Rroxscaffold_4G00276800

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010247
Physical Location & Seq
Forward (+)
282618 .. 286082
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276800.1

Sequence Viewer

Length: 1332 bp
ATGAGAATGGGCGGAAAGCAAATGACAAGATCCAAATCCCCACTTCTAGTATTCACCCTGGTTCTCGCGGCCCTCTCCCTCCTCTTCTTCTTCCTCTTCTCTTTCCGCAGCGACGGCCCCGCCCCATTCTCCGACGGCCCACTCCACCCGAAGCCGATTTACAAACCCGAAACCTCCTTCGTCGCCTCCCTCGAGCGCTTCCTCCTCGCTTTTCACAACTCCCCTCCCTCCGCCGCCCAGAGGACTCACGATCCTAAACACCTCGACGATTTGCTCTTCAACACCGACACTAATCGACTCTATGCAGATCCCTACTACCCACTCACCATGCCTATTAGGGTTTACGTCTACGACATGCCCCCCAAGTTCACCTACGATCTCCTCCGCCTCTTCATCACCTCCTACAAGGACACCTCCAATCTCACCTCCAATGGAAGCCCCGTCCACCGCCTCGTCGAACAGGAAGCTCTCAAGTGGATCACGGATCAGCCTGCCTGGAACCGATCCGGAGGAAGGGATCATATTATTCCAGTTCATCATCCTTGGTCGTTTAAGTCTGTTCGCCGATCTGTGAAAAATGCAATTTGGCTGCTTCCTGACATGGACTCTACAGGCAACTGGTACAAGCCAGGACAAGTTTATTTGGAGAAAGATCTCATTCTTCCTTATGTTGCCAATGTTGATCTTTGCGATGCCAGATGTGTATCTGAGACTCAATCCAGGAGAACAACTCTGCTCTTTTTTCGTGGGCGGCTTAAAAGGAATGCGGGAGGGAAGATACGTGCAAAACTTGTAGCAGAACTTAGTGGGGCTGAGGGTGTATCTATAGAGGAGGGGACAGCTGGAGAAGGAGGAAAGGCAGCAGCTCAGAATGGAATGCGCAAGTCTGTATTTTGCTTAAACCCAGCTGGTGATACTCCATCATCCGCTAGATTATTTGATGCCATTGTTAGTGGTTGCATACCGGTCATAGTTAGTGATGAGTTGGAGCTTCCTTTTGAAGGAATACTAGATTATAGAAAGATTGCTTTATTTGTTTCTTCCAGTGATGCTGTACAACCAGGTTGGCTTTTAACATATCTTAGAAATATTAGCCCTGCTCAAATTGAAGAAATGCGCCAGAATCTTGCTAAGTACTCGAGGCATTTCATATATTCCAGTCCTGCTCAACCCTTGGGTCCGGAAGATTTGGTTTGGAGAATGATGGCTGGTAAGTTGGTGAATATCAAGCTTCACACTCGACGATCTCAACGTGTGGTGAAAGAGTCGAGAAGCATCTGCACCTGCGATTGCAGACGCCCCAATTCCACAACTCCAGGTCACTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

443

Amino Acids

49.87

Weight (kDa)

9.37

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 134 - 360 2.2e-60 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1292
Acc16I TGCGCA 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 1292
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 68
AccIII TCCGGA 2 cut(s) 506, 1180
AclWI GGATC 7 cut(s) 24, 245, 302, 485, 492, 498, 525
AcyI GRCGYC 1 cut(s) 1297
AfaI GTAC 3 cut(s) 623, 1056, 1136
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 3 cut(s) 240, 513, 1001
AflIII ACRYGT 1 cut(s) 1252
AgeI ACCGGT 1 cut(s) 964
AgsI TTSAA 3 cut(s) 280, 1001, 1109
AjnI CCWGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
AluBI AGCT 6 cut(s) 467, 842, 866, 908, 991, 1231
AluI AGCT 6 cut(s) 467, 842, 866, 908, 991, 1231
Alw26I GTCTC 1 cut(s) 704
AlwI GGATC 7 cut(s) 24, 245, 302, 485, 492, 498, 525
Ama87I CYCGRG 2 cut(s) 191, 1138
Aor13HI TCCGGA 2 cut(s) 506, 1180
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 3 cut(s) 69, 115, 136
ApeKI GCWGC 4 cut(s) 108, 589, 860, 863
AsiGI ACCGGT 1 cut(s) 964
AspLEI GCGC 3 cut(s) 198, 882, 1119
AspS9I GGNCC 4 cut(s) 70, 116, 137, 1178
AsuHPI GGTGA 8 cut(s) 46, 316, 361, 388, 415, 923, 1231, 1270
AvaI CYCGRG 2 cut(s) 191, 1138
AvaII GGWCC 1 cut(s) 1178
BarI GAAGNNNNNNTAC 2 cut(s) 143, 175
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 4 cut(s) 120, 576, 872, 875
BccI CCATC 2 cut(s) 928, 1198
BceAI ACGGC 2 cut(s) 130, 151
BciT130I CCWGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
BcoDI GTCTC 1 cut(s) 704
BfaI CTAG 3 cut(s) 47, 930, 1010
BfmI CTRYAG 2 cut(s) 609, 825
BfoI RGCGCY 1 cut(s) 199
BfuAI ACCTGC 1 cut(s) 1292
BglII AGATCT 1 cut(s) 652
BisI GCNGC 7 cut(s) 69, 109, 234, 590, 752, 861, 864
BlsI GCNGC 7 cut(s) 70, 110, 235, 591, 753, 862, 865
BmcAI AGTACT 1 cut(s) 1136
Bme1390I CCNGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
Bme18I GGWCC 1 cut(s) 1178
BmeT110I CYCGRG 2 cut(s) 191, 1138
BmgT120I GGNCC 4 cut(s) 70, 116, 137, 1178
BmiI GGNNCC 3 cut(s) 118, 500, 1179
BmrFI CCNGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
BmsI GCATC 4 cut(s) 682, 931, 1039, 1284
BplI GAGNNNNNCTC 2 cut(s) 715, 747
BpmI CTGGAG 2 cut(s) 864, 1299
Bpu10I CCTNAGC 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 455
BsaAI YACGTR 1 cut(s) 782
BsaBI GATNNNNATC 2 cut(s) 34, 703
BsaHI GRCGYC 1 cut(s) 1297
BsaJI CCNNGG 3 cut(s) 57, 542, 1173
BsaWI WCCGGW 3 cut(s) 506, 964, 1180
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 513, 1001
Bse118I RCCGGY 1 cut(s) 964
Bse1I ACTGG 4 cut(s) 530, 623, 1044, 1158
Bse8I GATNNNNATC 2 cut(s) 34, 703
BseAI TCCGGA 2 cut(s) 506, 1180
BseBI CCWGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
BseDI CCNNGG 3 cut(s) 57, 542, 1173
BseGI GGATG 2 cut(s) 538, 923
BseJI GATNNNNATC 2 cut(s) 34, 703
BseLI CCNNNNNNNGG 3 cut(s) 240, 513, 1001
BseMII CTCAG 3 cut(s) 699, 804, 881
BseNI ACTGG 4 cut(s) 530, 623, 1044, 1158
BseRI GAGGAG 4 cut(s) 71, 194, 371, 845
BseXI GCAGC 4 cut(s) 120, 576, 872, 875
BseYI CCCAGC 1 cut(s) 904
BsgI GTGCAG 1 cut(s) 1264
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 3 cut(s) 71, 117, 138
BshTI ACCGGT 1 cut(s) 964
BsiHKCI CYCGRG 2 cut(s) 191, 1138
BsiSI CCGG 3 cut(s) 507, 965, 1181
BslFI GGGAC 1 cut(s) 850
BslI CCNNNNNNNGG 3 cut(s) 240, 513, 1001
BsmAI GTCTC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 850
BsmI GAATGC 2 cut(s) 769, 882
BsnI GGCC 3 cut(s) 71, 117, 138
BsoBI CYCGRG 2 cut(s) 191, 1138
Bsp13I TCCGGA 2 cut(s) 506, 1180
Bsp1407I TGTACA 1 cut(s) 1054
BspANI GGCC 3 cut(s) 71, 117, 138
BspCNI CTCAG 3 cut(s) 700, 805, 880
BspEI TCCGGA 2 cut(s) 506, 1180
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 118, 500, 1179
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 7 cut(s) 24, 245, 302, 485, 492, 498, 525
BspQI GCTCTTC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 964
BsrGI TGTACA 1 cut(s) 1054
BsrI ACTGG 4 cut(s) 530, 623, 1044, 1158
BssAI RCCGGY 1 cut(s) 964
BssECI CCNNGG 3 cut(s) 57, 542, 1173
BssNI GRCGYC 1 cut(s) 1297
BssT1I CCWWGG 2 cut(s) 542, 1173
Bst2UI CCWGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
Bst6I CTCTTC 4 cut(s) 89, 101, 281, 395
BstACI GRCGYC 1 cut(s) 1297
BstAUI TGTACA 1 cut(s) 1054
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 6 cut(s) 708, 803, 813, 867, 1082, 1131
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 2 cut(s) 538, 923
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 3 cut(s) 198, 882, 1119
BstMAI GTCTC 1 cut(s) 704
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
BstSFI CTRYAG 2 cut(s) 609, 825
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 4 cut(s) 120, 576, 872, 875
BstX2I RGATCY 3 cut(s) 29, 307, 652
BstYI RGATCY 3 cut(s) 29, 307, 652
BsuRI GGCC 3 cut(s) 71, 117, 138
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 2 cut(s) 538, 923
BtsIMutI CAGTG 1 cut(s) 1051
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 3 cut(s) 198, 882, 1119
Cfr10I RCCGGY 1 cut(s) 964
Cfr13I GGNCC 4 cut(s) 70, 116, 137, 1178
CseI GACGC 1 cut(s) 1305
CsiI ACCWGGT 1 cut(s) 1060
Csp6I GTAC 3 cut(s) 622, 1055, 1135
CspAI ACCGGT 1 cut(s) 964
CviAII CATG 3 cut(s) 328, 355, 601
CviQI GTAC 3 cut(s) 622, 1055, 1135
DdeI CTNAG 6 cut(s) 708, 803, 813, 867, 1082, 1131
Eam1104I CTCTTC 4 cut(s) 89, 101, 281, 395
EarI CTCTTC 4 cut(s) 89, 101, 281, 395
EciI GGCGGA 3 cut(s) 27, 220, 374
Eco130I CCWWGG 2 cut(s) 542, 1173
Eco47I GGWCC 1 cut(s) 1178
Eco47III AGCGCT 1 cut(s) 197
Eco88I CYCGRG 2 cut(s) 191, 1138
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
EcoT14I CCWWGG 2 cut(s) 542, 1173
ErhI CCWWGG 2 cut(s) 542, 1173
FaeI CATG 3 cut(s) 331, 358, 604
FaqI GGGAC 1 cut(s) 850
FatI CATG 3 cut(s) 327, 354, 600
FauI CCCGC 2 cut(s) 127, 760
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 7 cut(s) 69, 109, 234, 590, 752, 861, 864
FokI GGATG 2 cut(s) 525, 910
Fsp4HI GCNGC 7 cut(s) 69, 109, 234, 590, 752, 861, 864
FspBI CTAG 3 cut(s) 47, 930, 1010
FspI TGCGCA 1 cut(s) 881
GlaI GCGC 3 cut(s) 197, 881, 1118
GluI GCNGC 7 cut(s) 69, 109, 234, 590, 752, 861, 864
GsaI CCCAGC 1 cut(s) 908
GsuI CTGGAG 2 cut(s) 864, 1299
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 3 cut(s) 71, 117, 138
HapII CCGG 3 cut(s) 507, 965, 1181
HgaI GACGC 1 cut(s) 1305
HhaI GCGC 3 cut(s) 198, 882, 1119
Hin1I GRCGYC 1 cut(s) 1297
Hin1II CATG 3 cut(s) 331, 358, 604
Hin6I GCGC 3 cut(s) 196, 880, 1117
HinP1I GCGC 3 cut(s) 196, 880, 1117
HindIII AAGCTT 1 cut(s) 1229
HinfI GANTC 6 cut(s) 244, 297, 605, 712, 1123, 1265
HpaII CCGG 3 cut(s) 507, 965, 1181
HphI GGTGA 8 cut(s) 46, 316, 361, 388, 415, 923, 1231, 1270
Hpy166II GTNNAC 4 cut(s) 343, 349, 369, 445
Hpy188I TCNGA 3 cut(s) 133, 709, 870
Hpy188III TCNNGA 5 cut(s) 248, 507, 596, 1181, 1269
Hpy8I GTNNAC 4 cut(s) 343, 349, 369, 445
Hpy99I CGWCG 6 cut(s) 116, 137, 185, 269, 458, 1245
HpyAV CCTTC 4 cut(s) 187, 507, 842, 995
HpyCH4IV ACGT 3 cut(s) 345, 781, 1252
HpyCH4V TGCA 6 cut(s) 305, 581, 785, 960, 1281, 1293
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 6 cut(s) 708, 803, 813, 867, 1082, 1131
HpySE526I ACGT 3 cut(s) 345, 781, 1252
Hsp92I GRCGYC 1 cut(s) 1297
Hsp92II CATG 3 cut(s) 331, 358, 604
HspAI GCGC 3 cut(s) 196, 880, 1117
Kpn2I TCCGGA 2 cut(s) 506, 1180
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 4 cut(s) 120, 576, 872, 875
LweI GCATC 4 cut(s) 682, 931, 1039, 1284
MabI ACCWGGT 1 cut(s) 1060
MaeI CTAG 3 cut(s) 47, 930, 1010
MaeII ACGT 3 cut(s) 345, 781, 1252
MaeIII GTNAC 1 cut(s) 1319
MflI RGATCY 3 cut(s) 29, 307, 652
MluCI AATT 3 cut(s) 582, 1104, 1303
MlyI GAGTC 5 cut(s) 238, 291, 599, 706, 1274
MmeI TCCRAC 2 cut(s) 156, 966
MroI TCCGGA 2 cut(s) 506, 1180
MseI TTAA 4 cut(s) 552, 756, 899, 1073
MspA1I CMGCKG 2 cut(s) 842, 908
MspI CCGG 3 cut(s) 507, 965, 1181
MspR9I CCNGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
Mva1269I GAATGC 2 cut(s) 769, 882
MvaI CCWGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 114
NlaIII CATG 3 cut(s) 331, 358, 604
NlaIV GGNNCC 3 cut(s) 118, 500, 1179
NmuCI GTSAC 1 cut(s) 1319
NsbI TGCGCA 1 cut(s) 881
NspI RCATGY 1 cut(s) 358
PaeR7I CTCGAG 2 cut(s) 191, 1138
PaqCI CACCTGC 1 cut(s) 1292
PciSI GCTCTTC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 1249
PctI GAATGC 2 cut(s) 769, 882
PfeI GAWTC 1 cut(s) 1123
PfoI TCCNGGA 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 964
PkrI GCNGC 7 cut(s) 70, 110, 235, 591, 753, 862, 865
PleI GAGTC 5 cut(s) 238, 291, 599, 706, 1273
PpsI GAGTC 5 cut(s) 238, 291, 599, 706, 1273
Ppu21I YACGTR 1 cut(s) 782
Psp6I CCWGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
PspFI CCCAGC 1 cut(s) 904
PspGI CCWGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
PspN4I GGNNCC 3 cut(s) 118, 500, 1179
PspPI GGNCC 4 cut(s) 70, 116, 137, 1178
PspXI VCTCGAGB 2 cut(s) 191, 1138
PsuI RGATCY 3 cut(s) 29, 307, 652
PvuII CAGCTG 2 cut(s) 842, 908
RsaI GTAC 3 cut(s) 623, 1056, 1136
RsaNI GTAC 3 cut(s) 622, 1055, 1135
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 4 cut(s) 552, 756, 899, 1073
SatI GCNGC 7 cut(s) 69, 109, 234, 590, 752, 861, 864
Sau96I GGNCC 4 cut(s) 70, 116, 137, 1178
ScaI AGTACT 1 cut(s) 1136
SchI GAGTC 5 cut(s) 238, 291, 599, 706, 1274
ScrFI CCNGG 6 cut(s) 59, 496, 630, 721, 1062, 1317
SexAI ACCWGGT 1 cut(s) 1060
SfaNI GCATC 4 cut(s) 682, 931, 1039, 1284
SfcI CTRYAG 2 cut(s) 609, 825
Sfr274I CTCGAG 2 cut(s) 191, 1138
SinI GGWCC 1 cut(s) 1178
SlaI CTCGAG 2 cut(s) 191, 1138
SmlI CTYRAG 3 cut(s) 191, 470, 1138
SmoI CTYRAG 3 cut(s) 191, 470, 1138
Sse9I AATT 3 cut(s) 582, 1104, 1303
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 47, 930, 1010
StyD4I CCNGG 6 cut(s) 57, 494, 628, 719, 1060, 1315
StyI CCWWGG 2 cut(s) 542, 1173
TaiI ACGT 3 cut(s) 348, 784, 1255
TaqI TCGA 7 cut(s) 192, 264, 295, 456, 1139, 1240, 1268
TasI AATT 3 cut(s) 582, 1104, 1303
TatI WGTACW 2 cut(s) 1054, 1134
TauI GCSGC 3 cut(s) 71, 236, 754
TfiI GAWTC 1 cut(s) 1123
Tru1I TTAA 4 cut(s) 552, 756, 899, 1073
Tru9I TTAA 4 cut(s) 552, 756, 899, 1073
TscAI CASTG 1 cut(s) 1051
TseFI GTSAC 1 cut(s) 1319
TseI GCWGC 4 cut(s) 108, 589, 860, 863
Tsp45I GTSAC 1 cut(s) 1319
TspDTI ATGAA 3 cut(s) 382, 524, 1138
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 1 cut(s) 1051
VpaK11BI GGWCC 1 cut(s) 1178
XagI CCTNNNNNAGG 1 cut(s) 999
XceI RCATGY 1 cut(s) 358
XhoI CTCGAG 2 cut(s) 191, 1138
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 3 cut(s) 47, 930, 1010
ZrmI AGTACT 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.