Prupe.3G020600_v2.0.a1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013494
Physical Location & Seq
Forward (+)
1501836 .. 1506795
4960 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G020600.1

Sequence Viewer

Length: 1392 bp
ATGGCAGAAACCAGTACTAGCAGTAGCAATAGTTTCAATAACACCAATGGGGGCTCACCAATTTCCTACATGGCCCAAGACCACCTCTTTACAATCTTGTTGCTTCTTCCTGTAGATTCAATTCTTTGCTTTGCCATGGCCTGCAAGAAGTTTAGAGCTTTGACAGCCTCTGACACTCTGTGGGAGTCCATATGCAGAAGGGACTGGGGTCCCAAATCAGTTGAGGCCCTCAAGGCTTCAAATTTTCATCATCAGCTGCCTTGGATGAGGCTCTACAAGCAAGTCTCTCGCTTGGACTCTGTTTATTGCCATAAATTGTCTAACCCAGATGGGGAATTGGTGTTTCCAAGTCCCAGGGCCTCTCATTCTCTCAATTTTGTGTCTGATTGCTTGGTTTTGTTTGGTGGTGGCAGTGAGGGAGGACGCCATCTAGATGACACATGGGTGGCATACGTTGGTAATGATTTTCGGAGAATGTTGAAGTGGCAGAAGATCAATTCAGGCATTCCAAGTGGCAGATTTGGGCATTCATGTGTTGTTATTGATGATTTTCTTGTTCTATTTGGAGGAATAAATGACAATGGAAGCCGTCACAACGATACATGGGTGGGGAAAGTAGCATGCCATGAGACCCTTGGTATCACACTGTCATGGAGGCTGCTTGTCGTGGGGCCCGATGCACCTTCGCAGCGAGGGGCTCATGCTGCATGCTGCATTGATGGCAGAAAGATGGTTATCCATGGAGGAATTGGGCTGCATGGTGTCAGGTTGGGTGATACATGGGTGTTGGAACTCTCCGAAAATTTTCGATTTGGAACTTGGTATGAAATCGTGGCTCATCCATGTCCGCCAGCTCGCTCAGGACACACATTGACTTGCATTGGGGGAACCCGAACAGTTTTATTTGGAGGGAGAGGATTGGGCTATGATGTGCTTCATGATATCTGGTTCTTGGATATTCATGAAGGTCAACCTAAATGGGTACAAATACTCTATGAATTACAAAATGTTCCTGGAGGAGTTTCCCTCCCTAGAGTTGGTCACTCAGCTACTCTCATCTTAGGAGGTCATTTGCTGATTTGTGGAGGAGAGGATTCGTACAGACACCGGAAGAATGACTTCTGGGTTTTAGACATAAGTGCAGTTCCATCCATTACAATGCAGCCAACTACACTCAACTCTGTGCAGTTATTGGCAAAAATGTGGAAAAGGTTGAAGGCAAACGGTTACAAACCCAAATGCCGGTCATTCCATCGTGCCTGCACAGATAATTCCGGGCGTTACTTGTTTGTGTTTGGTGGAATGGTGGATGGCGTACTTCAGCCTGCTGACCCTGCCGGGCTGAGGTTTGATGGAGAGCTCGTCCTTGTGGAGCTTGTGCTCCAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_3 Kelch_4 Kelch_5

Protein Analysis

464

Amino Acids

51.19

Weight (kDa)

8.17

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51550
fragaria_vesca FvH4_6g37110
malus_domestica MD09G1161500.v1.1 MD17G1147400.v1.1
prunus_persica Prupe.3G020600_v2.0.a1
pyrus_communis pycom09g08000 pycom09g08050 pycom17g14000
rosa_chinensis RchiOBHm_Chr2g0149671
rosa_laevigata RLG00000020436
rosa_multiflora Rmu_sc0014307.1_g000005
rosa_roxburghii Rroxscaffold_2G00097810
rosa_rugosa Rorug02G0414300
rosa_samantha Rh2AG474500 Rh2BG486200 Rh2CG460200 Rh2DG495500
rosa_wichuraiana Rw2G038690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 848
AcsI RAATTY 2 cut(s) 241, 802
AcuI CTGAAG 1 cut(s) 1304
AcyI GRCGYC 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 180
AfaI GTAC 4 cut(s) 16, 984, 1100, 1317
AfiI CCNNNNNNNGG 4 cut(s) 331, 1037, 1242, 1344
AgsI TTSAA 5 cut(s) 37, 120, 240, 481, 1216
AjnI CCWGG 2 cut(s) 353, 1012
AleI CACNNNNGTG 1 cut(s) 443
AluBI AGCT 7 cut(s) 158, 256, 854, 1049, 1360, 1375, 1387
AluI AGCT 7 cut(s) 158, 256, 854, 1049, 1360, 1375, 1387
Alw21I GWGCWC 2 cut(s) 1362, 1383
Alw26I GTCTC 2 cut(s) 289, 623
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 5 cut(s) 72, 138, 225, 357, 671
ApaI GGGCCC 1 cut(s) 675
ApeKI GCWGC 7 cut(s) 256, 658, 688, 704, 711, 754, 1162
ApoI RAATTY 2 cut(s) 241, 802
Asp700I GAANNNNTTC 2 cut(s) 804, 1118
AspS9I GGNCC 6 cut(s) 73, 209, 226, 357, 671, 672
AsuC2I CCSGG 2 cut(s) 1276, 1339
AsuHPI GGTGA 2 cut(s) 48, 785
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 675
BaeI ACNNNNGTAYC 4 cut(s) 591, 624, 768, 801
BanII GRGCYC 4 cut(s) 56, 675, 700, 1362
Bbv12I GWGCWC 2 cut(s) 1362, 1383
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 7 cut(s) 243, 645, 691, 698, 700, 741, 1174
BccI CCATC 8 cut(s) 323, 435, 713, 724, 1156, 1260, 1304, 1346
BceAI ACGGC 1 cut(s) 573
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 2 cut(s) 355, 1014
BcnI CCSGG 2 cut(s) 1276, 1339
BcoDI GTCTC 2 cut(s) 289, 623
BfaI CTAG 3 cut(s) 18, 431, 1032
BfmI CTRYAG 1 cut(s) 111
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 7 cut(s) 257, 659, 689, 705, 712, 755, 1163
BlsI GCNGC 7 cut(s) 258, 660, 690, 706, 713, 756, 1164
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 4 cut(s) 355, 1014, 1276, 1339
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 6 cut(s) 73, 209, 226, 357, 671, 672
BmiI GGNNCC 5 cut(s) 210, 211, 672, 673, 889
BmrFI CCNGG 4 cut(s) 355, 1014, 1276, 1339
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 1011, 1043
BpmI CTGGAG 2 cut(s) 1035, 1367
Bpu10I CCTNAGC 2 cut(s) 859, 1343
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 1276, 1339
BsaBI GATNNNNATC 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 6 cut(s) 135, 260, 353, 354, 634, 739
BsaWI WCCGGW 1 cut(s) 1107
BsaXI ACNNNNNCTCC 2 cut(s) 1347, 1377
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 1037, 1242, 1344
Bse118I RCCGGY 1 cut(s) 1242
Bse1I ACTGG 2 cut(s) 12, 209
Bse8I GATNNNNATC 1 cut(s) 734
BseBI CCWGG 2 cut(s) 355, 1014
BseDI CCNNGG 6 cut(s) 135, 260, 353, 354, 634, 739
BseGI GGATG 4 cut(s) 270, 838, 1148, 1315
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 4 cut(s) 331, 1037, 1242, 1344
BseMII CTCAG 3 cut(s) 873, 1059, 1334
BseNI ACTGG 2 cut(s) 12, 209
BseRI GAGGAG 2 cut(s) 1032, 1101
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 7 cut(s) 243, 645, 691, 698, 700, 741, 1174
BsgI GTGCAG 3 cut(s) 1161, 1205, 1246
BshFI GGCC 5 cut(s) 74, 140, 227, 359, 673
BsiHKAI GWGCWC 2 cut(s) 1362, 1383
BsiSI CCGG 4 cut(s) 1108, 1243, 1275, 1338
BslFI GGGAC 3 cut(s) 195, 215, 336
BslI CCNNNNNNNGG 4 cut(s) 331, 1037, 1242, 1344
BsmAI GTCTC 2 cut(s) 289, 623
BsmFI GGGAC 3 cut(s) 195, 215, 336
BsmI GAATGC 2 cut(s) 504, 526
BsnI GGCC 5 cut(s) 74, 140, 227, 359, 673
Bso31I GGTCTC 1 cut(s) 623
Bsp120I GGGCCC 1 cut(s) 671
Bsp1286I GDGCHC 5 cut(s) 56, 675, 700, 1362, 1383
Bsp143I GATC 1 cut(s) 492
Bsp19I CCATGG 2 cut(s) 135, 739
BspACI CCGC 1 cut(s) 848
BspANI GGCC 5 cut(s) 74, 140, 227, 359, 673
BspCNI CTCAG 3 cut(s) 872, 1058, 1335
BspHI TCATGA 2 cut(s) 937, 961
BspLI GGNNCC 5 cut(s) 210, 211, 672, 673, 889
BspTNI GGTCTC 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 1242
BsrI ACTGG 2 cut(s) 12, 209
BssAI RCCGGY 1 cut(s) 1242
BssECI CCNNGG 6 cut(s) 135, 260, 353, 354, 634, 739
BssMI GATC 1 cut(s) 492
BssNI GRCGYC 1 cut(s) 424
BssT1I CCWWGG 4 cut(s) 135, 260, 634, 739
Bst2UI CCWGG 2 cut(s) 355, 1014
Bst4CI ACNGT 3 cut(s) 648, 898, 1226
BstACI GRCGYC 1 cut(s) 424
BstC8I GCNNGC 7 cut(s) 142, 622, 709, 852, 856, 1261, 1326
BstDEI CTNAG 4 cut(s) 859, 1045, 1060, 1343
BstDSI CCRYGG 2 cut(s) 135, 739
BstF5I GGATG 4 cut(s) 270, 838, 1148, 1315
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 2 cut(s) 289, 623
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 6 cut(s) 164, 233, 277, 704, 720, 1334
BstNI CCWGG 2 cut(s) 355, 1014
BstNSI RCATGY 2 cut(s) 624, 711
BstPAI GACNNNNGTC 1 cut(s) 207
BstSCI CCNGG 4 cut(s) 353, 1012, 1274, 1337
BstSFI CTRYAG 1 cut(s) 111
BstSLI GKGCMC 1 cut(s) 675
BstV1I GCAGC 7 cut(s) 243, 645, 691, 698, 700, 741, 1174
BsuRI GGCC 5 cut(s) 74, 140, 227, 359, 673
BtgI CCRYGG 2 cut(s) 135, 739
BtsCI GGATG 4 cut(s) 270, 838, 1148, 1315
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 418, 644
Cac8I GCNNGC 7 cut(s) 142, 622, 709, 852, 856, 1261, 1326
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 2 cut(s) 937, 961
Cfr10I RCCGGY 1 cut(s) 1242
Cfr13I GGNCC 6 cut(s) 73, 209, 226, 357, 671, 672
CseI GACGC 1 cut(s) 432
Csp6I GTAC 4 cut(s) 15, 983, 1099, 1316
CviQI GTAC 4 cut(s) 15, 983, 1099, 1316
DdeI CTNAG 4 cut(s) 859, 1045, 1060, 1343
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
DraIII CACNNNGTG 1 cut(s) 180
EciI GGCGGA 1 cut(s) 837
Ecl136II GAGCTC 1 cut(s) 1360
Eco130I CCWWGG 4 cut(s) 135, 260, 634, 739
Eco24I GRGCYC 4 cut(s) 56, 675, 700, 1362
Eco31I GGTCTC 1 cut(s) 623
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 1360
Eco57I CTGAAG 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 1360
EcoO109I RGGNCCY 4 cut(s) 209, 226, 357, 671
EcoRII CCWGG 2 cut(s) 353, 1012
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 4 cut(s) 135, 260, 634, 739
EcoT38I GRGCYC 4 cut(s) 56, 675, 700, 1362
ErhI CCWWGG 4 cut(s) 135, 260, 634, 739
FalI AAGNNNNNCTT 2 cut(s) 1103, 1135
FaqI GGGAC 3 cut(s) 195, 215, 336
FauNDI CATATG 1 cut(s) 191
Fnu4HI GCNGC 7 cut(s) 257, 659, 689, 705, 712, 755, 1163
FokI GGATG 4 cut(s) 277, 825, 1135, 1322
FriOI GRGCYC 4 cut(s) 56, 675, 700, 1362
Fsp4HI GCNGC 7 cut(s) 257, 659, 689, 705, 712, 755, 1163
FspBI CTAG 3 cut(s) 18, 431, 1032
GluI GCNGC 7 cut(s) 257, 659, 689, 705, 712, 755, 1163
GsuI CTGGAG 2 cut(s) 1035, 1367
HaeIII GGCC 5 cut(s) 74, 140, 227, 359, 673
HapII CCGG 4 cut(s) 1108, 1243, 1275, 1338
HgaI GACGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 424
HincII GTYRAC 1 cut(s) 971
HindII GTYRAC 1 cut(s) 971
HinfI GANTC 4 cut(s) 116, 185, 296, 1094
HpaII CCGG 4 cut(s) 1108, 1243, 1275, 1338
HphI GGTGA 2 cut(s) 48, 785
Hpy166II GTNNAC 1 cut(s) 971
Hpy188I TCNGA 4 cut(s) 172, 385, 471, 799
Hpy188III TCNNGA 4 cut(s) 431, 861, 938, 962
Hpy8I GTNNAC 1 cut(s) 971
HpyAV CCTTC 4 cut(s) 192, 693, 959, 1210
HpyCH4III ACNGT 3 cut(s) 648, 898, 1226
HpyCH4IV ACGT 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 233, 277, 704, 720, 1334
HpyF3I CTNAG 4 cut(s) 859, 1045, 1060, 1343
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 424
KflI GGGWCCC 1 cut(s) 209
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 1372, 1386
Lsp1109I GCAGC 7 cut(s) 243, 645, 691, 698, 700, 741, 1174
LweI GCATC 1 cut(s) 667
MaeI CTAG 3 cut(s) 18, 431, 1032
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 4 cut(s) 590, 1040, 1226, 1280
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 3 cut(s) 98, 502, 1123
MhlI GDGCHC 5 cut(s) 56, 675, 700, 1362, 1383
MlyI GAGTC 2 cut(s) 194, 290
MmeI TCCRAC 1 cut(s) 768
MroXI GAANNNNTTC 2 cut(s) 804, 1118
MslI CAYNNNNRTG 5 cut(s) 432, 443, 531, 649, 1157
MspA1I CMGCKG 2 cut(s) 256, 1387
MspI CCGG 4 cut(s) 1108, 1243, 1275, 1338
MspR9I CCNGG 4 cut(s) 355, 1014, 1276, 1339
Mva1269I GAATGC 2 cut(s) 504, 526
MvaI CCWGG 2 cut(s) 355, 1014
MwoI GCNNNNNNNGC 6 cut(s) 164, 233, 277, 704, 720, 1334
NciI CCSGG 2 cut(s) 1276, 1339
NcoI CCATGG 2 cut(s) 135, 739
NdeI CATATG 1 cut(s) 191
NdeII GATC 1 cut(s) 492
NlaIV GGNNCC 5 cut(s) 210, 211, 672, 673, 889
NmuCI GTSAC 2 cut(s) 590, 1040
NspI RCATGY 2 cut(s) 624, 711
OliI CACNNNNGTG 1 cut(s) 443
PaeI GCATGC 2 cut(s) 624, 711
PagI TCATGA 2 cut(s) 937, 961
PasI CCCWGGG 1 cut(s) 354
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 2 cut(s) 504, 526
PdmI GAANNNNTTC 2 cut(s) 804, 1118
PfeI GAWTC 2 cut(s) 116, 1094
PfoI TCCNGGA 1 cut(s) 1012
PkrI GCNGC 7 cut(s) 258, 660, 690, 706, 713, 756, 1164
PleI GAGTC 2 cut(s) 193, 290
PpsI GAGTC 2 cut(s) 193, 290
PpuMI RGGWCCY 1 cut(s) 209
PshAI GACNNNNGTC 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 1362
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 353, 1012
PspGI CCWGG 2 cut(s) 353, 1012
PspN4I GGNNCC 5 cut(s) 210, 211, 672, 673, 889
PspOMI GGGCCC 1 cut(s) 671
PspPI GGNCC 6 cut(s) 73, 209, 226, 357, 671, 672
PspPPI RGGWCCY 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 170
PvuII CAGCTG 2 cut(s) 256, 1387
RsaI GTAC 4 cut(s) 16, 984, 1100, 1317
RsaNI GTAC 4 cut(s) 15, 983, 1099, 1316
RseI CAYNNNNRTG 5 cut(s) 432, 443, 531, 649, 1157
SacI GAGCTC 1 cut(s) 1362
SatI GCNGC 7 cut(s) 257, 659, 689, 705, 712, 755, 1163
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 6 cut(s) 73, 209, 226, 357, 671, 672
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 2 cut(s) 194, 290
ScrFI CCNGG 4 cut(s) 355, 1014, 1276, 1339
SduI GDGCHC 5 cut(s) 56, 675, 700, 1362, 1383
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 5 cut(s) 432, 443, 531, 649, 1157
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SphI GCATGC 2 cut(s) 624, 711
SsiI CCGC 1 cut(s) 848
SspMI CTAG 3 cut(s) 18, 431, 1032
SstI GAGCTC 1 cut(s) 1362
StyD4I CCNGG 4 cut(s) 353, 1012, 1274, 1337
StyI CCWWGG 4 cut(s) 135, 260, 634, 739
TaaI ACNGT 3 cut(s) 648, 898, 1226
TaiI ACGT 1 cut(s) 456
TaqI TCGA 1 cut(s) 808
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 2 cut(s) 116, 1094
TscAI CASTG 2 cut(s) 418, 651
TseFI GTSAC 2 cut(s) 590, 1040
TseI GCWGC 7 cut(s) 256, 658, 688, 704, 711, 754, 1162
Tsp45I GTSAC 2 cut(s) 590, 1040
TspDTI ATGAA 7 cut(s) 236, 519, 840, 926, 950, 978, 1011
TspRI CASTG 2 cut(s) 418, 651
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 2 cut(s) 241, 802
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 2 cut(s) 624, 711
XcmI CCANNNNNNNNNTGG 2 cut(s) 632, 746
XmnI GAANNNNTTC 2 cut(s) 804, 1118
XspI CTAG 3 cut(s) 18, 431, 1032
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.