Rmu_co8510213.1_g000001

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8510213.1
OG0012420
Physical Location & Seq
Forward (+)
1 .. 2908
2908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8510213.1_g000001.1.cds

Sequence Viewer

Length: 1326 bp
cgagttgggattgggattcttcttcgcgcgtttaagctcgctcgcagacaatcctactcttccttgctcgatcttaagcaacttgtctcggaaaaagatctgattgtgagagaagaagcatgccgaattggcttattttttggtggatttagtggttcttaccatgctattagatgcttgttgagaaagtacagaaagaaagagacaccattgaatgcaatcttagcaggttcaattgcggggttgtctattttagcattagatgactcaaaccgaaggcgcacactatctctgtacttgttggcaagggtagcacagtctgcttataattctgcaaagtctaagaacaagtttcacttttggggaagccactggaggcatggcgattctctgctcttttctattgcatgtgcacaggttatgtacgcctttgtaatgcgtcctgagagcttgcctaaatcttatcatgaatttatccagaagactggtcctgttgctcagcctgtatacaaggccgtaagggagtgttgtagaggataccgagtggatattgcctcactggctgattacttggctgagaaagggagaagcgatataaaattggaagagtttccatccattttgccttgttctgttattcatccccacagaaattcttgtttagtgcacaatgcaactgcaacatcagctacatttaggaaaacatttccgctttacttctctttgacgtttgtaccatttgtcgttcttcgcctacagaagttcctggcggcccctgctcgcacctttatgtttgctgttaaagattctgttcgctcaacagcattcttgtctgcttttgttggaatctttcagggtgtgatatgtttgcacagaaaagttgcatcaaaagacaacaagcttgtatattggattgctggtggtctatctgctctttcagttctactggagaaaaaggctaggcgtggtgaacttgctctatatgttcttccacgggcaggagattctttgtggtatattttagtaaaccgtcacctgcttccagatattaagaatgctgaggtgttcttgttctgtttgtgtatgggaggaattatgtactacttggaacatgagccaggcactatggctccattcctgaggggtctgattcgccgctttcttgctagccgaattagtaatccagtcaattcgtctaataggaatgcttcctacacatatttgcaaactcttgatgccatgaagaagccaaagctattagagaatgatggttctgaagcaccttctgagaagaaatacaatcttgaatcaataccggggctgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

441

Amino Acids

49.67

Weight (kDa)

9.83

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AarI CACCTGC 1 cut(s) 1044
Acc36I ACCTGC 2 cut(s) 218, 1044
AccI GTMKAC 1 cut(s) 507
AccII CGCG 2 cut(s) 27, 29
AciI CCGC 4 cut(s) 239, 710, 770, 1156
AcsI RAATTY 2 cut(s) 470, 652
AcuI CTGAAG 1 cut(s) 1296
AfaI GTAC 5 cut(s) 191, 296, 425, 735, 1100
AfiI CCNNNNNNNGG 1 cut(s) 998
AflII CTTAAG 1 cut(s) 74
AgsI TTSAA 3 cut(s) 214, 234, 1307
AjnI CCWGG 2 cut(s) 765, 1117
AluBI AGCT 5 cut(s) 37, 450, 689, 901, 1255
AluI AGCT 5 cut(s) 37, 450, 689, 901, 1255
Alw21I GWGCWC 2 cut(s) 415, 669
Alw26I GTCTC 2 cut(s) 91, 197
Alw44I GTGCAC 2 cut(s) 411, 665
AoxI GGCC 2 cut(s) 513, 771
ApaLI GTGCAC 2 cut(s) 411, 665
ApoI RAATTY 2 cut(s) 470, 652
Asp700I GAANNNNTTC 1 cut(s) 609
AspLEI GCGC 2 cut(s) 29, 282
AspS9I GGNCC 2 cut(s) 488, 772
AsuC2I CCSGG 1 cut(s) 1317
AsuHPI GGTGA 2 cut(s) 980, 1025
AsuNHI GCTAGC 1 cut(s) 1166
AvaII GGWCC 1 cut(s) 488
AxyI CCTNAGG 1 cut(s) 1139
BaeGI GKGCMC 2 cut(s) 415, 669
BaeI ACNNNNGTAYC 2 cut(s) 717, 750
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 415, 669
BbvCI CCTCAGC 1 cut(s) 1059
BccI CCATC 2 cut(s) 622, 1262
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 2 cut(s) 767, 1119
BciVI GTATCC 1 cut(s) 530
BcnI CCSGG 1 cut(s) 1317
BcoDI GTCTC 2 cut(s) 91, 197
BfaI CTAG 2 cut(s) 960, 1167
BfmI CTRYAG 1 cut(s) 755
BfrI CTTAAG 1 cut(s) 74
BfuAI ACCTGC 2 cut(s) 218, 1044
BfuI GTATCC 1 cut(s) 530
BglI GCCNNNNNGGC 2 cut(s) 129, 560
BglII AGATCT 1 cut(s) 97
BisI GCNGC 2 cut(s) 771, 1156
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 2 cut(s) 772, 1157
Bme1390I CCNGG 3 cut(s) 767, 1119, 1317
Bme18I GGWCC 1 cut(s) 488
BmgT120I GGNCC 2 cut(s) 488, 772
BmiI GGNNCC 2 cut(s) 774, 1131
BmrFI CCNGG 3 cut(s) 767, 1119, 1317
BmsI GCATC 3 cut(s) 164, 893, 1225
BmtI GCTAGC 1 cut(s) 1170
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 2 cut(s) 394, 968
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu1102I GCTNAGC 1 cut(s) 498
BpuMI CCSGG 1 cut(s) 1317
BsaJI CCNNGG 2 cut(s) 992, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 1114, 1144
Bsc4I CCNNNNNNNGG 1 cut(s) 998
Bse1I ACTGG 5 cut(s) 377, 490, 564, 951, 1184
Bse21I CCTNAGG 1 cut(s) 1139
BseBI CCWGG 2 cut(s) 767, 1119
BseDI CCNNGG 2 cut(s) 992, 1316
BseGI GGATG 2 cut(s) 614, 640
BseLI CCNNNNNNNGG 1 cut(s) 998
BseMII CTCAG 6 cut(s) 435, 512, 567, 1050, 1130, 1278
BseNI ACTGG 5 cut(s) 377, 490, 564, 951, 1184
BseSI GKGCMC 2 cut(s) 415, 669
Bsh1236I CGCG 2 cut(s) 27, 29
BshFI GGCC 2 cut(s) 515, 773
BsiHKAI GWGCWC 2 cut(s) 415, 669
BsiSI CCGG 1 cut(s) 1316
BslI CCNNNNNNNGG 1 cut(s) 998
BsmAI GTCTC 2 cut(s) 91, 197
BsmI GAATGC 4 cut(s) 220, 824, 1060, 1210
BsnI GGCC 2 cut(s) 515, 773
Bsp1286I GDGCHC 2 cut(s) 415, 669
Bsp143I GATC 2 cut(s) 70, 97
Bsp1720I GCTNAGC 1 cut(s) 498
BspACI CCGC 4 cut(s) 239, 710, 770, 1156
BspANI GGCC 2 cut(s) 515, 773
BspCNI CTCAG 6 cut(s) 436, 511, 568, 1051, 1131, 1279
BspFNI CGCG 2 cut(s) 27, 29
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 774, 1131
BspMI ACCTGC 2 cut(s) 218, 1044
BspOI GCTAGC 1 cut(s) 1170
BspTI CTTAAG 1 cut(s) 74
BsrI ACTGG 5 cut(s) 377, 490, 564, 951, 1184
BssECI CCNNGG 2 cut(s) 992, 1316
BssMI GATC 2 cut(s) 70, 97
BssNAI GTATAC 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 508
Bst2UI CCWGG 2 cut(s) 767, 1119
Bst4CI ACNGT 2 cut(s) 318, 1031
Bst6I CTCTTC 2 cut(s) 64, 600
BstAFI CTTAAG 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 6 cut(s) 39, 43, 121, 452, 781, 1168
BstDEI CTNAG 8 cut(s) 223, 342, 444, 498, 576, 1059, 1139, 1287
BstDSI CCRYGG 1 cut(s) 992
BstF5I GGATG 2 cut(s) 614, 640
BstFNI CGCG 2 cut(s) 27, 29
BstHHI GCGC 2 cut(s) 29, 282
BstKTI GATC 2 cut(s) 73, 100
BstMAI GTCTC 2 cut(s) 91, 197
BstMBI GATC 2 cut(s) 70, 97
BstMWI GCNNNNNNNGC 7 cut(s) 129, 224, 311, 320, 560, 686, 776
BstNI CCWGG 2 cut(s) 767, 1119
BstNSI RCATGY 2 cut(s) 123, 411
BstSCI CCNGG 3 cut(s) 765, 1117, 1315
BstSFI CTRYAG 1 cut(s) 755
BstSLI GKGCMC 2 cut(s) 415, 669
BstUI CGCG 2 cut(s) 27, 29
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 1 cut(s) 97
BstZ17I GTATAC 1 cut(s) 508
Bsu36I CCTNAGG 1 cut(s) 1139
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 2 cut(s) 515, 773
BtgI CCRYGG 1 cut(s) 992
BtsCI GGATG 2 cut(s) 614, 640
BtsIMutI CAGTG 2 cut(s) 370, 557
BveI ACCTGC 2 cut(s) 218, 1044
Cac8I GCNNGC 6 cut(s) 39, 43, 121, 452, 781, 1168
CciI TCATGA 1 cut(s) 466
CfoI GCGC 2 cut(s) 29, 282
Cfr13I GGNCC 2 cut(s) 488, 772
CseI GACGC 1 cut(s) 428
Csp6I GTAC 5 cut(s) 190, 295, 424, 734, 1099
CviAII CATG 7 cut(s) 120, 164, 380, 408, 467, 1112, 1240
CviQI GTAC 5 cut(s) 190, 295, 424, 734, 1099
DdeI CTNAG 8 cut(s) 223, 342, 444, 498, 576, 1059, 1139, 1287
DpnI GATC 2 cut(s) 72, 99
DpnII GATC 2 cut(s) 70, 97
Eam1104I CTCTTC 2 cut(s) 64, 600
EarI CTCTTC 2 cut(s) 64, 600
Eco47I GGWCC 1 cut(s) 488
Eco57I CTGAAG 1 cut(s) 1296
Eco81I CCTNAGG 1 cut(s) 1139
EcoRII CCWGG 2 cut(s) 765, 1117
FaeI CATG 7 cut(s) 123, 167, 383, 411, 470, 1115, 1243
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FatI CATG 7 cut(s) 119, 163, 379, 407, 466, 1111, 1239
FauI CCCGC 1 cut(s) 232
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 2 cut(s) 771, 1156
FokI GGATG 2 cut(s) 601, 627
Fsp4HI GCNGC 2 cut(s) 771, 1156
FspBI CTAG 2 cut(s) 960, 1167
GlaI GCGC 2 cut(s) 28, 281
GluI GCNGC 2 cut(s) 771, 1156
GsuI CTGGAG 2 cut(s) 394, 968
HaeIII GGCC 2 cut(s) 515, 773
HapII CCGG 1 cut(s) 1316
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 29, 282
Hin1II CATG 7 cut(s) 123, 167, 383, 411, 470, 1115, 1243
Hin6I GCGC 2 cut(s) 27, 280
HinP1I GCGC 2 cut(s) 27, 280
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 8 cut(s) 16, 266, 386, 806, 846, 1004, 1150, 1307
HpaII CCGG 1 cut(s) 1316
HphI GGTGA 2 cut(s) 980, 1025
Hpy166II GTNNAC 5 cut(s) 413, 508, 667, 971, 1027
Hpy188I TCNGA 5 cut(s) 91, 102, 1149, 1276, 1288
Hpy188III TCNNGA 7 cut(s) 443, 467, 478, 1043, 1138, 1232, 1304
Hpy8I GTNNAC 5 cut(s) 413, 508, 667, 971, 1027
HpyAV CCTTC 2 cut(s) 270, 1293
HpyCH4III ACNGT 2 cut(s) 318, 1031
HpyCH4IV ACGT 1 cut(s) 728
HpyF10VI GCNNNNNNNGC 7 cut(s) 129, 224, 311, 320, 560, 686, 776
HpyF3I CTNAG 8 cut(s) 223, 342, 444, 498, 576, 1059, 1139, 1287
HpySE526I ACGT 1 cut(s) 728
Hsp92II CATG 7 cut(s) 123, 167, 383, 411, 470, 1115, 1243
HspAI GCGC 2 cut(s) 27, 280
Kzo9I GATC 2 cut(s) 70, 97
LmnI GCTCC 1 cut(s) 1135
LweI GCATC 3 cut(s) 164, 893, 1225
MaeI CTAG 2 cut(s) 960, 1167
MaeII ACGT 1 cut(s) 728
MaeIII GTNAC 1 cut(s) 1031
MalI GATC 2 cut(s) 72, 99
MboI GATC 2 cut(s) 70, 97
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 2 cut(s) 415, 669
MluCI AATT 9 cut(s) 126, 234, 328, 470, 599, 652, 1092, 1173, 1189
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 823
MnlI CCTC 6 cut(s) 369, 527, 565, 1054, 1082, 1134
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 4 cut(s) 33, 75, 801, 1050
MslI CAYNNNNRTG 1 cut(s) 788
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 1 cut(s) 1316
MspR9I CCNGG 3 cut(s) 767, 1119, 1317
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 4 cut(s) 220, 824, 1060, 1210
MvaI CCWGG 2 cut(s) 767, 1119
MvnI CGCG 2 cut(s) 27, 29
MwoI GCNNNNNNNGC 7 cut(s) 129, 224, 311, 320, 560, 686, 776
NciI CCSGG 1 cut(s) 1317
NdeII GATC 2 cut(s) 70, 97
NheI GCTAGC 1 cut(s) 1166
NlaIII CATG 7 cut(s) 123, 167, 383, 411, 470, 1115, 1243
NlaIV GGNNCC 2 cut(s) 774, 1131
NmuCI GTSAC 1 cut(s) 1031
NspI RCATGY 2 cut(s) 123, 411
PaeI GCATGC 1 cut(s) 123
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 1044
PctI GAATGC 4 cut(s) 220, 824, 1060, 1210
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 7 cut(s) 16, 386, 806, 846, 1004, 1150, 1307
PkrI GCNGC 2 cut(s) 772, 1157
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 765, 1117
PspGI CCWGG 2 cut(s) 765, 1117
PspN4I GGNNCC 2 cut(s) 774, 1131
PspPI GGNCC 2 cut(s) 488, 772
PsuI RGATCY 1 cut(s) 97
RsaI GTAC 5 cut(s) 191, 296, 425, 735, 1100
RsaNI GTAC 5 cut(s) 190, 295, 424, 734, 1099
RseI CAYNNNNRTG 1 cut(s) 788
SaqAI TTAA 4 cut(s) 33, 75, 801, 1050
SatI GCNGC 2 cut(s) 771, 1156
Sau3AI GATC 2 cut(s) 70, 97
Sau96I GGNCC 2 cut(s) 488, 772
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 767, 1119, 1317
SduI GDGCHC 2 cut(s) 415, 669
SfaNI GCATC 3 cut(s) 164, 893, 1225
SfcI CTRYAG 1 cut(s) 755
SinI GGWCC 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SphI GCATGC 1 cut(s) 123
Sse9I AATT 9 cut(s) 126, 234, 328, 470, 599, 652, 1092, 1173, 1189
SsiI CCGC 4 cut(s) 239, 710, 770, 1156
SspMI CTAG 2 cut(s) 960, 1167
StyD4I CCNGG 3 cut(s) 765, 1117, 1315
TaaI ACNGT 2 cut(s) 318, 1031
TaiI ACGT 1 cut(s) 731
TaqI TCGA 1 cut(s) 69
TasI AATT 9 cut(s) 126, 234, 328, 470, 599, 652, 1092, 1173, 1189
TatI WGTACW 3 cut(s) 189, 294, 1098
TauI GCSGC 2 cut(s) 773, 1158
TfiI GAWTC 7 cut(s) 16, 386, 806, 846, 1004, 1150, 1307
Tru1I TTAA 4 cut(s) 33, 75, 801, 1050
Tru9I TTAA 4 cut(s) 33, 75, 801, 1050
TscAI CASTG 2 cut(s) 377, 564
TseFI GTSAC 1 cut(s) 1031
Tsp45I GTSAC 1 cut(s) 1031
TspDTI ATGAA 3 cut(s) 483, 629, 1256
TspRI CASTG 2 cut(s) 377, 564
Vha464I CTTAAG 1 cut(s) 74
VneI GTGCAC 2 cut(s) 411, 665
VpaK11BI GGWCC 1 cut(s) 488
XapI RAATTY 2 cut(s) 470, 652
XceI RCATGY 2 cut(s) 123, 411
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 2 cut(s) 960, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.