AT1G16860

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001653
Physical Location & Seq
Forward (+)
5767300 .. 5770602
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16860.1

Sequence Viewer

Length: 1425 bp
ATGGGTTCGAGATACCCATCTCATCAGCTCAGCAATGGGCTATTTGTGTCTGGTCGGCCTGAGCAACCCAAAGAAAGGGCTCCAACTATGAGTGCAGTAGCCATGCCTTACACTGGTGGTGATATCAAGAGATCAGGAGAATTGGGGAAGATGTTTGATATTCCAGCAGATGGGACTAAGTCCAGGAAGTCTGGTCCCATACCAGGTGCTCCTTCAAGGTCTGGCTCATTTGCAGGAACTGCACAATCAGGTCCAGGTGCTCCTATGGCCACTGGCCGTATGTCTGGCTCTTTAGCTTCTGCTGGCTCTGTTTCAATGAAGAAAACGAATTCCGGACCATTATCTAAGCATGGAGAGCCTTTGAAGAAATCATCTGGCCCTCAATCTGGTGGTGTAACTCGCCAAAATTCTGGTTCTATTCCTATTCTTCCAGCTACAGGTCTCATTACCTCTGGGCCTATTACATCAGGCCCTCTGAATTCATCTGGGGCTCCCAGGAAGGTTTCTGGTCCACTAGACTCTTCTGGTTTGATGAAGTCACATATGCCTACTGTTGTCCACAACCAGGCGGTAACTACCCTTGGTCCAGAAGATGACTTTTCCTGCCTGAAGAGCTTTCCAAAGCCTGTGCTGTGGCTGGTTGTTCTTATATTCATTATGGGGTTCCTTGCTGGAGGCTTCATTCTTGGTGCAGTTCACAACCCAATTCTCCTTGTTGTTGTCGCCATCTTGTTCACAGTTGTTGCTGCACTTTTCATTTGGAATATTTGTTGGGGAAGACGTGGTATCACAGATTTCATTGCTCGTTATCCTGATGCTGATCTTAGAACTGCCAAAAATGGTCAACACGTGAAGGTCACTGGGGTGGTAACTTGTGGTAATGTACCGCTTGAGTCATCCTTCCACAGAGTTCCCAGATGTGTGTACACATCGACATGTCTATATGAGTATCGTGGATGGGGTTCGAAACCAGCCAATTCTTCCCATCGTCACTTCACATGGGGACTGAGATCATCAGAGAGACATGTGGTGGACTTTTACATCTCTGATTTCCAGTCTGGGCTCAGAGCCTTAGTCAAAACCGGAAGCGGTGCAAAGGTAACACCTCTTGTAGATGATTCTGTTGTCATCGACTTCAAGCAAGGAAGCGAGCAAGTGTCTCCCGATTTTGTACGATGGTTAGGCAAGAAGAATCTAACAAGCGATGATCGAATAATGCGGCTCAAAGAAGGGTATATAAAAGAAGGAAGTACAGTGAGTGTGATTGGAGTGGTGCAGAGAAACGATAATGTGTTGATGATAGTTCCATCATCAGAGCCACTAGCAGCTGGTTGGCAATGGAGGAGATGCACATTCCCAACAAGTCTTGAAGGAATAGTATTGAGATGTGAAGACTCATCCAATGTTGATGCCATACCGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

474

Amino Acids

50.58

Weight (kDa)

9.67

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 170
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 4 cut(s) 569, 887, 1089, 1219
AcoI YGGCCR 2 cut(s) 267, 274
AcsI RAATTY 3 cut(s) 328, 406, 478
AcuI CTGAAG 1 cut(s) 629
AcvI CACGTG 1 cut(s) 850
AfaI GTAC 4 cut(s) 885, 926, 1173, 1252
AfiI CCNNNNNNNGG 7 cut(s) 75, 113, 170, 203, 386, 437, 565
AflIII ACRYGT 3 cut(s) 847, 935, 1024
AgeI ACCGGT 1 cut(s) 1417
AgsI TTSAA 5 cut(s) 216, 315, 364, 1138, 1370
AjiI CACGTC 1 cut(s) 782
AjnI CCWGG 5 cut(s) 182, 202, 253, 494, 564
AleI CACNNNNGTG 1 cut(s) 863
AluBI AGCT 5 cut(s) 28, 296, 434, 615, 1328
AluI AGCT 5 cut(s) 28, 296, 434, 615, 1328
Alw21I GWGCWC 2 cut(s) 211, 262
Alw26I GTCTC 3 cut(s) 446, 1015, 1164
AlwNI CAGNNNCTG 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 6 cut(s) 56, 267, 274, 376, 455, 469
ApeKI GCWGC 2 cut(s) 746, 1325
ApoI RAATTY 3 cut(s) 328, 406, 478
AsiGI ACCGGT 1 cut(s) 1417
AspS9I GGNCC 8 cut(s) 194, 251, 335, 377, 455, 470, 509, 584
AsuHPI GGTGA 1 cut(s) 131
AsuII TTCGAA 1 cut(s) 965
AvaII GGWCC 5 cut(s) 194, 251, 335, 509, 584
BalI TGGCCA 1 cut(s) 269
BanII GRGCYC 3 cut(s) 82, 493, 1065
BbrPI CACGTG 1 cut(s) 850
BbsI GAAGAC 2 cut(s) 784, 1398
Bbv12I GWGCWC 2 cut(s) 211, 262
BbvI GCAGC 2 cut(s) 733, 1337
BccI CCATC 7 cut(s) 25, 164, 734, 951, 993, 1170, 1315
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 5 cut(s) 184, 204, 255, 496, 566
BcoDI GTCTC 3 cut(s) 446, 1015, 1164
BfaI CTAG 2 cut(s) 515, 1322
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 3 cut(s) 747, 1220, 1326
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 3 cut(s) 748, 1221, 1327
Bme1390I CCNGG 5 cut(s) 184, 204, 255, 496, 566
Bme18I GGWCC 5 cut(s) 194, 251, 335, 509, 584
BmgBI CACGTC 1 cut(s) 782
BmgT120I GGNCC 8 cut(s) 194, 251, 335, 377, 455, 470, 509, 584
BmiI GGNNCC 4 cut(s) 81, 196, 492, 665
BmrFI CCNGG 5 cut(s) 184, 204, 255, 496, 566
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 3 cut(s) 805, 1337, 1399
BmuI ACTGGG 1 cut(s) 870
BpiI GAAGAC 2 cut(s) 784, 1398
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 965
BpuEI CTTGAG 1 cut(s) 911
BsaAI YACGTR 1 cut(s) 850
BsaBI GATNNNNATC 2 cut(s) 16, 819
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 494, 580
BsaWI WCCGGW 3 cut(s) 332, 1082, 1417
Bsc4I CCNNNNNNNGG 7 cut(s) 75, 113, 170, 203, 386, 437, 565
Bse118I RCCGGY 1 cut(s) 1417
Bse1I ACTGG 4 cut(s) 118, 277, 865, 1054
Bse3DI GCAATG 3 cut(s) 40, 798, 1343
Bse8I GATNNNNATC 2 cut(s) 16, 819
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 5 cut(s) 184, 204, 255, 496, 566
BseDI CCNNGG 2 cut(s) 494, 580
BseGI GGATG 3 cut(s) 896, 962, 1397
BseJI GATNNNNATC 2 cut(s) 16, 819
BseLI CCNNNNNNNGG 7 cut(s) 75, 113, 170, 203, 386, 437, 565
BseMI GCAATG 3 cut(s) 40, 798, 1343
BseMII CTCAG 4 cut(s) 43, 51, 998, 1078
BseNI ACTGG 4 cut(s) 118, 277, 865, 1054
BseRI GAGGAG 1 cut(s) 1357
BseXI GCAGC 2 cut(s) 733, 1337
BsgI GTGCAG 5 cut(s) 114, 225, 711, 732, 1295
BshFI GGCC 6 cut(s) 58, 269, 276, 378, 457, 471
BshTI ACCGGT 1 cut(s) 1417
BsiHKAI GWGCWC 2 cut(s) 211, 262
BsiSI CCGG 3 cut(s) 333, 1083, 1418
BslFI GGGAC 3 cut(s) 180, 187, 1017
BslI CCNNNNNNNGG 7 cut(s) 75, 113, 170, 203, 386, 437, 565
BsmAI GTCTC 3 cut(s) 446, 1015, 1164
BsmFI GGGAC 3 cut(s) 180, 187, 1017
BsnI GGCC 6 cut(s) 58, 269, 276, 378, 457, 471
Bso31I GGTCTC 1 cut(s) 446
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 5 cut(s) 82, 211, 262, 493, 1065
Bsp13I TCCGGA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 4 cut(s) 131, 820, 1010, 1207
Bsp1720I GCTNAGC 1 cut(s) 29
BspACI CCGC 4 cut(s) 569, 887, 1089, 1219
BspANI GGCC 6 cut(s) 58, 269, 276, 378, 457, 471
BspCNI CTCAG 4 cut(s) 42, 52, 999, 1077
BspEI TCCGGA 1 cut(s) 332
BspLI GGNNCC 4 cut(s) 81, 196, 492, 665
BspQI GCTCTTC 1 cut(s) 605
BspT104I TTCGAA 1 cut(s) 965
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 3 cut(s) 40, 798, 1343
BsrFI RCCGGY 1 cut(s) 1417
BsrGI TGTACA 1 cut(s) 924
BsrI ACTGG 4 cut(s) 118, 277, 865, 1054
BssAI RCCGGY 1 cut(s) 1417
BssECI CCNNGG 2 cut(s) 494, 580
BssMI GATC 4 cut(s) 131, 820, 1010, 1207
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 5 cut(s) 184, 204, 255, 496, 566
Bst4CI ACNGT 3 cut(s) 553, 739, 1255
Bst6I CTCTTC 2 cut(s) 526, 605
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 924
BstBAI YACGTR 1 cut(s) 850
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 2 cut(s) 304, 1151
BstDEI CTNAG 8 cut(s) 29, 60, 177, 345, 824, 1007, 1064, 1072
BstF5I GGATG 3 cut(s) 896, 962, 1397
BstKTI GATC 4 cut(s) 134, 823, 1013, 1210
BstMAI GTCTC 3 cut(s) 446, 1015, 1164
BstMBI GATC 4 cut(s) 131, 820, 1010, 1207
BstMWI GCNNNNNNNGC 4 cut(s) 239, 266, 355, 612
BstNI CCWGG 5 cut(s) 184, 204, 255, 496, 566
BstNSI RCATGY 2 cut(s) 939, 1028
BstSCI CCNGG 5 cut(s) 182, 202, 253, 494, 564
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 733, 1337
BstV2I GAAGAC 2 cut(s) 784, 1398
BstXI CCANNNNNNTGG 1 cut(s) 410
BsuRI GGCC 6 cut(s) 58, 269, 276, 378, 457, 471
BtgZI GCGATG 1 cut(s) 1218
BtrI CACGTC 1 cut(s) 782
BtsCI GGATG 3 cut(s) 896, 962, 1397
BtsIMutI CAGTG 4 cut(s) 111, 270, 858, 1260
Cac8I GCNNGC 2 cut(s) 304, 1151
CaiI CAGNNNCTG 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 1417
Cfr13I GGNCC 8 cut(s) 194, 251, 335, 377, 455, 470, 509, 584
CsiI ACCWGGT 1 cut(s) 202
Csp6I GTAC 4 cut(s) 884, 925, 1172, 1251
CspAI ACCGGT 1 cut(s) 1417
CviAII CATG 5 cut(s) 103, 350, 936, 999, 1025
CviQI GTAC 4 cut(s) 884, 925, 1172, 1251
DdeI CTNAG 8 cut(s) 29, 60, 177, 345, 824, 1007, 1064, 1072
DpnI GATC 4 cut(s) 133, 822, 1012, 1209
DpnII GATC 4 cut(s) 131, 820, 1010, 1207
EaeI YGGCCR 2 cut(s) 267, 274
Eam1104I CTCTTC 2 cut(s) 526, 605
EarI CTCTTC 2 cut(s) 526, 605
Eco130I CCWWGG 1 cut(s) 580
Eco24I GRGCYC 3 cut(s) 82, 493, 1065
Eco31I GGTCTC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 5 cut(s) 194, 251, 335, 509, 584
Eco57I CTGAAG 1 cut(s) 629
Eco72I CACGTG 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 328, 478
EcoRII CCWGG 5 cut(s) 182, 202, 253, 494, 564
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 580
EcoT38I GRGCYC 3 cut(s) 82, 493, 1065
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 5 cut(s) 106, 353, 939, 1002, 1028
FaqI GGGAC 3 cut(s) 180, 187, 1017
FatI CATG 5 cut(s) 102, 349, 935, 998, 1024
FauNDI CATATG 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 747, 1220, 1326
FokI GGATG 3 cut(s) 883, 969, 1384
FriOI GRGCYC 3 cut(s) 82, 493, 1065
Fsp4HI GCNGC 3 cut(s) 747, 1220, 1326
FspBI CTAG 2 cut(s) 515, 1322
GluI GCNGC 3 cut(s) 747, 1220, 1326
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 6 cut(s) 58, 269, 276, 378, 457, 471
HapII CCGG 3 cut(s) 333, 1083, 1418
Hin1II CATG 5 cut(s) 106, 353, 939, 1002, 1028
HincII GTYRAC 1 cut(s) 845
HindII GTYRAC 1 cut(s) 845
HinfI GANTC 5 cut(s) 518, 893, 1118, 1192, 1394
HpaII CCGG 3 cut(s) 333, 1083, 1418
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 8 cut(s) 512, 559, 697, 735, 845, 925, 927, 1033
Hpy188I TCNGA 5 cut(s) 477, 1018, 1048, 1067, 1315
Hpy188III TCNNGA 8 cut(s) 9, 127, 135, 333, 587, 812, 1163, 1367
Hpy8I GTNNAC 8 cut(s) 512, 559, 697, 735, 845, 925, 927, 1033
HpyAV CCTTC 7 cut(s) 222, 493, 847, 910, 1223, 1238, 1364
HpyCH4III ACNGT 3 cut(s) 553, 739, 1255
HpyCH4IV ACGT 2 cut(s) 781, 849
HpyCH4V TGCA 8 cut(s) 95, 233, 242, 692, 749, 1094, 1276, 1350
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 266, 355, 612
HpyF3I CTNAG 8 cut(s) 29, 60, 177, 345, 824, 1007, 1064, 1072
HpySE526I ACGT 2 cut(s) 781, 849
Hsp92II CATG 5 cut(s) 106, 353, 939, 1002, 1028
Kpn2I TCCGGA 1 cut(s) 332
Kzo9I GATC 4 cut(s) 131, 820, 1010, 1207
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 4 cut(s) 85, 214, 265, 496
Lsp1109I GCAGC 2 cut(s) 733, 1337
LweI GCATC 3 cut(s) 805, 1337, 1399
MabI ACCWGGT 1 cut(s) 202
MaeI CTAG 2 cut(s) 515, 1322
MaeII ACGT 2 cut(s) 781, 849
MaeIII GTNAC 7 cut(s) 394, 537, 571, 856, 868, 989, 1099
MalI GATC 4 cut(s) 133, 822, 1012, 1209
MboI GATC 4 cut(s) 131, 820, 1010, 1207
MhlI GDGCHC 5 cut(s) 82, 211, 262, 493, 1065
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 6 cut(s) 140, 328, 406, 478, 705, 976
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 3 cut(s) 512, 902, 1388
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 6 cut(s) 390, 460, 483, 668, 1116, 1335
Mox20I TGGCCA 1 cut(s) 269
MroI TCCGGA 1 cut(s) 332
MscI TGGCCA 1 cut(s) 269
MslI CAYNNNNRTG 2 cut(s) 863, 934
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 1328
MspI CCGG 3 cut(s) 333, 1083, 1418
MspR9I CCNGG 5 cut(s) 184, 204, 255, 496, 566
MvaI CCWGG 5 cut(s) 184, 204, 255, 496, 566
MwoI GCNNNNNNNGC 4 cut(s) 239, 266, 355, 612
NdeI CATATG 1 cut(s) 543
NdeII GATC 4 cut(s) 131, 820, 1010, 1207
NlaIII CATG 5 cut(s) 106, 353, 939, 1002, 1028
NlaIV GGNNCC 4 cut(s) 81, 196, 492, 665
NmuCI GTSAC 3 cut(s) 537, 856, 989
NspI RCATGY 2 cut(s) 939, 1028
NspV TTCGAA 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 863
PciI ACATGT 2 cut(s) 935, 1024
PciSI GCTCTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 1118, 1192
PflFI GACNNNGTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 182
PinAI ACCGGT 1 cut(s) 1417
PkrI GCNGC 3 cut(s) 748, 1221, 1327
PleI GAGTC 3 cut(s) 512, 901, 1388
PmaCI CACGTG 1 cut(s) 850
PmlI CACGTG 1 cut(s) 850
PpsI GAGTC 3 cut(s) 512, 901, 1388
Ppu21I YACGTR 1 cut(s) 850
PscI ACATGT 2 cut(s) 935, 1024
Psp6I CCWGG 5 cut(s) 182, 202, 253, 494, 564
PspCI CACGTG 1 cut(s) 850
PspGI CCWGG 5 cut(s) 182, 202, 253, 494, 564
PspN4I GGNNCC 4 cut(s) 81, 196, 492, 665
PspPI GGNCC 8 cut(s) 194, 251, 335, 377, 455, 470, 509, 584
PstNI CAGNNNCTG 1 cut(s) 239
PsyI GACNNNGTC 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 1328
RsaI GTAC 4 cut(s) 885, 926, 1173, 1252
RsaNI GTAC 4 cut(s) 884, 925, 1172, 1251
RseI CAYNNNNRTG 2 cut(s) 863, 934
SapI GCTCTTC 1 cut(s) 605
SatI GCNGC 3 cut(s) 747, 1220, 1326
Sau3AI GATC 4 cut(s) 131, 820, 1010, 1207
Sau96I GGNCC 8 cut(s) 194, 251, 335, 377, 455, 470, 509, 584
SchI GAGTC 3 cut(s) 512, 902, 1388
ScrFI CCNGG 5 cut(s) 184, 204, 255, 496, 566
SduI GDGCHC 5 cut(s) 82, 211, 262, 493, 1065
SexAI ACCWGGT 1 cut(s) 202
SfaNI GCATC 3 cut(s) 805, 1337, 1399
SfcI CTRYAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 965
SinI GGWCC 5 cut(s) 194, 251, 335, 509, 584
SmiMI CAYNNNNRTG 2 cut(s) 863, 934
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
Sse9I AATT 6 cut(s) 140, 328, 406, 478, 705, 976
SsiI CCGC 4 cut(s) 569, 887, 1089, 1219
SspI AATATT 1 cut(s) 766
SspMI CTAG 2 cut(s) 515, 1322
StyD4I CCNGG 5 cut(s) 182, 202, 253, 494, 564
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 3 cut(s) 553, 739, 1255
TaiI ACGT 2 cut(s) 784, 852
TaqI TCGA 5 cut(s) 8, 932, 965, 1131, 1210
TasI AATT 6 cut(s) 140, 328, 406, 478, 705, 976
TatI WGTACW 2 cut(s) 924, 1250
TauI GCSGC 1 cut(s) 1222
TfiI GAWTC 2 cut(s) 1118, 1192
TscAI CASTG 4 cut(s) 118, 277, 865, 1260
TseFI GTSAC 3 cut(s) 537, 856, 989
TseI GCWGC 2 cut(s) 746, 1325
Tsp45I GTSAC 3 cut(s) 537, 856, 989
TspDTI ATGAA 7 cut(s) 332, 471, 548, 643, 670, 745, 787
TspRI CASTG 4 cut(s) 118, 277, 865, 1260
Tth111I GACNNNGTC 1 cut(s) 178
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 5 cut(s) 194, 251, 335, 509, 584
XapI RAATTY 3 cut(s) 328, 406, 478
XceI RCATGY 2 cut(s) 939, 1028
XspI CTAG 2 cut(s) 515, 1322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.