, 816, 1058, 2612
BsmAI GTCTC 5 cut(s) 42, 512, 660, 1705, 1811
BsmFI GGGAC 2 cut(s) 448, 1256
BsmI GAATGC 4 cut(s) 1834, 2438, 2674, 2824
BsnI GGCC 6 cut(s) 528, 978, 1031, 1518, 2129, 2387
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 2 cut(s) 691, 745
Bsp1286I GDGCHC 5 cut(s) 634, 901, 1501, 2029, 2283
Bsp1407I TGTACA 1 cut(s) 400
Bsp1720I GCTNAGC 3 cut(s) 770, 1627, 2112
BspACI CCGC 7 cut(s) 989, 1399, 1853, 1894, 2324, 2384, 2770
BspANI GGCC 6 cut(s) 528, 978, 1031, 1518, 2129, 2387
BspCNI CTCAG 9 cut(s) 123, 912, 1123, 2050, 2125, 2182, 2665, 2745, 2893
BspHI TCATGA 1 cut(s) 2080
BspLI GGNNCC 6 cut(s) 147, 799, 898, 2388, 2745, 2862
BspMAI CTGCAG 1 cut(s) 1658
BspMI ACCTGC 2 cut(s) 1832, 2658
BspOI GCTAGC 1 cut(s) 2784
BspPI GGATC 9 cut(s) 140, 153, 267, 855, 980, 1008, 1314, 1351, 1670
BspT104I TTCGAA 2 cut(s) 691, 745
BspT107I GGYRCC 2 cut(s) 797, 896
BspTI CTTAAG 1 cut(s) 1085
BspTNI GGTCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 1394
BsrFI RCCGGY 1 cut(s) 1027
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 7 cut(s) 674, 931, 1991, 2104, 2178, 2565, 2798
BssAI RCCGGY 1 cut(s) 1027
BssECI CCNNGG 5 cut(s) 64, 333, 450, 2606, 2930
BssNAI GTATAC 1 cut(s) 2122
BssNI GRCGYC 1 cut(s) 1223
BssT1I CCWWGG 3 cut(s) 64, 333, 450
Bst1107I GTATAC 1 cut(s) 2122
Bst2UI CCWGG 6 cut(s) 228, 811, 845, 1059, 2381, 2733
Bst4CI ACNGT 3 cut(s) 187, 1932, 2645
Bst6I CTCTTC 2 cut(s) 516, 2214
BstACI GRCGYC 1 cut(s) 1223
BstAFI CTTAAG 1 cut(s) 1085
BstAPI GCANNNNNTGC 1 cut(s) 1934
BstAUI TGTACA 1 cut(s) 400
BstBI TTCGAA 2 cut(s) 691, 745
BstC8I GCNNGC 6 cut(s) 698, 1466, 1735, 2066, 2395, 2782
BstDSI CCRYGG 1 cut(s) 2606
BstF5I GGATG 4 cut(s) 392, 1195, 2228, 2254
BstMAI GTCTC 5 cut(s) 42, 512, 660, 1705, 1811
BstNI CCWGG 6 cut(s) 228, 811, 845, 1059, 2381, 2733
BstNSI RCATGY 3 cut(s) 1374, 1737, 2025
BstSCI CCNGG 8 cut(s) 226, 809, 843, 968, 1057, 2379, 2731, 2929
BstSFI CTRYAG 2 cut(s) 1654, 2369
BstSLI GKGCMC 3 cut(s) 901, 2029, 2283
BstV1I GCAGC 6 cut(s) 99, 150, 962, 965, 1618, 1702
BstV2I GAAGAC 2 cut(s) 456, 2102
BstX2I RGATCY 6 cut(s) 145, 259, 847, 1013, 1306, 1675
BstXI CCANNNNNNTGG 1 cut(s) 2099
BstYI RGATCY 6 cut(s) 145, 259, 847, 1013, 1306, 1675
BstZ17I GTATAC 1 cut(s) 2122
Bsu36I CCTNAGG 2 cut(s) 140, 2753
BsuI GTATCC 2 cut(s) 194, 2144
BsuRI GGCC 6 cut(s) 528, 978, 1031, 1518, 2129, 2387
BtgI CCRYGG 1 cut(s) 2606
BtsCI GGATG 4 cut(s) 392, 1195, 2228, 2254
BtsI GCAGTG 1 cut(s) 1590
BtsIMutI CAGTG 3 cut(s) 1590, 1984, 2171
BveI ACCTGC 2 cut(s) 1832, 2658
Cac8I GCNNGC 6 cut(s) 698, 1466, 1735, 2066, 2395, 2782
CciI TCATGA 1 cut(s) 2080
Cfr10I RCCGGY 1 cut(s) 1027
Cfr13I GGNCC 6 cut(s) 68, 218, 1049, 1604, 2102, 2386
CseI GACGC 1 cut(s) 2042
CsiI ACCWGGT 1 cut(s) 809
Csp6I GTAC 8 cut(s) 188, 401, 783, 798, 1804, 1909, 2038, 2348
CviQI GTAC 8 cut(s) 188, 401, 783, 798, 1804, 1909, 2038, 2348
DraI TTTAAA 1 cut(s) 618
DraIII CACNNNGTG 1 cut(s) 813
EaeI YGGCCR 3 cut(s) 526, 976, 1516
Eam1104I CTCTTC 2 cut(s) 516, 2214
EarI CTCTTC 2 cut(s) 516, 2214
EciI GGCGGA 1 cut(s) 1909
Ecl136II GAGCTC 1 cut(s) 1499
Eco130I CCWWGG 3 cut(s) 64, 333, 450
Eco24I GRGCYC 1 cut(s) 1501
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 5 cut(s) 68, 218, 1049, 1604, 2102
Eco53kI GAGCTC 1 cut(s) 1499
Eco57I CTGAAG 4 cut(s) 609, 610, 1223, 2910
Eco81I CCTNAGG 2 cut(s) 140, 2753
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 1499
EcoO109I RGGNCCY 1 cut(s) 1604
EcoRI GAATTC 1 cut(s) 1349
EcoRII CCWGG 6 cut(s) 226, 809, 843, 1057, 2379, 2731
EcoT14I CCWWGG 3 cut(s) 64, 333, 450
EcoT38I GRGCYC 1 cut(s) 1501
ErhI CCWWGG 3 cut(s) 64, 333, 450
FalI AAGNNNNNCTT 4 cut(s) 1038, 1070, 1955, 1987
FaqI GGGAC 2 cut(s) 448, 1256
FauI CCCGC 1 cut(s) 1846
FblI GTMKAC 2 cut(s) 1101, 2121
Fnu4HI GCNGC 8 cut(s) 88, 164, 951, 954, 1632, 1691, 2385, 2770
FokI GGATG 4 cut(s) 379, 1202, 2215, 2241
FriOI GRGCYC 1 cut(s) 1501
Fsp4HI GCNGC 8 cut(s) 88, 164, 951, 954, 1632, 1691, 2385, 2770
GluI GCNGC 8 cut(s) 88, 164, 951, 954, 1632, 1691, 2385, 2770
GsaI CCCAGC 1 cut(s) 1021
GsuI CTGGAG 3 cut(s) 785, 2008, 2582
HaeIII GGCC 6 cut(s) 528, 978, 1031, 1518, 2129, 2387
HapII CCGG 3 cut(s) 970, 1028, 2930
HgaI GACGC 1 cut(s) 2042
Hin1I GRCGYC 1 cut(s) 1223
HincII GTYRAC 2 cut(s) 1102, 1324
HindII GTYRAC 2 cut(s) 1102, 1324
HindIII AAGCTT 3 cut(s) 694, 1462, 2513
HpaI GTTAAC 1 cut(s) 1324
HpaII CCGG 3 cut(s) 970, 1028, 2930
HphI GGTGA 5 cut(s) 128, 374, 1009, 2594, 2639
Hpy166II GTNNAC 9 cut(s) 841, 1102, 1324, 1379, 2027, 2122, 2281, 2585, 2641
Hpy8I GTNNAC 9 cut(s) 841, 1102, 1324, 1379, 2027, 2122, 2281, 2585, 2641
HpyAV CCTTC 8 cut(s) 425, 569, 634, 744, 1040, 1884, 2851, 2907
HpyCH4III ACNGT 3 cut(s) 187, 1932, 2645
HpyCH4IV ACGT 3 cut(s) 1223, 1640, 2342
HpySE526I ACGT 3 cut(s) 1223, 1640, 2342
Hsp92I GRCGYC 1 cut(s) 1223
KpnI GGTACC 1 cut(s) 801
KspAI GTTAAC 1 cut(s) 1324
LmnI GCTCC 4 cut(s) 766, 1496, 2749, 2860
Lsp1109I GCAGC 6 cut(s) 99, 150, 962, 965, 1618, 1702
LweI GCATC 4 cut(s) 965, 1778, 2507, 2839
MabI ACCWGGT 1 cut(s) 809
MaeII ACGT 3 cut(s) 1223, 1640, 2342
MaeIII GTNAC 5 cut(s) 1242, 1295, 1409, 1478, 2645
MfeI CAATTG 3 cut(s) 758, 1538, 1848
MflI RGATCY 6 cut(s) 145, 259, 847, 1013, 1306, 1675
MhlI GDGCHC 5 cut(s) 634, 901, 1501, 2029, 2283
MlsI TGGCCA 2 cut(s) 528, 1518
MluNI TGGCCA 2 cut(s) 528, 1518
MlyI GAGTC 5 cut(s) 281, 482, 643, 1142, 1874
MmeI TCCRAC 3 cut(s) 797, 879, 2437
Mox20I TGGCCA 2 cut(s) 528, 1518
MroXI GAANNNNTTC 1 cut(s) 2223
MscI TGGCCA 2 cut(s) 528, 1518
MslI CAYNNNNRTG 3 cut(s) 357, 888, 2402
Msp20I TGGCCA 2 cut(s) 528, 1518
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 3 cut(s) 970, 1028, 2930
MspR9I CCNGG 8 cut(s) 228, 811, 845, 970, 1059, 2381, 2733, 2931
MunI CAATTG 3 cut(s) 758, 1538, 1848
Mva1269I GAATGC 4 cut(s) 1834, 2438, 2674, 2824
MvaI CCWGG 6 cut(s) 228, 811, 845, 1059, 2381, 2733
NciI CCSGG 2 cut(s) 970, 2931
NheI GCTAGC 1 cut(s) 2780
NlaIV GGNNCC 6 cut(s) 147, 799, 898, 2388, 2745, 2862
NmuCI GTSAC 1 cut(s) 2645
NspI RCATGY 3 cut(s) 1374, 1737, 2025
NspV TTCGAA 2 cut(s) 691, 745
PaeI GCATGC 1 cut(s) 1737
PaeR7I CTCGAG 1 cut(s) 269
PagI TCATGA 1 cut(s) 2080
PaqCI CACCTGC 1 cut(s) 2658
PctI GAATGC 4 cut(s) 1834, 2438, 2674, 2824
PdmI GAANNNNTTC 1 cut(s) 2223
PfoI TCCNGGA 1 cut(s) 1057
PkrI GCNGC 8 cut(s) 89, 165, 952, 955, 1633, 1692, 2386, 2771
PleI GAGTC 5 cut(s) 280, 481, 643, 1141, 1874
PpsI GAGTC 5 cut(s) 280, 481, 643, 1141, 1874
PpuMI RGGWCCY 1 cut(s) 1604
PshBI ATTAAT 1 cut(s) 132
PsiI TTATAA 1 cut(s) 1941
Psp124BI GAGCTC 1 cut(s) 1501
Psp1406I AACGTT 1 cut(s) 1640
Psp5II RGGWCCY 1 cut(s) 1604
Psp6I CCWGG 6 cut(s) 226, 809, 843, 1057, 2379, 2731
PspFI CCCAGC 1 cut(s) 1017
PspGI CCWGG 6 cut(s) 226, 809, 843, 1057, 2379, 2731
PspN4I GGNNCC 6 cut(s) 147, 799, 898, 2388, 2745, 2862
PspPI GGNCC 6 cut(s) 68, 218, 1049, 1604, 2102, 2386
PspPPI RGGWCCY 1 cut(s) 1604
PsrI GAACNNNNNNTAC 2 cut(s) 1227, 1259
PstI CTGCAG 1 cut(s) 1658
PsuI RGATCY 6 cut(s) 145, 259, 847, 1013, 1306, 1675
RsaI GTAC 8 cut(s) 189, 402, 784, 799, 1805, 1910, 2039, 2349
RsaNI GTAC 8 cut(s) 188, 401, 783, 798, 1804, 1909, 2038, 2348
RseI CAYNNNNRTG 3 cut(s) 357, 888, 2402
SacI GAGCTC 1 cut(s) 1501
SalI GTCGAC 1 cut(s) 1100
SatI GCNGC 8 cut(s) 88, 164, 951, 954, 1632, 1691, 2385, 2770
Sau96I GGNCC 6 cut(s) 68, 218, 1049, 1604, 2102, 2386
SchI GAGTC 5 cut(s) 281, 482, 643, 1142, 1874
ScrFI CCNGG 8 cut(s) 228, 811, 845, 970, 1059, 2381, 2733, 2931
SduI GDGCHC 5 cut(s) 634, 901, 1501, 2029, 2283
SexAI ACCWGGT 1 cut(s) 809
SfaNI GCATC 4 cut(s) 965, 1778, 2507, 2839
SfcI CTRYAG 2 cut(s) 1654, 2369
Sfr274I CTCGAG 1 cut(s) 269
SfuI TTCGAA 2 cut(s) 691, 745
SinI GGWCC 5 cut(s) 68, 218, 1049, 1604, 2102
SlaI CTCGAG 1 cut(s) 269
SmiMI CAYNNNNRTG 3 cut(s) 357, 888, 2402
SmlI CTYRAG 2 cut(s) 269, 1085
SmoI CTYRAG 2 cut(s) 269, 1085
SpeI ACTAGT 4 cut(s) 248, 659, 835, 1079
SphI GCATGC 1 cut(s) 1737
SsiI CCGC 7 cut(s) 989, 1399, 1853, 1894, 2324, 2384, 2770
SstI GAGCTC 1 cut(s) 1501
StyD4I CCNGG 8 cut(s) 226, 809, 843, 968, 1057, 2379, 2731, 2929
StyI CCWWGG 3 cut(s) 64, 333, 450
TaaI ACNGT 3 cut(s) 187, 1932, 2645
TaiI ACGT 3 cut(s) 1226, 1643, 2345
TaqI TCGA 8 cut(s) 270, 664, 691, 745, 1101, 1202, 1311, 1389
TatI WGTACW 4 cut(s) 187, 400, 1803, 1908
TauI GCSGC 2 cut(s) 2387, 2772
TscAI CASTG 3 cut(s) 1590, 1991, 2178
TseFI GTSAC 1 cut(s) 2645
TseI GCWGC 6 cut(s) 87, 163, 950, 953, 1631, 1690
Tsp45I GTSAC 1 cut(s) 2645
TspGWI ACGGA 1 cut(s) 1350
TspRI CASTG 3 cut(s) 1590, 1991, 2178
Vha464I CTTAAG 1 cut(s) 1085
VneI GTGCAC 2 cut(s) 2025, 2279
VpaK11BI GGWCC 5 cut(s) 68, 218, 1049, 1604, 2102
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 7 cut(s) 366, 789, 1197, 1349, 1447, 2084, 2266
XceI RCATGY 3 cut(s) 1374, 1737, 2025
XcmI CCANNNNNNNNNTGG 2 cut(s) 1183, 1991
XhoI CTCGAG 1 cut(s) 269
XmiI GTMKAC 2 cut(s) 1101, 2121
XmnI GAANNNNTTC 1 cut(s) 2223
ZraI GACGTC 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.