RH2CG460000

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0001653
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG460000.1

Sequence Viewer

Length: 1458 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGTCTCTACGTGTCAGGCAGGCCTGAGCAGCCAAAAGAAAGAGCACCTACAATGGGCTCAACTGCCATGCCCTATACTGGTGGTGATATCAAGAAGTCGGGAGAGCTGGGTAAAATGTTTGATATCCCGTCAATGGATGGCTCCAAAACTAGGAAATCTGGACAATTGACTGGTGCTCCTTCAAGGACCGGATCATTTGGAGGTGCTGCTTCACATTCAGGACCAATTCCTAACGCTGCAGGTCGTGCCGGCTATACTACATCAGGTCCCATATCTGGAGGCATGCCTGGTTCAGCTTCCGTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCCATGAAGAAGTCATCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCTGGGCCTCTTCCCCCCGTACTCCCCACCACAGGTCTCATTACTTCTGGACCCATTTCTTCTGGCCCACTAAATGCTTCTGGGGCCCCTCGAAAGGTATCCGGTCCTTTGGAGTCAATGGGATCAATGAAAGTACAAGGTTCCTCTCTTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGATGAGTTTTCATTTAGGAAAAACTTCCCAAAGCCAATATTATGGTCTTTGATTCTGCTTTTCATGATGGGGTTCATAGCTGGTGGCTTTATTCTCGGTGCAGTCCATAATCCAATTCTCCTCGTCGTGGTTGTGATCCTTTTCAGTGCAGTTGCTGCACTATTCACATGGAATACTTATTGGGGAAGAAGAGCTATTATAGGTTACATTGCTAGTTATTCGGATTCTGAGCTCAGAACTGCCAAGAATGGGCAATTTGTGAAGGTGTCAGGGGTGGTTACTTGTGGCAATGTGCCTCTCGAGTCATCCTTTCAAAAGGTTCCTAGATGTGTGTATACATCCACAAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCGTCGTTTTTCTTGGGGGCTCAGATCCATGGAAAGACGTGTGGTAGACTTCTACATCTCTGATTTCCAATCTGGGTTGAGAGCATTGGTTAAGACAGGCTGTGGAGCAAGGGTGACACCTTATGTTGATGATTCAATCGTCATTGATGTGAATCCCGTTAATGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGAGGGAGAGGAACCTTTCAAGTGATGATCGTGTGATGCAGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTCAGTGTAATGGGGGTCGTCCAGAGAAATGAAAATGTGTTGATGATTGTTCCTCCACCTGAGCCGATCACAACGGGATGCCAATGGGCCAAATGTATCTTCCCAGCTAGCCTTGAGGGCATCGTTTTGAGATGCGATGATGCATCAAAGAATGATGTCATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

485

Amino Acids

51.94

Weight (kDa)

9.54

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 2 cut(s) 959, 1065
AciI CCGC 1 cut(s) 430
AclWI GGATC 4 cut(s) 235, 568, 754, 1038
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 1 cut(s) 349
AfaI GTAC 3 cut(s) 459, 573, 1268
AflIII ACRYGT 2 cut(s) 45, 1057
AgsI TTSAA 6 cut(s) 219, 938, 999, 1155, 1233, 1258
AjiI CACGTC 1 cut(s) 1058
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 1370, 1402
AluBI AGCT 8 cut(s) 28, 142, 332, 704, 818, 856, 1004, 1394
AluI AGCT 8 cut(s) 28, 142, 332, 704, 818, 856, 1004, 1394
Alw21I GWGCWC 3 cut(s) 82, 214, 858
Alw26I GTCTC 3 cut(s) 44, 479, 1197
AlwI GGATC 4 cut(s) 235, 568, 754, 1038
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 923
AoxI GGCC 7 cut(s) 56, 427, 443, 502, 522, 600, 1374
ApaI GGGCCC 1 cut(s) 526
ApeKI GCWGC 5 cut(s) 64, 242, 272, 779, 1004
ApoI RAATTY 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 647
AsuHPI GGTGA 2 cut(s) 131, 1144
AsuNHI GCTAGC 1 cut(s) 1394
AvaI CYCGRG 1 cut(s) 923
AvaII GGWCC 7 cut(s) 222, 257, 302, 356, 398, 488, 542
BaeGI GKGCMC 1 cut(s) 526
BanII GRGCYC 4 cut(s) 95, 526, 858, 1041
Bbv12I GWGCWC 3 cut(s) 82, 214, 858
BbvI GCAGC 5 cut(s) 76, 229, 259, 766, 991
BccI CCATC 4 cut(s) 25, 167, 685, 984
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 44, 479, 1197
BfaI CTAG 4 cut(s) 186, 837, 948, 1395
BfmI CTRYAG 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 266
BfuI GTATCC 1 cut(s) 547
BglI GCCNNNNNGGC 1 cut(s) 1404
BisI GCNGC 6 cut(s) 65, 243, 273, 430, 780, 1005
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 6 cut(s) 66, 244, 274, 431, 781, 1006
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 7 cut(s) 222, 257, 302, 356, 398, 488, 542
BmeT110I CYCGRG 1 cut(s) 923
BmgBI CACGTC 1 cut(s) 1058
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 6 cut(s) 1239, 1355, 1409, 1416, 1417, 1439
BmtI GCTAGC 1 cut(s) 1398
BpmI CTGGAG 2 cut(s) 333, 1179
Bpu10I CCTNAGC 2 cut(s) 60, 1347
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 1421
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 2 cut(s) 16, 1170
BsaI GGTCTC 2 cut(s) 44, 479
BsaJI CCNNGG 1 cut(s) 1047
BsaWI WCCGGW 2 cut(s) 224, 539
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 1116, 1146
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 4 cut(s) 118, 211, 430, 1451
Bse3DI GCAATG 3 cut(s) 40, 831, 919
Bse8I GATNNNNATC 2 cut(s) 16, 1170
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 1047
BseGI GGATG 5 cut(s) 178, 929, 962, 995, 1370
BseJI GATNNNNATC 2 cut(s) 16, 1170
BseMI GCAATG 3 cut(s) 40, 831, 919
BseMII CTCAG 6 cut(s) 43, 51, 843, 871, 1054, 1338
BseNI ACTGG 4 cut(s) 118, 211, 430, 1451
BseRI GAGGAG 1 cut(s) 734
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 5 cut(s) 76, 229, 259, 766, 991
BseYI CCCAGC 2 cut(s) 142, 1390
BsgI GTGCAG 3 cut(s) 744, 765, 792
BshFI GGCC 7 cut(s) 58, 429, 445, 504, 524, 602, 1376
BsiHKAI GWGCWC 3 cut(s) 82, 214, 858
BsiHKCI CYCGRG 1 cut(s) 923
BsiSI CCGG 3 cut(s) 225, 285, 540
BslFI GGGAC 1 cut(s) 288
BsmAI GTCTC 3 cut(s) 44, 479, 1197
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 7 cut(s) 58, 429, 445, 504, 524, 602, 1376
Bso31I GGTCTC 2 cut(s) 44, 479
BsoBI CYCGRG 1 cut(s) 923
Bsp120I GGGCCC 1 cut(s) 522
Bsp1286I GDGCHC 6 cut(s) 82, 95, 214, 526, 858, 1041
Bsp143I GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
Bsp1720I GCTNAGC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 1 cut(s) 430
BspANI GGCC 7 cut(s) 58, 429, 445, 504, 524, 602, 1376
BspCNI CTCAG 6 cut(s) 42, 52, 844, 870, 1053, 1339
BspHI TCATGA 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 266
BspOI GCTAGC 1 cut(s) 1398
BspPI GGATC 4 cut(s) 235, 568, 754, 1038
BspQI GCTCTTC 1 cut(s) 808
BspTNI GGTCTC 2 cut(s) 44, 479
BsrDI GCAATG 3 cut(s) 40, 831, 919
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 4 cut(s) 118, 211, 430, 1451
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 1047
BssMI GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
BssNAI GTATAC 1 cut(s) 960
BssT1I CCWWGG 1 cut(s) 1047
Bst1107I GTATAC 1 cut(s) 960
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 1288
Bst6I CTCTTC 3 cut(s) 453, 808, 1179
BstAPI GCANNNNNTGC 2 cut(s) 281, 779
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 56, 286, 320, 1396
BstDEI CTNAG 7 cut(s) 29, 60, 614, 852, 857, 1040, 1347
BstDSI CCRYGG 1 cut(s) 1047
BstF5I GGATG 5 cut(s) 178, 929, 962, 995, 1370
BstKTI GATC 6 cut(s) 230, 563, 762, 1046, 1243, 1356
BstMAI GTCTC 3 cut(s) 44, 479, 1197
BstMBI GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
BstMWI GCNNNNNNNGC 5 cut(s) 64, 281, 521, 779, 1404
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 526
BstV1I GCAGC 5 cut(s) 76, 229, 259, 766, 991
BstX2I RGATCY 1 cut(s) 1043
BstXI CCANNNNNNTGG 2 cut(s) 440, 666
BstYI RGATCY 1 cut(s) 1043
BstZ17I GTATAC 1 cut(s) 960
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 7 cut(s) 58, 429, 445, 504, 524, 602, 1376
BtgI CCRYGG 1 cut(s) 1047
BtgZI GCGATG 1 cut(s) 1437
BtrI CACGTC 1 cut(s) 1058
BtsCI GGATG 5 cut(s) 178, 929, 962, 995, 1370
BtsIMutI CAGTG 3 cut(s) 359, 775, 1297
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 4 cut(s) 56, 286, 320, 1396
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 284
Csp6I GTAC 3 cut(s) 458, 572, 1267
CviAII CATG 7 cut(s) 103, 319, 371, 382, 688, 792, 1048
CviQI GTAC 3 cut(s) 458, 572, 1267
DdeI CTNAG 7 cut(s) 29, 60, 614, 852, 857, 1040, 1347
DpnI GATC 6 cut(s) 229, 562, 761, 1045, 1242, 1355
DpnII GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
EaeI YGGCCR 1 cut(s) 427
Eam1104I CTCTTC 3 cut(s) 453, 808, 1179
EarI CTCTTC 3 cut(s) 453, 808, 1179
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 1047
Eco147I AGGCCT 1 cut(s) 58
Eco24I GRGCYC 4 cut(s) 95, 526, 858, 1041
Eco31I GGTCTC 2 cut(s) 44, 479
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 7 cut(s) 222, 257, 302, 356, 398, 488, 542
Eco53kI GAGCTC 1 cut(s) 856
Eco88I CYCGRG 1 cut(s) 923
EcoICRI GAGCTC 1 cut(s) 856
EcoO109I RGGNCCY 3 cut(s) 302, 522, 523
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 2 cut(s) 124, 160
EcoT14I CCWWGG 1 cut(s) 1047
EcoT22I ATGCAT 1 cut(s) 1432
EcoT38I GRGCYC 4 cut(s) 95, 526, 858, 1041
ErhI CCWWGG 1 cut(s) 1047
FaeI CATG 7 cut(s) 106, 322, 374, 385, 691, 795, 1051
FaqI GGGAC 1 cut(s) 288
FatI CATG 7 cut(s) 102, 318, 370, 381, 687, 791, 1047
FblI GTMKAC 2 cut(s) 959, 1065
Fnu4HI GCNGC 6 cut(s) 65, 243, 273, 430, 780, 1005
FokI GGATG 5 cut(s) 185, 916, 949, 1002, 1377
FriOI GRGCYC 4 cut(s) 95, 526, 858, 1041
Fsp4HI GCNGC 6 cut(s) 65, 243, 273, 430, 780, 1005
FspBI CTAG 4 cut(s) 186, 837, 948, 1395
GluI GCNGC 6 cut(s) 65, 243, 273, 430, 780, 1005
GsaI CCCAGC 2 cut(s) 146, 1394
GsuI CTGGAG 2 cut(s) 333, 1179
HaeIII GGCC 7 cut(s) 58, 429, 445, 504, 524, 602, 1376
HapII CCGG 3 cut(s) 225, 285, 540
Hin1II CATG 7 cut(s) 106, 322, 374, 385, 691, 795, 1051
HinfI GANTC 8 cut(s) 12, 551, 676, 848, 926, 995, 1151, 1171
HpaII CCGG 3 cut(s) 225, 285, 540
HphI GGTGA 2 cut(s) 131, 1144
Hpy166II GTNNAC 3 cut(s) 592, 960, 1066
Hpy188I TCNGA 5 cut(s) 847, 853, 860, 1043, 1081
Hpy8I GTNNAC 3 cut(s) 592, 960, 1066
Hpy99I CGWCG 2 cut(s) 752, 1026
HpyAV CCTTC 4 cut(s) 225, 880, 1256, 1271
HpyCH4III ACNGT 1 cut(s) 1288
HpyCH4IV ACGT 2 cut(s) 45, 1057
HpyCH4V TGCA 6 cut(s) 275, 725, 773, 782, 1252, 1430
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 281, 521, 779, 1404
HpyF3I CTNAG 7 cut(s) 29, 60, 614, 852, 857, 1040, 1347
HpySE526I ACGT 2 cut(s) 45, 1057
Hsp92II CATG 7 cut(s) 106, 322, 374, 385, 691, 795, 1051
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Kzo9I GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
LguI GCTCTTC 1 cut(s) 808
LmnI GCTCC 3 cut(s) 182, 217, 1124
Lsp1109I GCAGC 5 cut(s) 76, 229, 259, 766, 991
LweI GCATC 6 cut(s) 1239, 1355, 1409, 1416, 1417, 1439
MaeI CTAG 4 cut(s) 186, 837, 948, 1395
MaeII ACGT 2 cut(s) 45, 1057
MaeIII GTNAC 4 cut(s) 415, 827, 901, 1132
MalI GATC 6 cut(s) 229, 562, 761, 1045, 1242, 1355
MboI GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
MboII GAAGA 7 cut(s) 397, 440, 489, 822, 825, 1196, 1378
MfeI CAATTG 1 cut(s) 200
MflI RGATCY 1 cut(s) 1043
MhlI GDGCHC 6 cut(s) 82, 95, 214, 526, 858, 1041
MluCI AATT 5 cut(s) 200, 261, 349, 738, 878
MlyI GAGTC 2 cut(s) 560, 935
Mph1103I ATGCAT 1 cut(s) 1432
MroNI GCCGGC 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 3 cut(s) 1110, 1179, 1272
MslI CAYNNNNRTG 1 cut(s) 1166
MspI CCGG 3 cut(s) 225, 285, 540
MspR9I CCNGG 1 cut(s) 324
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 5 cut(s) 64, 281, 521, 779, 1404
NaeI GCCGGC 1 cut(s) 286
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
NgoMIV GCCGGC 1 cut(s) 284
NheI GCTAGC 1 cut(s) 1394
NlaIII CATG 7 cut(s) 106, 322, 374, 385, 691, 795, 1051
NmuCI GTSAC 1 cut(s) 1132
NsiI ATGCAT 1 cut(s) 1432
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 923
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 808
PdiI GCCGGC 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 6 cut(s) 12, 676, 848, 995, 1151, 1171
PkrI GCNGC 6 cut(s) 66, 244, 274, 431, 781, 1006
PleI GAGTC 2 cut(s) 559, 934
PpsI GAGTC 2 cut(s) 559, 934
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 858
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 2 cut(s) 142, 1390
PspGI CCWGG 1 cut(s) 322
PspOMI GGGCCC 1 cut(s) 522
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 1043
RsaI GTAC 3 cut(s) 459, 573, 1268
RsaNI GTAC 3 cut(s) 458, 572, 1267
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 808
SaqAI TTAA 3 cut(s) 1110, 1179, 1272
SatI GCNGC 6 cut(s) 65, 243, 273, 430, 780, 1005
Sau3AI GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
SchI GAGTC 2 cut(s) 560, 935
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 6 cut(s) 82, 95, 214, 526, 858, 1041
SfaNI GCATC 6 cut(s) 1239, 1355, 1409, 1416, 1417, 1439
SfcI CTRYAG 1 cut(s) 273
Sfr274I CTCGAG 1 cut(s) 923
SinI GGWCC 7 cut(s) 222, 257, 302, 356, 398, 488, 542
SlaI CTCGAG 1 cut(s) 923
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 2 cut(s) 923, 1400
SmoI CTYRAG 2 cut(s) 923, 1400
SphI GCATGC 1 cut(s) 322
Sse9I AATT 5 cut(s) 200, 261, 349, 738, 878
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 1 cut(s) 430
SspI AATATT 1 cut(s) 663
SspMI CTAG 4 cut(s) 186, 837, 948, 1395
SstI GAGCTC 1 cut(s) 858
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 1 cut(s) 1288
TaiI ACGT 2 cut(s) 48, 1060
TaqI TCGA 3 cut(s) 529, 924, 985
TasI AATT 5 cut(s) 200, 261, 349, 738, 878
TatI WGTACW 1 cut(s) 571
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 6 cut(s) 12, 676, 848, 995, 1151, 1171
Tru1I TTAA 3 cut(s) 1110, 1179, 1272
Tru9I TTAA 3 cut(s) 1110, 1179, 1272
TscAI CASTG 3 cut(s) 366, 775, 1297
TseFI GTSAC 1 cut(s) 1132
TseI GCWGC 5 cut(s) 64, 242, 272, 779, 1004
Tsp45I GTSAC 1 cut(s) 1132
TspDTI ATGAA 7 cut(s) 398, 581, 624, 676, 688, 1197, 1332
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 3 cut(s) 366, 775, 1297
VpaK11BI GGWCC 7 cut(s) 222, 257, 302, 356, 398, 488, 542
XapI RAATTY 1 cut(s) 349
XceI RCATGY 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 923
XmiI GTMKAC 2 cut(s) 959, 1065
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 4 cut(s) 186, 837, 948, 1395
Zsp2I ATGCAT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.