Rh2AG474300

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001653
Physical Location & Seq
Forward (+)
69332297 .. 69335275
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG474300.1

Sequence Viewer

Length: 1455 bp
ATGGGTTCCAGATTCCCATCTCACCAACTCAGCAATGGCCTTTACGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCCACACCTTATACTGGTGGAGATATCAAGAAGTCTGGAGAACTAGGAAAGATGTTTGATATCCCTACAGATGGCTCCAAGTCCAGAAAATCTGGACCTATCACTGGTGGTTCTTCTAGGACTGGATCATTTGGAGGTGCTGCTTCACATTCAGGACCAATTCCTAACGCTGCAGGTCGTGCCGGCTATACTACATCAGGTCCCATATCTGGAGGCATGCCTGGTTCAGCTTCCGTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCCATGAAGAAGTCATCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCTGGGCCTCTTCCCCCCGTACTCCCCACCACAGGTCTCATTACTTCTGGACCCATTTCTTCTGGCCCACTAAATGCTTCTGGGGCCCCTCGAAAGGTATCCGGTCCTTTGGAGTCAATGGGATCAATGAAAGTACAAGGTTCCTCTCTTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGATGAGTTTTCATTTAGGAAAAACTTCCCAAAGCCAATATTATGGTCTTTGATTCTGCTTTTCATGATGGGGTTCATAGCTGGTGGCTTTATTCTCGGTGCAGTCCATAATCCAATTCTCCTCGTCGTGGTTGTGATCCTTTTCAGTGCAGTTGCTGCACTATTCACATGGAATACTTATTGGGGAAGAAGAGCTATTATAGGTTACATTGCTAGTTATTCGGATTCTGAGCTCAGAACTGCCAAGAATGGGCAATTTGTGAAGGTGTCAGGGGTGGTTACTTGTGGCAATGTGCCTCTCGAGTCATCCTTTCAAAAGGTTCCTAGATGTGTGTATACATCCACAAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCGTCGTTTTTCTTGGGGGCTCAGATCCATGGAAAGACGTGTGGTAGACTTCTACATCTCTGATTTCCAATCTGGGTTGAGAGCATTGGTTAAGACAGGCTGTGGAGCAAGGGTGACACCTTATGTTGATGATTCAATCGTCATTGATGTGAATCCCGTTAATGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGAGGGAGAGGAACCTTTCAAGTGATGATCGTGTGATGCAGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTCAGTGTAATGGGGGTCGTCCAGAGAAATGAAAATGTGTTGATGATTGTTCCTCCACCTGAGCCGATCACAACGGGATGCCAATGGGCCAAATGTATCTTCCCAGCTAGCCTTGAGGGCATCGTTTTGAGATGCGATGATGCATCAAAGAATGATGTCATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

51.78

Weight (kDa)

9.54

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 956, 1062
AciI CCGC 1 cut(s) 427
AclWI GGATC 4 cut(s) 232, 565, 751, 1035
AcoI YGGCCR 2 cut(s) 56, 424
AcsI RAATTY 1 cut(s) 346
AfaI GTAC 3 cut(s) 456, 570, 1265
AfiI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 935, 996, 1152, 1230, 1255
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
AluBI AGCT 7 cut(s) 93, 329, 701, 815, 853, 1001, 1391
AluI AGCT 7 cut(s) 93, 329, 701, 815, 853, 1001, 1391
Alw21I GWGCWC 2 cut(s) 95, 855
Alw26I GTCTC 2 cut(s) 476, 1194
AlwI GGATC 4 cut(s) 232, 565, 751, 1035
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 920
AoxI GGCC 9 cut(s) 37, 52, 56, 424, 440, 499, 519, 597, 1371
ApaI GGGCCC 1 cut(s) 523
ApeKI GCWGC 4 cut(s) 239, 269, 776, 1001
ApoI RAATTY 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 14, 1141
AsuNHI GCTAGC 1 cut(s) 1391
AvaI CYCGRG 1 cut(s) 920
AvaII GGWCC 7 cut(s) 194, 254, 299, 353, 395, 485, 539
BaeGI GKGCMC 1 cut(s) 523
BanII GRGCYC 4 cut(s) 95, 523, 855, 1038
Bbv12I GWGCWC 2 cut(s) 95, 855
BbvI GCAGC 4 cut(s) 226, 256, 763, 988
BccI CCATC 4 cut(s) 25, 164, 682, 981
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 476, 1194
BfaI CTAG 5 cut(s) 143, 216, 834, 945, 1392
BfmI CTRYAG 2 cut(s) 165, 270
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 1401
BisI GCNGC 5 cut(s) 240, 270, 427, 777, 1002
BlsI GCNGC 5 cut(s) 241, 271, 428, 778, 1003
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 7 cut(s) 194, 254, 299, 353, 395, 485, 539
BmeT110I CYCGRG 1 cut(s) 920
BmgBI CACGTC 1 cut(s) 1055
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 6 cut(s) 1236, 1352, 1406, 1413, 1414, 1436
BmtI GCTAGC 1 cut(s) 1395
BpmI CTGGAG 3 cut(s) 156, 330, 1176
Bpu10I CCTNAGC 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 1418
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 2 cut(s) 16, 1167
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 1044
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
Bse118I RCCGGY 2 cut(s) 54, 281
Bse1I ACTGG 5 cut(s) 118, 208, 226, 427, 1448
Bse3DI GCAATG 3 cut(s) 40, 828, 916
Bse8I GATNNNNATC 2 cut(s) 16, 1167
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 1044
BseGI GGATG 4 cut(s) 926, 959, 992, 1367
BseJI GATNNNNATC 2 cut(s) 16, 1167
BseLI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
BseMI GCAATG 3 cut(s) 40, 828, 916
BseMII CTCAG 6 cut(s) 43, 108, 840, 868, 1051, 1335
BseNI ACTGG 5 cut(s) 118, 208, 226, 427, 1448
BseRI GAGGAG 1 cut(s) 731
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 4 cut(s) 226, 256, 763, 988
BseYI CCCAGC 1 cut(s) 1387
BsgI GTGCAG 3 cut(s) 741, 762, 789
BshFI GGCC 9 cut(s) 39, 54, 58, 426, 442, 501, 521, 599, 1373
BsiHKAI GWGCWC 2 cut(s) 95, 855
BsiHKCI CYCGRG 1 cut(s) 920
BsiSI CCGG 3 cut(s) 55, 282, 537
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 748, 870
BsmAI GTCTC 2 cut(s) 476, 1194
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 9 cut(s) 39, 54, 58, 426, 442, 501, 521, 599, 1373
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 920
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 4 cut(s) 95, 523, 855, 1038
Bsp143I GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 1 cut(s) 427
BspANI GGCC 9 cut(s) 39, 54, 58, 426, 442, 501, 521, 599, 1373
BspCNI CTCAG 6 cut(s) 42, 107, 841, 867, 1050, 1336
BspHI TCATGA 1 cut(s) 684
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 263
BspOI GCTAGC 1 cut(s) 1395
BspPI GGATC 4 cut(s) 232, 565, 751, 1035
BspQI GCTCTTC 1 cut(s) 805
BspTNI GGTCTC 1 cut(s) 476
BsrDI GCAATG 3 cut(s) 40, 828, 916
BsrFI RCCGGY 2 cut(s) 54, 281
BsrI ACTGG 5 cut(s) 118, 208, 226, 427, 1448
BssAI RCCGGY 2 cut(s) 54, 281
BssECI CCNNGG 1 cut(s) 1044
BssMI GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
BssNAI GTATAC 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 1044
Bst1107I GTATAC 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 1285
Bst6I CTCTTC 3 cut(s) 450, 805, 1176
BstAPI GCANNNNNTGC 2 cut(s) 278, 776
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 56, 283, 317, 1393
BstDEI CTNAG 7 cut(s) 29, 94, 611, 849, 854, 1037, 1344
BstDSI CCRYGG 1 cut(s) 1044
BstF5I GGATG 4 cut(s) 926, 959, 992, 1367
BstKTI GATC 6 cut(s) 227, 560, 759, 1043, 1240, 1353
BstMAI GTCTC 2 cut(s) 476, 1194
BstMBI GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
BstMWI GCNNNNNNNGC 4 cut(s) 278, 518, 776, 1401
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 2 cut(s) 165, 270
BstSLI GKGCMC 1 cut(s) 523
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 4 cut(s) 226, 256, 763, 988
BstX2I RGATCY 1 cut(s) 1040
BstXI CCANNNNNNTGG 2 cut(s) 437, 663
BstYI RGATCY 1 cut(s) 1040
BstZ17I GTATAC 1 cut(s) 957
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 9 cut(s) 39, 54, 58, 426, 442, 501, 521, 599, 1373
BtgI CCRYGG 1 cut(s) 1044
BtgZI GCGATG 1 cut(s) 1434
BtrI CACGTC 1 cut(s) 1055
BtsCI GGATG 4 cut(s) 926, 959, 992, 1367
BtsIMutI CAGTG 4 cut(s) 201, 356, 772, 1294
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 4 cut(s) 56, 283, 317, 1393
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 684
Cfr10I RCCGGY 2 cut(s) 54, 281
Csp6I GTAC 3 cut(s) 455, 569, 1264
CviAII CATG 6 cut(s) 316, 368, 379, 685, 789, 1045
CviQI GTAC 3 cut(s) 455, 569, 1264
DdeI CTNAG 7 cut(s) 29, 94, 611, 849, 854, 1037, 1344
DpnI GATC 6 cut(s) 226, 559, 758, 1042, 1239, 1352
DpnII GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
EaeI YGGCCR 2 cut(s) 56, 424
Eam1104I CTCTTC 3 cut(s) 450, 805, 1176
EarI CTCTTC 3 cut(s) 450, 805, 1176
Ecl136II GAGCTC 2 cut(s) 93, 853
Eco105I TACGTA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 1044
Eco24I GRGCYC 4 cut(s) 95, 523, 855, 1038
Eco31I GGTCTC 1 cut(s) 476
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 7 cut(s) 194, 254, 299, 353, 395, 485, 539
Eco53kI GAGCTC 2 cut(s) 93, 853
Eco88I CYCGRG 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 93, 853
EcoO109I RGGNCCY 3 cut(s) 299, 519, 520
EcoRII CCWGG 1 cut(s) 319
EcoRV GATATC 2 cut(s) 124, 160
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 1 cut(s) 1429
EcoT38I GRGCYC 4 cut(s) 95, 523, 855, 1038
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
FaqI GGGAC 1 cut(s) 285
FatI CATG 6 cut(s) 315, 367, 378, 684, 788, 1044
FblI GTMKAC 2 cut(s) 956, 1062
Fnu4HI GCNGC 5 cut(s) 240, 270, 427, 777, 1002
FokI GGATG 4 cut(s) 913, 946, 999, 1374
FriOI GRGCYC 4 cut(s) 95, 523, 855, 1038
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 5 cut(s) 240, 270, 427, 777, 1002
FspBI CTAG 5 cut(s) 143, 216, 834, 945, 1392
GluI GCNGC 5 cut(s) 240, 270, 427, 777, 1002
GsaI CCCAGC 1 cut(s) 1391
GsuI CTGGAG 3 cut(s) 156, 330, 1176
HaeIII GGCC 9 cut(s) 39, 54, 58, 426, 442, 501, 521, 599, 1373
HapII CCGG 3 cut(s) 55, 282, 537
Hin1II CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
HinfI GANTC 9 cut(s) 12, 79, 548, 673, 845, 923, 992, 1148, 1168
HpaII CCGG 3 cut(s) 55, 282, 537
HphI GGTGA 2 cut(s) 14, 1141
Hpy166II GTNNAC 3 cut(s) 589, 957, 1063
Hpy188I TCNGA 5 cut(s) 844, 850, 857, 1040, 1078
Hpy8I GTNNAC 3 cut(s) 589, 957, 1063
Hpy99I CGWCG 2 cut(s) 749, 1023
HpyAV CCTTC 3 cut(s) 877, 1253, 1268
HpyCH4III ACNGT 1 cut(s) 1285
HpyCH4IV ACGT 2 cut(s) 45, 1054
HpyCH4V TGCA 6 cut(s) 272, 722, 770, 779, 1249, 1427
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 518, 776, 1401
HpyF3I CTNAG 7 cut(s) 29, 94, 611, 849, 854, 1037, 1344
HpySE526I ACGT 2 cut(s) 45, 1054
Hsp92II CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
KroI GCCGGC 2 cut(s) 54, 281
KroNI GCCGGC 2 cut(s) 56, 283
Kzo9I GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
LguI GCTCTTC 1 cut(s) 805
LmnI GCTCC 2 cut(s) 179, 1121
Lsp1109I GCAGC 4 cut(s) 226, 256, 763, 988
LweI GCATC 6 cut(s) 1236, 1352, 1406, 1413, 1414, 1436
MaeI CTAG 5 cut(s) 143, 216, 834, 945, 1392
MaeII ACGT 2 cut(s) 45, 1054
MaeIII GTNAC 4 cut(s) 412, 824, 898, 1129
MalI GATC 6 cut(s) 226, 559, 758, 1042, 1239, 1352
MboI GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
MboII GAAGA 8 cut(s) 204, 394, 437, 486, 819, 822, 1193, 1375
MflI RGATCY 1 cut(s) 1040
MhlI GDGCHC 4 cut(s) 95, 523, 855, 1038
MluCI AATT 4 cut(s) 258, 346, 735, 875
MlyI GAGTC 3 cut(s) 73, 557, 932
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1429
MroNI GCCGGC 2 cut(s) 54, 281
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 3 cut(s) 1107, 1176, 1269
MslI CAYNNNNRTG 1 cut(s) 1163
MspI CCGG 3 cut(s) 55, 282, 537
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 4 cut(s) 278, 518, 776, 1401
NaeI GCCGGC 2 cut(s) 56, 283
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
NgoMIV GCCGGC 2 cut(s) 54, 281
NheI GCTAGC 1 cut(s) 1391
NlaIII CATG 6 cut(s) 319, 371, 382, 688, 792, 1048
NmuCI GTSAC 1 cut(s) 1129
NsiI ATGCAT 1 cut(s) 1429
NspI RCATGY 1 cut(s) 319
PaeI GCATGC 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 920
PagI TCATGA 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 805
PdiI GCCGGC 2 cut(s) 56, 283
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 6 cut(s) 12, 673, 845, 992, 1148, 1168
PkrI GCNGC 5 cut(s) 241, 271, 428, 778, 1003
PleI GAGTC 3 cut(s) 73, 556, 931
PpsI GAGTC 3 cut(s) 73, 556, 931
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 2 cut(s) 95, 855
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 1387
PspGI CCWGG 1 cut(s) 319
PspOMI GGGCCC 1 cut(s) 519
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 1 cut(s) 1040
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 3 cut(s) 456, 570, 1265
RsaNI GTAC 3 cut(s) 455, 569, 1264
RseI CAYNNNNRTG 1 cut(s) 1163
SacI GAGCTC 2 cut(s) 95, 855
SapI GCTCTTC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 1107, 1176, 1269
SatI GCNGC 5 cut(s) 240, 270, 427, 777, 1002
Sau3AI GATC 6 cut(s) 224, 557, 756, 1040, 1237, 1350
SchI GAGTC 3 cut(s) 73, 557, 932
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 4 cut(s) 95, 523, 855, 1038
SfaNI GCATC 6 cut(s) 1236, 1352, 1406, 1413, 1414, 1436
SfcI CTRYAG 2 cut(s) 165, 270
Sfr274I CTCGAG 1 cut(s) 920
SinI GGWCC 7 cut(s) 194, 254, 299, 353, 395, 485, 539
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 2 cut(s) 920, 1397
SmoI CTYRAG 2 cut(s) 920, 1397
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 319
Sse9I AATT 4 cut(s) 258, 346, 735, 875
SsiI CCGC 1 cut(s) 427
SspI AATATT 1 cut(s) 660
SspMI CTAG 5 cut(s) 143, 216, 834, 945, 1392
SstI GAGCTC 2 cut(s) 95, 855
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 1 cut(s) 1285
TaiI ACGT 2 cut(s) 48, 1057
TaqI TCGA 3 cut(s) 526, 921, 982
TasI AATT 4 cut(s) 258, 346, 735, 875
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 6 cut(s) 12, 673, 845, 992, 1148, 1168
Tru1I TTAA 3 cut(s) 1107, 1176, 1269
Tru9I TTAA 3 cut(s) 1107, 1176, 1269
TscAI CASTG 4 cut(s) 208, 363, 772, 1294
TseFI GTSAC 1 cut(s) 1129
TseI GCWGC 4 cut(s) 239, 269, 776, 1001
Tsp45I GTSAC 1 cut(s) 1129
TspDTI ATGAA 7 cut(s) 395, 578, 621, 673, 685, 1194, 1329
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 4 cut(s) 208, 363, 772, 1294
VpaK11BI GGWCC 7 cut(s) 194, 254, 299, 353, 395, 485, 539
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 2 cut(s) 956, 1062
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 5 cut(s) 143, 216, 834, 945, 1392
Zsp2I ATGCAT 1 cut(s) 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.