FvH4_6g37090

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001653
Physical Location & Seq
Forward (+)
29155717 .. 29160631
4915 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37090.t3

Sequence Viewer

Length: 1446 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGTCTCTACGTATCAGGCAGGCCTGAGCAGCCAAAAGAAAGACCACCTACAATGGGCTCAACTGCCATGCCCTATACTGGTGGTGATATCAAGAAGTCAGGAGAGCTGGGTAAAATGTTTGATATCCCTTCAATGGATGGCTCCAAATCCAGGAAATCTGGACAATTGACCGGTGCTCCTTCAAGGACCGGATCATTTGGAGGTGCTGCTTCACATTCAGGTCCTATTCCTAATGCTGCTGCTCGGTCTATCTATACCACGTCGGGTCCTGTAACTTCTGGTGGCATGCCTGGTTCAGTTTCAATGAAGAAAACTAATTCTGGACCACTCAATAAGCATGGAGAACCTTTGAAGAAGTCCTCTGGGCCTCAATCTGGTGGAGTGACACGCCAAAATTCAGGCCAAATACCAGTACTTCCTACAACAGGTCTCATCACATCTGGTCCGATTTCTTCAGGACCACTAAATTCGTCTGGGGCACCTAGAAAGGTTTCTGGACCTTTAGAGTCTATGGGATCAATGAAATTACAAGGTTCTGCAATTGTTCACAATCAAGCAGTGACTACTCTTGGCCAGGATGATTATTCTTTCAGAAAGAACTTTCCAAAGACCATACTATGGTCTGTGATACTGATATTTGTGATGGGATTCATCGCTGGTGGTTTTATTCTTGGAGCAGTCCACAATCCCATACTCCTAGCTGTTGTGGCGATTCTCTTTGCTGGTGTTGCTGCTTTATTCACTTGGAATACTTGTTGGGGAAGAAAAGCTACTACAAATTTCATTGCTCGCTATCCTGATGCTGAGCTGAGAACCGCTAAAAATGGGCAATATGTGAAGGTCTCCGGGGTGGTTACCTGTGGTAATGTGCCCCTTGAATCATCATTTCGGAGAGTTCCTAGATGTGTATATACATCTACGAGTTTGTATGAGTACCGAGGATGGGATTCAAAACCAGCAAATCCTACACATCGGCGATTTACATGGGGACTTAGATCATCAGAGAGGCATGTAGTGGACTTCTACATCTCTGATTTCCAGTCTGGTTTGAGAGCTTTGGTTAAGACTGGCTATGGAACCAAAGTTACTCCTTATGTGGATGAGTCTGTAATTATTGATGTGAACCCATCAAACAAAGACATGTCCCCAGATTTTGTGAGGTGGTTGGCAGAGAGGAATCTTTCAAGTGACGAGCGAACAATGCGATTGAAAGAAGGGTACATCAAGGAAGGTAGCACGGTTAGTGTAATGGGAGTTGTCCAGAGAAATGACAACGTGCTAATGATCGTTCCTCCACCTGAGCCCTTGACAACTGGATGCCAATGGAGCAGTTGTATCTTTCCAGCTAGCCTTGAGGGTGTTGTTTTGAGATGTGAAGACTCATCAAAAAATGATGCCATACCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

482

Amino Acids

51.58

Weight (kDa)

9.66

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 654
AciI CCGC 1 cut(s) 852
AclWI GGATC 2 cut(s) 235, 559
AcoI YGGCCR 1 cut(s) 607
AcsI RAATTY 3 cut(s) 430, 502, 814
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 3 cut(s) 450, 971, 1256
AfiI CCNNNNNNNGG 8 cut(s) 89, 113, 169, 186, 410, 461, 654, 979
AflIII ACRYGT 1 cut(s) 1176
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 8 cut(s) 168, 219, 339, 388, 914, 987, 1221, 1246
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 3 cut(s) 185, 325, 609
AluBI AGCT 7 cut(s) 28, 142, 737, 806, 844, 1091, 1382
AluI AGCT 7 cut(s) 28, 142, 737, 806, 844, 1091, 1382
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 3 cut(s) 44, 470, 883
AlwI GGATC 2 cut(s) 235, 559
AoxI GGCC 4 cut(s) 56, 401, 436, 607
ApeKI GCWGC 5 cut(s) 64, 242, 272, 275, 767
ApoI RAATTY 3 cut(s) 430, 502, 814
AsiGI ACCGGT 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 526
AspS9I GGNCC 8 cut(s) 222, 257, 302, 359, 401, 479, 494, 533
AsuC2I CCSGG 1 cut(s) 883
AsuHPI GGTGA 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 1382
AvaII GGWCC 7 cut(s) 222, 257, 302, 359, 479, 494, 533
BaeGI GKGCMC 2 cut(s) 517, 909
BalI TGGCCA 1 cut(s) 609
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 2 cut(s) 95, 1341
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
BbsI GAAGAC 1 cut(s) 1419
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 5 cut(s) 76, 229, 259, 262, 754
BccI CCATC 5 cut(s) 25, 167, 673, 972, 1171
BciT130I CCWGG 3 cut(s) 187, 327, 611
BcnI CCSGG 1 cut(s) 883
BcoDI GTCTC 3 cut(s) 44, 470, 883
BfaI CTAG 4 cut(s) 519, 734, 936, 1383
BisI GCNGC 5 cut(s) 65, 243, 273, 276, 768
BlpI GCTNAGC 2 cut(s) 29, 840
BlsI GCNGC 5 cut(s) 66, 244, 274, 277, 769
BmcAI AGTACT 1 cut(s) 450
Bme1390I CCNGG 4 cut(s) 187, 327, 611, 883
Bme18I GGWCC 7 cut(s) 222, 257, 302, 359, 479, 494, 533
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 8 cut(s) 222, 257, 302, 359, 401, 479, 494, 533
BmiI GGNNCC 5 cut(s) 7, 178, 303, 516, 1114
BmrFI CCNGG 4 cut(s) 187, 327, 611, 883
BmsI GCATC 3 cut(s) 826, 1343, 1420
BmtI GCTAGC 1 cut(s) 1386
BpiI GAAGAC 1 cut(s) 1419
Bpu10I CCTNAGC 2 cut(s) 60, 1335
Bpu1102I GCTNAGC 2 cut(s) 29, 840
BpuEI CTTGAG 1 cut(s) 1409
BpuMI CCSGG 1 cut(s) 883
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 16
BsaI GGTCTC 3 cut(s) 44, 470, 883
BsaJI CCNNGG 2 cut(s) 882, 973
BsaWI WCCGGW 2 cut(s) 206, 224
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 1354, 1384
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 113, 169, 186, 410, 461, 654, 979
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 5 cut(s) 118, 446, 1075, 1108, 1354
Bse3DI GCAATG 2 cut(s) 40, 819
Bse8I GATNNNNATC 1 cut(s) 16
BseBI CCWGG 3 cut(s) 187, 327, 611
BseDI CCNNGG 2 cut(s) 882, 973
BseGI GGATG 5 cut(s) 178, 619, 983, 1141, 1358
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 8 cut(s) 89, 113, 169, 186, 410, 461, 654, 979
BseMI GCAATG 2 cut(s) 40, 819
BseMII CTCAG 5 cut(s) 43, 51, 831, 836, 1326
BseNI ACTGG 5 cut(s) 118, 446, 1075, 1108, 1354
BseSI GKGCMC 2 cut(s) 517, 909
BseXI GCAGC 5 cut(s) 76, 229, 259, 262, 754
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 4 cut(s) 58, 403, 438, 609
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 3 cut(s) 207, 225, 882
BslFI GGGAC 2 cut(s) 1038, 1165
BslI CCNNNNNNNGG 8 cut(s) 89, 113, 169, 186, 410, 461, 654, 979
BsmAI GTCTC 3 cut(s) 44, 470, 883
BsmFI GGGAC 2 cut(s) 1038, 1165
BsnI GGCC 4 cut(s) 58, 403, 438, 609
Bso31I GGTCTC 3 cut(s) 44, 470, 883
Bsp1286I GDGCHC 5 cut(s) 95, 214, 517, 909, 1341
Bsp143I GATC 4 cut(s) 227, 551, 1031, 1320
Bsp1720I GCTNAGC 2 cut(s) 29, 840
BspACI CCGC 1 cut(s) 852
BspANI GGCC 4 cut(s) 58, 403, 438, 609
BspCNI CTCAG 5 cut(s) 42, 52, 832, 837, 1327
BspLI GGNNCC 5 cut(s) 7, 178, 303, 516, 1114
BspOI GCTAGC 1 cut(s) 1386
BspPI GGATC 2 cut(s) 235, 559
BspT107I GGYRCC 1 cut(s) 514
BspTNI GGTCTC 3 cut(s) 44, 470, 883
BsrDI GCAATG 2 cut(s) 40, 819
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 5 cut(s) 118, 446, 1075, 1108, 1354
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 882, 973
BssMI GATC 4 cut(s) 227, 551, 1031, 1320
Bst2UI CCWGG 3 cut(s) 187, 327, 611
Bst4CI ACNGT 1 cut(s) 1276
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 56, 323, 826, 1384
BstDEI CTNAG 6 cut(s) 29, 60, 840, 845, 1028, 1335
BstEII GGTNACC 1 cut(s) 889
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 5 cut(s) 178, 619, 983, 1141, 1358
BstKTI GATC 4 cut(s) 230, 554, 1034, 1323
BstMAI GTCTC 3 cut(s) 44, 470, 883
BstMBI GATC 4 cut(s) 227, 551, 1031, 1320
BstMWI GCNNNNNNNGC 5 cut(s) 64, 743, 764, 1237, 1362
BstNI CCWGG 3 cut(s) 187, 327, 611
BstNSI RCATGY 3 cut(s) 325, 1049, 1180
BstPI GGTNACC 1 cut(s) 889
BstSCI CCNGG 4 cut(s) 185, 325, 609, 881
BstSLI GKGCMC 2 cut(s) 517, 909
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 5 cut(s) 76, 229, 259, 262, 754
BstV2I GAAGAC 1 cut(s) 1419
BsuRI GGCC 4 cut(s) 58, 403, 438, 609
BtgZI GCGATG 1 cut(s) 673
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 5 cut(s) 178, 619, 983, 1141, 1358
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 4 cut(s) 56, 323, 826, 1384
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 8 cut(s) 222, 257, 302, 359, 401, 479, 494, 533
Csp6I GTAC 3 cut(s) 449, 970, 1255
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 6 cut(s) 103, 322, 374, 1020, 1046, 1177
CviQI GTAC 3 cut(s) 449, 970, 1255
DdeI CTNAG 6 cut(s) 29, 60, 840, 845, 1028, 1335
DpnI GATC 4 cut(s) 229, 553, 1033, 1322
DpnII GATC 4 cut(s) 227, 551, 1031, 1320
EaeI YGGCCR 1 cut(s) 607
Eco105I TACGTA 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 95, 1341
Eco31I GGTCTC 3 cut(s) 44, 470, 883
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 7 cut(s) 222, 257, 302, 359, 479, 494, 533
Eco57I CTGAAG 1 cut(s) 474
Eco91I GGTNACC 1 cut(s) 889
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 2 cut(s) 257, 302
EcoO65I GGTNACC 1 cut(s) 889
EcoRII CCWGG 3 cut(s) 185, 325, 609
EcoRV GATATC 2 cut(s) 124, 160
EcoT38I GRGCYC 2 cut(s) 95, 1341
FaeI CATG 6 cut(s) 106, 325, 377, 1023, 1049, 1180
FaqI GGGAC 2 cut(s) 1038, 1165
FatI CATG 6 cut(s) 102, 321, 373, 1019, 1045, 1176
Fnu4HI GCNGC 5 cut(s) 65, 243, 273, 276, 768
FokI GGATG 5 cut(s) 185, 626, 990, 1148, 1365
FriOI GRGCYC 2 cut(s) 95, 1341
Fsp4HI GCNGC 5 cut(s) 65, 243, 273, 276, 768
FspBI CTAG 4 cut(s) 519, 734, 936, 1383
GluI GCNGC 5 cut(s) 65, 243, 273, 276, 768
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 4 cut(s) 58, 403, 438, 609
HapII CCGG 3 cut(s) 207, 225, 882
Hin1II CATG 6 cut(s) 106, 325, 377, 1023, 1049, 1180
HinfI GANTC 9 cut(s) 12, 542, 684, 748, 914, 983, 1139, 1213, 1415
HpaII CCGG 3 cut(s) 207, 225, 882
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 4 cut(s) 583, 718, 1054, 1159
Hpy188I TCNGA 5 cut(s) 483, 629, 927, 1039, 1069
Hpy188III TCNNGA 9 cut(s) 9, 127, 135, 195, 357, 492, 531, 833, 1297
Hpy8I GTNNAC 4 cut(s) 583, 718, 1054, 1159
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 5 cut(s) 174, 225, 868, 1244, 1259
HpyCH4III ACNGT 1 cut(s) 1276
HpyCH4IV ACGT 3 cut(s) 45, 296, 1311
HpyCH4V TGCA 1 cut(s) 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 743, 764, 1237, 1362
HpyF3I CTNAG 6 cut(s) 29, 60, 840, 845, 1028, 1335
HpySE526I ACGT 3 cut(s) 45, 296, 1311
Hsp92II CATG 6 cut(s) 106, 325, 377, 1023, 1049, 1180
Kzo9I GATC 4 cut(s) 227, 551, 1031, 1320
LmnI GCTCC 4 cut(s) 182, 217, 710, 1362
Lsp1109I GCAGC 5 cut(s) 76, 229, 259, 262, 754
LweI GCATC 3 cut(s) 826, 1343, 1420
MaeI CTAG 4 cut(s) 519, 734, 936, 1383
MaeII ACGT 3 cut(s) 45, 296, 1311
MaeIII GTNAC 6 cut(s) 307, 418, 595, 889, 1120, 1223
MalI GATC 4 cut(s) 229, 553, 1033, 1322
MboI GATC 4 cut(s) 227, 551, 1031, 1320
MboII GAAGA 5 cut(s) 355, 400, 480, 810, 1424
MfeI CAATTG 2 cut(s) 200, 576
MhlI GDGCHC 5 cut(s) 95, 214, 517, 909, 1341
MlsI TGGCCA 1 cut(s) 609
MluCI AATT 8 cut(s) 200, 352, 430, 502, 560, 576, 814, 1146
MluNI TGGCCA 1 cut(s) 609
MlyI GAGTC 3 cut(s) 551, 1148, 1409
MnlI CCTC 9 cut(s) 230, 406, 414, 968, 1035, 1188, 1203, 1338, 1384
Mox20I TGGCCA 1 cut(s) 609
MroXI GAANNNNTTC 1 cut(s) 526
MscI TGGCCA 1 cut(s) 609
MseI TTAA 1 cut(s) 1098
Msp20I TGGCCA 1 cut(s) 609
MspI CCGG 3 cut(s) 207, 225, 882
MspR9I CCNGG 4 cut(s) 187, 327, 611, 883
MunI CAATTG 2 cut(s) 200, 576
MvaI CCWGG 3 cut(s) 187, 327, 611
MwoI GCNNNNNNNGC 5 cut(s) 64, 743, 764, 1237, 1362
NciI CCSGG 1 cut(s) 883
NdeII GATC 4 cut(s) 227, 551, 1031, 1320
NheI GCTAGC 1 cut(s) 1382
NlaIII CATG 6 cut(s) 106, 325, 377, 1023, 1049, 1180
NlaIV GGNNCC 5 cut(s) 7, 178, 303, 516, 1114
NmuCI GTSAC 3 cut(s) 418, 595, 1223
NspI RCATGY 3 cut(s) 325, 1049, 1180
PaeI GCATGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 58
PciI ACATGT 1 cut(s) 1176
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 6 cut(s) 12, 684, 748, 914, 983, 1213
PflMI CCANNNNNTGG 1 cut(s) 654
PfoI TCCNGGA 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 5 cut(s) 66, 244, 274, 277, 769
PleI GAGTC 3 cut(s) 550, 1147, 1409
PpsI GAGTC 3 cut(s) 550, 1147, 1409
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 2 cut(s) 257, 302
PscI ACATGT 1 cut(s) 1176
Psp5II RGGWCCY 2 cut(s) 257, 302
Psp6I CCWGG 3 cut(s) 185, 325, 609
PspEI GGTNACC 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 3 cut(s) 185, 325, 609
PspN4I GGNNCC 5 cut(s) 7, 178, 303, 516, 1114
PspPI GGNCC 8 cut(s) 222, 257, 302, 359, 401, 479, 494, 533
PspPPI RGGWCCY 2 cut(s) 257, 302
PsrI GAACNNNNNNTAC 2 cut(s) 1105, 1137
RsaI GTAC 3 cut(s) 450, 971, 1256
RsaNI GTAC 3 cut(s) 449, 970, 1255
SaqAI TTAA 1 cut(s) 1098
SatI GCNGC 5 cut(s) 65, 243, 273, 276, 768
Sau3AI GATC 4 cut(s) 227, 551, 1031, 1320
Sau96I GGNCC 8 cut(s) 222, 257, 302, 359, 401, 479, 494, 533
ScaI AGTACT 1 cut(s) 450
SchI GAGTC 3 cut(s) 551, 1148, 1409
ScrFI CCNGG 4 cut(s) 187, 327, 611, 883
SduI GDGCHC 5 cut(s) 95, 214, 517, 909, 1341
SfaNI GCATC 3 cut(s) 826, 1343, 1420
SinI GGWCC 7 cut(s) 222, 257, 302, 359, 479, 494, 533
SmlI CTYRAG 1 cut(s) 1388
SmoI CTYRAG 1 cut(s) 1388
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 325
Sse9I AATT 8 cut(s) 200, 352, 430, 502, 560, 576, 814, 1146
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 1 cut(s) 852
SspMI CTAG 4 cut(s) 519, 734, 936, 1383
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 4 cut(s) 185, 325, 609, 881
TaaI ACNGT 1 cut(s) 1276
TaiI ACGT 3 cut(s) 48, 299, 1314
TaqII GACCGA 1 cut(s) 270
TasI AATT 8 cut(s) 200, 352, 430, 502, 560, 576, 814, 1146
TatI WGTACW 1 cut(s) 448
TfiI GAWTC 6 cut(s) 12, 684, 748, 914, 983, 1213
Tru1I TTAA 1 cut(s) 1098
Tru9I TTAA 1 cut(s) 1098
TscAI CASTG 1 cut(s) 600
TseFI GTSAC 3 cut(s) 418, 595, 1223
TseI GCWGC 5 cut(s) 64, 242, 272, 275, 767
Tsp45I GTSAC 3 cut(s) 418, 595, 1223
TspDTI ATGAA 4 cut(s) 356, 572, 676, 808
TspRI CASTG 1 cut(s) 600
Van91I CCANNNNNTGG 1 cut(s) 654
VpaK11BI GGWCC 7 cut(s) 222, 257, 302, 359, 479, 494, 533
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 430, 502, 814
XceI RCATGY 3 cut(s) 325, 1049, 1180
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 4 cut(s) 519, 734, 936, 1383
ZrmI AGTACT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.