Rh2BG486200

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013494
Physical Location & Seq
Reverse (-)
68622273 .. 68625828
3556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG486200.1

Sequence Viewer

Length: 1377 bp
ATGGCAGAAACCAGTACCAGCAGCAGTTGCAATGGCTCTCCATTGACCCAAATAGCTCAAGACCACCTCTTCACAATCTTGCTACTCCTCCCTGTTGATTCTATTCTCAGCTTTGCCATGACTTGCAAGAAGTTTAGAGCTCTCACAGCTTCTGATACTCTGTGGGAGTACATATGCCAGAGGGACTGGGGCACCAAATCAGTTGAGGCCATCAAGGCTTCAAATTCTAATCAGCAGTTGCCTTGGATGAGGATTTACAAGCAAGTTTCTGGGTTTGACTCTGTGTATTGCCATAGAGTGTGTGCTGATCCAGATGCTGAGCTGGTGTTTCCAACTCCCAGGGCCTCTCATTCCCTCAATTTTGTGTCTGATTGCTTGGTTTTGTTTGGTGGTGGCTGTGAGGGAGGACGCCATCTAGATGACACATGGGTGGCATACATGGGTAATGATTTTCAGAGATTGCTGAAGTGGCAGAGGATCAGTTCGGGCAGTCCAAGTGGAAGATTTGGGCATACATGTGCTGTAATTGATGATTTTCTTGTTCTATTTGGAGGTATAAATGACAATGGAAACCGCCAAAACGATACATGGGTGGGGCAAGTAGCATGCCATGATAGCCTTGGTATCACATTGTCCTGGAGGCTGCTTGTGGTGGGGGACAATGCACCTCCCCAGCGAGGAGCTCATGCGGCATGCTGCATTGAAGGTAGAAAGATGGTTATCCATGGAGGAATTGGGCGGTTTGGTGTGAGACTGAGTGACACATGGGTATTGGAACTCTCAGAAAACTTTCAAACTGGTACTTGGTATGAAATCGTTATGCAGCCATCTCCCCCAGCTCGCTCAGGTCACACGTTGACTTGCATTGGGGGAACCCGAACTGTTTTATTTGGAGGAAGAGGATTGGGATATGATGTGCTTCATGATCTTTGGCTCTTGGATATTTGTGAGGGTCATCCACAATGGTTACAAGTACTATACGAGTTACAAAATATTCCTGGAGGAGTTTCCCTTCCACGGGTTGGTCACTCGGCTACACTCATCTTAGGAGGTCGTTTACTGATATGTGGAGGGGAAGACTCGTATAGACACCGGAAGAATGACTTCTGGGTTTTAGACATTACTGCAGTTCCCTCCATTACGGTGCAGCCAACTATGTTGAACTCTATGCATTTATTTGCAAAAATGTGGAAAAGATTGAAGACAAAGGGTCATCTACCCAACTGCCGGTCATTTCATCGTGCCTGTGCAGATCAATCTGGGCGTTGCGTGTTTATGTTTGGCGGAATGGTGGATGGTTTACTTCAACCTGCTGACCCAGCTGGGCTGAGGTTTGATGGAGAGCTCCTCCTTGTGGAGCTTGTGCTTAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_3 Kelch_4 Kelch_5

Protein Analysis

458

Amino Acids

50.61

Weight (kDa)

6.75

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 61 1e-05 F-box-like
Beta-prop_FBX42 PF13415 106 - 263 2e-23 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 107 - 262 7.7e-24 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 156 - 246 4.4e-08 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 156 - 432 3.2e-12 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 170 - 375 2e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 263 - 375 1.9e-14 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 263 - 431 1.9e-07 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 278 - 375 2.6e-08 tRNA wybutosine-synthesizing protein 4, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51550
fragaria_vesca FvH4_6g37110
malus_domestica MD09G1161500.v1.1 MD17G1147400.v1.1
prunus_persica Prupe.3G020600_v2.0.a1
pyrus_communis pycom09g08000 pycom09g08050 pycom17g14000
rosa_chinensis RchiOBHm_Chr2g0149671
rosa_laevigata RLG00000020436
rosa_multiflora Rmu_sc0014307.1_g000005
rosa_roxburghii Rroxscaffold_2G00097810
rosa_rugosa Rorug02G0414300
rosa_samantha Rh2AG474500 Rh2BG486200 Rh2CG460200 Rh2DG495500
rosa_wichuraiana Rw2G038690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1318
AccB1I GGYRCC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 1022
AciI CCGC 4 cut(s) 574, 689, 739, 1284
AclWI GGATC 2 cut(s) 302, 485
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 4 cut(s) 16, 170, 802, 975
AfiI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 2 cut(s) 515, 852
AgsI TTSAA 6 cut(s) 222, 704, 794, 1162, 1201, 1307
AhdI GACNNNNNGTC 1 cut(s) 1209
AjnI CCWGG 3 cut(s) 338, 635, 997
AleI CACNNNNGTG 1 cut(s) 428
Alw21I GWGCWC 3 cut(s) 142, 685, 1347
Alw26I GTCTC 1 cut(s) 745
AlwI GGATC 2 cut(s) 302, 485
AlwNI CAGNNNCTG 2 cut(s) 152, 317
AoxI GGCC 2 cut(s) 207, 342
ApeKI GCWGC 5 cut(s) 21, 643, 696, 823, 1147
ApoI RAATTY 1 cut(s) 223
Asp700I GAANNNNTTC 2 cut(s) 789, 1103
AspS9I GGNCC 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanI GGYRCC 1 cut(s) 191
BanII GRGCYC 3 cut(s) 142, 685, 1347
BbsI GAAGAC 2 cut(s) 1083, 1208
Bbv12I GWGCWC 3 cut(s) 142, 685, 1347
BbvCI CCTCAGC 1 cut(s) 1328
BbvI GCAGC 5 cut(s) 33, 630, 683, 835, 1159
BccI CCATC 6 cut(s) 218, 420, 709, 835, 1289, 1331
BciT130I CCWGG 3 cut(s) 340, 637, 999
BcoDI GTCTC 1 cut(s) 745
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 1125
BfrI CTTAAG 1 cut(s) 1367
BfuAI ACCTGC 1 cut(s) 1318
BglI GCCNNNNNGGC 1 cut(s) 215
BisI GCNGC 6 cut(s) 22, 644, 690, 697, 824, 1148
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 6 cut(s) 23, 645, 691, 698, 825, 1149
BmcAI AGTACT 1 cut(s) 975
Bme1390I CCNGG 3 cut(s) 340, 637, 999
BmeRI GACNNNNNGTC 1 cut(s) 1209
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 2 cut(s) 193, 874
BmrFI CCNGG 3 cut(s) 340, 637, 999
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 1083, 1208
BplI GAGNNNNNCTC 2 cut(s) 1332, 1364
BpmI CTGGAG 2 cut(s) 658, 1020
Bpu10I CCTNAGC 2 cut(s) 844, 1328
Bpu1102I GCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BsaWI WCCGGW 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
Bse118I RCCGGY 1 cut(s) 1227
Bse1I ACTGG 3 cut(s) 12, 191, 802
Bse3DI GCAATG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 3 cut(s) 340, 637, 999
BseDI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BseGI GGATG 3 cut(s) 252, 955, 1300
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 6 cut(s) 121, 309, 746, 795, 858, 1319
BseNI ACTGG 3 cut(s) 12, 191, 802
BseRI GAGGAG 4 cut(s) 77, 693, 1017, 1337
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 5 cut(s) 33, 630, 683, 835, 1159
BseYI CCCAGC 4 cut(s) 672, 835, 1318, 1322
BsgI GTGCAG 2 cut(s) 1166, 1269
BshFI GGCC 2 cut(s) 209, 344
BshNI GGYRCC 1 cut(s) 191
BsiHKAI GWGCWC 3 cut(s) 142, 685, 1347
BsiSI CCGG 2 cut(s) 1093, 1228
BslFI GGGAC 2 cut(s) 197, 671
BslI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
BsmAI GTCTC 1 cut(s) 745
BsmFI GGGAC 2 cut(s) 197, 671
BsnI GGCC 2 cut(s) 209, 344
Bsp1286I GDGCHC 4 cut(s) 142, 194, 685, 1347
Bsp143I GATC 4 cut(s) 307, 477, 925, 1252
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 4 cut(s) 574, 689, 739, 1284
BspANI GGCC 2 cut(s) 209, 344
BspCNI CTCAG 6 cut(s) 120, 310, 747, 794, 857, 1320
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 2 cut(s) 193, 874
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 1 cut(s) 1318
BspPI GGATC 2 cut(s) 302, 485
BspT107I GGYRCC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 1367
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 1227
BsrI ACTGG 3 cut(s) 12, 191, 802
BssAI RCCGGY 1 cut(s) 1227
BssECI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BssMI GATC 4 cut(s) 307, 477, 925, 1252
BssNI GRCGYC 1 cut(s) 409
BssT1I CCWWGG 3 cut(s) 242, 619, 724
Bst2UI CCWGG 3 cut(s) 340, 637, 999
Bst4CI ACNGT 2 cut(s) 883, 1144
Bst6I CTCTTC 2 cut(s) 74, 892
BstACI GRCGYC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 1367
BstAPI GCANNNNNTGC 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 607, 694, 841
BstDEI CTNAG 7 cut(s) 107, 318, 755, 781, 844, 1045, 1328
BstDSI CCRYGG 2 cut(s) 724, 1016
BstF5I GGATG 3 cut(s) 252, 955, 1300
BstKTI GATC 4 cut(s) 310, 480, 928, 1255
BstMAI GTCTC 1 cut(s) 745
BstMBI GATC 4 cut(s) 307, 477, 925, 1252
BstMWI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
BstNI CCWGG 3 cut(s) 340, 637, 999
BstNSI RCATGY 3 cut(s) 519, 609, 696
BstSCI CCNGG 3 cut(s) 338, 635, 997
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 5 cut(s) 33, 630, 683, 835, 1159
BstV2I GAAGAC 2 cut(s) 1083, 1208
BsuRI GGCC 2 cut(s) 209, 344
BtgI CCRYGG 2 cut(s) 724, 1016
BtsCI GGATG 3 cut(s) 252, 955, 1300
BveI ACCTGC 1 cut(s) 1318
Cac8I GCNNGC 3 cut(s) 607, 694, 841
CaiI CAGNNNCTG 2 cut(s) 152, 317
CciI TCATGA 1 cut(s) 922
Cfr10I RCCGGY 1 cut(s) 1227
Cfr13I GGNCC 1 cut(s) 342
CseI GACGC 1 cut(s) 417
Csp6I GTAC 4 cut(s) 15, 169, 801, 974
CviQI GTAC 4 cut(s) 15, 169, 801, 974
DdeI CTNAG 7 cut(s) 107, 318, 755, 781, 844, 1045, 1328
DpnI GATC 4 cut(s) 309, 479, 927, 1254
DpnII GATC 4 cut(s) 307, 477, 925, 1252
DriI GACNNNNNGTC 1 cut(s) 1209
Eam1104I CTCTTC 2 cut(s) 74, 892
Eam1105I GACNNNNNGTC 1 cut(s) 1209
EarI CTCTTC 2 cut(s) 74, 892
EciI GGCGGA 1 cut(s) 1299
Ecl136II GAGCTC 3 cut(s) 140, 683, 1345
Eco130I CCWWGG 3 cut(s) 242, 619, 724
Eco24I GRGCYC 3 cut(s) 142, 685, 1347
Eco53kI GAGCTC 3 cut(s) 140, 683, 1345
Eco57I CTGAAG 1 cut(s) 485
EcoICRI GAGCTC 3 cut(s) 140, 683, 1345
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 3 cut(s) 338, 635, 997
EcoT14I CCWWGG 3 cut(s) 242, 619, 724
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 3 cut(s) 142, 685, 1347
ErhI CCWWGG 3 cut(s) 242, 619, 724
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 2 cut(s) 197, 671
FauNDI CATATG 1 cut(s) 173
Fnu4HI GCNGC 6 cut(s) 22, 644, 690, 697, 824, 1148
FokI GGATG 3 cut(s) 259, 942, 1307
FriOI GRGCYC 3 cut(s) 142, 685, 1347
Fsp4HI GCNGC 6 cut(s) 22, 644, 690, 697, 824, 1148
FspBI CTAG 1 cut(s) 416
GluI GCNGC 6 cut(s) 22, 644, 690, 697, 824, 1148
GsaI CCCAGC 4 cut(s) 676, 839, 1322, 1326
GsuI CTGGAG 2 cut(s) 658, 1020
HaeIII GGCC 2 cut(s) 209, 344
HapII CCGG 2 cut(s) 1093, 1228
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 409
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 3 cut(s) 98, 278, 1079
HpaII CCGG 2 cut(s) 1093, 1228
Hpy166II GTNNAC 3 cut(s) 858, 1058, 1301
Hpy188I TCNGA 4 cut(s) 154, 370, 456, 784
Hpy188III TCNNGA 4 cut(s) 59, 311, 416, 923
Hpy8I GTNNAC 3 cut(s) 858, 1058, 1301
HpyAV CCTTC 2 cut(s) 698, 1022
HpyCH4III ACNGT 2 cut(s) 883, 1144
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
HpyF3I CTNAG 7 cut(s) 107, 318, 755, 781, 844, 1045, 1328
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 1 cut(s) 409
Kzo9I GATC 4 cut(s) 307, 477, 925, 1252
LmnI GCTCC 3 cut(s) 680, 1350, 1357
Lsp1109I GCAGC 5 cut(s) 33, 630, 683, 835, 1159
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 5 cut(s) 758, 848, 966, 984, 1025
MalI GATC 4 cut(s) 309, 479, 927, 1254
MboI GATC 4 cut(s) 307, 477, 925, 1252
MboII GAAGA 6 cut(s) 61, 513, 909, 1088, 1108, 1213
MhlI GDGCHC 4 cut(s) 142, 194, 685, 1347
MluCI AATT 4 cut(s) 223, 358, 525, 732
MlyI GAGTC 2 cut(s) 272, 1073
MmeI TCCRAC 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 1173
MroXI GAANNNNTTC 2 cut(s) 789, 1103
MseI TTAA 1 cut(s) 1368
MslI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 2 cut(s) 1093, 1228
MspR9I CCNGG 3 cut(s) 340, 637, 999
MvaI CCWGG 3 cut(s) 340, 637, 999
MwoI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 173
NdeII GATC 4 cut(s) 307, 477, 925, 1252
NlaIV GGNNCC 2 cut(s) 193, 874
NmeAIII GCCGAG 1 cut(s) 1010
NmuCI GTSAC 3 cut(s) 758, 848, 1025
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 3 cut(s) 519, 609, 696
OliI CACNNNNGTG 1 cut(s) 428
PaeI GCATGC 2 cut(s) 609, 696
PagI TCATGA 1 cut(s) 922
PasI CCCWGGG 1 cut(s) 339
PciI ACATGT 1 cut(s) 515
PdmI GAANNNNTTC 2 cut(s) 789, 1103
PfeI GAWTC 1 cut(s) 98
PflMI CCANNNNNTGG 1 cut(s) 1022
PfoI TCCNGGA 2 cut(s) 635, 997
PkrI GCNGC 6 cut(s) 23, 645, 691, 698, 825, 1149
PleI GAGTC 2 cut(s) 272, 1073
PpsI GAGTC 2 cut(s) 272, 1073
PscI ACATGT 1 cut(s) 515
Psp124BI GAGCTC 3 cut(s) 142, 685, 1347
Psp6I CCWGG 3 cut(s) 338, 635, 997
PspFI CCCAGC 4 cut(s) 672, 835, 1318, 1322
PspGI CCWGG 3 cut(s) 338, 635, 997
PspN4I GGNNCC 2 cut(s) 193, 874
PspPI GGNCC 1 cut(s) 342
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 2 cut(s) 152, 317
PvuII CAGCTG 1 cut(s) 1322
RsaI GTAC 4 cut(s) 16, 170, 802, 975
RsaNI GTAC 4 cut(s) 15, 169, 801, 974
RseI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
SacI GAGCTC 3 cut(s) 142, 685, 1347
SaqAI TTAA 1 cut(s) 1368
SatI GCNGC 6 cut(s) 22, 644, 690, 697, 824, 1148
Sau3AI GATC 4 cut(s) 307, 477, 925, 1252
Sau96I GGNCC 1 cut(s) 342
ScaI AGTACT 1 cut(s) 975
SchI GAGTC 2 cut(s) 272, 1073
ScrFI CCNGG 3 cut(s) 340, 637, 999
SduI GDGCHC 4 cut(s) 142, 194, 685, 1347
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 1125
SmiMI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
SmlI CTYRAG 2 cut(s) 57, 1367
SmoI CTYRAG 2 cut(s) 57, 1367
SphI GCATGC 2 cut(s) 609, 696
Sse9I AATT 4 cut(s) 223, 358, 525, 732
SsiI CCGC 4 cut(s) 574, 689, 739, 1284
SspI AATATT 1 cut(s) 994
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 3 cut(s) 142, 685, 1347
StyD4I CCNGG 3 cut(s) 338, 635, 997
StyI CCWWGG 3 cut(s) 242, 619, 724
TaaI ACNGT 2 cut(s) 883, 1144
TaiI ACGT 1 cut(s) 857
TasI AATT 4 cut(s) 223, 358, 525, 732
TatI WGTACW 2 cut(s) 168, 973
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 1368
Tru9I TTAA 1 cut(s) 1368
TseFI GTSAC 3 cut(s) 758, 848, 1025
TseI GCWGC 5 cut(s) 21, 643, 696, 823, 1147
Tsp45I GTSAC 3 cut(s) 758, 848, 1025
TspDTI ATGAA 3 cut(s) 825, 911, 1226
Van91I CCANNNNNTGG 1 cut(s) 1022
Vha464I CTTAAG 1 cut(s) 1367
XapI RAATTY 1 cut(s) 223
XbaI TCTAGA 1 cut(s) 415
XceI RCATGY 3 cut(s) 519, 609, 696
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 731
XmnI GAANNNNTTC 2 cut(s) 789, 1103
XspI CTAG 1 cut(s) 416
ZrmI AGTACT 1 cut(s) 975
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.