pycom09g08000

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013494
Physical Location & Seq
Forward (+)
6135897 .. 6138822
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08000.1

Sequence Viewer

Length: 1398 bp
ATGGCAGAAACCAGCACTAGCAGCAGAAGCAGCAGCTTCGGTTACTCCTCCAGTGGCTCACCAATTTCCAACTTAGCCCAAGACCACCTCTTTACAATCCTGCTGCGCCTCCCTGCGGATTCAATTCTTTGTTTTGCCATGACCTGCAAAAAGTTCAGAGCTTTGGCAGCATCTGACAGTCTGTGGGAGTCCATATGCAGGAGGGACTGGGGACCCAAATCAGTAGAGGCCCTCAAAGCTTCAAACTTTGGCCTACAGCAGCTGCCATGGATGAGGCTCTACAAGCAAGTCTCTAGGGTTGATTCTGTTTATTGCCGTAAACTTTCTGACCCAGATGGGGGATTGGGATTTCCATGTCCCAGGGCCTCTCATTCCCTAAATTTTGTGTCTGGTTGCTTGGTGTTGTTTGGTGGTGGATGTGAGGGAGGACGCCATCTAGACGACACATGGGTGGCATACATTGGTAACGATTTTCGGAGAATGTTGAAGTGGCAGAAGATAAATTCTGGCATTCCAAGTGGTAGGTTCGGGCATTCGTGTGTTGTAATTGATGATTTTCTTGTTATGTTTGGAGGAATAAATGACAATGGGGGCCGTCACAATGATACATGGGTGGGGCAAGTGGCATGCCATGAGAGTCTTGGTGTCACACTCTCATGGAAGCTGCTTGTTGTGGGATCCGACGCACCTTCCCAGCGAGGGGCTCTTGCTGCGTGCTGCATTGACGGCAGAAAGATGGTTATACATGGAGGAATTGGGCTCCACGGTGTGAGATTGTGTGACACATGGGTGTTGGAACTCTCAGAAAATTTTCGGTTTGGAACTTGGTACGAAATCGGGACTCATCCATGTCCCCCAGCTCGTTCGGGACACACACTGACCTGCATTGGAGGAACACGAACGGTTTTATTTGGTGGGAGAGGATTACGTTACGACGTGCTTAATGATGTCTGGTTCTTGGACATTTGTGATGATAATCCGAAATGGGTACAAGTACGCTATGAGCTACAAAATGTTCCAGGAGGAGTTTCTCTTCCACGAGTTGGTCACTCAGCCACGCTCATGTTAGGCGGCCATCTACTGATCTGTGGGGGAGAGGATTCGTACAGACACCGGAAAAATGACTTCTGGATTTTAGACATAAATGCAGCTCCATCCATTACAATGCAGCAGCCAACTACGCTGAACTCCATGCAGGTATCGGTAAAAAGGTGGAAGAGGTTGAAGGCAAATGGTTACAAGCCCAAATGCCGGTCATTCCATCGAGCCTGCACAGATAACTCGGGGCGGTACTTGTTTGTGTTTGGCGGAATGATGGATGGCTTGATTCAGCCGGCAGACCCTGCCGGGCTGAGGTTTGGCAGCGAGCTCCTGCTTGTGGAGCTTGTGCTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_3 Kelch_4 Kelch_5

Protein Analysis

466

Amino Acids

51.3

Weight (kDa)

8.83

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 63 4.9e-06 F-box domain
F-box-like PF12937 30 - 68 1.4e-06 F-box-like
Kelch_KLHDC2_KLHL20_DRC7 PF24681 117 - 269 2.2e-22 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 117 - 270 5.7e-22 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 163 - 251 2.9e-07 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 163 - 439 1.1e-10 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 217 - 382 7.3e-25 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 247 - 439 4.8e-09 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 275 - 382 1.6e-15 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 285 - 439 9.6e-09 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 394 - 439 9.3e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51550
fragaria_vesca FvH4_6g37110
malus_domestica MD09G1161500.v1.1 MD17G1147400.v1.1
prunus_persica Prupe.3G020600_v2.0.a1
pyrus_communis pycom09g08000 pycom09g08050 pycom17g14000
rosa_chinensis RchiOBHm_Chr2g0149671
rosa_laevigata RLG00000020436
rosa_multiflora Rmu_sc0014307.1_g000005
rosa_roxburghii Rroxscaffold_2G00097810
rosa_rugosa Rorug02G0414300
rosa_samantha Rh2AG474500 Rh2BG486200 Rh2CG460200 Rh2DG495500
rosa_wichuraiana Rw2G038690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 152, 890, 1186
AccB7I CCANNNNNTGG 1 cut(s) 1043
AciI CCGC 4 cut(s) 116, 1071, 1288, 1308
AclWI GGATC 2 cut(s) 672, 685
AcoI YGGCCR 1 cut(s) 1072
AcsI RAATTY 3 cut(s) 379, 502, 808
AcyI GRCGYC 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 769
AfaI GTAC 5 cut(s) 830, 990, 996, 1106, 1292
AgsI TTSAA 4 cut(s) 123, 243, 487, 1225
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 2 cut(s) 359, 1018
AleI CACNNNNGTG 2 cut(s) 449, 788
Alw21I GWGCWC 1 cut(s) 1371
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 2 cut(s) 672, 685
AlwNI CAGNNNCTG 3 cut(s) 173, 262, 1343
Ama87I CYCGRG 1 cut(s) 1282
AoxI GGCC 5 cut(s) 228, 250, 363, 592, 1072
ApoI RAATTY 3 cut(s) 379, 502, 808
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 4 cut(s) 212, 229, 363, 592
AsuC2I CCSGG 1 cut(s) 1348
AsuHPI GGTGA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 1282
AvaII GGWCC 1 cut(s) 212
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BamHI GGATCC 1 cut(s) 677
BanII GRGCYC 3 cut(s) 706, 762, 1371
BauI CACGAG 1 cut(s) 1038
Bbv12I GWGCWC 1 cut(s) 1371
BbvCI CCTCAGC 2 cut(s) 1352, 1391
BccI CCATC 8 cut(s) 329, 441, 730, 1083, 1162, 1269, 1309, 1313
BceAI ACGGC 3 cut(s) 300, 579, 742
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 2 cut(s) 361, 1020
BcnI CCSGG 1 cut(s) 1348
BcoDI GTCTC 1 cut(s) 295
BfaI CTAG 3 cut(s) 18, 294, 437
BfmI CTRYAG 1 cut(s) 254
BfuAI ACCTGC 3 cut(s) 152, 890, 1186
Bme1390I CCNGG 3 cut(s) 361, 1020, 1348
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 1282
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 4 cut(s) 212, 229, 363, 592
BmiI GGNNCC 5 cut(s) 213, 214, 593, 679, 761
BmrFI CCNGG 3 cut(s) 361, 1020, 1348
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 34
Bpu10I CCTNAGC 2 cut(s) 1352, 1391
BpuMI CCSGG 1 cut(s) 1348
BsaBI GATNNNNATC 1 cut(s) 975
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 4 cut(s) 266, 359, 360, 763
BsaWI WCCGGW 1 cut(s) 1113
Bse118I RCCGGY 2 cut(s) 1251, 1333
Bse1I ACTGG 2 cut(s) 51, 212
Bse8I GATNNNNATC 1 cut(s) 975
BseBI CCWGG 2 cut(s) 361, 1020
BseDI CCNNGG 4 cut(s) 266, 359, 360, 763
BseGI GGATG 5 cut(s) 276, 422, 844, 1154, 1324
BseJI GATNNNNATC 1 cut(s) 975
BseMII CTCAG 4 cut(s) 816, 1065, 1343, 1382
BseNI ACTGG 2 cut(s) 51, 212
BseRI GAGGAG 2 cut(s) 37, 1038
BseYI CCCAGC 2 cut(s) 693, 856
BsgI GTGCAG 1 cut(s) 1255
BshFI GGCC 5 cut(s) 230, 252, 365, 594, 1074
BsiHKAI GWGCWC 1 cut(s) 1371
BsiHKCI CYCGRG 1 cut(s) 1282
BsiSI CCGG 4 cut(s) 1114, 1252, 1334, 1347
BslFI GGGAC 6 cut(s) 218, 225, 342, 837, 853, 882
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 6 cut(s) 218, 225, 342, 837, 853, 882
BsmI GAATGC 2 cut(s) 510, 532
BsnI GGCC 5 cut(s) 230, 252, 365, 594, 1074
BsoBI CYCGRG 1 cut(s) 1282
Bsp1286I GDGCHC 3 cut(s) 706, 762, 1371
Bsp143I GATC 2 cut(s) 677, 1083
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 4 cut(s) 116, 1071, 1288, 1308
BspANI GGCC 5 cut(s) 230, 252, 365, 594, 1074
BspCNI CTCAG 4 cut(s) 815, 1064, 1344, 1383
BspLI GGNNCC 5 cut(s) 213, 214, 593, 679, 761
BspMI ACCTGC 3 cut(s) 152, 890, 1186
BspPI GGATC 2 cut(s) 672, 685
BsrFI RCCGGY 2 cut(s) 1251, 1333
BsrI ACTGG 2 cut(s) 51, 212
BssAI RCCGGY 2 cut(s) 1251, 1333
BssECI CCNNGG 4 cut(s) 266, 359, 360, 763
BssMI GATC 2 cut(s) 677, 1083
BssNI GRCGYC 1 cut(s) 430
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 1 cut(s) 266
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 2 cut(s) 361, 1020
Bst4CI ACNGT 3 cut(s) 179, 767, 904
Bst6I CTCTTC 2 cut(s) 1038, 1211
BstACI GRCGYC 1 cut(s) 430
BstAPI GCANNNNNTGC 1 cut(s) 1343
BstC8I GCNNGC 5 cut(s) 628, 715, 1270, 1335, 1367
BstDEI CTNAG 5 cut(s) 73, 802, 1051, 1352, 1391
BstDSI CCRYGG 2 cut(s) 266, 763
BstF5I GGATG 5 cut(s) 276, 422, 844, 1154, 1324
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 680, 1086
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 2 cut(s) 677, 1083
BstNI CCWGG 2 cut(s) 361, 1020
BstNSI RCATGY 1 cut(s) 630
BstSCI CCNGG 3 cut(s) 359, 1018, 1346
BstSFI CTRYAG 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 677
BstYI RGATCY 1 cut(s) 677
BsuRI GGCC 5 cut(s) 230, 252, 365, 594, 1074
BtgI CCRYGG 2 cut(s) 266, 763
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 5 cut(s) 276, 422, 844, 1154, 1324
BtsIMutI CAGTG 2 cut(s) 58, 875
BveI ACCTGC 3 cut(s) 152, 890, 1186
Cac8I GCNNGC 5 cut(s) 628, 715, 1270, 1335, 1367
CaiI CAGNNNCTG 3 cut(s) 173, 262, 1343
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 2 cut(s) 1251, 1333
Cfr13I GGNCC 4 cut(s) 212, 229, 363, 592
CseI GACGC 2 cut(s) 438, 692
Csp6I GTAC 5 cut(s) 829, 989, 995, 1105, 1291
CviQI GTAC 5 cut(s) 829, 989, 995, 1105, 1291
DdeI CTNAG 5 cut(s) 73, 802, 1051, 1352, 1391
DpnI GATC 2 cut(s) 679, 1085
DpnII GATC 2 cut(s) 677, 1083
DraIII CACNNNGTG 1 cut(s) 769
EaeI YGGCCR 1 cut(s) 1072
Eam1104I CTCTTC 2 cut(s) 1038, 1211
EarI CTCTTC 2 cut(s) 1038, 1211
EciI GGCGGA 1 cut(s) 1323
Ecl136II GAGCTC 1 cut(s) 1369
Eco130I CCWWGG 1 cut(s) 266
Eco24I GRGCYC 3 cut(s) 706, 762, 1371
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 1369
Eco88I CYCGRG 1 cut(s) 1282
EcoICRI GAGCTC 1 cut(s) 1369
EcoO109I RGGNCCY 3 cut(s) 212, 229, 363
EcoRII CCWGG 2 cut(s) 359, 1018
EcoT14I CCWWGG 1 cut(s) 266
EcoT38I GRGCYC 3 cut(s) 706, 762, 1371
ErhI CCWWGG 1 cut(s) 266
FaqI GGGAC 6 cut(s) 218, 225, 342, 837, 853, 882
FauNDI CATATG 1 cut(s) 194
FokI GGATG 5 cut(s) 283, 429, 831, 1141, 1331
FriOI GRGCYC 3 cut(s) 706, 762, 1371
FspBI CTAG 3 cut(s) 18, 294, 437
GlaI GCGC 1 cut(s) 107
GsaI CCCAGC 2 cut(s) 697, 860
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 5 cut(s) 230, 252, 365, 594, 1074
HapII CCGG 4 cut(s) 1114, 1252, 1334, 1347
HgaI GACGC 2 cut(s) 438, 692
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 430
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 7 cut(s) 119, 188, 302, 637, 841, 1100, 1327
HpaII CCGG 4 cut(s) 1114, 1252, 1334, 1347
HphI GGTGA 1 cut(s) 51
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 7 cut(s) 158, 175, 328, 477, 682, 805, 981
Hpy188III TCNNGA 4 cut(s) 437, 838, 867, 1129
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 2 cut(s) 686, 938
HpyAV CCTTC 2 cut(s) 699, 1219
HpyCH4III ACNGT 3 cut(s) 179, 767, 904
HpyCH4IV ACGT 2 cut(s) 928, 936
HpyCH4V TGCA 8 cut(s) 147, 198, 720, 885, 1148, 1168, 1195, 1272
HpyF3I CTNAG 5 cut(s) 73, 802, 1051, 1352, 1391
HpySE526I ACGT 2 cut(s) 928, 936
Hsp92I GRCGYC 1 cut(s) 430
HspAI GCGC 1 cut(s) 106
KflI GGGWCCC 1 cut(s) 212
KroI GCCGGC 1 cut(s) 1333
KroNI GCCGGC 1 cut(s) 1335
Kzo9I GATC 2 cut(s) 677, 1083
LmnI GCTCC 4 cut(s) 765, 1156, 1374, 1381
LweI GCATC 1 cut(s) 179
MaeI CTAG 3 cut(s) 18, 294, 437
MaeII ACGT 2 cut(s) 928, 936
MaeIII GTNAC 8 cut(s) 41, 464, 596, 646, 779, 929, 1046, 1235
MalI GATC 2 cut(s) 679, 1085
MboI GATC 2 cut(s) 677, 1083
MboII GAAGA 3 cut(s) 508, 1025, 1228
MflI RGATCY 1 cut(s) 677
MhlI GDGCHC 3 cut(s) 706, 762, 1371
MluCI AATT 7 cut(s) 63, 123, 379, 502, 546, 753, 808
MlyI GAGTC 3 cut(s) 197, 646, 835
MmeI TCCRAC 3 cut(s) 93, 705, 774
MroNI GCCGGC 1 cut(s) 1333
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 1 cut(s) 942
MslI CAYNNNNRTG 6 cut(s) 449, 537, 655, 788, 1061, 1163
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 4 cut(s) 1114, 1252, 1334, 1347
MspR9I CCNGG 3 cut(s) 361, 1020, 1348
Mva1269I GAATGC 2 cut(s) 510, 532
MvaI CCWGG 2 cut(s) 361, 1020
NaeI GCCGGC 1 cut(s) 1335
NciI CCSGG 1 cut(s) 1348
NcoI CCATGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 194
NdeII GATC 2 cut(s) 677, 1083
NgoMIV GCCGGC 1 cut(s) 1333
NlaIV GGNNCC 5 cut(s) 213, 214, 593, 679, 761
NmuCI GTSAC 4 cut(s) 596, 646, 779, 1046
NspI RCATGY 1 cut(s) 630
OliI CACNNNNGTG 2 cut(s) 449, 788
PaeI GCATGC 1 cut(s) 630
PasI CCCWGGG 1 cut(s) 360
PctI GAATGC 2 cut(s) 510, 532
PdiI GCCGGC 1 cut(s) 1335
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 4 cut(s) 119, 302, 1100, 1327
PflMI CCANNNNNTGG 1 cut(s) 1043
PfoI TCCNGGA 1 cut(s) 1018
PleI GAGTC 3 cut(s) 196, 645, 835
PpsI GAGTC 3 cut(s) 196, 645, 835
PpuMI RGGWCCY 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 1371
Psp5II RGGWCCY 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 359, 1018
PspFI CCCAGC 2 cut(s) 693, 856
PspGI CCWGG 2 cut(s) 359, 1018
PspN4I GGNNCC 5 cut(s) 213, 214, 593, 679, 761
PspPI GGNCC 4 cut(s) 212, 229, 363, 592
PspPPI RGGWCCY 1 cut(s) 212
PstNI CAGNNNCTG 3 cut(s) 173, 262, 1343
PsuI RGATCY 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 5 cut(s) 830, 990, 996, 1106, 1292
RsaNI GTAC 5 cut(s) 829, 989, 995, 1105, 1291
RseI CAYNNNNRTG 6 cut(s) 449, 537, 655, 788, 1061, 1163
SacI GAGCTC 1 cut(s) 1371
SaqAI TTAA 1 cut(s) 942
Sau3AI GATC 2 cut(s) 677, 1083
Sau96I GGNCC 4 cut(s) 212, 229, 363, 592
SchI GAGTC 3 cut(s) 197, 646, 835
ScrFI CCNGG 3 cut(s) 361, 1020, 1348
SduI GDGCHC 3 cut(s) 706, 762, 1371
SfaNI GCATC 1 cut(s) 179
SfcI CTRYAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 6 cut(s) 449, 537, 655, 788, 1061, 1163
SphI GCATGC 1 cut(s) 630
Sse9I AATT 7 cut(s) 63, 123, 379, 502, 546, 753, 808
SsiI CCGC 4 cut(s) 116, 1071, 1288, 1308
SspMI CTAG 3 cut(s) 18, 294, 437
SstI GAGCTC 1 cut(s) 1371
StyD4I CCNGG 3 cut(s) 359, 1018, 1346
StyI CCWWGG 1 cut(s) 266
TaaI ACNGT 3 cut(s) 179, 767, 904
TaiI ACGT 2 cut(s) 931, 939
TaqI TCGA 1 cut(s) 1264
TasI AATT 7 cut(s) 63, 123, 379, 502, 546, 753, 808
TauI GCSGC 1 cut(s) 1074
TfiI GAWTC 4 cut(s) 119, 302, 1100, 1327
Tru1I TTAA 1 cut(s) 942
Tru9I TTAA 1 cut(s) 942
TscAI CASTG 2 cut(s) 58, 882
TseFI GTSAC 4 cut(s) 596, 646, 779, 1046
Tsp45I GTSAC 4 cut(s) 596, 646, 779, 1046
TspRI CASTG 2 cut(s) 58, 882
Van91I CCANNNNNTGG 1 cut(s) 1043
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 3 cut(s) 379, 502, 808
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 1 cut(s) 630
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 3 cut(s) 18, 294, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.