Rmu_sc0014307.1_g000004

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014307.1
OG0001653
Physical Location & Seq
Forward (+)
18052 .. 20287
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014307.1_g000004.1.cds

Sequence Viewer

Length: 1266 bp
atgggttccagattcccatctcaccaactcagcaatggcctttacgtatcaggccggccagagcaaccaaaggaaaggactccaactatgagctcagttgccacaccttatactggtggagatatcaagaagtctggagaactaggaaagatgtttgatatccctacagatggctccaagtccagaaaatctggacctatcactggtggttcttctaggactggatcatttggaggtgctgcttcacattcaggaccaattcctaacgctgcaggtcgtgccggctatactacatcaggtcccatatctggaggcatgcctggttcagcttccgtaaagaagtcaaattctggaccactgaataagcatggggaacccatgaagaagtcatctggtcctcaatctggtggggtaacaccaactggccgccaaaactctgggcctcttccccccgtactccccaccacaggtctcattacttctggacccatttcttctggcccactaaatgcttctggggcccctcgaaaggtatccggtcctttggagtcaatgggatcaatgaaagtacaaggttcctctcttgttcacaatcaggccattactactcttagccaagatgatgagttttcgtttaggaaaaacttcccaaagccaatattatggtctttgattctgcttttcatgatggggttcatagctggtggctttattctcggtgcggtccataatccaattctcctcgtcgtggttgtgatccttttcagtgcagttgctgcactattcacatggaatacttattggggaagaagagctattataggttacattgctagttattcggattctgagctcagaactgccaagaatgggcaatttgtgaaggtgtcaggggtggttacttgtggtaatgtgcctctcgagtcatcctttcaaaaggttcctagatgtgtgtatacatccacaagtttatatgagtatcgaggatgggattcaaaacctgcgaaccctacacatcgtcgtttttcttgggggctcagatccatggaaagacgtgtggtagacttctacatctctgatttccaatctgggttgagagcattggttaagacaggctgtggagcaagggtgacaccttatgttgatgattcaattgtcattgatgtgaatcccgttaatgaagagttgtctccagagtttattaggtggttgagggagaggaacctttcaagtgatgatcgtgtgatgcagttgaaagaagggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

45.0

Weight (kDa)

9.85

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 263, 1009
AccI GTMKAC 2 cut(s) 956, 1062
AciI CCGC 2 cut(s) 427, 722
AclWI GGATC 4 cut(s) 232, 565, 751, 1035
AcoI YGGCCR 2 cut(s) 56, 424
AcsI RAATTY 1 cut(s) 346
AfaI GTAC 2 cut(s) 456, 570
AfiI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 935, 996, 1152, 1230, 1255
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 5 cut(s) 93, 329, 701, 815, 853
AluI AGCT 5 cut(s) 93, 329, 701, 815, 853
Alw21I GWGCWC 2 cut(s) 95, 855
Alw26I GTCTC 2 cut(s) 476, 1194
AlwI GGATC 4 cut(s) 232, 565, 751, 1035
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 920
AoxI GGCC 8 cut(s) 37, 52, 56, 424, 440, 499, 519, 597
ApaI GGGCCC 1 cut(s) 523
ApeKI GCWGC 3 cut(s) 239, 269, 776
ApoI RAATTY 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 14, 1141
AvaI CYCGRG 1 cut(s) 920
AvaII GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 724
BaeGI GKGCMC 1 cut(s) 523
BanII GRGCYC 4 cut(s) 95, 523, 855, 1038
Bbv12I GWGCWC 2 cut(s) 95, 855
BbvI GCAGC 3 cut(s) 226, 256, 763
BccI CCATC 4 cut(s) 25, 164, 682, 981
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 476, 1194
BfaI CTAG 4 cut(s) 143, 216, 834, 945
BfmI CTRYAG 2 cut(s) 165, 270
BfuAI ACCTGC 2 cut(s) 263, 1009
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 4 cut(s) 240, 270, 427, 777
BlsI GCNGC 4 cut(s) 241, 271, 428, 778
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 724
BmeT110I CYCGRG 1 cut(s) 920
BmgBI CACGTC 1 cut(s) 1055
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 1236
BpmI CTGGAG 3 cut(s) 156, 330, 1176
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 2 cut(s) 16, 1167
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 1044
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
Bse118I RCCGGY 2 cut(s) 54, 281
Bse1I ACTGG 4 cut(s) 118, 208, 226, 427
Bse3DI GCAATG 2 cut(s) 40, 828
Bse8I GATNNNNATC 2 cut(s) 16, 1167
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 1044
BseGI GGATG 3 cut(s) 926, 959, 992
BseJI GATNNNNATC 2 cut(s) 16, 1167
BseLI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
BseMI GCAATG 2 cut(s) 40, 828
BseMII CTCAG 5 cut(s) 43, 108, 840, 868, 1051
BseNI ACTGG 4 cut(s) 118, 208, 226, 427
BseRI GAGGAG 1 cut(s) 731
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 3 cut(s) 226, 256, 763
BsgI GTGCAG 2 cut(s) 762, 789
BshFI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 521, 599
BsiHKAI GWGCWC 2 cut(s) 95, 855
BsiHKCI CYCGRG 1 cut(s) 920
BsiSI CCGG 3 cut(s) 55, 282, 537
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
BsmAI GTCTC 2 cut(s) 476, 1194
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 521, 599
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 920
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 4 cut(s) 95, 523, 855, 1038
Bsp143I GATC 5 cut(s) 224, 557, 756, 1040, 1237
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 2 cut(s) 427, 722
BspANI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 521, 599
BspCNI CTCAG 5 cut(s) 42, 107, 841, 867, 1050
BspHI TCATGA 1 cut(s) 684
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 2 cut(s) 263, 1009
BspPI GGATC 4 cut(s) 232, 565, 751, 1035
BspQI GCTCTTC 1 cut(s) 805
BspTNI GGTCTC 1 cut(s) 476
BsrDI GCAATG 2 cut(s) 40, 828
BsrFI RCCGGY 2 cut(s) 54, 281
BsrI ACTGG 4 cut(s) 118, 208, 226, 427
BssAI RCCGGY 2 cut(s) 54, 281
BssECI CCNNGG 1 cut(s) 1044
BssMI GATC 5 cut(s) 224, 557, 756, 1040, 1237
BssNAI GTATAC 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 1044
Bst1107I GTATAC 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 321
Bst6I CTCTTC 3 cut(s) 450, 805, 1176
BstAPI GCANNNNNTGC 2 cut(s) 278, 776
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 3 cut(s) 56, 283, 317
BstDEI CTNAG 6 cut(s) 29, 94, 611, 849, 854, 1037
BstDSI CCRYGG 1 cut(s) 1044
BstF5I GGATG 3 cut(s) 926, 959, 992
BstKTI GATC 5 cut(s) 227, 560, 759, 1043, 1240
BstMAI GTCTC 2 cut(s) 476, 1194
BstMBI GATC 5 cut(s) 224, 557, 756, 1040, 1237
BstMWI GCNNNNNNNGC 3 cut(s) 278, 518, 776
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 2 cut(s) 165, 270
BstSLI GKGCMC 1 cut(s) 523
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 226, 256, 763
BstX2I RGATCY 1 cut(s) 1040
BstXI CCANNNNNNTGG 2 cut(s) 437, 663
BstYI RGATCY 1 cut(s) 1040
BstZ17I GTATAC 1 cut(s) 957
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 521, 599
BtgI CCRYGG 1 cut(s) 1044
BtrI CACGTC 1 cut(s) 1055
BtsCI GGATG 3 cut(s) 926, 959, 992
BtsIMutI CAGTG 3 cut(s) 201, 356, 772
BveI ACCTGC 2 cut(s) 263, 1009
Cac8I GCNNGC 3 cut(s) 56, 283, 317
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 684
Cfr10I RCCGGY 2 cut(s) 54, 281
Csp6I GTAC 2 cut(s) 455, 569
CviAII CATG 6 cut(s) 316, 368, 379, 685, 789, 1045
CviQI GTAC 2 cut(s) 455, 569
DdeI CTNAG 6 cut(s) 29, 94, 611, 849, 854, 1037
DpnI GATC 5 cut(s) 226, 559, 758, 1042, 1239
DpnII GATC 5 cut(s) 224, 557, 756, 1040, 1237
EaeI YGGCCR 2 cut(s) 56, 424
Eam1104I CTCTTC 3 cut(s) 450, 805, 1176
EarI CTCTTC 3 cut(s) 450, 805, 1176
Ecl136II GAGCTC 2 cut(s) 93, 853
Eco105I TACGTA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 1044
Eco24I GRGCYC 4 cut(s) 95, 523, 855, 1038
Eco31I GGTCTC 1 cut(s) 476
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 724
Eco53kI GAGCTC 2 cut(s) 93, 853
Eco88I CYCGRG 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 93, 853
EcoO109I RGGNCCY 3 cut(s) 299, 519, 520
EcoRII CCWGG 1 cut(s) 319
EcoRV GATATC 2 cut(s) 124, 160
EcoT14I CCWWGG 1 cut(s) 1044
EcoT38I GRGCYC 4 cut(s) 95, 523, 855, 1038
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
FaqI GGGAC 1 cut(s) 285
FatI CATG 6 cut(s) 315, 367, 378, 684, 788, 1044
FblI GTMKAC 2 cut(s) 956, 1062
Fnu4HI GCNGC 4 cut(s) 240, 270, 427, 777
FokI GGATG 3 cut(s) 913, 946, 999
FriOI GRGCYC 4 cut(s) 95, 523, 855, 1038
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 4 cut(s) 240, 270, 427, 777
FspBI CTAG 4 cut(s) 143, 216, 834, 945
GluI GCNGC 4 cut(s) 240, 270, 427, 777
GsuI CTGGAG 3 cut(s) 156, 330, 1176
HaeIII GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 521, 599
HapII CCGG 3 cut(s) 55, 282, 537
Hin1II CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
HinfI GANTC 9 cut(s) 12, 79, 548, 673, 845, 923, 992, 1148, 1168
HpaII CCGG 3 cut(s) 55, 282, 537
HphI GGTGA 2 cut(s) 14, 1141
Hpy166II GTNNAC 3 cut(s) 589, 957, 1063
Hpy188I TCNGA 5 cut(s) 844, 850, 857, 1040, 1078
Hpy8I GTNNAC 3 cut(s) 589, 957, 1063
Hpy99I CGWCG 2 cut(s) 749, 1023
HpyAV CCTTC 2 cut(s) 877, 1253
HpyCH4IV ACGT 2 cut(s) 45, 1054
HpyCH4V TGCA 4 cut(s) 272, 770, 779, 1249
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 518, 776
HpyF3I CTNAG 6 cut(s) 29, 94, 611, 849, 854, 1037
HpySE526I ACGT 2 cut(s) 45, 1054
Hsp92II CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
KroI GCCGGC 2 cut(s) 54, 281
KroNI GCCGGC 2 cut(s) 56, 283
Kzo9I GATC 5 cut(s) 224, 557, 756, 1040, 1237
LguI GCTCTTC 1 cut(s) 805
LmnI GCTCC 2 cut(s) 179, 1121
Lsp1109I GCAGC 3 cut(s) 226, 256, 763
LweI GCATC 1 cut(s) 1236
MaeI CTAG 4 cut(s) 143, 216, 834, 945
MaeII ACGT 2 cut(s) 45, 1054
MaeIII GTNAC 4 cut(s) 412, 824, 898, 1129
MalI GATC 5 cut(s) 226, 559, 758, 1042, 1239
MboI GATC 5 cut(s) 224, 557, 756, 1040, 1237
MboII GAAGA 7 cut(s) 204, 394, 437, 486, 819, 822, 1193
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 1 cut(s) 1040
MhlI GDGCHC 4 cut(s) 95, 523, 855, 1038
MluCI AATT 5 cut(s) 258, 346, 735, 875, 1152
MlyI GAGTC 3 cut(s) 73, 557, 932
MmeI TCCRAC 1 cut(s) 107
MroNI GCCGGC 2 cut(s) 54, 281
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 2 cut(s) 1107, 1176
MslI CAYNNNNRTG 1 cut(s) 1163
MspI CCGG 3 cut(s) 55, 282, 537
MspR9I CCNGG 1 cut(s) 321
MunI CAATTG 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 3 cut(s) 278, 518, 776
NaeI GCCGGC 2 cut(s) 56, 283
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 5 cut(s) 224, 557, 756, 1040, 1237
NgoMIV GCCGGC 2 cut(s) 54, 281
NlaIII CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
NmuCI GTSAC 1 cut(s) 1129
NspI RCATGY 1 cut(s) 319
PaeI GCATGC 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 920
PagI TCATGA 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 805
PdiI GCCGGC 2 cut(s) 56, 283
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 6 cut(s) 12, 673, 845, 992, 1148, 1168
PkrI GCNGC 4 cut(s) 241, 271, 428, 778
PleI GAGTC 3 cut(s) 73, 556, 931
PpsI GAGTC 3 cut(s) 73, 556, 931
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 2 cut(s) 95, 855
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspOMI GGGCCC 1 cut(s) 519
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 1 cut(s) 1040
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 2 cut(s) 456, 570
RsaNI GTAC 2 cut(s) 455, 569
RseI CAYNNNNRTG 1 cut(s) 1163
SacI GAGCTC 2 cut(s) 95, 855
SapI GCTCTTC 1 cut(s) 805
SaqAI TTAA 2 cut(s) 1107, 1176
SatI GCNGC 4 cut(s) 240, 270, 427, 777
Sau3AI GATC 5 cut(s) 224, 557, 756, 1040, 1237
SchI GAGTC 3 cut(s) 73, 557, 932
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 4 cut(s) 95, 523, 855, 1038
SfaNI GCATC 1 cut(s) 1236
SfcI CTRYAG 2 cut(s) 165, 270
Sfr274I CTCGAG 1 cut(s) 920
SinI GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 724
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 319
Sse9I AATT 5 cut(s) 258, 346, 735, 875, 1152
SsiI CCGC 2 cut(s) 427, 722
SspI AATATT 1 cut(s) 660
SspMI CTAG 4 cut(s) 143, 216, 834, 945
SstI GAGCTC 2 cut(s) 95, 855
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 1044
TaiI ACGT 2 cut(s) 48, 1057
TaqI TCGA 3 cut(s) 526, 921, 982
TasI AATT 5 cut(s) 258, 346, 735, 875, 1152
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 6 cut(s) 12, 673, 845, 992, 1148, 1168
Tru1I TTAA 2 cut(s) 1107, 1176
Tru9I TTAA 2 cut(s) 1107, 1176
TscAI CASTG 3 cut(s) 208, 363, 772
TseFI GTSAC 1 cut(s) 1129
TseI GCWGC 3 cut(s) 239, 269, 776
Tsp45I GTSAC 1 cut(s) 1129
TspDTI ATGAA 5 cut(s) 395, 578, 673, 685, 1194
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 3 cut(s) 208, 363, 772
VpaK11BI GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 724
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 2 cut(s) 956, 1062
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 4 cut(s) 143, 216, 834, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.