Rorug02G0414300

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001653
Physical Location & Seq
Forward (+)
53027726 .. 53033545
5820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0414300.1

Sequence Viewer

Length: 1623 bp
ATGGCTAACTCTGCTTCTTCCATTTCCACCACCACTCAGCGGTTCCGCTGCTTCGACCCCACCAACTCTTACGCTGCGACGCCCGATCACCCTCGCCGCTTCAGCACCACCTCCAATTCAACTCCCAGGTACTACAACAACAACAACTCCAAGGGCACTATGGGAATTTCCGCCAACTCCGAGCCCAAGATCATCACCGGCGACACTGGCTACGTTCTCGAAGACGTCCCTCACTTGTCCGATTACATCCCCCATCTCCCTACATATCCCAATCCATTACAGGACAATCCTTCCTATTCAGTTGTTAAGCAGTATTTCGTTAATGTCGATGATAGTGTTGCCCAGACGATAGTAGTCCAGAAAGATAGTCCAAGAGGCACACATTTTCGTCGTGCTGGACCGCGTCAGAAGGTCTATTTTGAACCAGATGATGTTCGTGCTTGTATTGTGACATGTGGTGGTTTATGTCCAGGCTTAAATACAGTAATTAGAGAAATAGTATGTGGGTTGTACCACATGTATGGTGTCCACCGAATTCTTGGAATAGAGGGAGGATACAGAGGATTTTATGCCAGAAATACAATTCCCTTAACACCAATGGTTGTGAATGACATCCATAAACGTGGTGGCACCATCCTTGGGACATCACGAGGTGGCCATGATACCTCAAAGATTGTGGACAGCATTCAGGACAGAGGAATTAATCAGGTTTACATACTCGGGGGAGATGGTACACAGAAAGGAGCTTCCGTAATATTTGAGGAATGCAGGAGGCGTGGCCTGAAAGTTGCAGTGGCAGGAATTCCAAAAACCATTGATAATGACATTCCGGTTATAGACAAGTCCTTTGGTTTTGACACTGCTGTTGAGGAGGCTCAACGAGCCATTAATGCAGCTCATGTTGAAGCAGAAAGCATTGAGAATGGTATTGGCGTTGTCAAGTTAATGGGTCGCTACAGTGGATTTATAGCTATGTATGCTACTCTTGCCAGCCGAGATGTGGATTGTTGCCTGATTCCAGAGTCGCCTTTCTATCTGGAAGGAGCAGGTGGACTGTTTGAATACATTGAAAAAAGACTTAAAGAGAATGGCCACATGGTTATAGTAATAGCTGAAGGCGCAGGACAAGAGCTTCTAGCTGAAAGCATGCTTGCCATGAATCATCAGGACGCTTCTGGAAATAAGCTTCTTCAAGATTCTGGTCTGTGGTTATCACAGAAGATTAAGGACCACTTCTCAAAACTGAACAAGATGTCTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCTATTCCAAGCAATGCATCTGATAATGTATACTGCACCCTCCTTGCTCATAGTGTGGTTCATGGTGCAATGGCAGGATACACCGGCTTCACCGTCGGCTCTGTTAATGGAAGGCATGCTTATATTCCTTTCCATCGGGTGACGGAGAGACAGAACAAGGTTGTGATTACTGACAGAATGTGGGCACGGCTATTATCATCAACTAATCAGCCAAGTTTCTTGGATCCGAAGGAAGTAGCTAAAAATAAAGGGGAAGAAACAGAAATCCACCTGGCCAATGGGGAAAACCATTTGGAGGAAACTGCTTCGGCTACGCCAAAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

540

Amino Acids

59.37

Weight (kDa)

6.66

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 147 - 453 1e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1037
AatII GACGTC 1 cut(s) 228
Acc36I ACCTGC 1 cut(s) 1037
AccB1I GGYRCC 1 cut(s) 629
AccI GTMKAC 1 cut(s) 1326
AccII CGCG 1 cut(s) 403
AciI CCGC 5 cut(s) 40, 46, 97, 171, 401
AclWI GGATC 4 cut(s) 1271, 1285, 1514, 1527
AcoI YGGCCR 3 cut(s) 655, 1090, 1569
AcsI RAATTY 3 cut(s) 165, 534, 801
AcuI CTGAAG 2 cut(s) 85, 1134
AcyI GRCGYC 2 cut(s) 80, 225
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 3 cut(s) 131, 512, 733
AfiI CCNNNNNNNGG 4 cut(s) 39, 639, 1000, 1591
AflIII ACRYGT 2 cut(s) 452, 516
AgsI TTSAA 6 cut(s) 120, 422, 905, 1061, 1070, 1193
AjnI CCWGG 3 cut(s) 125, 469, 1566
AluBI AGCT 8 cut(s) 746, 896, 971, 1112, 1132, 1139, 1186, 1535
AluI AGCT 8 cut(s) 746, 896, 971, 1112, 1132, 1139, 1186, 1535
Alw26I GTCTC 1 cut(s) 1438
AlwI GGATC 4 cut(s) 1271, 1285, 1514, 1527
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 4 cut(s) 655, 778, 1090, 1569
ApeKI GCWGC 3 cut(s) 48, 74, 893
ApoI RAATTY 3 cut(s) 165, 534, 801
ArsI GACNNNNNNTTYG 2 cut(s) 830, 862
AseI ATTAAT 2 cut(s) 702, 888
AspLEI GCGC 1 cut(s) 1121
AspS9I GGNCC 2 cut(s) 398, 1228
AsuHPI GGTGA 4 cut(s) 80, 187, 1378, 1447
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 398, 1228
BaeGI GKGCMC 2 cut(s) 158, 1483
BalI TGGCCA 3 cut(s) 657, 1092, 1571
BamHI GGATCC 1 cut(s) 1519
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 186
BauI CACGAG 1 cut(s) 648
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 35, 61, 905
BccI CCATC 4 cut(s) 261, 641, 722, 1437
BceAI ACGGC 1 cut(s) 1499
BciT130I CCWGG 3 cut(s) 127, 471, 1568
BciVI GTATCC 2 cut(s) 548, 1367
BcoDI GTCTC 1 cut(s) 1438
BfaI CTAG 1 cut(s) 1136
BfmI CTRYAG 1 cut(s) 955
BfuAI ACCTGC 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 548, 1367
BisI GCNGC 4 cut(s) 49, 75, 97, 894
BlsI GCNGC 4 cut(s) 50, 76, 98, 895
Bme1390I CCNGG 3 cut(s) 127, 471, 1568
Bme18I GGWCC 2 cut(s) 398, 1228
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 2 cut(s) 398, 1228
BmiI GGNNCC 3 cut(s) 44, 631, 1521
BmrFI CCNGG 3 cut(s) 127, 471, 1568
BmsI GCATC 1 cut(s) 1322
BpiI GAAGAC 1 cut(s) 228
BsaHI GRCGYC 2 cut(s) 80, 225
BsaJI CCNNGG 3 cut(s) 125, 150, 637
BsaWI WCCGGW 1 cut(s) 829
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 735, 765
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 639, 1000, 1591
Bse118I RCCGGY 2 cut(s) 197, 1379
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 2 cut(s) 1315, 1371
BseBI CCWGG 3 cut(s) 127, 471, 1568
BseDI CCNNGG 3 cut(s) 125, 150, 637
BseGI GGATG 3 cut(s) 246, 612, 633
BseLI CCNNNNNNNGG 4 cut(s) 39, 639, 1000, 1591
BseMI GCAATG 2 cut(s) 1315, 1371
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 884
BseSI GKGCMC 2 cut(s) 158, 1483
BseXI GCAGC 3 cut(s) 35, 61, 905
BsgI GTGCAG 1 cut(s) 1315
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 4 cut(s) 657, 780, 1092, 1571
BshNI GGYRCC 1 cut(s) 629
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 3 cut(s) 198, 830, 1380
BslFI GGGAC 2 cut(s) 212, 655
BslI CCNNNNNNNGG 4 cut(s) 39, 639, 1000, 1591
BsmAI GTCTC 1 cut(s) 1438
BsmFI GGGAC 2 cut(s) 212, 655
BsmI GAATGC 2 cut(s) 684, 770
BsnI GGCC 4 cut(s) 657, 780, 1092, 1571
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 3 cut(s) 158, 186, 1483
Bsp143I GATC 5 cut(s) 85, 189, 1276, 1290, 1519
BspACI CCGC 5 cut(s) 40, 46, 97, 171, 401
BspANI GGCC 4 cut(s) 657, 780, 1092, 1571
BspCNI CTCAG 1 cut(s) 49
BspFNI CGCG 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 44, 631, 1521
BspMI ACCTGC 1 cut(s) 1037
BspPI GGATC 4 cut(s) 1271, 1285, 1514, 1527
BspT107I GGYRCC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 1315, 1371
BsrFI RCCGGY 2 cut(s) 197, 1379
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 2 cut(s) 197, 1379
BssECI CCNNGG 3 cut(s) 125, 150, 637
BssMI GATC 5 cut(s) 85, 189, 1276, 1290, 1519
BssNAI GTATAC 1 cut(s) 1327
BssNI GRCGYC 2 cut(s) 80, 225
BssSI CACGAG 1 cut(s) 648
BssT1I CCWWGG 2 cut(s) 150, 637
Bst1107I GTATAC 1 cut(s) 1327
Bst2BI CACGAG 1 cut(s) 648
Bst2UI CCWGG 3 cut(s) 127, 471, 1568
Bst4CI ACNGT 4 cut(s) 484, 959, 1056, 1390
BstACI GRCGYC 2 cut(s) 80, 225
BstC8I GCNNGC 4 cut(s) 991, 1148, 1152, 1413
BstDEI CTNAG 1 cut(s) 36
BstF5I GGATG 3 cut(s) 246, 612, 633
BstFNI CGCG 1 cut(s) 403
BstHHI GCGC 1 cut(s) 1121
BstKTI GATC 5 cut(s) 88, 192, 1279, 1293, 1522
BstMAI GTCTC 1 cut(s) 1438
BstMBI GATC 5 cut(s) 85, 189, 1276, 1290, 1519
BstMWI GCNNNNNNNGC 8 cut(s) 11, 102, 207, 881, 890, 977, 986, 1118
BstNI CCWGG 3 cut(s) 127, 471, 1568
BstNSI RCATGY 4 cut(s) 456, 520, 1150, 1415
BstSCI CCNGG 3 cut(s) 125, 469, 1566
BstSFI CTRYAG 1 cut(s) 955
BstSLI GKGCMC 2 cut(s) 158, 1483
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 35, 61, 905
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 1276, 1519
BstXI CCANNNNNNTGG 2 cut(s) 521, 623
BstYI RGATCY 2 cut(s) 1276, 1519
BstZ17I GTATAC 1 cut(s) 1327
BsuI GTATCC 2 cut(s) 548, 1367
BsuRI GGCC 4 cut(s) 657, 780, 1092, 1571
BtsCI GGATG 3 cut(s) 246, 612, 633
BtsI GCAGTG 2 cut(s) 798, 858
BtsIMutI CAGTG 4 cut(s) 204, 798, 858, 964
BveI ACCTGC 1 cut(s) 1037
Cac8I GCNNGC 4 cut(s) 991, 1148, 1152, 1413
CfoI GCGC 1 cut(s) 1121
Cfr10I RCCGGY 2 cut(s) 197, 1379
Cfr13I GGNCC 2 cut(s) 398, 1228
CseI GACGC 3 cut(s) 88, 392, 1178
Csp6I GTAC 3 cut(s) 130, 511, 732
CspCI CAANNNNNGTGG 2 cut(s) 657, 692
CviQI GTAC 3 cut(s) 130, 511, 732
DdeI CTNAG 1 cut(s) 36
DpnI GATC 5 cut(s) 87, 191, 1278, 1292, 1521
DpnII GATC 5 cut(s) 85, 189, 1276, 1290, 1519
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 3 cut(s) 655, 1090, 1569
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 150, 637
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 398, 1228
Eco57I CTGAAG 2 cut(s) 85, 1134
Eco88I CYCGRG 1 cut(s) 719
EcoRI GAATTC 2 cut(s) 534, 801
EcoRII CCWGG 3 cut(s) 125, 469, 1566
EcoT14I CCWWGG 2 cut(s) 150, 637
EcoT22I ATGCAT 1 cut(s) 1315
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 2 cut(s) 150, 637
FalI AAGNNNNNCTT 4 cut(s) 1217, 1249, 1399, 1431
FaqI GGGAC 2 cut(s) 212, 655
FblI GTMKAC 1 cut(s) 1326
Fnu4HI GCNGC 4 cut(s) 49, 75, 97, 894
FokI GGATG 3 cut(s) 233, 599, 620
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 49, 75, 97, 894
FspBI CTAG 1 cut(s) 1136
GlaI GCGC 1 cut(s) 1120
GluI GCNGC 4 cut(s) 49, 75, 97, 894
HaeIII GGCC 4 cut(s) 657, 780, 1092, 1571
HapII CCGG 3 cut(s) 198, 830, 1380
HgaI GACGC 3 cut(s) 88, 392, 1178
HhaI GCGC 1 cut(s) 1121
Hin1I GRCGYC 2 cut(s) 80, 225
Hin6I GCGC 1 cut(s) 1119
HinP1I GCGC 1 cut(s) 1119
HindIII AAGCTT 1 cut(s) 1184
HinfI GANTC 4 cut(s) 1015, 1022, 1159, 1196
HpaII CCGG 3 cut(s) 198, 830, 1380
HphI GGTGA 4 cut(s) 80, 187, 1378, 1447
Hpy166II GTNNAC 6 cut(s) 529, 679, 712, 734, 1052, 1327
Hpy188I TCNGA 5 cut(s) 181, 241, 408, 1318, 1524
Hpy188III TCNNGA 9 cut(s) 218, 358, 648, 689, 1019, 1037, 1166, 1176, 1193
Hpy8I GTNNAC 6 cut(s) 529, 679, 712, 734, 1052, 1327
Hpy99I CGWCG 3 cut(s) 82, 393, 1394
HpyAV CCTTC 6 cut(s) 300, 403, 1034, 1109, 1401, 1519
HpyCH4III ACNGT 4 cut(s) 484, 959, 1056, 1390
HpyCH4IV ACGT 3 cut(s) 213, 225, 622
HpyCH4V TGCA 6 cut(s) 768, 791, 893, 1313, 1332, 1364
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 102, 207, 881, 890, 977, 986, 1118
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 3 cut(s) 213, 225, 622
Hsp92I GRCGYC 2 cut(s) 80, 225
HspAI GCGC 1 cut(s) 1119
Kzo9I GATC 5 cut(s) 85, 189, 1276, 1290, 1519
LmnI GCTCC 2 cut(s) 743, 1043
Lsp1109I GCAGC 3 cut(s) 35, 61, 905
LweI GCATC 1 cut(s) 1322
MaeI CTAG 1 cut(s) 1136
MaeII ACGT 3 cut(s) 213, 225, 622
MaeIII GTNAC 2 cut(s) 448, 1435
MalI GATC 5 cut(s) 87, 191, 1278, 1292, 1521
MboI GATC 5 cut(s) 85, 189, 1276, 1290, 1519
MboII GAAGA 5 cut(s) 9, 233, 1181, 1231, 1562
MflI RGATCY 2 cut(s) 1276, 1519
MhlI GDGCHC 3 cut(s) 158, 186, 1483
MlsI TGGCCA 3 cut(s) 657, 1092, 1571
MluCI AATT 7 cut(s) 115, 165, 486, 534, 582, 699, 801
MluNI TGGCCA 3 cut(s) 657, 1092, 1571
MlyI GAGTC 1 cut(s) 1031
Mox20I TGGCCA 3 cut(s) 657, 1092, 1571
Mph1103I ATGCAT 1 cut(s) 1315
MscI TGGCCA 3 cut(s) 657, 1092, 1571
MslI CAYNNNNRTG 2 cut(s) 519, 621
Msp20I TGGCCA 3 cut(s) 657, 1092, 1571
MspA1I CMGCKG 2 cut(s) 40, 48
MspI CCGG 3 cut(s) 198, 830, 1380
MspR9I CCNGG 3 cut(s) 127, 471, 1568
Mva1269I GAATGC 2 cut(s) 684, 770
MvaI CCWGG 3 cut(s) 127, 471, 1568
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 8 cut(s) 11, 102, 207, 881, 890, 977, 986, 1118
NdeII GATC 5 cut(s) 85, 189, 1276, 1290, 1519
NlaIV GGNNCC 3 cut(s) 44, 631, 1521
NmeAIII GCCGAG 1 cut(s) 1019
NmuCI GTSAC 2 cut(s) 448, 1435
NsiI ATGCAT 1 cut(s) 1315
NspI RCATGY 4 cut(s) 456, 520, 1150, 1415
PaeI GCATGC 2 cut(s) 1150, 1415
PaqCI CACCTGC 1 cut(s) 1037
PciI ACATGT 2 cut(s) 452, 516
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 2 cut(s) 684, 770
PfeI GAWTC 3 cut(s) 1015, 1159, 1196
PflFI GACNNNGTC 1 cut(s) 402
PkrI GCNGC 4 cut(s) 50, 76, 98, 895
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PscI ACATGT 2 cut(s) 452, 516
PshBI ATTAAT 2 cut(s) 702, 888
Psp6I CCWGG 3 cut(s) 125, 469, 1566
PspGI CCWGG 3 cut(s) 125, 469, 1566
PspN4I GGNNCC 3 cut(s) 44, 631, 1521
PspPI GGNCC 2 cut(s) 398, 1228
PsuI RGATCY 2 cut(s) 1276, 1519
PsyI GACNNNGTC 1 cut(s) 402
RsaI GTAC 3 cut(s) 131, 512, 733
RsaNI GTAC 3 cut(s) 130, 511, 732
RseI CAYNNNNRTG 2 cut(s) 519, 621
SatI GCNGC 4 cut(s) 49, 75, 97, 894
Sau3AI GATC 5 cut(s) 85, 189, 1276, 1290, 1519
Sau96I GGNCC 2 cut(s) 398, 1228
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 3 cut(s) 127, 471, 1568
SduI GDGCHC 3 cut(s) 158, 186, 1483
SfaNI GCATC 1 cut(s) 1322
SfcI CTRYAG 1 cut(s) 955
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 2 cut(s) 398, 1228
SmiMI CAYNNNNRTG 2 cut(s) 519, 621
SphI GCATGC 2 cut(s) 1150, 1415
Sse9I AATT 7 cut(s) 115, 165, 486, 534, 582, 699, 801
SsiI CCGC 5 cut(s) 40, 46, 97, 171, 401
SspI AATATT 1 cut(s) 756
SspMI CTAG 1 cut(s) 1136
StyD4I CCNGG 3 cut(s) 125, 469, 1566
StyI CCWWGG 2 cut(s) 150, 637
TaaI ACNGT 4 cut(s) 484, 959, 1056, 1390
TaiI ACGT 3 cut(s) 216, 228, 625
TaqI TCGA 3 cut(s) 54, 219, 327
TasI AATT 7 cut(s) 115, 165, 486, 534, 582, 699, 801
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 3 cut(s) 1015, 1159, 1196
TscAI CASTG 4 cut(s) 211, 798, 865, 964
TseFI GTSAC 2 cut(s) 448, 1435
TseI GCWGC 3 cut(s) 48, 74, 893
Tsp45I GTSAC 2 cut(s) 448, 1435
TspDTI ATGAA 2 cut(s) 1172, 1346
TspGWI ACGGA 3 cut(s) 739, 1283, 1454
TspRI CASTG 4 cut(s) 211, 798, 865, 964
Tth111I GACNNNGTC 1 cut(s) 402
VpaK11BI GGWCC 2 cut(s) 398, 1228
VspI ATTAAT 2 cut(s) 702, 888
XapI RAATTY 3 cut(s) 165, 534, 801
XceI RCATGY 4 cut(s) 456, 520, 1150, 1415
XcmI CCANNNNNNNNNTGG 5 cut(s) 157, 536, 623, 997, 1571
XmiI GTMKAC 1 cut(s) 1326
XspI CTAG 1 cut(s) 1136
ZraI GACGTC 1 cut(s) 226
Zsp2I ATGCAT 1 cut(s) 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.