Rmu_sc0031315.1_g000001

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0031315.1
OG0012420
Physical Location & Seq
Forward (+)
4632 .. 6325
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0031315.1_g000001.1.cds

Sequence Viewer

Length: 636 bp
atgaccaaaatgacatttctgagtcgcttgaacttgtcatacaacaatttgacgggacggattccagaaagcactcaacttcagagccttgatcaatccagctttgttggcaatgaactttgtggtcctccactcatcaagaattgcagtgcaagcaaggtgacaccaccaacagttgagcaacagagaggatatgatttacttgacgacaagtggttctacctgagcttgggattgggattcgcggttgatgccaagcccaaaccccaaggcccaagctttctcatcctcctctcccccgcaatgccgtcgccgtcgtctctccccaaacccccgacctccccgccgccgcccctagacgccgccgcctccgaccccctccgcgaggccgaggaccgcctccgcgacgccattgaggagctccagcgccgccagcgcaccgccgcagcccgtggctcccagcaccaccccccctgcgaccacgccaacgacgagtcgtgcgtcgcccacgccattgggaacctctgccagagctttctcctctcttatggcgtccgagttgggattgggattcttcttcgcgcgtttaagctcgctcgcagacaatcctactcttccttgctcgatcttaaggta
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

212

Amino Acids

23.19

Weight (kDa)

8.62

Isoelectric Point (pI)

61.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 245, 384, 405, 582, 584
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 3 cut(s) 360, 408, 552
AfiI CCNNNNNNNGG 2 cut(s) 229, 385
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 1 cut(s) 31
AluBI AGCT 6 cut(s) 102, 228, 279, 421, 534, 592
AluI AGCT 6 cut(s) 102, 228, 279, 421, 534, 592
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 1 cut(s) 324
AoxI GGCC 2 cut(s) 271, 387
ApeKI GCWGC 1 cut(s) 446
AspLEI GCGC 3 cut(s) 429, 438, 584
AspS9I GGNCC 3 cut(s) 125, 272, 394
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 2 cut(s) 125, 394
BanII GRGCYC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 458
BceAI ACGGC 2 cut(s) 292, 298
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 1 cut(s) 356
BfoI RGCGCY 1 cut(s) 430
BfrI CTTAAG 1 cut(s) 629
BisI GCNGC 7 cut(s) 347, 350, 363, 366, 430, 444, 447
BlsI GCNGC 7 cut(s) 348, 351, 364, 367, 431, 445, 448
Bme18I GGWCC 2 cut(s) 125, 394
BmgT120I GGNCC 3 cut(s) 125, 272, 394
BmiI GGNNCC 2 cut(s) 457, 521
BmsI GCATC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 1 cut(s) 224
BsaHI GRCGYC 3 cut(s) 360, 408, 552
BsaJI CCNNGG 3 cut(s) 268, 390, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 385
Bse3DI GCAATG 2 cut(s) 118, 309
BseDI CCNNGG 3 cut(s) 268, 390, 451
BseGI GGATG 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 229, 385
BseMI GCAATG 2 cut(s) 118, 309
BseMII CTCAG 2 cut(s) 11, 215
BseRI GAGGAG 3 cut(s) 281, 431, 530
BseXI GCAGC 1 cut(s) 458
BseYI CCCAGC 1 cut(s) 459
Bsh1236I CGCG 5 cut(s) 245, 384, 405, 582, 584
BshFI GGCC 2 cut(s) 273, 389
BsiHKAI GWGCWC 1 cut(s) 423
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 229, 385
BsmAI GTCTC 1 cut(s) 324
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 2 cut(s) 273, 389
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 2 cut(s) 91, 625
BspANI GGCC 2 cut(s) 273, 389
BspCNI CTCAG 2 cut(s) 12, 216
BspFNI CGCG 5 cut(s) 245, 384, 405, 582, 584
BspLI GGNNCC 2 cut(s) 457, 521
BspTI CTTAAG 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 118, 309
BssECI CCNNGG 3 cut(s) 268, 390, 451
BssMI GATC 2 cut(s) 91, 625
BssNI GRCGYC 3 cut(s) 360, 408, 552
BssT1I CCWWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 619
BstACI GRCGYC 3 cut(s) 360, 408, 552
BstAFI CTTAAG 1 cut(s) 629
BstC8I GCNNGC 4 cut(s) 154, 434, 594, 598
BstDEI CTNAG 2 cut(s) 20, 224
BstDSI CCRYGG 1 cut(s) 451
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 285
BstFNI CGCG 5 cut(s) 245, 384, 405, 582, 584
BstH2I RGCGCY 1 cut(s) 430
BstHHI GCGC 3 cut(s) 429, 438, 584
BstKTI GATC 2 cut(s) 94, 628
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 2 cut(s) 91, 625
BstMWI GCNNNNNNNGC 5 cut(s) 108, 153, 251, 433, 435
BstUI CGCG 5 cut(s) 245, 384, 405, 582, 584
BstV1I GCAGC 1 cut(s) 458
BstXI CCANNNNNNTGG 1 cut(s) 515
BsuRI GGCC 2 cut(s) 273, 389
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 4 cut(s) 154, 434, 594, 598
CfoI GCGC 3 cut(s) 429, 438, 584
Cfr13I GGNCC 3 cut(s) 125, 272, 394
CseI GACGC 4 cut(s) 368, 416, 490, 541
DdeI CTNAG 2 cut(s) 20, 224
DpnI GATC 2 cut(s) 93, 627
DpnII GATC 2 cut(s) 91, 625
Eam1104I CTCTTC 1 cut(s) 619
EarI CTCTTC 1 cut(s) 619
Ecl136II GAGCTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 125, 394
Eco53kI GAGCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 421
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 268
Esp3I CGTCTC 1 cut(s) 324
FaiI YATR 3 cut(s) 40, 195, 549
FaqI GGGAC 1 cut(s) 69
FauI CCCGC 2 cut(s) 307, 351
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 7 cut(s) 347, 350, 363, 366, 430, 444, 447
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 7 cut(s) 347, 350, 363, 366, 430, 444, 447
FspBI CTAG 1 cut(s) 356
GlaI GCGC 3 cut(s) 428, 437, 583
GluI GCNGC 7 cut(s) 347, 350, 363, 366, 430, 444, 447
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 430
HaeIII GGCC 2 cut(s) 273, 389
HgaI GACGC 4 cut(s) 368, 416, 490, 541
HhaI GCGC 3 cut(s) 429, 438, 584
Hin1I GRCGYC 3 cut(s) 360, 408, 552
Hin6I GCGC 3 cut(s) 427, 436, 582
HinP1I GCGC 3 cut(s) 427, 436, 582
HindIII AAGCTT 1 cut(s) 277
HinfI GANTC 5 cut(s) 22, 61, 240, 494, 571
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 21, 84, 373, 557
Hpy188III TCNNGA 2 cut(s) 65, 139
Hpy99I CGWCG 5 cut(s) 313, 319, 410, 494, 506
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 2 cut(s) 147, 152
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 153, 251, 433, 435
HpyF3I CTNAG 2 cut(s) 20, 224
Hsp92I GRCGYC 3 cut(s) 360, 408, 552
HspAI GCGC 3 cut(s) 427, 436, 582
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 625
LmnI GCTCC 3 cut(s) 418, 426, 461
LpnPI CCDG 8 cut(s) 78, 112, 236, 437, 446, 473, 487, 542
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 356
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 93, 627
MboI GATC 2 cut(s) 91, 625
MboII GAAGA 3 cut(s) 566, 569, 606
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 2 cut(s) 46, 142
MlyI GAGTC 2 cut(s) 31, 503
MmeI TCCRAC 1 cut(s) 396
MseI TTAA 2 cut(s) 588, 630
MspCI CTTAAG 1 cut(s) 629
MvnI CGCG 5 cut(s) 245, 384, 405, 582, 584
MwoI GCNNNNNNNGC 5 cut(s) 108, 153, 251, 433, 435
NdeII GATC 2 cut(s) 91, 625
NlaIV GGNNCC 2 cut(s) 457, 521
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 498
PfeI GAWTC 3 cut(s) 61, 240, 571
PkrI GCNGC 7 cut(s) 348, 351, 364, 367, 431, 445, 448
PleI GAGTC 2 cut(s) 30, 502
PpsI GAGTC 2 cut(s) 30, 502
Psp124BI GAGCTC 1 cut(s) 423
PspFI CCCAGC 1 cut(s) 459
PspN4I GGNNCC 2 cut(s) 457, 521
PspPI GGNCC 3 cut(s) 125, 272, 394
PsrI GAACNNNNNNTAC 2 cut(s) 23, 55
SacI GAGCTC 1 cut(s) 423
SaqAI TTAA 2 cut(s) 588, 630
SatI GCNGC 7 cut(s) 347, 350, 363, 366, 430, 444, 447
Sau3AI GATC 2 cut(s) 91, 625
Sau96I GGNCC 3 cut(s) 125, 272, 394
SchI GAGTC 2 cut(s) 31, 503
SduI GDGCHC 1 cut(s) 423
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 2 cut(s) 125, 394
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 2 cut(s) 46, 142
SspMI CTAG 1 cut(s) 356
SstI GAGCTC 1 cut(s) 423
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 175
TaqI TCGA 1 cut(s) 624
TasI AATT 2 cut(s) 46, 142
TauI GCSGC 6 cut(s) 349, 352, 365, 368, 432, 446
TfiI GAWTC 3 cut(s) 61, 240, 571
Tru1I TTAA 2 cut(s) 588, 630
Tru9I TTAA 2 cut(s) 588, 630
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 1 cut(s) 154
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 125, 394
XagI CCTNNNNNAGG 1 cut(s) 383
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.