pycom17g14000

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013494
Physical Location & Seq
Forward (+)
11581634 .. 11584448
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g14000.1

Sequence Viewer

Length: 1401 bp
ATGGCAGAAACCAGCAACACTAGCAGAAGCAGCAGCAGCTTCTGCTACTCCTTCAATGGCTCACCAATTTCCAACATAGCTCAAGACCACCTCTTCACAATCCTGCTGCTCCTTCCTGTGGATTCAATTCTTTGCTTTGCCATGACCTGCAAAAAGTTCAGAGCTTTGGCAGCATCTGACAGTCTGTGGGAGTCCATATGCAGGAGGGACTGGGGACCCAAATCAGTGGAGGCCCTCAAAGCTTCAAACTTTGGCCATCAGCAACTGCCATGGATGACGCTCTACAAGCAAGTATCTAGGGTTGACTCTGTGTATTGCCATAAATTTTCTGACCCGGATGGGGAATTGGGGTTCCCCAGTCCCAGGGCTTCTCATTCCCTCAATTTTGTGTCTGGTTGCTTGGTGTTGTTTGGTGGTGGATGTGAGGGAGGACGCCATCTAGACGACACATGGGTGGCATACATTGGTAATGATTTTCGGAGAATGTTGAAGTGGCAGAAGATAAATTCTGGCATTCCAAGTGGTAGATTTGGGCATTCTTCTGTTGTAATTGATGATTTTCTTGTTATGTTTGGAGGAATAAATGACAATGGGGGCCGTCACAATGATACATGGGTGGGGCAAGTAGAATGCCATGGGAATCTTGGTGTCACACTCTCATGGAAGCTGCTTGTTGTGGGATCTGATGCACCTTCCCAGCGAGGGGCTCATGCTGCATGTTGCATTGACGGCAGAAAGATGGTTATCCATGGAGGAATTGGGCTTTATGGTGTGAGATTGTGTGACACATGGGTGTTGGAACTCTCAGAAAATTTTCGGTTTGGAACTTGGTATGAAATCAGGACTCATCCATGTCCCCCAGCTCGTTCGGGACACACACTGACCTGCATCGGAGGAGCACGAACGGTTTTATTTGGTGGGAGAGGATTAGGTTACGATGTGCTTAATGATGTCTGGTTCTTGGACATTTGTGAAGGTAATCCGAAATGGGTACAGGTACACTATGAGCTACAAAATATTCCAGGAGGAGTCTCTCTTCCGCGAGTTGGTCACTCGGCCACGCTCATGTTAGGCGGCCATCTGCTGATCTGTGGGGGAGAGGATTCGTACAGACACCGTAAAAATGACTTCTGGGTTTTAGACATAAATGCAGTTCCATCCATTACAATGCAGCAGCCAACTCCACTGAACTCTATGCAGTTATTGGCAAAAATGTGGAAGAGGTTCAAGGCAAATGGTTATGAGCCCAAATGCCGGTCATTCCATGGAGCCTGCACAGATAACTCAGGGCGGTACTTGTTTGTGTTTGGCGGAATGGTGGATGGGTTGCTTCAGCCGGGTGGCCCTGCCGGGCTGAGGTTTGACGGCGAACTCCTGCTTGTGGAGCTTGTGCTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_3 Kelch_4 Kelch_5

Protein Analysis

467

Amino Acids

51.4

Weight (kDa)

7.86

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 31 - 69 4e-06 F-box-like
Beta-prop_FBX42 PF13415 110 - 271 2e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 117 - 208 9.6e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 164 - 252 6.2e-08 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 164 - 440 3.9e-13 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 218 - 383 1e-26 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 271 - 440 5.8e-08 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 276 - 383 4.1e-15 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 286 - 383 1e-08 tRNA wybutosine-synthesizing protein 4, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51550
fragaria_vesca FvH4_6g37110
malus_domestica MD09G1161500.v1.1 MD17G1147400.v1.1
prunus_persica Prupe.3G020600_v2.0.a1
pyrus_communis pycom09g08000 pycom09g08050 pycom17g14000
rosa_chinensis RchiOBHm_Chr2g0149671
rosa_laevigata RLG00000020436
rosa_multiflora Rmu_sc0014307.1_g000005
rosa_roxburghii Rroxscaffold_2G00097810
rosa_rugosa Rorug02G0414300
rosa_samantha Rh2AG474500 Rh2BG486200 Rh2CG460200 Rh2DG495500
rosa_wichuraiana Rw2G038690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 155, 893
AccII CGCG 1 cut(s) 1042
AciI CCGC 4 cut(s) 1040, 1074, 1291, 1311
AclWI GGATC 1 cut(s) 688
AcoI YGGCCR 3 cut(s) 253, 1056, 1075
AcsI RAATTY 3 cut(s) 323, 505, 811
AcuI CTGAAG 1 cut(s) 1316
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 4 cut(s) 993, 999, 1109, 1295
AgsI TTSAA 5 cut(s) 55, 126, 246, 490, 1228
AjnI CCWGG 2 cut(s) 362, 1021
AleI CACNNNNGTG 2 cut(s) 452, 791
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 8 cut(s) 39, 80, 164, 242, 667, 863, 1009, 1387
AluI AGCT 8 cut(s) 39, 80, 164, 242, 667, 863, 1009, 1387
Alw21I GWGCWC 1 cut(s) 901
Alw26I GTCTC 1 cut(s) 1036
AlwI GGATC 1 cut(s) 688
AlwNI CAGNNNCTG 3 cut(s) 42, 176, 265
AoxI GGCC 6 cut(s) 231, 253, 595, 1056, 1075, 1342
ApeKI GCWGC 9 cut(s) 30, 33, 36, 106, 170, 667, 713, 1171, 1174
ApoI RAATTY 3 cut(s) 323, 505, 811
Asp700I GAANNNNTTC 2 cut(s) 813, 1223
AspS9I GGNCC 4 cut(s) 215, 232, 595, 1343
AsuC2I CCSGG 3 cut(s) 335, 1338, 1351
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BalI TGGCCA 1 cut(s) 255
BanII GRGCYC 2 cut(s) 709, 1248
Bbv12I GWGCWC 1 cut(s) 901
BbvCI CCTCAGC 2 cut(s) 1355, 1394
BbvI GCAGC 9 cut(s) 42, 45, 48, 93, 182, 654, 700, 1183, 1186
BccI CCATC 7 cut(s) 264, 332, 444, 733, 1086, 1165, 1316
BceAI ACGGC 3 cut(s) 582, 745, 1381
BciT130I CCWGG 2 cut(s) 364, 1023
BcnI CCSGG 3 cut(s) 335, 1338, 1351
BcoDI GTCTC 1 cut(s) 1036
BfaI CTAG 3 cut(s) 21, 297, 440
BfuAI ACCTGC 2 cut(s) 155, 893
Bme1390I CCNGG 5 cut(s) 335, 364, 1023, 1338, 1351
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 4 cut(s) 215, 232, 595, 1343
BmiI GGNNCC 5 cut(s) 216, 217, 353, 596, 1270
BmrFI CCNGG 5 cut(s) 335, 364, 1023, 1338, 1351
BmrI ACTGGG 2 cut(s) 220, 351
BmsI GCATC 3 cut(s) 182, 676, 897
BmuI ACTGGG 2 cut(s) 220, 351
Bpu10I CCTNAGC 2 cut(s) 1355, 1394
BpuEI CTTGAG 1 cut(s) 66
BpuMI CCSGG 3 cut(s) 335, 1338, 1351
BsaBI GATNNNNATC 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 433
BsaJI CCNNGG 6 cut(s) 269, 362, 363, 634, 748, 1264
Bse118I RCCGGY 1 cut(s) 1254
Bse1I ACTGG 2 cut(s) 215, 357
Bse8I GATNNNNATC 1 cut(s) 743
BseBI CCWGG 2 cut(s) 364, 1023
BseDI CCNNGG 6 cut(s) 269, 362, 363, 634, 748, 1264
BseGI GGATG 6 cut(s) 279, 343, 425, 847, 1157, 1327
BseJI GATNNNNATC 1 cut(s) 743
BseMII CTCAG 4 cut(s) 819, 1299, 1346, 1385
BseNI ACTGG 2 cut(s) 215, 357
BseRI GAGGAG 2 cut(s) 909, 1041
BseXI GCAGC 9 cut(s) 42, 45, 48, 93, 182, 654, 700, 1183, 1186
BseYI CCCAGC 2 cut(s) 696, 859
BsgI GTGCAG 1 cut(s) 1258
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 6 cut(s) 233, 255, 597, 1058, 1077, 1344
BsiHKAI GWGCWC 1 cut(s) 901
BsiSI CCGG 4 cut(s) 335, 1255, 1337, 1350
BslFI GGGAC 5 cut(s) 221, 228, 345, 840, 885
BsmAI GTCTC 1 cut(s) 1036
BsmFI GGGAC 5 cut(s) 221, 228, 345, 840, 885
BsmI GAATGC 3 cut(s) 513, 535, 635
BsnI GGCC 6 cut(s) 233, 255, 597, 1058, 1077, 1344
Bsp1286I GDGCHC 3 cut(s) 709, 901, 1248
Bsp143I GATC 2 cut(s) 680, 1086
Bsp19I CCATGG 4 cut(s) 269, 634, 748, 1264
BspACI CCGC 4 cut(s) 1040, 1074, 1291, 1311
BspANI GGCC 6 cut(s) 233, 255, 597, 1058, 1077, 1344
BspCNI CTCAG 4 cut(s) 818, 1298, 1347, 1386
BspFNI CGCG 1 cut(s) 1042
BspLI GGNNCC 5 cut(s) 216, 217, 353, 596, 1270
BspMI ACCTGC 2 cut(s) 155, 893
BspPI GGATC 1 cut(s) 688
BsrFI RCCGGY 1 cut(s) 1254
BsrI ACTGG 2 cut(s) 215, 357
BssAI RCCGGY 1 cut(s) 1254
BssECI CCNNGG 6 cut(s) 269, 362, 363, 634, 748, 1264
BssMI GATC 2 cut(s) 680, 1086
BssNI GRCGYC 1 cut(s) 433
BssT1I CCWWGG 4 cut(s) 269, 634, 748, 1264
Bst2UI CCWGG 2 cut(s) 364, 1023
Bst4CI ACNGT 3 cut(s) 182, 907, 1118
Bst6I CTCTTC 3 cut(s) 98, 1041, 1214
BstACI GRCGYC 1 cut(s) 433
BstAPI GCANNNNNTGC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 1273
BstDEI CTNAG 4 cut(s) 805, 1285, 1355, 1394
BstDSI CCRYGG 4 cut(s) 269, 634, 748, 1264
BstF5I GGATG 6 cut(s) 279, 343, 425, 847, 1157, 1327
BstFNI CGCG 1 cut(s) 1042
BstKTI GATC 2 cut(s) 683, 1089
BstMAI GTCTC 1 cut(s) 1036
BstMBI GATC 2 cut(s) 680, 1086
BstNI CCWGG 2 cut(s) 364, 1023
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 5 cut(s) 333, 362, 1021, 1336, 1349
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 9 cut(s) 42, 45, 48, 93, 182, 654, 700, 1183, 1186
BstX2I RGATCY 1 cut(s) 680
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 6 cut(s) 233, 255, 597, 1058, 1077, 1344
BtgI CCRYGG 4 cut(s) 269, 634, 748, 1264
BtsCI GGATG 6 cut(s) 279, 343, 425, 847, 1157, 1327
BtsIMutI CAGTG 3 cut(s) 231, 878, 1184
BveI ACCTGC 2 cut(s) 155, 893
Cac8I GCNNGC 1 cut(s) 1273
CaiI CAGNNNCTG 3 cut(s) 42, 176, 265
Cfr10I RCCGGY 1 cut(s) 1254
Cfr13I GGNCC 4 cut(s) 215, 232, 595, 1343
CseI GACGC 2 cut(s) 286, 441
Csp6I GTAC 4 cut(s) 992, 998, 1108, 1294
CviQI GTAC 4 cut(s) 992, 998, 1108, 1294
DdeI CTNAG 4 cut(s) 805, 1285, 1355, 1394
DpnI GATC 2 cut(s) 682, 1088
DpnII GATC 2 cut(s) 680, 1086
EaeI YGGCCR 3 cut(s) 253, 1056, 1075
Eam1104I CTCTTC 3 cut(s) 98, 1041, 1214
EarI CTCTTC 3 cut(s) 98, 1041, 1214
EciI GGCGGA 1 cut(s) 1326
Eco130I CCWWGG 4 cut(s) 269, 634, 748, 1264
Eco24I GRGCYC 2 cut(s) 709, 1248
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 1316
EcoO109I RGGNCCY 2 cut(s) 215, 232
EcoRII CCWGG 2 cut(s) 362, 1021
EcoT14I CCWWGG 4 cut(s) 269, 634, 748, 1264
EcoT38I GRGCYC 2 cut(s) 709, 1248
ErhI CCWWGG 4 cut(s) 269, 634, 748, 1264
FaqI GGGAC 5 cut(s) 221, 228, 345, 840, 885
FauNDI CATATG 1 cut(s) 197
FokI GGATG 6 cut(s) 286, 350, 432, 834, 1144, 1334
FriOI GRGCYC 2 cut(s) 709, 1248
FspBI CTAG 3 cut(s) 21, 297, 440
GsaI CCCAGC 2 cut(s) 700, 863
HaeIII GGCC 6 cut(s) 233, 255, 597, 1058, 1077, 1344
HapII CCGG 4 cut(s) 335, 1255, 1337, 1350
HgaI GACGC 2 cut(s) 286, 441
Hin1I GRCGYC 1 cut(s) 433
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 7 cut(s) 122, 191, 305, 640, 844, 1029, 1103
HpaII CCGG 4 cut(s) 335, 1255, 1337, 1350
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 2 cut(s) 304, 1000
Hpy188I TCNGA 8 cut(s) 161, 178, 331, 480, 685, 808, 893, 984
Hpy188III TCNNGA 4 cut(s) 83, 440, 841, 870
Hpy8I GTNNAC 2 cut(s) 304, 1000
HpyAV CCTTC 4 cut(s) 61, 122, 702, 968
HpyCH4III ACNGT 3 cut(s) 182, 907, 1118
HpyF3I CTNAG 4 cut(s) 805, 1285, 1355, 1394
Hsp92I GRCGYC 1 cut(s) 433
KflI GGGWCCC 1 cut(s) 215
Kzo9I GATC 2 cut(s) 680, 1086
LmnI GCTCC 4 cut(s) 114, 896, 1268, 1384
Lsp1109I GCAGC 9 cut(s) 42, 45, 48, 93, 182, 654, 700, 1183, 1186
LweI GCATC 3 cut(s) 182, 676, 897
MaeI CTAG 3 cut(s) 21, 297, 440
MaeIII GTNAC 5 cut(s) 599, 649, 782, 932, 1049
MalI GATC 2 cut(s) 682, 1088
MboI GATC 2 cut(s) 680, 1086
MboII GAAGA 5 cut(s) 85, 511, 531, 1028, 1231
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 3 cut(s) 709, 901, 1248
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 9 cut(s) 66, 126, 323, 344, 382, 505, 549, 756, 811
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 4 cut(s) 200, 299, 838, 1038
MmeI TCCRAC 2 cut(s) 96, 777
Mox20I TGGCCA 1 cut(s) 255
MroXI GAANNNNTTC 2 cut(s) 813, 1223
MscI TGGCCA 1 cut(s) 255
MseI TTAA 1 cut(s) 945
MslI CAYNNNNRTG 5 cut(s) 452, 658, 791, 1064, 1166
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 4 cut(s) 335, 1255, 1337, 1350
MspR9I CCNGG 5 cut(s) 335, 364, 1023, 1338, 1351
Mva1269I GAATGC 3 cut(s) 513, 535, 635
MvaI CCWGG 2 cut(s) 364, 1023
MvnI CGCG 1 cut(s) 1042
NciI CCSGG 3 cut(s) 335, 1338, 1351
NcoI CCATGG 4 cut(s) 269, 634, 748, 1264
NdeI CATATG 1 cut(s) 197
NdeII GATC 2 cut(s) 680, 1086
NlaIV GGNNCC 5 cut(s) 216, 217, 353, 596, 1270
NmeAIII GCCGAG 1 cut(s) 1034
NmuCI GTSAC 4 cut(s) 599, 649, 782, 1049
NspI RCATGY 1 cut(s) 720
OliI CACNNNNGTG 2 cut(s) 452, 791
PasI CCCWGGG 1 cut(s) 363
PctI GAATGC 3 cut(s) 513, 535, 635
PdmI GAANNNNTTC 2 cut(s) 813, 1223
PfeI GAWTC 3 cut(s) 122, 640, 1103
PfoI TCCNGGA 1 cut(s) 1021
PleI GAGTC 4 cut(s) 199, 299, 838, 1037
PpsI GAGTC 4 cut(s) 199, 299, 838, 1037
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 362, 1021
PspFI CCCAGC 2 cut(s) 696, 859
PspGI CCWGG 2 cut(s) 362, 1021
PspN4I GGNNCC 5 cut(s) 216, 217, 353, 596, 1270
PspPI GGNCC 4 cut(s) 215, 232, 595, 1343
PspPPI RGGWCCY 1 cut(s) 215
PstNI CAGNNNCTG 3 cut(s) 42, 176, 265
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 4 cut(s) 993, 999, 1109, 1295
RsaNI GTAC 4 cut(s) 992, 998, 1108, 1294
RseI CAYNNNNRTG 5 cut(s) 452, 658, 791, 1064, 1166
SaqAI TTAA 1 cut(s) 945
Sau3AI GATC 2 cut(s) 680, 1086
Sau96I GGNCC 4 cut(s) 215, 232, 595, 1343
SchI GAGTC 4 cut(s) 200, 299, 838, 1038
ScrFI CCNGG 5 cut(s) 335, 364, 1023, 1338, 1351
SduI GDGCHC 3 cut(s) 709, 901, 1248
SfaNI GCATC 3 cut(s) 182, 676, 897
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 5 cut(s) 452, 658, 791, 1064, 1166
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 9 cut(s) 66, 126, 323, 344, 382, 505, 549, 756, 811
SsiI CCGC 4 cut(s) 1040, 1074, 1291, 1311
SspI AATATT 1 cut(s) 1018
SspMI CTAG 3 cut(s) 21, 297, 440
StyD4I CCNGG 5 cut(s) 333, 362, 1021, 1336, 1349
StyI CCWWGG 4 cut(s) 269, 634, 748, 1264
TaaI ACNGT 3 cut(s) 182, 907, 1118
TasI AATT 9 cut(s) 66, 126, 323, 344, 382, 505, 549, 756, 811
TauI GCSGC 1 cut(s) 1077
TfiI GAWTC 3 cut(s) 122, 640, 1103
Tru1I TTAA 1 cut(s) 945
Tru9I TTAA 1 cut(s) 945
TscAI CASTG 3 cut(s) 231, 885, 1191
TseFI GTSAC 4 cut(s) 599, 649, 782, 1049
TseI GCWGC 9 cut(s) 30, 33, 36, 106, 170, 667, 713, 1171, 1174
Tsp45I GTSAC 4 cut(s) 599, 649, 782, 1049
TspDTI ATGAA 1 cut(s) 849
TspRI CASTG 3 cut(s) 231, 885, 1191
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 3 cut(s) 323, 505, 811
XbaI TCTAGA 1 cut(s) 439
XceI RCATGY 1 cut(s) 720
XcmI CCANNNNNNNNNTGG 2 cut(s) 641, 755
XmnI GAANNNNTTC 2 cut(s) 813, 1223
XspI CTAG 3 cut(s) 21, 297, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.