Prupe.2G326700_v2.0.a1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010247
Physical Location & Seq
Reverse (-)
30133387 .. 30136161
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G326700.1

Sequence Viewer

Length: 1500 bp
ATGACAAGATCCAAATCCCCAGTGCTTCTCTTACTTCTCGCTCTCCTCGCCCTCTCCCTCGTCTTCTTCCTCTTCTCCTTTCATAGCCCCTCCCCCTCCGACGACCCCACCACTCTTCTTCACCCAAACCATCAACCCATTTACAAACCCGAAACCTCCTTCGTCGCCTCCCTCGACCGCTTCCTCCTCGCCCACAAATCACCCCGACGCGATGACACCGTCCTCCTTACAACGCTACCCCAGCACCAACCCAACCAGCTCGACGATCTCATCTTCCAGACCCACACCCAGAGGTTGTACGCTGATCCCTACTACCCGCTCAGCCTGCCCATTAGGGTTTACGTCTACGACATGCCCACCAAATTCACCTACGATCTCCTCTGGCTCTTCAGAAACTCTTACAGGCAGACCTCCAATCTCACCTCTAATGGCAGCCCCGTTCACCGCCTCATCGAACAGCATTCCATTGATTACTGGCTGTGGGCGGATTTGATTGCTCCAGAGTCGGAGAGGCTGTTGAAAAGCGTAGTGAGAGTGCATAGGCAGGAGGAGGCTGACCTCTTCTACATCCCATTCTTCACCACCATTAGCTTCTTCTTATTGGAGAAACAGCAGTGCAAAGCACTTTACAGGGAAGCTCTCAAGTGGGTCACCGATCAGCCTGCCTGGAACCGATCCCAAGGAAGGGATCACATACTTCCGGTTCACCATCCCTGGTCTTTTAAGTCTGTTCGCCGCTTCATGAAGAATGCAATTTGGCTGCTTCCTGATATGGACTCCACCGGAAACTGGTACAAACCTGGACAAGTCTTTCTGGAGAAAGATCTAATTCTTCCTTACGTTCCCAATGTTGATTTTTGTGATTCGAGATGCATATCCGAGACTCAATCCAAGAGAACCACACTGCTCTTTTTCCGCGGCCGGCTTAAAAGAAATGCTGGAGGGAAAATACGCTCAAAACTTGTAGCAGAACTAAGTGGTGCTGAGGGTGTAGCTATAGAGGAGGGGACAGCAGGAGAGGGAGGAAAAGCAGCAGCTCAAGAAGGAATGCGCAAGTCTGTCTTTTGCTTGAGTCCAGCTGGTGATACTCCATCCTCCGCTAGATTGTTCGATGCCATTGTTAGTGGTTGCATACCAGTCATAGTTAGTGATGAACTGGAACTTCCTTTTGAAGGAATACTAGATTATAGAAAGATAGCTTTATTTGTTTCTTCCAGCGATGCCGTACAAACTGGTTGGCTTTTAACATTTCTTAGAAATATTCGCCCTGCTCAGATTGAGGAAATCCGTCAGAATCTTGCCAAGTACTCAAGGCATTTCCTATACTCCAGTCCAGCTCAACCCTTGGGTCCAGAAGACTTGGTTTGGCGAATGATGGCTGGTAAGTTGGTGAATATCAAACTTCACACTCGAAGATCTCAACGTGTGGTGAAAGAGTCGAGAAACATCTGCATGTGTGAATGCAAACGTGCCAACTCTACAACCTCAGGTCACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

500

Amino Acids

57.17

Weight (kDa)

9.1

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1052
AccBSI CCGCTC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 345
AccII CGCG 2 cut(s) 210, 918
AciI CCGC 8 cut(s) 178, 317, 445, 485, 736, 916, 918, 1098
AclWI GGATC 4 cut(s) 3, 299, 669, 696
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 373
AfaI GTAC 4 cut(s) 299, 794, 1227, 1307
AfiI CCNNNNNNNGG 4 cut(s) 684, 685, 789, 1172
AflIII ACRYGT 1 cut(s) 1423
AgsI TTSAA 2 cut(s) 520, 1172
AjnI CCWGG 3 cut(s) 665, 713, 799
AluBI AGCT 8 cut(s) 259, 591, 638, 995, 1037, 1079, 1199, 1337
AluI AGCT 8 cut(s) 259, 591, 638, 995, 1037, 1079, 1199, 1337
Alw26I GTCTC 1 cut(s) 875
AlwI GGATC 4 cut(s) 3, 299, 669, 696
AoxI GGCC 1 cut(s) 919
ApeKI GCWGC 4 cut(s) 432, 760, 1031, 1034
ApoI RAATTY 1 cut(s) 362
AspLEI GCGC 1 cut(s) 1053
AspS9I GGNCC 1 cut(s) 1349
AvaII GGWCC 1 cut(s) 1349
AxyI CCTNAGG 1 cut(s) 1486
BbsI GAAGAC 2 cut(s) 55, 1362
BbvCI CCTCAGC 1 cut(s) 984
BbvI GCAGC 4 cut(s) 444, 747, 1043, 1046
BccI CCATC 4 cut(s) 138, 717, 1099, 1369
BceAI ACGGC 1 cut(s) 1208
BcgI CGANNNNNNTGC 2 cut(s) 644, 678
BciT130I CCWGG 3 cut(s) 667, 715, 801
BcoDI GTCTC 1 cut(s) 875
BfaI CTAG 2 cut(s) 1101, 1181
BfmI CTRYAG 1 cut(s) 996
BglII AGATCT 2 cut(s) 823, 1415
BisI GCNGC 6 cut(s) 433, 736, 761, 919, 1032, 1035
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 6 cut(s) 434, 737, 762, 920, 1033, 1036
BmcAI AGTACT 1 cut(s) 1307
Bme1390I CCNGG 3 cut(s) 667, 715, 801
Bme18I GGWCC 1 cut(s) 1349
BmgT120I GGNCC 1 cut(s) 1349
BmiI GGNNCC 2 cut(s) 671, 1350
BmrFI CCNGG 3 cut(s) 667, 715, 801
BmrI ACTGGG 1 cut(s) 14
BmsI GCATC 3 cut(s) 860, 1102, 1210
BmuI ACTGGG 1 cut(s) 14
BpiI GAAGAC 2 cut(s) 55, 1362
BpmI CTGGAG 4 cut(s) 483, 836, 960, 1312
Bpu10I CCTNAGC 1 cut(s) 984
Bpu1102I GCTNAGC 1 cut(s) 320
BpuEI CTTGAG 4 cut(s) 626, 1023, 1090, 1294
BsaBI GATNNNNATC 2 cut(s) 13, 874
BsaJI CCNNGG 4 cut(s) 679, 713, 916, 1344
BsaWI WCCGGW 2 cut(s) 700, 782
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 4 cut(s) 684, 685, 789, 1172
Bse118I RCCGGY 1 cut(s) 921
Bse1I ACTGG 7 cut(s) 20, 479, 794, 1136, 1161, 1237, 1329
Bse21I CCTNAGG 1 cut(s) 1486
Bse8I GATNNNNATC 2 cut(s) 13, 874
BseBI CCWGG 3 cut(s) 667, 715, 801
BseDI CCNNGG 4 cut(s) 679, 713, 916, 1344
BseGI GGATG 3 cut(s) 567, 709, 1091
BseJI GATNNNNATC 2 cut(s) 13, 874
BseLI CCNNNNNNNGG 4 cut(s) 684, 685, 789, 1172
BseMII CTCAG 4 cut(s) 334, 975, 1286, 1500
BseNI ACTGG 7 cut(s) 20, 479, 794, 1136, 1161, 1237, 1329
BseRI GAGGAG 5 cut(s) 35, 176, 368, 563, 1016
BseX3I CGGCCG 1 cut(s) 919
BseXI GCAGC 4 cut(s) 444, 747, 1043, 1046
BseYI CCCAGC 1 cut(s) 240
Bsh1236I CGCG 2 cut(s) 210, 918
Bsh1285I CGRYCG 2 cut(s) 178, 922
BshFI GGCC 1 cut(s) 921
BsiEI CGRYCG 2 cut(s) 178, 922
BsiSI CCGG 3 cut(s) 701, 783, 922
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 4 cut(s) 684, 685, 789, 1172
BsmAI GTCTC 1 cut(s) 875
BsmFI GGGAC 1 cut(s) 1021
BsmI GAATGC 4 cut(s) 460, 754, 1053, 1466
BsnI GGCC 1 cut(s) 921
Bsp143I GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
Bsp1720I GCTNAGC 1 cut(s) 320
BspACI CCGC 8 cut(s) 178, 317, 445, 485, 736, 916, 918, 1098
BspANI GGCC 1 cut(s) 921
BspCNI CTCAG 4 cut(s) 333, 976, 1285, 1499
BspFNI CGCG 2 cut(s) 210, 918
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 2 cut(s) 671, 1350
BspPI GGATC 4 cut(s) 3, 299, 669, 696
BspQI GCTCTTC 1 cut(s) 392
BsrBI CCGCTC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 921
BsrI ACTGG 7 cut(s) 20, 479, 794, 1136, 1161, 1237, 1329
BssAI RCCGGY 1 cut(s) 921
BssECI CCNNGG 4 cut(s) 679, 713, 916, 1344
BssMI GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
BssT1I CCWWGG 2 cut(s) 679, 1344
Bst2UI CCWGG 3 cut(s) 667, 715, 801
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 77, 120, 392, 566
BstC8I GCNNGC 3 cut(s) 326, 663, 923
BstDEI CTNAG 6 cut(s) 320, 974, 984, 1253, 1272, 1486
BstDSI CCRYGG 1 cut(s) 916
BstEII GGTNACC 1 cut(s) 649
BstENI CCTNNNNNAGG 1 cut(s) 1170
BstF5I GGATG 3 cut(s) 567, 709, 1091
BstFNI CGCG 2 cut(s) 210, 918
BstHHI GCGC 1 cut(s) 1053
BstKTI GATC 9 cut(s) 11, 268, 307, 376, 658, 677, 691, 826, 1418
BstMAI GTCTC 1 cut(s) 875
BstMBI GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
BstMCI CGRYCG 2 cut(s) 178, 922
BstMWI GCNNNNNNNGC 3 cut(s) 47, 241, 325
BstNI CCWGG 3 cut(s) 667, 715, 801
BstNSI RCATGY 2 cut(s) 355, 1456
BstPI GGTNACC 1 cut(s) 649
BstSCI CCNGG 3 cut(s) 665, 713, 799
BstSFI CTRYAG 1 cut(s) 996
BstUI CGCG 2 cut(s) 210, 918
BstV1I GCAGC 4 cut(s) 444, 747, 1043, 1046
BstV2I GAAGAC 2 cut(s) 55, 1362
BstX2I RGATCY 3 cut(s) 8, 823, 1415
BstYI RGATCY 3 cut(s) 8, 823, 1415
BstZI CGGCCG 1 cut(s) 919
Bsu36I CCTNAGG 1 cut(s) 1486
BsuRI GGCC 1 cut(s) 921
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 2 cut(s) 225, 1233
BtsCI GGATG 3 cut(s) 567, 709, 1091
BtsI GCAGTG 2 cut(s) 620, 902
BtsIMutI CAGTG 3 cut(s) 27, 620, 902
Cac8I GCNNGC 3 cut(s) 326, 663, 923
CciI TCATGA 1 cut(s) 741
CfoI GCGC 1 cut(s) 1053
Cfr10I RCCGGY 1 cut(s) 921
Cfr13I GGNCC 1 cut(s) 1349
Cfr42I CCGCGG 1 cut(s) 919
CseI GACGC 1 cut(s) 216
Csp6I GTAC 4 cut(s) 298, 793, 1226, 1306
CviAII CATG 3 cut(s) 352, 742, 1453
CviQI GTAC 4 cut(s) 298, 793, 1226, 1306
DdeI CTNAG 6 cut(s) 320, 974, 984, 1253, 1272, 1486
DpnI GATC 9 cut(s) 10, 267, 306, 375, 657, 676, 690, 825, 1417
DpnII GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
EaeI YGGCCR 1 cut(s) 919
EagI CGGCCG 1 cut(s) 919
Eam1104I CTCTTC 4 cut(s) 77, 120, 392, 566
EarI CTCTTC 4 cut(s) 77, 120, 392, 566
EciI GGCGGA 1 cut(s) 500
EclXI CGGCCG 1 cut(s) 919
Eco130I CCWWGG 2 cut(s) 679, 1344
Eco47I GGWCC 1 cut(s) 1349
Eco52I CGGCCG 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 1486
Eco91I GGTNACC 1 cut(s) 649
EcoNI CCTNNNNNAGG 1 cut(s) 1170
EcoO65I GGTNACC 1 cut(s) 649
EcoRII CCWGG 3 cut(s) 665, 713, 799
EcoT14I CCWWGG 2 cut(s) 679, 1344
EcoT22I ATGCAT 1 cut(s) 875
ErhI CCWWGG 2 cut(s) 679, 1344
FaeI CATG 3 cut(s) 355, 745, 1456
FalI AAGNNNNNCTT 2 cut(s) 1046, 1078
FaqI GGGAC 1 cut(s) 1021
FatI CATG 3 cut(s) 351, 741, 1452
FauI CCCGC 1 cut(s) 324
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 6 cut(s) 433, 736, 761, 919, 1032, 1035
FokI GGATG 3 cut(s) 554, 696, 1078
Fsp4HI GCNGC 6 cut(s) 433, 736, 761, 919, 1032, 1035
FspBI CTAG 2 cut(s) 1101, 1181
FspI TGCGCA 1 cut(s) 1052
GlaI GCGC 1 cut(s) 1052
GluI GCNGC 6 cut(s) 433, 736, 761, 919, 1032, 1035
GsaI CCCAGC 1 cut(s) 244
GsuI CTGGAG 4 cut(s) 483, 836, 960, 1312
HaeIII GGCC 1 cut(s) 921
HapII CCGG 3 cut(s) 701, 783, 922
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 1053
Hin1II CATG 3 cut(s) 355, 745, 1456
Hin6I GCGC 1 cut(s) 1051
HinP1I GCGC 1 cut(s) 1051
HinfI GANTC 7 cut(s) 503, 776, 863, 883, 1072, 1294, 1436
HpaII CCGG 3 cut(s) 701, 783, 922
Hpy166II GTNNAC 4 cut(s) 340, 346, 442, 706
Hpy188I TCNGA 6 cut(s) 100, 392, 508, 880, 1275, 1293
Hpy188III TCNNGA 9 cut(s) 277, 500, 742, 767, 815, 867, 1040, 1352, 1440
Hpy8I GTNNAC 4 cut(s) 340, 346, 442, 706
Hpy99I CGWCG 4 cut(s) 104, 167, 210, 266
HpyAV CCTTC 4 cut(s) 169, 678, 1037, 1166
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 4 cut(s) 342, 840, 1423, 1468
HpyCH4V TGCA 7 cut(s) 538, 618, 752, 873, 1131, 1452, 1464
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 241, 325
HpyF3I CTNAG 6 cut(s) 320, 974, 984, 1253, 1272, 1486
HpySE526I ACGT 4 cut(s) 342, 840, 1423, 1468
Hsp92II CATG 3 cut(s) 355, 745, 1456
HspAI GCGC 1 cut(s) 1051
KroI GCCGGC 1 cut(s) 921
KroNI GCCGGC 1 cut(s) 923
KspI CCGCGG 1 cut(s) 919
Kzo9I GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 502
Lsp1109I GCAGC 4 cut(s) 444, 747, 1043, 1046
LweI GCATC 3 cut(s) 860, 1102, 1210
MaeI CTAG 2 cut(s) 1101, 1181
MaeII ACGT 4 cut(s) 342, 840, 1423, 1468
MaeIII GTNAC 2 cut(s) 649, 1490
MalI GATC 9 cut(s) 10, 267, 306, 375, 657, 676, 690, 825, 1417
MbiI CCGCTC 1 cut(s) 319
MboI GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
MflI RGATCY 3 cut(s) 8, 823, 1415
MluCI AATT 3 cut(s) 362, 753, 828
MlyI GAGTC 5 cut(s) 512, 770, 877, 1081, 1445
MmeI TCCRAC 2 cut(s) 123, 486
Mph1103I ATGCAT 1 cut(s) 875
MroNI GCCGGC 1 cut(s) 921
MseI TTAA 3 cut(s) 723, 927, 1244
MslI CAYNNNNRTG 1 cut(s) 1451
MspA1I CMGCKG 2 cut(s) 918, 1079
MspI CCGG 3 cut(s) 701, 783, 922
MspR9I CCNGG 3 cut(s) 667, 715, 801
Mva1269I GAATGC 4 cut(s) 460, 754, 1053, 1466
MvaI CCWGG 3 cut(s) 667, 715, 801
MvnI CGCG 2 cut(s) 210, 918
MwoI GCNNNNNNNGC 3 cut(s) 47, 241, 325
NaeI GCCGGC 1 cut(s) 923
NdeII GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
NgoMIV GCCGGC 1 cut(s) 921
NlaIII CATG 3 cut(s) 355, 745, 1456
NlaIV GGNNCC 2 cut(s) 671, 1350
NmuCI GTSAC 2 cut(s) 649, 1490
NsbI TGCGCA 1 cut(s) 1052
NsiI ATGCAT 1 cut(s) 875
NspI RCATGY 2 cut(s) 355, 1456
PagI TCATGA 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 4 cut(s) 460, 754, 1053, 1466
PdiI GCCGGC 1 cut(s) 923
PfeI GAWTC 2 cut(s) 863, 1294
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 6 cut(s) 434, 737, 762, 920, 1033, 1036
PleI GAGTC 5 cut(s) 511, 770, 877, 1080, 1444
PpsI GAGTC 5 cut(s) 511, 770, 877, 1080, 1444
Psp6I CCWGG 3 cut(s) 665, 713, 799
PspEI GGTNACC 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 3 cut(s) 665, 713, 799
PspN4I GGNNCC 2 cut(s) 671, 1350
PspPI GGNCC 1 cut(s) 1349
PsuI RGATCY 3 cut(s) 8, 823, 1415
PsyI GACNNNGTC 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 4 cut(s) 299, 794, 1227, 1307
RsaNI GTAC 4 cut(s) 298, 793, 1226, 1306
RseI CAYNNNNRTG 1 cut(s) 1451
SacII CCGCGG 1 cut(s) 919
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 3 cut(s) 723, 927, 1244
SatI GCNGC 6 cut(s) 433, 736, 761, 919, 1032, 1035
Sau3AI GATC 9 cut(s) 8, 265, 304, 373, 655, 674, 688, 823, 1415
Sau96I GGNCC 1 cut(s) 1349
ScaI AGTACT 1 cut(s) 1307
SchI GAGTC 5 cut(s) 512, 770, 877, 1081, 1445
ScrFI CCNGG 3 cut(s) 667, 715, 801
SfaNI GCATC 3 cut(s) 860, 1102, 1210
SfcI CTRYAG 1 cut(s) 996
Sfr303I CCGCGG 1 cut(s) 919
SgrBI CCGCGG 1 cut(s) 919
SinI GGWCC 1 cut(s) 1349
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 4 cut(s) 641, 1038, 1069, 1309
SmoI CTYRAG 4 cut(s) 641, 1038, 1069, 1309
Sse9I AATT 3 cut(s) 362, 753, 828
SsiI CCGC 8 cut(s) 178, 317, 445, 485, 736, 916, 918, 1098
SspI AATATT 1 cut(s) 1261
SspMI CTAG 2 cut(s) 1101, 1181
StyD4I CCNGG 3 cut(s) 665, 713, 799
StyI CCWWGG 2 cut(s) 679, 1344
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 4 cut(s) 345, 843, 1426, 1471
TaqI TCGA 7 cut(s) 174, 261, 453, 866, 1110, 1411, 1439
TasI AATT 3 cut(s) 362, 753, 828
TatI WGTACW 1 cut(s) 1305
TauI GCSGC 2 cut(s) 738, 921
TfiI GAWTC 2 cut(s) 863, 1294
Tru1I TTAA 3 cut(s) 723, 927, 1244
Tru9I TTAA 3 cut(s) 723, 927, 1244
TscAI CASTG 3 cut(s) 27, 620, 909
TseFI GTSAC 2 cut(s) 649, 1490
TseI GCWGC 4 cut(s) 432, 760, 1031, 1034
Tsp45I GTSAC 2 cut(s) 649, 1490
TspDTI ATGAA 4 cut(s) 71, 730, 758, 1167
TspGWI ACGGA 1 cut(s) 1277
TspRI CASTG 3 cut(s) 27, 620, 909
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 1349
XagI CCTNNNNNAGG 1 cut(s) 1170
XapI RAATTY 1 cut(s) 362
XceI RCATGY 2 cut(s) 355, 1456
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 1101, 1181
ZrmI AGTACT 1 cut(s) 1307
Zsp2I ATGCAT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.